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The surface gliding motility of Myxococcus xanthus is required for a multicellular developmental process initiated by unfavorable growth conditions. One form of the M. xanthus surface motility, social (S) gliding, is mediated by the extension and retraction of polarly localized type IV pili (Tfp). Besides Tfp, exopolysaccharides (EPS), another cell surface associated component, are also required for M. xanthus S motility. Previous studies demonstrated that the Dif chemotaxis-like signal transduction pathway is central to the regulation of EPS production in M. xanthus. Specifically, difA, difC and difE mutants were found to be defective in EPS production and S motility. DifA, DifC and DifE, homologous to methyl-accepting chemotaxis proteins (MCPs), CheW and CheA, respectively, are therefore positive regulators of EPS. This study, undertaken to better understand the regulation of EPS production, led to a few major findings. First, DifD and DifG, homologous to CheY and CheC, respectively, were found to be negative regulators of EPS production. Both DifD and DifG likely function upstream of the DifE kinase in EPS regulation. DifB, which has no homology to known chemotaxis proteins, was found not to be involved in EPS production. Secondly, this study led to the recognition that Tfp likely function upstream of the Dif pathway in the regulation of EPS production. Extracellular complementation experiments suggest that Tfp may act as sensors instead of signals for the Dif chemotaxis-like pathway. We propose a regulatory feedback loop that couples EPS production with Tfp function through the Dif signaling proteins. Lastly, we sought to identify additional genes involved in EPS production. Our efforts identified a mutation in a separate chemotaxis gene cluster as a suppressor of difA mutations, suggesting potential cross-talks among the multiple chemotaxis-like pathways in M. xanthus. In addition, we identified twenty-five previously uncharacterized genes that are predicted to be involved in M. xanthus EPS production. These genes appear to encode additional EPS regulators and proteins with biosynthetic function. / Ph. D.

Amoebae are commonly studied to understand embryogenesis, and the best-characterized amoebozoan species is Dictyostelium discoideum (Dd). Dd has a very simple life cycle with a range of developmental stages, among which we are most interested in the stage of a migrating slug. It has been observed that different sizes of Dd slugs maintain a proportional distribution of prestalk cells and prespore cells: prestalk cells occupy the anterior 20% of the slug, while prespore cells occupy the posterior 80%. However, it remains unknown how the migrating slug forms and preserves this anterior-posterior proportional pattern under so many different dynamics including cell movement, signaling, and cell differentiation. Therefore, we constructed a mathematical model to simulate the cell movement and chemical distribution during slug migration, and we conducted numerical experiments to explore possible factors for this pattern. In particular, we divided the problem of interest into the following three parts to be investigated. (1) differential motion: the ability of prestalk cells to move through all the prespore cells and stay in the anterior region of the slug; (2) signaling: how cells of different types produce, receive, and respond to the signals in the environment; (3) cell differentiation: how prestalk and prespore cells differentiate into each other under the regulation of signaling. We finally combined and balanced these mechanisms appropriately to achieve the desired patterns observed in migrating slugs.

