Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-06-08T13:33:20Z No. of bitstreams: 1 2015 - Samara Macedo Pinto.pdf: 970121 bytes, checksum: f69dfd12be8357633a632969c91a1d3a (MD5) / Made available in DSpace on 2017-06-08T13:33:20Z (GMT). No. of bitstreams: 1 2015 - Samara Macedo Pinto.pdf: 970121 bytes, checksum: f69dfd12be8357633a632969c91a1d3a (MD5) Previous issue date: 2015-05-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Fish communities in developing countries have, in many cases, showed a decrease in the species richness and abundance over time as a result of anthropogenic impacts. We analyzed fish communities of three zones (inner, middle and outer) of the Sepetiba Bay (Southeastern Brazil) using identical sampling methods (bottom trawl) during four different time periods: 1987?1988; 1993?1995; 1998?2001 and 2012?2013. A total of 33,140 fish representing 127 species in 88 genera and 41 families were recorded. Our results revealed highly significant and consistent differences in the fish community structure among the bay zones, and a decreased in the fish richness and abundance over the time period. Changes in fish richness and abundance were related mostly to a sharp decrease that occurred in the inner bay zone, the most impacted bay area, and, to a lesser extent, in the middle zone between the two first (1987?1988 and 1993?1995) and the two latter time periods (1998?2001 and 2012?2013), whereas the outer zone remained comparatively stable over time. Spatial changes in the fish community structure among the bay zones were related to differences in salinity, transparency and depth with this latter variable, together with other factors, acting as a buffer for stabalizing community changes; however, these environmental variables explained little of the variation in the temporal changes. The most important changes over time in abundant species were the numerical decreases of the Ariid Cathorops spixii, the serranid Diplectrum radiale and sciaenid Cynoscion leiarchus, and increases of the sciaenid Micropogonias furnieri and the marine catfish Genidens genidens, with this latter species restricted to the inner and middle zones. This study adds long-term information on gradual fish community changes along spatial gradients of environmental and anthropogenic influences in embayment systems. It is perhaps timely to link conservation and management planning with historical information to protect fish biodiversity in tropical developing countries / As comunidades de peixes em pa?ses em desenvolvimento, em muitos casos, mostram uma diminui??o na riqueza e abund?ncia das esp?cies ao longo do tempo como resultado das evid?ncias dos impactos antropog?nicos. N?s analisamos as comunidades de peixes ao longo de tr?s zonas (interna, central e externa) da Ba?a de Sepetiba (Sudeste do Brasil), utilizando m?todos id?nticos de amostragem (bottom trawl) de peixes durante quatro per?odos temporais diferentes: 1987?1988; 1993?1995; 1998?2001 e 2012?2013. Registrou-se um total de 33.140 peixes representando 127 esp?cies em 88 g?neros e 41 fam?lias. Nossos resultados revelaram diferen?as altamente significativas e consistentes na estrutura da comunidade de peixes entre as zonas da ba?a e uma diminui??o na riqueza de esp?cies e abund?ncia ao longo do per?odo de estudo. As mudan?as na riqueza de esp?cies e abund?ncia foram relacionadas, principalmente, ? uma forte queda que ocorreu na zona interna da ba?a, ?rea esta mais afetada e, em menor medida, na zona central entre os dois primeiros (1987?1988 e 1993?1995) e os ?ltimos dois per?odos temporais (1998?2001 e 2012?2013), enquanto a zona exterior permaneceu relativamente est?vel ao longo do tempo. Mudan?as espaciais na estrutura da comunidade de peixes entre as zonas da ba?a foram relacionadas ?s diferen?as de salinidade, transpar?ncia e profundidade com este ?ltima vari?vel, em conjunto com outros fatores, atuando como um ?buffer? estabilizando mudan?as na comunidade; no entanto, essas vari?veis ambientais explicaram pouco a varia??o nas mudan?as temporais. As mudan?as mais importantes ao longo do tempo em esp?cies abundantes foi a diminui??o num?rica do Ariideo Cathorops spixii, do Serran?deo Diplectrum radiale e do Sciaen?deo Cynoscion leiarchus, e o aumento do Sciaen?deo Micropogonias furnieri e o bagre marinho Genidens genidens, com esta ?ltima esp?cie restrita ?s zonas interna e central. Este estudo acrescenta informa??o de longo prazo sobre mudan?as graduais nas comunidades de peixes ao longo de gradientes espaciais com influ?ncias ambientais e antr?picas no sistema de ba?a. Talvez seja oportuno vincular conserva??o e planejamento de gest?o com informa??es hist?ricas para proteger a biodiversidade de peixes em pa?ses tropicais em desenvolvimento.