Aquatic invasive species are a major threat to native freshwater biodiversity. The North American signal crayfish Pacifastacus leniusculus was introduced to Great Britain during the 1970s and is now widely distributed throughout England, Wales and Scotland. First recorded in Scotland in 1995, P. leniusculus is now established at more than twenty sites. The only other introduced crayfish species present in Scotland is the white-clawed crayfish Austropotamobius pallipes. A. pallipes is restricted to only two locations in Scotland, Loch Croispol and Whitemoss Reservoir. P. leniusculus negatively impacts macrophytes, invertebrates and fish though ecological and physical processes. Additionally, P. leniusculus has displaced A. pallipes throughout much of its native range within Great Britain due to competition and disease. Consequently, the two A. pallipes populations in Scotland have a high conservation value. This PhD study aimed to improve understanding of P. leniusculus invasion success by examining trophic dynamics and to develop methodologies that could improve the detection and control of P. leniusculus populations in Scotland. Stable isotope analysis was used to determine the diet composition, trophic position and whether an ontogenetic dietary shift occurs in the Loch Ken population of P. leniusculus. Bayesian mixing models indicated that P. leniusculus in Loch Ken do exhibit an ontogenetic dietary shift. Additionally, individuals of all sizes occupied the trophic position of a predator in Loch Ken suggesting that invertebrates and fish constitute an important component of P. leniusculus diet. Stable isotope analysis was used once again to compare the isotopic niche width and diet composition of P. leniusculus populations from Loch Ken and A. pallipes populations from Loch Croispol and Whitemoss Reservoir. At the species level, A. pallipes exhibited a larger niche width than that of P. leniusculus. At the population level, the isotopic signatures of the A. pallipes populations were considerably different from each other suggesting an overestimation of A. pallipes’ niche width at species level. Results showed no dietary overlap between species and Bayesian mixing models suggested P. leniusculus and A. pallipes were consuming different resources, indicating there would be no direct competition for food resources if they were to co-occur. A plus-maze study was used to determine if P. leniusculus exhibited a preference for one of four food attractants (Oncorhynchus mykiss, P. leniusculus, beef or vegetation), which could be used to improve trapping efficiency. In the maze system, P. leniusculus exhibited no preference for any food attractant presented. This would suggest that either the maze was not a good model or food attractants would not improve trapping efficiency of P. leniusculus. Additionally, a comparative investigation into the use of gill nets as a method to control P. leniusculus was conducted. Results showed that the net type and the presence of fish entangled in the net influenced the number of P. leniusculus caught. Finally, environmental DNA (eDNA) was used and evaluated for detection of P. leniusculus. A robust quantitative Polymerase Chain Reaction (qPCR) assay and DNA extraction protocol were developed. Using the developed qPCR assay, P. leniusculus eDNA was detected in controlled aquaria conditions but not in environmental water samples collected from the field. Furthermore, the quantities of P. leniusculus eDNA declined in aquaria conditions while individuals were still present suggesting the mechanisms for eDNA release by P. leniusculus are complex. Stable isotope analysis indicates that P. leniusculus exhibit an ontogenetic dietary shift, and in each life stage, P. leniusculus function as an omnivore but occupy the trophic position of a predator. Niche width analysis revealed that the diet of P. leniusculus was less general than that observed in A. pallipes and thus diet of P. leniusculus may not be responsible for invasive success. Food attractants will not enhance trapping efficiency but nets may present a potential new method to control P. leniusculus. Similarly, eDNA presents a promising new method for rapid detection of P. leniusculus. It will not be possible to eradicate P. leniusculus in Scotland but the findings of this PhD may help prevent establishment of new populations. These results should be incorporated into future management strategies for P. leniusculus populations in Scotland and may have broader applications in Great Britain and Europe.