Cette recherche s’inscrit dans le cadre de l’évaluation des compétences langagières en français chez des adultes immigrants en vue de leur placement dans des cours de français. Elle porte sur la dimensionnalité, de même que sur la difficulté objective et subjective de tâches discrètes ou intégrées de compréhension écrite, à différents niveaux de maîtrise. Elle propose des analyses de l’estimation de la maîtrise linguistique en fonction de l’appartenance des candidats à des groupes linguistiques distincts. Pour mener à bien la recherche, un test de six textes et de 30 items a été créé. Il a été administré à 118 immigrants. Ces immigrants suivaient les cours de français proposés par le Ministère de l’immigration et des communautés culturelles du Québec (MICC) dans les écoles de langues de l’Université de Montréal et de l’Université du Québec à Montréal. Après administration, ce test a été soumis à des analyses portant sur la dimensionnalité et la difficulté des tâches discrètes et intégrées ainsi que sur les interactions entre ces tâches et les différents groupes de candidats. Des études plus précises ont été faites sur l’interaction entre le type de tâche, l’appartenance à un groupe linguistique pour des candidats et des items de niveau similaire. Enfin, des analyses ont permis d’étudier la perception de la difficulté des tâches par les candidats. L’étude, même si elle porte sur un test en rodage, permet de distinguer la dimensionnalité de tâches discrètes de celle de tâches intégrées. Elle permet également de constater les différences de fonctionnement entre ces deux types de tâches. Enfin, elle permet de comprendre l’interprétation de la difficulté par les candidats et, par ricochet, leur vision du test. In fine, des propositions sont formulées quant à l’opportunité d’utiliser des tâches discrètes et intégrées dans un test de positionnement adaptatif en français langue seconde. / This research has been conducted within the assessment procedure of the language competence of adult immigrants, for placement purposes in French courses. It relates to the dimensionality as well as the objective and subjective difficulty of discrete or integrated reading tasks at different proficiency levels. Analyses of linguistic proficiency estimates are proposed in relation with candidates’ linguistic groups. In order to conduct this study successfully, a 6-text and 30-item test has been constructed and administered to 118 immigrants. These immigrants were enrolled in French courses offered by the Ministry of Immigration and Cultural Communities (MICC) in language schools at the Université de Montreal and the Université du Québec à Montréal. After the administration, analyses have been made on the dimensionality and difficulty of the discrete and integrated tasks and on the interactions between the tasks and different groups of candidates. More detailed analyses have been made on candidates and items at similar levels. Finally, we were able to study the candidates’ perceptions of task difficulty. Although the study is based on a provisional test, the dimensionality of discrete and integrated tasks has been distinguished. Differences in the way the 2 types of task work have been shown. Finally, the candidates’ interpretation of difficulty and therefore, their view of the test, are better understood. In fine, proposals are made in regard with the proper use of discrete and integrated tasks in an adaptive placement test in second language.

The purpose of this investigation was to compare the efficacy of three methods for detecting differential item functioning (DIF). The performance of the crossing simultaneous item bias test (CSIBTEST), the item response theory likelihood ratio test (IRT-LR), and logistic regression (LOGREG) was examined across a range of experimental conditions including different test lengths, sample sizes, DIF and differential test functioning (DTF) magnitudes, and mean differences in the underlying trait distributions of comparison groups, herein referred to as the reference and focal groups. In addition, each procedure was implemented using both an all-other anchor approach, in which the IRT-LR baseline model, CSIBEST matching subtest, and LOGREG trait estimate were based on all test items except for the one under study, and a constant anchor approach, in which the baseline model, matching subtest, and trait estimate were based on a predefined subset of DIF-free items. Response data for the reference and focal groups were generated using known item parameters based on the three-parameter logistic item response theory model (3-PLM). Various types of DIF were simulated by shifting the generating item parameters of select items to achieve desired DIF and DTF magnitudes based on the area between the groups' item response functions. Power, Type I error, and Type III error rates were computed for each experimental condition based on 100 replications and effects analyzed via ANOVA. Results indicated that the procedures varied in efficacy, with LOGREG when implemented using an all-other approach providing the best balance of power and Type I error rate. However, none of the procedures were effective at identifying the type of DIF that was simulated.

