Este trabalho apresenta uma variação do problema de roteamento de veículos que permite o uso de veículos com múltiplos compartimentos. A necessidade de veículos com múltiplos compartimentos surge com frequência em aplicações práticas quando uma série de produtos, que possuem diferentes qualidades ou tipo, precisam ser transportados mas não podem ser misturados. Este problema é chamado na literatura de roteamento de veículos com múltiplos compartimentos (PRVMC). Nós propomos uma heurística busca tabu implementada em uma busca local iterada para resolver este problema. Experimentos foram feitos para avaliar a performance da busca tabu iterada e os resultados obtidos foram comparados com os resultados disponíveis na literatura. O algoritimo proposto é capaz de encontrar soluções melhores e em menos tempo de processamento que as heurísticas existentes. / We study a variant of the vehicle routing problem that allows vehicles with multiple compartments. The need for multiple compartments frequently arises in practical applications when there are several products of different quality or type, that must be kept or handled separately. The resulting problem is called the multi-compartment vehicle routing problem (MCVRP). We propose a tabu search heuristic and embed it into an iterated local search to solve the MCVRP. In several experiments we analyze the performance of the iterated tabu search and compare it with results from the literature. We find that it consistently produces solutions that are better than existing heuristic algorithms.

Endocytosis effectuates a critical interface between the eukaryotic cell and its apposing environment. It is, subsequently, paramount for many physiologically important processes and encompasses a diverse array of mechanisms and pathways. The classical endocytic routes mediated by clathrin and caveolin are the best understood and the molecular roles of their major regulators, such as dynamin, adaptor proteins and various lipid species, are the most comprehensively described. Recent identification of an assortment of constitutive, noncaveolar, clathrin-independent endocytic (CIE) pathways has expanded the endocytic system. Unlike the classical endocytic pathways, little is known about the guiding parameters of CIE routes. Consequently, it is not possible to understand the important cellular roles these pathways may be fulfilling. This study has begun to characterise the very basic parameters governing the morphologically striking Clathrin-Independent Carrier (CLIC) pathway. Development of a diverse molecular toolkit has now allowed the quantitation of endocytic capacity provided by CLICs, the visualisation of subtle sorting components of the CLIC pathway, the isolation of novel CLIC cargo and regulators, and has linked this mechanism to the critical cellular processes of cellular migration and membrane repair. Calculation of the individual capacity of endocytic routes provides important information about the contribution of each pathway to total plasma membrane (PM) uptake and turnover. Quantitation of the volume, surface area and number of structures forming per minute in this study shows that CLICs provide the vast majority of constitutive endocytosis, up to four times the capacity of the clathrin mediated endocytic (CME) pathway. As the equivalent of the entire PM area could pass through the CLIC pathway within 12 minutes it is evident that CLICs are fundamental housekeepers of bulk membrane internalisation. Thus, they are likely to be central regulators of PM homeostasis and turnover. High-resolution tomography, in conjunction with analysis of CLIC cargo trafficking, identifies these carriers as complex, pleiomorphic structures that sort the bulk of membrane to early endosomes and recycle cargo back to the cell surface. Such vast internalisation combined with an ability to rapidly recycle components quickly attributes the CLIC pathway as a complex sorting station. Isolation of novel cargo and regulators has identified a striking array of proteins now associated with the CLIC pathway for the first time. A significant proportion of identified targets localise to lipid-rafts and recycle from the PM, facets consistent with association to the CLIC pathway. Numerous targets have also been directly implicated in clathrin-independent endocytosis by independent groups. Verification of selected cargo, such as CD44, Thy-1 and myoferlin, showing specific internalisation through the CLIC pathway, has provided insight into the sorting ability of the CLIC pathway and links to adhesion turnover and membrane recycling. Consistent with a role in cellular adhesion turnover, it was found that CLICs become polarised within migrating cells. This has shown the first instance of spatial separation between three major endocytic routes, CLICs, caveolae and CME and highlights the important and coordinated roles of multiple endocytic pathways during physiologically significant processes. The specific internalisation of paxillin, Thy-1 and CD44 through CLICs at the leading edge of migrating cells suggests that CLICs rapidly turnover adhesion components for dynamic extracellular sensation during directional cell migration. Indeed, specific ablation of the CLIC pathway significantly impedes cellular migration, implying coordination with CME at the leading edge. This study has defined numerous parameters of the CLIC pathway, developing the current understanding of this poorly defined route and places the CLIC pathway as a unique player during critical cellular processes.

