REST står för “Representational State Transfer” och är en arkitekturstil som har blivit ett populärt val för implementation av serviceorienterade arkitekturer (Haupt, Leymann &amp; Vukojevic-Haupt, 2018). REST är en koordinerad uppsättning av arkitektoniska restriktioner som syftar på att minimera latens och nätverkskommunikation, samtidigt som oberoendet och skalbarheten av komponentimplementering maximeras (Fielding &amp; Taylor, 2002). REST är främst baserat på HTTP (Hypertext Transfer Protocol) och skapandet av ett REST api går att genomföra med hjälp av de flesta programspråken. Ett REST api kan dock utvecklas med hjälp av många olika programspråk och vilket programspråk som ska användas för att få den bästa responstiden finns det i dagsläget väldigt lite forskning om. Detta arbetet kommer att jämföra två olika programspråk för att se vilket programspråk som kan användas för att utveckla ett REST api med den bästa responstiden. De programspråk som kommer att användas är Python och PHP. Resultatet på experimenten är att REST api:et skapat med hjälp av PHP har den bästa responstiden jämfört ett liknande REST api skapat med hjälp av Python. En slutsats kan då dras att ett REST api:et skapat med hjälp av PHP har den bästa responstiden. / <p>Det finns övrigt digitalt material (t.ex. film-, bild- eller ljudfiler) eller modeller/artefakter tillhörande examensarbetet som ska skickas till arkivet.</p>

Conditionner les produits alimentaires, pharmaceutiques et cosmétiques pour les conserver et les transporter a toujours été une nécessité pour des hommes à la fois consommateurs et commerçants. Cette étude, qui relève à la fois de l’histoire et de l’archéologie, souligne le rôle majeur joué par le verre, en Provence, au XVIIIe et au XIXe siècle, comme mode de conditionnement des marchandises de qualité. Depuis l’Antiquité jusqu’à nos jours, les produits de luxe, vins fins, huiles surfines et parfums sont protégés et mis en valeur par leur emballage en verre. Il est le matériau idéal car il possède toutes les qualités physiques et chimiques pour devenir fonctionnel et esthétique. Lorsque la Provence se lance, dans les premières décennies du XVIIIe siècle, à la conquête marchande des espaces maritimes, elle bénéficie d’une économie fondée sur la transformation d’agro-alimentaire local ou importé. L’industrie des emballages prend alors un essor considérable en Provence en lien avec cette économie. Les contenants en verre vont ainsi s’exporter à travers le monde, par bateau, grâce à l’action conjuguée de facteurs favorables : le rayonnement international du port de Marseille ainsi que les progrès techniques liés à l’adoption d’une nouvelle source d’énergie, le charbon. Influencée par l’exemple des autres régions, françaises et européennes, la verrerie provençale connaît une métamorphose notable qui conduit à l’abandon progressif des ateliers forestiers au profit d’une implantation portuaire. Sources écrites et iconographiques constituent une grande part de notre documentation complétée par les sources archéologiques. Elles sont liées principalement à la découverte et à l'étude récente d'épaves ainsi qu'à l'inventaire d'un mobilier diversifié, issu des collections publiques et privées. / Packaging food products, pharmaceuticals, and cosmetics to store them and transport them has always been a necessity for humans, both as consumers and merchants. This study, which deals with both history and archeology, highlights the major role played by glass, in Provence, in the 18th and 19th centuries, as a packaging quality method. Since Antiquity, and until the present day, luxury products, fine wines, delicate olive oils, and perfumes have been protected and emphasized through their glass packaging. It is the ideal material because it has all of the necessary physical and chemical properties to become functional and aesthetic. When Provence set out, in the early decades of the 18th century, in merchant conquest of the seas, it had an economy based on the transformation of local or imported agricultural goods. The manufacture of packaging then saw a considerable rise in Provence tied to this economy. Glass containers were then exported throughout the world, by sea, thanks to the combined action of favorable factures: the international reputation of the port of Marseille as well as the technical progress tied to the adoption of a new energy source : coal extraction. Influenced by the example of other regions, both French and European, Provencal glassworks underwent a notable metamorphosis which led to the gradual abandonment of forest glasshouse for port locations. Written and iconographic sources constitute a large part of our documentation supplemented by field sources. These are mainly tied to the discovery and recent study of shipwrecks as well as the survey of a diversified archeological inventory, derived from public and private collections.

