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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
91

Mending

Jones, Tacie 03 December 2019 (has links)
Mending is a body of artwork created in response to ancestral trauma inherited between women. This paper discusses the exhibition of work, which consists of media installation, sculpture, and photography. Mending confronts Walter Benjamin’s patriarchal argument that one must intellectually excavate deep memory. Rather, the processes used to create the body of work engage a sensorial approach, and attempt to both reconstruct embodied memory and reconcile trauma. The act of mending is an historically feminine gesture appropriate for resolving the transgenerational trauma of the female body’s experience. Additionally, the media serves as witness, and has the potential to act as an impartial observer in the process of unraveling embodied trauma, allowing for reflexive self-witness. Overall, Mending rejects the thought-centric process of excavation, instead centering sensory-based spiritual practices in contemporary art related to nature immersion, meditative ritual, and collaboration between women working to heal handed-down victimization. / Mending is a body of artwork created in response to ancestral trauma inherited between women. This paper discusses the exhibition of work, which consists of media installation, sculpture, and photography. Mending confronts Walter Benjamin’s patriarchal argument that one must intellectually excavate deep memory. Rather, the processes used to create the body of work engage a sensorial approach, and attempt to both reconstruct embodied memory and reconcile trauma. The act of mending is an historically feminine gesture appropriate for resolving the transgenerational trauma of the female body’s experience. Additionally, the media serves as witness, and has the potential to act as an impartial observer in the process of unraveling embodied trauma, allowing for reflexive self-witness. Overall, Mending rejects the thought-centric process of excavation, instead centering sensory-based spiritual practices in contemporary art related to nature immersion, meditative ritual, and collaboration between women working to heal handed-down victimization.
92

