Les travaux exposés dans cette thèse sont le reflet d'années d'efforts consacrées à la mise en place de diagnostics de qualités des hépatites virales au Laos au sein du Centre d'infectiologie Lao Christophe Mérieux (CILM), dont je suis aujourd'hui la directrice scientifique. En introduction, nous avons tenu à présenter les caractéristiques socio-économiques du Laos et ses défis sanitaires pour atteindre les Millennium Development Goals, qui guident les politiques publiques de pays en situation de développement. Nous avons jugé important de souligner ces points tout comme la présentation du CILM pour faire comprendre au lecteur le contexte de réalisation de ce travail. C'est grâce à une bio-banque riche de plus de 7,000 échantillons biologiques de patients infectés par les virus des hépatites virales B et C, que nous avons pu poser la question de recherche clé de cette thèse : Comment évoluent les hépatites virales chez les patients reçus depuis une dizaine d'années au CILM ? Cette question en a fait émerger une deuxième concernant la qualité du diagnostic dans les centres de soins à Vientiane. Ainsi, nous nous sommes intéressés aux kits de diagnostic rapide de l'hépatite B utilisés dans les structures de soins de Vientiane, comparés au standard utilisé dans notre laboratoire. Ces travaux, qui ont donné lieu à la publication de 2 articles (un troisième article a été soumis), jouent un rôle capital pour le pays, car ils donnent des axes d'intervention aux autorités de santé publique laotiennes pour combattre ce fléau. Nous avons choisi une présentation dichotomique des hépatites virales avec une partie dédiée aux hépatites B et une partie dédiée à l'hépatite C en raison de l'émergence d'une troisième question de recherche plus fondamentale. Il s'agit du risque de développement du cancer du foie chez les patients infectés, risque différent suivant que l'on est porteur d'une hépatite B ou d'une C. En effet, les outils diagnostiques pour déterminer la magnitude de ce risque sont peu ou pas accessibles pour les patients au Laos. [...] / The work presented in this thesis reflects many years of effort to devoted the implementation of quality diagnostics of viral hepatitis in Laos at the Centre d'infectiologie Lao Christophe Mérieux (CILM), of which I am now the scientific director. In the introduction, we wanted to present the socio-economic characteristics of Laos and its health challenges in order to achieve the Millennium Development Goals (MDG), which guide public policies in developing countries. We felt it was important to highlight these points as well as the presentation of the CILM to help the readers to understand the context in which this work was carried out. It is thanks to a bio-bank with more than 7,000 biological samples from patients infected with viral hepatitis B and C viruses, that we were able to ask the key research question of this thesis: What is the evolution of patients infected by viral hepatitis B/C who have been follow the viral load at CILM for the past ten years or so? This question raised a second one concerning the quality of diagnosis in health centres in Vientiane. For example, we were interested in the rapid diagnostic kits for hepatitis B used in Vientiane's health facilities, compared to the standard used in our laboratory. This work, which has resulted in the publication of 2 articles (a third article has been submitted), plays a crucial role for the country, as it provides Laotian public health authorities with a focus for action to combat this scourge.

The article deals with a special kind of modelling tass. These problems are used for learning and researching as well. So the results of an empirical study on mathematical modelling of pupils in secondary schools are presented. Pupils of forms 8-10 were observed working on open, realistic problems. These observations were recorded and evaluated. The goal of the presented part of the study is a detailed look at the control processes of modelling problems. In this context changes between real life control and mathematical control during the control phases are studied and evaluated. We describe in detail the sub phases of controlling and explain their connection with modelling process. The problems used in this project can also be used in math lessons, so this kind of research can put teachers and researchers together. These tasks are suitable to support ongoing in-service development and teacher education.