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-14T19:34:21Z No. of bitstreams: 1 2015 - Veronica Cristina Lopes Menezes.pdf: 1388664 bytes, checksum: eb95b2be98ffd5f3a96140f14377f8f2 (MD5) / Made available in DSpace on 2017-06-14T19:34:21Z (GMT). No. of bitstreams: 1 2015 - Veronica Cristina Lopes Menezes.pdf: 1388664 bytes, checksum: eb95b2be98ffd5f3a96140f14377f8f2 (MD5) Previous issue date: 2015-07-30 / CAPES / The distribution of estrogen receptors in brain structures that are envolved in the hidroelectrolyte balance such as the lamina terminalis (LT), subfornical organ (SFO) and dorsal raphe nucleus (DRN) demonstrated that estradiol can modulate important responses in body fluids. In the literature experimental data support that estrogen can increase the triptofan-hydroxilase type 2 , the main enzyme for the serotonin synthesis. The primary question here is whether or not differences in the baseline or stimulated intake are a function of different levels of circulating gonadal hormones in female ovariectomized rats. Female Wistar intact rats (~230 g) were previously aclimated in metabolic cages during 5 days and ad libitum access to water and hypertonic saline (1.8%) bottles and food. Room temperature was maintained at 22?2 ?C with 12:12 h light-dark cycle (lights off at 19:00). Rats were anesthetized with intraperitoneal injections of a mixture of ketamine (75 mg/kg) and xylazine (5 mg/kg) and then bilaterally ovariohysterectomized. There were four experimental groups: OVX (replaced with corn oil), 2,5 ?g/kg (E2 2,5), 10,0 ?g/kg (E2 10,0) and 40,0 ?g/kg (E2 40,0), daily during seven days, s.c. After 24 h of the surgery the hormonal replacement initiated (estradiol cypionate, EC, Pfizer, Animal Health). We did three experimental protocols: baseline evaluations, sodium depletion and fluid replacement. The estrogen replacement exibitted a dose dependent effect in the following parameters under basal conditions: daily body weight, daily urinary volume and daily food intake. After sodium depletion there were no difference in the urinary volume after 2 and 24 hours of the experiment. But after fluid reposition we observed a dose dependent effect in the ingestive behaviour of water and hypertonic saline intake in sodium depleted and control animals. Our data support that estradiol can alter the natriorexigenic and dipsogenic responses especially after sodium depletion depending of the estrogenic status. / A distribui??o de receptores estrog?nicos em estruturas centrais envolvidas na regula??o da homeostase hidroeletrol?tica como o ?rg?o vasculoso da l?mina terminal, n?cleo subfornicial, n?cleo dorsal da rafe indica que o estradiol pode atuar nessas estruturas em resposta a altera??es nos fluidos corporais. Nosso objetivo foi verificar se a reposi??o hormonal pode influenciar de maneira concentra??o-dependente o status hidroeletrol?tico e neuroend?crino de ratas castradas com reposi??o hormonal em diferentes doses de forma comparativa. Ratas Wistar (~230 g) foram previamente adaptadas, por 5 dias, em gaiolas metab?licas, com acesso ad libitum aos bebedouros volum?tricos de ?gua e salina hipert?nica e ao alimento, sendo mantidas sob ciclo claro-escuro de 12 horas em sala com temperatura controlada em 22??2 ?C. Ao final da adapta??o, as ratas previamente anestesiadas com cetamina (75 mg/kg) e xilazina (5 mg/kg) foram submetidas ? cirurgia de ovariectomia bilateral. Os animais foram divididos em 4 grupos: OVX, reposi??o com ?leo de milho), repostos com ?leo de milho cipionato de estradiol (E2) 2,5 ?g/kg (E2 2,5), 10,0 ?g/kg (E2 10,0) e 40 ?g/kg (E2 40,0). O tratamento de reposi??o foi feito pela via subcut?nea, diariamente durante 7 dias tendo sido iniciado no dia seguinte ? cirurgia. Foram realizados tr?s protocolos experimentais: avalia??o sob condi??es basais, deple??o de ?ons s?dio e reapresenta??o de fluidos. Neste estudo o estradiol apresentou efeito dose dependente nos seguintes par?metros sob condi??es basais: peso corporal di?rio, volume urin?rio di?rio, ingest?o de alimento di?rio. Ap?s deple??o de s?dio n?o houve diferen?a em rela??o ao volume urin?rio de 2 e de 24 horas ap?s o experimento. No entanto ap?s a reapresenta??o dos fluidos houve efeito dose-dependente no comportamento ingestivo de ?gua e de salina hipert?nica tanto nos animais depletados de s?dio quanto nos animais controles.Os dados suportam que o estradiol modula o comportamento ingestivo dos animais sob condi??es basais e ap?s a deple??o de s?dio.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-26T19:35:59Z No. of bitstreams: 1 2016 - David Castor Maxwel de Oliveira.pdf: 6489134 bytes, checksum: 23e02124d5d66aa42a02e4375f4e0862 (MD5) / Made available in DSpace on 2017-06-26T19:35:59Z (GMT). No. of bitstreams: 1 2016 - David Castor Maxwel de Oliveira.pdf: 6489134 bytes, checksum: 23e02124d5d66aa42a02e4375f4e0862 (MD5) Previous issue date: 2016-10-24 / Agroforestry systems (ASF) are potentially characterized as a agroecological crops by the similarity to the forest structure and function which gives more sustainability to the production systems, making them environmentally friendly. This study evaluated an AFS ten years plantation to obtain information about the nitrogen fixing legumes influenced over the soil chemical properties, the palm heart production, the Khaya ivorensis A. Chevalier and Euterpe oleracea Mart. Growth, and finally the common beans cultivation. The AFS was located at Embrapa Agrobiologia Experimental field, at Serop?dica city, Rio de Janeiro state. The statistical was randomized blocks with 5 treatments and 4 replications with 9 m x 9 m plots and total area with 1.620 m2. There were E. oleraceae, planted in a 3 m x 3 m spacing with one plant of K. ivorensis at the plot