Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references (leaves 81-90). Also available on the Internet.

Thesis (doctoral)--Universiteit van Amsterdam, 1991. / Summary in Dutch. Includes bibliographical references (p. 94-102).

Thesis (Ph. D.)--University of Florida, 1981. / Description based on print version record. Typescript. Vita. Includes bibliographical references (leaves 328-345).

142f. / Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-05-07T18:27:22Z No. of bitstreams: 1 Dissertacao_Vera Montano.pdf: 625465 bytes, checksum: 61bd8e82a27a35f7944d2e5d658cdc15 (MD5) / Approved for entry into archive by Rodrigo Meirelles(rodrigomei@ufba.br) on 2013-05-08T12:16:31Z (GMT) No. of bitstreams: 1 Dissertacao_Vera Montano.pdf: 625465 bytes, checksum: 61bd8e82a27a35f7944d2e5d658cdc15 (MD5) / Made available in DSpace on 2013-05-08T12:16:31Z (GMT). No. of bitstreams: 1 Dissertacao_Vera Montano.pdf: 625465 bytes, checksum: 61bd8e82a27a35f7944d2e5d658cdc15 (MD5) Previous issue date: 2007 / Esta Dissertação de mestrado se propõe a contribuir para a elucidação de como a qualidade da prática docente na Educação Infantil está na dependência do domínio das experiências psicocorporais por parte do educador, uma vez que é da sua responsabilidade a organização do meio pedagógico propício à aprendizagem e ao desenvolvimento da criança. Compreender, vivenciar e dominar sua corporeidade, por parte do educador contribui para a organização das estruturas psicocorporais em desenvolvimento na criança. Re-significar a vivência corporal, por parte do educador infantil, o capacita para uma prática estruturante de si mesmo e , conseqüentemente, da criança no que se refere ao desenvolvimento de suas estruturas psicocorporais. As teorias sobre a corporeidade e o psiquismo, que embasam este estudo, provêm da Psicossomática, da Psicomotricidade, da Psicomotricidade Relacional, da Bioenergética, da Ludicidade, aplicadas à Educação Infantil. Esta Dissertação propõe que na formação do educador, principalmente do educador infantil, sejam incluídas vivências que o capacitem a melhor conhecer sua corporeidade e a disponibilizá-la na relação pedagógica com as crianças, seus educandos. / Salvador

Submitted by Glauber Assunção Moreira (glauber.a.moreira@gmail.com) on 2018-08-31T15:49:51Z No. of bitstreams: 1 DISSERTAÇAO final.pdf: 3565325 bytes, checksum: 262d5bb91a5b3159f0ee2507169dbf33 (MD5) / Approved for entry into archive by Ednaide Gondim Magalhães (ednaide@ufba.br) on 2018-09-11T13:15:40Z (GMT) No. of bitstreams: 1 DISSERTAÇAO final.pdf: 3565325 bytes, checksum: 262d5bb91a5b3159f0ee2507169dbf33 (MD5) / Made available in DSpace on 2018-09-11T13:15:40Z (GMT). No. of bitstreams: 1 DISSERTAÇAO final.pdf: 3565325 bytes, checksum: 262d5bb91a5b3159f0ee2507169dbf33 (MD5) / A pesquisa analisou um processo criativo grupal que propõe a introdução de exercícios e teorias da Psicologia Bioenergética em uma proposta de treinamento de palhaço. Para a consecução desse objetivo realizamos o laboratório “II Encontro Oficina: Palhaço e Psicologia Bioenergética”. O pressuposto deste trabalho é que certos princípios da Psicologia Bioenergética podem servir para o desenvolvimento da criatividade na arte do palhaço. Trata-se de um estudo de caso, onde foram utilizadas técnicas como entrevistas, vídeos, fotografia e diário de campo. A análise teve como objetivo formular relações possíveis entre as duas áreas e examinar, junto à percepção dos participantes, essa possibilidade de exploração do palhaço. Considerou-se finalmente que, nas artes do espetáculo, o resultado cênico desse processo pode contribuir para evidenciar uma complementaridade e integração de ferramentas artísticas e terapêuticas que aprofundam e expandem a capacidade criativa dos exploradores dessa arte. / The research examines a group creative process which proposes the introduction of exercises and theories of Bioenergetics Psychology in a clown training proposal. To achieve this objective we performed the laboratory: "II Meeting Workshop: Clown and Bioenergetics Psychology". The assumption of this work is that certain principles of Bioenergetic Psychology can serve for the development of creativity in the art of clowning. This is a case study, which used techniques such as interviews, videos, photography and field journal. The analysis aims to make possible relations between the two areas and examine, with the participants' perceptions, that possibility of clown exploration. In performing arts, scenic results of this process can contribute to highlight a complementarity and integration of artistic and therapeutic tools that deepen and expand the creative capacity of the explorers of this art.

