Inaug.--Diss.--Münster, 1937. / Includes bibliographical references (p. 89-101).

Made available in DSpace on 2014-06-11T19:32:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T20:42:38Z : No. of bitstreams: 1 shinohara_eh_dr_araca.pdf: 207616 bytes, checksum: e65ac1314c6696afd0bddbe254269277 (MD5) / Neste estudo foram avaliados 264 casos de carcinoma espinocelular de gengiva inferior, tratados no Centro de Tratamento e Pesquisa-Hospital do Câncer- A . C. Camargo no período de 1954 a 1995. Em relação aos dados clínicos, houve predomínio do sexo masculino, raça branca e idade entre 46 e 64 anos. Apenas 32 casos (12,16%) foram diagnosticados nos estádios clínicos I e II. O consumo de tabaco, foi encontrado em 77,56% dos casos e 59,69% dos pacientes faziam uso de bebida alcoólica. Na análise univariada de sobrevida global, encontrou-se melhor prognóstico associado aos estádios iniciais, ausência de metástase cervical, sexo feminino e submetidos a tratamento cirúrgico. Na análise multivariada, as variáveis relacionadas ao aumento no risco de óbito, foram sexo masculino e aspecto clínico úlcero-infiltrativo. / In the present study 264 cases of squamous cell carcinoma of lower gingiva treated at Centro de Tratamento e Pesquisa-Hospital do Cancer- A . C. Camargo between 1954 to 1995 were evaluated. Concerning the clinical features, there was a predominance of male, white people age 46 to 65 years. Only 32 cases (12,16%) were diagnosed at clinical stage I and II. 77,56% of the cases were tobacco consumers and 59,69% were alcohol drinkers. In the univariate survival rate, a better prognostic was found related to early clinical stage diagnostic, absence of nodal involvement, female cases, and patients treated by surgery. The features related to a higher risk of death with the multivariate analysis were male patients and infiltractive clinical aspect.

Orientador: Luis Carlos Spolidório / Banca: Benedicto Egbert Corrêa de Toledo / Banca: Carlos Rossa Júnior / Banca: Maria Ângela Naval Machado / Banca: Maria Lúcia Rubo de Rezende / Resumo: Tacrolimus (FK506) é uma droga imunossupressora usada em transplantes de órgãos e também como terapia alternativa em pacientes com rejeição refratária de enxertos ou intolerância à ciclosporina A (CsA), sem os efeitos adversos atribuídos freqüentemente à CsA. Atualmente não há estudos que explorem os efeitos da administração de FK506 por longos períodos sobre o periodonto. Ë sugerido também que terapias imunossupressivas, principalmente com FK506, possam ser um importante fator no desenvolvimento de Diabetes mellitus pós-transplantes (PTDM). Existem alguns estudos explorando os efeitos do FK506 por longos períodos no desenvolvimento da PTDM em protocolos animais. Assim os objetivos do estudo foram avaliar os efeitos da terapia com FK506 por longos períodos sobre os tecidos gengivais, metabolismo do osso alveolar e do cemento de ratos, bem como os efeitos sobre os níveis glicêmicos. Com relação ao metabolismo ósseo, houve uma tendência de diminuição, estatisticamente significante, dos níveis de ALP em todos os períodos tratados com FK506, sendo que com os níveis de cálcio apenas um aumento em 240 dias de tratamento, quando comparados com os grupos controles. Assim, nós podemos concluir que o FK506 em longos períodos pode levar os efeitos deletérios sobre os tecidos gengivais e pode ser dependente da idade, mas não causaria efeitos negativos sobre o osso alveolar e nem ao cemento. E ainda em relação aos níveis séricos de glicemia, os efeitos negativos da terapia com FK506 também são dependentes do tempo. / Abstract: Tacrolimus (FK506) is an immunosuppressive drug in organ transplantation and it is also an alternative therapy used for transplant patients with either refractory graft rejection or intolerant to cyclosporine A (CsA), without the adverse effects frequently attributed to CsA therapy. Nevertheless there are not studies exploring the effects of long-term FK506 therapy on periodontium. It has been suggested that immunosuppressive therapy, mainly FK506, may be an important factor in the development of post-transplantation diabetes mellitus (PTDM). There is a lack of studies exploring the effects of a long-term FK506 on PTDM in animal's protocols. The objectives of the present study were to evaluate the effects of long-term therapy with FK506 on the gingival tissue, alveolar bone metabolism and cementum in rats and the deleterious glycemic effects of long-term therapy with FK506. There was a tendency of increase of serum glycemia level in the initial periods (60 and 120 days). Within the limits of this experimental study, it can be concluded that the deleterious gingival effects of FK506 administration may be time and age related side effects and that have not the negative effects of FK506 administration long-term on the alveolar bone and cementum. It can be concluded that the deleterious glycemia effects of FK506 therapy may be time related side effects. / Doutor

