Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-09-12T13:40:01Z No. of bitstreams: 1 paulanettosilva.pdf: 1153373 bytes, checksum: 8181d2e647dd4c1e03663657d63a7785 (MD5) / Approved for entry into archive by Diamantino Mayra (mayra.diamantino@ufjf.edu.br) on 2016-09-13T12:59:40Z (GMT) No. of bitstreams: 1 paulanettosilva.pdf: 1153373 bytes, checksum: 8181d2e647dd4c1e03663657d63a7785 (MD5) / Made available in DSpace on 2016-09-13T12:59:40Z (GMT). No. of bitstreams: 1 paulanettosilva.pdf: 1153373 bytes, checksum: 8181d2e647dd4c1e03663657d63a7785 (MD5) Previous issue date: 2011-02-28 / As abelhas Euglossina são importantes polinizadoras de muitas espécies vegetais neotropicais. Os machos dessa tribo se destacam pelo comportamento peculiar de coletar compostos aromáticos. A utilização de compostos aromáticos sintéticos tem permitido a realização de levantamentos da fauna de Euglossina em diferentes regiões do Brasil. Os objetivos desse estudo foram inventariar as espécies de abelhas Euglossina do Parque Estadual do Ibitipoca (PEIB), avaliar diferenças na riqueza e abundância de espécies em duas formações fitofisionômicas (Mata Atlântica e Campo Rupestre) dentro do parque, analisar a sazonalidade e o horário de atividade do grupo nesses ambientes e comparar a atratividade dos compostos aromáticos utilizados. Os dados foram coletados de janeiro a dezembro de 2010. Foram coletados 162 machos de Euglossina pertencentes a três gêneros e oito espécies. A área de Mata Atlântica apresentou maior riqueza e abundância quando comparada a área de Campo Rupestre, sendo os índices de diversidade de H'= 0,65; J'= 0,33 para a primeira área e H'= 0,29; J'= 0,27 para a segunda. A similaridade da composição de espécies das duas áreas amostradas foi baixa (Q.S. = 0,4). A espécie Eulaema nigrita foi a única dominante em ambas as áreas. A umidade relativa foi correlacionada com a abundância de machos apenas na área de ―Campo Rupestre‖. Os machos foram ativos durante todo o dia de coleta, mostrando maior atividade das 13 ás 14 horas. Dentre os compostos utilizados o eucaliptol foi o mais atrativo em termos de abundância (83%). Em relação à diversidade, eugenol e vanilina foram mais atrativos, atraindo quatro e três espécies, respectivamente. O salicilato de metila atraiu apenas indivíduos de Eulaema cingulata, enquanto o acetato de benzila não foi atrativo. Este é o primeiro levantamento de abelhas Euglossina a ser realizado no PEIB, contribuindo para o conhecimento de alguns padrões dessas abelhas em regiões de Campo Rupestre. / Euglossina bees are important pollinators of many neotropical species of plants. Their males show the behavior of collecting aromatic compounds. The use of these compounds has been used to survey Euglossina bees in different regions of Brazil. This work aims to survey Euglossina bees on Parque Estadual do Ibitipoca (PEIB), comparing differences on: species richness and abundance in two physiognomic formations (Atlantic Forest and "Campo Rupestre"), seasonality and hours of activity of bees in these environments; the attractiveness of three aromatics in attracting bees. The study was conducted from January to December 2010. It was collected 162 Euglossina males belonging to three genera and eight species. The forest area had the highest richness and abundance when compared to "Campo Rupestre", (H'=0,65; J'=0,33 for the first area and H'= 0,29; J' = 0,27 for the second one). The similarity of species composition of the two sampled areas was low (QS = 0.4), this is probably due to differences of each physiognomic environment. The species Eulaema nigrita was the dominant secies in both areas. Euglossina bees were more active during the warm months of the year, coinciding with the rainy season. During the dry season, characterized by cloudy days, with high incidence of sawmill, males activity was very low or absent. Temperature was correlated with the abundance of males. The relative humidity was correlated with the abundance of males only in the "Campo Rupestre" area. Males were active throughout the day of collection, showing the highest activity at 1p.m. Among the attractive compounds, eucalyptol was the most attractive in terms of abundance (83%). In relation to diversity, Eugenol and vanillin were more attractive than eucalyptol, attracting four and three species respectively. The methyl salicylate attracted only Eulaema cingulata, while the benzyl acetate was not attractive. This study is the first survey of Euglossina accomplished in PEIB, contributing to the knowledge of some patterns of these bees "Campo Rupestre".