abstract: The basic scheme for photosynthesis suggests the two photosystems existing in parity with one another. However, cyanobacteria typically maintain significantly more photosystem I (PSI) than photosystem II (PSII) complexes. I set out to evaluate this disparity through development and analysis of multiple mutants of the genetically tractable cyanobacterium Synechocystis sp. PCC 6803 that exhibit a range of expression levels of the main proteins present in PSI (Chapter 2). One hypothesis was that the higher abundance of PSI in this organism is used to enable more cyclic electron flow (CEF) around PSI to contribute to greater ATP synthesis. Results of this study show that indeed CEF is enhanced by the high amount of PSI present in WT. On the other hand, mutants with less PSI and less cyclic electron flow appeared able to maintain healthy levels of ATP synthesis through other compensatory mechanisms. Reduction in PSI abundance is naturally associated with reduced chlorophyll content, and mutants with less PSI showed greater primary productivity as light intensity increased due to increased light penetration in the cultures. Another question addressed in this research project involved the effect of deletion of flavoprotein 3 (an electron sink for PSI-generated electrons) from mutant strains that produce and secrete a fatty acid (Chapter 3). Removing Flv3 increased fatty acid production, most likely due to increased abundance of reducing equivalents that are key to fatty acid biosynthesis. Additional components of my dissertation research included examination of alkane biosynthesis in Synechocystis (Chapter 4), and effects of attempting to overexpress fibrillin genes for enhancement of stored compounds (Chapter 5). Synechocystis is an excellent platform for metabolic engineering studies with its photosynthetic capability and ease of genetic alteration, and the presented research sheds light on multiple aspects of its fundamental biology. / Dissertation/Thesis / Doctoral Dissertation Biology 2017

Les mitochondries jouent un rôle primordial dans la survie et la mort cellulaire car elles gouvernent à la fois le métabolisme énergétique et les voies apoptotiques. Un dysfonctionnement mitochondrial dans les neurones peut donc conduire à la neurodégénérescence ou à une neuropathologie. Notre objectif a été d'étudier la régulation de la fonction mitochondriale, en particulier la bioénergétique, pour contribuer à l'amélioration des connaissances actuelles sur les mitochondries. Nos résultats montrent que: i) les neurostéroïdes améliorent la bioénergétique mitochondriale en stimulant la respiration cellulaire en condition normale; ii) les neurostéroïdes réduisent les déficits bioénergétiques observés dans la maladie d'Alzheimer; iii) l'horloge circadienne développe une régulation réciproque avec la bioénergétique et la dynamique mitochondriales. Les résultats de cette thèse ouvrent des perspectives intéressantes pour l'élaboration de stratégies régulatrices de l'homéostasie métabolique chez le sujet sain et chez le patient atteint d'une pathologie due à un dysfonctionnement mitochondrial et/ou une altération des rythmes biologiques. / Mitochondria play a paramount role in cell survival and death because they are orchestrating both energy metabolism and apoptotic pathways, while impaired mitochondrial function leads inevitably to disease, especially neurodegeneration. The purpose of the present thesis was therefore to deepen our understanding of the regulation of mitochondrial function, with a focus on mitochondrial bioenergetics and dynamics. Our key findings were that: i) neurosteroids represent promising molecules which are able to increase mitochondrial bioenergetics via enhancement of mitochondrial respiration in healthy condition; ii) neurosteroids are able to alleviate Alzheimer’s disease-related bioenergetic deficits; iii) the circadian clock is able to regulate mitochondrial bioenergetics and dynamics, and vice versa. Collectively, our results contribute to a better understanding of how mitochondria function, and could have multiple implications with regard to the regulation of metabolic homeostasis in health and disease states associated with mitochondrial impairments and/or circadian disruption.

