Šio baigiamojo darbo tikslas – įvertinti imunochromatografinio tyrimo metodo patikimumą, atliekant Žmogaus imunodeficito viruso aspektų analizę. Siekiant įvertinti imunochromatografinio tyrimo metodo patikimumą, ŽIV tyrimai buvo atliekami atrankiniu FIRST RESPONSE HIV CARD TEST 1-2.0 testu, pagrįstu nagrinėjamu metodu. Tyrimas atliktas Infekcinių ligų ir tuberkuliozės ligoninės, VšĮ Vilniaus universiteto ligoninės Santariškių klinikų filialo laboratorijoje. ŽIV tyrimai atlikti visiems 18 - 60 metų amžiaus pacientams, hospitalizuotiems į Infekcinių ligų ir tuberkuliozės ligoninės Žvėryno stacionarą, taip pat ambulatoriškai konsultuojamiems dėl nepatikslintos kilmės karščiavimo, bėrimo, limfadenopatijos, mononukleozinio sindromo, užsitęsusio arba besikartojančio viduriavimo bei virusinių hepatitų nuo 2013-01-01 iki 2013-12-31. Gauti teigiami mėginiai buvo siunčiami į Nacionalinę visuomenės sveikatos priežiūros laboratoriją, kurioje atlikti patvirtinamieji tyrimai dėl ŽIV. Ne visi teigiami mėginiai buvo patvirtinti, tačiau šio tyrimo metu buvo atskleista 14 naujų ŽIV atvejų per metus. Siekiant nustatyti gautų klaidingai teigiamų rezultatų susidarymo priežastį, buvo atrinkti pacientai, kuriems nebuvo patvirtinti atrankiniai ŽIV tyrimai. Išnagrinėjus pagrindines jų tuometines diagnozes, nustatyta kad daugiausiai klaidingai teigiamų rezultatų gaunama sergant šiomis infekcinėmis ligomis: rože, Epštein – Baro sukelta mononukleoze ir hepatitu A. / The main goal of this paper is to assess the reliability of the immunochromatographic method carrying out the analysis of human immunodeficiency virus aspects. In order to assess the reliability of immunochromatographic method, the HIV tests were performed using the selective FIRST RESPONSE CARD TEST HIV 1-2.0 test, based on the method in question. The assay was carried out in the Laboratory of National Tuberculosis and Infectious disease hospital in Vilnius. The HIV tests were carried out from 01-01-2013 till 31-12-2013 to all patients aged 18-60 years, who were hospitalized in the National Tuberculosis and Infectious disease hospital in Vilnius as well as to outpatient, who were consulted due to fever of unspecified origin, rash, lymphadenopathy, mononucleosis syndrome, prolonged or recurrent diarrhea and viral hepatitis. All positive samples were sent to National Public Health Surveillance Laboratory for final confirmation. Not all the samples were confirmed, but this assay revealed 14 new cases of HIV per year. In order to identify the reasons of false positive results, the patients, whose selective HIV tests were not approved, have been selected and after the examination of their diagnoses, it was found that the majority of false-positive results were received to patients with the following infections: rose, mononucleosis induced by Epstein - Barr virus and hepatitis A.

The aim of this diploma thesis was determination of tractor engines oil drain interval. In theoretical chapters is described theoretical background of the field of engines lubrication and issue of use of motor oils in practice. The drain intervals were determined using a tribotechnical diagnosis of collected samples of a motor oil from tractors. The analysis of the samples were processed in a laboratory and the dominant parameters that influence the wear of the oil were detected. On the basis of the application of a trend analysis were determined the reference values of the levels of oil degradation. After taking the evaluation and discussion of the results were defined oil drain intervals for the tractors. At the end there was made an evaluation of the results and operational recommendations. The thesis includes also an economical evaluation.

The main goal of this thesis is focused on comparing the selected properties of mineral and synthetic oils. For this was used operating monitoring and laboratory experiment and with them was compared properties. At operation was observed oxidation, sulfation, nitration, TAN, TBN and the content of metal elements in the oil. In Laboratory was experimental investigated the effect of increased temperature and change of color , viscosity and lubricity. The theoretical part of the thesis describes the basic of tribology and lubrication engineering, types of lubricants and oil properties and their production and distribution.

The aim of this thesis was to carry out a search based on available literature that focused on historical development of timbered houses in the Czech Republic that are intended for permanent living. A survey was conducted subsequently to this search. This survey concentrated on firms that nowadays build timbered houses and also on construction solutions and structure composition of such houses. The conducted survey describes prevalent construction solutions of timbered houses in the current housebuilding sector of the Czech market. The thesis focuses on thermal properties of today's timbered wooden structures. Next, an indicative measurement of these buildings was carried out by a blower door test and thermal imaging diagnostics. Furthermore, the thesis compares timbered structures with standard requirements for permanent housing. Interestingly, the measurements for instance showed that one new building met the requirements of the blower door test. Next, several thermography measurements demonstrated seamless heat transfer of a timbered wall as a whole. The conclusion of the thesis summarizes the current use and construction of timbered houses in the Czech Republic. Moreover, it sums up the results of the measurements and outlines the survey results regarding the thermal properties of these houses.

