The purpose of this study was to examine students' experiences and perceptions of multiple interaction activities (self-directed, peer, and teacher feedback) implemented in a large multilevel EFL writing class in one private technological university in the southern part of Taiwan. Large size writing classes, quite common in private institutions of higher education in Taiwan, cannot be effectively operated to meet individual students' needs in improving their writing performance. Low achievers have difficulties in keeping up with competent writers in learning writing skills while advanced students complain of their learning too little from the class. This research, based on the activity system model proposed by Engestrom (1987), was a case study in which interviewing student participants, observing classroom activities, audiotaping peer response sessions, and examining students' drafts and feedback sheets were the methods to collect data. The qualitative software, ATLAS.ti, was employed to analyze interview and peer response data according to the code lists developed for this purpose. A rubric was developed to examine the changes students made after having incorporated the three types of feedback into their drafts. Major findings indicated that intermediate and low achievers, though making more efforts in conducting self-directed feedback, felt unsatisfied with this activity while high achievers, investing less energy and time, gave more positive opinions to this activity. However, intermediate and low achievers gave a higher percentage of satisfaction to peer response activities than high achievers because the former could obtain more constructive peer feedback than the latter. In addition, all students were in favor of modified teacher feedback but gave negative opinions to traditional teacher feedback. On the whole, intermediate and low achievers, based on their preference, ranked teacher feedback the most important, then peer feedback and finally self-directed feedback whereas high achievers placed teacher feedback first, self-directed feedback second, and peer feedback last. Student writers' responses to each type of feedback were closely related to the amount of constructive comments they received. The more helpful suggestions they obtained, the more positive opinions they gave to a certain type of feedback. In the end of the study, recommendations were made for curriculum designers, classroom practitioners, and further studies.

Transparent electrodes (TEs) require materials that have both transparency and electrical conductivity, a combination not usually found in nature. They are in increasing demand for use in solar cells, touch screens, displays, transparent heating films and several other devices. Most TEs used today are made of indium tin oxide (ITO). However, it has several disadvantages, such as high fabrication cost, rigidity and brittleness. Many ITO alternatives are being pursued, among which metallic nanowire (NW) networks on transparent substrates such as glass or polymer, have received much attention. This thesis demonstrates ordered silver NW networks on polyimide, fabricated by the field-directed chaining technique. We achieved a sheet resistance of 27 Ω/sq and 95.4% transparency at 550nm, with a Figure of Merit (FOM) 0.023Ω-1, which is higher than the FOM of commercial ITO, 0.005Ω-1. We have demonstrated that ordered NW networks, directed by alternative current (AC) electric fields, are easy to fabricate over a large area and at low cost, on rigid and flexible substrates. The AC electric field changes with different experiment setup. In this work, the effect of polymer thickness, electric field frequency, and gap size between electrodes are explored by COMSOL simulation and validated experimentally. By choosing the appropriate frequency and gap size, ordered NW networks are successfully created on a 23μm polyethylene terephthalate (PET) sheet. Fluid motion is one of the disruptors during NW chaining. We demonstrate control of this disruptor by the use of sandwiched channels for the NW suspension. Post-fabrication treatments are important and necessary for improving the connectivity and conductivity of Ag NW networks. In this work, we explore Joule heating and show its potential to improve the conductivity over other post-treatment approaches. However, Joule heating can also cause failures of NW networks. Ordered NW networks present better optical-electrical properties than random NW networks. Post-fabrication treatment can improve the properties, but there is a limit. In this work, a mathematical model is built for optical-electrical properties of perfectly ordered NW networks, which sets the upper bound of performance for transparent electrodes made of NW networks. A linear relationship is found between the transmittance and inverse sheet resistance. The model is then modified with factors to account for departure from the ideal. / Graduate / 2019-12-12