No description available.

No description available.

[Truncated abstract] Removal of consumers through fishing has been shown to influence ecosystem structure and function by changing the biomass and composition of organisms occupying lower trophic levels. The western rock lobster (Panurilus cygnus), an abundant consumer along the temperate west coast of Australia, forms the basis of Australia's largest single species fishery, with catches frequently exceeding 11000 tonnes annually. Despite their high abundance and commercial importance, the diet and trophic role of adult lobster populations in deep-coastal-ecosystems (35-60 m) remains unknown. An understanding of the diet and trophic role of lobsters in these ecosystems is a key component of the assessment of ecosystem effects of the western rock lobster fishery. This study uses gut content and stable isotope analyses to determine the diet and trophic role of lobsters in deep-coastal ecosystems. Dietary analysis indicated adult lobsters in deep-coastal ecosystems were primarily carnivorous with diet reflecting food available on the benthos. Gut content analyses indicate crabs (62 %) and amphipods/isopods (~10 %) are the most important lobster dietary sources. Stable isotope analysis indicates natural diet of lobsters in deep coastal ecosystems is dominated by amphipods/isopods (contributing up to ~50 %) and crabs (to ~75 %), with bivalves/gastropods, red algae and sponges of lesser importance (<10 % of diet each). Diet of lobsters in deep-coastal ecosystems differed from that reported for lobsters inhabiting shallow water ecosystems in this region, reflecting differences in food availability and food choice between these ecosystems. Bait from the fishery was also determined (by stable isotope analyses) to be a significant dietary component of lobsters in deep-coastal ecosystems, contributing between 10 and 80 % of lobster food requirements at some study locations. '...' Given observed effects of organic matter addition in trawl fisheries, and also associated with aquaculture, bait addition is likely to have implications for processes occurring within deep-coastal ecosystems in this region, particularly given its oligotrophic status, most likely by increasing the food available to scavenging species. Removal of lobsters from deep-coastal ecosystems may affect the composition and abundance of lobster prey communities through a reduction in predation pressure. Such effects have been demonstrated for other spiny lobster species. These effects are typically most observable amongst common prey taxa which in other studies have been commonly herbivores. In deep-coastal ecosystems, crabs and amphipods/isopods are the most common prey taxa and most likely to be effected. The ecosystem-impacts of top-down control of non-herbivorous prey species is unknown and constrains the inferences possible from this study. However, the establishment of 'no-take' areas in deep-coastal ecosystems would allow the ecosystem effects of lobster removal to be further assessed in these deep-coastal ecosystems. While data from the current study did not allow the ecosystem effects of lobster removal to be properly assessed, this study provided information regarding the ecology of western rock lobsters in previously unstudied ecosystems.