Foi desenvolvido projeto de pesquisa clínica e radiográfica para avaliar a influência do ângulo formado entre a superfície dental e a crista óssea na determinação da profundidade de sondagem do sulco gengival e complementarmente no comportamento da margem gengival por vestibular de dentes molares inferiores inclinados para mesial. Para tanto foram incluídos 30 sítios mesiais e 30 distais no grupo teste, com igual número de sítios controles de molares inferiores com inclinação normal. Nos dentes que forneceram esses sítios também foi feita a determinação da profundidade do sulco gengival na região vestibular central do dente, identificando-se a qualidade e quantidade de gengiva ceratinizada, respectivamente pela metodologia de Kan et al. (2010) e pela mensuração com sonda periodontal da distância da margem gengival à junção mucogengival. Foram incluídos pacientes periodontal e sistemicamente saudáveis, excluindo-se pacientes que tivessem sido submetidos a procedimentos ósseos regenerativos prévios nas áreas de interesse, diabéticos relutantes ao controle médico, usuários de drogas e/ou álcool, portadores de alterações sistêmicas que interfiram no metabolismo ósseo (como por exemplo, osteoporose e hiperparatireoidismo). Os exames foram realizados por examinador competente, devidamente calibrado. Para análise radiográfica as imagens foram transferidas para o computador, realizando-se as mensurações dos ângulos interessados com o programa de computador MB-Ruler Pro (MB-Software solutions). A análise estatística foi realizada no programa GraphPad Prism versão 5.03 para Windows (GraphPad, Usa). Os resultados obtidos em linhas gerais mostraram que houve influência significativa do ângulo entre a superfície dental e a crista óssea (p > 0,0001) na determinação do sulco gengival proximal em áreas de dentes inclinados, porém não há essa influência na profundidade de sondagem do sulco gengival por vestibular para áreas com qualidade e quantidade de gengiva comparáveis ao controle (p=0,08). A despeito desses resultados, não se encontrou correlação definida entre nenhum dos parâmetros de interesse analisados. Dentro dos limites do estudo os resultados também evidenciaram que, embora possam ocorrer variações desses parâmetros, a saúde periodontal pode ser mantida pelo indivíduo nas condições analisadas. / A clinical and radiographic research project was developed to assess the influence of the angle formed between the tooth surface and the alveolar bone crest in determining the probing depth of the gingival sulcus and complementary also to evaluate the behavior of the buccal gingival margin of mesially inclined molars. Therefore, 30 mesial and 30 distal sites of inclined lower molars were included in the test group, with an equal number of sites of lower molars with normal inclination in the control group. In addition in all the teeth of both test and control groups the depth of the clinical gingival sulcus at the central buccal region of the tooth was assessed by measuring with a periodontal probe, identifying the quality of the keratinized gingiva through the methodology of Kan et al. (2010) and determining the width of keratinized gingiva by measuring the distance from the free gingival margin to the mucogingival junction. The study was done in systemic and periodontally healthy individuals, excluding patients who had undergone bone-regenerative procedures in the areas of interest, diabetics reluctant to medical control, alcohol and / or drug users, and individuals suffering from systemic conditions that might interfere with bone metabolism (like osteoporosis and hyperparathyroidism). The examinations were performed by a competent and properly calibrated examiner. The radiographic images were then transferred to a computer in order to analyze the measurements of the involved angles with the computer program MB-Ruler Pro (MB-Software solutions). Statistical analysis was performed using GraphPad Prism version 5.03 for Windows (GraphPad, Usa). The results in general showed that there was significant influence of the angle between the tooth surface and the alveolar bone crest on the depth of the gingival sulcus in proximal areas of inclined teeth (p > 0,0001), but there was no such influence in the probing depth of the buccal gingival sulcus of these inclined molars provided that the quality and quantity of gingiva were similar to controls comprised by molars with normal inclination (p=0,08). Despite this significance, there was no definite correlation between any of the analyzed parameters of interest. The results also showed that, although there may be variations of these parameters, within the limits of this study the conditions of periodontal health can be maintained by the individual.

