Relevance of the problem. Forest compartment under the conditions of intensive forestry is the main unit of management and inventory (Antanaitis, 1961; Franklin, 2001). Regular, every 10 years, standwise forest inventory requires great expenditures on compartment delineation and their depicting in cartographic material. Different executives, guided by the same standards, but without objective forest compartment delineation methodics, may divide one and the same object into compartments in different ways (Rutkauskas, Kenstavičius, 1978). Therefore, the variability of forest compartment boundaries is artificially increased. Under the absence of clear boundaries between compartments in the forest and considering that the whole forest is homogeneous, compartment characteristics are usually ascertained ignoring their peculiarities at the edges. This comprises preconditions for the appearance of systemic forest inventory deviations (Антанайтис и др., 1975; Kuliešis, Kasperavičius, 2000; Brassel, Lichke, 2001). The accuracy of cartographic material and the influence of deviations on estimation reliability of forest resources are not evaluated (Gemmell et al., 1991; Fish, 2000; Franklin, 2001). Compartment edges, occupying in the forests of the country almost one third of the whole area of stands, are important not only from inventory, but also from forest stand growing viewpoint. What are wood increment losses, or what additional increment may be obtained due to differing growth... [to full text]

ABCD2 (D2) has been proposed as a peroxisomal long-chain acyl-CoA transporter that is essential for very long chain fatty acid metabolism. In the livers of mice, D2 is highly induced by fenofibrate, a PPARα ligand that has been widely used as a lipid lowering agent in the treatment of hypertriglyceridemia. To determine if D2 is a modifier of fibrate responses, wild-type and D2 deficient mice were treated with fenofibrate for 14 days. The absence of D2 altered expression of gene clusters associated with lipid metabolism, including PPARα signaling. Using 3T3-L1 adipocytes, which express high levels of D2, we confirmed that knock-down of D2 modified genomic responses to fibrate treatment. We next evaluated the impact of D2 on effects of fibrates in a mouse model of dietinduced obesity. Fenofibrate treatment opposed the development of obesity, hypertriglyceridemia, and insulin resistance. However, these effects were unaffected by D2 genotype. We concluded that D2 can modulate genomic responses to fibrates, but that these effects are not sufficiently robust to alter the effects of fibrates on diet-induced obesity phenotypes. Although proposed as a peroxisomal transporter, the intracellular localization of D2, especially in adipose tissue, has not been validated with direct experimental evidence. Sequential centrifugation of mouse adipose homogenates generated a fraction enriched with D2, but lacked well-known peroxisome markers including catalase, PEX19, and ABCD3 (D3). Electron microscopic imaging of this fraction confirmed the presence of D2 protein on an organelle with evidence of a dense matrix and a diameter of ~200 nm, the typical structure and size of a microperoxisome. D2 and PEX19 antibodies recognized distinct structures in mouse adipose. Immunoisolation of the D2-containing compartment from adipose tissue confirmed the scarcity of PEX19. Proteomic profiling of the D2 compartment revealed the presence of proteins associated peroxisome, endoplasmic reticulum (ER), and mitochondria. We conclude that D2 is localized to a distinct subclass of peroxisomes that lack many peroxisome proteins and may physically associate with mitochondria and the ER.

The interplay between biochemical signals and mechanical processes during animal development is key for the formation of tissues and organs with distinct shapes and functions. An important step during the formation of many tissues is the formation of compartment boundaries which separate cells of different fates and functions. Compartment boundaries are lineage restrictions that are characterized by a straight morphology. Biochemical signaling across compartment boundaries induce the expression of morphogens in the cells along the boundaries. These morphogens then act at long-range to direct growth and patterning of the whole tissue. Compartment boundaries stabilize the position of morphogens and thereby contribute to proper tissue development. The straight morphology of compartment boundaries is challenged by cell rearrangements caused by cell division and tissue reshaping. Physical mechanisms are therefore required to maintain the straight morphology of compartment boundaries. The anteroposterior (A/P) compartment boundary in the developing Drosophila melanogaster wing is established by biochemical signals. Furthermore, mechanical processes are required to maintain the straight shape of the A/P boundary. Recent studies show that mechanical tension mediated by actomyosin motor proteins is increased along the A/P boundary. However, it was not understood how biochemical signals interact with mechanical processes to maintain the A/P boundary. Here I provide the first evidence that Hedgehog signaling regulates mechanical tension along the A/P boundary. I was able to show that differences in Hedgehog (Hh) signal transduction activity between the anterior and posterior compartments are necessary and sufficient to maintain the straight shape of the A/P boundary, which is crucial for patterning and growth of the adult wing. Moreover, differences in Hh signal transduction activity are necessary and sufficient for the increase in mechanical tension along the A/P boundary. In addition, differences in Hh signal transduction activity are sufficient to generate smooth borders and to increase mechanical tension along ectopic interfaces. Furthermore, the differential expression of the transmembrane protein Capricious is sufficient to increase mechanical tension along ectopic interfaces. It was previously suggested that mechanical tension is generated by an actomyosin-cable through which the increase in mechanical tension is transmitted between the junctions along the A/P boundary. Here I show that mechanical tension is generated locally at each cell bond and not transmitted between junctions by an actomyosin cable. My results provide new insights for our understanding of the interplay between biochemical signals and mechanical processes during animal development.