The role of language has been identified as crucial in the cognitive development of young children, and has been observed on different time-scales. In particular, the real-time verbal mediation of behaviour has been studied in the context of private speech use and self-regulation, pioneered by Vygotsky and continued by others who followed this line of research. However previous studies have mainly attempted to find correlations between speech and self-regulatory behaviour, but have been unable to capture the dynamic and real-time temporal interactions between these phenomena. Hence, without being able to carry out a contextual analysis of the actual instances of temporal co-occurrence between speech and behaviour, correlational analysis is limited in determining the various kinds of verbal mediation that children spontaneously employ as strategies during problem-solving and while exercising self-regulation. The current study proposes ‘temporal pattern analysis’ as an effective method of extracting significantly recurring patterns of task-relevant speech and goal-directed behaviour, as they repeatedly occur in a stream of naturalistic behaviour which may also contain other temporally random events. These recurring temporal patterns are then contextually analysed, considering the pragmatic content of the speech involved and the goal-directedness of the behaviour towards a specific goal of the episode. Goal-directed episodes of behaviour in eight typically-developing preschool children were video-recorded during their self-initiated activities in the classroom as well as during a problem-solving task held in a laboratory setting. The proposed method of temporal and contextual analysis was used to examine the role of both private as well as social speech in the verbal mediation of self-regulatory behaviour during goal-attainment. A Contextual Model of Verbal Mediation was proposed in the study to account for the diverse functions that both social and private speech perform during verbal mediation of one’s own and others’ behaviour in a goal-directed setting, depending on the specific social and task-related context. A dynamic framework of assessment of performance was developed in the study, to account for both successful attempts at self-regulation as well as failures of self-regulation. The study also attempted to determine any consistent group differences in the styles of verbal mediation employed by the children, across the classroom and the laboratory settings.

By applying the principles of Darwinian natural selection in the laboratory, directed evolution has become a powerful practical approach to study enzymes and optimize them to catalyze industrially relevant transformations. In this thesis, I applied this strategy to the engineering of amino acid dehydrogenases for biocatalysis of chiral amines, focusing on two crucial features for successful directed evolution experiments. A first key aspect is the development of technologies allowing the screening of large libraries of enzyme variants to explore sequence space efficiently. Massive scale-down of assay volumes by compartmentalization of library members in water-in-oil emulsions has recently led to the development of ultrahigh-throughput screening platforms that allow sorting of more than 106 variants per hour. So far, these microfluidic droplet sorters have relied exclusively on fluorescent readouts. To further extend the range of applications toward enzymes for which no fluorescent assays are available, I successfully developed a sorting module based on absorbance detection. Using this new module, microdroplets could be sorted based on an absorbance readout at rates of up to 1 million droplets per hour. To demonstrate the utility of this module for protein engineering, three rounds of directed evolution were performed to improve a poorly stable NAD+ dependent phenylalanine dehydrogenase (PheDH) toward its native substrate. Five hits showed increased activity (improved up to 10-fold in lysate; kcat increased >3.5-fold), soluble protein expression levels (>2.5-fold) and thermostability (Tm, 8 °C higher). To increase the sensitivity of the device (3–4 orders of magnitude lower than fluorescence assays) for detection of enzymes with limited stability and low turnovers, an extra step of growth in droplets from single cell encapsulation, followed by piconinection of substrates and lysis agents was implemented. As a result, a fivefold signal enhancement over background was achieved, for an amine dehydrogenase (AmDH) reaction shown to be undetectable in a droplet single cell assay. Second, I investigated how mutational robustness may correlate with protein stability and lead to successful hits after mutagenesis and screening. To examine this issue, I initially investigated various approaches (including ancestral resurrection and computational design) to identify stabilized PheDH variants. One such variant (dubbed Pross 4) showed increased expression levels (>3.3-fold) and thermostability (Tm, 13 °C higher) compared to the wild-type PheDH. I further compared the mutational tolerance and the hit rate between PheDH and Pross 4 by generating variant libraries focused on key active site residues and screening them for improved AmDH activity. The Pross 4 background generated 6.4 times more active variants than the PheDH background, the best hits displaying increased activity (up to 2.5-fold in lysate; kcat/KM increased up to 8-fold) compared to previously engineered AmDHs with the PheDH scaffold. In conclusion, this work highlights how directed evolution experiments could be designed for increased success rates, by combining reliable high-throughput screens with careful choice of evolutionary robust starting points.

