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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Novel aspects of the metabolism & toxicology of the chemotherapeutic agent, Irinotecan (CPT-11)

Dodds, Helen Maree Unknown Date (has links)
No description available.
2

Organism Migration in Soils: Should We Be So Comfortable With Diagnosing Ancient Infectious Diseases?

Lawler, Dennis F., Tangredi, Basil P., Widga, Christopher C. 01 May 2020 (has links)
Studies of the ancient history of infectious diseases have been facilitated greatly by development of a succession of novel analytical methods. In particular, laboratory analytical methods that are based on high-throughput ancient deoxyribonucleic acid sequencing have received considerable attention in this respect. Even so, significant environmental caveats remain. There are many means by which microbes move through soil, often fairly readily. Thus, the depositional component of the postmortem environment, especially with respect to unshielded animal or human remains, is a fertile arena for many microbes that can contaminate archaeological specimens well after deposition and decay of soft tissues. The huge number of pathogenic and nonpathologic genera and species clearly dictate renewed interest and research into the long-term biological activities of soil-covered remains. In a tuberculosis context, we focus on various depositional concerns and limitations, such as contamination prior to archaeological discovery (perhaps many years prior), various means of microorganism movement in soil, the influence of these factors on differential diagnosis, and real hazards for misinterpretation of investigational results.
3

Review of diagnostic methods in the most cited articles for anorexia nervosa, bulimia nervosa, and binge-eating disorder

Roberts, Rebecca Chapman 09 August 2008 (has links)
Diagnostic practices utilized in studies of participants with Anorexia Nervosa, Bulimia Nervosa, and Binge-Eating Disorder were investigated. A reliable coding system was used by two reviewers to analyze the diagnostic documentation practices in articles from the top-thirty most cited articles for each diagnostic category. Interrater agreements were all above .95. Results showed that many important diagnostic practices and criteria are either not being employed or not being documented. Uniform reporting procedures are necessary to help readers know how each article’s sampling procedure and subject pool differs from other samples used in the literature. Researchers reported the most details about sample characteristics with the recently proposed Binge-Eating Disorder category. Discussion focuses on identifying the specific diagnostic and sampling procedures deserving better documentation in the eating disorder literatures.
4

Diagnostické metody plošného rozložení defektů solárních článků / Diagnostic Method Used to a Location of Solar Cells Defects

Jandová, Kristýna January 2009 (has links)
This doctoral thesis deals with analysis of existing area defect detection methods in solar cells and with concept of its innovation and of the development of faster detection method. Results of measurement is analyzing in practical and theoretical part. The most important is LBIC (Light Beam Induced Current) method innovated of different wavelength light source usage and Electroluminescence method. On the bases of this knowledge is created Fast LBIC method and then is created catalog of defects in monocrystalline silicon solar cells.
5

Prevalência da mastite bovina: estudo comparativo de métodos para sua detecção e do comportamento de vários agentes etiológicos frente à prova da redução do azul de metileno / Prevalence of bovine mastitis: a comparative study of methods for its detection and the behavior of several etiological agents against the test of reduction of methylene blue