center. The treatments were different kinds of green manure crops between the lines such as: Acacia angustissima (Mill.) Kuntze; Gliricidia sepium (Jacq.) Steud;. Canavalia ensiformis (L.) DC, nitrogen fertilization with castor cake and spontaneous vegetation. For the soil fertility analyses were collected two samples, one before and other after green manure crops management. K. ivorensis and E. oleraceae biometry, also heart palm production were harmed by inappropriate management on cultivation care lack between post planting and this evaluation lapse. These commercial crops reached larger diameters and superior height at the plot without leguminous crops competition. Green manure crops were efficient in soils nutrients cycling, increasing the most elements content after pruning the leguminous plants and the application of the biomass over the ground. Extract nodules inoculation showed capacity to increase nodulation and nitrogen content in common beans plants, but didn?t increase the grain production. / Os sistemas agroflorestais (SAF) podem ser configurados como cultivos agroecol?gicos, onde a similaridade com as florestas em estrutura e fun??o confere ao sistema uma maior sustentabilidade, tornando-os ambientalmente adequados. Este estudo avaliou um SAF com 10 anos de implanta??o, gerando informa??es sobre quanto as culturas de leguminosas fixadoras de nitrog?nio influenciaram nas propriedades qu?micas do solo e na produ??o do sistema, quantificando a produ??o de palmito, o crescimento de plantas de Khaya ivorensis A. Chevalier e Euterpe oleracea Mart. plantados e o cultivo de feij?o. O experimento estava localizado no Campo Experimental da Embrapa Agrobiologia, no munic?pio de Serop?dica, do Rio de Janeiro. O delineamento experimental adotado foi o de blocos ao acaso com cinco tratamentos e quatro repeti??es, com parcelas de 9 m x 9 m cada, totalizando 1.620 m2. Em todos os tratamentos existiam a?a?s plantados de 3 m x 3 m ao longo de todas as parcelas que tamb?m continham um mogno africano no centro de cada uma. Os tratamentos foram compostos por aduba??es verdes nas entrelinhas dos a?a?s, constitu?dos por Acacia angustissima (Mill.) Kuntze; Gliricidia sepium (Jacq.) Steud.; p aduba??o nitrogenada de cobertura com torta de mamona e o controle com vegeta??o espont?nea. Para avalia??o qu?mica do solo foram feitas duas coletas, uma antes e outra p?s manejo da biomassa vegetal. Foi realizada a biometria das plantas de mogno africano, de a?a?, e a quantifica??o da produ??o de palmito, onde foi poss?vel perceber que essas culturas tiveram o crescimento prejudicado devido a falta de manejo, tendo em vista que houve menos competi??o nas parcelas sem leguminosas arb?reas, onde essas culturas de interesse atingiram maiores di?metros e alturas, refletindo da mesma forma na produ??o de palmito. A aduba??o verde foi eficiente na ciclagem de nutrientes do solo, aumentando o teor da maioria dos elementos ap?s um manejo e superando vegeta??o espont?nea al?m de ter beneficiado a produ??o do feijoeiro. A inocula??o com extrato de n?dulos ? capaz de aumentar a nodula??o e o teor de nitrog?nio da parte a?rea do feijoeiro, por?m n?o aumentou a produ??o de gr?os.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-29T19:03:41Z No. of bitstreams: 1 2015 - Francine Yuriko Otsuka Rocha.pdf: 2503949 bytes, checksum: b9cea1d05fae4647a2e2459b6401ecc0 (MD5) / Made available in DSpace on 2017-06-29T19:03:41Z (GMT). No. of bitstreams: 1 2015 - Francine Yuriko Otsuka Rocha.pdf: 2503949 bytes, checksum: b9cea1d05fae4647a2e2459b6401ecc0 (MD5) Previous issue date: 2015-09-09 / CAPES / The fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is a disease of economic importance to tomato crop (Lycopersicon esculentum Mill.) because of the losses caused in the production. This study aimed to select bacteria belonging to the genus Bacillus which have antagonistic activity to fungi present in tomato producing areas. It was obtained 193 bacterial isolates from tomato roots and stems. We performed the complete sequencing 16S rRNA subunit of 32 bacteria from the work collection using the 27F and Amp2 primers. These bacteria were randomly selected, and 20 bacteria were obtained from healthy tomato plants. The results showed a high similarity to species of the genus Bacillus: B. thuringiensis, B. megaterium, B. cereus, B. toyonensis, B. stratosphericus and Paenibacillus barcinonensis. Evaluation of the functional characteristics of the bacterial strains showed that 72% possess proteolytic activity, 78% have cellulolytic activity, 48% are able to solubilize inorganic phosphate, 42% solubilize zinc oxide and 40.8% are able to produce indols. Antagonism tests carried out in vitro showed that some bacterial strains reduced the mycelial growth of the three races of the fungus when subjected to direct method (parallel streaks). An experiment was conducted using the genotypes Debora Plus, Santa Clara Miss Brazil, Perinha and Carolina in order to select a sensitive tomato genotype to the three fungal races. The Perinha variety was the most susceptible for races 1, 2 and 3 and presented the highest percentage of fungal colonization. Thus, this variety was used in a greenhouse experiments to evaluate the antagonistic role of