Transporting cattle from southern Alberta into the United States (US) plays a substantial economic role in the western Canadian beef industry. Thermal environments within cattle transport trailers are dependent on ambient conditions, and if inadequately managed, can be a welfare concern. To effectively manage cattle transport, the environmental conditions throughout the livestock trailer must be understood. The objective of the present study was to investigate the trailer micro-climate and welfare during 5-paired commercial long-haul transports of slaughter cattle from Alberta, Canada to Washington State, US during summer months. In addition, the effect of compartment location and trailer porosity (8.7% vs 9.6%) on trailer micro-climate, shrink and core body temperature were also investigated during the warmest in-transit hour and stationary events. The compartment location had an effect on micro-climate variables where the upper compartment had greater (P < 0.05) temperature than the bottom deck compartments and relative humidity variables had the opposite effect for both the warmest in-transit hour and stationary events. There was also an effect of trailer porosity on micro-climate variables where it was generally warmer in the trailer with the higher porosity in the stationary event. Differences between trailers included 2 additional roof hatches on the trailer with lower side-wall porosity and lower internal temperatures, which could suggest the location of the trailer porosity, could be important for heat and moisture exchange during transit. The nose of the trailer with higher porosity had generally warmer internal conditions (larger T(trailer)°C and THI(trailer)) than the trailer with lower porosity. This study also found that the temperatures inside the trailer can be 10.5°C greater than ambient temperatures during stationary events and 9°C greater than ambient levels during the warmest in-transit hour. The average amount of per-animal weight loss was 4.3 0.3 % and was affected by trailer porosity and compartment, which followed the trends in thermal environment variables. The transit status (stationary or in-transit) and trailer porosity affected the vaginal core body temperature of the heifers in transit. The core body temperature was greater during stationary events for animals transported in the trailer with lower porosity. It is suggested that the lower side-wall porosity and/or the shape of perforation pattern could impair the movement of fresh air to the respiratory tract of heifers, thus impacting the main mechanism for dissipating heat. The difference in temperature from the trailer ceiling to the animal level was 3.38°C in the trailer with lower porosity (cooler at the ceiling) and 2.23°C in the trailer with the higher porosity. This relationship also had a compartment location effect that followed the micro-climate compartmental differences. This could suggest that excess heat in the trailer with the lower porosity, that also had lower overall temperatures, exited through roof hatches, while in the trailer with the higher porosity, the heat escaped through the side-wall perforations. This theory also supports the idea that the location of where the porosity is located on the trailer may be important to alleviating heat stress in summer months during transport. The results of this study also indicated that there was no difference in the location of the data logger plane (driver, middle passenger) and within the compartments (front, middle, back), suggesting that compartment location effect is substantial when considering micro-climate but temperatures within a compartment are mostly homogenous. The trip that had average ambient temperatures of 25.9 ± 6.06°C for the entire journey, had a temperature Humidity Index that was considered in the danger or emergency category according to the Livestock Weather Heat Index during 95% of the warmest in-transit hour. This suggests that during ambient temperatures of 25.9°C, both trailers used in this study did not have sufficient heat exchange to mitigate the risk of heat stress for cattle.