Binge-eating disorder (BED) was first included as its own diagnostic entity in the Fifth Edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) within the Feeding and Eating Disorders section.1 BED’s hallmark feature is recurrent binge eating, involving the consumption of an amount of food that is definitively larger than what others would eat under comparable circumstances within a certain time, associated with a feeling of loss of control over eating. Diagnosis of BED according to DSM-5 (307.59) requires this objective binge eating to occur at least once per week over 3 months. In contrast to binge eating in bulimia nervosa, binge eating in BED occurs without regular inappropriate compensatory behaviors aimed at preventing weight gain, such as self-induced vomiting, fasting, or laxative misuse. Binge eating in BED is further characterized by behavioral abnormalities, such as eating rapidly or until feeling uncomfortably full, and results in marked distress.

The networking of control devices is well advanced in a modern automobile. To ensure secure communications with each other and to prevent mistakes due to communication error, protocol tests are performed at the physical and data link layer level. In a modern car the diagnosis of the electronic devices (e.g. reading/deleting fault memory entries) is done with a standardized communication protocol (UDS - Unified Diagnostic Services). Modern control units have this diagnostic capabilities to detect this communication error among others. This data is provided for a guided troubleshooting the vehicle via a defined interface. In this communication test detection mechanisms are specifically stimulated and read afterwards. In order to read or delete these entries, a diagnosis query is performed on the basis of the diagnostic protocol UDS. The diagnostic requests are currently performed with a diagnostic service of bus analysis software Vector CANoe. This service requests can be performed directly on CAN but for the diagnosis of other bus systems a software gateway is used. The maintenance required and the error rate of the software gateways is very high and should be replaced in the future. Furthermore, additional bus systems can be integrated only with great effort. Therfore the main aim of the master thesis was to develop a concept for univesal UDS API and afterwards implementation of that concept using CAPL programming for modular use in test environments for vehicle communication testing.

Immunological therapy principles are increasingly determining modern medicine. They are used to treat diseases of the immune system, for tumors, but also for infections, neurological diseases, and many others. Most of these therapies base on antibodies, but small molecules, soluble receptors or cells and modified cells are also used. The development of immune checkpoint inhibitors is amazingly fast. T-cell directed antibody therapies against PD-1 or CTLA-4 are already firmly established in the clinic. Further targets are constantly being added and it is becoming increasingly clear that their expression is not only relevant on T cells. Furthermore, we do not yet have any experience with the long-term systemic effects of the treatment. Flow cytometry can be used for diagnosis, monitoring, and detection of side effects. In this review, we focus on checkpoint molecules as target molecules and functional markers of cells of the innate and acquired immune system. However, for most of the interesting and potentially relevant parameters, there are still no test kits suitable for routine use. Here we give an overview of the detection of checkpoint molecules on immune cells in the peripheral blood and show examples of a possible design of antibody panels.