台灣祭祀公業權屬爭議問題之研究

翁崇岳, Weng, Chung Yueh Unknown Date (has links)
摘要 「祭祀公業」乃基於傳統習慣所形成擁有特殊性之「共有」,係以祭祀祖先為目的所設立之獨立財產。本文研究乃以祭祀公業條例第五十條規範過去所設立祭祀公業法人與現行民法成立之財團法人(宗祠財團法人)其設立目的及宗旨是否相同,又本條例針對過去設立之祭祀公業法人格之取得及未取得法人格時土地權屬應如何處理,及其條例規定衍生派下現員權屬爭議、及男女平權繼承是否妥當,予以分析祭祀公業權屬爭議問題。試圖從慎終追遠及延續傳統宗族舊慣的角度,以分析祭祀公業之權屬結構,盼能指出台灣從過去到現在實施清理祭祀公業政策之盲點,俾利政府實施祭祀公業清理制度的處理原則及法令修訂時的參考。 本文第二章有關祭祀公業及其相關規定之探討:就祭祀公業緣起、意義、制度沿革、派下的權利與義務及權屬爭議之解釋、判決、判例、規定加以彙整,並以祭祀公業之法律性質、派下員申報及登記、廢止解散等權屬問題之予以論述;第三章祭祀公業主體性問題之探討,首先是應探究祭祀公業共有法人所有之差異性;其次過去設立之祭祀公業法人格土地權屬之分析,另規範過去所設立祭祀公業財團法人(宗祠財團法人)之爭議問題;第四章以派下現員之清理、派下權與房份配置及祭祀公業之解散,並深入分析祭祀公業權屬爭議問題。 第五章結論與建議:第一、祭祀公業取得派下全員證明書後,依據私法自治原則及法律本質決定其權屬關係為「祭祀公業法人」,以符合下列祭祀公業原設立之宗旨。第二、祭祀公業依過去法令所設立宗祠財團法人,應准其辦理更名登記為祭祀公業法人,以符合祭祀公業之法律本質、設立宗旨及其主體性。第三、派下員之繼受取得應依設立時章程(鬮書)之約定;未約定時,則依宗祧繼承男子繼承為原則,女子繼承為例外。現存之祭祀公業,既係日據時期所設立,自應適用設立當時之繼承習慣。若為解決男女平權繼承的問題,而恣意以現代民法所規定之男女平權繼承概念予以清理,將導致產權複雜化及違背台灣先民設立祭祀公業慎終追遠之宗旨。第四、祭祀公業解散清算分配賸餘財產時應依設立時章程(鬮書)之約定;未約定時,則依舊慣以房份為原則,均分(推定均等)為例外。最後提出後續研究建議,首先,祭祀公業清理被編定公共設施用地者之財產權保障問題;再者,祭祀公業之資產委由「公益信託」管理之可行性;暨派下權之繼受制度如何定位以符合我國舊慣等,這些議題都值得深入研究。而本文期盼祭祀公業清理制度之實施,能明確界定派下員之權屬關係,以落實財產權之保障。 關鍵字:祭祀公業、法人、宗祠財團法人、男女平權繼承、鬮書 / Abstract “Ancestral estate” is a special, independent property of “joint ownership” formed by tradition and custom, whose purpose is to worship clan ancestors. This study intends to research the similarity of the purpose and objective for establishing the ancestral estate juridical person, governed by Article 50 of the “Statue Governing the Ancestral Estate” and the foundation (Ancestral Shrine Foundation), governed by the Civil Code. By focusing on the following issues: (a) Land ownership before and after acquiring corporate personality of ancestral estate; (b) Authority controversy between clan members; and (c) The appropriateness of inheritance equality between men and women, this study tries to analyze the authority structure of ancestral estate from the perspectives of “due sacrifice for the ancestor” and continuation of traditional custom of clan ancestor worshiping. The purpose of this study is to pinpoint the blind spot existing in the past and current governmental policy concerning ancestral estate so that the government may find useful reference in future implementation of relevant systems and enactment of regulations. Chapter two discusses the nature of ancestral estate and its related regulations: the origin, meaning, historic system timeline, rights and obligation of clan members, and the compilation of controversial interpretations, judgments, precedents, and regulation. Further discussion will also be given on the legal nature, reporting of clan member, and the authorities regarding the revocation and dissolution of organization. Chapter three discusses the subject matters of ancestral estate. Firstly, the difference between the juridical persons of ancestral estate is discussed. Secondly, analyze the land ownership of ancestral estate and regulate the issues existing in the ancestral estate foundation (Ancestral Shrine Foundation). Chapter four deals with the sorting out of clan members, share allocation of property ownership, dissolution of ancestral estate, and an in-depth analysis will also be given on the authority controversies. Conclusion and suggestion will be provided in Chapter five: (1) After acquiring staff certificate of ancestral estate, the ancestral estate shall be deemed as a “ancestral estate juridical person” in accordance with the principle of autonomy and legal nature of private law, to meet the following establishing objectives; (2) The ancestral shrine foundation established in accordance with past regulations shall be allowed to change name and registered as ancestral estate juridical person to meet the legal nature, establishing objective and objectivity; and (3) The inheritance acquisition of clan members shall abide by the terms and conditions set forth in the articles (book of property inheritance); in the event of no agreement is made, the principle of male inheritance in accordance with clan pedigree shall govern, with female inheritance as exception. The existing ancestral estate was first established in the Japanese occupation period, which was applicable to the inheritance practices of the time. If, in order to solve the inheritance equality problem between both sexes, the competent authority tries to sort out in an arbitrary way the current issue with the concept of modern Civil Code, it is bound to cause complexity and in violation to the objective of due offerings set forth by Taiwanese ancestors; (4) The ancestral estate shall set up articles (book of property inheritance) governing its dissolution, liquidation, allocation of residual property; if no agreement is made, it shall be based on pedigree as principle, with even distribution (assumed even) as exception. Finally, suggestions will be provided for subsequent studies concerning: Property protection regarding public facilities designated for the sorting out of ancestral estate; The feasibility of “public trust” management of the assets of ancestral estate; and how to position the system of inheritance acquisition in order to meet the national custom and traditional practices. The main purpose of this study is to realize the protection of property by giving a clearly defined authority of clan members through the implementation of sorting out system of ancestral estate. Keywords:Ancestral estate;Juridical person; Ancestral Shrine Foundation ; Equal inheritance of both sexes; book of property inheritance
93