Xylophagous borers and termites that damage "timber-in-service" cause millions of dollars loss annually throughout mainland Australia. I developed field-based feeding assays with practical relevance to preventative action and remedial treatment of infestations of xylophagous insects in the built environment. Essential to this work is facilitating a high termite risk by maintaining an environment conducive to sustained foraging by termites. Feeding assays conducted in low termite risk areas are unduly prolonged, with inherently erratic termite feeding responses. I successfully developed feeding assays with a high termite risk. To establish whether a physical barrier, retrofitted as a sleeve, could prevent termites from damaging wooden poles, eighty poles were established within Beerburrum State Forest, south-east Queensland, Australia. Poles within sets were interconnected with buried timber to facilitate a high risk to Coptotermes acinaciformis (Froggatt). Mesh sleeves to a depth of 1.5m did not prevent termite damage to the poles. Hoop pine Araucaria cunninghamii Ait. ex D.Don dowels ("dip-sticks") were useful termite monitoring devices on the poles. The depth of foraging by termites has implications for the effectiveness of retrofitted physical and chemical barriers in general. Timber quantity and quality has a pivotal influence on the aggregation of large numbers of feeding termites necessary for baiting work and for producing efficacy data of relevance to the protection of timber-in-service from termites. Seasonal variation in termite foraging was examined. Both C. acinaciformis and Coptotermes lacteus (Froggatt) were more active feeding in summer than in autumn. In general, mass loss followed mean ambient temperature. Suppression of C. acinaciformis feeding responses relative to C. lacteus during summer, particularly during January, is thought to be due to excessive temperatures in the containers attached to infested trees. Termite foraging behaviour and responses to both varying masses of softwood timbers and varying exposure times were investigated. The rate of mass loss was independent of the number of test specimens and appeared linear. Colonies regulate the number of foragers directly according to the mass of susceptible timber available. This phenomenon was exploited throughout the remainder of the thesis. Of particular significance was the considerable intra- and inter-specific variation in susceptibility of softwoods to C. acinaciformis and Mastotermes darwiniensis Froggatt. The sapwoods tested were susceptible, but the heartwoods were generally resistant to damage by termites. These data confirm that termites are discriminating feeders, and some more so than others. A novel methodology, developed to maintain an environment conducive to sustained foraging by termites during field-testing, is presented. Consistency in timber acceptance and susceptibility to termites is considered to be important in determining the frequency of inspection and the success of bait systems. I evaluate the termiticidal activity of the bait toxicants hexaflumuron and chlorfluazuron for the management of the subterranean termite C. acinaciformis in the field. The Sentricon Colony Elimination System with Baitube-devices containing 0.1% and 1% mass/mass (m/m) hexaflumuron bait toxicant in dry wood flour (Recruit) was successful in eliminating field colonies of C. acinaciformis. Similarly, the Exterra Termite Interception and Baiting System with cellulose-acetate powder containing either 0.05% m/m or 0.25% m/m chlorfluazuron (Requiem) eliminated C. acinaciformis. Indicators (including "dip-sticks") used to monitor colony health were reliable. By using C. acinaciformis, which builds mounds (epigeous nests) in northern Australia, I was able to avoid the use of multiple mark-release schemes to verify the effects of the bait toxicant on the termite colonies. Problems with multiple mark-release schemes, used elsewhere in the world, are discussed. I continued the focus on bait quality and placement by evaluating termite responses to two aspects of treated stakes, their attractiveness and their susceptibility. Three experiments were undertaken near Townsville and at Beerburrum, with Coptotermes and Schedorhinotermes. Whilst there were no significant differences in the rate of location and foraging, with respect to treatments, differences in foraging, with respect to C. acinaciformis and Schedorhinotermes seclusus (Hill) were significant. Coptotermes acinaciformis displayed greater foraging site tenacity than S. seclusus at Beerburrum. Increased consumption of bait, due to the addition of a phagostimulant, needs to be observed in the field before an increase in the efficacy of baits in a termite management program can be claimed. In Australia, softwood timbers are extensively used in building construction and are generally highly susceptible to damage by termites. I focussed on field feeding assays to test efficacy of borate-treated softwoods against termites and to define "adequate" protection thresholds. Laboratory and field