M.Sc. (Botany) / Please refer to full text to view abstract

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim of this study was to analyze the development of cravina culture in substrate ash rice husk in different vessel sizes and under varying levels of water availability, observing its response as the height of the stems developed, number of nodes per stem; fresh weight and dry weight of stems and number of flower buds developed per stem. The cultivar used in the experiment was a hybrid of series 'Melody', the seedlings were obtained by cutting a die makers deployed specifically for this purpose. The seedlings were first planted in rooting where they remained for 30 days and were then transplanted in black plastic pots, 8 and 18 liters, filled with a substrate of rice husk ash, and kept 100% 80% 60% and 40% of the capacity of water retention of the vessels. Maintenance of the water vessel was performed by the method of weighting, with added amounts of water to keep the vessel in your weight limit. The water consumption of plants was determined by the method of weighing over two crop cycles. Results showed that the water consumption of this species is highly influenced by the weather; is recommend the use vessel of lower capacity and maintenance water in 40% of the capacity to retain water; it is preferable to growing the cravina in just a cycle, since the rods obtained in the second exhibit features not fit for commercialization. / O objetivo do presente trabalho foi analisar o desenvolvimento da cultura da cravina em substrato de cinza de casca de arroz em diferentes tamanhos de vaso e sob variados níveis de disponibilidade hídrica, observando sua resposta quanto à altura das hastes desenvolvidas; número de nós por haste; peso fresco e peso seco das hastes e número de botões florais desenvolvidos por haste. A cultivar utilizada no experimento foi o híbrido interespecífico série Melody , sendo que as mudas foram obtidas por meio de estaquia de um matrizeiro implantado especificamente para tal objetivo. As mudas obtidas primeiramente foram plantadas em bandejas de enraizamento onde permaneceram por 30 dias, sendo após transplantadas em vasos plásticos pretos de 8 e 18 litros, preenchidos com substrato de cinza de casca de arroz, e mantidas em 100%, 80%, 60% e 40% do limite da capacidade de retenção hídrica dos vasos. A manutenção da umidade dos vasos foi realizada a partir do método de pesagens, sendo acrescentadas quantidades variáveis de água para manter o vaso no peso estipulado. O consumo de água das plantas também foi determinado através do método de pesagens, ao longo de dois ciclos de cultivo. Os resultados obtidos mostraram que o consumo hídrico dessa espécie é altamente influenciado pelas condições meteorológicas; recomenda-se o uso do vaso de menor capacidade e a manutenção da disponibilidade hídrica em 40% da capacidade de retenção de água; é preferível o cultivo da cravina em apenas um ciclo, já que as hastes obtidas no segundo apresentam características não próprias para a comercialização.

Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2018-05-24T13:16:05Z No. of bitstreams: 1 texto completo.pdf: 1295210 bytes, checksum: 4ce6920b272a35167656916056179c03 (MD5) / Made available in DSpace on 2018-05-24T13:16:05Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1295210 bytes, checksum: 4ce6920b272a35167656916056179c03 (MD5) Previous issue date: 2018-02-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Plantas medicinais dominam a farmacologia por proverem várias fontes de remédios e assim, a mesma espécie de planta pode ter compostos que melhoram e compostos que prejudicam a espermatogênese. Dianthus caryophyllus apresenta ação antibiótica, antifúngica, antiviral, antioxidativa, sedativa e tranquilizante. Teve ação no controle da pressão arterial em ratos e possível efeito antiurolítico, porém nenhum estudo foi feito para avaliar seus efeitos na reprodução. Assim, esse estudo visou avaliar o efeito