Global ETD Search

61	Immunhistochemischer Algorithmus zur Diagnostik maligner Keimzelltumoren des Hodens / Immunohistochemical algorithm for the diagnosis of malignant germ cell tumors of the testis Mayer-Eichberger, Katharina 28 March 2011 (has links) No description available. 610 Medizin, Gesundheit Medicine Keimzelltumoren Hoden Tissue-Microarray Immunhistochemie PLAP Oct-3/4 D2-40 CD117 CD9 CD30 Pan-CK Sox2 AFP ß-HCG Seminom Nichtseminom IGCNU germ cell tumors testis tissue-microarray immunohistochemistry PLAP Oct-3/4 D2-40 CD117 CD9 CD30 Pan-CK Sox2 AFP ß-HCG seminoma nonseminoma IGCNU 44.47 MED 391: Pathologie {Medizin}
62	Analysis Of Structural And Functional Types Of Protein-Protein Interactions Nambudiry Rekha, * 02 1900 (has links) (PDF) No description available. Protein-Protein Interactions Protein Structures Protein Interfaces Human Chorionic Gonadotropin (hCG) Protein Kinases RNA Polymerase II Protein Complexes Antibodies Epitope Sites Protein Kinase CK2 Tethered Protein Domain Interacting Proteins Biochemistry
63	Étude de l’expression et de la fonction du gène Ankyrin-repeat and SOCS-Box protein 9 (ASB9) dans le follicule ovulatoire bovin Benoit, Gabriel 04 1900 (has links) No description available. ASB9 Ovaire Cellules de granulosa Follicule ovulatoire Ovulation LH hCG Vache laitière Fertilité Double hybride CRISPR-Cas9 Ovary Granulosa cells Ovulatory follicle Dairy cow Fertility Yeast two-hybrid
64	Αξιολόγηση των επιπέδων της AFP και β-hCG στον ορό της εγκύου κατά το β' τρίμηνο τησς κύησης με σκοπό την πρόβλεψη δυσμενούς έκβασης της κύησης Γκόγκος, Παναγιώτης 27 April 2009 (has links) Σκοπός της παρούσας προοπτικής µελέτης είναι η διερεύνηση της σχέσης µεταξύ των επιπέδων της α-φετοπρωτείνης και της β-hCG στον ορό της µητέρας κατά το β' τρίµηνο της κύησης και της δυσµενούς έκβασης της κύησης σε τυχαίο δείγµα εγκύων γυναικών της Νοτιο-Δυτικής Ελλάδος. Υλικό - Μέθοδος: 130 υγιείς γυναίκες ελληνικής καταγωγής µε αυτόµατη έναρξη της εγκυµοσύνης µελετήθηκαν για τα επίπεδα της AFP και της β-hCG στον ορό τους µεταξύ της 13ης-24ης εβδοµάδας της κύησης και παρακολουθήθηκαν για δυσµενή έκβαση της εγκυµοσύνης. Επίπεδα AFP καθώς και επίπεδα β-hCG στον ορό της εγκύου 2 φορές µεγαλύτερα του µέσου όρου για την ηλικία της κύησης θεωρήθηκαν µη φυσιολογικά. Η στατιστική ανάλυση έγινε µε τη δοκιµασία Pearson's x2. Αποτέλεσµα: Αυξηµένα επίπεδα AFP στον ορό της µητέρας βρέθηκαν σε 27 από τις 130 γυναίκες που µελετήθηκαν (20,7%). Μεταξύ αυτών µόνο 4 γυναίκες (14,8%) ανέπτυξαν επιπλοκές της κύησης. Επίσης αυξηµένα επίπεδα β-hCG βρέθηκαν σε 14 από τις 130 γυναίκες που µελετήθηκαν (10,77%). Όµως µεταξύ αυτών καµία γυναίκα δεν ανέπτυξε επιπλοκές κατά τη διάρκεια της παρούσας κύησης. Συµπέρασµα: Ένας πολυπαραγοντικός έλεγχος της λειτουργίας του πλακούντα κατά το β' τρίµηνο της εγκυµοσύνης µε Doppler των µητριαίων αρτηριών, µε έλεγχο της µορφολογίας του πλακούντα, µε έλεγχο των επιπέδων της AFP και της β-hCG στον ορό της εγκύου δυνατό να µας επιτρέψει την αναγνώριση εκείνων των εγκύων µε αυξηµένο κίνδυνο να αναπτύξουν σοβαρή πλακουντιακή ανεπάρκεια και επιπλοκές της κύησης. / In our prospective study, we investigated the association between midtrimester maternal serum AFP (ms-AFP) and midtrimester maternal serum β-hCG (ms-β- hCG) levels and adverse pregnancy outcome, in the South-Western Greek population. Materials and Methods: 130 healthy Greek women with spontaneous pregnancies, investigated for ms-AFP levels between 13th-24th weeks of gestation and followed for adverse pregnancy