the Bacillus strains against three fungal races. The antagonistic activity of bacteria was assessed 15 days after transplanting of the tomato seedlings to contaminated substrate. The results showed that the B. stratosphericus strain contributed to control at least 93.25% of the damage caused by race 1, strains from species B. megaterium and B. thuringiensis controlled 100% the damage caused by race 2 and at least 67.35% of the disease caused by the race 3 of the fungus. The results suggest that some strains have the potential to control race 1, 2 and 3 of the F. oxysporum f. sp. lycopersici and have functional activities that may contribute to improving the growth of tomato plants. However, field studies are needed to confirm the role of these Bacillus species during the interaction with tomato plants grown in soil contaminated with the race 1, 2 and 3 of the fusarium wilt. / A murcha-de-fus?rio causada por Fusarium oxysporum f. sp. lycopersici ? uma doen?a de grande import?ncia econ?mica para a cultura do tomateiro (Lycopersicon esculentum Mill.) devido as perdas que ocasiona na produ??o. O presente estudo teve como objetivo selecionar bact?rias pertencentes ao g?nero Bacillus que apresentam atividade antag?nica para aplica??o em ?reas produtoras de tomate infestadas pelo fungo. Foram obtidos 193 isolados de bact?rias de ra?zes e hastes de tomateiro. Foi realizado o sequenciamento completo da subunidade 16S RNAr de 32 isolados da cole??o de trabalho, utilizando os iniciadores 27F e Amp2. Essas bact?rias foram selecionadas aleatoriamente, sendo que 20 bact?rias foram obtidas de tomateiro sadio. Os resultados mostraram uma alta similaridade das estirpes com as esp?cies do g?nero Bacillus: B. thuringiensis, B. megaterium, B. cereus, B. toyonensis, B. stratosphericus, al?m da esp?cie Paenibacillus barcinonensis. Testes para avaliar as caracter?sticas funcionais das estirpes bacterianas in vitro mostraram que 72% das estirpes possuem atividade proteol?tica, 78% possuem atividade celulol?tica, 48% solubilizam fosfato inorg?nico, 42% solubilizam zinco e 40,8% s?o capazes de produzir indol. Testes de antagonismo realizados in vitro mostraram que algumas estirpes bacterianas retardaram ou reduziram o crescimento micelial das tr?s ra?as do fungo quando submetidos ao m?todo de a??o direta (estrias paralelas). Com o objetivo de selecionar um gen?tipo de tomateiro sens?vel as tr?s ra?as do fungo, foi instalado um experimento utilizando os gen?tipos D?bora Plus, Santa Clara Miss Brasil, Perinha e Carolina. A variedade Perinha foi a mais suscet?vel as ra?as 1, 2 e 3 j? que apresentou um maior percentual de coloniza??o do fungo. A atividade antag?nica das bact?rias foi avaliada 15 dias ap?s o transplantio das mudas de tomate da variedade Perinha para o substrato contaminado. Os resultados mostraram que a estirpe da esp?cie B. stratosphericus controlou cerca de 93,25% das plantas crescidas na presen?a da ra?a 1 do fungo. J? as esp?cies B. megaterium e B. thuringiensis controlaram 100% da doen?a das plantas de tomateiro cultivadas em substrato com a ra?a 2 e de 67,35% das plantas cultivadas em substrato com a ra?a 3 do fungo. Os resultados obtidos sugerem que algumas estirpes possuem potencial para controle de F. oxysporum f. sp. lycopersici ra?a 1, 2 e 3 e possuem atividades funcionais que podem contribuir para a melhoria do crescimento das plantas de tomateiro cultivadas em ?reas infestadas com o fungo. Entretanto, estudos em campo s?o necess?rios para confirmar o papel dessas esp?cies de Bacillus na intera??o com plantas de tomateiro cultivadas na presen?a das ra?as 1, 2 e 3 da murcha de fus?rio.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-08-04T15:33:18Z No. of bitstreams: 1 2009 - Fl?vio Guerra Barroso.pdf: 2034037 bytes, checksum: 1635fb4534d6b8f1c27887b03f88ac56 (MD5) / Made available in DSpace on 2017-08-04T15:33:18Z (GMT). No. of bitstreams: 1 2009 - Fl?vio Guerra Barroso.pdf: 2034037 bytes, checksum: 1635fb4534d6b8f1c27887b03f88ac56 (MD5) Previous issue date: 2009-08-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico. CNPQ / The biological contamination by invasive alien species is considered one of the greatest threats to biodiversity worldwide. However, in Brazil the information on this subject are still scarce, especially regarding the occurrence, distribution and influence of alien species on native communities, mainly in protected areas. The objective of this study was to identify exotic plant species present in the intensive use zone of Serra dos ?rg?os National Park and determine their occurrence, distribution and influence on the native plant community. The study area is located in Teres?polis and concentrates on the barragem road, which cuts across the zone of intensive use. To determine the occurrence of exotic plant species were allocated, randomly, 30 plots of 10 x 10 m along the road, located at different distances in edge-interior gradient of forest in order to identify the penetration and distribution of these species in the forest. Individuals with circumference at breast height (cbh) above 8 cm were included in the sample. To characterize plant community, phytosociological parameters were calculated as basal area, density, frequency, dominance and rates of