Dans l’environnement socio-économique d’une économie de la connaissance naissante et d’un durcissement de la concurrence due au changement d’échelle des échanges, savoir « apprendre » et « entreprendre » semblent devenir une nécessité de survie pour les organisations. Mais c’est sur l’individu lui-même que repose cette double injonction attitudinale. L’Europe souhaite notamment que l’étudiant-e en formation initiale soit capable de faire preuve d’une attitude positive et autonome en apprentissage ainsi que d’une attitude entrepreneuriale. Cette attitude relativement stable et favorable à l’acte d’apprendre, quelle que soit la situation, est appelée « apprenance ». Quant à l’esprit d’entreprendre, il correspond à l’attitude de proactivité. L’auteur commence par valider le caractère opératoire de ces deux instruments de mesure. Puis il étudie de manière quantitative, la manifestation de ces attitudes chez les étudiants de trois filières de spécialisation métier sur deux niveaux d’étude. Il fait alors le constat que d’une certaine indépendance entre l’attitude d’apprenance, qui correspond davantage à une curiosité cognitive teintée d’affectivité, et l’attitude de proactivité, qui correspond à un agir spécifique fait d’anticipation, de contrôle de l’action et de goût pour le défi. Cette recherche conclut que l’échelle d’apprenance complétée par la mesure de l’attitude proactive, permettra de mieux caractériser une disposition à l’apprentissage autodirigé. Ces deux attitudes ont en commun un rapport enthousiaste au changement, une curiosité qui rend disponible envers les opportunités et un effet de renforcement de l’attitude par la réalisation du comportement correspondant. / Faced with the challenges of globalization, Europe wishes to distinguish itself by developing a knowledge society in which the individual becomes a knowledge worker. Within this context, Europe would like that all postgraduate students will be able to learn with autonomy and to act as an entrepreneur in organizations. The analysis we have conducted concerns the study of the correlation between a positive attitude to learning called “learnance” and a positive attitude to entrepreneurial action. This supposes that in the near future, post-graduate students will need to focus on elaborating a lifelong learning strategy, and at the same time manage their career-building projects. After contributing actively to the development and adaptation of two scales measuring the learnance and the Proactive Personality Scale, the author of this research begins by validating the operational nature of these two measuring instruments. Then he makes a quantitative study of the manifestation of these attitudes among students in three areas of specialization on two levels of study.  These observations lead us to note that the attitude of learnance, which can be defined as a cognitive curiosity involving emotion, is distinct from the attitude of proactivity, which implies a specific act involving anticipatory, controlled action and a taste for challenge. However, learnance with the degree of a proactive attitude will enable us to better characterize a readiness to self-directed learning. These two attitudes share an enthusiastic disposition to change, openness towards opportunities and a reinforcing of attitude by the presence of appropriate behaviour.

abstract: The geometric growth in the integrated circuit technology due to transistor scaling also with system-on-chip design strategy, the complexity of the integrated circuit has increased manifold. Short time to market with high reliability and performance is one of the most competitive challenges. Both custom and ASIC design methodologies have evolved over the time to cope with this but the high manual labor in custom and statistic design in ASIC are still causes of concern. This work proposes a new circuit design strategy that focuses mostly on arrayed structures like TLB, RF, Cache, IPCAM etc. that reduces the manual effort to a great extent and also makes the design regular, repetitive still achieving high performance. The method proposes making the complete design custom schematic but using the standard cells. This requires adding some custom cells to the already exhaustive library to optimize the design for performance. Once schematic is finalized, the designer places these standard cells in a spreadsheet, placing closely the cells in the critical paths. A Perl script then generates Cadence Encounter compatible placement file. The design is then routed in Encounter. Since designer is the best judge of the circuit architecture, placement by the designer will allow achieve most optimal design. Several designs like IPCAM, issue logic, TLB, RF and Cache designs were carried out and the performance were compared against the fully custom and ASIC flow. The TLB, RF and Cache were the part of the HEMES microprocessor. / Dissertation/Thesis / Ph.D. Electrical Engineering 2012

abstract: The purpose of this study is to portray kindergarten teachers' developmentally appropriate practices in order to authenticate the essential component of play. Recently, student achievement has been the primary focus in Early Childhood Education, and play is seen as an action that precludes academic learning. This is a qualitative study of teachers' perceptions and teaching practices through observations, interviews, surveys, and journal reflections. The study found that participant kindergarten teachers: (1) have a developing understanding of the positive impact play has on student development, yet they are not aware of how to successfully implement play in their classroom; (2) tend to be more work driven than play driven in their daily activities; and (3) perceive play occurrs when manipulatives are made available for student use, however, the activities are largely teacher-directed in contrast to student initiated play. In summary, participant kindergarten teachers were found to be hesitant to let their control shift to child-initiated learning. There are gaps between teacher knowledge of how child initiated play impacts learning and the actual classroom implementation of child initiated play. Teachers need further development to understand how to use materials to integrate play into daily lessons. It is important to widely disseminate and support the use of Early Childhood National Board Standards regarding play in kindergarten classrooms. Kindergarten teachers require professional development that permits the integration of knowledge of play and the implementation of play in an increasingly accountability driven environment. Keywords: Play; Perceptions of play; Learner-Centered; Developmentally Appropriate Practice (DAP); National Board Certification National Board Certified Teacher (NBCT); National Board for Professional Teaching Standards (NBPTS); English Language Learners (ELL); English Language Development (ELD) / Dissertation/Thesis / Ph.D. Curriculum and Instruction 2012

