Les Ranunculales sont un ordre d’angiospermes d’environ 4500 espèces, incluant des plantes communes comme les boutons d’or et les coquelicots. Leurs fleurs sont très diversifiées et ont piqué l’intérêt des botanistes depuis des décennies. Les sept familles de l’ordre sont faciles à reconnaître sur le terrain, mais paradoxalement, certaines familles n’ont pas de synapomorphies et ont des états ancestraux peu clairs, comme les Ranunculaceae (~ 2500 espèces). Cette diversité florale peut être liée à la pollinisation et des innovations ont pu être guidées par cette interaction. De plus, les plantes de ce groupe produisent une grande variété de composés secondaires, certains dont les propriétés sont connues depuis fort longtemps (comme la morphine et le curare). Ces substances pourraient jouer un rôle dans la défense contre les herbivores. Les caractères floraux et chimiques sont donc de bons candidats pour produire des hypothèses adaptatives. Ici nous utilisons des méthodes analytiques modernes pour comprendre les patrons d’évolution expliquant la distribution actuelle de la diversité et l’évolution des fleurs et des toxines de Ranunculales. Un nouveau cadre phylogénétique synthétique a été produit avec des séquences publiées de 144 espèces. Seize caractères floraux et la présence de certains composés métaboliques ont été reconstruits sur cet arbre avec la méthode de parcimonie et la méthode de « Reversible Jump Monte Carlo Markov Chains ». Un nouveau scénario d’évolution florale a été produit, où les Ranunculales avaient une fleur ancestrale trimère avec trois cycles de tépales et où les ancêtres des familles ont évolué en perdant ou en différenciant des cycles du périanthe. Les patrons d’évolution des toxines montrent de la variabilité, certaines apparaissant et disparaissant aléatoirement (comme les saponines), d’autres étant des synapomorphies bien soutenues de certains clades (comme les diterpènes de type aconitine pour la tribu des Delphinieae). Ces résultats offrent une meilleure compréhension de l’histoire naturelle des Ranunculales. En outre, cet ordre est le groupe-frère de toutes les autres eudicotylédones, et a donc une position clef pour comprendre l’évolution précoce de ce clade. Ces résultats vont fournir une compréhension plus profonde des changements floraux et phytochimiques qui ont eu lieu à la base des eudicotylédones, qui contiennent 70% des angiospermes actuelles. / Ranunculales are an order of angiosperms comprising ca. 4,500 species including common plants like buttercups and poppies. Their flowers are highly diversified and have raised the interest of botanists for decades. Each of the seven families of the order is easily recognizable in the field, but paradoxically some families lack floral synapomorphies and have unclear ancestral states, like Ranunculaceae (ca. 2,500 species). This floral diversity may be linked to pollination and innovations may have been driven by this interaction. In addition, the plants of this group produce a variety of secondary compounds, some of which having been known for ages for their properties, like morphine or curare. Those substances could play a role in defense against herbivores. Both floral and chemical characters are thus good candidates to produce adaptive hypotheses. Here we used modern analytical methods to understand the the evolutionary patterns accounting for the extant distribution of diversity and evolution of Ranunculales flowers and toxins. A new synthetic phylogenetic framework of the Ranunculales based on already available sequences of 144 species was produced. Sixteen characters of the flower, and the presence of selected secondary metabolites were reconstructed on this tree using parsimony, and Reversible Jump Monte Carlo Markov Chains. A new scenario for floral evolution was produced, where Ranunculales had a trimerous perianth with three whorls of tepals ancestor, and the ancestors of the families evolved by losing and/or differentiating perianth whorls. The evolutionary patterns of toxins showed variability, some appearing and disappearing randomly (such as saponins), others being well-supported synapomorphies (such as aconitine-like diterpens for the tribe Delphinieae). These results offer a better understanding of the natural history of Ranunculales. Furthermore, this order is the sister-group of all other eudicots, and thus has a key position to understand the early evolution of this clade. These results will provide a deeper understanding of the floral and chemical changes that took place at the base of eudicots, which contains 70% of living angiosperms.

The purpose of this thesis was to gain an insight into the macrobotanical subsistence practices of Nancy Patterson Village and see how those practices fit in with the practices of the general Mesa Verde region by analyzing the burnt macrobotanical remains found in processed flotation samples. Previous work done at Nancy Patterson Village showed a shift in the faunal subsistence practices to a greater reliance on domesticated turkey during the Pueblo III period. However, the macro botanical analysis showed a higher richness of wild plant taxa in the Pueblo III period when compared to Pueblo II. The change to a higher richness of plant taxa in the later period is attributed to the changes in social and environmental climates causing difficulties in sustaining the population. These difficulties pushed the inhabitants to expand their selection of plant types used for food. Despite the higher richness of plant taxa in Pueblo III, other sites from the Central Mesa Verde region had higher richness. However, Nancy Patterson Village used the smaller number of wild plants types more intensely than the other sites from the region. No explanation was found to explain this difference.