Endocytosis effectuates a critical interface between the eukaryotic cell and its apposing environment. It is, subsequently, paramount for many physiologically important processes and encompasses a diverse array of mechanisms and pathways. The classical endocytic routes mediated by clathrin and caveolin are the best understood and the molecular roles of their major regulators, such as dynamin, adaptor proteins and various lipid species, are the most comprehensively described. Recent identification of an assortment of constitutive, noncaveolar, clathrin-independent endocytic (CIE) pathways has expanded the endocytic system. Unlike the classical endocytic pathways, little is known about the guiding parameters of CIE routes. Consequently, it is not possible to understand the important cellular roles these pathways may be fulfilling. This study has begun to characterise the very basic parameters governing the morphologically striking Clathrin-Independent Carrier (CLIC) pathway. Development of a diverse molecular toolkit has now allowed the quantitation of endocytic capacity provided by CLICs, the visualisation of subtle sorting components of the CLIC pathway, the isolation of novel CLIC cargo and regulators, and has linked this mechanism to the critical cellular processes of cellular migration and membrane repair. Calculation of the individual capacity of endocytic routes provides important information about the contribution of each pathway to total plasma membrane (PM) uptake and turnover. Quantitation of the volume, surface area and number of structures forming per minute in this study shows that CLICs provide the vast majority of constitutive endocytosis, up to four times the capacity of the clathrin mediated endocytic (CME) pathway. As the equivalent of the entire PM area could pass through the CLIC pathway within 12 minutes it is evident that CLICs are fundamental housekeepers of bulk membrane internalisation. Thus, they are likely to be central regulators of PM homeostasis and turnover. High-resolution tomography, in conjunction with analysis of CLIC cargo trafficking, identifies these carriers as complex, pleiomorphic structures that sort the bulk of membrane to early endosomes and recycle cargo back to the cell surface. Such vast internalisation combined with an ability to rapidly recycle components quickly attributes the CLIC pathway as a complex sorting station. Isolation of novel cargo and regulators has identified a striking array of proteins now associated with the CLIC pathway for the first time. A significant proportion of identified targets localise to lipid-rafts and recycle from the PM, facets consistent with association to the CLIC pathway. Numerous targets have also been directly implicated in clathrin-independent endocytosis by independent groups. Verification of selected cargo, such as CD44, Thy-1 and myoferlin, showing specific internalisation through the CLIC pathway, has provided insight into the sorting ability of the CLIC pathway and links to adhesion turnover and membrane recycling. Consistent with a role in cellular adhesion turnover, it was found that CLICs become polarised within migrating cells. This has shown the first instance of spatial separation between three major endocytic routes, CLICs, caveolae and CME and highlights the important and coordinated roles of multiple endocytic pathways during physiologically significant processes. The specific internalisation of paxillin, Thy-1 and CD44 through CLICs at the leading edge of migrating cells suggests that CLICs rapidly turnover adhesion components for dynamic extracellular sensation during directional cell migration. Indeed, specific ablation of the CLIC pathway significantly impedes cellular migration, implying coordination with CME at the leading edge. This study has defined numerous parameters of the CLIC pathway, developing the current understanding of this poorly defined route and places the CLIC pathway as a unique player during critical cellular processes.

Endocytosis effectuates a critical interface between the eukaryotic cell and its apposing environment. It is, subsequently, paramount for many physiologically important processes and encompasses a diverse array of mechanisms and pathways. The classical endocytic routes mediated by clathrin and caveolin are the best understood and the molecular roles of their major regulators, such as dynamin, adaptor proteins and various lipid species, are the most comprehensively described. Recent identification of an assortment of constitutive, noncaveolar, clathrin-independent endocytic (CIE) pathways has expanded the endocytic system. Unlike the classical endocytic pathways, little is known about the guiding parameters of CIE routes. Consequently, it is not possible to understand the important cellular roles these pathways may be fulfilling. This study has begun to characterise the very basic parameters governing the morphologically striking Clathrin-Independent Carrier (CLIC) pathway. Development of a diverse molecular toolkit has now allowed the quantitation of endocytic capacity provided by CLICs, the visualisation of subtle sorting components of the CLIC pathway, the isolation of novel CLIC cargo and regulators, and has linked this mechanism to the critical cellular processes of cellular migration and membrane repair. Calculation of the individual capacity of endocytic routes provides important information about the contribution of each pathway to total plasma membrane (PM) uptake and turnover. Quantitation of the volume, surface area and number of structures forming per minute in this study shows that CLICs provide the vast majority of constitutive endocytosis, up to four times the capacity of the clathrin mediated endocytic (CME) pathway. As the equivalent of the entire PM area could pass through the CLIC pathway within 12 minutes it is evident that CLICs are fundamental housekeepers of bulk membrane internalisation. Thus, they are likely to be central regulators of PM homeostasis and turnover. High-resolution tomography, in conjunction with analysis of CLIC cargo trafficking, identifies these carriers as complex, pleiomorphic structures that sort the bulk of membrane to early endosomes and recycle cargo back to the cell surface. Such vast internalisation combined with an ability to rapidly recycle components quickly attributes the CLIC pathway as a complex sorting station. Isolation of novel cargo and regulators has identified a striking array of proteins now associated with the CLIC pathway for the first time. A significant proportion of identified targets localise to lipid-rafts and recycle from the PM, facets consistent with association to the CLIC pathway. Numerous targets have also been directly implicated in clathrin-independent endocytosis by independent groups. Verification of selected cargo, such as CD44, Thy-1 and myoferlin, showing specific internalisation through the CLIC pathway, has provided insight into the sorting ability of the CLIC pathway and links to adhesion turnover and membrane recycling. Consistent with a role in cellular adhesion turnover, it was found that CLICs become polarised within migrating cells. This has shown the first instance of spatial separation between three major endocytic routes, CLICs, caveolae and CME and highlights the important and coordinated roles of multiple endocytic pathways during physiologically significant processes. The specific internalisation of paxillin, Thy-1 and CD44 through CLICs at the leading edge of migrating cells suggests that CLICs rapidly turnover adhesion components for dynamic extracellular sensation during directional cell migration. Indeed, specific ablation of the CLIC pathway significantly impedes cellular migration, implying coordination with CME at the leading edge. This study has defined numerous parameters of the CLIC pathway, developing the current understanding of this poorly defined route and places the CLIC pathway as a unique player during critical cellular processes.