Étude de l’évolution des génomes par duplications, pertes et réarrangements

Tremblay Savard, Olivier 10 1900 (has links)
La duplication est un des évènements évolutifs les plus importants, car elle peut mener à la création de nouvelles fonctions géniques. Durant leur évolution, les génomes sont aussi affectés par des inversions, des translocations (incluant des fusions et fissions de chromosomes), des transpositions et des délétions. L'étude de l'évolution des génomes est importante, notamment pour mieux comprendre les mécanismes biologiques impliqués, les types d'évènements qui sont les plus fréquents et quels étaient les contenus en gènes des espèces ancestrales. Afin d'analyser ces différents aspects de l'évolution des génomes, des algorithmes efficaces doivent être créés pour inférer des génomes ancestraux, des histoires évolutives, des relations d'homologies et pour calculer les distances entre les génomes. Dans cette thèse, quatre projets reliés à l'étude et à l'analyse de l'évolution des génomes sont présentés : 1) Nous proposons deux algorithmes pour résoudre des problèmes reliés à la duplication de génome entier : un qui généralise le problème du genome halving aux pertes de gènes et un qui permet de calculer la double distance avec pertes. 2) Nous présentons une nouvelle méthode pour l'inférence d'histoires évolutives de groupes de gènes orthologues répétés en tandem. 3) Nous proposons une nouvelle approche basée sur la théorie des graphes pour inférer des gènes in-paralogues qui considère simultanément l'information provenant de différentes espèces afin de faire de meilleures prédictions. 4) Nous présentons une étude de l'histoire évolutive des gènes d'ARN de transfert chez 50 souches de Bacillus. / Gene duplication is one of the most important types of events affecting genomes during their evolution because it can create novel gene function. During the evolution process, genomes are also affected by inversions, translocations (including chromosome fusions and fissions), transpositions and deletions. Studying the evolution of genomes is important to get a better understanding of the biological mechanisms involved, which types of events are more frequent than others and what was the gene content in the ancestral species just to name a few. In order to analyze these different aspects of genome evolution, efficient algorithms need to be developed to infer ancestral genomes, evolutionary histories, homology relationships between genes and to compute distances between genomes. In this thesis, four different projects related to the study and analysis of genome evolution are presented: 1) We developed two algorithms to solve problems related to whole genome duplication: one that generalizes the genome halving problem to gene losses, and one that allows to compute the double distance with losses. 2) We developed a new method to infer evolutionary histories of orthologous tandemly arrayed gene clusters. 3) We proposed a new graph-theoretic approach to infer inparalogs that simultaneously considers the information given by multiple species in order to make better inferences of inparalogous gene pairs. 4) We studied the evolutionary history of the tRNA genes of 50 Bacillus strains.
94

Les génomes bactériens, une histoire de transferts de gènes, de recombinaison et de cladogénèse / Bacterial genomes, a tale of gene transfer, recombination and cladogenesis