Nickel enzymes have been suggested, through numerous phylogenetic studies, to have been among the very first catalytic compounds on the early Earth, possibly present in the last universal common ancestor (LUCA) or prior to the onset of life. This is because of the type of reactions catalyzed by some of these enzymes, the nature of organisms that utilize them, their distribution in the tree of life, and their key roles in what is now thought of as possibly one of the oldest carbon fixation pathways, the Wood-Ljungdahl (WL) pathway. Additionally, nickel is generally thought to have been an abundant element on the early Earth, highly soluble in what were, theoretically, euxinic (anoxic and sulfidic) ocean waters. This combined with the fact that the enzymes involved in the WL pathway have an active center configuration that resembles that of minerals found in hydrothermal vent walls, makes nickel enzymes a likely candidate to have evolved from what were proto-enzymes, responsible for the prebiotic catalysis of the first simple organic molecules prior to the origins of life, according to the so-called submarine alkaline hydrothermal vent theory, first presented by Michael J. Russell in 1993 (Russell et al. 1994). In this study, I expand the known coverage on the distribution of these enzymes by mapping them in 10,575 OTUs of microbial taxa. Using their pattern of distribution, I reconstruct their histories along the branches of a reference phylogenetic tree of the same taxa through methods of ancestral reconstruction of discrete traits. Additionally, I construct an individual gene tree for each of the enzymes in order to consolidate gene history with species history. My results showed that the redox nickel enzymes (except methyl-coenzyme M reductase) are ancestral to all prokaryotes, while non-redox enzymes are derived and with multiple origins, possibly due to lateral gene transfer events or convergent evolution. I propose that the patterns observed are a product of the drastic changes during early Earth history, namely a hypothesized “nickel famine” or the Great Oxidation Event, which acted as selective pressures.

Amongst the Iron Age Scandinavian elite, there are several supra-regional and multifaceted tripartite (or threefold) symbolic expressions. These include expressions found in art, artefacts and monuments, such as the triangular stone-settings, or Sw. treuddar, which may be the strongest manifestation in the landscape. In addition, tripartite symbolism is found in the elite’s óðal-claims and also Norse mythological structures. Due to the widespread pervasiveness of tripartite symbology within the culture of the Iron Age elite, these phenomena are conceptualised in the theoretical framework of a ‘tripartite ideology’. This study addresses the questions of why was the tripartite ideology so enduring within the Nordic Iron Age, in what ways did it manifest and what positions did it hold in the Iron Age elite’s socio-cultural and religious thought-world? This research examines the monumental, artefactual, social and mythological manifestations of the tripartite ideology in Iron Age Scandinavia. The objective is to formulate a theory which synthesises the various expressions of tripartite symbology using a source-pluralistic methodology, which combines archaeological evidence with both emic (insider) and etic (outsider) historical sources, alongside religious studies and semiotics in order to provide a more representative picture of the function of treuddar and tripartite symbolism in the Iron Age elite milieu. The result of this methodology is that the tripartite ideology is connected with the Iron Age elite’s ancestral óðal-claims based on a legendary or divine descent, along with acting as a‘liminal locus’ whereby the Other World could be accessed.

