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Abstract The Hai-Wai-Chi Society, an overseas organization derived from the ¡§Chi Society¡¨ based in Shanghai, was initiated and founded by Hsu Fu-Yuan, one of the six poets of the Chi Society. Towards the end of the Hung Kung reign of the late Ming dynasty, in the wake of the defeat of the Sungchiang uprising staged by the society, Hsu continued to work for the recovery of the Ming legacy against the ruling Qing regime. At first, he left Shanghai to follow Emperor Lungwu in Fujian. After Emperor Lungwu was defeated, he proceeded to Choushan to follow King Lu and cooperated with Chang Huang-Yen. Later on, after Choushan was seized by the Qing army, Hsu accompanied King Lu under the shelter of Koxinga in Xiamen. It was at this point that the society came to its apex. When Koxinga took hold of Taiwan following his defeat at Nanjing battle, the society members began to enter the island along with the soldiers. Therefore, the literature of Hai-Wai-Chi Society is viewed as the beginning of Han Taiwanese literature. Taiwan Shih Cheng by Lien Heng refers to Hsu Fu-Yuan, Chang Huang-Yen, Lu Jo-Teng, Sheng Chuan-Chi, Chao Tsung-Lung, Chen Shih-Ching as ¡§The Six Poets of Hai-Wai-Chi Society.¡¨ This paper, entitled A Study of Three Poets in the Hai-Wai-Chi Society, is intended to follow the same way of thinking in its exploration of Hsu Fu-Yuan, Chang Huang-Yen, and Lu Jo-Teng through the existing anthology of the society. Meanwhile, the relevant literature concerning the other three poets will be reviewed for necessary references. The Hai-Wai-Chi Society is characterized by its composition of core members, mainly loyalists to King Lu, who followed Koxinga to Taiwan as a consequence of the fall of Choushan. Mourning over the end of the Ming empire, intellectuals of high morality and talent assembled to form a society of poetry to express their emotions toward the fatherland. The members of the Hai-Wai-Chi Society were actively involved in the fight against the Qing court. In spite of successive defeats, they would live and die in exile overseas rather than surrender as subjects of the Qing regime. The Introduction aims to explain the motivation of this study, the meaning and formation of The Hai-Wai-Chi Society, with a literature review of the three poets. The first chapter explores the public opinions of The Dong-Lin Party¡]ªFªLÄÒ¡^ and the Restoration Society¡]´_ªÀ¡^ in the Late Ming period, and the poetry of patriotism by Chen Tzu-Lung and Hsia Wang-Chun of The Hai-Wai-Chi Society, to be defined in this study as two typical kinds of heritage of The Hai-Wai-Chi Society. The second chapter gives an analysis of the historical background of South Ming royalists fighting against the Qing regime and the formation and development of The Hai-Wai-Chi Society. The third chapter is focused on the Tiao-Huang-Tang Manuscript by Hsu Fu-Yuan to study his overseas poems that express his uncompromising anti-Qing mentality and noble patriotic integrity. The fourth chapter represents the social reality of war-ridden Kinmen during the Koxinga period based on Niao-Yi Poetry and Liu-An Anthology by Lu Jo-Teng. The fifth chapter deals with Chi-Ling Grass, Ping-Tsa Collection, and Tsai-Wei Recitation by Chang Huang-Yen to distinguish him as a great noble Ming loyalist who had fought against the Qing power for 19 years overseas. The Conclusion sums up the poetry by the three poets as: (1) a reflection of history with a national concern, (2) an expression of opposition against invasion with a firm anti-Qing determination, and (3) a kind of oceanic literature with a universal sympathy.

Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable, empowering researchers to elucidate the functional organization of the genome at the systems level and establish causal linkages between genetic variations and biological phenotypes. To facilitate successful and specific Cas9 targeting, we first optimize the guide RNAs (sgRNA) to significantly enhance gene editing efficiency and consistency. We also systematically characterize Cas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target mutagenesis. We find that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches. We also show that Cas9-mediated cleavage is unaffected by DNA methylation and that the dosage of Cas9 and sgRNA can be titrated to minimize off-target modification. Additionally, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. We next demonstrate that Cas9 nickase mutants can be used with paired guide RNAs to introduce targeted double-strand breaks. Because individual nicks in the genome are repaired with high fidelity, simultaneous nicking via appropriately offset guide RNAs can reduce off-target activity by over 1,500-fold in human cells. In collaboration with researchers at the University of Tokyo, we further identified a PAM-interacting domain of the Cas9 nuclease that dictates Cas9 target recognition specificity. Finally, we present protocols that provide experimentally derived guidelines for the selection of target sites, evaluation of cleavage efficiency and analysis of off-target activity. Beginning with target design, gene modifications can be achieved within as little as 1-2 weeks. Taken together, this work enables a variety of genome engineering applications from basic biology to biotechnology and medicine.

No description available.

