Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-04-25T14:06:01Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_joelma_roberta_goncalves.pdf: 1590020 bytes, checksum: 0e2f166f0a45431a2ae95210f70078f8 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-25T17:17:30Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_joelma_roberta_goncalves.pdf: 1590020 bytes, checksum: 0e2f166f0a45431a2ae95210f70078f8 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-25T17:18:32Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_joelma_roberta_goncalves.pdf: 1590020 bytes, checksum: 0e2f166f0a45431a2ae95210f70078f8 (MD5) / Made available in DSpace on 2017-04-25T17:18:49Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_joelma_roberta_goncalves.pdf: 1590020 bytes, checksum: 0e2f166f0a45431a2ae95210f70078f8 (MD5) Previous issue date: 2015-06-02 / Sem bolsa / No Brasil, a área cultivada com milho está em torno de 12 milhões de hectares. Apesar dessa cultura possuir um alto potencial de produtividade, é diretamente afetada pelo ataque de várias espécies de insetos desde a semeadura até o armazenamento. Dessa forma, este trabalho teve como objetivo a comparação de cultivares de milho transgênico e não transgênico, com base nos níveis de migrações populacionais das ninfas do percevejo castanho-das-raízes S. carvalhoi em teste com oportunidade de escolha. Três variedades de plantas foram estabelecidas, em um conjunto formado por seis recipientes circulares de PVC, interligados simetricamente com livre comunicação a um recipiente central circular. O experimento foi organizado segundo o delineamento inteiramente casualizado e constou de três tratamentos e oito repetições. A avaliação do ensaio foi realizada no 60o dia após a liberação das ninfas, por meio da contagem de insetos que migraram para as raízes das diferentes plantas. Demonstrou-se que, das ninfas de 1o, 2o, 3o e 4o estádios, respectivamente, 17,5; 23,33; 34,16 e 19,16% optaram por alguma cultivar de milho em estudo. Constatou-se que, 22,5; 30,0 e 0,0% de ninfas de 1o estádio, optaram por, respectivamente, plantas da variedade Dekald 390 YG, Syngenta Impacto (convencional) e plantas da variedade Pioneer P30F53 YG; 37,5; 27,5 e 5,0% das ninfas de 2o estádio, optaram por, respectivamente, plantas da variedade Dekald 390 YG, plantas da variedade Syngenta Impacto (convencional) e plantas da variedade Pioneer P30F53 YG; 52,5%, 35,0% e 15,0% de ninfas 3o estádio, optaram por, respectivamente, convencional, Dekald 390 e Pioneer P30F53 YG e 27,5; 15,0 e 15,0% de ninfas de 4o estádio, optaram por, respectivamente, plantas da variedade Dekald 390 YG, plantas da variedade Syngenta Impacto (convencional) e plantas da variedade Pioneer P30F53 YG. As ninfas de primeiro, segundo, terceiro e quarto estádios se concentraram em maiores quantidades nas plantas de variedades convencionais e Dekald 390 YG e em menores concentrações nas plantas da variedade Pioneer P30F53 YG. Das três variedades de milho avaliadas, a cultivar Pioneer P30F53 YG foi a que obteve a menor preferência (IP) pelas ninfas de primeiro, segundo e terceiro estádios. Conclui-se que o hibrido de milho da variedade Pioneer F53YG demonstra não ser atrativo as ninfas do S. carvalhoi, apresentando potencial para uso em áreas infestadas. Os híbridos Dekalb390 YG e Syngenta Impacto (convencional) são atrativos ao percevejo castanho das raízes S. carvalhoi, não sendo recomendado o cultivo em áreas infestadas pelo inseto. / The area under maize is around 12 million hectares in Brazil. However, this crop has a high yield potential, it may be directly affected by the attack of several insects from planting to its storage. This study aimed to compare transgenic maize cultivars and non-GMO, based on the levels of population migration of the brown-roots bug (S. carvalhoi) nymphs in test with opportunity of choice. Three varieties of plants were established in a group of six circular container formed of PVC, symmetrically linked by an open communication with a free passage to a central circular container. The experiment was organized in a completely randomized design and consisted of three treatments and eight repetitions. Evaluation test was conducted on the 60th day after the release of nymphs, by counting insects that have migrated into the roots of different plants. It has been shown that the nymphs of 1st, 2nd, 3rd