Bioreactors are devices used for the growth of tissues in a laboratory environment. They exist in many different forms, each designed to enable the production of high-quality tissues. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. Chondrocytes, the building blocks of articular cartilage, for example, are stimulated by mechanical stresses such as shear, as compared with those in tissues grown under static incubation conditions. On the other hand, high shear can damage cells. Consequently the shear-stress level has to be controlled in order to optimize the design and the operating conditions of bioreactors. Spinner flasks have been used for the production of articular cartilage in vitro. Assuming the existence of a relation between the cellular glycosaminoglycan (GAG) synthesis and the local shear stresses on the construct surfaces, this research focuses on the development of a model for cartilage growth in such devices. The flow produced in a model spinner flask is characterized experimentally using particle-image velocimetry (PIV). A computational fluid dynamic (CFD) model validated with respect to the laboratory measurements is constructed in order to predict the local shear stresses on the construct surfaces. Tissue growth experiments conducted in the prototype bioreactor permit construct histologies and GAG contents to be analyzed and then correlated with the shear-stress predictions. The integration of this relation into the CFD model enables the prediction of GAG synthesis through convective effects. Coupling this convective model to an existing diffusive model produces a complete cartilage-growth model for use in aiding the optimization of existing bioreactors, and in the design of new ones.

The objective of this thesis was to design and implement a scalable and load efficient back-end system for web game services. This is of interest since web applications may overnight gain a significant increase in user base, because of viral sharing. Therefore designing the web application to service an increasing amount of users can make or break the application, in regard to keep the user base. Because of this, testing how well the system performs during heavy load can be used as a foundation when making a decision of when and where to scale up the application. The system was to be generically accessible through an Application Programming Interface (API) by the different game services. This was done using a RESTful architecture where emphasise was put on building the system scalable and load efficient. This thesis focuses on designing and implementing such a system, and how load testing can be used to evaluate this systems performance for an increasing amount of simultaneous clients using the web application. The results from load testing the implemented system was above the expectations, considering the hardware used when running the tests and hosting the system. The conclusion of this thesis is that by following REST when designing a web service, scalability becomes a natural part of how one would design the system.

Abstract Small-scale cultivation methods are a necessity for the development of new biotechnological processes. The most common method for submerged microbial cultivation is a shake flask used with a batch operation protocol. Well plate cultivation formats have also increased their importance, due to the need to utilize high-throughput cultivations for efficient product development. However, batch cultivation is often not the optimal method for obtaining high cell densities and good product quality, due to unlimited microbial growth. The aim of this dissertation was to improve small-scale microbial cultivations for microbial growth and product formation. Hydrolytic enzymes were utilized to relieve nutrient limitation by hydrolysis of proteins in lactic acid bacteria cultures to improve lactic acid production from dairy side products. Hydrolytic enzymes were also utilized in the enzymatic release of glucose from starch to create a fed-batch-like cultivation system applicable on small scale. The wireless sensor system developed was applied in shake flask cultivations to monitor oxygen and pH levels. Enzymatic polymer processing was applicable for small-scale cultivations. Lactic acid production by Lactobacillus salivarius ssp. salicinius was enhanced four-fold when the proteins were hydrolyzed either by proteases or by proteolytic microbes. The fed-batch-mimicking controlled glucose feeding and growth control were obtained by means of the simultaneous enzymatic hydrolysis of starch-polymer during cultivation. Controlled growth, higher cell densities, decreased side product formation and increased amount of soluble protein product were obtained in Escherichia coli cultivations. When this method was applied to the cultivation and recombinant protein production of the methylotrophic yeast Pichia pastoris, higher cell densities and higher amounts of active protein were obtained. The glucose concentration remained low enough to avoid the substrate repression of the alcohol oxidase promoter. The fed-batch method is suitable for high-throughput cultivations since the method can be utilized in well plate formats without external feeding devices. The method can be utilized in the development of new biotechnological products, especially when the production system is sensitive to growth conditions, and growth control is preferred. / Tiivistelmä Pienen mittakaavan mikrobikasvatusmenetelmiä tarvitaan kehitettäessä uusia bioteknologisia prosesseja. Tavallisin menetelmä mikrobien liuoksessa tapahtuvaan kasvatukseen on panostyyppisesti tehtävä sekoituspullokasvatus. Kuoppalevykasvatukset ovat myös tulleet entistä tärkeämmiksi, koska tuotekehityksen tehostamiseksi on tarvetta käyttää high-throughput-menetelmiä. Tavoiteltaessa korkeita mikrobisolutiheyksiä ja tuotteen hyvää laatua, panostyyppinen kasvatus ei ole usein paras vaihtoehto, johtuen mikrobien rajoittamattomasta kasvusta. Tämän työn tarkoituksena oli parantaa mikrobien kasvua ja tuotteen muodostusta pienen mittakaavan kasvatuksissa. Meijeriteollisuuden sivutuotteiden proteiineja pilkottiin entsyymien avulla, jotta maitohappobakteerit pystyivät hyödyntämään proteiinit tehokkaammin ja tuottamaan enemmän maitohappoa. Hydrolyyttisiä entsyymejä hyödynnettiin myös glukoosin vapauttamiseen tärkkelyksestä, jolloin saatiin luotua pieneen mittakaavaan sopiva panossyöttötyyppinen kasvatusmenetelmä. Työn aikana kehitettyä langatonta mittausjärjestelmää hyödynnettiin sekoituspullokasvatuksissa happipitoisuuden ja pH:n seurantaan. Entsymaattinen polymeerien käsittely oli soveltuva menetelmä pienen mittakaavan kasvatuksiin. Maitohapon tuotto Lactobacillus salivarius ssp. salicinius -mikrobilla nelinkertaistui, kun ravinneproteiinit pilkottiin joko proteaasien tai proteolyyttisten mikrobien avulla. Panossyöttömenetelmää muistuttava hallittu glukoosin syöttö ja mikrobin kasvun hallinta saavutettiin pilkkomalla tärkkelystä glukoosiksi kasvatuksen aikana. Escherichia coli kasvatuksissa saavutettiin hallittu solumäärän kasvu, korkeammat solutiheydet, vähentynyt sivutuotteiden muodostus ja suurempi liukoisen tuoteproteiinin määrä. Tätä menetelmää sovellettiin myös vierasproteiinin tuottoon metylotrofisella Pichia pastoris -hiivalla, jolloin saavutettiin korkeammat solutiheydet ja suurempi aktiivisen tuoteproteiinin määrä. Glukoosin määrä kasvatusliuoksessa pysyi riittävän alhaisena, jotta se ei repressoinut hiivan alkoholioksidaasi-promoottoria. Panossyöttömenetelmä on sopiva high-throughput-mikrobikasvatuksiin, koska sitä voidaan käyttää kuoppalevyillä ilman syöttölaitteita. Menetelmää voidaan hyödyntää uusien bioteknisten tuotteiden kehittämisessä erityisesti silloin, kun tuottoisäntä on herkkä kasvuolosuhteiden suhteen ja mikrobin kasvua halutaan hallita.