Diabetes mellitus affects more than 382 million people worldwide and an estimated 30-50% of patients develop some form of neuropathy. Patients typically present with sensory symptoms including hypersensitivity/pain and/or loss of somatosensation. In diabetic neuropathy, the longest nerves of the peripheral nervous system (PNS) show the worst pathology and symptoms are typically felt in the distal extremities. The cause of this apparent length-dependent pathology remains unknown. Through comprehensive integration of untargeted proteomic and metabolomic analyses of the PNS in the streptozotocin rat model of diabetes, we showed that bioenergetic pathways were more dysfunctional in the distal sciatic nerve (SN) than the lumbar 4/5 dorsal root ganglia (DRG) and cranial trigeminal ganglia (TG). Whilst glucose levels increased in all tissues in diabetes, there was extensive upregulation of proteins involved in mitochondrial oxidative phosphorylation in the distal SN compared to healthy age/weight-matched controls which was not evident in the proximal DRG or TG. There were significant changes in lipid metabolites in the SN, a phenomenon which is less apparent in the DRG and not evident in the TG. We investigated the therapeutic potential of copper chelation with triethylenetetramine to reverse such changes and whilst copper chelation prevented nerve conduction velocity deficits, it did not alter aberrant nerve metabolism. To further understand the functional deficits in diabetic neuropathy, we performed in vivo microelectrode recordings from the TG and the thalamic ventral posteromedial (VPM) nucleus in control and diabetic rats in response to precise whisker stimulation. Recordings from the TG showed that the tuning of the primary afferents to graded stimuli is preserved in diabetes. Furthermore, we found that neurons within the VPM showed increased spontaneous activity in diabetes, but maintained tuning to graded whisker stimulation in their evoked firing rate. Thus, the cranial TG appear to be relatively unaffected by diabetes at a biochemical or physiological level, but diabetes may lead to pathophysiological changes within the thalamus which could alter somatosensory processing. Despite a global metabolic insult in diabetes, the molecular consequences are not consistent throughout the nervous system. We show that metabolic dysfunction occurs specifically in regions known to be more affected in neuropathy. Due to such a focal dysfunction, aberrant oxidative phosphorylation in the sciatic nerve may be a key driver to the distal pathogenesis of diabetic neuropathy.

Blood flow distribution during forced and voluntary diving in ducks, and the energetic cost of diving was investigated. It has been suggested that in order for the leg muscles to generate enough power for ducks to dive, blood flow to those tissues must be maintained. A technique to determine blood flow distribution which could be used during voluntary diving was first developed and tested during forced laboratory dives of ducks. This technique was then used to determine the blood flow distribution during voluntary diving. Regional blood flow distribution was visualized by utilizing a radioactive tracer technique (macro aggregated albumin labelled with ⁹⁹ⅿ technetium). The tracer when injected into an animal is trapped and held by capillaries. During forced dives in dabbling (Anas platyrhynchos) and diving (Aythya affinis) ducks the blood flow distribution was found to be restricted to the thoracic and head areas. Whereas during a voluntary dive in A. affinis blood flow distribution was shown to be preferentially directed towards three tissue areas, the heart, brain, and active leg muscles. The work required to dive was determined from the measurement of subsurface drag forces and buoyancy in A. affinis. Subsurface drag increased as a nonlinear function of swimming velocity. At a velocity of 1 m•s⁻¹, the drag force was approximately 1.067 N. The average measured buoyant force of 11 ducks was 0.953 N. The calculated mechanical work done by ducks during a 14.4 s unrestrained dive was 9.34 J. The power output during voluntary was estimated to be 0.751 W (0.0374 ml 0₂•s⁻¹). During diving buoyancy is clearly the dominant force (8.8 J) against which ducks have to work while drag (0.54 J) adds little (~6%) to the energetic cost of diving. / Science, Faculty of / Zoology, Department of / Graduate

