Helical membrane proteins comprise one third of the expressed proteins encoded in a typical genome. Other membrane proteins are typically beta sheets. Their function varies from pore formation, signaling to antimicrobial activity. They are also capable of transporting large cargo such as proteins or nucleic acids across the cell membrane. Recently, peptides have emerged as promising tools in drug delivery. Membrane proteins can be synthesized chemically or expressed and isotopically labeled in bacteria, isolated, purified and reconstituted into fully hydrated lipid bilayers. The bilayer orientation is kept mechanically by putting them between glass plates. While interacting with these bilayers they exhibit a variety of configurations depending on the lipids composition and thickness. Solid-state Nuclear Magnetic Resonance (NMR) on oriented bilayers is one way to access the topology of peptides associated with phospholipid membranes. Oriented membrane protein are difficult to study with analytical techniques because of their poor solubility outside the lipid membrane, difficulty of expression in bacteria in big quantities, difficulty to crystallize, and they are too large for solution NMR study. The intensity of an NMR signal depends on several factors such as polarization P and magnetic field magnitude B0. One of the major drawbacks of NMR spectroscopy is low sensitivity. This is caused by the small magnetic moment of the nuclear spins which results in a modest Zeeman splitting of the nuclear spin energy levels and therefore in a limited Boltzmann Polarization. The aim of this project is to obtain a better signal from membrane proteins. Thus a Low temperature (LT) solid state NMR with Dynamic Nuclear Polarization (DNP) probe head was created. DNP is an ingenious technique that is used to transfer polarization from highly polarized targets to less polarized nuclei using microwave irradiation. Microwaves will excite selectively the electron spins which will transfer their polarization to the pool of proton nuclei, the proton NMR signal can be enhanced by 660 times. A probe head for DNP enhanced solid state NMR at 100 K and 9.4 T is described. A probe head includes the mechanical piece that holds the sample in the magnetic center of the NMR magnet. It is a tunable antenna that irradiates and detects the rf fields used in NMR. The centerpiece of the probe is the solenoidal or saddle coil surrounding the sample. The feasibility of such a DNP experiment is proven on magic angle oriented sample spinning. These experiments are conducted on oriented samples wrapped into a rotor. Through their orientation with regards to B0 is lost, enhancement values as high as 17 are obtained. [...]

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Les protéines membranaires en hélices alpha forment le tiers des protéines codées par notre génome. D’autres protéines membranaires sont formées typiquement de feuillets bêta. Leur fonction varie de la formation de pores, la transmission de signaux à l’activité antibiotique. Elles sont aussi capables de transporter de larges cargos comme les protéines ou les acides nucléiques au travers de la membrane. Récemment, les peptides ont émergé comme un moyen prometteur pour le transport de médicaments vers leurs cibles.Les protéines membranaires peuvent être synthétisées chimiquement ou exprimées et marquées isotopiquement dans les bactéries, isolées, purifiées et reconstituées dans les bicouches lipidiques hydratées. Elles présentent une variété de configurations en interagissant avec ces bicouches lipidiques. Ceci dépend de la composition et de l’épaisseur de ces bicouches. L’orientation des bicouches lipidiques est maintenue mécaniquement en les disposant entre des plaques de verre. La RMN du solide des échantillons orientés est un des moyens possibles pour accéder à la topologie des peptides associés à des membranes phospholipidiques. Les échantillons sont difficiles à exprimer dans les bactéries en grande quantités et possède une solubilité réduite en dehors des membranes. En outre leur taille est trop importante pour la RMN du liquide et il est difficile de les cristalliser. Un des inconvénients majeur de la spectroscopie RMN est sa faible sensibilité. Cela résulte du faible