Le chat haret est l’un des prédateurs invasifs les plus dommageables pour la biodiversité insulaire. Sa présence est associée à une perte de biodiversité sur l’ensemble des îles sur lesquelles il est établi, et où il constitue une menace pour de nombreuses espèces de vertébrés souvent endémiques et menacés. En Nouvelle-Calédonie des populations de chats harets sont présentes dans tous les milieux et habitats et l’étude de son écologie et de ses impacts sur la faune ont fait l’objet de ce travail de thèse. L’analyse du régime alimentaire sur 14 sites d’études représentatifs des 4 habitats majeurs a révélé un régime très diversifié et une forte prédation sur les vertébrés natifs et notamment sur le groupe des scinques, des roussettes et des pétrels. Parmi les 44 espèces de vertébrés retrouvées dans le régime alimentaire de ce prédateur invasif, la plupart sont endémiques et 20 listées comme menacées sur la liste rouge mondiale de l’UICN. Le suivi des déplacements d’individus équipés de colliers GPS au niveau d’une presqu’île abritant une importante colonie d’oiseaux marins, a permis de mettre en évidence de grands domaines vitaux pour les mâles, des domaines vitaux petits pour les femelles et des patrons de déplacements liés aux différentes étapes du cycle reproducteur des oiseaux marins. Ces éléments, couplés aux analyses de régime alimentaire suggèrent une prédation à une large échelle géographique, à la fois sur les adultes reproducteurs mais également sur les jeunes oiseaux proches de l’envol. Une opération expérimentale de contrôle d’une population de chats harets a été conduite sur cette presqu’île et a montré une faible durabilité des effets de la suppression des individus sur les densités observées et une rapide recolonisation du site. Les résultats de ce travail plaident pour la mise en place de mesures de limitation des impacts occasionnés et permettent en particulier de cibler les habitats de maquis et forêt humide comme prioritaires en matière de limitation des abondances. / Feral cat (Felis catus) is one of the most successful and harmful invasive predator species for island biodiversity. The presence of this alien predator species generally lead to dramatic loss of native island biodiversity and represents a serious threat for numerous endemic and threatened species. Feral cats have invaded the whole New-Caledonian archipelago and all its habitats. In this study, we focused on the ecology and impacts of this invasive predator on the outstanding endemic fauna found in the different habitats of the exceptional New-Caledonia biodiversity hotspot. Feral cat diet analyses on 14 selected sites representing the 4 main natural habitats revealed a high diversified diet and high predation rates on native species particularly on squamates, flying foxes and petrels. Among the 44 vertebrates species found into the feral cat diet, 20 are IUCN red-listed threatened species. Cat movements of eleven feral cats fitted with GPS collars have been studied in a western coast Peninsula hosting an important seabird colony. Male cats showed large home ranges while female showed small home ranges. Feral cats exhibited important movements within the studied peninsula linked with the breeding cycles of seabirds. GPS data coupled with dietary informations suggested a predation that concerned both breeding adults and fledgings bird, and at a large geographic scale as some feral cats have their core home range distant to the colony (>3km). We evaluated the effects of a high level but intense cat control on this site that showed a low sustainability of feral cat culling and a rapid recolonization process. Our results are pleading for the future limitation of feral cat impacts and call to focus first abundance limitation measures on maquis mosaic and humid forest habitats This study also provided information on the spatial extent and intensity of future control measures in the special context of a large and highly invaded island.

Abstract not available

Invasive species represent a major concern; they can result in serious ecological and economic losses and are recognised as one of the most serious threats to global species diversity. Plant invasions are of particular concern in New Zealand, which has high proportions of both naturalised and endemic plant species. In this thesis I focussed on the invasive plant Hieracium lepidulum, an exotic weed introduced from Europe to New Zealand prior to 1941. It is invasive in a variety of habitats in the South Island, where it has steadily increased in distribution and abundance over the last 50 years, and is thought to have detrimental impacts on native plant communities. I investigated factors influencing its invasion success and tested for impacts on native plant communities, making extensive use of existing plots into which H. lepidulum was experimentally introduced in 2003. I examined how community richness, turnover, resource availability and propagule pressure of the invader interacted to determine the invasion success of H. lepidulum. Results differed markedly above and below treeline. Above treeline, plots with higher richness and turnover were more invaded; below treeline, plots with higher available light were more invaded. In both habitats, these findings were modified by the influence of propagule pressure; at low propagule pressure, site characteristics were non-significant in explaining invasion success, while at higher propagule pressure these effects became significant. To test for impacts resulting in altered community composition and structure, I looked for changes in community richness, diversity and evenness subsequent to H. lepidulum introduction. As impacts may be more apparent at fine spatial scales, I made measurements at a 5 x 5 cm cell scale in addition to the established 30 x 30 cm plot scale. Plot species richness increased from 2003 to 2009 and a component of this increase was associated with H. lepidulum density. Other relationships between the plant community and H. lepidulum were generally non-significant. Results showed that H. lepidulum has had no negative effects on community richness, evenness or diversity. Despite being able to opportunistically colonise grassland sites with high turnover, and forest sites subject to canopy disturbance, dependant on propagule pressure, it appears H. lepidulum has not impacted community composition or structure.