Thesis (D.Phil.(Biokinetics, Sport and Leisure Sciences))--University of Pretoria, 2008. / Summary in English and Afrikaans. Includes bibliographical references.

Chronic exertional compartment syndrome (CECS) is a common problem within both military and athletic populations that can be difficult to diagnose. Furthermore, it is unclear what causes the development of CECS, particularly in the military population, as personnel undertake a variety of activities that can cause pain with CECS such as fast walking, marching and running. Chronic exertional compartment syndrome has been hypothesised to develop due to excessive muscle activity, foot pronation and abnormal biomechanics predominantly at the ankle. Treatment of CECS through running re-education to correct these abnormalities has been reported to improve symptoms. However no primary research has been carried out to investigate the biomechanical, muscle activation and clinical characteristics of military patients with CECS. The purpose of this thesis was to provide an original contribution to the knowledge through the exploration of these characteristics; and the development of insights into the development of CECS, with implications for prevention and treatment. Study one investigated the clinical characteristics of 93 service personnel with CECS. Plantar pressure variables, related to foot type and anterior compartment muscle activity, and ankle joint mobility were compared during walking between 70 cases and 70 controls in study two. Study three compared three-dimensional whole body kinematics, kinetics and lower limb muscle activity during walking and marching between 20 cases and 20 controls. Study four compared kinematics and lower limb muscle activity during running in a separate case-control cohort (n=40). Differences in electromyography (EMG) intensity during the gait cycle were compared in the frequency and time domain using wavelet analysis. All studies investigated subject anthropometry. Cases typically presented with bilateral, ‘tight’ or ‘burning’ pain in the anterior and lateral compartments of the lower leg that occurred within 10 minutes of exercise. This pain stopped all cases from exercising during marching and/or running. As such subsequent studies investigated the biomechanics of both ambulatory and running gaits. Cases in all case-control studies were 2-10 cm shorter; and were typically overweight resulting in a higher body mass index (BMI) than controls. There was strong evidence from study 3 that cases had greater relative stride lengths than controls during marching gait. This was achieved through an increase in ankle plantarflexion during late stance and a concomitant increase in the gastrocnemius medialis contraction intensity within the medium-high frequency wavelets. Given the differences in height observed, this may reflect ingrained alterations in gait resulting from military training; whereby all personnel are required to move at an even cadence and speed. These differences in stride length were also observed in walking and running gaits although to a lesser extent. There was no evidence from the EMG data that cases had greater tibialis anterior activation than controls during any activity tested, at any point in the gait cycle or in any frequency band. In agreement, there was also no evidence of differences between groups in plantar pressure derived measures of foot type, which modulate TA activity. Toe extensor - related plantar pressure variables also did not differ between groups. In summary, contrary to earlier theories, increased muscle activity of the anterior compartment musculature does not appear to be associated with CECS. The kinematic differences observed during running only partially matched the clinical observations previously described in the literature. Cases displayed less anterior trunk lean and less anterior pelvic tilt throughout the whole gait cycle and a more upright shank inclination angle during late swing (peak mean difference 3.5°, 4.1° and 7.3° respectively). However, no consistent differences were found at the ankle joint suggesting that running is unlikely to be the cause of CECS in the military; and that the reported success of biomechanical interventions may be due to reasons other than modifying pathological aspects of gait. In summary, the data presented in the thesis suggest that CECS is more likely to develop in subjects of shorter stature and that this is associated with marching at a constant speed and cadence. Biomechanical interventions for CECS, such as a change in foot strike or the use of foot orthotics, are unlikely to be efficacious for the military as personnel will continue to be required to march at prescribed speeds to satisfy occupational requirements. Preventative strategies that allow marching with a natural gait and/or at slower speeds may help reduce the incidence of CECS. The lack of association with foot type or muscle activity suggests that foot orthoses would not be a useful prevention strategy or treatment option for this condition.