Objetivo: Na maxila anterior em indivíduos com fissuras labiopalatinas, estabelecer medidas quantitativas dos tecidos moles (qualidade e quantidade da mucosa ceratinizada e medida do vestíbulo bucal) antes e após o enxerto ósseo alveolar secundário tardio (EOAS) com e sem a realização de vestíbuloplastia associada ao enxerto gengival autógeno livre (EGL), prévio ao EOAS tardio. Material e Métodos: O grupo A compreendeu 24 indivíduos com fissuras transforame incisivo unilaterais e o grupo B, 14 indivíduos totalizando 20 fissuras (8 com fissuras transforame incisivo unilaterais e 6 bilaterais). Antes da vestibuloplastia e EGL (grupo B), e pré e 2 meses após o EOAS (grupos A e B), foram medidas a espessura e largura da mucosa ceratinizada e a profundidade do vestíbulo bucal. Resultados: Após a vestibuloplastia e EGL, foram obtidas medidas satisfatórias no grupo B para a realização do EOAS, e após o EOAS, ambos os grupos demonstraram redução dessas medidas. Conclusão: A vestibuloplastia e o EGL antes do EOAS tardio produziram melhora em todos os parâmetros clínicos avaliados, sugerindo benefícios de sua realização neste período; entretanto, a decisão clínica, considerando a particularidade anatômica de cada fissura labiopalatina, deve ser o critério primordial para estabelecer a realização ou não destes procedimentos periodontais. / Objective: To establish quantitative measurements of soft tissues (quality and quantity of keratinized mucosa and measurement of buccal vestibule) in the anterior maxilla in individuals with cleft lip and palate before and after late secondary alveolar bone graft (SABG) with or without accomplishment of vestibuloplasty associated with free autogenous gingival graft (FGG), before the late SABG. Material and Methods: Group A was composed of 24 individuals with complete unilateral cleft lip and palate and Group B included 14 individuals adding up to 20 cleft sites (8 with complete unilateral cleft lip and palate and 6 with complete bilateral cleft lip and palate). Before vestibuloplasty and FGG (group B) and before and 2 months after SABG (groups A and B), the thickness and width of keratinized mucosa and depth of buccal vestibule were measured. Results: After vestibuloplasty and FGG satisfactory measurements were obtained for group B, for accomplishment of SABG, and after SABG both groups presented reduction of these measurements. Conclusion: Vestibuloplasty and FGG before late SABG improved all clinical parameters analyzed, suggesting benefits of its accomplishment in this period. However, the clinical decision considering the anatomical particularity of each cleft lip and palate should be the main criterion for indication of these periodontal procedures.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2015-12-09T16:37:05Z No. of bitstreams: 1 jescaneftalinogueirasilva.pdf: 1568191 bytes, checksum: f265e2e8c149f785a2db409c3d85c37c (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2015-12-10T13:58:43Z (GMT) No. of bitstreams: 1 jescaneftalinogueirasilva.pdf: 1568191 bytes, checksum: f265e2e8c149f785a2db409c3d85c37c (MD5) / Made available in DSpace on 2015-12-10T13:58:44Z (GMT). No. of bitstreams: 1 jescaneftalinogueirasilva.pdf: 1568191 bytes, checksum: f265e2e8c149f785a2db409c3d85c37c (MD5) Previous issue