data reported in the literature were confusing with regard to what constitutes "adequate" protection thresholds. The confusion was compounded by differences in termite species, timber species used and test methodology. Laboratory data indicated a borate loading of 0.5% m/m boric acid equivalent (BAE) would cause > 90% termite mortality and restrict mass loss in test specimens to 5%. Field data generally suggested that borate loadings in excess of 0.5% m/m BAE were required. The main contribution of the work to increasing our understanding of management options for termites in the built environment was in resolving the confusion between laboratory and field data for borate wood-preservatives. These apparently conflicting results were explained by the presence or absence of untreated feeder material in the test environment. In the absence of untreated feeder material, retention of 0.5% BAE provides adequate protection from Coptotermes sp., whereas in the presence of untreated feeder material, increased retentions are required. Furthermore, the retentions required increase with increased amounts of susceptible material. Some termites, Nasutitermes sp. and M. darwiniensis, for example, are borate-tolerant and borate wood-preservatives are not a viable management option. The lack of uniform standards for termite-test methodology and assessment criteria for efficacy across the world is recognized as a difficulty with research into the performance of wood preservatives with termites. The many variables in laboratory and field assays make "prescriptive" standards difficult to recommend. The use of "performance" standards to define efficacy criteria ("adequate" protection) is discussed. Whilst the majority of the work involves subterranean termites ("termites"), I examine the question of how to define a hardwood timber species as "not susceptible" to lyctine beetle damage. Aspects of the biology, behaviour and management of Lyctus brunneus (Stephens) are reviewed and a novel field sampling and testing protocol to establish lyctine susceptibility of timber species is presented. A sound understanding of the biology and behaviour of xylophagous insects in the field is essential to ensure that management options implemented in the built environment have a strong ecological basis. The success of this study in achieving stated aims, the need for further work and the implications for test methodologies in the field with practical relevance to infestations of xylophagous insects in the built environment are discussed.

Xylophagous borers and termites that damage "timber-in-service" cause millions of dollars loss annually throughout mainland Australia. I developed field-based feeding assays with practical relevance to preventative action and remedial treatment of infestations of xylophagous insects in the built environment. Essential to this work is facilitating a high termite risk by maintaining an environment conducive to sustained foraging by termites. Feeding assays conducted in low termite risk areas are unduly prolonged, with inherently erratic termite feeding responses. I successfully developed feeding assays with a high termite risk. To establish whether a physical barrier, retrofitted as a sleeve, could prevent termites from damaging wooden poles, eighty poles were established within Beerburrum State Forest, south-east Queensland, Australia. Poles within sets were interconnected with buried timber to facilitate a high risk to Coptotermes acinaciformis (Froggatt). Mesh sleeves to a depth of 1.5m did not prevent termite damage to the poles. Hoop pine Araucaria cunninghamii Ait. ex D.Don dowels ("dip-sticks") were useful termite monitoring devices on the poles. The depth of foraging by termites has implications for the effectiveness of retrofitted physical and chemical barriers in general. Timber quantity and quality has a pivotal influence on the aggregation of large numbers of feeding termites necessary for baiting work and for producing efficacy data of relevance to the protection of timber-in-service from termites. Seasonal variation in termite foraging was examined. Both C. acinaciformis and Coptotermes lacteus (Froggatt) were more active feeding in summer than in autumn. In general, mass loss followed mean ambient temperature. Suppression of C. acinaciformis feeding responses relative to C. lacteus during summer, particularly during January, is thought to be due to excessive temperatures in the containers attached to infested trees. Termite foraging behaviour and responses to both varying masses of softwood timbers and varying exposure times were investigated. The rate of mass loss was independent of the number of test specimens and appeared linear. Colonies regulate the number of foragers directly according to the mass of susceptible timber available. This phenomenon was exploited throughout the remainder of the thesis. Of particular significance was the considerable intra- and inter-specific variation in susceptibility of softwoods to C. acinaciformis and Mastotermes darwiniensis Froggatt. The sapwoods