coverage and importance. For the herbaceous layer, sub-plots of 5 x 5 m plots were installed within the existing plots, and those sampled by the method of points. We found 1,274 individuals of shrub-tree layer, distributed in 53 families, and 362 individuals of the herbaceous layer, distributed in 15 families. Only two exotic species were sampled: Impatiens walleriana Hook. f. e Hedychium coronarium J. K?nig, both herbaceous. These species were restricted to plots of class A, with penetration less than 10 meters. Its distribution is aggregated and, in the plots, they were found only in low densities, but with high frequency, suggesting behavior of weeds. The presence of these species have no influence on the tree-shrub layer but in the herbaceous layer, values of richness and abundance showed a negative correlation in the presence of these exotic species. / A contamina??o biol?gica por esp?cies ex?ticas invasoras ? considerada uma das maiores amea?as ? biodiversidade mundial. No entanto, no Brasil as informa??es sobre este assunto ainda s?o escassas, principalmente em rela??o ? ocorr?ncia, distribui??o e influ?ncia de esp?cies ex?ticas sobre as comunidades nativas, especialmente em ?reas protegidas. O objetivo deste estudo foi identificar as esp?cies vegetais ex?ticas presentes em um trecho da zona de uso intensivo do Parque Nacional da Serra dos ?rg?os e determinar sua ocorr?ncia, distribui??o e influ?ncia sobre a comunidade vegetal nativa. A ?rea de estudo localiza-se na sede Teres?polis do parque e concentra-se na estrada da barragem, que corta toda a zona de uso intensivo. Para determinar a ocorr?ncia de esp?cies vegetais ex?ticas foram alocadas 30 parcelas de 10 x 10 m ao longo da estrada, situadas a diferentes dist?ncias no gradiente borda-interior de mata a fim de identificar a penetrabilidade e distribui??o dessas esp?cies na floresta. Indiv?duos arb?reos com CAP igual ou superior a 8 cm foram inclu?dos na amostragem. Para caracterizar comunidade vegetal foram calculados par?metros fitossociol?gicos como ?rea basal, densidade, freq??ncia, domin?ncia absolutas e relativas e os ?ndices de cobertura e import?ncia. Para o estrato herb?ceo, sub-parcelas de 5 x 5 m foram alocadas dentro das parcelas j? existentes, e os indiv?duos foram amostrados atrav?s do m?todo de pontos. Foram encontrados 1.274 indiv?duos do estrado arbustivo-arb?reo, distribu?dos em 53 fam?lias, e 362 indiv?duos do estrato herb?ceo, distribu?dos em 15 fam?lias. Apenas duas esp?cies ex?ticas foram amostradas: Impatiens walleriana Hook. f. e Hedychium coronarium J. K?nig, ambas herb?ceas. Essas esp?cies estavam restritas as parcelas da classe A, com penetrabilidade inferior a 10 metros. Sua distribui??o ? agregada e, nas parcelas, elas foram encontradas somente em baixas densidades, por?m com alta freq??ncia, sugerindo um comportamento invasor. A presen?a dessas esp?cies parece n?o exercer influ?ncia sobre o estrato arbustivo-arb?reo por?m, no estrato herb?ceo, valores de riqueza e abund?ncia apresentam uma correla??o negativa quanto a presen?a dessas esp?cies ex?ticas.

Submitted by Luis Ricardo Andrade da Silva (lrasilva@uefs.br) on 2016-03-01T23:07:05Z No. of bitstreams: 1 Dissertacao_Filipe Torres.pdf: 3327655 bytes, checksum: a687ff94c9854a5e290b5d00055299e0 (MD5) / Made available in DSpace on 2016-03-01T23:07:05Z (GMT). No. of bitstreams: 1 Dissertacao_Filipe Torres.pdf: 3327655 bytes, checksum: a687ff94c9854a5e290b5d00055299e0 (MD5) Previous issue date: 2015-12-18 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / Cutaneous leishmaniasis affects 12 million people around the world. The main etiological agent in Brazil is Leishmania braziliensis. Its annotation is not very well characterized and have inaccurate regions because of the large number of hypothetical and putative genes. To solve this problem, we annotated the Leishmania braziliensis genome (MHOM / BR / 75 / M2904) with new algorithm and curated database by similarity. Initially, we downloaded these sequences of Leishmania braziliensis from NCBI and used GENSCAN and GLIMMER algorithms. We compared the predicted genes with SWISSPROT protein database. The comparison process was made by BLASTx and SWISS-PROT. We also used StructRNAFinder to predict the non-coding RNA, ncrna. We identified 25,539 ORF's, 4,916 genes, 735 ncRNA and 863 proteins. 94.75\% of predicted genes from Leishmania braziliensis were present on Leishmania panamensis genome (MHOM/PA/94/PSC-1). The comparison of ncrna number and proteins highlights a relation between chromosome and ncrna. All genes found were mapped and aligned to the Leishmania braziliensis genome and stored in a relational database model built in PHP 5.0 and MySQL. All data resulting of analyses in this work is available at www.leishdb.com. / A leishmaniose cut?nea afeta cerca 12 milh?es de pessoas ao redor do mundo. O principal agente etiol?gico dessa patologia no Brasil ? a Leishmania braziliensis. A anota??o dela n?o est? bem caracterizada e possui regi?es sem uma grande certeza devido ao grande n?mero de genes hipot?ticos e putativos. Para resolver esse problema,n?s reanotamos o genoma da Leishmania braziliensis (MHOM/BR/75/M2904) com novas vers?es de algoritmos e uma base de dados curada. Inicialmente, n?s baixamos e predizemos os genes com uma base de prote?nas. A compara??o foi feita porBLASTx e o banco de dados SWISS-PROT. Tamb?m utilizamos o preditor StructRNA Finder para predi??o de RNA?s n?o codificantes. Como resultado desse trabalho identificamos 25539 ORF?s, 4916 genes, 735 ncRNA e 863 prote?nas. Cerca de 94.75% genes preditos tamb?m estavam presentes em anota??o da Leishmania panamensis (MHOM/PA/94/PSC-1). Comparando o n?mero de rna n?o codificante e prote?nas foi evidenciado a presen?a de rela??es entre cromossomos e ncrna. Todos os genes encontrados foram mapeados no genoma da Leishmania braziliensis e armazenados em um modelo de banco de dados relacional constru?do em MySQL e PHP 5.0. Todos os dados resultantes desse trabalho est?o dispon?veis.