L’écoulement de refroidissement, qui pénètre par les entrées d’air sous le capot des véhicules automobiles,est étudié à partir de trois approches complémentaires : les approches théorique, expérimentale et numérique. Ces trois approches s’appuient sur une maquette simplifiée à culot droit basée sur le corps de Ahmed et équipée d’un compartiment moteur. Les mesures expérimentales montrent que placer la sortie du compartiment moteur au culot de la maquette est plus favorable à une faible traînée de refroidissement qu’une sortie placée au niveau du soubassement. La contribution de la traînée de refroidissement dans la traînée totale peut ainsi varier de 2% à 24%. Les simulations numériques donnent elles accès au débit de refroidissement. Pour les configurations étudiées, les sorties au culot sont là encore les plus favorables puisqu’elles fournissent les débits les plus importants à travers le milieu poreux modélisant un échangeur aérothermique. Par ailleurs, la mise en place d’étanchéités de part et d’autre de l’échangeur améliore significativement le débit de refroidissement où une augmentation d’environ 45% est obtenue. La mise en place d’un modèle analytique permet de relier la traînée et le débit de refroidissement à partir d’une analogie entre les circuits électriques et les écoulements fluides. Il est alors possible de prévoir le sens d’évolution du débit de refroidissement, donc de la performance des échangeurs aérothermiques, à partir d’une modification géométrique interne au compartiment moteur. / The cooling airflow, which flows through the underhood of motor vehicles from the inlet sections, is studied by means of three complementary approaches: the theoretical, experimental and numerical approaches. The three approaches use a simplified geometry with a blunt rear end, based on the Ahmed body, and equipped with an engine compartment. The experimental measurements show that locating the outlet section of the engine compartment at the base of the geometry favors low cooling drag values compared to an outlet section located in the underbody. The variation of the cooling drag contribution in the total drag is between2% and 24%, as a function of the location of the outlet. As for the numerical simulations, they provide the cooling flow rates. For the studied configurations, rear end outlets are again the most favorable since they provide the highest flow rates through the porous media that simulates a heat exchanger. Besides, the implementation of sealing above and below the porous media significantly increases the cooling flow rate by45%. An analytical model, based on the analogy between electrical circuits and fluid flows, connects the aerodynamic drag with the cooling flow rate. It is then possible to predict the evolution trend of the cooling flow rate, hence the heat exchangers efficiency, from a geometrical modification inside the engine compartment.

Este trabalho apresenta uma variação do problema de roteamento de veículos que permite o uso de veículos com múltiplos compartimentos. A necessidade de veículos com múltiplos compartimentos surge com frequência em aplicações práticas quando uma série de produtos, que possuem diferentes qualidades ou tipo, precisam ser transportados mas não podem ser misturados. Este problema é chamado na literatura de roteamento de veículos com múltiplos compartimentos (PRVMC). Nós propomos uma heurística busca tabu implementada em uma busca local iterada para resolver este problema. Experimentos foram feitos para avaliar a performance da busca tabu iterada e os resultados obtidos foram comparados com os resultados disponíveis na literatura. O algoritimo proposto é capaz de encontrar soluções melhores e em menos tempo de processamento que as heurísticas existentes. / We study a variant of the vehicle routing problem that allows vehicles with multiple compartments. The need for multiple compartments frequently arises in practical applications when there are several products of different quality or type, that must be kept or handled separately. The resulting problem is called the multi-compartment vehicle routing problem (MCVRP). We propose a tabu search heuristic and embed it into an iterated local search to solve the MCVRP. In several experiments we analyze the performance of the iterated tabu search and compare it with results from the literature. We find that it consistently produces solutions that are better than existing heuristic algorithms.