Invasive exotic species threaten the biodiversity and function of native ecosystems. Existing models, attempting to predict and control successful invaders, often emphasize isolated stages of in their life history and fail to formalize interactions between exotic species and recipient environments. In order to elucidate key mechanisms in the success of select invaders, I investigated the role of dispersal, establishment, proliferation, and persistence in their threat to natural areas. Focusing on Riding Mountain National Park, Manitoba, Canada, I integrated the native climatic range and biological traits of 251 exotic vascular plants reported inside and outside the park. Based on their climatic range in Europe, 155 among 174 exotic plant species absent from the Park were predicted to establish within its boundaries; among these, 40 clonal perennials were considered the highest threat to the Park’s biodiversity. Focusing on smooth brome (Bromus inermis Leyss.), a Eurasian perennial, threatening the structure and function of native prairies throughout the Great Plains, I extended my research to investigate the role of dispersal, establishment, proliferation, and persistence in characterizing its threat to the endemic diversity of northern fescue prairies, protected within Riding Mountain National Park. Patterns of smooth brome invasions were contingent on the type of propagules dispersed. The shallow dispersal gradient of individual florets combined with the steeper gradient of panicles and spikelets suggested that smooth brome is capable of simultaneously invading along dense fronts as well as by establishing isolated foci. While low correlations between the number of dispersed seeds and their recruitment suggested post-dispersal transport, seedling establishment remained contingent on prairie diversity. Seedling biomass increased with declining plant diversity, however, its impact depended on the availability of soil nitrogen. As a result, disturbed areas, preserving the root function of native plants, resisted smooth brome establishment. Even though low nitrogen contributed to a decline in seedling biomass, physiological integration between ramets facilitated their vegetative proliferation in low resource environments. Despite its rapid establishment and proliferation, smooth brome productivity declined at the center of invading clones. Although field and greenhouse observations failed to implicate soilborne pathogens, reasons for the observed decline remain unresolved. My research demonstrates that while Riding Mountain National Park and other natural areas in western Canada will continue to be impacted by exotic plants, integrating key stages in their life history provides an important conceptual framework in predicting their threat to natural areas and prioritizing management. / October 2008

Brook trout invasion into bull trout streams is variable, and likely influenced by a suite of biotic and abiotic factors. Field observations revealed that brook trout dominated the fish community over bull trout in warmer sites that had undercut banks; in contrast, bull trout dominated in colder sites that had a high amount of large substrate cover, and where alternate non-native species were present. Laboratory studies of competition between the two species revealed that bull trout use a scramble foraging tactic, whereas brook trout use a territorial tactic. Bull trout outcompeted brook trout when fish density was low and habitat complexity was high, as this scenario reduced the effectiveness of the aggressive territorial foraging strategy of brook trout. Bull trout from a migratory population competed more successfully against brook trout and had higher rates of oxygen consumption than those from a resident population. This combined field-lab study points to some of the abiotic and biotic factors that affect competition between the two species, and may influence the outcome of brook trout invasion into bull trout streams. / xiv, 184 leaves : ill. ; 29 cm