Nader Filho, Antonio 22 August 1983 (has links)
Foram submetidas a prova do \"California Mastitis Test\"(CMT), 468 vacas leiteiras pertencentes a seis propiedades rurais produtoras de leite tipo B, localizadas na região do Município de Barretos, SP. Das 56 (12,0 por cento ) fêmeas reagentes a esta prova, 48 (1O,3 por cento ) foram confirmadas através do isolamento e identificação do agente etiológico. A elevada concordância (85,71 por cento ) verificada entre, a prova do CMT e o exame bacteriológico, confirma a eficiência desta prova na detecção de transtôrnos da glândula mamária não perceptíveis clínicamente, uma vez que nenhuma das fêmeas analisadas apresentou sinais da enfermidade. A contagem leucocitária e a pesquisa do teor de cloretos efetuadas nas 56 fêmeas reagentes a prova do CMT, apresentaram concordâncias de 87,5 por cento e 50,0 por cento , respectivamente, em relação ao exame bacteriológico. O Staphylococcus aureus foi o agente etiológico mais frequente (52,08 por cento ) nos casos de mastite bovina. O estudo comparativo entre a contagem padrão em placas e a prova da redução do azul de metileno, efetuado nas 56 amostras reagentes a prova do CMT, revelou que 30 (53,57 por cento ) amostras apresentaram tempo de redução não condizente com o número de microrganismos determinado, isto é, tempo de redução menor que 3 l/2 horas e contagem total de microrganismos inferior a 500.000 microrg/ml. Observou-se, ainda, que destas 30 amostras, 26 (86,67 por cento ) apreesentaram contagens leucocitárias superiores a 2.500.000 leucócitos/ml., sugerindo deste modo, uma provável interferência dos leucócitos quando em número elevado, na prova da redução do azul de metileno. O estudo comparativo entre a contagem padrão em placas e a prova da redução do azul de metileno, efetuado nas 30 amostras negativas a prova do CMT, revelou que 27 (90,0 por cento ) amostras apresentaram o tempo de redução condizente com o número de microrganismos determinado, o que confirma a importância de sua utilização na rotina do Serviço de Inspeção. A provãvel interferência dos leucócitos não compromete a eficiência desta prova, pois as variações observadas ocorrem no sentido de abreviar o tempo de redução, sendo muito pequena a probabilidade de aceitação de um produto com o número de microrganismos superior ao permitido pelo Ministério da Agricultura. Dentre as provas utilizadas pelo Serviço de Inspeção na detecção da mastite bovina a nível de Estabelecimentos Industriais a prova de Whiteside foi a que apresentou maior sensibilidade e especificidade, capaz de revelar a presença de leite anormal em rebanhos cuja prevalência desta doença foi igual ou superior a 7,0 por cento das fêmeas ou 1,4 por cento dos quartos analisados. A pesquisa do teor de cloretos apresentou menor sensibilidade e especificidade, incapaz de revelar a presença de leite anormal em todos os rebanhos analisados. Entretanto, este fato não invalida a utilidade de sua aplicação, pois esta prova pode ser de fundamental importância na detecção de fraudes no leite. A prevalência da mastite bovina em até 20,0 por cento das fêmeas leiteiras ou em ate 5,0 por cento dos quartos examinados, não foi suficiente para colocar os quesitos analisados nas amostras de leite, fora dos padrões normais. Acredita-se que estes padrões devam ser objeto de estudos posteriores. / Four-hundred and sixty-eigth milk cows, belonging to six type B milk producing dayry farms, located in the region of Barretos, SP, Brazil were submitted to \"California Mastitis Test\", of the fifty-six (12,0 per cent ) cows positive to the mentioned test, forty eigth (10,31) had the diagnosis confirmed throght isolation and identification of the etiologic agent. High agreement (85,7 per cent ) was obtained between CMT and bacteriologic examination, which confirms the efficiency of CMT in the detection of diseases of the mammary glands, not perceptible by clinical examination, as none the animals examined presented signs of disease. Leucocyte count and determination of chloride level performed on the fifty-six reactors presented agreement of 87,5 per cent and 50,0 per cent , respectively, with bacteriological examination. Staphylococcus aureus was the most frequently isolated bacterium (52,08 per cent of cases). Comparison between total plate count and reductase test, performed on thirty samples negative to CMT, showed that twenty-seven samples (90,0 per cent ) presented a reduction time compatible with the number of microorganisms present, confirming the importance of reductase test regarding inspection. The theoretical interference of leucocytes was not important in practice, as variations observed were abreviation of reduction time, rendering the propability of accepting a product with a number of microorganisms over legal limits very small. Considering tests used by the Inspection Service for detection of bovine mastitis, the Whiteside test presented highest sensivity and specificity, as it was capable of reveling abnormal milk when prevalence was 7,0 per cent and over, considering animals and 1,1 per cent considering quarters. Chloride level presented lower sensitivity and specificity as it was unable to detect abnormal milk in all dairy farms. However it is very important in the detection of frauds. Prevalence of mastitis of up to 20,0 per cent of animals or of 5,0 per cent of quarters was not sufficient to bring the total milk produced below standards.
6