do extrato aquoso da flor de Dianthus caryophyllus (EADC) nos testículos de camundongos Balb/c, além da resposta de enzimas antioxidante à planta. Quarenta e oito animais foram distribuídos aleatoriamente em seis grupos experimentais (n=8): G1 (Controle), G2 (Viagra), G3, G4, G5 receberam diariamente 10, 20, 40 mg/kg de EADC, respectivamente, e o G6 recebeu EADC 40mg/kg em dias alternados. Foram realizadas análises morfométricas e estereológicas do parênquima testicular, avaliação de alterações estruturais do tecido, atividade das enzimas catalase (CAT), superóxido dismutase (SOD) e glutationa S-transferase (GST), além da concentração de malondialdeido (MDA) e de óxido nítrico (ON) nos testículos. Houve redução do diâmetro, proporção, volume e área do lúmem tubular, aumento da proporção e área de epitélio seminífero em todos os grupos tratados com a planta, sendo que o diâmetro tubular reduziu apenas em G5. Houve redução da proporção de túbulos seminíferos normais e aumento de vacúolos em todos os grupos. No intertúbulo houve aumento de tecido conjuntivo em G5. A proporção de células de Leydig aumentou apenas em G6, assim como o volume das células de Leydig por grama de testículo no mesmo grupo. O número de células de Leydig por testículo aumentou apenas em G6, enquanto por grama de testículo houve aumento em G5 e G6. Quanto à volumetria dos componentes, houve aumento do volume de tecido conjuntivo em G4, G5 e G6. Houve aumento de CAT em G5 e G6 e de MDA em todos os grupos tratados. Pode-se inferir que houve aumento da produção espermática em todos os grupos, no intertúbulo nota-se mais efeitos em Leydig e tecido conjuntivo principalmente nos grupos G5 e G6 e que a planta gera estresse oxidativo. / Medicinal plants dominate pharmacology by providing various sources of medicines and thus, the same plant species may have components that improve and impair spermatogenesis. Dianthus caryophyllus has antibiotic, antifungal, antiviral, antioxidative, sedative and tranquilizing actions. It had action in controlling blood pressure in rats and possible antiurolithic effects, but no study was done to evaluate its effects on reproduction. The aim of this study was evaluate the effect of the aqueous extract of the Dianthus caryophyllus flowers (AEDC) on the testis of Balb/c mice and the antioxidant enzyme response to the plant. 48 animals were randomly distributed in six experimental groups (n = 8): G1 (Control), G2 (Viagra), G3, G4, G5 received daily 10, 20, 40 mg / kg AEDC respectively and G6 40 G / AEDC 40mg / kg every other day. Morphometric and stereological analyzes of the testicular parenchyma, evaluation of tissue structural alterations, activity of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST), and malondialdehyde (MDA) and oxide nitric oxide (ON) in the testicles. There was a reduction in the diameter, proportion, volume and area of the tubular lumen, increase in the proportion and area of seminiferous epithelium in all groups treated with the plant, and reduction of tubular diameter only in G5. There was a reduction in the proportion of normal seminiferous tubules and increase of vacuoles in all groups. In the intertubular compartment there was reduction in the proportion of lymphatic space as well as increase of connective tissue in all the treated groups. The proportion of Leydig cells increased only in G6, as did the volume of Leydig cells per gram of testis in the same group. The number of Leydig cells per testis increased only in G5, while per gram of testis there was increase in G5 and G6. In the volumetry of the components, there was an increase of only connective tissue in G4, G5 and G6. There was an increase in CAT in G5 and G6 and in MDA in all treated groups. It can be concluded that there was an increase in sperm production in all groups, in the intertubular compartment there were more effects in Leydig and connective tissue, especially in the G5 and G6 groups, and that the plant generates oxidative stress.