Les virus oncolytiques sont une nouvelle classe d’agents thérapeutiques pouvant être une alternative au traitement des cancers. Plusieurs virus oncolytiques sont actuellement développés en clinique, néanmoins de nombreuses améliorations sont à apporter afin de créer une nouvelle classe de virus plus efficaces et moins toxiques. Le premier objectif de cette thèse a été d’améliorer la spécificité tumorale du virus de la vaccine via le ciblage de l’antigène MUC1 présenté à la surface des cellules tumorales. Pour cela un virus recombinant présentant à sa surface un fragment d’anticorps (scFv) dirigé contre l’antigène tumoral MUC1 a été construit et produit. Les tests in vitro n’ont toutefois pas permis de mettre en évidence un ciblage spécifique du virus recombinant. Un deuxième aspect de cette thèse a été de tester le potentiel oncolytique de virus de la famille des Poxviridae. Durant ce travail de thèse, les capacités oncolytiques de douze poxvirus, appartenant à 8 genres différents, ont été étudiés. Leurs effets sur la prolifération de cellules cancéreuses humaines ont été évalués. Les virus caractérisés par un effet oncolytique élevé ont été, par la suite, modifiés et armés par ingénierie virale afin d’augmenter leur efficacité. La dernière partie de cette thèse a été consacrée à la génération et la sélection de virus chimériques basées sur la méthode d’évolution dirigée. Cette méthode est utilisée pour mimer le processus naturel de sélection évolutif. Appliqué à la virothérapie oncolytique, ce procédé nous a permis de générer un nouveau virus oncolytique chimérique caractérisé par un potentiel anti-cancéreux amélioré. En résumé, cette thèse a permis, par des techniques d’ingénierie virale, par un criblage de nouveaux virus et par la méthode d’évolution dirigée, de créer et de sélectionner une nouvelle génération de poxvirus oncolytiques présentant une activité thérapeutique accrue avec un profil de toxicité atténué et pouvant être utilisés dans diverses indications thérapeutiques. / Oncolytiques viruses are a new class of therpeutic agents which could be an alternative for cancer treatment. Currently, several oncolytic viruses are evaluated in clinical trial, nevertheless improvements are needed to create a new class of more efficiente and less toxic viruses. The first objective of this thesis was to improved the vaccinia virus specificity through the targeting of the tumor-associated antigen MUC1. To address this goal, a recombinant virus expressing an scFv targeting the MUC1-protein was engineered and produced. However, in vitro, the demonstration of a specific targeting by the recombinant virus was not possible. A second aspect of this thesis work was to evaluate the oncolytic potential of Poxviridae family viruses. Oncolytic capacities of twelve viruses, belonging to eight genera, were evaluated. Their impact on human cancer cells was tested. In order to increase their efficacity, viruses with the highest oncolytic capacities were then modified and armed by genetic engineering. The third part of this work was devoted to the generation of chimeric viruses based on directed evolution process. This methodology is used to mimic the natural process of evolutionary selection. Applied to oncolytic virotherapy, this technique allowed the generation of a new chimeric oncolytic virus caracterised by an enhanced antitumoral potential. In summary, this thesis has allowed, through viral engineering, poxviruses screening and directed evolution methodology, the creation and selection of a new generation of oncolytic poviruses. These viruses demonstrate an increased therpeutic activity and greatest safety profil enabling their application in several therapeutic indication.