Nosemosis is a serious disease of bees caused by microsporidia Nosema apis and Nosema ceranae. Both species are widely spread around the world and in the Czech Republic. The aim of this thesis was to evaluate the incidence and prevalence of Nosema spp., describe the species variability and assess the influence of the season. PCR method amplifying part of the gene encoding the small ribosomal subunit rRNA was used to identify the species of Nosema spp. A total 77 samples originated from 17 farmers were examined Out of them, 71% (55 samples) were positive for the presence of Nosema spp. Samples were collected in five seasons in 2011?2012. Both N. apis, and N. ceranae were detected in all breeds. In 2011, N. apis was detected as causative agent of nosemosis except one sample, where the mixed infection was detected. In 2012, N. ceranae was observed in mono- or mixed infections. Currently monoinfections of N. apis were not detected in 2012. Generally, the highest occurrence was detected in the autumn and winter months.

Nosematosis belongs to the intestinal disease of bees, which is widely spread both in the world and in the Czech Republic. It is caused by Nosema apis and Nosema ceranae. The aim of this diploma thesis was to evaluate the occurrence of the Nosema dispute in the territory of ČSV Bystřice, to describe species variability and to assess the effect of the season on the occurrence of Nosema apis and Nosema ceranae. To detect infection of colonies, 5 bees from selected hives were examined under the microscope. In positive specimens on nosematosis, the species of Nosema spore was molecularly examined. In total, 93 samples from 15 breeders were examined. Samples were taken in the spring and summer of 2017. The sampling took place both for malachists and for farmers with more than 150 bee colonies. During the summer sampling more positive bees were found than in spring sampling. Beekeepers were further assessed by the number of hives. Following the analysis of the samples obtained using the PCR method, Nosema ceranae disputes were present in the vast majority of colonies.

No description available.

The DSM-5 Alternative Personality Disorder Model includes Personality Inventory for DSM-5 (PID-5) that assess 25 specific personality traits in 5 domains. The aim of this thesis was to evaluate the method and its clinical benefits, especially by comparing it with the IPDE structured interview method on a sample of N = 29 psychiatric patients. Quantitative analysis of the results of both methods revealed a number of statistically significant correlations between the IPDE dimensional scores and the individual PID-5 traits of corresponding disorders. For disorders equally defined by both approach, the IPDE dimensional scores and PID-5 individual traits correlated by majority. Due to the overall low prevalence of undisputed personality disorder diagnosis in the sample, a qualitative analysis of 11 selected cases was performed. This analysis revealed the overlapping of the personality traits derived from both methods in 10 of these 11 cases. The methods differ in the assignment of a specific personality disorder as a diagnosis, but this is due in particular to a different theoretical definition of the methods. Overall, the PID-5 method has been evaluated as clinically beneficial, with a promising future and wide use in clinical practice. KEYWORDS Personality disorders, diagnostics, PID-5, IPDE

This diploma work raises the issues of preconcepts for didactics of students of teaching, deals with the perception of those concepts by primary school teachers, and subsequently with their diagnosis . The aim of this work is to find out to what extent the perception of some chosen didactic concepts are connected comparing students of pedagogical faculty and experienced primary school teachers. The theoretical part aims to define the concepts involved in the research and concerns with constructivists didactics. In the practical part the data relating to preconcepts were found and processed by method of semantic differential.

The samples under investigation were collected in Department of Pediatric Infectious Diseases of the University Hospital (Brno). Group of patients (100) was heterogeneous in terms of symptoms, age and sex. The samples were taken from patients with an LB diagnosis and from those with nonspecific symptoms. Molecular typing of LB spirochetes in clinical samples (104 blood/serum, 89 cerebro-spinal fluid and 1 synovial fluid) became necessary when the general immunological tests gave unclear results. The samples were analyzed using PCR-based and molecular biology techniques that include: nested- and spacer-PCR, specie-specific PCR, sequence, virtual hybridization, in silico RFLP analysis, similarity search. Results of conducted analysis confirmed that 51% of samples (98) were positive on B. bugrdorferi sensu lato. Using above mentioned techniques 6 spirochete species from B. burgdorferi sensu lato complex were identified; two of them weren?t detected in samples of human origin in Europe yet. Comparative analysis of two media for Borrelia cultivation from samples of human origin definitely proved the adventage of using MKP instead of traditionally used BSK-H Complete.