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Soil contamination is an environmental, social and economic problem. In wine areas, prolonged use of phytosanitary products, the copper base, contributed to the accumulation of this element in the soil, when in excess is highly damaging to the development of plants, impacting on their productive potential. In order to minimize the harmful effect of Cu in soils of these areas, this study aimed to evaluate the cultivation and the potential extraction of this element in three floricultural species: calla lily (Zantedeschia spp.), dianthus (Dianthus chinensis L.) and chrysanthemum (Dendranthema grandiflora Tzevelev), to play the role phytoremediation in soil, seeking a low environmental impact agriculture. Three separate experiments were carried out from October 2013 to December 2014, in a protected environment in the Floriculture sector in UFSM, Santa Maria, RS. The soil was collected in areas of viticulture. And, the contents of Cu added were in the ground by means of copper sulphate. In Chapter I evaluated the development and the water consumption of calla lily under different irrigation strategies and copper levels in the soil. The trial was in DIC (completely randomized design), with factorial arrangement 4x3 (four copper levels and three irrigation strategies), with five repetitions. It was found that these levels of added Cu in soil does not interfere with the daily water consumption calla lily, obtaining average daily 0.5, 0.8 and 1.2 mm day-1 for the blades 40, 60 and 80% CRA (water retention capacity of the vessel), respectively, and that this species is tolerant crop in this environment. In Chapter II evaluated the development and the water consumption of dianthus and its effect on the culture in ground presenting excess Cu. The test was DIC in factorial 4x3 (four copper levels and three irrigation strategies), with five repetitions. It was found that Cu content added to the soil will not interfere with the daily water consumption dianthus obtaining daily averages 0.42; 0.72 and 0.91 mm day-1 for the blades 40, 60 and 80% CRA, respectively, and the results showed fitorremediativa fitness grapevine species to soils with excess of this metal. Chapter III evaluated the development of chrysanthemum cv. Dark Fiji grown in soil with excess Cu. The test was DIC in factorial 5x2 (five levels of added Cu in soil and two crop cycles), with five repetitions. It was found that the increase of Cu in the soil affect the normal development and growth of the culture, however, it showed tolerance to crop species in soil with excess Cu, accumulating high concentrations of this element in the roots. We conclude so, overall, the three species have tolerance culture in ground presenting excess Cu, highlighting the dianthus as promising phytoremediation soil. / A contaminação do solo é um problema ambiental, social e econômico. Em áreas vitivinícolas, como as da Serra Gaúcha, o uso prolongado de produtos fitossanitários, a base de cobre, contribuiu para o acúmulo deste elemento no solo, quando em excesso é altamente prejudicial ao desenvolvimento das plantas, impactando no seu potencial produtivo. Visando minimizar o efeito nocivo do Cu nos solos destas áreas, o presente trabalho objetivou avaliar o cultivo e o potencial de extração deste elemento em três espécies florícolas: calla lily (Zantedeschia spp.), cravina de jardim (Dianthus chinensis L.) e crisântemo (Dendranthema grandiflora Tzevelev), para desempenhar o papel fitorremediador no solo, buscando uma agricultura de baixo impacto ambiental. Foram realizados três distintos experimentos, no período de outubro de 2013 a dezembro de 2014, em ambiente protegido no Setor de Floricultura da UFSM, Santa Maria, RS. O solo utilizado foi coletado em áreas de vitivinicultura, na Serra Gaúcha. E, os teores adicionados de Cu no solo foram por meio de sulfato de cobre. No capítulo I avaliou-se o desenvolvimento e o consumo hídrico da calla lily submetida a diferentes estratégias de irrigação e teores de cobre no solo. O ensaio foi em DIC (delineamento inteiramente casualizado), com esquema fatorial 4x3 (quatro teores de cobre e três estratégias de irrigação), com cinco repetições. Verificou-se que estes teores do Cu adicionado no solo não interferiram no consumo hídrico diário da calla lily, obtendo médias diárias de 0,5, 0,8 e 1,2 mm dia-1 para as lâminas de 40, 60 e 80% CRA (capacidade de retenção de água no vaso), respectivamente e, que esta espécie apresenta tolerância de cultivo neste ambiente. No capítulo II avaliou-se o desenvolvimento e o consumo hídrico da cravina de jardim e seus efeitos sobre o cultivo em solo apresentando excesso de Cu. O ensaio foi em DIC, em esquema fatorial 4x3 (quatro teores de cobre e três estratégias de irrigação), com cinco repetições. Verificou-se que os teores do Cu adicionados ao solo não interferiram no consumo hídrico diário da cravina de jardim, obtendo médias diárias de 0,42; 0,72 e 0,91 mm dia-1 para as lâminas de 40, 60 e 80% CRA, respectivamente e, os resultados demonstraram aptidão fitorremediativa da espécie para solos vitivinícolas com excesso deste metal. No capítulo III avaliou-se o desenvolvimento do crisântemo cv. Dark Fiji cultivado em solo com excesso de Cu. O ensaio foi em DIC, em esquema fatorial 5x2 (cinco teores adicionados de Cu no solo e dois ciclos de cultivo), com cinco repetições. Verificou-se que o incremento de Cu no solo afetou o crescimento e desenvolvimento típico da cultura, entretanto, a espécie apresentou tolerância ao cultivo em solo com excesso de Cu, acumulando altas concentrações deste elemento nas raízes. Conclui-se de maneira, geral, que as três espécies apresentam tolerância ao cultivo em solo apresentando excesso de Cu, destacando a cravina de jardim como promissora a fitorremediação do solo.