Teoretická část se zabývá typologií některých problémových oblastí u dětí předškolního věku, faktory které ovlivňují jeho osobnost, důležitostí prevence, diagnostiky a nápravy. Praktická část je zaměřena na výzkum vedoucí k analýze nejčastějších problémů a jejich příčin z hlediska učitelek mateřských škol a má za cíl zjistit, jaké osvědčené metody v oblasti prevence, diagnostiky a nápravy pedagogové používají. Klíčová slova dítě, dítě s problémem, rodina, mateřská škola, prevence, pedagogická diagnostika, intervence Theoretical part of my study is aimed to recognize all types of problems of preschool aged children. I have tried to describe areas which might have a possible impact to child's personality regarding the prevention, evaluation and rehabilitation. Practical part of my study explores the most common problems caused by nursery teachers and tries to avoid them in the future by use of proper corrective teaching methods. Key words child, problematic child, family, nursery school, prevention, pedagogical diagnose, feedback

O objetivo desta pesquisa foi avaliar o efeito do manejo do pastejo e do período sobre a concentração dos minerais em diferentes partes da forrageira e sobre o consumo e reciclagem dos minerais por meio do pastejo. O experimento foi realizado de outubro de 1999 a outubro de 2000 em um sistema de pastejo rotacionado em área irrigada de Panicum maximum Jacq. cv. Tanzânia estabelecidas na ESALQ, Piracicaba, SP. O delineamento foi em blocos (4) completos ao acaso com três massas de forragem pós-pastejo (1.000, 25.000 e 4.000 kg/ha de massa seca verde (MSV)), caracterizados, respectivamente, como tratamentos de alta, média e baixa intensidade de pastejo. O período de coleta apresentou diferenças nas concentrações dos minerais em diferentes partes da forrageira. As menores concentrações de nitrogênio e fósforo nas partes analisadas foram observadas no período 01 (30/10/1999 a 14/02/2000), correspondente a primavera e verão. A concentração de potássio variou na maioria das partes analisadas entre os períodos experimentais, sendo os menores valores encontrados no período 02 (15/02/2000 a 01/06/2000). Para o cálcio, ocorreu variação nas concentrações de LFRE (lâmina de folha recém-expandida) e LFM (lâmina de folha madura) entre os períodos ocorrendo maiores valores no período 02. As maiores concentrações de magnésio e enxofre ocorreram no período 03 (02/06/2000 a 23/10/2000). Por outro lado, a intensidade de pastejo demonstrou pouca influência sobre a concentração dos minerais na planta, ocorrendo, na maioria das vezes, apenas diferenças na concentração da LFM, como nos casos do N, P, S e Zn, sendo que estes apresentaram maiores concentrações na LFM no tratamento de alta intensidade. O aumento da intensidade determinou aumento do consumo animal de minerais, com valores de 44,9 kg N; 4,0 kg P; 54,2 kg K; 6,1 kg Ca; 8,3 kg Mg e 2,6 kg S por hectare, por ciclo de pastejo, no tratamento de alta intensidade. Do mesmo modo, maiores quantidade e porcentagem de minerais contidos na forragem pré-pastejo retornaram ao solo via fezes e urina quando a pastagem foi manejada com alta intensidade. Assim, as diferenças entre consumo e reciclagem de minerais de acordo com a intensidade de pastejo podem determinar diferenças nas adubações. A partir das determinações desse primeiro ano experimental, e das coletas de forragem do segundo ano experimental, entre fevereiro de 2002 e janeiro de 2003, em áreas irrigadas e de sequeiro estabelecidas com capim Tanzânia, foi determinada a norma DRIS para o capim Tanzânia. O método DRIS, com base nas normas determinadas a partir de uma população de alta produção, demonstrou-se acurado para diagnosticar as amostras de tecido foliar como deficientes, equilibradas e excessivas. No entanto, existiu pouca relação entre IBN (índice de balanço nutricional) e produção de massa seca. Quanto aos métodos utilizados para cálculo das funções, o proposto por Jones (1981) demonstrou menores variações entre os índices DRIS e não apresentou relação do IBN com a produção de massa seca, o que determinou a indicação dos métodos propostos por Beaufils (1973) e Elwali & Gascho (1984) para o cálculo do índice DRIS. / The objective of this research was to evaluate the effects of grazing management and season of the year on mineral concentrations in four parts of Panicum maximum Jacq. cv. Tanzânia (EL - expanded leaf, RELB - recent expanded leaf blades, MLB - mature leaf