and 4th stages, respectively, 17.5; 23.33; 34.16 and 19.16% choose for some cultivar studied. It was found that 22.5; 30.0 and 0.0% of nymphs of the first stage, chose respectively Dekald 390 YG, Syngenta Impact (conventional) and Pioneer P30F53 YG. About second stage nymphs 37.5; 27.5 and 5.0%, chose respectively Dekald 390 YG, Syngenta Impact (conventional) and Pioneer P30F53 YG, and 52.5%, 35.0% and 15.0% of third instar nymphs, choose, respectively, conventional, Dekald 390 and Pioneer P30F53 YG and 27.5; 15.0 and 15.0% of nymphs of fourth stage, choose respectively Dekald 390 YG, Syngenta Impact (conventional) and Pioneer P30F53 YG. Nymphs of the first, second, third and fourth stages were concentrated in larger quantities in conventional plant varieties and Dekald YG 390, in lower concentrations in Pioneer P30F53 YG. Pioneer P30F53 YG was the cultivar with the lowest preference (IP) by the nymphs of first, second and third stages. We conclude that corn hybrid variety Pioneer F53YG demonstrates not be attractive to S. carvalhoi nymphs, with potential for use in infested areas. Hybrid Dekalb390 YG and Syngenta Impact (conventional) are attractive to brown bug of roots (S. carvalhoi), not recommended cultivation in areas infested by the insect.

Made available in DSpace on 2014-12-17T14:33:07Z (GMT). No. of bitstreams: 1 FabricioRC_DISSERT.pdf: 838962 bytes, checksum: 3096b614c5fa73cd030f8193624b3469 (MD5) Previous issue date: 2012-08-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Protozoa may be an important alternative food source for Calanoida copepods in these environments. Aiming to quantify the feeding preferences of N. cearensis by ciliates in the presence of cyanobacteria, in vitro experiments were conducted, using mixed cultures in different concentrations of total food for copepod. Two ciliates species (Paramecium sp. and Cyclidium sp.) and a cyanobacteria toxic strain (Microcystis aeruginosa) were offered as food. Previous experiments were done to identify the copepod s maximum ingestion rate through the use of a type II functional response model when each prey is offered separately. High maximum ingestion rate were found when those protists were offered as prey. N. cearensis showed significant preference for protozoal prey over the cyanobacterium tested both in low (corresponding 95.15% of the diet) and in high food concentration treatments (about 91.56% of the diet), preferring the bigger ciliate in lower concentrations (67.52% of the diet). The meaningful involvement of heterotrophic organisms in the zooplankton diet emphasis the microbial loop participation in the energy transition from copepods to higher trophic levels. This data contributes to understand the stability of existing trophic interactions in reservoirs subjected to eutrophication and assists trophic cascade studies in these environments / Protozo?rios podem ser uma importante fonte alternativa de alimento para cop?podos Calanoida em ambientes dominados por cianobact?rias. Com o objetivo de quantificar a prefer?ncia alimentar de Notodiaptomus cearensis por ciliados em presen?a de cianobact?rias, experimentos in vitro foram conduzidos, utilizando-se culturas mistas em diferentes concentra??es totais de alimento para o cop?podo. Duas esp?cies de ciliados (Paramecium sp. e Cyclidium sp.) e uma cepa t?xica de cianobact?ria (Microcystis aeroginosa) foram ofertados como alimentos. Experimentos pr?vios visando identificar as taxas de ingest?o m?xima do cop?podo atrav?s do uso de um modelo de resposta funcional do tipo II quando cada uma das presas ? ofertada separadamente tamb?m foram realizados. O cop?podo apresentou maior taxa de ingest?o m?xima quando ciliados s?o ofertados como presa. N. cearensis mostrou prefer?ncia significativa por protozo?rios em detrimento da cianobact?ria testada tanto em tratamentos com menor (correspondendo a cerca de 95,15% da dieta) quanto com maior concentra??o de alimento (cerca de 91,56% da dieta), preferindo o ciliado de maior tamanho nas menores concentra??es (67,52% da dieta). A participa??o significativa de organismos heterotr?ficos na dieta do zoopl?ncton d? maior ?nfase ? participa??o da al?a microbiana na passagem de energia para n?veis tr?ficos superiores. Tal dado colabora para o entendimento da estabilidade das intera??es tr?ficas existentes em reservat?rios sujeitos a eutrofiza??o e auxilia