This study set out to express, purify and characterize twelve ammonia lyase enzymes for potential application as a supplement to a treatment of an inborn error of metabolism disease. The DNA sequence for two wild-type ammonia lyases, three modified ammonia lyases and seven resurrected ancestral ammonia lyases had been synthesized and cloned in vectors. These were transformed into Escherichia coli, expressed, purified using immobilized metal affinity chromatography and size exclusion chromatography and characterized. Ten of the enzymes were successfully expressed and purified. All enzymes had a higher turnover number with substrate 1 than with substrate 2. The wild-types showed the highest catalytic turnover and one of them displayed substrate cooperativity. The modified enzymes were inactive. Some ancestral enzymes were active and had decreasing kcat with age. A promising ancestral enzymes was found that showed a kcat of 2,85 s-1 with substrate 1 and 1,82 s-1 with substrate 2. The ancestral enzymes had a lower Km with substrate 2 compared to substrate 1, while one of the wild-types had a higher Km with substrate 2 than with substrate 1, indicating that the substrate affinity has switched. The ancestral enzymes had increased thermostability compared to the wild-types which increased with age. Ranging from a +7C increase in melting temperature with the youngest ancestral enzyme to +10,7C with the oldest tested enzyme, comparing with one of the wild-types. The promising ancestral enzyme displayed a higher stability than the wild-types during long term incubation in 37_C and 25_C, since it did not become prone to aggregation,it did not show visible degradation on SDS-PAGE and it retained the highest activity following incubation. It was also demonstrated that neither wild-types nor the promising ancestral enzyme were stable in a simulated gut environment. The promising ancestral enzyme and one of the wild-types degraded substrate 1 and 2 in serum. Using the resurrection of ancestral sequences a promising enzyme has been produced and characterized, displaying properties that are desired in therapeutic enzymes. The enzyme did not aggregate or become prone to aggregation over time, it was thermostable, it was active in serum and had acceptable catalytic properties. For therapeutic application of the ancestral enzyme, immunogenicty should be analyzed in silico and in vitro followed by further investigation in vivo. / Målet med denna studie var att uttrycka, rena och karaktärisera tolv ammonia lyase enzymer, för potentiell användning som komplement till en behandling utav en sjukdom, som tillhör sjukdomsgruppen medfödda ämnesomsättningsrubbningar. DNA sekvensen för två vild-typammonia lyaser, tre modifierade ammonia lyaser och sju återuppväckta ammonia lyaser hade blivit syntetiserade och klonade i vektorer. E.coli celler blev transformerade med vektorerna, vilka uttryckte enzymerna, som renades med hjälp av immobilized metal affinity chromatography och gelfiltrering och karaktäriserades. Tio utav enzymerna kunde uttryckas och renas. Alla enzymer hade högre katalytisk omsättning av substrat 1 än substrat 2. Vildtyperna hade högst kcat med båda substrat och en utav dem uppvisade substratsammarbete. De modifierade enzymerna var inaktiva. Några av de återuppväckta ammonia lyaserna var aktiva och kcat minskade med ålder. Ett av de återuppväckta enzymerna var lovande och hade ett kcat värde av 2,85 s-1 med substrat 1 och 1,82 s-1 med substrat 2. De återuppväckta enzymerna hade ett lägre Km värde för substrat 2 än substrat 1, jämfört med en utav vildtyperna som hade ett högre Km värde för substrat 2 än substrat 1, vilket indikerar ett skifte i substrataffinitet. De återuppväckta enzymerna var mer termostabilia än vild-typerna och termostabiliteten ökar med ålder. Ökningen i smälttemperatur låg i spannet av +7C för de yngsta återuppväckta enzymerna till + 10,7C för det äldsta testade återuppväckta enzymet, vid jämförelse med en utav vild-typerna. Det lovande återuppväckta enzymet demonstrerade även en högre stabilitet än vild-typerna under långtidsinkubering, eftersom den inte blev benägen att aggregera, den uppvisade ingen nedbrytning på SDS-PAGE och den behöll högst aktivitet efter inkubering. Det bevisades även att varken vild-typerna eller det lovande återuppväckta enzymet var stabila i en simulerad magsäcksmiljö. Både det lovande återuppväckta enzymet och en av vild-typerna bröt ner substrat 1 och 2 i serum. Genom att återuppväcka sekvenser kunde ett lovande enzym produceras och karaktäriseras, vilket uppvisade egenskaper som är eftertraktade i terapeutiska enzymer. Enzymet aggregerade ej, det blev inte benäget att aggregera över tid, det var termostabilt, det var aktivt i serum och hade acceptabla katalytiska egenskaper. För terapeutisk applikation av det återuppväckta enzymet, borde analys av dess immunogenicitet utföras in silico och in vitro följt av vidare undersökning in vivo.