tested were susceptible, but the heartwoods were generally resistant to damage by termites. These data confirm that termites are discriminating feeders, and some more so than others. A novel methodology, developed to maintain an environment conducive to sustained foraging by termites during field-testing, is presented. Consistency in timber acceptance and susceptibility to termites is considered to be important in determining the frequency of inspection and the success of bait systems. I evaluate the termiticidal activity of the bait toxicants hexaflumuron and chlorfluazuron for the management of the subterranean termite C. acinaciformis in the field. The Sentricon Colony Elimination System with Baitube-devices containing 0.1% and 1% mass/mass (m/m) hexaflumuron bait toxicant in dry wood flour (Recruit) was successful in eliminating field colonies of C. acinaciformis. Similarly, the Exterra Termite Interception and Baiting System with cellulose-acetate powder containing either 0.05% m/m or 0.25% m/m chlorfluazuron (Requiem) eliminated C. acinaciformis. Indicators (including "dip-sticks") used to monitor colony health were reliable. By using C. acinaciformis, which builds mounds (epigeous nests) in northern Australia, I was able to avoid the use of multiple mark-release schemes to verify the effects of the bait toxicant on the termite colonies. Problems with multiple mark-release schemes, used elsewhere in the world, are discussed. I continued the focus on bait quality and placement by evaluating termite responses to two aspects of treated stakes, their attractiveness and their susceptibility. Three experiments were undertaken near Townsville and at Beerburrum, with Coptotermes and Schedorhinotermes. Whilst there were no significant differences in the rate of location and foraging, with respect to treatments, differences in foraging, with respect to C. acinaciformis and Schedorhinotermes seclusus (Hill) were significant. Coptotermes acinaciformis displayed greater foraging site tenacity than S. seclusus at Beerburrum. Increased consumption of bait, due to the addition of a phagostimulant, needs to be observed in the field before an increase in the efficacy of baits in a termite management program can be claimed. In Australia, softwood timbers are extensively used in building construction and are generally highly susceptible to damage by termites. I focussed on field feeding assays to test efficacy of borate-treated softwoods against termites and to define "adequate" protection thresholds. Laboratory and field data reported in the literature were confusing with regard to what constitutes "adequate" protection thresholds. The confusion was compounded by differences in termite species, timber species used and test methodology. Laboratory data indicated a borate loading of 0.5% m/m boric acid equivalent (BAE) would cause > 90% termite mortality and restrict mass loss in test specimens to 5%. Field data generally suggested that borate loadings in excess of 0.5% m/m BAE were required. The main contribution of the work to increasing our understanding of management options for termites in the built environment was in resolving the confusion between laboratory and field data for borate wood-preservatives. These apparently conflicting results were explained by the presence or absence of untreated feeder material in the test environment. In the absence of untreated feeder material, retention of 0.5% BAE provides adequate protection from Coptotermes sp., whereas in the presence of untreated feeder material, increased retentions are required. Furthermore, the retentions required increase with increased amounts of susceptible material. Some termites, Nasutitermes sp. and M. darwiniensis, for example, are borate-tolerant and borate wood-preservatives are not a viable management option. The lack of uniform standards for termite-test methodology and assessment criteria for efficacy across the world is recognized as a difficulty with research into the performance of wood preservatives with termites. The many variables in laboratory and field assays make "prescriptive" standards difficult to recommend. The use of "performance" standards to define efficacy criteria ("adequate" protection) is discussed. Whilst the majority of the work involves subterranean termites ("termites"), I examine the question of how to define a hardwood timber species as "not susceptible" to lyctine beetle damage. Aspects of the biology, behaviour and management of Lyctus brunneus (Stephens) are reviewed and a novel field sampling and testing protocol to establish lyctine susceptibility of timber species is presented. A sound understanding of the biology and behaviour of xylophagous insects in the field is essential to ensure that management options implemented in the built environment have a strong ecological basis. The success of this study in achieving stated aims, the need for further work and the implications for test methodologies in the field with practical relevance to infestations of xylophagous insects in the built environment are discussed.