moment magnétique nucléaire qui résulte en un décalage Zeeman faible et donc une polarisation réduite. Par ailleurs, l’intensité du signal RMN dépend de plusieurs facteurs comme la quantité d’échantillon la polarisation et le champ magnétique B0. Et le temps d’acquisition de certaines expériences peut être très long. Le but de ce projet est d’obtenir plus de signal des protéines membranaires. Dès lors, nous avons développé une cryosonde DNP (dynamic nuclear polarization) / RMN du solide. La DNP est une technique ingénieuse qui est utilisée pour le transfert de polarisation des noyaux hautement polarisés à des noyaux moins polarisés par irradiation microonde. Les microondes vont exister sélectivement les électrons qui transfèreront leur polarisation à l’ensemble des protons voisins, le signal proton peut ainsi être augmenté de 660 fois.Pour cela la cryosonde DNP RMN du solide qui opère à 100 K et 9,4 T a été utilisée. Une sonde est la pièce mécanique qui maintient l’échantillon dans le centre magnétique de l’aimant du spectromètre. C’est une antenne modulable qui irradie et détecte des champs radiofréquence. La pièce centrale de la sonde est une bobine solénoïdale ou une bobine en forme de selle enveloppant l’échantillon. La faisabilité de ces expériences DNP a été validée sur les échantillons orientés en rotation à l’angle magique. Ces expériences ont été menées sur des échantillons enroulés dans un rotor. Même si leur orientation par rapport au champ magnétique B0 est perdue, une valeur d’augmentation de 17 a été obtenue.[...] / Helical membrane proteins comprise one third of the expressed proteins encoded in a typical genome. Other membrane proteins are typically beta sheets. Their function varies from pore formation, signaling to antimicrobial activity. They are also capable of transporting large cargo such as proteins or nucleic acids across the cell membrane. Recently, peptides have emerged as promising tools in drug delivery. Membrane proteins can be synthesized chemically or expressed and isotopically labeled in bacteria, isolated, purified and reconstituted into fully hydrated lipid bilayers. The bilayer orientation is kept mechanically by putting them between glass plates. While interacting with these bilayers they exhibit a variety of configurations depending on the lipids composition and thickness. Solid-state Nuclear Magnetic Resonance (NMR) on oriented bilayers is one way to access the topology of peptides associated with phospholipid membranes. Oriented membrane protein are difficult to study with analytical techniques because of their poor solubility outside the lipid membrane, difficulty of expression in bacteria in big quantities, difficulty to crystallize, and they are too large for solution NMR study. The intensity of an NMR signal depends on several factors such as polarization P and magnetic field magnitude B0. One of the major drawbacks of NMR spectroscopy is low sensitivity. This is caused by the small magnetic moment of the nuclear spins which results in a modest Zeeman splitting of the nuclear spin energy levels and therefore in a limited Boltzmann Polarization. The aim of this project is to obtain a better signal from membrane proteins. Thus a Low temperature (LT) solid state NMR with Dynamic Nuclear Polarization (DNP) probe head was created. DNP is an ingenious technique that is used to transfer polarization from highly polarized targets to less polarized nuclei using microwave irradiation. Microwaves will excite selectively the electron spins which will transfer their polarization to the pool of proton nuclei, the proton NMR signal can be enhanced by 660 times. A probe head for DNP enhanced solid state NMR at 100 K and 9.4 T is described. A probe head includes the mechanical piece that holds the sample in the magnetic center of the NMR magnet. It is a tunable antenna that irradiates and detects the rf fields used in NMR. The centerpiece of the probe is the solenoidal or saddle coil surrounding the sample. The feasibility of such a DNP experiment is proven on magic angle oriented sample spinning. These experiments are conducted on oriented samples wrapped into a rotor. Through their orientation with regards to B0 is lost, enhancement values as high as 17 are obtained. [...]