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-06-14T22:02:36Z No. of bitstreams: 1 63-lais-cardoso-dissertacao-completa-07-08-2014.pdf: 1594778 bytes, checksum: 095a7789d1a934007d9907b518ed7989 (MD5) / Made available in DSpace on 2016-06-14T22:02:36Z (GMT). No. of bitstreams: 1 63-lais-cardoso-dissertacao-completa-07-08-2014.pdf: 1594778 bytes, checksum: 095a7789d1a934007d9907b518ed7989 (MD5) Previous issue date: 2014-08-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The species was selected to carry out the research because of the wide medicinal use for diarrhea treatment by the Brazilian northeast population. The objective of this work was to study the morphological and anatomical aspects of the leaves of Stachytarpheta jamaicensis, detect the presence of the major classes of secondary metabolites present in the crude extract of the leaves and still assessing the potential antioxidant, cytotoxic and antibacterial said extract and its fractions. To achieve the objectives, paradermic sections and cross were made to study the epidermis and its annexes. Was also analyzed characters of leaf morphology and petiole through histological sections. Phytochemical screening of crude extracts was performed by colorimetric tests and tests precipitation. From the raw methanol extract was held partitions with increasing polarity solvents (hexane, chloroform and ethyl acetate). The fractionation and purification of the partitioned extracts was performed by column chromatography (CC) and monitored by thin layer chromatography (TLC). The crude extract (EB), and fractions were subjected to test the free radical sequestration 1,1 - diphenyl-2-picrylhydrazyl (DPPH) test and the mortality of Artemia salina, to assess antioxidant activities and cytotoxic activities, respectively. To evaluate the antibacterial activity were performed the disk diffusion techniques in well and MIC (minimum inhibitory concentration) compared to the strains of Salmonella and E.coli. The blade is amphistomatic with stomata of diac?tic type, the epidermis is uniseriate, with the presence of trichomes. The results detected the presence of tannins, flavonoids, alkaloids and saponins. In the evaluation of antioxidant activity, IC 50 values (mg / ml) were found 6.62 to 446.20 for the tested extracts. For cytotoxicity, all extracts showed LC50 values (mg / ml) 211.16 to 446.20, and the hexane extract the most active (LC50 211.16). For antibacterial analysis, has not been demonstrated activity of extracts front bacteria tested. The toxicity of the leaf of this species refers caution about their safety for use by the population. / A esp?cie foi selecionada para realiza??o da pesquisa devido ? ampla utiliza??o medicinal para tratamento de diarreia pela popula??o do nordeste brasileiro. O objetivo desse trabalho foi estudar os aspectos morfoan?tomicos das folhas de Stachytarpheta jamaicensis, detectar presen?a das principais classes de metab?litos secund?rios presentes no extrato bruto de suas folhas e ainda avalia??o do potencial antioxidante, citot?xico e antibacteriano do referido extrato e suas fra??es. Para alcan?ar os objetivos, sec??es parad?rmicas e transversais foram efetuadas para estudo da epiderme e seus anexos. Analisou-se tamb?m caracteres da morfologia foliar e do pec?olo atrav?s de cortes histol?gicos. Foi realizada triagem fitoqu?mica do extrato bruto atrav?s de testes colorim?tricos e ensaios de precipita??o. A partir do extrato bruto metan?lico, realizou-se parti??es com solventes de polaridade crescente (hexano, clorof?rmio e acetato de etila). O fracionamento e purifica??o dos extratos particionados foi realizado por cromatografia em coluna (CC) e monitorado por cromatografia em camada delgada (CCD). O Extrato Bruto (EB) e as frac?es foram submetidos ao teste do sequestro do radical livre 1,1 ? difenil-2-picrilhidrazil (DPPH) e ao ensaio de letalidade da Artemia salina, para avalia??o das atividades antioxidante e citot?xica, respectivamente. Para avalia??o da atividade antibacteriana foram executadas as t?cnicas de difus?o em disco, em po?o e MIC (concentra??o inibit?ria m?nima) frente ?s cepas de Samonella e E.coli. A l?mina foliar ? anfiestom?tica, com est?matos do tipo diac?tico, a epiderme ? uniestratificada, com presen?a de tricomas tectores. Os resultados detectaram presen?a de taninos, flavonoides, alcaloides e saponinas. Na avalia??o da atividade antioxidante, os valores de IC50 (mg/ml) encontrados foram de 6,62 a 446,20 para os extratos testados. Quanto ? citotoxicidade, todos os extratos apresentaram valores de LC50 (mg/ml) de 211,16 a 446,20, sendo o extrato hex?nico o mais ativo (LC50 211,16) . Quanto ? an?lise antibacteriana, n?o foi demonstrada atividade dos extratos frente as bact?rias testadas. A toxicidade apresentada pela folha dessa esp?cie remete cautela quanto ? sua seguran?a para uso pela popula??o.