Métodos de diagnóstico em modelos autoregressivos simétricos / Diagnostic Methods in Symmetric Autoregressive Models

Medeiros, Marcio Jose de 17 November 2006 (has links)
Os modelos autoregressivos simétricos são modelos de regressão em que os erros são correlacionados -- AR(1) -- e pertencem à classe de distribuições simétricas. O objetivo deste trabalho é discutir métodos de diagnóstico de influência para esses modelos. Para ilustrar a metodologia, são apresentados exemplos do modelo de precificação de ativos (CAPM). / The symmetric autoregressive models are regression models in which the errors are correlated and belong to the class of symmetrical distributions. The aim of this work is to discuss influence diagnostic methods for those models. To illustrate the methodology, examples of Capital Asset Pricing Models (CAPM) are presented.
7

Presença de Leishmania sp em equinos de zona urbana de Uruguaiana, Rio Grande do Sul

Escobar, Taiane Acunha 16 April 2015 (has links)
Submitted by Marcos Anselmo (marcos.anselmo@unipampa.edu.br) on 2016-09-12T14:39:20Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) TAIANE ACUNHA ESCOBAR.pdf: 973361 bytes, checksum: 098a27b7377e80f12957e7c2e3378ef3 (MD5) / Approved for entry into archive by Marcos Anselmo (marcos.anselmo@unipampa.edu.br) on 2016-09-12T14:39:36Z (GMT) No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) TAIANE ACUNHA ESCOBAR.pdf: 973361 bytes, checksum: 098a27b7377e80f12957e7c2e3378ef3 (MD5) / Made available in DSpace on 2016-09-12T14:39:36Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) TAIANE ACUNHA ESCOBAR.pdf: 973361 bytes, checksum: 098a27b7377e80f12957e7c2e3378ef3 (MD5) Previous issue date: 2015-04-16 / A Leishmaniose é uma doença parasitária infeciosa, causada por protozoários do gênero Leishmania e transmitida pelo vetor flebótomo. Caracterizada como Doença Tropical Negligenciada, acomete diversas espécies de mamíferos, sendo o cão, atualmente, o principal reservatório em área urbanas. Os equinos também podem ser infectados, especialmente quando estão em contato com reservatórios ou vetores. No município de Uruguaiana – RS há um expressivo número de equinos utilizados na tração de cargas e como meio de transporte, com constante movimentação dentro do perímetro urbano. Esses animais vivem em condições precárias, submetidos a trabalhos excessivos e má nutrição. Frente a estes fatores somados à atual situação epidemiológica da leishmaniose visceral canina no município, o presente estudo foi realizado com o objetivo de identificar a presença de Leishmania sp em equinos urbanos do município de Uruguaiana-RS. Para a condução do experimento foram utilizadas amostras sanguíneas de 192 equinos testadas em três técnicas: sorológica (ELISA), imunocromatográfica (TR-DPP) e molecular (PCR). Na técnica de ELISA foi utilizado soro, testado com o Kit Ensaio Imunoenzimático para Diagnóstico da Leishmaniose Visceral Canina – Bio-Manguinhos e para o teste imunocromatográfico Teste Rápido Dual Path Platform utilizaram-se amostras de sangue total. As reações de PCR, após extração de DNA de sangue periférico dos animais, foram realizadas com quatro pares de iniciadores distintos. Todas as amostras testadas apresentaram-se não reagentes nos ensaios imunológicos. Entretanto, com o emprego da técnica PCR, mais sensível, houveram amostras positivas. Dos quatro pares de iniciadores testados, 75 amostras foram positivas, 52 com pelo menos um dos pares. Contudo, ao analisarmos individualmente os iniciadores, 58,6% foram positivos para LITSV/L5.8SR, 44% para LITSV/LISTSR, 28% para RV1/RV2, e 4% LITSR/L5.8S. Os resultados apresentados no experimento indicam a possibilidade de existência de Leishmania em equinos na região de Uruguaiana, embora os testes sorológicos não tenham apresentado reatividade para Leishmania. A técnica molecular possibilitou a detecção do gênero Leishmania nas amostras de sangue periférico dos equinos. Este foi o primeiro relato da infecção na espécie equina na região do extremo oeste do estado do Rio Grande do Sul. Contudo, se faz importante a realização de sequenciamento do fragmento para que se possa confirmar a identidade genética de Leishmania sp. / Leishmaniasis is an parasitic infectious disease caused by protozoa genus Leishmania and transmitted by the sandfly vector. Characterized as Neglected Tropical Disease, affects several species of mammals, and the dog are main reservoir in urban areas. The horses can also be infected especially when they are in contact with reservoirs or vectors. In Uruguaiana’s city, there is a significant number of horses used in the tensile loads and means of transport, with constant movement within the city, living in precarious work’s conditions, subjected to excessive and poor nutrition. In view of these factors added to the current epidemiological situation of LVC in the city, the present study aim to identify the presence of Leishmania in urban horses the municipality of Uruguaiana-RS. For the experiment, blood samples from 192 horses were used for holding three techniques: serological (ELISA), immunochromatographic (TR-DPP) and molecular (PCR). For conducting ELISA’s Test serum was used and tested with Bio-Manguinhos kit and for TR-Dual Path Platform Rapid Test whole blood samples were employed. In the PCR technique, DNA was extracted from peripheral blood of animals and amplifications were performed with primers RV1 / RV2, LITSR / LITSV, LITSR / L5.8S / LITSV / L5.8SR.. All tested samples showed negative results in immunoassays. However, employing sensitive techniques such as PCR, positive samples were detected. Considering the four primer pairs tested, 75 animals were positive, 52 with at least one of the pairs. However, when analyzing the individual primers, 58.6% were positive for LITSV/L5.8SR, 44% to LITSV/LISTSR, 28% for RV1/RV2, and 4% LITSR/L5.8S.In The results presented in the experiment indicate the possibility of Leishmania in horses in the region of Uruguaiana city, although serological tests have not submitted reactivity to Leishmania. Molecular technich shows results to consider the Leishmania’s presence in horse’s peripheral blood samples. This was the first report of infection in equine species in westernmost region of the Rio Grande do Sul state. However, it is important to conduct sequencing of the fragment so that we can confirm the genetic identity of Leishmania sp.
8