estudos de cascata tr?fica nesses ambientes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The aim of this work was to evaluate the influence of different seasons on the ingestive behavior, on the botanical composition of the diet of goats grazing in the caatinga and on the availability and quality of forage of the caatinga. Four castrated male goats were used, with a mean weight of 30.2 kg and mean age of 20 months. The experiment had a duration of 12 months, comprising two rainy seasons and one dry season, and the treatments consisted of the three seasons (the two rainy seasons were treated separately) with 3 repetitions per season. Each animal was always accompanied by the same observer. The observed behaviors were feeding, idleness, rumination, displacement, social interaction and water intake. Forage collections were performed at 30 points in the area, considering a maximum collection height of 1.5m. All the forage collected and classified in the different strata (herbaceous, woody and litter), after the analysis was performed to know the bromatological composition of these. Non-parametric tests of Kruskal-Wallis (5%) and Nemenyi (5%) were performed in order to identify differences in the occurrence of behaviors between each season of the year. There was no significant difference (P≥0.05) between rumination, displacement and others (sum of social interaction and water intake) in the different seasons. However, a significant difference was observed in the occurrence of feeding and idleness (P = 0.0015 and P = 0.0001, respectively). In the dry season, a higher feeding occurrence and less occurrence of idleness occurred, contrary to what occurred in the rainy season/18 where there was greater occurrence of idleness and less feeding. The woody stratum presented the best bromatological composition, however the stratum was less present, and the herbaceous stratum was more available during the experimental period. The species whose consumption was recorded were: pinhão (jatropha molissima), velande (Croton heliotropiifolius), catingueira (Poincianella pyramidalis), marmeleiro (Croton blanchetianus), pereiro (Aspidosperma pyrifolium), macambira (Bromelia laciniosa), umburana (Commiphora leptophloeos) maniçoba (Manihot glaziovii), besides the herbaceous stratum, litter and cactaceae, the latter consisting of clusters of species of the same habit of growth. Most of the food in the rainy season/17 consisted of the herbaceous stratum (67.47%), in the dry season consisted of litter (56.12%) and in the rainy season/18 consisted of macambira (61.13%). The seasons influenced the pattern of behavior, the choice of food and the availability and quality of forage found in the caatinga. / O objetivo deste trabalho foi avaliar a influência de diferentes estações no comportamento ingestivo, na composição botânica da dieta de caprinos pastejando na caatinga e na disponibilidade e qualidade de forragem da caatinga. Foram utilizados 4 caprinos machos castrados sem raça definida, com peso médio de 30,2 Kg e idade média de 20 meses. O experimento teve um período de duração de 12 meses, englobando duas estações chuvosas e uma seca, sendo que os tratamentos consistiram nas 3 estações (as duas estações chuvosas foram tratadas de forma separada) com 3 repetições por estação. Cada animal foi sempre acompanhado pelo mesmo observador. Os comportamentos observados foram alimentação, ócio, ruminação, deslocamento, interação social e ingestão de água. Coletas de forragem foram realizadas em 30 pontos na área, considerando uma altura máxima de coleta de 1,5m. Toda a forragem coletada e classificada nos diferentes estratos (herbáceo, lenhoso e liteira), após foi realizada a análise para se conhecer a composição bromatológica destes. Foram realizados os testes não paramétricos de Kruskal-Wallis (5%) e Nemenyi (5%) com o intuito de identificar diferenças na ocorrência dos comportamentos entre cada estação do ano. Não foi observada diferença significativa (P≥0,05) entre a ruminação, deslocamento e outros (soma da interação social e ingestão de água) nas diferentes estações. Contudo, observou-se diferença significativa na ocorrência de alimentação e ócio (P=0,0015 e P=0,0001, respectivamente) sendo que na estação seca se observou maior ocorrência de alimentação e menor ocorrência de ócio, ao contrário do que ocorreu na estação chuvosa/18 onde