Invasive exotic species threaten the biodiversity and function of native ecosystems. Existing models, attempting to predict and control successful invaders, often emphasize isolated stages of in their life history and fail to formalize interactions between exotic species and recipient environments. In order to elucidate key mechanisms in the success of select invaders, I investigated the role of dispersal, establishment, proliferation, and persistence in their threat to natural areas. Focusing on Riding Mountain National Park, Manitoba, Canada, I integrated the native climatic range and biological traits of 251 exotic vascular plants reported inside and outside the park. Based on their climatic range in Europe, 155 among 174 exotic plant species absent from the Park were predicted to establish within its boundaries; among these, 40 clonal perennials were considered the highest threat to the Park’s biodiversity. Focusing on smooth brome (Bromus inermis Leyss.), a Eurasian perennial, threatening the structure and function of native prairies throughout the Great Plains, I extended my research to investigate the role of dispersal, establishment, proliferation, and persistence in characterizing its threat to the endemic diversity of northern fescue prairies, protected within Riding Mountain National Park. Patterns of smooth brome invasions were contingent on the type of propagules dispersed. The shallow dispersal gradient of individual florets combined with the steeper gradient of panicles and spikelets suggested that smooth brome is capable of simultaneously invading along dense fronts as well as by establishing isolated foci. While low correlations between the number of dispersed seeds and their recruitment suggested post-dispersal transport, seedling establishment remained contingent on prairie diversity. Seedling biomass increased with declining plant diversity, however, its impact depended on the availability of soil nitrogen. As a result, disturbed areas, preserving the root function of native plants, resisted smooth brome establishment. Even though low nitrogen contributed to a decline in seedling biomass, physiological integration between ramets facilitated their vegetative proliferation in low resource environments. Despite its rapid establishment and proliferation, smooth brome productivity declined at the center of invading clones. Although field and greenhouse observations failed to implicate soilborne pathogens, reasons for the observed decline remain unresolved. My research demonstrates that while Riding Mountain National Park and other natural areas in western Canada will continue to be impacted by exotic plants, integrating key stages in their life history provides an important conceptual framework in predicting their threat to natural areas and prioritizing management.

Invasive exotic species threaten the biodiversity and function of native ecosystems. Existing models, attempting to predict and control successful invaders, often emphasize isolated stages of in their life history and fail to formalize interactions between exotic species and recipient environments. In order to elucidate key mechanisms in the success of select invaders, I investigated the role of dispersal, establishment, proliferation, and persistence in their threat to natural areas. Focusing on Riding Mountain National Park, Manitoba, Canada, I integrated the native climatic range and biological traits of 251 exotic vascular plants reported inside and outside the park. Based on their climatic range in Europe, 155 among 174 exotic plant species absent from the Park were predicted to establish within its boundaries; among these, 40 clonal perennials were considered the highest threat to the Park’s biodiversity. Focusing on smooth brome (Bromus inermis Leyss.), a Eurasian perennial, threatening the structure and function of native prairies throughout the Great Plains, I extended my research to investigate the role of dispersal, establishment, proliferation, and persistence in characterizing its threat to the endemic diversity of northern fescue prairies, protected within Riding Mountain National Park. Patterns of smooth brome invasions were contingent on the type of propagules dispersed. The shallow dispersal gradient of individual florets combined with the steeper gradient of panicles and spikelets suggested that smooth brome is capable of simultaneously invading along dense fronts as well as by establishing isolated foci. While low correlations between the number of dispersed seeds and their recruitment suggested post-dispersal transport, seedling establishment remained contingent on prairie diversity. Seedling biomass increased with declining plant diversity, however, its impact depended on the availability of soil nitrogen. As a result, disturbed areas, preserving the root function of native plants, resisted smooth brome establishment. Even though low nitrogen contributed to a decline in seedling biomass, physiological integration between ramets facilitated their vegetative proliferation in low resource environments. Despite its rapid establishment and proliferation, smooth brome productivity declined at the center of invading clones. Although field and greenhouse observations failed to implicate soilborne pathogens, reasons for the observed decline remain unresolved. My research demonstrates that while Riding Mountain National Park and other natural areas in western Canada will continue to be impacted by exotic plants, integrating key stages in their life history provides an important conceptual framework in predicting their threat to natural areas and prioritizing management.