Streptococcus pneumoniae : epidemiological, clinical and serological studies

Burman, Lars Å. January 1993 (has links)
A retrospective study of invasive pneumococcal disease in patients from Greater Göteborg in 1964- 1980 identified 125 cases of meningitis, 305 of pneumonia, 61 of septicemia with unknown focus, and 17 with other manifestations, all verified by cultures from normally sterile body fluids. The incidence was several times higher in infants and in the elderly than in any other age-group. A wide variety of underlying conditions were present in 23% of the infants, 34% of the children, and 81% of the adults. In adults alcoholism was known in one third of the cases. The case fatality rate was 24% among patients with underlying conditions and 9% among previously healthy individuals. The case fatality rate was 50% in patients with hospital-acquired infection. Twohundred-fifteen pneumococcal strains, isolated from blood or CSF from 1971 to 1983 at the laboratories of clinical bacteriology of Göteborg, Malmö, and Umeå were serotyped by coagglutination (COA). Of all isolates, 89% belonged to serotypes represented in the 23-valent vaccine. In a separate study COA was compared with counterimmunoelectrophoresis (CIE). COA was found to have several advantages; rapidity, lower cost, and ability to disclose serotypes with neutral charge, which constituted 19% of all strains. In a prospective study the etiology was determined in 196 hospitalized patients with pneumonia, most of them community-acquired. Culture of specimens from blood, transtracheal aspirate (TTA), sputum, and nasopharynx, assays of antigen in sputum, urine, and TTA, and assays of pneumococcal antibodies to capsular polysaccharide, C-polysaccharide, and pneumolysin in paired sera were performed. The etiology was established in 64% of the patients. Streptococcus pneumoniae was the most common agent (32%). In a serological study of patients with pneumococcal infection, diagnosed by culture of CSF, TTA, or blood, IgG antibodies against C-polysaccharide and pneumolysin were determined by ELISA. The diagnostic sensitivity was only 51% and 60%, respectively. In conclusion, invasive pneumococcal disease is strongly overrepresented at tender and high age and in patients with concomitant conditions, notably alcoholism. S. pneumoniae remains a predominant causative agent of community-acquired pneumonia in adults needing hospitalization. Due to the low sensitivity and/or specificity of individual microbiological techniques, a combined use of several techniques is necessary when trying to assess the relative importance of pneumococci and other agents in pneumonia. Extended use of the currently available pneumococcal vaccine and development of improved pneumococcal vaccines seem highly warranted. / <p>Diss. (sammanfattning) Umeå : Umeå universitet, 1993, härtill 5 uppsatser.</p> / digitalisering@umu.se
9

Avaliação de lesões e agentes bacterianos causadores de pericardite em suínos / Assessment of injuries and bacterial agents that cause pericarditis in pigs