Lassalle, Florent 26 November 2013 (has links)
Dans les génomes bactériens, les fréquents transferts horizontaux de gènes (HGT) introduisent des innovations génomiques qui peuvent entraîner la diversification des populations bactériennes. À l'inverse, la recombinaison homologue (RH) au sein des populations homogénéise leurs génotypes, et ainsi renforce leur cohésion. Ces processus d'échange génétique, et la fréquence à laquelle ils interviennent au sein et entre les populations, doivent avoir un grand impact sur la cladogénèse bactérienne. Au-delà de la configuration des échanges qui se sont réellement produits entre les bactéries, les traces de RH et de HGT que nous observons dans leurs génomes reflètent les événements qui ont été fixés tout au long de leur histoire. Ce processus de fixation peut être biaisé en ce qui concerne la nature des gènes ou allèles qui ont été introduits. La sélection naturelle peut notamment conduire à la fixation des gènes transférés qui apportent de nouvelles adaptations écologiques. En outre, des biais mécaniques dans le processus de recombinaison lui-même peuvent conduire à la fixation d'allèles non-adaptatifs. Nous avons cherché à caractériser certains de ces processus adaptatifs et non-adaptatifs qui façonnent les génomes bactériens. À cette fin, plusieurs aspects de l'évolution des génomes, comme les variations de leurs répertoires de gènes, de leur architecture et de leur composition en nucléotides ont été examinés à la lumière de leur histoire de transfert et de recombinaison / In bacterial genomes, the frequent horizontal gene transfers (HGT) introduce genomic novelties that can promote the diversification of bacterial populations. In opposition, homologous recombination (HR) within populations homogenizes their genotypes, enforcing their cohesion. These processes of genetic exchange, and their patterns of occurrence among and within lineages, must have a great impact on bacterial cladogenesis. Beyond the pattern of exchanges actually occurring between bacteria, the traces of HR and HGT we observe in their genomes reflect what events were fixed throughout their history. This fixation process can be biased regarding the nature of genes or alleles that were introduced. Notably, natural selection can drive the fixation of transferred genes that bring new ecological adaptations. In addition, some mechanical biases in the recombination process itself may lead to the fixation of non-adaptive alleles. We aimed to characterize such adaptive and non-adaptive processes that are shaping bacterial genomes. To this end, several aspects of genome evolution, such as variations of their gene repertoires, of their architecture and of their nucleotide composition were examined in the light of their history of transfer and recombination
95

Pauline Freedom: Idolatry and the Vietnamese Ancestor Cult

Nguyen, Daniel Xuan-Vu 11 March 2016 (has links)
No description available.
96

A Geochemical Exploration of the Sagehen Volcanic Centre, Truckee-Tahoe Region, California, U.S.A.

Clarke, Christopher Angus Leo 13 June 2012 (has links)
The assemblage of ca. 6–4 Ma volcanic rocks exposed at the Sagehen Research station in the Truckee-Tahoe region of the northern Sierra Nevada, United States, is interpreted to be, within the Ancestral Cascades volcanic arc, a Lassen-type stratovolcano complex. Sagehen is of particular importance because it is one of the few Tertiary arc volcanic centres in California which has not been heavily glaciated during the Pleistocene. The volcanic rocks are variably porphyritic or aphanitic, including abundant plagioclase with clinopyroxene and amphibole. The rocks range from basalt to basaltic-andesite to andesite in composition. Basalts are olivineand clinopyroxene-bearing with minor phenocrysts of plagioclase. The basaltic-andesites are primarily pyroxene bearing while the andesites contain pyroxene-, plagioclase- and hornblende porphyritic phases. Sagehen arc lavas are calc-alkaline and enriched in the large ion lithophile elements and depleted in High Field Strength Elements. The basalts are depleted in Zr and Hf while the andesites are enriched with Zr and Hf relative to the middle rare earth elements. Compared to previously studied Ancestral Cascade arc samples, Sagehen region basalts have lower 143Nd/144Nd isotopic values that do not correspond to proposed mantle-lithosphere mixing lines, while the andesite samples appear to represent the interplay of these two components on a 87Sr/86Sr vs. 143Nd/144Nd. The trace element data and isotopic plots suggest that the melts that produced the basalts are from subduction modified mantle wedge peridotites that ponded near the base of the lithosphere similar to the generation of other subduction related calc-alkaline lavas along convergent continental margins. The andesitic samples appear to be the result of further modification through crustal assimilation as seen in the higher isotopic Sr contents in the andesites and Ce/Smpmn vs. Tb/Ybpmn plots. Finally, the proposed map units from Sylvester & Raines (2007) were found to contain various geochemical facies based on the samples collected indicating that some map units may have to be redefined or sub-divided.
97