La present tesi intenta obtenir noves dades referents a la biologia i ecologia de la formiga argentina que poden arribar a ser cabdals per al disseny o millora de noves metodologies de control de l'expansió de la plaga en ecosistemes naturals. Així doncs, l'estudi de l'activitat de cerca d'aliment i del seu espectre dietari en ecosistemes naturals, juntament amb el de les fluctuacions estacionals de la densitat de reines en nius naturals, obre les portes a la millora de mètodes de control de la plaga mitjançant esquers tòxics d'efecte retardat i a l'aplicació de noves metodologies de control sense l'ús d'agents químics basats en l'eliminació massiva de reines. La tesi també aporta noves dades sobre la fisiologia reproductiva de l'espècie en relació a la temperatura, la qual cosa permet la integració de l'aspecte fisiològic en models de predicció del rang potencial d'establiment de la plaga en ecosistemes naturals, aspecte fins ara pràcticament oblidat en aquest tipus de models predictius. / The present work attempts to obtain new information about the biology and ecology of this species in order to improve or design new control methods to stop the expansion of the plague in natural ecosystems. In that sense, the study of its foraging activity and dietary spectrum in natural environments, as well as the report of its annual fluctuations in the queen's densities of natural nests, open the door to the improvement of control methodologies based on the use of toxic baits, as well as to the design of new control methods based on the mechanical elimination of queens without the use of chemical procedures. This work also provides new data about the species' reproductive physiology according to temperature, which allow the application in predictive models of the physiological needs of the species to establish in a certain natural area.

L’objectif principal de cette thèse est d’identifier les facteurs urbains et les caractéristiques biologiques de Reticulitermes flavipes qui ont permis à cette espèce invasive de coloniser et de persister en milieu urbain. Plus précisément, les études développées ont visé à mieux comprendre l’origine des colonies introduites à Paris, de déterminer comment celles-ci étaient arrivées en ville et, comment, malgré les moyens de lutte mis en oeuvre au cours de ces dernières décennies elles ont persisté dans le paysage parisien. Une première étude nous a permis de voir que les colonies présentes à Paris sont capables de persister et de réinfester des zones qui ont été précédemment traitées. Ces réinfestations sont possibles grâce aux modes de reproduction (néoténie) et de dispersion (par bouturage) de cette espèce qui lui permettent de survivre localement et de recoloniser des zones où les colonies avaient été partiellement éradiquées et ce, même après 15 ans. Dans une seconde étude, nous avons pu montrer deux facteurs majeurs pouvant expliquer la distribution et la propagation de R. flavipes à Paris : la structure complexe des colonies observées et la combinaison spécifique des variables de l’environnement parisien. Enfin, dans la troisième étude, nous avons décrit la dynamique d’expansion de cette espèce à des échelles nationales et régionales. Cette étude révèle des patterns de distribution variés, reflétant les caractéristiques propres des populations invasives de cette espèce en France. Dans ce travail, nous avons discuté de l’implication des caractéristiques biologiques et paysagères sur le succès invasif de cette espèce. Au vue des données obtenues au cours de cette thèse, nous avons également précisé les scénarios concernant l’histoire de l’invasion de ce termite en France, puis nous avons présenté quelques outils et préconisations qui pourraient permettre, selon nous, d’améliorer la lutte contre cet insecte nuisible en ville. / The main objective of this thesis is to identify the urban factors and biological characteristics of Reticulitermes flavipes which have allowed this invasive species to colonize and persist in urban habitat. More specifically, the developed studies aimed at better understanding the origin of introduced colonies in Paris as well as determining how they persisted in the Parisian landscape, despite implementing pest control during the last decades. A first study allowed us to observe that Parisian colonies were able to persist and reinfest areas which were previously treated. These reinfestations are possible thanks to the mode of reproduction (neoteny) and the way of dispersal (by budding) of this species, which allows it to locally survive and recolonize areas where colonies had been partially eradicated, even fifteen years later. In a second study, we were able to highlight two main factors which could explain the distribution and propagation of R. flavipes in Paris: the observed complex colony structure and the specific combination of the Parisian environmental variables. Finally, in a third study, we were able to identify its dynamic of expansion at national and regional scales which showed varied distribution patterns, reflecting the peculiar characteristics of these invasive species populations in France. In this piece of work, we analyzed the implications of the biological and landscape characteristics on the successful invasion of this species. In the view of the data obtained in this thesis, we also suggested some scenarios on the invasion history of this termite species in France and we provided tools and recommendations which, according to us, could allow the improvement of pest management of this insect in cities.