Made available in DSpace on 2015-05-14T12:14:16Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 5113295 bytes, checksum: 299112e42f72744b732d195da1230c13 (MD5) Previous issue date: 2015-05-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / In this thesis we address issues pertaining to the fluctuations of the electromagnetic field in the optical domain, both experimentally as well as theoretically and numerically. It was experimentally demonstrated the interdependence between the degree of polarization and the degree of coherence of thermal optical fields. We analyzed the results and explained them in light of a new unifying theory. We have shown a way to control the coerence of light through polarization manipulations, concluding with a proposal for a simple thermal light source with a tunable spectral electromagnetic degree of coherence. We expanded the concept of a tunable light source, suggesting a partially coherent and partially polarized light source with complete control of the so called generalized Stokes parameters by mixing polarized stochastic optical fields. We obtain theoretically the statistical properties of the resulting optical field and their dependence on the properties of the primary fields. We discuss fundamental properties of the mixture of stochastic fields and point out some peculiarities through examples. All results are demonstrated through numerical simulations. We suggest a method to investigate the spatial statistical properties of gaussian stochastic fields through the Hanbury Brown-Twiss (HBT) interferometer, an admittedly simple experimenal method. We show theoretically that it is possible to measure the generalized Stokes parameters, thus allowing one to rebuild the coherence matrix and the polarization matrix of the field, completely characterizing the optical field both statistically and locally in the observation plane. We suggest a technique for the automation of the method, and also some applications. These many studies on the statistical properties of optical fields led us to a deeper understanding of the physical meanings of the parameters for characterizing them. From this understanding we also discuss a change to the definition of the cross-polarization degree and the significance of optical coherence in terms of this new definition. / Nesta tese abordamos problemas concernentes às flutuações do campo eletromagnético no domínio ótico, tanto experimentalmente quanto teoricamente e numericamente. Demonstramos experimentalmente a interdependência entre o grau de polarização e o grau de coerência de campos óticos térmicos. Analizamos os resultados e os explicamos à luz de novas teorias unificadoras. Mostramos assim uma forma de controlar a coerência da luz através de manipulações na polarização, concluindo com uma proposta de fonte de luz térmica simples, com grau espectral de coerência eletromagnética sintonizável. Expandimos o conceito de fonte luz sintonizável, propondo uma fonte de luz parcialmente coerente e parcialmente polarizada com completa liberdade de controle dos chamados parâmetros de Stokes generalizados, através da mistura de campos óticos estocásticos polarizados. Obtemos teoricamente as propriedades estatísticas do campo ótico resultante e a dependência destas com as propriedades dos campos primários. Discutimos propriedades fundamentais da mistura de campos estocásticos e apontamos algumas peculiaridades através de exemplos. Demostramos todos os resultados através de simulações numéricas. Sugerimos um método para investigar as propriedades estatísticas espaciais de campos estocásticos gaussianos através do interferômetro de Hanbury Brown-Twiss, um método experimenal reconhecidamente simples. Mostramos teoricamente que é possível medir os parâmetros de Stokes generalizados, permitindo reconstruir a matriz de coerência e a matriz de polarização do campo, caracterizando completamente o campo ótico tanto estatisticamente quanto localmente. Apontamos ainda técnica para a automação do método, além de aplicações. Os diversos estudos realizados acerca das propriedades estatísticas de campos óticos nos levaram a um entendimento profundo dos significados físicos dos parâmetros utilizados para caracterizá-los. A partir deste entendimento, discutimos ainda uma modificação na definição do grau de polarização cruzado e da significância da coerência ótica em termo desta nova definição.

Phased array weather radar antennas with beam steering capabilities are suitable alternatives to weather radars with mechanically scanning reflector antennas. Dual-polarized phased-array weather radar antennas, however, demand careful assessment of the x-polar characteristics. The low x-pol radiation of polarimetric weather radar antennas is of significant importance for the proper classification and qualitative estimation of hydrometeors in illuminate volumes. Unfortunately, array antennas display changing x-pol contributions during the electronical beam steering process. Typically, the x-pol radiation will be substantially increased in the co-polar main beam direction but also in other angular directions. Consequently, it is a vital challenge to design arrays with low x-pol contribution during beam steering. In this dissertation a new phased-array weather