blades and SS - stems plus sheaths), and on mineral intake and recycling through grazing cattle. One experiment was done from October 1999 to October 2000 on an irrigated area under rotational grazing. Treatments imposed were three post-grazing residues, 1000, 2500 and 4000 Kg/ha of green dry matter, respectively in a four randomized complete blocks design. Season of the year affected mineral concentrations in every part of the plant. From October till beginning of February (period 1) there was low concentration of nitrogen and phosphorous in all parts of the plant. Potassium concentrations varied among season of the year; its lowest concentrations occurred from February to May (period 2). Calcium concentration of the RELB and MLB, also varied along seasons of the year occurring highest values in period 2. The highest concentrations of magnesium and sulphur occurred for June to October (period 3). Grazing intensity had small influence on plant mineral concentrations. The MLB showed higher concentrations of N, P, S and Zn on the low post-grazing residue. Mineral intake was higher on the low post-grazing residue; intake levels during one grazing cycle at the low post-grazing residue were 44.9 kg N; 4.0 kg P; 54.2 kg K; 6.1 kg Ca; 8.3 kg Mg e 2.6 kg S per hectare. A higher mineral percentage of the pre-grazing forage mass was returned to the soil via feces and urine when the pasture was managed with low post-grazing residue. Differences between intake and mineral recycling related to the grazing intensity can be used to establish the adequate fertilization for pasture maintenance and production. The DRIS norms for the Tanzania grass were determined using data colleted during two periods: October 1999 through October 2000 and February 2002 through January 2003. The DRIS method, based on the established norms, was accurate to diagnose deficiencies, balance and excess nutrients in leaf tissue. There was a low relationship between NBI (Nutritional Balance Index) and dry matter yield. The method proposed by Jones (1981) showed smaller variations between DRIS indexes and showed no relationship between NBI and dry matter yield. Methods proposed by Beaufils (1973) and Elwali & Gascho (1984) were more suitable to estimate DRIS index.

A variação na disponibilidade de nitrogênio e magnésio pode alterar as características produtivas, nutricionais e metabólicas das plantas forrageiras. O objetivo foi de avaliar o efeito das combinações de doses de nitrogênio e de magnésio na produção da parte aérea e das raízes, nas concentrações dos nutrientes nas lâminas de folhas recém-expandidas (LR) e nas raízes, na atividade da glutamina sintetase, nas concentrações das formas inorgânicas de nitrogênio e na relação nitrogênio:magnésio nas LR do capim-marandu. O experimento foi realizado em casa de vegetação, em Piracicaba SP, com a Brachiaria brizantha cv. Marandu. Foram estudadas cinco doses de nitrogênio (2; 9; 16; 23 e 30 mmol L-1) e cinco doses de magnésio (0,05; 0,70; 1,35; 2,00 e 2,65 mmol L-1) em solução nutritiva, em esquema fatorial 52 fracionado, perfazendo treze combinações: 2/0,05; 2/1,35; 2/2,65; 9/0,70; 9/2,00; 16/0,05; 16/1,35; 16/2,65; 23/0,70; 23/2,00; 30/0,05; 30/1,35 e 30/2,65. O delineamento experimental foi o de blocos ao acaso, com quatro repetições. As plantas tiveram dois períodos de crescimento, nos quais foram avaliadas as produções de massa seca, contados perfilhos e folhas, coletado material fresco para determinação da atividade da glutamina sintetase, determinada a área foliar e determinada as concentrações dos nutrientes e nitrato e amônio nas lâminas das folhas recém-expandidas. Após o segundo corte as raízes foram separadas da parte aérea sendo determinada a massa seca das raízes, o comprimento e superfície radiculares e as concentrações dos nutrientes nas raízes. As combinações de altas doses de nitrogênio e magnésio resultaram em alta produção de massa seca, número de folhas e perfilhos, área foliar, massa seca das raízes, e comprimento e superfície radicular do capim-marandu. As concentrações de nitrogênio (segundo corte) e