houve maior ocorrência de ócio e menor alimentação. O estrato lenhoso apresentou a melhor composição bromatológica, contudo foi o estrato menos presente, estando o estrato herbáceo em maior disponibilidade durante o período experimental. As espécies cujo consumo foi registrado foram: pinhão (jatropha molissima), velande (Croton heliotropiifolius), catingueira (Poincianella pyramidalis), marmeleiro (Croton blanchetianus), pereiro (Aspidosperma pyrifolium), macambira (Bromelia laciniosa), umburana (Commiphora leptophloeos), maniçoba (Manihot glaziovii), além do estrato herbáceo, liteira e cactáceas, consistindo estas últimas em conjuntos de espécies de mesmo hábito de crescimento. A maior parte do alimento na estação chuvosa/17 consistiu no estrato herbáceo (67,47%), na estação seca consistiu na liteira (56,12%) e na estação chuvosa/18 consistiu na macambira (61,13%). As estações influenciaram o padrão de comportamento, na escolha dos alimentos e na disponibilidade e qualidade da forragem encontrada na caatinga. / São Cristóvão, SE

Field and laboratory studies were used to elucidate the factors affecting temporal and spatial variations of species abundance and structure of macroalgal assemblage and environmental variables between fish farming (FFA) and non-fish farming (NFFA) areas in Hsiao-Lu-Chiao island, a coral island in southwestern Taiwan. Four experiments have been approached: 1. field surveys of macroalgal assemblage structure on 5-m and 10-m depth at 3 sampling sites at FFA (FFA1, FFA2 and FFA3) and 1 sampling site at NFFA from September 2004, January 2005 and April 2005; 2.the relationship between abiotic (monthly maximum air temperature, monthly minimum air temperature, monthly mean air temperature, monthly cumulative precipitation, monthly cumulative irradiance, seawater temperature, light extinction coefficient, water motion, and nutrient (NO3-, NO2-, NH4+, SRP, DON, and DOP) and biotic (seaurchin density) factors and spatio-temporal variations in macroalgal structures analyzed by non-parametric multivariate model; 3. Factors affecting macroalgal abundance and structure: (1). Comparison of growth temperature ranges in different species to field temperature fluctuation; (2).Comparison of growth irradiance ranges in different species to field irradiance fluctuation; 4.Herbivore pressure: (1). Spatio-temporal variations of sea urchin abundance and structure of assemblage; (2). Gut contents and food preference of sea urchin experiment; (3). Herbivore exclusion experiment. Macroalgal %cover, biomass, species richness, diversity (H¡¦) and evenness (J¡¦) showed temporal and spatial variations, low values in January 2005 and also low values in the 5 m- and 10 m-depth areas of FFA1 and the 10 m-depth areas of FFA2. The data of k-dominance curve, hierarchical cluster and ANOSIM tests indicate that macroalgal assemblage is different between 4 sampling sites, between 2 depths and between 3 seasons. Ceratodictyon spongiosum is the most important species that separates September and January assemblages from April assemblage and separates the FFA1 and FFA2 assemblages from the FFA3 and NFFA assemblages. BVSTEP analysis shows that nutrients (NO3-, DON, DOP), temperature, monthly cumulative precipitation, and sea urchin density are the factors corresponding to variations of macroalgal assemblages, this correlation is more significant for 5 m-depth assemblage. Fish farming area FFA1and FFA2 assemblage are affect by sea urchin density, temperature and DON. Sea urchin influnces macroalgal abundance and assemblage structure in FFA1 and FFA2. Macroalgal %cover in 5 m-depth area shows a reversal relationship with sea urchin density; however, this relationship is not observed for 10 m-depth area. FFA1 and FFA2 are belong to high grazing pressure sites as indicated by high sea urchin density and exclusion experiment. Sea urchin gut contents and feeding preference test show that sea urchin has strong food selectivity with Hypnea charoides and Gracilaria coronopifolia as the most preferred species. Herbivore exclusion experiment shows that Hypnea charoides and Gracilaria coronopifolia are the species recruited in the cages. Ceratodictyon spongiosum had high biomass in FFA1 and FFA2 in January, which was ¡¥low DOP/high DON¡¦. The coindicence of temporal variations in FFA3 assemblage structure with a change from ¡¦Halimeda opuntia