Made available in DSpace on 2016-04-28T14:56:17Z (GMT). No. of bitstreams: 1 2009 - Priscila Nogueira Matos.pdf: 1723305 bytes, checksum: 89e42c980c370e9eec2db3e9b08306ae (MD5) Previous issue date: 2009-03-31 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The Mining is considered the activity can cause a great impact in the environment, involving the vegetation and subsoil layers remove. After the finish of mineral sources, mandatorily these areas require recovery measures. This study aimed to evaluate the initial establishment of three genotypes of palm oil: C2501, C2301 and C2528, indicated by Embrapa Amaz?nia ocidental, in area of mining in the northwest Par?. Was also evaluated the soil fauna, the occurrence of nitrogen fixing bacteria and arbuscular mycorrhizal fungi associated with culture. The establishment of culture was evaluated for survival, height, number of leaves and nutritional status of plants and soil fertility. The impact on soil fauna was evaluated with samples in the experimental area and in areas of reforestation and forest adjacent to it. To determine the presence of mycorrhizal fungi and diazotrophic bacteria samples were taken from roots and soil in the palm oil. The amount of the plant N derived from BNF was determined with the natural abundance of 15N technique. The mortality of the plants was low, but growth was not strong, this may be related to low P in soil and periods of drought. In this initial stage of establishing the symbiosis with diazotrophic bacteria was inefficient where only the genotypes C2501 and C2528 were 4,5 and 4,8% respectively of N derived from BNF, with great variability in the population of bacteria. The mycorrhizal fungi no showed differences in sampled areas and this result may be related to water deficit and sampling period in the dry season. The composition of soil fauna not differ in the areas of palm oil plantation with and without green manure, and conventional reforestation, however not present the same diversity of the forest. / A minera??o ? considerada uma atividade econ?mica que causa um grande impacto ao ambiente, envolvendo a retirada da vegeta??o e camadas do subsolo. Ap?s a exaust?o dos recursos minerais, estas ?reas necessitam obrigatoriamente de medidas de recupera??o. O objetivo deste trabalho foi avaliar o estabelecimento inicial de tr?s gen?tipos de dend?: C2501, C2301 e C2528, indicados pela Embrapa Amaz?nia Ocidental, em ?reas de est?ril da Minera??o de bauxita no noroeste do Par?. Foram avaliados tamb?m a fauna do solo, a ocorr?ncia de bact?rias fixadoras de nitrog?nio e fungos micorr?zicos arbusculares associados ? cultura. O estabelecimento da cultura foi avaliado pela sobreviv?ncia, altura, n?mero de folhas e estado nutricional das plantas, e a fertilidade do solo. O impacto na fauna do solo foi avaliado na ?rea experimental e em ?reas de floresta prim?ria e reflorestamento adjacentes a este. A presen?a de bact?rias diazotr?ficas e fungos micorr?zicos foram determinadas em amostras de ra?zes e solo na ?rea do dend?. A quantidade de N na planta derivado da FBN foi determinada pela t?cnica de abund?ncia natural de 15N. A mortalidade das plantas foi considerada baixa apesar de seu crescimento n?o ter sido vigoroso podendo este estar relacionado ao baixo teor de P no solo e aos per?odos de stress h?drico por falta de ?gua. Neste est?gio inicial de implanta??o a simbiose com bact?rias diazotr?ficas foi pouco eficiente onde apenas os gen?tipos C2501 e C2528 tiveram 4,5 e 4,8% respectivamente de N derivado da FBN, com grande variabilidade na popula??o de bact?rias. Os fungos micorr?zicos n?o apresentaram diferen?as nas ?reas analisadas, tal resultado pode estar relacionado com a defici?ncia h?drica e o per?odo de coleta na ?poca seca. A composi??o da fauna do solo nas ?reas de plantio de dend? com e sem aduba??o n?o diferiram entre si e em rela??o ao reflorestamento convencional, n?o apresentando, entretanto a mesma diversidade da floresta prim?ria.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-03T11:52:14Z No. of bitstreams: 1 2011 - Gabriela Bueno B. Silva.pdf: 16499028 bytes, checksum: f77bf195f420f4227c29b60a75895d0d (MD5) / Made available in DSpace on 2016-08-03T11:52:14Z (GMT). No. of bitstreams: 1 2011 - Gabriela Bueno B. Silva.pdf: 16499028 bytes, checksum: f77bf195f420f4227c29b60a75895d0d (MD5) Previous issue date: 2011-04-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / BITTENCOURT-SILVA, Gabriela Bueno. Insular Anuran (Amphibia) Communities of the Southeast Coast of Brazil: Taxonomic Composition and Relationship to the History of the Islands. 2011. 