Xylophagous borers and termites that damage "timber-in-service" cause millions of dollars loss annually throughout mainland Australia. I developed field-based feeding assays with practical relevance to preventative action and remedial treatment of infestations of xylophagous insects in the built environment. Essential to this work is facilitating a high termite risk by maintaining an environment conducive to sustained foraging by termites. Feeding assays conducted in low termite risk areas are unduly prolonged, with inherently erratic termite feeding responses. I successfully developed feeding assays with a high termite risk. To establish whether a physical barrier, retrofitted as a sleeve, could prevent termites from damaging wooden poles, eighty poles were established within Beerburrum State Forest, south-east Queensland, Australia. Poles within sets were interconnected with buried timber to facilitate a high risk to Coptotermes acinaciformis (Froggatt). Mesh sleeves to a depth of 1.5m did not prevent termite damage to the poles. Hoop pine Araucaria cunninghamii Ait. ex D.Don dowels ("dip-sticks") were useful termite monitoring devices on the poles. The depth of foraging by termites has implications for the effectiveness of retrofitted physical and chemical barriers in general. Timber quantity and quality has a pivotal influence on the aggregation of large numbers of feeding termites necessary for baiting work and for producing efficacy data of relevance to the protection of timber-in-service from termites. Seasonal variation in termite foraging was examined. Both C. acinaciformis and Coptotermes lacteus (Froggatt) were more active feeding in summer than in autumn. In general, mass loss followed mean ambient temperature. Suppression of C. acinaciformis feeding responses relative to C. lacteus during summer, particularly during January, is thought to be due to excessive temperatures in the containers attached to infested trees. Termite foraging behaviour and responses to both varying masses of softwood timbers and varying exposure times were investigated. The rate of mass loss was independent of the number of test specimens and appeared linear. Colonies regulate the number of foragers directly according to the mass of susceptible timber available. This phenomenon was exploited throughout the remainder of the thesis. Of particular significance was the considerable intra- and inter-specific variation in susceptibility of softwoods to C. acinaciformis and Mastotermes darwiniensis Froggatt. The sapwoods tested were susceptible, but the heartwoods were generally resistant to damage by termites. These data confirm that termites are discriminating feeders, and some more so than others. A novel methodology, developed to maintain an environment conducive to sustained foraging by termites during field-testing, is presented. Consistency in timber acceptance and susceptibility to termites is considered to be important in determining the frequency of inspection and the success of bait systems. I evaluate the termiticidal activity of the bait toxicants hexaflumuron and chlorfluazuron for the management of the subterranean termite C. acinaciformis in the field. The Sentricon Colony Elimination System with Baitube-devices containing 0.1% and 1% mass/mass (m/m) hexaflumuron bait toxicant in dry wood flour (Recruit) was successful in eliminating field colonies of C. acinaciformis. Similarly, the Exterra Termite Interception and Baiting System with cellulose-acetate powder containing either 0.05% m/m or 0.25% m/m chlorfluazuron (Requiem) eliminated C. acinaciformis. Indicators (including "dip-sticks") used to monitor colony health were reliable. By using C. acinaciformis, which builds mounds (epigeous nests) in northern Australia, I was able to avoid the use of multiple mark-release schemes to verify the effects of the bait toxicant on the termite colonies. Problems with multiple mark-release schemes, used elsewhere in the world, are discussed. I continued the focus on bait quality and placement by evaluating termite responses to two aspects of treated stakes, their attractiveness and their susceptibility. Three experiments were undertaken near Townsville and at Beerburrum, with Coptotermes and Schedorhinotermes. Whilst there were no significant differences in the rate of location and foraging, with respect to treatments, differences in foraging, with respect to C. acinaciformis and Schedorhinotermes seclusus (Hill) were significant. Coptotermes acinaciformis displayed greater foraging site tenacity than S. seclusus at Beerburrum. Increased consumption of bait, due to the addition of a phagostimulant, needs to be observed in the field before an increase in the efficacy of baits in a termite management program can be claimed. In Australia, softwood timbers are extensively used in building construction and are generally highly susceptible to damage by termites. I focussed on field feeding assays to test efficacy of borate-treated softwoods against termites and to define "adequate" protection thresholds. Laboratory and field data reported in