radar concept is developed. The phased array system configuration can be used to substitute state-of-the-art weather radars with reflector antennas. Furthermore, a dense network of these phased-array radars can be used to substitute a network of high power weather radars, which are used nowadays. The research focus of this work is the development of a dual-polarized microstrip patch antenna with phased-array capability and very high polarization purity. In this regard, new graphical techniques are developed to investigate the causes and the reduction of the x-pol radiation of isolated (stand-alone) microstrip patch antennas. To further reduce the x-pol contribution of antennas, optimization methods have been investigated, evaluated and developed. For the first time in literature, differential-feed antenna arrays are compared to excitation optimized single-feed antenna arrays in their x-pol contribution in the boresight direction and during beam steering. In particular, two dual-polarized 4x8 antenna arrays have been developed and simulated by CST MWS, produced as multilayer PCB and verified at the compact antenna test range at RWTH Aachen. The results show that the x-pol contributions of arrays are significantly reduced for differentially-feed antenna arrays, even when beam steering is performed. During the azimuth scan of 120_ a record setting x-pol suppression of -45 dB and -36 dB could be measured for the horizontal and vertical polarization channels, respectively. / Wetterradarsysteme mit phasengesteuerten Antennen stellen eine echte Alternative zu Wetterradarsystemen mit mechanisch drehenden Reflektorantennen dar. Dual-polarisierte phasengesteuerte Antennen müssen jedoch sehr genau in ihrem Kreuzpolarisationsverhalten verifiziert werden, um für den Wetterradarbereich von Nutzen zu sein. Die Unterdrückung der kreuzpolaren Anteile von Radarantennen ist von fundamentaler Bedeutung, um Hydrometeore mit Hilfe von polarimetrischen Wetterradarsystemen klassifizieren und qualitativ bestimmen zu können. Die hohe Anforderung an Polarisationsreinheit ist mit aktuell erhältlichen Arraydesigns nur schwierig zu realisieren, da sich die Kreuzpolarisationsunterdrückung während des elektronischen Schwenks der Hauptkeule signifikant verschlechtert. Diese Dissertation stellt ein Wetterradar Systemkonzept mit phasengesteuerter Gruppenantenne vor, welches die aktuell genutzten Wetterradare mit Reflektorantennen ablösen könnte. Der Fokus der Arbeit wurde auf die Entwicklung einer Dual-polarimetrischen, polarisationsreinen und phasengesteuerten Mikrostreifenleiterantennen gelegt. Hierbei wurden neue grafische Verfahren entwickelt, die es ermöglichen, die Generierung der kreuzpolaren Anteile von isolierten Patchantennen (Einzelpatche) zu erklären und zu minimieren. Um die kreuzpolaren Anteile weiter herabzusetzen wurden Optimierungsverfahren für Arrayantennen erforscht, bewertet und neu entwickelt. Zum ersten Mal wurden differentiell gespeiste mit einzeln gespeisten Antennenarrays in ihrem Kreuzpolarisationsverhalten während des elektronischen Schwenks der Hauptkeule verglichen. Zwei Dual- polarimetrische 4x8 Antennenarrays (differentiell gespeist und mit optimierter Phasenansteuerung) wurden zu diesem Zweck mittels CST MWS entworfen, simuliert, als Multilagenplatine gefertigt und an der Antennentestanlage der RWTH Aachen vermessen. Die Resultate zeigen, dass die Kreuzpolarisationsanteile bei differentiell gespeisten Mikrostreifenleiterantennen in Gruppenkonfiguration, selbst beim elektronischen Schwenk der Hauptkeule, signifikant minimiert werden konnten. Für einen azimutalen Scanbereich von 120_ konnte eine exzellente Kreuzpolarisationsunterdrückung zwischen -45 dB und -36 dB messtechnisch für den horizontalen und vertikalen Polarisationskanal nachgewiesen werden.

La résonance magnétique nucléaire (RMN) est désormais devenue une technique spectroscopique incontournable à de nombreux domaines de la science et de la médecine. Cependant, elle est limitée par une faible sensibilité en raison d'une polarisation nucléaire insuffisante, définie par la différence des populations entre les niveaux d'énergie impliqués, ce qui résulte à des temps expérimentaux assez longs. Cet inconvénient peut être compensé en transférant l’énorme polarisation des électrons non appariés aux spins nucléaires à l'aide d'une irradiation par micro-ondes à basse température - une méthode connue sous le nom de polarisation nucléaire dynamique (DNP). Sami Jannin et ses collègues ont récemment combiné la méthode de polarisation croisée (CP) avec la DNP, à des températures très basses, afin de polariser plus rapidement les noyaux de faible γ en transférant la polarisation des électrons aux protons et ensuite aux noyaux de faible γ, comme le carbone-13. Cette thèse démontre que l'efficacité de cette méthode peut être améliorée en arrêtant l'irradiation par micro-ondes pendant quelques centaines de millisecondes avant la CP. En effectuant ces expériences, la polarisation du carbone-13 de l'acétate de sodium [1-¹³C] peut monter jusqu'à 64% avec une constante de temps d'accumulation de polarisation très courte de 160 s. L'échantillon hyperpolarisé peut ensuite être rapidement dissous pour obtenir un signal RMN amplifié à l'état liquide et à la