72p. Dissertation (Master of Science in Animal Biology). Instituto de Biologia, Departamento de Biologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2011. A data set containing a list of the known species of frogs that occur near shore and on eight islands of the south coast of the State of Rio de Janeiro and north of the State of S?o Paulo was produced. For that, I compiled data from the literature and conducted inventories of a few of the islands. This list included 67 anuran species belonging to 11 families for eight islands and 117 species and 14 families for two localities on the mainland. The pattern of richness between islands was analyzed in respect to the island area and the composition of habitats used by the anurans for reproduction. Insular structural complexity was also evaluated in this regard. The results of correlation analyses of area vs. species richness and species richness vs. number of reproductive habitats were highly significant indicating that species richness prediction in fragmented environments depends on both factors. Nestedness analyses using the metric NODF was performed in an attempt to investigate whether shared species absences among the islands are the result of a random process or represents a pattern indicative of ordered loss of species. The predicted pattern was confirmed ? that is, that species loss is nonrandom and that this was possibly caused by habitat loss. The role played by climatic events at the beginning of the Holocene and of the availability of reproductive habitats in the islands upon species loss is discussed. Furthermore, it is discussed how these results can help to guide conservation strategies of anuran species. / BITTENCOURT-SILVA, Gabriela Bueno. Comunidades de Anf?bios Anuros Insulares do Litoral Sudeste do Brasil: Composi??o Taxon?mica e Rela??es com a Hist?ria de Forma??o das Ilhas. 2011. 72p. Disserta??o (Mestrado em Biologia Animal). Instituto de Biologia, Departamento de Biologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2011. Atrav?s de invent?rios e compila??o de dados da literatura levantou-se a composi??o de esp?cies de anf?bios anuros de oito ilhas, sendo seis da Costa Verde do Estado do Rio de Janeiro e duas do litoral norte do Estado de S?o Paulo. Al?m das ilhas, foram tamb?m compilados da literatura os invent?rios de duas comunidades representativas do litoral dos dois Estados. Adicionalmente, foram registradas 67 esp?cies, pertencentes a 11 fam?lias de anuros nas ilhas e 117 esp?cies e 14 fam?lias para o continente. O padr?o de distribui??o de riqueza das ilhas foi avaliado em rela??o ? ?rea das ilhas, ? composi??o de ambientes usados pelos anuros para reprodu??o e ? complexidade estrutural das ilhas. As an?lises de correla??o da riqueza de esp?cies vs. ?rea e riqueza de esp?cies vs. n?mero de ambientes reprodutivos apresentam resultados significativos, que s?o indicativos que a riqueza de esp?cies em ambientes fragmentados depende da intera??o de ambos os fatores. A fim de avaliar se as aus?ncias compartilhadas de esp?cies entre as ilhas ocorrem ao acaso ou encontram-se estruturadas, realizou-se uma an?lise de aninhamento com o aux?lio da m?trica NODF. O padr?o previsto foi confirmado e a perda de diversidade ? discutida em rela??o ? eventos clim?ticos do in?cio do Holoceno e ? disponibilidade de ambientes reprodutivos nas ilhas. Discute-se ainda como os resultados desse estudo podem servir para orientar programas de conserva??o de esp?cies de anf?bios anuros

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-15T12:14:46Z No. of bitstreams: 1 2011 - Daiane Ouvernay Fernandes da Silva.pdf: 7586481 bytes, checksum: 5a97593f1df40687d3039aaf482f7a65 (MD5) / Made available in DSpace on 2016-09-15T12:14:46Z (GMT). No. of bitstreams: 1 2011 - Daiane Ouvernay Fernandes da Silva.pdf: 7586481 bytes, checksum: 5a97593f1df40687d3039aaf482f7a65 (MD5) Previous issue date: 2011-06-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This study investigated the relationship between species of frogs, lizards, gradients environmental and insularity. We could detect a strong relationship between lizards in open habitats and anthropic as well as a strong influence of the area to the richness and abundance of lizards, rather than habitat heterogeneity. The community was the most diverse State Park Cunhambebe. The lizards of island environments of the Sepetiba Bay showed a strong relationship between abundance and area, supporting the hypothesis of Ecological Release. The species Cycloramphus sp., Thoropa miliaris, Hylodes asper, H. phyllodes, Phasmahyla cruzi and Scinax trapicheiroi are those with lower values of tolerance of distance from this physiognomy and sometimes only occur in this environment. / Neste estudo foram investigadas as rela??es entre esp?cies de anf?bios anuros, lagartos, gradientes ambientais e insularidade. Nota-se uma forte rela??o dos lagartos com ambientes abertos e antr?picos, bem como uma forte influ?ncia da ?rea para a riqueza e abund?ncia dos lagartos, mais que a heterogeneidade de habitats. A comunidade mais diversa foi a do Parque Estadual do Cunhambebe. Os lagartos de ambientes insulares da Ba?a de Sepetiba demonstraram forte rela??o entre abund?ncia e ?rea, corroborando com a hip?tese de Libera??o ecol?gica. As esp?cies Cycloramphus sp., Thoropa miliaris, Hylodes asper, H. phyllodes, Phasmahyla cruzi e Scinax trapicheiroi s?o as que possuem menores valores de toler?ncia de dist?ncia da cachoeira e, algumas vezes, s? ocorrem neste ambiente