date: 2015-04-27 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O objetivo no presente estudo foi avaliar a espessura da tábua óssea vestibular e do tecido gengival na região de incisivos superiores, em imagens de tomografia computadorizada de feixe cônico (TCFC), obtidas com e sem afastador labial. Tratase de um estudo clínico-tomográfico em que foram coletados dados da espessura da tábua óssea e gengiva vestibular de 120 incisivos superiores. Em exames de TCFC, obtidos com e sem afastador labial, foram realizadas medidas de espessuras óssea e gengival em diferentes regiões. Para validar a utilização da TCFC na mensuração do tecido gengival, medidas tomográficas foram correlacionadas com as medidas obtidas por sondagem clínica. As imagens com e sem afastador foram ainda comparadas em relação a sua qualidade. A medida de espessura óssea localizada na porção mais cervical (1 mm acima da crista óssea) apresentou uma média superior para os exames realizados com afastador labial (teste t pareado/ p = 0,021). As medidas de espessura da tábua óssea apresentaram correlações significativas com as espessuras do tecido gengival (correlação de Pearson/ p ≤ 0,02). O tecido gengival pode ser mensurado apenas em exames de TCFC obtidos com afastador labial, e as espessuras gengivais obtidas em TCFC apresentaram correlações significativas com aquelas obtidas clinicamente (correlação de Pearson/ p ≤ 0,001). Em relação à preferência dos avaliadores, 76% dos radiologistas e 56% dos implantodontistas preferiram as imagens obtidas com o afastador. Concluiu-se que a utilização de afastadores labiais durante a obtenção de exames de TCFC é uma técnica simples e confiável, que permitiu, além da avaliação do tecido ósseo, a mensuração dos tecidos gengivais. / The aim of this study was to evaluate the thickness of the buccal bone and the gingival tissue in the region of upper incisors by using computed tomography cone beam (CBCT), obtained with or without lip retractor. This is a clinical-tomographic study, in which the thickness of the buccal bone and gingiva were collected from 120 upper incisors. Bone and gingival thickness, with and without lip retractor, were measured in different regions of CBCT images. To validate the use of CBCT for measuring gingival tissue, the tomographic measurements were correlated with data acquired by clinical probing. The images with or without lip retractor were also compared regarding their quality. The bone thickness measurement located at the cervical portion (1 mm above the bone crest), was higher when lip retractor was employed (paired t-test/ p = 0.021). The thicknesses of the bone tissue were significantly correlated with those of the gingival tissue (Pearson correlation/ p ≤ 0.02). The gingival tissue could only be measured in CBCT scans obtained with lip retractor. The gingival thickness from CBCT showed significant correlations with those obtained clinically (Pearson correlation/ p ≤ 0.001). Regarding the preference of the evaluators, 76% of radiologists and 56% of implant specialists preferred the images obtained with the lip retractor. It was concluded that in addition to the classical bone tissue examination, the use CBCT with lip retractor is a simple and reliable technique for measuring the gingival tissues.