Seeking Freedom through Self-Love in Toni Morrison’s A Mercy and Beloved

Walker, Stephanie 26 July 2012 (has links)
Toni Morrison chose to revisit the neo-slave narrative genre twenty-five years after the publication of Beloved with A Mercy in 2008. With these two texts, Morrison offers her readers one story that shows the descent into slavery and one that shows progression towards freedom. The purpose of this thesis is to place Morrison’s two neo-slave narratives, Beloved and A Mercy, next to one another in order to better understand the journey to freedom through self-love. This work examines the concept of self-love and the necessary components—maternal nurturance, ancestral connection, and communal interaction—that must come together to help Morrison’s characters learn to love and see themselves as their “own best thing.” The repercussions that self-love’s absence has for both individual characters and their larger communities is also discussed and illustrated by the struggles of Florens in A Mercy and Sethe in Beloved.
98

Les "phosphate binding protein" : entre import du phosphate et inhibition de la transcription virale / The "Phosphate Binding Protein" : from the phosphate fixation to the inhibition of HIV transcription

Gonzalez, Daniel 23 June 2014 (has links)
Les « phosphate binding protein » (PBP) constituent une famille de protéines présentes de manière ubiquitaire chez les bactéries et plus marginalement chez les Eucaryotes. Impliquées dans l'import du phosphate extracellulaire chez les bactéries, les PBPs présentent un site de fixation du phosphate très bien caractérisé avec, notamment, une liaison hydrogène particulière nommée «low barrier hydrogen bond» (LBHB). Cette LBHB est impliquée dans la discrimination entre le phosphate et des anions proches chez les PBPs. Bien que cette discrimination semble nécessiter une haute conservation du site de fixation du phosphate, dans la nature différentes configurations sont observées. Au cours de ce travail, nous nous sommes intéressés à la PBP d'un organisme pathogène, C.perfringens qui présente un site de fixation alternatif. Avec, entre autre, une perte de la LBHB, cette PBP présente la plus faible capacité de discrimination testée à ce jour. Cette faible capacité de discrimination pourrait être liée au biotope de la bactérie ou bien à un phénomène d'adaptation fonctionnelle. D'autre part, certaines PBPs présentent des propriétés d'inhibition du VIH via l'étape de la transcription virale. Cependant, ces protéines sont particulièrement difficiles à produire en système hétérologue limitant l'étude fonctionnelle. Afin de lever ce verrou technique, nous avons développé une nouvelle méthodologie basée sur la phylogénie en vue de solubiliser notre modèle d'étude (HPBP). Nous avons obtenu un variant soluble de HPBP qui conserve ses activités antivirales permettant de débloquer les études fonctionnelles. / The "phosphate binding protein" constitutes a family of proteins ubiquitously found in Prokaryotes but also more sparsely distributed in Eukaryotes. Involved in phosphate import, PBPs exhibits a well-characterized phosphate binding site with a peculiar hydrogen bond called "low barrier hydrogen bond" (LBHB). This LBHB is involved in the unique discrimination properties of PBPs, capable of discriminating phosphate from other similar anions such as arsenate of sulfate. Albeit this high discriminating property needs a high conservation of the phosphate binding pocket, different configurations are observed in nature. Herein, we have been interested in a PBP from a human pathogen, Clostridium perfringens, which presents an alternative phosphate binding site. Exhibiting a loss of the LBHB, C.perfringens PBP is the least discriminating PBP isolated so far. This weak discrimination property might be related to the environment of C.perfringens or to a functional adaptation of the PBP. On the other hand, PBPs issued from eukaryotic tissues exhibit HIV inhibition properties via a step not yet targeted in current therapies, i.e. the transcription. However, these proteins are difficult to obtain from human tissues and their expression in heterologous system remains impossible. We have developed a new methodology based on phylogeny in order to solubilise our study model, HPBP. Thus, we have obtained a soluble variant of HPBP which conserves the HIV-inhibiting properties. This unique tool both allow to unlock functional studies and lead to a better understanding on how PBPs are capable of inhibiting HIV.
99

Contribuições aos estudos anatômicos de Bromeliaceae (Poales) sob uma perspectiva filogenética / Contribution to the anatomical studies of Bromeliaceae (Poales) under a phylogenetic perspective