本論文計分六章，各章章旨及其內容如下： 　　第一章為「緒論」。內容包括：「研究動機與目的」、「研究現況檢討」、「研究方法與取材」及「研究侷限與發現」。本章旨在敘述研究背景，藉由研究現況的檢討，掌握值得開發的研究空間與契機，並進一步揭示論文研究的心得。 　　第二章為「許地山的生平與思想」。探討主題包括：「戰爭對其人格特質、生命情調的啟發」、「家庭對其人道關懷、人生哲學的造就」、「治學對其知識結構、人文素養的塑成」及「時勢對其文化態度、文化參與的影響」。本章旨在透過許地山的生平與思想的交叉論述，以影響研究的觀點，彰顯許地山人格與思想的成因，並透視其人富於時代意識的特色。 　　第三章為「許地山的文藝觀」。討論範圍涵蓋：「許地山的文藝創作論」、「許地山的文藝鑒賞論」和「文藝觀的淵源與特色」。本章旨在爬梳並歸納許地山的文藝思想，分別從創作論及鑒賞論兩部分，分述其具體內容，再綰合兩者，尋繹許地山文藝觀的淵源與特色。藉以掌握許地山創作傾向的理論根據，並作為進一步解讀許地山文本的準備。 　　第四章為「許地山作品的主題意蘊」。章分四節分別探討許地山文學作品所呈現的「生命觀照與人生態度的闡發」、「情愛觀與兩性觀的宣達」、「社會現實的揭露和批判」及「去國與懷鄉──臺灣風味的書寫」。本章旨在透析許地山文學作品的主題意蘊，再回扣到許地山的生平行誼與思想內涵，驗證許地山其文實為其人之全人格的表現。 　　第五章為「許地山文學的藝術表現」。就許地山文學的藝術表現，舉其凸顯的成就作一闡釋與驗證，內容包括：「許地山文學的寓言特質」、「許地山散文的賦體成分」、「許地山文學的抒情傾向」及「女性人物的理想性格與形象」。本章旨在全面掌握許地山文學的藝術特色與成就。從許地山文本的微視與剖析後，得其主要特色有三，亦即寓言特質、賦體成分與抒情傾向。再從許地山小說人物的考察，檢視其形象塑造的得失，透過歸納許地山人物性格與形象的特徵，逼顯許地山理想的人物性格及形象。 　　第六章為「結論：許地出文學的意義與定位」。本章旨在總結本論文的研究心得。於許地山其人其文有了全面而深入的了解後，將許地山的文學回置到我國文學發展的歷史中，考察許地山文學的意義與歷史定位，獲致許地山文學的兩項意義，即許地山乃現代散文的奠基者（寓言散文的先驅）、五四文壇的臺灣代言人。 《許地山文學之研究》 論 文 目 錄 第一章 緒論 第二章 許地山的生平與思想 第一節 戰爭對其人格特質、生命情調的啟發 第二節 家庭對其人道關懷、人生哲學的造就 第三節 治學對其知識結構、人文素養的塑成 第四節 時勢對其文化態度、文化參與的影響 結 語 第三章 許地山的文藝觀 第一節 許地山的文藝創作論 第二節 許地山的文藝鑒賞論 第三節 文藝觀的淵源與特色 結 語 第四章 許地山文學的主題意蘊 第一節 生命觀照與人生態度的闡發 第二節 情愛觀與兩性觀的宣達 第三節 社會現實的揭露和批判 第四節 去國與懷鄉──臺灣風味的書寫 結 語 第五章 許地山文學的藝術表現 第一節 許地山文學的寓言特質 第二節 許地山散文的賦體成分 第三節 許地山文學的抒情傾向 第四節 女性人物的理想性格與形象 結 語 第六章 結論：許地山文學的意義與定位 參考書目 附錄：許地山生平暨作品繫年 國立政治大學中國文學系碩士論文 指導教授：簡宗梧博士

許學夷，字伯清，南直隸江陰（今江蘇江陰）人，生於明嘉靖四十二年（1563），卒於崇禎六年（1633）。其以復古派的立場，歷四十年光陰所著成之《詩源辯體》，是一部體大思精的論詩著作，惜因此書傳世甚稀，《四庫全書》又未予著錄，使得這部詩歌批評史上的罕見的鉅製沈寂了三百多年，直到近幾年方才逐漸受到重視。實則《詩源辯體》是明代復古派反擊公安、竟陵二派的總結性論著，其系統之井然，篇幅之龐大，都是宋代以來的詩話傳統所未見的，且是書詳論《詩經》以降的中國歷代詩歌之源流發展，並兼評歷代詩論和詩選集，實質上即是一部完整的詩歌史和簡易的詩歌批評史，其所具有的價值和意義都不容我們忽視。本文以許學夷的《詩源辯體》為研究對象，論文內容除了〈引言〉之外，總計分成七章：第一章〈許學夷其人其事〉與第二章〈詩源辯體的成書〉，先針對許學夷的生平經歷和《詩源辯體》的成書狀況作一番基本的說明，這兩章是屬於外緣問題的探討；第三章至第六章分別為〈詩史觀──詩歌演變歷史的詮釋〉、〈辨體論──詩歌體裁與家數的辨析〉、〈創作論──復古的創作理論與實踐〉以及〈批評研究──理論、實際與批評史〉，這四章則是依據《詩源辯體》的內容和性質，來研究其核心的理論；第七章〈結論〉，則從許學夷詩論的承先啟後以及《詩源辯體》一書的的價值、研究展望等幾個方面，為全文提出最後的總結。《詩源辯體》原本即是系統嚴謹的論著，本文透過個人的詮釋方式，為其書建構另種面貌的理論體系，希望這樣的研究能夠闡發許學夷詩論的精髓，並還給其書一個合理的文學史地位。