Hintergrund. Die zahnmedizinische Versorgung und Oralhygiene bei Menschen mit Demenz (MmD) ist auch unter Berücksichtigung der zunehmenden Anzahl der erhaltenen Zähne schwierig. Ziel der Arbeit. Es soll geklärt werden,ob das Angebot einer Beratungsstelle zum Thema Mundgesundheit und Demenz genutzt wird. Material und Methoden. Die Beratungsstelle wurde mit 3 zahnmedizinischen Spezialisten aufgebaut, und ein Jahr lang wurden die telefonischen Anfragen gesammelt sowie ausgewertet. Ergebnisse. Die zahnmedizinische Beratung wurde von 355 Anrufern (180 medizinisch-pflegerisch tätig; 175 Angehörige) im Zeitraum vom 01.06.2012 bis 31.05.2013 genutzt. Von den Anrufern wurden in den ca. 21 min dauernden Gesprächen 2535 Probleme angesprochen. Fast ein Viertel der Anrufer fragte nach einer mobilen Behandlung, und 14,4% suchten einen Zahnarzt, der auf die Behandlung der MmD spezialisiert ist. Orale Erkrankungen, die Mundhygiene und die interpersonellen Schwierigkeiten, die zwischen allen Beteiligten zur oralen Situation auftraten, wurden thematisiert. Drei Viertel des medizinisch-pflegerischen Personals fragten nach Schulungen, um die Mundgesundheit und Oralhygiene bei MmD zu verbessern. Schlussfolgerung. Die Nachfrage bei den Spezialisten zeigte, dass es großen Wissensbedarf sowohl beim Fachpersonal als auch bei den Angehörigen gibt. Die Studie belegt, dass die Probleme in der Mundhöhle von MmD breit gefächert sind. Wissen zu Mund- und Prothesenhygiene sowie den Krankheitsbildern der Mundhöhle könnte strukturiert in die Aus- und Fortbildung eingebracht werden. Neben dem Wissensdefizit wird auch deutlich,dass die zahnmedizinische Versorgungsstruktur nicht für die MmD gesichert ist. Hier sind die Politiker und die Akteure im Gesundheitswesen aufgefordert, die zahnmedizinische Versorgung in den Demenznetzwerken und -strategien zu verankern.:Bibliographische Beschreibung..................................................................4 Einführung..............................................................................................5 Krankheitsbild Demenz.............................................................................5 Einteilung der Formen der Demenz...........................................................8 Verlauf einer Erkrankung an Demenz......................................................10 Ursachen und Symptome der Demenz....................................................12 Zur Notwendigkeit der Behandlung von Menschen mit Demenz...............13 Bisheriger Kenntnisstand zur Versorgungsstruktur von Menschen mit Demenz.................................................................................................14 Zum Nutzen der vorliegenden Studie......................................................15 Publikation.............................................................................................16 Zusammenfassung.................................................................................23 Einleitung..............................................................................................23 Ziel der Studie........................................................................................24 Material und Methode.............................................................................25 Ethische Überlegungen...........................................................................25 Ergebnisse der Studie.............................................................................26 Diskussion.............................................................................................27 Literaturverzeichnis................................................................................29 Anlagen.................................................................................................34 Erklärung über die eigenständige Abfassung der Arbeit............................34 Publikation.............................................................................................36 Danksagung...........................................................................................38 / Background. The complexity of dental care and oral hygiene for people with dementia increases with increasing numbers of residual teeth. Aim. This study was carried out to determine whether the offer of a telephone helpline on oral health and dementia would be utilized. Material and methods. Over a period of 1 year contacts received by a telephone helpline manned by three oral healthcare staff were collected and analyzed. Results. From 01 June 2012 to 31 May 2013 a total of 355 callers (180 healthcare staff and 175 relatives) presented and discussed 2535 problems. The average duration of a call was 21 min. Almost one quarter of the callers were looking for mobile dental care services and 14,4% were looking for a dentist specializedin caring for patients with dementia. Oral disease, oral care and conflicts between stakeholders on aspects of oral health were discussed. Of the healthcare staff three quarters asked about training to improve the oral health of people with dementia. Conclusion. The demand on the specialists of the helpline showed a substantial knowledge deficit of healthcare staff and relatives. The study confirmed a wide range of oral health issues of people with dementia. Knowledge on oral and denture hygiene and on oral disease could be introduced into primary training and continued professional education. In addition to the lack of knowledge, deficits also exist in the oral healthcare system for people with dementia. Poliicians and healthcare stakeholders are encouraged to incorporate oral healthcare for people with dementia into strategies and dementia networks.:Bibliographische Beschreibung..................................................................4 Einführung..............................................................................................5 Krankheitsbild Demenz.............................................................................5 Einteilung der Formen der Demenz...........................................................8 Verlauf einer Erkrankung an Demenz......................................................10 Ursachen und Symptome der Demenz....................................................12 Zur Notwendigkeit der Behandlung von Menschen mit Demenz...............13 Bisheriger Kenntnisstand zur Versorgungsstruktur von Menschen mit Demenz.................................................................................................14 Zum Nutzen der vorliegenden Studie......................................................15 Publikation.............................................................................................16 Zusammenfassung.................................................................................23 Einleitung..............................................................................................23 Ziel der Studie........................................................................................24 Material und Methode.............................................................................25 Ethische Überlegungen...........................................................................25 Ergebnisse der Studie.............................................................................26 Diskussion.............................................................................................27 Literaturverzeichnis................................................................................29 Anlagen.................................................................................................34 Erklärung über die eigenständige Abfassung der Arbeit............................34 Publikation.............................................................................................36 Danksagung...........................................................................................38

No description available.