Oliveira, Fernanda Maria Cordeiro de 17 March 2017 (has links)
Bromeliaceae ocupa posição basal na Ordem Poales e é considerada monofilética, tendo sinapomorfias morfológicas e moleculares. Atualmente é subdividida em oito subfamílias, sendo Bromelioideae a subfamília com maiores problemas na delimitação genérica. Nesse contexto encontra-se o Complexo Nidularióide, formado pelos gêneros Nidularium Lem., Wittrockia Lindm., Neoregelia L.B.Sm., Canistropsis (Mez) Leme e Edmundoa Leme. A dificuldade na delimitação destes gêneros se dá pelo uso de um grande número de caracteres não exclusivos, que mostram o íntimo relacionamento entre estes gêneros. Embora estes gêneros não sejam monofiléticos, como indicam as filogenias recentes, o Complexo Nidularióide como um todo sempre emerge em um clado, indicando que formam uma unidade taxonômica. Neste contexto, analisamos caracteres morfológicos e anatômicos a fim de estabelecer novas sinapomorfias para o Complexo. Nossos resultados indicam que os principais caracteres morfológicos utilizados na delimitação dos gêneros do complexo Nidularióide são homoplásticos. A maior parte dos caracteres anatômicos também representam homoplasias. No entanto a anatomia foliar mostrou-se útil, provendo novas sinapomorfias para o grupo, tais como a presença de células da ala alongadas nos tricomas, e presença de células epidérmicas adaxiais com paredes levemente espessadas na lâmina foliar. No segundo capítulo, analisamos a anatomia floral de 16 espécies de Bromeliaceae, pertencente a três das oito subfamílias, a fim de estabelecer caracteres anatômicos florais úteis na sistemática do grupo, bem como discuti-los sob uma perspectiva ecológica e filogenética. Uma nova sinapomorfia é proposta para Pitcairnoideae a partir de dados sobre a vascularização do gineceu. No terceiro capítulo, analisamos o desenvolvimento foliar de três espécies de Tillandsia L. (Tillandsoideae). Neste estudo, foi possível registrar a presença de coléteres nas bainhas foliares próximas aos ápices caulinares de T. tricholepis (L.) L. Estas estruturas são responsáveis pela proteção do meristema apical caulinar (MAC) por meio de seu exsudado. Este estudo é o primeiro registro destas estruturas em Bromeliaceae / Bromeliaceae ocupies a basal position in Poales Order and is considered monophyletic by morphological and molecular sinapomorphies. The family is currently subdivided into eight subfamilies. Bromelioideae has major problems in generic delimitation. In this context is included Nidularioid Complex, formed by the genera Nidularium Lem., Wittrockia Lindm., Neoregelia L.B.Sm., Canistropsis (Mez) Leme e Edmundoa Leme. The difficulty to generic delimitation in these genera occurs by the presence of a great number of non-exclusive character, which demonstrates their intimate relashioship. Although recent phylogenies indicate these genera are not monophyletic, Nidularioid Complex always emerges as a clade, which suggests they are a true taxonomic entity. In this context, we analyzed morphological and anatomical characters to establish new sinapomorphies for the Complex. Our results indicates that the main morphological characters used in genera delimitation on Nidularioid Complex are homoplastic. The major part of anatomical characters are also homoplastic. However, leaf anatomy shows to be useful, providing new synapomorphies for this group, such as the presence of trichomes with elongated wing\'s cells and the presence of adaxial epidermal cells with lightly thickened walls on leaf blade. On the second chapter, we analyzed floral anatomy of 16 species of Bromeliaceae, belonging to three out of eight subfamilies, to establish anatomical floral characters useful in the group sistematics, as well as discuss it under an ecological and phylogenetic perspective. A new synapomorphy for Pitcairnoideae is established, by data of gynoicea vascularization. On the third chapter, we analyzed leaf development of three species of Tillandisia L. (Tillandsoideae). In this study, it was possible to describe the presence of colleters in leaf sheath, next to shoot apices of T. tricholepis (L.) L. These structures are responsible by shoot apical meristem\'s (SAM) protection by their secretion. This is the first record of colleters in Bromeliaceae
100