A ribonucleoproteína heterogênea nuclear K (hnRNP K) é uma proteína envolvida em processos de expressão gênica e tem sido proposta como ligante de RNAs mensageiros mitocondriais. Apesar de ser considerada um marcador de pior prognóstico no câncer de cabeça e pescoço, o papel da hnRNP K nesta doença ainda é pouco conhecido. O objetivo deste trabalho foi estudar o envolvimento da hnRNP K na mitocôndria com ênfase na bioenergética e na identificação de novos potenciais ligantes de hnRNP K. As linhagens celulares utilizadas foram de carcinoma de cabeça e pescoço (HN13 e CAL 27) com silenciamento de RNA para hnRNP K e células HEK293 com super-expressão de hnRNP K. O efeito do acúmulo celular da hnRNP K na cadeia transportadora de elétrons mitocondrial foi avaliado por meio da atividade dos complexos mitocondriais I, II e V em células HN13. A redução do nível de hnRNP K usando RNA de interferência promoveu uma diminuição da atividade dos complexos nas células HN13, indicando o envolvimento da proteína na eficiência do transporte de elétrons na cadeia respiratória mitocondrial. Células HEK293 com super-expressão da hnRNP K (HEK293/hnRNP K) e as linhagens HN13 e CAL 27 com silenciamento e redução estável de hnRNP K foram utilizadas para determinar o papel de hnRNP K no potencial de membrana mitocondrial, níveis de ATP, produção de lactato e consumo de oxigênio. Células HEK293/hnRNP K comparadas ao controle apresentaram maior nível de ATP, menor potencial de membrana mitocondrial, menor consumo de oxigênio e maior produção de lactato. As células HN13 com redução da hnRNP K apresentaram níveis mais baixos de ATP, com menor liberação de lactato para o meio extracelular e maior consumo de oxigênio. Esses resultados sugerem que o acúmulo da proteína hnRNP K tem ação importante na mitocôndria por alterar o metabolismo bioenergético celular de fosforilação oxidativa para glicólise anaeróbica. A estratégia de co-imunoprecipitação usando anticorpos para hnRNP K, digestão de proteínas com tripsina e cromatografia líquida acoplada a espectrômetria de massa foi usada para encontrar novos potenciais ligantes de hnRNP K. A análise dos dados com o software SEPro identificou 57 proteínas candidatas a ligantes da hnRNP K. Três proteínas foram validadas por co-IP e Western blotting: o fator de transcrição mitocondrial PTCD3, YB1 e PSF. Propomos que a hnRNP K apresenta função na energética mitocondrial, e provavelmente, a sua interação com PTCD3 participa desta função. / Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a protein involved in gene expression processes, which has been proposed to bind mitochondrial mRNAs. Despite it to be considered a prognostic marker in cancer, the hnRNPK role in this disease is unknown. We addressed the involvement of hnRNP K in mitochondria with emphasis on bioenergetics and identification of new potential ligands of hnRNP K. The cell lines used were from head and neck squamous cell carcinoma (HN13 and CAL 27) with RNA silencing for hnRNP K , and HEK293 cells with overexpression of hnRNP K. The effects of cellular accumulation of hnRNP K in mitochondrial electron chain carriers were assessed by the activity of mitochondrial complexes I, II and V in HN13 cells. Reduced levels of hnRNP K using RNA interference promoted a decrease in the activity of the complexes in HN13 cells, indicating the involvement of the protein in the efficiency of the electron transport in mitochondrial respiratory chain. HEK293 cells with overexpression of hnRNP K (HEK293/hnRNP K) and HN13 and CAL 27 cells with silencing and stable reduction of hnRNP K were used to determine the role of hnRNP K in mitochondrial membrane potential, ATP levels, lactate production and oxygen consumption. HEK293/hnRNP K, compared to control cells, showed higher levels of ATP, reduced mitochondrial membrane potential, lower oxygen consumption and higher production of lactate. HN13 cells with reduced hnRNP K had lower ATP levels, with lower release of lactate to the extracellular medium and higher oxygen consumption. These results suggest that accumulation of hnRNP K protein plays a role in mitochondria by changing the cellular energetic metabolism from oxidative phosphorylation to glycolysis. The strategy of co-immunoprecipitation using antibodies for hnRNP K, protein digestion with trypsin, and liquid chromatography, coupled to mass spectrometer, were used to search for new potential ligands of hnRNP K. Data analysis with software SEPro identified 57 candidate proteins binding to hnRNP K. Three proteins were validated by co-IP and Western blotting: the mitochondrial transcription factor PTCD3, YB1, and PSF. We propose that hnRNP K plays a role in the mitochondrial energetics, and probably its interaction with PTCD3 participates in this function.