and Boodlea compostia¡¦ ¡÷ ¡¦Amphiroa fragilissima, Corallina phhulifera and Galaxaura oblongata¡¦ ¡÷¡¦Halimeda opuntia and Boodlea compostia¡¦ with low nitrogen/ high phosphorous¡¦ ¡÷¡¦ high nitrogen/ low phosphorous¡¦ ¡÷ ¡¦low nitrogen/ high phosphorous¡¦ suggest a role of ¡¦low nitrogen (NO3-)/high phosphorous (DOP)¡¦ for FFA3 structure modification. NFFA assemblage is controlled by temperature and monthly cumulative precipitation. Monthly cumulative precipitation in September was higher than January and April, in which Boodlea compostia and Gracilaria coronopifolia were dominant algae in September. The temperature growth responses of algae using the continuous-flow outdoor laboratory tank culture system fit their seasonal growth, reflecting the temperature-dependent manner of seasonal variations in abundance. It could be concluded from the present investigation that the structure of benthic macroalgal assemblage in Hsiao-Lu-Chiao island in southwestern Taiwan is affected by predicted natural and pulse disturbances. Temperature fluctuations involve in overall temporal variations in structure. Sea urchin herbivory and nutrient as pulse nutrient modulate the structure in fish farming area while monthly cumulative precipitation is associated with algal structure in non-fish farming area.

No description available.

Les chirurgies de type by-pass induisent des améliorations spectaculaires de l'homéostasie glucidique indépendamment de la perte de poids chez les patients obèses et diabétiques. Elles provoquent également une diminution de la préférence pour les aliments hypercaloriques. Un mécanisme proposé pour expliquer les effets sur le contrôle glucidique de ces procédures associe une diminution de la production hépatique de glucose et une augmentation de la néoglucogenèse intestinale. Les acides biliaires, décrits comme inhibiteurs de la néoglucogenèse, voient leur biodisponibilité modifiée après ces chirurgies. Ils sont en effet absents de l'anse alimentaire tandis que leur concentration est augmentée dans le sang. Nous avons donc testé l'hypothèse selon laquelle les acides biliaires plasmatiques inhiberaient la production hépatique de glucose alors que leur absence dans l'intestin induirait la néoglucogenèse intestinale. Pour cela, nous avons réalisé des dérivations biliaires chez le rat visant à reproduire le trajet de la bile dans les chirurgies de type by-pass. Nous avons montré que les dérivations biliaires entrainent une augmentation du taux circulant d'acides biliaires. Les dérivations provoquent une forte diminution de la production hépatique de glucose et une induction de la néoglucogenèse dans les portions d'intestin dépourvus de bile. Par ailleurs, la seule modification du trajet de la bile promeut une amélioration du contrôle glucidique et une diminution de l'appétence pour les aliments riches en graisse et en sucre. Ces données nous ont donc permis d'attribuer un rôle clé à la modification du trajet de la bile dans les effets bénéfiques des chirurgies de type by-pass / Gastric bypass procedures have emerged as an effective treatment for morbid obese diabetic patientssince they provoke a rapid remission of diabetes before any weight loss has occurred. Patients also report adisinterest in high calorie food. A suggested mechanism associated a decrease in hepatic glucose production toan enhanced intestinal gluconeogenesis. Bile acids, described as inhibitors of gluconeogenesis, see theirbioavailability changed after these procedures. Indeed, they are absent in the alimentary limb while theirplasmatic concentration is increased. We therefore tested the hypothesis that plasma bile acids may inhibithepatic glucose production while their absence in the gut could induce intestinal gluconeogenesis.For this, we performed bile diversions matching the modified biliary flow occurring after gastric bypassprocedures. We showed that bile diversions lead to an increase in plasma bile acids. Bile diversions promote ablunting in hepatic glucose production whereas intestinal gluconeogenesis is increased in gut segments devoidof bile. Moreover, the modification of bile routing per se improves glucose control and dramatically decreasefood intake due to an acquired disinterest in fatty food. This data shows that bile routing modification is a keymechanistic feature in the beneficial outcomes of gastric bypass procedures