the literature were confusing with regard to what constitutes "adequate" protection thresholds. The confusion was compounded by differences in termite species, timber species used and test methodology. Laboratory data indicated a borate loading of 0.5% m/m boric acid equivalent (BAE) would cause > 90% termite mortality and restrict mass loss in test specimens to 5%. Field data generally suggested that borate loadings in excess of 0.5% m/m BAE were required. The main contribution of the work to increasing our understanding of management options for termites in the built environment was in resolving the confusion between laboratory and field data for borate wood-preservatives. These apparently conflicting results were explained by the presence or absence of untreated feeder material in the test environment. In the absence of untreated feeder material, retention of 0.5% BAE provides adequate protection from Coptotermes sp., whereas in the presence of untreated feeder material, increased retentions are required. Furthermore, the retentions required increase with increased amounts of susceptible material. Some termites, Nasutitermes sp. and M. darwiniensis, for example, are borate-tolerant and borate wood-preservatives are not a viable management option. The lack of uniform standards for termite-test methodology and assessment criteria for efficacy across the world is recognized as a difficulty with research into the performance of wood preservatives with termites. The many variables in laboratory and field assays make "prescriptive" standards difficult to recommend. The use of "performance" standards to define efficacy criteria ("adequate" protection) is discussed. Whilst the majority of the work involves subterranean termites ("termites"), I examine the question of how to define a hardwood timber species as "not susceptible" to lyctine beetle damage. Aspects of the biology, behaviour and management of Lyctus brunneus (Stephens) are reviewed and a novel field sampling and testing protocol to establish lyctine susceptibility of timber species is presented. A sound understanding of the biology and behaviour of xylophagous insects in the field is essential to ensure that management options implemented in the built environment have a strong ecological basis. The success of this study in achieving stated aims, the need for further work and the implications for test methodologies in the field with practical relevance to infestations of xylophagous insects in the built environment are discussed.

Xylophagous borers and termites that damage "timber-in-service" cause millions of dollars loss annually throughout mainland Australia. I developed field-based feeding assays with practical relevance to preventative action and remedial treatment of infestations of xylophagous insects in the built environment. Essential to this work is facilitating a high termite risk by maintaining an environment conducive to sustained foraging by termites. Feeding assays conducted in low termite risk areas are unduly prolonged, with inherently erratic termite feeding responses. I successfully developed feeding assays with a high termite risk. To establish whether a physical barrier, retrofitted as a sleeve, could prevent termites from damaging wooden poles, eighty poles were established within Beerburrum State Forest, south-east Queensland, Australia. Poles within sets were interconnected with buried timber to facilitate a high risk to Coptotermes acinaciformis (Froggatt). Mesh sleeves to a depth of 1.5m did not prevent termite damage to the poles. Hoop pine Araucaria cunninghamii Ait. ex D.Don dowels ("dip-sticks") were useful termite monitoring devices on the poles. The depth of foraging by termites has implications for the effectiveness of retrofitted physical and chemical barriers in general. Timber quantity and quality has a pivotal influence on the aggregation of large numbers of feeding termites necessary for baiting work and for producing efficacy data of relevance to the protection of timber-in-service from termites. Seasonal variation in termite foraging was examined. Both C. acinaciformis and Coptotermes lacteus (Froggatt) were more active feeding in summer than in autumn. In general, mass loss followed mean ambient temperature. Suppression of C. acinaciformis feeding responses relative to C. lacteus during summer, particularly during January, is thought to be due to excessive temperatures in the containers attached to infested trees. Termite foraging behaviour and responses to both varying masses of softwood timbers and varying exposure times were investigated. The rate of mass loss was independent of the number of test specimens and appeared linear. Colonies regulate the number of foragers directly according to the mass of susceptible timber available. This phenomenon was exploited throughout the remainder of the thesis. Of particular significance was the considerable intra- and inter-specific variation in susceptibility of softwoods to C. acinaciformis and Mastotermes darwiniensis Froggatt. The sapwoods tested were susceptible, but the