Coelho, Carolini Fraga January 2011 (has links)
O presente trabalho avaliou corações com pericardite e pulmões correspondentes através da análise anatomopatológica, bacteriológica, histopatológica e da técnica da Reação em Cadeia da Polimerase (PCR) a fim de identificar os agentes envolvidos no processo e correlacioná-los com as lesões macroscópicas presentes. O estudo foi realizado em quatro frigoríficos do estado do Rio Grande do Sul com o apoio do Serviço de Inspeção Federal (SIF) durante o período de fevereiro a outubro de 2010. Foram coletadas 120 amostras de corações com pericardite e pulmões correspondentes e avaliadas 3.487 carcaças na linha de inspeção em 20 lotes diferentes, totalizando 8 coletas. Os materiais foram examinados nos laboratórios de Microbiologia, Biologia Molecular e Patologia Veterinária da Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul. Entre as pericardites, 86,7% foram classificadas como fibrinosas (104/120), 6,7% serosas (8/120), 2,5% (3/120) sero-fibrinosas e 4,2% (5/120) amostras não puderam ser classificadas. Foram diagnosticadas lesões de endocardite em somente 2,5% (3/120) dos corações. Quanto aos pulmões analisados, as alterações macroscópicas mais encontradas foram 66,6% (80/120) aderências de pleura direita e/ou esquerda, 19,1% (23/120) hepatizações dos lobos apicais direito e 5,83% (7/120) do esquerdo, 23,33% (28/120) do lobo cardíaco direito e 23,33% (28/120) do esquerdo. Pela análise histopatológica do coração, a lesão mais frequente foi presença de tecido de granulação denso fibroso e em algumas amostras tecidos de granulação frouxo, com proliferação de vasos em ambos os casos. Havia presença de fibrina e células inflamatórias mononucleares em alguns casos. Na análise dos pulmões correspondentes, 70% (84/120) das amostras de pulmões apresentavam algum tipo de lesão aparente. As lesões mais comuns foram pleurite crônica, hiperplasia das células do tecido linfóide associado aos brônquios (BALT), presença de macrófagos alveolares e broncopneumonia purulenta. Não foram observadas lesões microscópicas em 23,3% (28/120) dos pulmões e 6,6% (8/120) não foram coletados para análise devido a cronicidade da lesão. A análise da carcaça mostrou pleurite parietal em 12,5% (15/120) e algum tipo de envolvimento articular em 1,6 % (2/120) dos animais, não sendo objetivo avaliar o tipo de lesão e sim uma possível correlação com Haemophilus parasuis. Das análises bacteriológicas dos corações, obteve-se um maior número de isolados de Pasteurella multocida 16% (20/120), seguido de Streptococcus spp. 17,3% (6 /120) e 5% (6/120) Haemophilus parasuis. A maior parte dos isolados foi obtida das pericardites fibrinosas. Com relação aos pulmões correspondentes, foram isolados 17,5% (22/120) Pasteurella multocida, 51.57% (8/120) Streptococcus spp., e 0,8% (1/120) Haemophilus parasuis. Entre os isolados de P. multocida foi realizada a tipificação capsular das cepas identificadas no pulmão e coração através da PCR e todas pertenciam ao tipo A. Na técnica da PCR no coração pode-se observar um grande número de M. hyopneumoniae 34,8% (39/112), seguido de A. pleuropneumoniae 23,2% (26/112), H. parasuis 6,2% (7/112), P. multocida tipo A 3,5% (4 /112) e M. hyorhinis 2,6% (3/112). A mesma técnica aplicada aos pulmões correspondentes, revelou M. hyopneumoniae 81,2% (91 /112), H. parasuis 36,6 % (41 /112), A. pleuropneumoniae 33,9% (38/112), P. multocida tipo A 30,3% (34/112), M. hyorhinis 9.8% (11/112). / The present work assessed hearts with pericarditis and the corresponding lungs through visual analysis of gross lesions, bacteriology, histopathology and polymerase chain reaction (PCR) to identify agents involved in the processes and to correlate them with the lesions. The study was carried out in four slaughterhouses from the state of Rio Grande do Sul, Brazil, with the help of the Service of Federal Inspection (SIF) during the period of February to October, 2010. Samples of 120 hearts with pericarditis and corresponding lungs were collected and examined, and 3.487 carcasses were assessed in the slaughter line in 20 different batches, in a total of 8 sample collections. Materials were examined in the laboratories of Microbiology, Molecular Biology and Pathology of the Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul, Brazil. Among the samples with pericarditis, 104 were classified as fibrinous (86.7%), 8 serous (6.7%), 3 sero-fibrinous (2.5%) and 5 remained unclassified (4.2%). Lesions of endocarditis were present in 3 hearts (2.5%). Regarding lungs, the commonest gross changes were 80 pleural adhesions (66.6%) and 23 consolidations of the right apical lobes of the lung (19.1%) and 7 (5,83%) of the left; 28 (23,33%) from right cardiac lobe and 28 (23,33%) of left. Using histopathological analysis of the heart, the most frequent lesions were the presence of dense fibrous granulomatous tissue and, in some samples, loose granulation tissue, with of vessels proliferation in both cases. It was also found fibrin and inflammatory mononuclear cells in some cases. In the analysis of the corresponding lungs, 84 (70%) of the samples showed some type of apparent lesion. Te most prevalent lesions were chronic pleuritis, hyperplasia of lymphoid tissue cells associated to bronchia (BALT), presence of lung macrophages and purulent bronchopneumonia. Twenty eight lungs (23.3%) did not show any microscopic lesion and 8 (6.6%) were not collected because of the chronicity of the lesion. The analysis of the carcass showed parietal pleurisy in 15 (12.5 %) and joint involvement in 2 (1.6 %), but the present study did not intend to assess such lesions but a possible correlation with Haemophilus parasuis infection. Regarding bacteriological examination of the heart, Pasteurella multocida was the biggest isolation rate 16% (20/120), followed by Streptococcus spp 17.3% (6 /120) and 5% (6/120) Haemophilus parasuis. Most isolates were obtained from fibrinous pericarditis. Regarding the corresponding lungs, Pasteurella multocida was isolated in 17.5% (22/120) materials, Streptococcus spp in 51.57% (8/120), and Haemophilus parasuis in 0.8% (1/120). Pasteurella multocida capsular typing was carried out from strains identified through PCR from heart and lungs, all belonged to the serotype A. By PCR from samples from pericarditis a large number of M. hyopneumoniae was detected: 34. 8% (39/112), followed by APP 23.2% (26/112), H. parasuis 6.2% (7/112), P. multocida type A 3.5% (4 /112) and M. hyorhinis 2.6% (3/112). The same technique applied to the corresponding lungs, showed M. hyopneumoniae in 81.2% (91 /112), H. parasuis in 36.6 % (41 /112), APP in 33.9% (38/112), P. multocida type A in 30.3% (34/112) and M. hyorhinis in 9.8% (11/112).
10