<p>Ambulanssjukvård bedrivs både på hämtplats och under transporten till mottagande enhet. Detta medför att personalen utför vårdåtgärder i vårdutrymmet under transporten enligt tidigare forskning sker detta ofta utan att personalen använder sig av bilbältet.</p><p>Syftet med denna studie var att undersöka ambulanspersonalens upplevelser av trafiksäkerheten i vägburna ambulanser.</p><p>Metoden har varit intervjuer med semistrukturerade frågor samt en öppen fråga. Intervjuerna har genomförts med ambulanspersonalen i deras arbetsmiljö, det vill säga ambulansens vårdutrymme. Intervjuerna har sammanställts och en innehållsanalys har gjorts på den öppna frågan.</p><p>Resultatet visar att personalen inte använder säkerhetsutrustningen i den utsträckning som de borde och vill göra. Arbetsmiljön och patientens tillstånd var helt avgörande. Personalen satte sin egen säkerhet åt sidan och fokuserade helt på patienten och dennes behov. Personalen upplevde dock att patienterna var trygga i vårdutrymmet.</p><p>Enligt flera forskare så har problemet med bilbältesanvändning i vårdutrymmet uppmärksammats under flera år. Men inga nya metoder eller idéer har anammats. Denna studies resultat förväntas öka förståelsen för den problematik som finns med att arbeta i ambulansens vårdutrymme på ett för personalen säkert sätt.</p> / <p>Prehospital care is carried out on the scene and during transport to a reciving unit. Thus, the ambulance staff is performing care assignment in the patient compartment during transport. According to prior research, the care is often made by the attending personal not using the seatbelts.</p><p>The aim of this study was to examine the ambulance staff´s experiences of traffic saftey in the patient compartment in a road ambulance.</p><p>The method used was semi structured interviews and one open question. The interviews have been carried out in the ambulance staff own working environment, the patient compartment. The interviews were put together and the open question was analysed by means of a qualitative content analysis.</p><p>The result shows that the ambulance staff did not use the saftey equipment in the patient compartment to the extent they should and whished to do. The working environment and the patient´s condition were the reason for not using seatbelts. The ambulance staff put their own saftey a side, and focused completley on the patient and his or her needs. However, the ambulance staff felt that the patient was safe in the patient compartment.</p><p>Several studies have previously shown that there is a problem with the lack of use of seatbelts in the patient compartment. However, no new ideas or method for consistent use of seatbelts has been adopted. The result of this study should increase awareness for the complex problems concerning work saftey for ambulance staff in the patient compartment.</p>