heartwoods were generally resistant to damage by termites. These data confirm that termites are discriminating feeders, and some more so than others. A novel methodology, developed to maintain an environment conducive to sustained foraging by termites during field-testing, is presented. Consistency in timber acceptance and susceptibility to termites is considered to be important in determining the frequency of inspection and the success of bait systems. I evaluate the termiticidal activity of the bait toxicants hexaflumuron and chlorfluazuron for the management of the subterranean termite C. acinaciformis in the field. The Sentricon Colony Elimination System with Baitube-devices containing 0.1% and 1% mass/mass (m/m) hexaflumuron bait toxicant in dry wood flour (Recruit) was successful in eliminating field colonies of C. acinaciformis. Similarly, the Exterra Termite Interception and Baiting System with cellulose-acetate powder containing either 0.05% m/m or 0.25% m/m chlorfluazuron (Requiem) eliminated C. acinaciformis. Indicators (including "dip-sticks") used to monitor colony health were reliable. By using C. acinaciformis, which builds mounds (epigeous nests) in northern Australia, I was able to avoid the use of multiple mark-release schemes to verify the effects of the bait toxicant on the termite colonies. Problems with multiple mark-release schemes, used elsewhere in the world, are discussed. I continued the focus on bait quality and placement by evaluating termite responses to two aspects of treated stakes, their attractiveness and their susceptibility. Three experiments were undertaken near Townsville and at Beerburrum, with Coptotermes and Schedorhinotermes. Whilst there were no significant differences in the rate of location and foraging, with respect to treatments, differences in foraging, with respect to C. acinaciformis and Schedorhinotermes seclusus (Hill) were significant. Coptotermes acinaciformis displayed greater foraging site tenacity than S. seclusus at Beerburrum. Increased consumption of bait, due to the addition of a phagostimulant, needs to be observed in the field before an increase in the efficacy of baits in a termite management program can be claimed. In Australia, softwood timbers are extensively used in building construction and are generally highly susceptible to damage by termites. I focussed on field feeding assays to test efficacy of borate-treated softwoods against termites and to define "adequate" protection thresholds. Laboratory and field data reported in the literature were confusing with regard to what constitutes "adequate" protection thresholds. The confusion was compounded by differences in termite species, timber species used and test methodology. Laboratory data indicated a borate loading of 0.5% m/m boric acid equivalent (BAE) would cause > 90% termite mortality and restrict mass loss in test specimens to 5%. Field data generally suggested that borate loadings in excess of 0.5% m/m BAE were required. The main contribution of the work to increasing our understanding of management options for termites in the built environment was in resolving the confusion between laboratory and field data for borate wood-preservatives. These apparently conflicting results were explained by the presence or absence of untreated feeder material in the test environment. In the absence of untreated feeder material, retention of 0.5% BAE provides adequate protection from Coptotermes sp., whereas in the presence of untreated feeder material, increased retentions are required. Furthermore, the retentions required increase with increased amounts of susceptible material. Some termites, Nasutitermes sp. and M. darwiniensis, for example, are borate-tolerant and borate wood-preservatives are not a viable management option. The lack of uniform standards for termite-test methodology and assessment criteria for efficacy across the world is recognized as a difficulty with research into the performance of wood preservatives with termites. The many variables in laboratory and field assays make "prescriptive" standards difficult to recommend. The use of "performance" standards to define efficacy criteria ("adequate" protection) is discussed. Whilst the majority of the work involves subterranean termites ("termites"), I examine the question of how to define a hardwood timber species as "not susceptible" to lyctine beetle damage. Aspects of the biology, behaviour and management of Lyctus brunneus (Stephens) are reviewed and a novel field sampling and testing protocol to establish lyctine susceptibility of timber species is presented. A sound understanding of the biology and behaviour of xylophagous insects in the field is essential to ensure that management options implemented in the built environment have a strong ecological basis. The success of this study in achieving stated aims, the need for further work and the implications for test methodologies in the field with practical relevance to infestations of xylophagous insects in the built environment are discussed.

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