Estudos evolutivos do divisomo, um complexo multiprotéico responsável pela divisão bacteriana / Evolutionary studies of the divisome, a multiprotein complex responsible for bacterial division

Souza, Robson Francisco de 07 November 2007 (has links)
O mecanismo de divisão mais comum entre procariotos é a divisão binária, na qual a célula- mãe reparte seu genoma e conteúdo citoplasmático de forma igual entre duas células filhas. Esse processo é mediado por um complexo protéico especializado, chamado divisoma, composto por cerca de 20 proteínas, que promovem a constrição da parede celular e membrana citoplasmática, formando o septo de divisão. O complexo é organizado em torno do anel Z, uma estrutura em anel composta pela proteína FtsZ, um homólogo de tubulina presente na maioria dos procariotos e em algumas organelas de eucariotos. Partindo de um levantamento detalhado da distribuição dos genes do divisoma em genomas completos de procariotos, aplicamos métodos de máxima verossimilhança para inferência de estados ancestrais e reconstruímos o conteúdo gênico do divisoma no ultimo ancestral comum das bactérias atuais. Estendendo essas análises com a aplicação de métodos filogenéticos, inferimos os eventos responsáveis pelas variações de composição deste complexo, observadas entre os diferentes grupos de bactérias. Nossos resultados mostram que o último ancestral comum de todas as bactérias já possuía a maior parte dos componentes conhecidos do divisoma, sugerindo a existência de uma parede de peptideoglicano e a presença de um aparato molecular tão ou mais complexo que o observado nas linhagens atuais, incluindo a presença de componentes considerados acessórios e de distribuição relativamente restrita, como as proteínas envolvidas na localização do anel Z (sistema Min) e alguns efetores positivos da polimerização de FtsZ. Observamos também que a evolução do complexo não foi muito afetada por eventos de transferência lateral, mas apresenta vários exemplos de perda de genes, em especial em linhagens com genoma reduzido, o que sugere a redundância de vários componentes já presentes no ancestral e a freqüente redução da complexidade, pelo menos dos componentes centrais do divisoma. Episódios de expansão de famílias de componentes do divisoma em linhagens específicas e os mecanismos evolutivos responsáveis pela incorporação de tais variações são discutidos. A caracterização da história evolutiva detalhada do divisoma, aqui apresentada, poderá servir como ponto de partida para novas análises evolutivas e como base para elaboração de experimentos funcionais. / The most common cell division mechanism among prokaryotes is binary fission, where a mother cell partitions its cytoplasm and genome equally among two daughter cells. This process is mediated by a specialized protein complex, known as the divisome, composed of around 20 proteíns, that promotes constriction of the cell wall and cytoplasmic membrane, thus forming the division septa. The complex is organized around the Z-ring, a ring-shaped struture composed by FtsZ, a tubulin homolog present in most prokaryotes and some eukaryotic organelles. After a detailed revision of the distribution of divisome genes among completely sequenced prokaryotic genomes, we applied maximum likelihood methods for the inference of ancestral states and reconstructed the gene content of the divisome in the last common ancestor of all extant bacteria. We then performed phylogeneticanalysis of all cell division genes and inferred the series of events responsible for the observed variations of the complex´s composition among bactérial lineages and their common ancestor. Our results show that the last common ancestor of all bacteria already possessed most of the known divisome components, thus suggesting the existence of a peptidoglycan cell wall and the presence of a molecular apparatus, perhaps more complex than those found in extant bacteria, including the presence of some accessory components with a somewhat restricted distribution, like the proteíns involved in the localization of the Z-ring (Min sistem) and some positive effectors os FtsZ polimerization. We also observed that the complex´s evolution was almost never the subject of horizontasl gene transfer events, but shows several examples of gene loss, specially in lineages displaying clear signs of genome reduction, thus suggesting the redundancy of several components in the ancestral divisome and a certain degree complexity reduction, at least for core components of the divisome. Lineage specific expansion of divisome component and the evolutionary mechanisms behind such processes are discussed. This characterization of the detailed evolutionary history of the divisome might serve as a starting point for new evolutionary analysis and as a basis for the design of functional experiments.

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