This study assessed ways compositional analysis, facilitated by portable X-ray fluorescence technology (PXRF), can be applied in the museum setting to resolve provenance issues and other collections management questions. A major segment of the study evaluated PXRF as a non-invasive geochemical analysis technique to address concerns about whether the resolution of results is sufficient to draw meaningful conclusions. Compositional analysis, mainly facilitated by PXRF, was successfully applied to three clay artifact collections from the southwestern United States. Pottery sherds from Fourmile Ruin were analyzed using PXRF and compared to analyses from invasive wavelength-dispersive XRF and X-ray diffraction techniques. Expanding the data associated with the artifacts increases the collection's research value. The results of hierarchical clustering suggest further compositional analysis of Fourmile Ruin ceramics to verify this study's conclusions and evaluate current assumptions regarding where certain wares are produced. The iconic Pilling figurine collection was analyzed using scanning electron microscopy and PXRF to evaluate the authenticity of a figurine that had been returned after being lost for forty years. The geochemical tests confirmed the results from the basketry-imprint analysis that the returned figurine was the missing figurine, allowing the museum to restore the artifact's provenance. A pot being offered as a potential donation was analyzed using PXRF to evaluate if there was evidence of forgery or previous repair and help the museum decide whether or not to accept the donation. The study concluded the vessel has not been constructed or partially reconstructed using plaster of Paris. If the vessel has been constructed or partially reconstructed using pieced-together pottery sherds, they all came from a similar clay source. Based on these results, the museum decided to accept the pot as a donation. This study demonstrates the viability of PXRF as a useful geochemical research technique, particularly in cases where higher resolution invasive and destructive analysis techniques are not permitted. It establishes that PXRF can be used to authenticate and restore provenance both within a collection of objects and within a single object. Compositional analysis facilitated by PXRF can be a valuable tool in museum collections management and research.

Capoeira is an African diasporic art form that developed in Brazil during the transatlantic slave trade. This research explores the history of Capoeira and its contemporary engagement through an autoethnographic method. It follows the first-hand experience of being a black female researcher and a Capoeirista both in Europe and Brazil. The purpose of engaging with this particular perspective was to recentre the ways in which we view Capoeira and its history, understanding that it has many embodiments of resistance that also include Black women. Embodiments here are framed as the embedded identities and corporeality affected by culture and society over several generations. Through this process, we open our awareness of further embodiments of the individual, together with that of the practice. Inspired by bell hooks' essay ‘Choosing the Margin as a Space of Radical Openness’, this thesis is a conversation between the past, the present and the future, it interrogates the popular structures in Capoeira that have been upheld or embodied and as a result has separated us from ancestral knowledge and knowledge of self. The ritual of Capoeira is mirrored throughout this thesis in order to engage with the intrinsic values of the practice and bring about transformative ways of seeing and moving in the world around us.

No description available.

Ancestral Sequence Reconstruction is a technique used to statistically infer the most likely ancestor of a set of evolutionarily related sequences, but research which relies solely on protein data has the disadvantage of sequence information being lost upon translation of a protein from its gene transcript, due to the redundancy inherent in the genetic code. In this project, the amino acid sequences, and separately the corresponding codon sequences, of 184 homologous Acetylcholine receptor protein sequences were aligned, and phylogenetic analysis and ancestral sequence reconstruction was performed based on both alignments to infer several ancestral sequences representing important milestones in the evolutionary history of the homologous protein family. To further extract meaningful information from the nucleotide sequences, positive selection analysis was performed on the codon alignment using the Mixed Effects Model of Evolution method, which estimates and compares between the rates of synonymous and non- synonymous mutations across the alignment to detect the occurrence of positive selection events throughout their evolution. The Mixed Effects Model of Evolution can infer positive selection across both sites and evolutionary branches in a sequence alignment, thus highlighting residues along the evolutionary trajectory of the proteins which may have been functionally important in their evolution. Positive selection analysis detected positive selection at a multitude of sites and branches, and by mapping signatures at which selection is strongest with changes in the trajectory of ancestral states, several important sites were chosen as likely to be most valuable for future experimental testing. The implications of this study on the benefits of conducting ancestral sequence reconstruction with protein and codon sequences are discussed.

In K-12 education, student voice is not often considered when establishing curriculum, addressing school policy, or creating a climate that reflects culturally inclusive teaching practices. Across education systems serving Indigenous communities within the United States, the voices of youth as a form of contribution are silenced, which echoes further attempts towards the erasure of the existence of these communities. The context of the study highlighted various strengths through their traditions, languages, and cultural teachings. While Indigenous communities exist globally, this study centered on Indigenous communities from the North American continent, specifically those Native to the Hawaiian Islands. To learn about the lived experiences of Indigenous youth’s strengths embedded in cultural assets, the following research questions guided this qualitative study: 1) What wisdom and cultural practices do Indigenous youth carry with them into schools? and 2) In what ways can educators support youth voice through culturally sustaining and revitalizing pedagogical practices? This research leveraged Yosso’s (2005) Community Cultural Wealth framework and McCarty and Leeʻs (2014) Culturally Sustaining and Revitalizing Pedagogy to highlight diverse forms of capital embedded within Indigenous youth. Analysis revealed significant themes related to various forms of cultural wisdom and knowledge in that embody the circle of reciprocity, community, Indigenous forms of artistry, and advocacy. Recommendations for integrating culturally sustainable and revitalizing pedagogical practices are provided for school administrators and educators.