cálcio (nos dois cortes) nas LR e a concentração de nitrogênio nas raízes aumentaram com as combinações de elevadas doses de nitrogênio e de magnésio. A concentração de potássio nas LR, nos dois cortes, e nas raízes da gramínea foi mais baixa quando se utilizaram combinações de elevadas doses de nitrogênio e magnésio. De forma isolada, o incremento nas doses de nitrogênio resultou em reduzidos comprimento e superfície específicos das raízes, mais alta concentração de nitrogênio nas LR e mais alta concentração de cálcio nas raízes e o incremento das doses de magnésio proporcionou pequenos comprimento e superfície específicos das raízes, mais alta concentração de magnésio nas LR e nas raízes e mais baixa concentração de cálcio nas raízes do capim. A concentração de nitrato nas folhas diagnósticas foi influenciada pelas combinações de doses de nitrogênio e magnésio enquanto a concentração de amônio aumentou com o incremento das doses de nitrogênio. A atividade da glutamina sintetase foi mais baixa em altas doses de nitrogênio, nos dois crescimentos da gramínea. A relação de 6,3:1 entre as concentrações de nitrogênio e de magnésio nas LR correspondeu à máxima produção de massa seca no primeiro corte do capim. / Productive, nutritional and metabolic characteristics of the forage plants may be changed by the nitrogen and magnesium availability. The objective was to study the effects of nitrogen and magnesium rates on marandu palisadegrass growth, by evaluating the shoot and root yield, leaf and root concentrations of nutrients and leaf concentration of inorganic forms of nitrogen, glutamine synthetase activity and nitrogen:magnesium ratio in recently expanded leaf laminae (RL). The experiment was carried out in a greenhouse at Piracicaba, State of São Paulo, Brazil, with the Brachiaria brizantha cv. Marandu. Five nitrogen rates (2, 9, 16, 23 and 30 mmol L-1) and five magnesium rates (0.05, 0.70, 1.35, 2.00 and 2.65 mmol L-1) were used in nutrient solutions, in a fractorial 52 factorial, resulting in the following combinations: 2/0.05, 2/1. 35, 2/2.65, 9/0.70, 9/2.00, 16/0.05, 16/1.35, 16/2.65, 23/0.70, 23/2.00, 30/0.05, 30/1.35 and 30/2.65. A randomized block design, with four replications was used. Plants had a growth and regrowth period. After the second harvest it was evaluated the dry mass yield, number of tillers and leaves, sampled fresh material to assay the glutamine synthetase activity, measured the leaf area, and determined the concentrations of nutrients, nitrate and ammonium in the recently expanded leaf laminae (diagnostic leaves). After the second harvest the roots were separated from the tops and was determined the root dry mass, total length and surface, specific length and surface, and concentrations of nutrients in such plant tissue. High nitrogen and magnesium rates resulted in high dry mass yield, leaf area, number of leaves and tillers, in both growth periods of the grass, as well as in roots dry mass, length and surface. Nitrogen (at the second harvest) and calcium (both harvests) concentrations in diagnostic leaves and nitrogen concentration in the roots was high when high rates of nitrogen nitrogen were combined with high rates of magnesium. Diagnostic leaves, in the two grown periods, and roots sampled had low potassium concentrations in plants growth with high rates of both nitrogen and magnesium. Increases in nitrogen rates increased nitrogen concentration in the diagnostic leaves and calcium concentration in the roots, and decreased root specific length and surface. Increases in magnesium rates increased magnesium concentration in the diagnostic leaves and roots, but decreased root calcium concentration and root specific length and surface. Ammonium concentration was increased by nitrogen rates and nitrate concentration was higher under magnesium deficiency. Glutamine synthetase, determined at the two growth periods, decreased with increasing nitrogen rates. Maximum dry mass yield in the first harvest of the grass was found when the nitrogen and magnesium concentrations ratio was 6.3:1 in the diagnostic leaves.