Höga kvävenedfall och förändringar inom jord- och skogsbruk har lett till igenväxning av svenska sandmarker. Sand- och grustäkter kan fungera som tillflyktsort för kärlväxtarter som är beroende av de torra och näringsfattiga markförhållanden som finns i sandmarker. Vapnö grustäkt i Hallands län undersöktes 2010 och visade sig ha en hög biologisk mångfald med bland annat 145 kärlväxtarter, varav fyra rödlistade. Syftet med den här studien var att se hur artsammansättningen av kärlväxter ser ut idag, sju år senare. Området inventerades vid tio tillfällen från april till augusti och samtliga kärlväxtarter noterades. Resultatet blev en lista på 224 arter varav 63 var återfynd från 2010 och 161 var nya arter för området. Det fanns fem rödlistade arter: Knippnejlika (Dianthus armeria) (EN), hårginst (Genista pilosa) (NT), backtimjan (Thymus serpyllum) (NT), skogsalm (Ulmus glabra) (CR) och naverlönn (Acer campestre) (CR). Det totala antalet arter och rödlistade arter har ökat sen 2010, vilket tyder på en positiv utveckling av områdets biologiska mångfald. Den höga artmångfalden beror troligen på det öppna, varierande landskapet och de rödlistade arterna växer där tack vare den alltmer ovanliga sandmarken. Områdets mångfald av kärlväxter hotas av igenväxning samt konkurrens från de invasiva arterna blomsterlupin (Lupinus polyphyllus) och parkslide (Fallopia japonica). Detta kan motverkas av störningar i form av slåtter, uppgrävning, naturvårdsbränning eller bete. / Nitrogen deposition and changes in agriculture and forestry have led to a decrease of sandy habitats in Sweden due to regrowth of vegetation. Sand- and gravelpits can act as refuges for vascular plants that require the dry and nutrient-poor conditions of sandy soils. Vapnö gravelpit in Halland county in Sweden was surveyed in 2010 and proved to hold a high biodiversity including 145 species of vascular plants, of which four were red-listed. Now, seven years later, the purpose of this study was to see how the composition of vascular plants has changed since then. The area was studied on ten occasions from April to August, and all species of vascular plants were noted. The study resulted in a list of 224 species, of which 63 were remaining since 2010 and 161 were new species for the area. There were five red-listed species: Dianthus armeria (EN), Genista pilosa (NT), Thymus serpyllum (NT), Ulmus glabra (CR) and Acer campestre (CR). The total number of species and red-listed species have increased, suggesting a positive development of the biodiversity of the area. The high species diversity is probably due to the open, varied landscape and the presence of the red-listed species due to the increasingly rare sandy habitat. The diversity of vascular plants in the area is threatened by regrowth of vegetation, as well as the invasive species Lupinus polyphyllus and Fallopia japonica. These threats can be counteracted by disturbance in the form of mowing or digging up plants, prescribed burning or grazing.