University of Macau / Faculty of Social Sciences and Humanities / Department of Chinese

Title from PDF of title page. / Document formatted into pages; contains 84 pages. / Thesis (M.S.)--University of South Florida, 2003. / Includes bibliographical references. / Text (Electronic thesis) in PDF format. / ABSTRACT: Hurricane hazards result from the combined processes of wind, waves, storm surge, and overwash (Lennon et al., 1996). Predicting the severity of these hazards requires immense effort to quantify the processes and then predict how different coastal regions respond to them. A somewhat simpler, but no less daunting task is to begin to predict the hazards due to potential erosion of barrier islands. A four-part scale has been developed by Sallenger (2000) to provide a framework for understanding how barrier islands might respond during extreme storm events. These four regimes describe how beach and dune elevations interact with surge and wave runup. This study will produce estimates of potential hazards through combining lidar surveys of dune elevation with modeled elevations of storm water levels. Direct measurements of maximum wave heights during hurricanes are rare. / ABSTRACT: We evaluated three simple equations proposed by Kjerfve (1986), Young (1988), and Hsu (1998) to forecast the maximum wave height (Hmax) generated by three 1999 hurricanes. Model results were compared to wave data recorded by the National Oceanic and Atmospheric Administration (NOAA) wave rider buoys. The radius of maximum winds, wind speed, forward velocity, distance from buoy to the storm's eye-wall (r), and buoy's position relative to the quadrant of the storm (Q) were found to have significant and direct roles in evaluating recorded hurricane induced wave heights (H) and thus, were individually examined for each comparison. The implications of the r and Q on H were assessed when determining the overall effectiveness of the modelers' equations. Linear regression analyses tested the accuracy of each modeled prediction of the Hmax, comparing it to the observed wave heights. Three statistical criteria were used to quantify model performance. / ABSTRACT: Hsu's model was the most reliable and useful forecasting technique. Despite the predictive skill of Hsu's model, direct observations of the maximum wave conditions, when available and appropriate, are preferred as inputs for SWAN, a 3rd generation shoaling wave model. Outputs from SWAN are used to calculate the empirical relationships for wave runup. For our test case, pre and post-storm topographies were surveyed as part of a joint USGS-NASA program using lidar technology. These data sets were used to calculate changes in the elevation and location of the dune crest (Dhigh) and dune base (Dlow) for the North Carolina Outer Banks. We hindcast potential coastal hazards (erosional hot spots) using the pre-storm morphology and modeled wave runup and compare those estimates to the measured results from the post-storm survey. / ABSTRACT: Links among the existing topography and spatial variations in wave runup were found to be 95% correlated for the north-south and east-west facing barrier islands. Application of Sallenger's (2000) four-part Storm Impact Scale to the pre-storm Dhigh elevation survey and wave runup extremes (Rhigh and Rlow) were found to accurately predict zones of overwash and showed potential to forecast the inundation regime. / System requirements: World Wide Web browser and PDF reader. / Mode of access: World Wide Web.

Dr. Kwan Hsu was neither a superstar nor a celebrity. Her name does not come up in conversations about important contributors to her field of biophysics nor is she instantly recognizable for her contributions to Portland State University's international program or the state of Oregon's business ties with China. Yet she was a contributor, a cog-in-the-wheel, at the very least, in all of these areas and more. She was a peripheral member of a well-known Chinese family, but few in the United States know of or perhaps have interest in, but otherwise, she had no great connections or family ties to generate interest in her story. How does one process a collection for a woman who does not meet the traditional criteria for excellence or success or public interest for an archive? Where is the value to the larger historical narrative of our time in preserving the memories of someone who was non-remarkable, or, conversely, someone who may be even too unique to contribute to that greater narrative? These are the questions I wrestled with when I first came to this collection. As my research progressed, I realized that I faced more questions, and that to come to any understanding that might answer them, I was going to have to research the history of archives and archival processes. Science, the Cold War, Communist China, women, the immigrant experience, all of these issues became part of my thesis, however shallowly I was able to investigate them. Questions of identity and historiography, of power and discourse were explored. In the end, what I found was that a collection that on the outside looked unimpressive and unenlightening, could indeed be very valuable, and provide insight into any number of areas of current interest in historical research. This is that story.

University of Macau / Faculty of Social Sciences and Humanities / Department of Chinese