température ambiante. Cette méthode a été développée par Ardenkjaer-Larsen et ses collègues en 2003 et est connue sous le nom de polarisation nucléaire dynamique par dissolution (D-DNP). Elle peut fournir une amplification du signal d'un facteur jusqu'à quatre ordres de grandeur. La combinaison de la D-DNP avec les techniques de CP peut être utilisée pour créer des états de longue durée de vie (LLS) dans des molécules deutériées comme l'éthanol-d6 et le DMSO-d6. Ces états peuvent avoir des durées de vie beaucoup plus longues que l'aimantation conventionnelle de Zeeman. Ces états peuvent être détectés indirectement via des multiplets asymétriques dans les spectres RMN du carbone-13 en solution, à température ambiante après dissolution, via les couplages scalaires entre le carbone-13 et les noyaux du deutérium. Ces LLS résultent du déséquilibre des populations entre les représentations irréductibles des groupes de symétrie des molécules deutériées étudiées. Dans cette thèse, nous avons étudié les LLS dans les groupes CD₂ et CD₃. Les durées de vie de ces LLS peuvent dépasser les temps de relaxation T₁(²H) des noyaux de deutérium par un facteur jusqu'à 20 (dépendant de la dynamique moléculaire) et peuvent être observées par la décroissance de l'asymétrie du multiplet du carbone-13. Ces observations peuvent élargir les applications de la DNP en ajoutant le noyau de ²H à la liste des observables possibles / Nowadays, Nuclear Magnetic Resonance (NMR) has become an inevitable spectroscopic technique that can be applied in many fields of science and medicine. However it is limited by low sensitivity due to the low nuclear polarization, defined by the difference of populations between the energy levels involved, thus leading to long experimental times. This drawback can be overcome by using the huge polarization of unpaired electrons compared to nuclear spins and its transfer to nuclear spins at low temperatures (using microwave irradiation) to achieve a large nuclear magnetization - a method know as dynamic nuclear polarization (DNP). Sami Jannin and co-workers have recently combined the cross-polarization (CP) method with DNP at very low temperatures to polarize low γ nuclei faster by transferring the electron polarization to protons and then to low γ nuclei like ¹³C. This thesis demonstrates that the efficiency of the CP method can be improved by switching the microwave irradiation off for a few hundred milliseconds prior to CP. By performing these microwave gating experiments, ¹³C polarizations for sodium [1-¹³C]acetate as high as 64% could be achieved with a polarization build-up time constant as short as 160 s. The hyperpolarized sample can subsequently be rapidly dissolved to achieve NMR signal enhancement in the liquid state at ambient temperature. This method has been developed by Ardenkjaer-Larsen and co-workers in 2003 and became known as dissolution-dynamic nuclear polarization (D-DNP). It can provide signal enhancements by a factor of up to four orders of magnitude in liquid state at room temperature. Combining D-DNP with CP techniques from ¹H to ¹³C can be used to create to create long-lived states (LLS) in deuterated molecules like ethanol-d₆ and DMSO-d₆, which can have much longer life times than conventional Zeeman magnetization. These LLS can be detected indirectly via the asymmetric multiplets in ¹³C NMR spectra in solution state at room temperature after dissolution due to scalar couplings between ¹³C and ²H. These LLS result from population imbalances between spin manifolds of distinct irreducible representations of the relevant symmetry groups of the investigated deuterated moities. In this thesis we have investigated LLS in CD₂ and CD₃ groups. Lifetimes of these LLS can exceed the spin-lattice relaxation times T₁(²H) of deuterium nuclei by a factor up to 20 (depending on the underlying molecular dynamics) and can be obtained by monitoring the decay of the asymmetry of the ¹³C multiplet. These observations can expend the scope of DNP by adding ²H nucleus to the list of possible observables.

The Terrestrial digital radio links are used for transmitting digital information between fixed stations on a line of sight of a radio path. Each digital radio link has many characteristics and parameters for transmission. We require technical parameters in the design of digital radio link such as data rates and capacities. In this design we should take into account most effects of the electromagnetic waves propagation, if we want to meet user technical requirements of the link and if that link may correctly transmit digital signals with minimum errors. Prediction methods for the design of terrestrial line of sight systems are still in progress. Some effects of electromagnetic waves propagation and methods of the digital radio link design depend on natural variability and climate. The goal of this diploma thesis is to investigate methods for the design of terrestrial line of sight links with taking into account of ITU recommendation. Make computer program which implement this investigate methods

本研究は, 広帯域直交偏波共用低交差偏波特性, 偏波変換特性および任意の反射位相差を有する高性能・高機能リフレクトアレー共振素子の開発を行い, 設計・試作したリフレクトアレーアンテナの放射特性の数値的及び実験的検討の研究成果をまとめたものである. / 博士(工学) / Doctor of Philosophy in Engineering / 同志社大学 / Doshisha University