Ambulanssjukvård bedrivs både på hämtplats och under transporten till mottagande enhet. Detta medför att personalen utför vårdåtgärder i vårdutrymmet under transporten enligt tidigare forskning sker detta ofta utan att personalen använder sig av bilbältet. Syftet med denna studie var att undersöka ambulanspersonalens upplevelser av trafiksäkerheten i vägburna ambulanser. Metoden har varit intervjuer med semistrukturerade frågor samt en öppen fråga. Intervjuerna har genomförts med ambulanspersonalen i deras arbetsmiljö, det vill säga ambulansens vårdutrymme. Intervjuerna har sammanställts och en innehållsanalys har gjorts på den öppna frågan. Resultatet visar att personalen inte använder säkerhetsutrustningen i den utsträckning som de borde och vill göra. Arbetsmiljön och patientens tillstånd var helt avgörande. Personalen satte sin egen säkerhet åt sidan och fokuserade helt på patienten och dennes behov. Personalen upplevde dock att patienterna var trygga i vårdutrymmet. Enligt flera forskare så har problemet med bilbältesanvändning i vårdutrymmet uppmärksammats under flera år. Men inga nya metoder eller idéer har anammats. Denna studies resultat förväntas öka förståelsen för den problematik som finns med att arbeta i ambulansens vårdutrymme på ett för personalen säkert sätt. / Prehospital care is carried out on the scene and during transport to a reciving unit. Thus, the ambulance staff is performing care assignment in the patient compartment during transport. According to prior research, the care is often made by the attending personal not using the seatbelts. The aim of this study was to examine the ambulance staff´s experiences of traffic saftey in the patient compartment in a road ambulance. The method used was semi structured interviews and one open question. The interviews have been carried out in the ambulance staff own working environment, the patient compartment. The interviews were put together and the open question was analysed by means of a qualitative content analysis. The result shows that the ambulance staff did not use the saftey equipment in the patient compartment to the extent they should and whished to do. The working environment and the patient´s condition were the reason for not using seatbelts. The ambulance staff put their own saftey a side, and focused completley on the patient and his or her needs. However, the ambulance staff felt that the patient was safe in the patient compartment. Several studies have previously shown that there is a problem with the lack of use of seatbelts in the patient compartment. However, no new ideas or method for consistent use of seatbelts has been adopted. The result of this study should increase awareness for the complex problems concerning work saftey for ambulance staff in the patient compartment.

Noise-like activity (NLA) refers to spontaneous subthreshold fluctuations in membrane potential. In this thesis, we examine the role that synaptic channel fluctuations play in contributing to NLA by comparing a detailed biophysical model to experimental data from whole-intact hippocampal interneurons. To represent the contribution from synaptic channel fluctuations, we switch the synapses in the model from traditional to Markovian formalisms and demonstrate statistically relevant increases the standard deviation; power-law scaling exponent; and power spectral density in the 5-100 Hz and 1-5 kHz ranges. However, while synaptic channel fluctuations have a definite effect, we found that they were significantly more subtle than the synaptic response to network activity. This indicates that synaptic channel fluctuations do indeed play a significant role in subthreshold noise, but, overall, synaptic NLA is dominated by the synaptic response to presynaptic network activity.

Noise-like activity (NLA) refers to spontaneous subthreshold fluctuations in membrane potential. In this thesis, we examine the role that synaptic channel fluctuations play in contributing to NLA by comparing a detailed biophysical model to experimental data from whole-intact hippocampal interneurons. To represent the contribution from synaptic channel fluctuations, we switch the synapses in the model from traditional to Markovian formalisms and demonstrate statistically relevant increases the standard deviation; power-law scaling exponent; and power spectral density in the 5-100 Hz and 1-5 kHz ranges. However, while synaptic channel fluctuations have a definite effect, we found that they were significantly more subtle than the synaptic response to network activity. This indicates that synaptic channel fluctuations do indeed play a significant role in subthreshold noise, but, overall, synaptic NLA is dominated by the synaptic response to presynaptic network activity.

This thesis reports on the procedure, results and analysis of four full scale fire tests that were performed at the University of Waterloo's Live Fire Research Facility. The purpose of these tests was to investigate the thermal characteristics of one room of the Burn House structure. Comparisons were made of Burn House experimental data to previous residential fire studies undertaken by researchers from the University of Waterloo. This analysis showed similarities in growth rate characteristics, illustrating that fire behaviour in the Burn House is typical of residential structure fire behaviour. The Burn House experimental data was also compared to predictions from a fire model, CFAST. Recommendations were made for future work in relation to further investigation of the fire characteristics of the Burn House.

The objective of the present study was to explore the correlation between resting metabolic rate (RMR), body composition and physical activity in active and inactive men and women aged 20-30 years. In total, 13 active and 10 inactive women and 8 active and 5 inactive men were enrolled in this study. RMR was measured using an indirect respiratory calorimeter and body composition was obtained using a BodPod, anthropometric measurements (measurements of waist, weight, skin fold thickness etc.) and bioelectrical impedance analysis. From the BodPod were information obtained about fat-free mass (FFM) and fat mass (FM) and from the bioelectrical impedance analysis were data obtained about the total body water (TBW). By combining the data from these methods a three-compartment model could be produced. This gives a better value of the body composition. By using anthropometric measurements the muscle mass (MM) could be calculated. FFM measurements correlate best with RMR for both active men and women and for inactive women. For the inactive men MM correlates best with RMR. In conclusion, comparing the groups as a whole it is seen that the FFM correlates best with RMR for both women and men. Since the muscles are the metabolically active part of FFM it is concluded that muscles affects the RMR value and muscles can in turn be influenced by training.