Plants produce a variety of secondary metabolites, e.g. to defend themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation in a modern shake flask system with non-invasive online respiration activity monitoring unit. Comprehensive screening experiments confirm the successful transfer of a model culture (sunflower suspension) into the shake flask monitoring device and the suitability of this respiration activity monitoring unit as qualified tool for screening of plant in vitro cultures (sunflower and sage suspension). The authors demonstrate deviations between online and offline data due to varying water evaporation from different culture flask types. The influence of evaporation on growth-specific parameters thereby rises with increasing cultivation time. Furthermore, possibilities to minimize the impact of evaporation, either by adjusting the inlet air moisture or by measuring the evaporation in combination with an appropriate correction of the measured growth values, are shown.

Plants produce a variety of secondary metabolites, e.g. to defend themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation in a modern shake flask system with non-invasive online respiration activity monitoring unit. Comprehensive screening experiments confirm the successful transfer of a model culture (sunflower suspension) into the shake flask monitoring device and the suitability of this respiration activity monitoring unit as qualified tool for screening of plant in vitro cultures (sunflower and sage suspension). The authors demonstrate deviations between online and offline data due to varying water evaporation from different culture flask types. The influence of evaporation on growth-specific parameters thereby rises with increasing cultivation time. Furthermore, possibilities to minimize the impact of evaporation, either by adjusting the inlet air moisture or by measuring the evaporation in combination with an appropriate correction of the measured growth values, are shown.

Perfluorinated compounds are organic compounds in which all hydrogen atoms in a carbon chain are substituted with fluorine atoms. These compounds are highly stable, persistent and bioaccumulative. They are purely anthropogenic compounds contained in biota and abiota. Partition coefficient between n-octanol and water is the essential toxicological parameter of a compound. This parameter helps us to assess behaviour of compound in the environment as well as in the living organisms. The shake flask method and the RP-HPLC method were employed to measure the Kow of nine perfluorinated acids. Using the shake flask method, the surface activity of compounds and the acid dissociation caused false results of the measurement. But behaviour of these compounds in the environment can be assess. Accurate and precise results were measured by the RP-HPLC method using an acetate buffer. Log Kow of perfluoro- carboxylic acids, with the carbon chain length of 5-14, were found out, their final value ranging between 1.66 and 5.10. Log Kow of acid with 12 carbons was estimated based on the linear regression of dependence of log Kow on the number of carbons. There were significant differences in the results obtained by various software. Thus, the results cannot be considered relevant. These software are not suitable for...

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