O cultivar Piatã de Brachiaria brizantha é uma gramínea forrageira de lançamento recente para uso nas pastagens brasileiras e a pesquisa relativa à nutrição mineral deste capim é escassa. O nitrogênio é o principal responsável pelo aumento da produtividade em plantas forrageiras, porém a associação com o enxofre pode influir nas respostas das plantas forrageiras à adubação. Mediante a combinação de doses de nitrogênio com doses de enxofre, objetivou-se estudar as características morfogênicas, nutricionais e produtivas do capim-piatã cultivado em solução nutritiva, empregando-se a sílica como substrato. O experimento foi realizado em casa de vegetação e estudou cinco doses de nitrogênio (2; 9; 16; 23 e 30 mmol L-1) e cinco doses de enxofre (0,1; 0,7; 1,3; 1,9 e 2,5 mmol L-1), em esquema fatorial 52 fracionado, em delineamento de blocos casualizados, com quatro repetições. As plantas foram submetidas a dois cortes, sendo o primeiro aos 44 dias após o transplantio e o segundo 25 dias após o primeiro corte. A interação doses de nitrogênio x doses de enxofre foi significativa para o número total de folhas e perfilhos, taxa de aparecimento de folhas e filocrono, área foliar, massa seca da parte aérea e das raízes, valor SPAD, comprimento e superfície radiculares totais, concentração de nitrogênio e de enxofre nos tecidos foliares e radiculares, concentração de nitrato e relação nitrogênio total:enxofre total (Nt:St) em pelo menos um dos dois ciclos de crescimento do capim-piatã. As mais elevadas doses de nitrogênio e de enxofre maximizaram o crescimento e desenvolvimento da parte aérea e do sistema radicular das plantas. Doses elevadas de nitrogênio diminuíram o comprimento radicular específico e a relação enxofre-sulfato:enxofre total (S-SO4:St) nas folhas diagnósticas do capim, além de aumentar a atividade da enzima redutase do nitrato e a concentração de amônio nesses tecidos. A dose de enxofre de aproximadamente 1,3 mmol L-1 proporcionou o maior comprimento e superfície radiculares específicos, enquanto a dose mais alta de enxofre (2,5 mmol L-1) maximizou a concentração de enxofre-sulfato e a relação S-SO4:St nas folhas diagnósticas das plantas. Correlações significativas e positivas foram constatadas entre a concentração de nitrogênio total, valor SPAD e a concentração de enxofre total das folhas diagnósticas e a produção de massa seca das plantas, nos dois cortes do capim-piatã. A concentração de nitrogênio nas folhas diagnósticas do capim-piatã e o valor SPAD servem como bons parâmetros para a avaliação do estado nutricional das plantas em relação ao nitrogênio, enquanto para a avaliação do estado nutricional do enxofre nas folhas diagnósticas do capim-piatã, a concentração de enxofre total e a relação Nt:St devem ser tomadas em conjunto. / Piatã cultivar of Brachiaria brizantha is a recently released forage grass to be used in brazilian pastures and research on mineral nutrition of this grass is scarce. Nitrogen is the main nutrient to increase forage productivity, but the association with sulphur can influence plant response to fertilization. Through the combination of nitrogen rates with sulphur rates, the objective was to study the morphological, nutritional and productive characteristics of Piatã palissadegrass grown in nutrient solution, by using ground quartz as substrate. The experiment was conducted in greenhouse and five rates of nitrogen (2, 9, 16, 23 and 30 mmol L-1) were combined with five rates of sulphur (0.1, 0.7, 1.3, 1.9 and 2.5 mmol L-1) in a 52 fractionated factorial set in randomized block design, with four replications. Plants were harvested two times, the first harvest was done 44 days after transplanting and the second one was 25 days after the first harvest. The interaction nitrogen rates x sulphur rates was significant for the total number of leaves and tillers, leaf appearance rate, phyllochron, leaf area, dry mass of shoots and roots, SPAD value, root length and root surface, nitrogen and sulphur concentrations in the diagnostic leaves and roots, nitrate concentration and total nitrogen:total sulphur ratio in at least one of the two growth cycles of Piatã palissadegrass. High nitrogen and sulphur rates maximized growth of shoots and roots of the plants. High nitrogen rates resulted in low specific root length and sulfate-sulphur:total sulphur ratio (SSO4: St) in diagnostic leaves, in addition to increasing the activity of the nitrate reductase and ammonium concentration in diagnostic tissue. Sulphur rate about 1.3 mmol L-1 provided the highest specific root length and surface, while the highest sulphur rates (2.5 mmol L-1) maximized sulfate-sulphur concentration and S-SO4:St ratio in diagnostic leaves. There was a significant and positive correlation between the concentration of total nitrogen, SPAD value and total sulphur concentration in diagnostic leaves with plant dry matter production, in both harvests of Piatã palissadegrass. Nitrogen concentration and SPAD value in diagnostic leaves of Piatã palissadegrass can be used as good parameters for assessing the nutritional status of plants in relation to nitrogen, while for the assessment of sulphur nutrition status in diagnostic leaves of Piatã palissadegrass, the concentration of total sulphur and total nitrogen:total sulphur ratio should be taken together.