Abstract This MSc. thesis deals with karyological, genetic and phenotypic variation of selected taxa from the genus Dianthus L. in the Czech Republic and adjacent countries. The evolutionary history of the genus has been shaped by several microevolutionary processes, including interspecific hybridization, genome duplication, and edaphic speciation. These processes led to the origin of a number of phenotypically similar taxa, which are often restricted to a narrow geographic area. One subendemic and three endemic taxa occur in the Czech Republic. These (sub)species were used as model groups to gain insight into microevolutionary processes in small populations and the postglacial development of the genus in Central Europe. The thesis consists of three parts, each addressing different evolutionary phenomenon: Dianthus arenarius subsp. bohemicus is a critically endangered endemic psammophyte currently known from a single population in Central Bohemia. The site is also inhabited by widespread D. carthusianorum. Interspecific hybridization has been suspected on the basis of morphological characters, but this has never been confirmed by any other technique. I exploited differences in the number of chromosomes between both species and, with the aid of DAPI flow cytometry, estimated relative DNA contents of...

This diploma's thesis deals with critically endangered species Dianthus arenarius subsp. bohemicus from family Caryophyllaceae. Its distribution range is now limited on its last natural locality in National natural monument Kleneč. As a result of the change in land use (especially the abandonment of grazing), there was a significant reduction in its population size at the end of the 20th century and it was close to extinction. That is why action plan for species conservation was approved, which included number of management interventions at the locality. The most important was the removal of the upper humus horizon, which aims to create free gaps in the sand that allow the emergence and growth of new plants of D. arenarius subsp. bohemicus. The study species is also attacked by herbivores and it was predicted that increasing population might be endangered with increased herbivore population. The aim of this thesis is therefore to describe the population dynamics of the species, to evaluate the effect of the management and to determine how the herbivory affects the population dynamics of the species. The population growth rate decreases over time with vegetation succession. Even eight years after removal the population is still growing. The population growth rate is most affected by change in...

Anther smuts (Microbotryum violaceum s.l.) represent a well-known system for studying pathogens of wild plants and coevolution between pathogens and hosts. Infected plants produce sterile flowers with anthers filled with fungal spores which are transmitted to other host plants by pollinators. Data from existing research come mainly from the genus Silene. However, the applicability of these findings to other host plants of anther smuts is largely unknown. Therefore, this thesis focuses on a different host species - Dianthus carthusianorum. First, the pattern of disease was surveyed in natural populations of D. carthusianorum in a small area in Střední Povltaví. Most populations were infected to various degrees and only a few populations remained completely healthy. The prevalence of disease was positively correlated with size and density of host populations. On the other hand, environmental factors and the degree of connectedness had very little effect on disease prevalence. Second, additional data were collected from a subset of populations to determine plant resistance and densities of pollinators. Differences in resistance among populations (as inferred from flower inoculations) were not significant. Populations differed in densities of pollinators, but there was no correlation between densities...

Tetínské skály Nature Reserve is one of the major sites for the presence of protected species, which are Dianthus gratianopolitanus, Saxifraga rosacea subsp. Sponhemica and Saxifraga paniculata. My goal was a field survey and data collection about the incidence of these species on the surfaces of rock walls, followed by identification of key environmental factors and description of their impact on the species. Such survey has not been conduncted so far. I used mainly multivariate analysis, PCA and RDA and One-way ANOVA to determine the interrelationships. The analysis results showed significant differences in species abundances influenced by different combinations of environmental factors. The factors with significant influence include cardinal direction. The highest proportion of species abundances showed areas of eastern orientation. Another important factor was shading from vegetation, which proved to have strong negative impact on abundace, especially for species S.rosacea subsp. sponhemica and D. gratianopolitanus. These two variables indicate a preference for sunny areas. Other factors with a significant influence on the occurrence of species are also a presence of soil surface rock wall and a presence of gardens in the area over the rock wall, which greatly increased the proportion of...