This thesis reports on the procedure, results and analysis of four full scale fire tests that were performed at the University of Waterloo's Live Fire Research Facility. The purpose of these tests was to investigate the thermal characteristics of one room of the Burn House structure. Comparisons were made of Burn House experimental data to previous residential fire studies undertaken by researchers from the University of Waterloo. This analysis showed similarities in growth rate characteristics, illustrating that fire behaviour in the Burn House is typical of residential structure fire behaviour. The Burn House experimental data was also compared to predictions from a fire model, CFAST. Recommendations were made for future work in relation to further investigation of the fire characteristics of the Burn House.

Introduktion: Omvårdnad är operationssjuksköterskans huvudområde. Operationssjuksköterskan ska förebygga skador och komplikationer som kan uppstå vid en operation. En sådan komplikation kan vara kompartmentsyndrom. Kompartmentsyndrom är en ovanlig komplikation men kan leda till allvarliga skador. Det är därför viktigt att operationssjuksköterskan beaktar det i sitt arbete. Syfte: Detta arbete syftar till att belysa operationssjuksköterskans erfarenheter av att arbeta med att förebygga kompartmentsyndrom i den perioperativa omvårdnaden. Metod: En kvalitativ ansats tillämpades och data samlades in via intervjuer. Insamlade data analyserades genom kvalitativ innehållsanalys. I arbetet deltog 11 operationssjuksköterskor, tio kvinnor och en man, som arbetade på fyra olika operationsavdelningar vid två olika sjukhus. Resultat: I den kvalitativa innehållsanalysen av texten framkom två kategorier, Att arbeta förebyggande samt Att handla på osäker grund. I den förstnämnda kategorin beskrevs hur operationssjuksköterskorna genomförde en individuell riskbedömning, var noggranna i positioneringen och utförde lägesändring samt massage av patientens ben för att förebygga kompartmentsyndrom. I den andra kategorin, Att handla på osäker grund, beskrevs en osäkerhet i den perioperativa omvårdnaden kring kompartmentsyndrom. Det belystes en osäkerhet runt ämnet, omvårdnadsinterventionerna ifrågasattes och en brist på vetenskaplig evidens framkom. Konklusion: Det fanns brister i riktlinjer och rutiner gällande omvårdnad kring kompartmentsyndrom. Det fanns även en brist i att implementera ny kunskap i operationssjuksköterskans omvårdnad för att kunna arbeta evidensbaserat. / Introduction: Nursing is the main area for the operating room (OR) nurse. The OR nurse will prevent injuries and complications that can occur during surgery. One such complication can be compartment syndrome. Compartment syndrome is a rare complication but can lead to serious injuries. It is therefore important that the OR nurse take this in consideration in their work. Aim: This essay aims to highlight the OR nurse's experiences of working with the prevention of compartment syndrome in the perioperative nursing. Method: A qualitative approach was applied and the data were collected through interviews. Collected data were analyzed by qualitative content analysis. The essay enrolled 11 OR nurses, ten women and one man, who worked in four different surgical wards at two different hospitals. Result: After a qualitative content analysis of the data were conducted two categories emerged, Preventive work and To act on uncertain foundations. The first category described how the OR nurse undertook an individual risk assessment, was accurate in positioning and performed a change in position and massage of the patient’s leg to prevent compartment syndrome. The second category, To act on uncertain foundations, described an uncertainty in the perioperative care regarding compartment syndrome. It highlighted an uncertainty around the topic, nursing interventions were questioned and a lack of scientific evidence emerged. Conclusion: There were a lack of guidelines and recommendations regarding care about compartment syndrome. There was also a lack of implementing new knowledge in nursing to provide an evidence-based practice.