outcome. AFP levels > 2,0 multiples αnd β-hCG levels>2,0 multiples of the median value for gestation, were considered abnormal. Statistical analysis was performed by Pearson's chi-square test. Results: Elevated ms-AFP levels were detected in a total of 27 from the 130 women studied (20,7%). Among them, only 4 women (14,8%) developed pregnancy complications. Elevated ms-b-hCG levels were detected in total of 14 from the 130 women studied (10,77%). Νοne of them developed pregnancy complications. Conclusion: Multiparameter testing of placental function in the mid-trimester uterine artery Doppler, placental morphology, ms-AFP and ms-hCG screening may allow us to identify women with increased risk to develop severe placental insufficiency and pregnancy complications. Β' τρίμηνο της κύησης 618.207 5 Maternal serum AFP levels Maternal serum β-hCG levels Adverse pregnancy outcome Mid-trimester
65	Engineering the N-Glycosylation Pathway in Pichia Pastoris for the Expression of Glycoprotein Hormones Manoharan, Simna January 2016 (has links) (PDF) Proteins, participating in a myriad of biological function, are at the core of all cellular activities occurring within living organisms. Therapeutic proteins, hence constitute a major part of the pharmaceutical industry. The glycoprotein hormones follicle stimulating hormone (FSH), luteinizing hormone (LH), thyroid stimulating hormone (TSH) and human chorionic gonadotropin (CG) regulate various reproductive and metabolic functions in humans and hence have high therapeutic potentials. The increasing demand of recombinant proteins for therapeutic uses drives the development of better expression systems. The methylotrophic yeast Pichia pastoris, has been termed as an industrial workhorse for heterologous protein expression. However, the N-glycosylation in yeast is of the high mannose type, resulting in a reduced serum half-life of the recombinant proteins. In the current work, we have re-engineered the Pichia N-glycosylation pathway to mimic the human type of N-glycosylation. Towards this end, we abolished the yeast native N-glycosylation and introduced enzymes from various eukaryotic sources into the system. These modifications resulted in the conversion of the yeast Man9-20GlcNAc2 glycan structure to a more human like GlcNAc2Man3GlcNAc2 form on over 70 % of the heterologous expressed proteins. In order to demonstrate the application of these strains as efficient protein expression hosts, the glycoengineerd Pichia was used for large scale expression of the glycoprotein hormones, hCG and FSH. The purified recombinant hormones were found to have binding affinities and structure similar to that of the natural hormones. These recombinant hormones were also able to elicit over two fold responses in animal models compared to buffer controls and the activity was comparable to the natural counterparts. Thus, we report the generation of a glycoengineered Pichia pastoris, which can be considered as a serious contender for the expression of glycosylated proteins of therapeutic importance. Glycoprotein Hormones N-Glycosylation Recombinant Protein Expression Pichia Pastoris N-Glycosylation Industrial Proteins Glycosylated Protein Expression Protein Glycosylation Glycoengineered Pichia Pastoris Human Chorionic Gonadotropin (hCG) Follicle Stimulating Hormone (FSH) Therapeutic Proteins Glycoprotein Hormone Expression Recombinant Therapeutic Proteins Pichia pastoris Glycoengineered Strains Chemical Engineering

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