Avaliação de lesões e agentes bacterianos causadores de pericardite em suínos / Assessment of injuries and bacterial agents that cause pericarditis in pigs

Coelho, Carolini Fraga January 2011 (has links)
O presente trabalho avaliou corações com pericardite e pulmões correspondentes através da análise anatomopatológica, bacteriológica, histopatológica e da técnica da Reação em Cadeia da Polimerase (PCR) a fim de identificar os agentes envolvidos no processo e correlacioná-los com as lesões macroscópicas presentes. O estudo foi realizado em quatro frigoríficos do estado do Rio Grande do Sul com o apoio do Serviço de Inspeção Federal (SIF) durante o período de fevereiro a outubro de 2010. Foram coletadas 120 amostras de corações com pericardite e pulmões correspondentes e avaliadas 3.487 carcaças na linha de inspeção em 20 lotes diferentes, totalizando 8 coletas. Os materiais foram examinados nos laboratórios de Microbiologia, Biologia Molecular e Patologia Veterinária da Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul. Entre as pericardites, 86,7% foram classificadas como fibrinosas (104/120), 6,7% serosas (8/120), 2,5% (3/120) sero-fibrinosas e 4,2% (5/120) amostras não puderam ser classificadas. Foram diagnosticadas lesões de endocardite em somente 2,5% (3/120) dos corações. Quanto aos pulmões analisados, as alterações macroscópicas mais encontradas foram 66,6% (80/120) aderências de pleura direita e/ou esquerda, 19,1% (23/120) hepatizações dos lobos apicais direito e 5,83% (7/120) do esquerdo, 23,33% (28/120) do lobo cardíaco direito e 23,33% (28/120) do esquerdo. Pela análise histopatológica do coração, a lesão mais frequente foi presença de tecido de granulação denso fibroso e em algumas amostras tecidos de granulação frouxo, com proliferação de vasos em ambos os casos. Havia presença de fibrina e células inflamatórias mononucleares em alguns casos. Na análise dos pulmões correspondentes, 70% (84/120) das amostras de pulmões apresentavam algum tipo de lesão aparente. As lesões mais comuns foram pleurite crônica, hiperplasia das células do tecido linfóide associado aos brônquios (BALT), presença de macrófagos alveolares e broncopneumonia purulenta. Não foram observadas lesões microscópicas em 23,3% (28/120) dos pulmões e 6,6% (8/120) não foram coletados para análise devido a cronicidade da lesão. A análise da carcaça mostrou pleurite parietal em 12,5% (15/120) e algum tipo de envolvimento articular em 1,6 % (2/120) dos animais, não sendo objetivo avaliar o tipo de lesão e sim uma possível correlação com Haemophilus parasuis. Das análises bacteriológicas dos corações, obteve-se um maior número de isolados de Pasteurella multocida 16% (20/120), seguido de Streptococcus spp. 17,3% (6 /120) e 5% (6/120) Haemophilus parasuis. A maior parte dos isolados foi obtida das pericardites fibrinosas. Com relação aos pulmões correspondentes, foram isolados 17,5% (22/120) Pasteurella multocida, 51.57% (8/120) Streptococcus spp., e 0,8% (1/120) Haemophilus parasuis. Entre os isolados de P. multocida foi realizada a tipificação capsular das cepas identificadas no pulmão e coração através da PCR e todas pertenciam ao tipo A. Na técnica da PCR no coração pode-se observar um grande número de M. hyopneumoniae 34,8% (39/112), seguido de A. pleuropneumoniae 23,2% (26/112), H. parasuis 6,2% (7/112), P. multocida tipo A 3,5% (4 /112) e M. hyorhinis 2,6% (3/112). A mesma técnica aplicada aos pulmões correspondentes, revelou M. hyopneumoniae 