INTRODUÇÃO: A dor pélvica crônica não visceral constitui desafio clínico, justificável pela diversidade de estruturas presentes na pelve e pelo amplo arcabouço musculoesquelético que a envolve, sustenta e protege, o que dificulta seu diagnóstico e tratamento. Várias são as causas de sua ocorrência e vários sistemas orgânicos podem estar nela envolvidos, isolada ou associadamente, incluindo-se especialmente o geniturinário, o gastrointestinal, o neuropsicológico e o musculoesquelético. OBJETIVOS: Caracterizar-se clínica e demograficamente uma amostra composta de mulheres com dor pélvica crônica de origem não visceral refratária ao tratamento convencional e avaliar-se do efeito analgésico da estimulação magnética transcraniana repetitiva (EMTr) na magnitude da dor, psiquismo, função sexual e qualidade de vida. CASUÍSTICA E MÉTODOS: Dezoito doentes foram aleatorizadamente incluídas em dois grupos (A e B) de estudo para receber tratamento inicial com EMTr ativa (EMTr-a) ou EMTr sham (EMTr-s) respectivamente, aplicadas na área de representação da pelve e períneo do córtex motor primário. EMTr-a foi realizada com a frequência de 10Hz; 80% do limiar motor de repouso totalizando 3000 pulsos por sessão. A EMTr-s foi realizada com uma bobina desconectada e uma segunda bobina ativa aplicada perpendicularmente à primeira e ligada de modo a emitir sons e reverberações sobre o escalpo semelhantes ao da EMTr. Três semanas após a sessão de EMTr-a, as doentes do grupo A foram tratadas com EMTr-s e as do grupo B com EMTr-a. As avaliações foram realizadas no momento basal (D-7), e nos dias, -1, +7, + 21, +28 e +36 do início do estudo. Foram utilizados os seguintes inventários validados para a língua portuguesa: Inventário Breve de Dor; DN-4, Questionário de descritores breve de dor McGill, Inventário de Sintomas de Dor Neuropática, Escala Hospitalar de Ansiedade e Depressão, Índice da Função Sexual Feminina, WHOQOL-breve, Escala Visual Analógica e Questionário para Avaliação da Dor Pélvica e um algiometro para avaliar a sensibilidade dolorosa muscular na pelve e quadril. RESULTADOS E CONCLUSÕES: A DPC era intensa, havia escores elevados de ansiedade e depressão, a dor causou impacto negativo nas atividades físicas e diárias, na autopercepção do estado de saúde, na função sexual feminina e na qualidade de vida e houve elevada ocorrência da síndrome dolorosa miofascial nos músculos pélvicos e do quadril. A EMTr-a proporcionou melhora significativa da dor nas doentes tratadas inicialmente com EMTr-a e a EMTr-s causou melhora significativa e menos marcante da dor quando foi precedida do tratamento com a EMTr-a. Não houve modificação significativa dos valores dos escores de depressão e ansiedade e dos valores dos escores do Índice da Função Sexual Feminina nas doentes inicialmente tratadas com EMTr-a e ocorreu aumento do valor do escore ansiedade nas doentes inicialmente tratadas com EMTr-s. Houve aumento dos valores dos domínios \"psicológico\" e \"meio ambiente\" do WHOQOL-breve nas doentes tratadas inicialmente com EMTra. O resultado do tratamento inicial com EMTr-a ou EMTr-s influenciou o resultado do segundo procedimento. O limiar motor em repouso estava elevado, a inibição intracortical reduzida e a facilitação intracortical normal. Houve correlação entre o limiar motor de repouso e o maior número de descritores afetivos do Questionário de Dor McGill e entre a redução da inibição intracortical e o número aumentado de descritores afetivos do mesmo questionário. A estimulação magnética transcraniana repetitiva é procedimento seguro e