Cette recherche s’inscrit dans le cadre de l’évaluation des compétences langagières en français chez des adultes immigrants en vue de leur placement dans des cours de français. Elle porte sur la dimensionnalité, de même que sur la difficulté objective et subjective de tâches discrètes ou intégrées de compréhension écrite, à différents niveaux de maîtrise. Elle propose des analyses de l’estimation de la maîtrise linguistique en fonction de l’appartenance des candidats à des groupes linguistiques distincts. Pour mener à bien la recherche, un test de six textes et de 30 items a été créé. Il a été administré à 118 immigrants. Ces immigrants suivaient les cours de français proposés par le Ministère de l’immigration et des communautés culturelles du Québec (MICC) dans les écoles de langues de l’Université de Montréal et de l’Université du Québec à Montréal. Après administration, ce test a été soumis à des analyses portant sur la dimensionnalité et la difficulté des tâches discrètes et intégrées ainsi que sur les interactions entre ces tâches et les différents groupes de candidats. Des études plus précises ont été faites sur l’interaction entre le type de tâche, l’appartenance à un groupe linguistique pour des candidats et des items de niveau similaire. Enfin, des analyses ont permis d’étudier la perception de la difficulté des tâches par les candidats. L’étude, même si elle porte sur un test en rodage, permet de distinguer la dimensionnalité de tâches discrètes de celle de tâches intégrées. Elle permet également de constater les différences de fonctionnement entre ces deux types de tâches. Enfin, elle permet de comprendre l’interprétation de la difficulté par les candidats et, par ricochet, leur vision du test. In fine, des propositions sont formulées quant à l’opportunité d’utiliser des tâches discrètes et intégrées dans un test de positionnement adaptatif en français langue seconde. / This research has been conducted within the assessment procedure of the language competence of adult immigrants, for placement purposes in French courses. It relates to the dimensionality as well as the objective and subjective difficulty of discrete or integrated reading tasks at different proficiency levels. Analyses of linguistic proficiency estimates are proposed in relation with candidates’ linguistic groups. In order to conduct this study successfully, a 6-text and 30-item test has been constructed and administered to 118 immigrants. These immigrants were enrolled in French courses offered by the Ministry of Immigration and Cultural Communities (MICC) in language schools at the Université de Montreal and the Université du Québec à Montréal. After the administration, analyses have been made on the dimensionality and difficulty of the discrete and integrated tasks and on the interactions between the tasks and different groups of candidates. More detailed analyses have been made on candidates and items at similar levels. Finally, we were able to study the candidates’ perceptions of task difficulty. Although the study is based on a provisional test, the dimensionality of discrete and integrated tasks has been distinguished. Differences in the way the 2 types of task work have been shown. Finally, the candidates’ interpretation of difficulty and therefore, their view of the test, are better understood. In fine, proposals are made in regard with the proper use of discrete and integrated tasks in an adaptive placement test in second language.

This study formed part of a larger project that is concerned with the adaptation of a test of cognitive academic language proficiency, the Woodcock Muñoz Language Survey (WMLS). The WMLS has been adapted from English into isiXhosa and the present study is located within the broader study that is concerned with establishing overall equivalence between the two language versions of the WMLS. It was primarily concerned with the Verbal Analogies (VA) scale. Previous research on this scale has demonstrated promising results, but continues to find evidence of some inequivalence. This study aimed to cross-validate previous research on the two language versions of the WMLS and improve on methodological issues by employing matched groups. It drew upon an existing dataset from the larger research project. The study employed a monolingual matched two-group design consisting of 150 mainly English speaking and 149 mainly isiXhosa learners in grades 6 and 7. This study had two sub aims. The first was to investigate item bias by identifying DIF items in the VA scale across the isiXhosa and English by conducting a logistic regression and Mantel-Haenszel procedure. Five items were identified by both techniques as DIF. The second sub aim was to evaluate construct equivalence between the isiXhosa and English versions of the WMLS on the VA scale by conducting a factor analysis on the tests after removal of DIF items. Two factors were requested during the factor analysis. The first factor displayed significant loadings across both language versions and was identified as a stable factor. This was confirmed by the Tucker’s Phi and scatter plot. The second factor was stable for the English version but not for the isiXhosa version. The Tucker’s phi and scatter plot indicated that this factor is not structurally equivalent across the two language versions / Magister Artium (Psychology) - MA(Psych)

River mouths are very active coastal regions. Continuous sediment supply by the river and the movement by wave action cause the shoreline to change in time and space. Modeling of shoreline changes is an essential step before the design of any coastal engineering project. This research aimed to develop a system of numerical models to present the shoreline changes around a river mouth. The system of numerical models has three components: 1) modeling of nearshore wave characteristics, 2) modeling of longshore sand transport rates using the results of the first component, 3) modeling of shoreline changes using the estimated sand transport rates. Thus, firstly, deep water wave characteristics including the annual wave rose affecting Manavgat River mouth have been obtained from the database of NATO TU-WAVES Project. Then REF/DIF1 and SWAN nearshore wave models have been used to find out nearshore wave conditions. Since the results obtained from REF/DIF1 wave model have been found to be more reasonable compared to SWAN&rsquo / s output, REF/DIF1 wave model has been used in preparing a time series nearshore reference wave file with three hours time interval. This reference file has been used to run GENESIS. Last step of the numerical shoreline change modelling of Manavgat River mouth was the calibration procedure in which the &ldquo / transport parameters&rdquo / k1 and k2 have been determined. As there is lack of measurements of shoreline positions that can be used in calibrating shoreline change model, k1 and k2 has been approximately found to be k1=0.516 and k2=0.9 by using an empirical sediment transport formula. As a future study, it is recommended that when the protection structure controlling the river mouth is finished, the measurements of shoreline position behind the structure should be used in verification of shoreline change model in order to get more accurate results.