81,2% (91 /112), H. parasuis 36,6 % (41 /112), A. pleuropneumoniae 33,9% (38/112), P. multocida tipo A 30,3% (34/112), M. hyorhinis 9.8% (11/112). / The present work assessed hearts with pericarditis and the corresponding lungs through visual analysis of gross lesions, bacteriology, histopathology and polymerase chain reaction (PCR) to identify agents involved in the processes and to correlate them with the lesions. The study was carried out in four slaughterhouses from the state of Rio Grande do Sul, Brazil, with the help of the Service of Federal Inspection (SIF) during the period of February to October, 2010. Samples of 120 hearts with pericarditis and corresponding lungs were collected and examined, and 3.487 carcasses were assessed in the slaughter line in 20 different batches, in a total of 8 sample collections. Materials were examined in the laboratories of Microbiology, Molecular Biology and Pathology of the Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul, Brazil. Among the samples with pericarditis, 104 were classified as fibrinous (86.7%), 8 serous (6.7%), 3 sero-fibrinous (2.5%) and 5 remained unclassified (4.2%). Lesions of endocarditis were present in 3 hearts (2.5%). Regarding lungs, the commonest gross changes were 80 pleural adhesions (66.6%) and 23 consolidations of the right apical lobes of the lung (19.1%) and 7 (5,83%) of the left; 28 (23,33%) from right cardiac lobe and 28 (23,33%) of left. Using histopathological analysis of the heart, the most frequent lesions were the presence of dense fibrous granulomatous tissue and, in some samples, loose granulation tissue, with of vessels proliferation in both cases. It was also found fibrin and inflammatory mononuclear cells in some cases. In the analysis of the corresponding lungs, 84 (70%) of the samples showed some type of apparent lesion. Te most prevalent lesions were chronic pleuritis, hyperplasia of lymphoid tissue cells associated to bronchia (BALT), presence of lung macrophages and purulent bronchopneumonia. Twenty eight lungs (23.3%) did not show any microscopic lesion and 8 (6.6%) were not collected because of the chronicity of the lesion. The analysis of the carcass showed parietal pleurisy in 15 (12.5 %) and joint involvement in 2 (1.6 %), but the present study did not intend to assess such lesions but a possible correlation with Haemophilus parasuis infection. Regarding bacteriological examination of the heart, Pasteurella multocida was the biggest isolation rate 16% (20/120), followed by Streptococcus spp 17.3% (6 /120) and 5% (6/120) Haemophilus parasuis. Most isolates were obtained from fibrinous pericarditis. Regarding the corresponding lungs, Pasteurella multocida was isolated in 17.5% (22/120) materials, Streptococcus spp in 51.57% (8/120), and Haemophilus parasuis in 0.8% (1/120). Pasteurella multocida capsular typing was carried out from strains identified through PCR from heart and lungs, all belonged to the serotype A. By PCR from samples from pericarditis a large number of M. hyopneumoniae was detected: 34. 8% (39/112), followed by APP 23.2% (26/112), H. parasuis 6.2% (7/112), P. multocida type A 3.5% (4 /112) and M. hyorhinis 2.6% (3/112). The same technique applied to the corresponding lungs, showed M. hyopneumoniae in 81.2% (91 /112), H. parasuis in 36.6 % (41 /112), APP in 33.9% (38/112), P. multocida type A in 30.3% (34/112) and M. hyorhinis in 9.8% (11/112).

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