O enxerto gengival livre (EGL) promove aumento do tecido queratinizado, mas apresenta contração tecidual, problemas estéticos e dor pós-operatória. Recentemente, uma técnica modificada de EGL visa a menor contração e melhor coloração. O objetivo deste estudo foi comparar a eficácia da técnica modificada de EGL com a técnica original de EGL, em relação à largura ápico-cervical do tecido queratinizado após 1 ano de seguimento. Para isso, foi realizado um ensaio clínico randomizado, multicêntrico, em que 40 indivíduos foram submetidos a uma cirurgia de aumento de tecido queratinizado na região de incisivos inferiores com a técnica original (Grupo controle; n=20) ou com a técnica modificada (Grupo teste; n=20). O preparo do leito receptor deu-se de forma idêntica em ambas as técnicas. No grupo controle, o EGL foi estabilizado com suturas e deixado exposto. No grupo teste, o EGL foi recoberto pelo retalho. O desfecho primário foi a largura ápico-cervical do tecido queratinizado (LTQ) e, juntamente com a espessura do tecido queratinizado (ETQ) e os parâmetros clínicos: retração gengival (RG), profundidade clínica de sondagem (PS), nível clínico de inserção (NCI), índice PASS (IPASS) e índice de sangramento à sondagem (ISS), foram analisados antes da cirurgia e aos 3, 6 e 12 meses após a cirurgia. O tempo transcirúrgico (TT), a dor pós-operatória (DPO) na área doadora e receptora, a quantidade de medicação consumida (QM), a contração vertical do enxerto (CV), a correspondência de cor da gengiva (COR) e a satisfação estética do paciente (SE), também foram avaliadas. O teste t e ANOVA de medidas repetidas, seguido do teste post-hoc de Newman-Keuls foram utilizados para análise dos desfechos. O nível de significância foi estabelecido em 5% (p<0,05). Ambas as técnicas promoveram aumento de LTQ e ETQ. Não houve diferença significativa entre as técnicas para LTQ, ETQ, CV, COR e SE. O grupo teste apresentou significativamente menos dor, tanto na área doadora quanto na receptora, aos 7 dias. Ademais, consumiu significativamente menos analgésicos durante o período pós- operatório. Portanto, a técnica modificada de EGL foi tão eficaz quanto a técnica original no aumento da largura ápico-cervical de tecido queratinizado, apresentando como vantagem um menor desconforto pós-operatório. / Free gingival graft (FGG) promotes keratinized tissue augmentation, though graft shrinkage, esthetic issues and postoperative pain may occur. Recently, a modified technique was proposed aiming less shrinkage and better color matching. The objective of this study was to compare the efficacy of the modified FGG technique with the original technique, in relation to the apico-coronal width of keratinized tissue after 1 year of follow-up. A multicentric randomized clinical trial was designed and included 40 subjects who were submitted to a surgery for keratinized tissue augmentation in lower incisor area. Control group (n=20) received the original technique and test group (n=20) received the modified technique. Recipient area was prepared identically for both groups. In control group, FGG was sutured and left exposed whereas in test group, FGG was recovered by the flap. Primary outcome was the apico-coronal width of keratinized tissue (WKT). Additionally, thickness of keratinized tissue (TKT) and clinical parameters: gingival recession (GR), probing depth (PD), clinical attachment level (CAL), PASS index (PI) and bleeding on probing (BoP) were measured before surgery and after 3, 6 and 12 months. Surgery time (ST), postoperative pain (POP) at recipient and donor site, number of pain killers consumed (PC), vertical shrinkage (VS), color matching (CM) and patient\'s esthetic satisfaction (ES) were also analyzed. Statistical analysis of the outcomes was performed with t test and ANOVA for repeated measures, followed by the Newman-Keuls post-hoc test. Significance level was stablished at 5% (p<0.05). Both techniques were effective in augmenting WKT and TKT. There was no statistically difference between techniques for WKT, TKT, VS, CM and ES. Test group showed less pain at recipient and donor site at 7 days of follow-up. Also, test group consumed significant less pain-killers. Therefore, the modified technique of FGG was as effective as original technique in augmenting apico-coronal width of keratinized tissue, but presented less postoperative discomfort as an advantage.