Validity is a crucial part of all forms of measurement, and especially in instruments that are high-stakes to the test takers. The aim of this thesis was to examine theory and validity evidence for a recently revised large-scale instrument used for selection to higher education in Sweden, the Swedish Scholastic Assessment Test (SweSAT), as well as identify threats to its validity. Previous versions of the SweSAT have been intensely studied but when it was revised in 2011, further research was needed to strengthen the validity arguments for the test. The validity approach suggested in the most recent version of the Standards for education and psychological testing, in which the theoretical basis and five sources of validity evidence are the key aspects of validity, was adopted in this thesis. The four studies that are presented in this thesis focus on different aspects of the SweSAT, including theory, score reporting, item functioning and linking of test forms. These studies examine validity evidence from four of the five sources of validity: evidence based on test content, response processes, internal structure and consequences of testing. The results from the thesis as a whole show that there is validity evidence that supports some of the validity arguments for the intended interpretations and uses of SweSAT scores, and that there are potential threats to validity that require further attention. Empirical evidence supports the two-dimensional structure of the construct scholastic proficiency, but the construct requires a more thorough definition in order to better examine validity evidence based on content and consequences for test takers. Section scores provide more information about test takers' strengths and weaknesses than what is already provided by the total score and can therefore be reported, but subtest scores do not provide additional information and should not be reported. All four quantitative subtests, as well as the Swedish reading comprehension subtest, are essentially free of differential item functioning (DIF) but there is moderate DIF that could be bias in two of the four verbal subtests. Finally, the equating procedure, although it appears to be appropriate, needs to be examined further in order to determine whether it is the best practice available or not for the SweSAT. Some of the results in this thesis are specific to the SweSAT because only SweSAT data was used but the design of the studies and the methods that were applied serve as practical examples of validating a test and are therefore likely useful to different populations of people involved in test development, test use and psychometric research. Suggestions for further research include: (1) a study to create a more clear and elaborate definition of the construct, scholastic proficiency; (2) a large and empirically focused study of subscore value in the SweSAT using repeat test takers and applying Haberman’s method along with recently proposed effect size measures; (3) a cross-validation DIF-study using more recently administered test forms; (4) a study that examines the causes for the recurring score differences between women and men on the SweSAT; and (5) a study that re-examines the best practice for equating the current version of the SweSAT, using simulated data in addition to empirical data.

Magister Psychologiae - MPsych / In many countries defined by multilingualism, language has been identified as a great influence during psychological and educational testing. In South Africa (SA), factors such as changes in policies and social inequalities also influence testing. Literature supports the translation and adaptation of tests used in such contexts in order to avoid bias caused by language. Different language versions of tests then need to be evaluated for equivalence, to ensure that scores across the different language versions have the same meaning. Differences in dialects may also impact on the results of such tests.Results of an isiXhosa version of the Woodcock Muñoz Language Survey (WMLS),which is a test used to measure isiXhosa learners’ language proficiency, show significant mean score differences on the test scores across rural and urban firstlanguage speakers of isiXhosa. These results have indicated a possible problem regarding rural and urban dialects during testing. This thesis evaluates the item bias of the subtests in this version of the WMLS across rural and urban isiXhosa learners. This was accomplished by evaluating the reliability and item characteristics for group differences, and by evaluating differential item functioning across these two groups on the subtests of the WMLS. The sample in this thesis comprised of 260 isiXhosa learners from the Eastern Cape Province in grade 6 and grade 7, both males and females. This sample was collected in two phases: (1) secondary data from 49 rural and 133 urban isiXhosa learners was included in the sample; (2) adding to the secondary data, a primary data collection from 78 rural isiXhosa learners was made to equalise the two sample groups. All ethical considerations were included in this thesis. The results were surprising and unexpected. Two of the subtests in the WMLS showed evidence of scalar equivalence as only a few items were identified as problematic. However, two of the subtests demonstrated more problematic items. These results mean that two subtests of the WMLS that demonstrated evidence of scalar equivalence can be used to measure the construct of language proficiency, while the other two sub-tests that showed problematic items need to be further investigated, as the responses given by learners on these items seem to be determined by their group membership and not by their ability.