alternativa terapêutica para doentes com DPC / INTRODUCTION: Non-visceral chronic pelvic pain is clinical challenge due to the diversity of structures present in the pelvis and the widespread musculoskeletal framework that surrounds, supports and protects, which makes its diagnosis and treatment very difficult and usually unsatisfactory. Many are its causes and various organs may be involved in its occurrence, either alone or in association, especially the genitourinary, gastrointestinal, musculoskeletal and neuropsychological structures. OBJECTIVES: Clinical and demographic characterization of a sample of women with non-visceral chronic pelvic pain refractory to conventional treatments and evaluation of the analgesic efficacy of repetitive transcranial magnetic stimulation (rTMS) in the magnitude of pain, psychic, sexual function and quality of life. PATIENTS AND METHODS: Eighteen patients were randomly included into two groups (A and B) accordingly the use of active rTMS (rTMS-a) or sham rTMS (rTMS-s), respectively, applied in the representation area of the pelvis and motor cortex perineum primary as the first approach. rTMS-a was performed with 10Hz, 80% of the muscle resting threshold and 3000 pulses per session. s-rTMS was performed with a disconnected coil and a second coil applied perpendicularly to the first one to generate sounds and reverberations on the scalp similar to rTMS-a. Three weeks after the session of rTMS-a the group A patients were treated with rTMS-s and the group B patients with rTMS-a. The evaluations were performed at baseline (D-7), and on days -1, +7, +21, +28 and +36 from the start of the study. The following inventories validated for the Brazilian-Portuguese language were used, Brief Pain Inventory were used; DN-4, Questionnaire brief descriptors of McGill Symptoms Inventory Neuropathic Pain, Hospital Anxiety and Depression Scale, Female Sexual Function Index, WHOQOL-brief, Visual Analogue Scale and the Questionnaire for Assessment of Pelvic Pain. A muscle algometer to assess muscle soreness in the pelvis and hip was also used. RESULTS AND CONCLUSIONS: The DPC was severe, the patients presented high scores of anxiety and depression, pain caused negative impact on physical and daily activities, self-perceived health status, female sexual function and quality of life and there was high incidence of myofascial pain syndrome the pelvic muscles and hip. rTMS-a provided significant improvement of pain in patients initially treated with rTMS-a and rTMS-s resulted in significant but less expressive improvement in pain when it was preceded by treatment with rTMS-a. There was no significant change in the values of the scores of depression and anxiety and of the Female Sexual Function Index in patients initially treated with rTMS-a it and there was an increase in the value of the score anxiety in patients initially treated with rTMS-s. There was improvement of the \"psychological\" and \"environment\" dominions of the WHOQOL-brief in patients initially treated with rTMS-a. The results of initial treatment with rTMS-a or rTMS-s influenced the outcome of the second procedure. The resting motor threshold was high, the intracortical inhibition was reduced, and the intracortical facilitation normal. There was correlation between the resting motor threshold and the affective descriptors of the McGill Pain Questionnaire and between reduced intracortical inhibition and the increased number of affective descriptors of the same questionnaire. rTMS is a safe procedure and therapeutic alternative for patients with DPC