Esta dissertação apresenta MagnetViz, uma técnica para visualização de grafos. Enquanto a maior parte das técnicas visualizam um layout de grafo estático pre-computado, MagnetViz permite que usuários dinamicamente alterem o layout de um grafo de forma a melhor satisfazer suas necessidades. Isso é feito ao construir em cima da metáfora de física de algoritmos dirigidos à força para proporcionar aos usuários imãs virtuais, que podem atrair nodos que satisfazem um conjunto de critérios associados a eles. Critérios podem ser baseados na topologia ou semântica do grafo. Através de boundary shapes, que são simples formas geométricas que podem ser colocadas ao redor de imãs, usuários podem também definir regiões na cena onde os nodos atraídos devem permanecer. Grafos são descritos usando GraphML, uma linguagem baseada em XML, que permite a especificação dos nodos e arestas e de atributos para essas entidades. Após a submissão de um grafo como entrada, MagnetViz o exibe utilizando uma versão modificada do algoritmo clássico de Fruchterman and Rheingold, e permite que usuário, a seguir, insira imãs na cena. Usuários podem construir as condições associadas aos imãs utilizando os atributos dos nodos e arestas, além de atributos topológicos próprios de grafos. Para a avaliação de MagnetViz, foi primeiro analisado o desempenho da técnica ao ajudar usuários a executarem tarefas definidas por uma taxonomia de tarefas de visualização de grafos encontrada na literatura. Então, MagnetViz foi testada em um contexto prático através de um estudo de caso. Uma rede de co-autorias foi escolhida como conjunto de dados e o protótipo de MagnetViz foi inicialmente usado para responder questões relevantes a esses dados e então testado por um grupo de potenciais usuários, que tinham de usa-lo para responder essas mesmas perguntas. Após testar a aplicação, os sujeiotos receberam questionários sobre usas opiniões quanto a usabilidade, aplicabilidade, relevância e resultados visuais da técnica. Enquanto alguns aspectos da técnica ainda podem ser melhorados, os resultados da avaliação provaram que MagnetViz é uma abordagem válida para interação com visualizações de grafos. / This dissertation presents MagnetViz, a technique for the visualization of graphs. While most techniques visualize a static pre-computed graph layout, MagnetViz allows users to dynamically alter the layout of a graph to better satisfy their needs. This is done by building on the physics metaphor of force-directed algorithms to provide users with virtual magnets, which can attract nodes that fulfill a set of criteria associated with them. Criteria can be based on either the topology or semantics of the graph. Through boundary shapes, which are simple geometric shapes that can be placed around magnets, users can also define regions within the scene where the attracted nodes should remain. Graphs are described in GraphML, a XML-like description language which allows the specification of nodes and edges between nodes as well as attributes associated to nodes and edges. After loading a graph, Magnetviz displays it using a slightly modified version of the classical Fruchterman and Reingold' algorithm, and allows the user to insert magnets. Users can build the criteria associated with the magnets using the attributes of nodes and/or edges, besides the common graphs' topological attributes. For MagnetViz's evaluation, it was first analyzed how the technique fared in aiding users to perform tasks defined by a graph visualization task taxonomy described in the literature. Then, MagnetViz was tested within a practical context by means of a case study. A co-authorship network was chosen as the target dataset. The MagnetViz prototype was initially used to answer questions relevant to this dataset and then tested by a group of potential users, who had to use it to answer these same questions. After trying the application, subjects answered questionnaires about their opinion on the technique's usability, applicability, relevance and visual results. While some aspects of the technique should still be refined, results of the evaluation proved MagnetViz to be a valid approach when it comes to interaction with graph visualizations.

Este trabalho consiste em um estudo de caso que procurou analisar instrumentos de comunicação dirigida acionados na rede de cooperação empresarial Associação Sul Móveis e Eletrodomésticos - Redlar. O método foi indutivo e a principal técnica de pesquisa utilizada foi a análise de conteúdo, aplicada nos documentos gravados do CD-R: Estatuto Social, Código de Ética, Regimento Interno, Manual de Expansão e Manutenção, e Manual de Identidade Visual, entregue ao novo associado no momento do ingresso na rede. Após a leitura flutuante destes, foram identificados os índices Padrões na Comunicação, Práticas de Gestão, Normativas Comportamentais e Diretrizes Compartilhadas, os quais constituem-se das categorias logomarca, slogan, atividades presenciais, procedimentos operacionais, deveres e obrigações, direitos, penalidades e punições, relacionamentos, objetivos, missão, visão e princípios. O resultado do estudo demonstra que os significados compartilhados constroem a cultura e a identidade da organização e são disseminados pelo instrumento de comunicação dirigida auxiliar CD-R , objeto de análise deste trabalho. / This work presents a case study that analyzed targeted communication aid tools on the business cooperation network Associação Sul Móveis e Eletrodomésticos Redlar. The inductive method was utilized and the main research technique used was content analysis, applied to the documents stored on the CD-R: Social Statute, Code of Ethics, Internal Regiment, Maintenance and Expansion Manual and Visual Identity Manual, given the new associated at the time of entry into the network. After the initial reading of these were identified indices Standard in Communication, Management Practices, Regulatory Behavior and Shared Guidelines, which are in the categories logo, slogan, present activities, operating procedures, duties and obligations, rights, penalties and punishments, relationships, goals, mission, vision and principles. The result of this work shows that the shared meanings defined the culture and the identity of the organization and are disseminated by means of the targeted communication aid CD-R analyzed in this work.