La thérapie cellulaire est une méthode d’avenir innovante, actuellement utilisée dans le traitement de pathologies multiples (auto-immunitaire, cancéreuses, pathologies inflammatoires, allogreffes…) et la régénération des pertes de substance tissulaire. Les cellules souches mésenchymateuses, par la variabilité de leurs origines, présentent des propriétés très intéressantes à la thérapie, notamment un potentiel de différenciation en lignées multiples, et des propriétés d’immunomodulation importantes. Mon projet s’intéresse à l’utilisation de cellules souches orales récemment isolées de la gencive par notre équipe : cellules souches gingivales (GSC), et présentant un avantage fonctionnel par rapport aux sources cellulaires traditionnelles d’origine mésodermique (moelle osseuse) ou orales (pulpe dentaire, follicule dentaire, ligament parodontal, glandes salivaires…). Les défauts osseux des mâchoires, de par leur multitude d’étiologies (traumatismes, dysmorphoses, cancer, ...) et le handicap généré, représentent une cible thérapeutique privilégiée. Les GSCs ont la même origine embryologique neurectodermique que les os maxillaires et par là-même un phénotype proche, exploré dans notre équipe. Cette source gingivale de prélèvement non traumatique est une alternative aux techniques chirurgicales actuelles mutilantes pour le site donneur. Notre objectif est double : Etablir un protocole préclinique de culture des GSC en ostéoblastes, pour être compatibles avec la thérapie humaine afin d’obtenir une régénération osseuse optimale. Les capacités immunomodulatrices des GSCs sont par là-même étudiées dans ces nouvelles conditions, dans le but de maitriser la réaction inflammatoire et préserver la greffe osseuse, grâce à la plateforme exceptionnelle mise à notre disposition par l’établissement français du sang, et une équipe très spécialisée dans l’étude des mécanismes de régulation immunitaires. Nos résultats permettront non seulement une régénération osseuse transposable chez l’homme, mais également d’utiliser ces cellules pour le traitement d’autres pathologies (cancéreuses, auto-immunitaires…) en utilisant leur capacité immunomodulatrice. / Cell therapy is an innovative method of the future, currently used in the treatment of multiple diseases (autoimmune, cancer, inflammatory pathologies, allografts ...) and the regeneration of tissue loss. Mesenchymal stem cells (MSC), regardless their origins, exhibit very interesting properties for therapy, including a potential for multi-line differentiation, and important immunomodulation properties. My project focuses on the use of oral stem cells recently isolated from the gingiva by our team (GSC), and having a functional advantage over traditional mesodermal (bone marrow) cellular sources. The bone defects of the jaws, due to their multitude of etiologies (trauma, dysmorphoses, cancer...) and the generated handicap, represent a preferred therapeutic target. GSCs have the same neurectodermal embryological origin as the maxillary bones and thus a similar phenotype, explored in our team. This gingival source of non-traumatic removal is an alternative to current mutilating surgical techniques for the donor site. Our goal is twofold: To establish a preclinical GSC culture protocol in osteoblasts, to be compatible with human therapy, in order to achieve optimal bone regeneration. The immunomodulatory capacities of the GSCs are themselves studied under these new conditions, with the aim of controlling the inflammatory reaction and preserving the bone graft, thanks to the exceptional platform made available to us by the French blood establishment, and A highly specialized team in the study of immune regulation mechanisms. Our results will not only allow transposable bone regeneration in humans but also use these cells for the treatment of other pathologies (cancerous, autoimmune ...) using their immunomodulatory capacity.