Introdução: O Protocol Aids Clinical Trial Group (PACTG 076), publicado em 1994, demonstrou que a utilização da zidovudina (ZDV) reduzia a taxa de transmissão materno-infantil do HIV (TMI HIV) de 25% para 8,3%. Atualmente a terapia antirretroviral (TARV) combinada associada a uma série de medidas pode reduzir a taxa de TMI HIV para menos de 2%. Embora o Ministério da Saúde preconize a adoção destas medidas, a TMI HIV ainda permanece acima dos níveis desejados, principalmente em nosso meio. De acordo com levantamento da Vigilância Epidemiológica do Município de Porto Alegre, no ano de 2012 a taxa TMI HIV foi de 2,9%. Objetivo: Avaliar os fatores que interferem na TMI HIV em um Hospital Universitário do Município de Porto Alegre/RS. Método: Trata-se de um estudo de coorte histórico, tendo como amostra 292 bebês nascidos de mulheres portadoras do HIV, cujos nascimentos ocorreram no Centro Obstétrico de um Hospital Universitário do Município de Porto Alegre/RS, entre 1º de janeiro de 2010 a 31 de dezembro de 2014. Resultados: Dos 292 bebês, cujas mães eram portadoras do HIV, 3,8% (n=11) foram contaminados. Destes 90,9% (n=10) nasceram por cesariana; 90,9% (n=10) tinham d37 semanas; 54,6% (n=6) receberam ZDV xarope isoladamente e 45,4% (n=5) receberam ZDV + nevirapina (NVP). Quatro gestantes cujos bebês foram contaminados apresentaram sífilis na gestação (36,4%). A má adesão a TARV (p<0,003), a carga viral d1.000 cópias/mL ou ignorada no 3º trimestre (p<0,001) e o CD4 <500 células/mm3 (p<0,046) no terceiro trimestre foram significativamente associados a maior TMI HIV. Conclusão: Os fatores associados significativamente a TMI HIV foram à má adesão a TARV, a presença de sífilis na gestação, a carga viral d1000 cópias e o CD4 <500 células/mm³ no terceiro trimestre. / Introduction: Protocol Aids Clinical Trial Group (PACTG 076), published in 1994, demonstrated that the use of zidovudine (ZDV) had reduced the rate of mother-tochild transmission (MTCT) of HIV from 25% to 8.3%. Currently, a combined antiretroviral therapy (HAART) associated with a number of measures can reduce the rate of MTCT to less than 2%. Although the Ministry of Health recommends the adoption of these measures, the MTCT still remains above desired levels, especially in our center. According to a survey of Epidemiological Surveillance of Porto Alegre, in 2012 the rate of MTCT was 2.9%. Objective: To evaluate the factors that interfere with MTCT from HIV-positive women who gave birth in a University Hospital of Porto Alegre/RS. Method: A historical cohort study, with a sample 292 babies from HIV infected mother whose delivery occurred at Obstetric Center of the University Hospital of Porto Alegre/RS, at period of January 2010 till December 2014. Results: Of 292 babies of women HIV positive, 3.8% (n=11) were infected. Of those 90.9% (n=10) was born by cesarean section; 90.9% (n=10) had d37 weeks; 54.6% (n=6) received only ZDV syrup and 45.4% (n=5) received ZDV+nevirapine (NVP). Four pregnant women whose babies were infected, mother had syphilis during pregnancy (36.4%). Poor adherence to HAART (p<0.003), viral load d1000 copies/mL or ignored in the third trimester (p<0.001) and CD4 <500 cells/mm3 (p<0.046) in the third trimester were significantly associated with higher MTCT. Conclusion: We conclude that the presence of syphilis in pregnancy, viral load d1000 copies/mL or ignored in the third trimester, the CD4 <500 cells/mm³ in the third trimester, poor adherence to HAART were significant factors for MTCT.

Comprendre les rétroactions entre les maladies infectieuses, la structure des écosystèmes et le développement économique est nécessaire pour alléger le fardeau des maladies tropicales négligées. Ce groupe d'infections parasitaires, virales, et bactériennes est étroitement associé à des conditions géographiques, environnementales, sanitaires et économiques particulières aux régions tropicales. A travers l'étude de l'ulcère de Buruli, une maladie émergente et négligée associé à une morbidité et handicap très importants dans des régions tropicales, ce travail de thèse s'intéresse aux interactions complexes entre ces différents composants des systèmes épidémiologiques. Combinant un travail de terrain important pour la collecte des données environnementales avec une approche de recherche multidisciplinaire, cette thèse vise à améliorer notre compréhension des différents aspects de l'écologie et de l'épidémiologie de cette maladie infectieuse. Notamment, la dynamique de son agent pathogène, M. ulcerans, est caractérisée pour un large éventail d'écosystèmes et communautés aquatiques au Cameroun, permettant d'identifier les facteurs environnementaux permettant sa propagation. En outre, nous évaluons la transmission de l'agent pathogène de l'environnement à l'homme et l'impact de la maladie sur le développement économique des populations endémiques. Ainsi, ce travail montre comment les dynamiques écologiques, épidémiologiques, environnementales et économiques interagissent de concert, mettant en évidence de façon criante le besoin d'une telle approche interdisciplinaire dans l'étude des maladies tropicales négligées. / Understanding the feedbacks between infectious diseases, ecosystem structure and economic development is necessary to alleviate the burden of Neglected Tropical Diseases. This group of parasitic, viral, and bacterial infections is closely associated with particular geographical and environmental conditions mainly present in the tropics, thriving under conditions of poverty, inefficient sanitation and malnutrition. This PhD thesis works through the case study of Buruli ulcer, an emerging and neglected infectious disease associated with a great morbidity and disability burden in tropical regions. Relying on an extensive environmental field survey and a multidisciplinary research approach, this PhD attempts to gain a better understanding of different aspects of the ecology and epidemiology of Buruli ulcer. Notably, the dynamics of its pathogen, M. ulcerans are characterized for a wide range of freshwater ecosystems and aquatic communities in Cameroon, and the environmental drivers of M. ulcerans presence are investigated. Furthermore, we assess the transmission of the pathogen from the environment to humans and the impact of the disease on the economic development of endemic populations. Thus, this work shows how the interplay between ecological, epidemiological and economic dynamics interact together and calls for an urgent need to apply such inter-disciplinary approach to decrease the burden of neglected tropical diseases.

Made available in DSpace on 2015-07-22T20:49:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-12-31 / A terapia anti-retroviral (TARV) contribui para a diminuição da morbidade e da mortalidade, com conseqüente aumento da sobrevida. No Brasil, há diferenças regionais relativas à dinâmica da epidemia do HIV e ao seu enfrentamento no grupo das gestantes e das crianças com HIV/AIDS. Este estudo verifica o tempo de sobrevida após o diagnóstico de aids em 914 crianças infectadas por transmissão vertical, entre os anos de 1983 e 1998 e acompanhadas até 2002, nas cinco regiões brasileiras. O tempo decorrido do nascimento ao diagnóstico de infecção pelo HIV, ao longo dos anos, apresenta uma diminuição, principalmente nos estados das regiões Sul e Sudeste. Houve melhora significativa da sobrevivência, mais de 75% dos casos ainda estavam vivendo quatro anos após o diagnóstico, no grupo de 1997 e 1998. Esta análise brasileira mostra ser possível para um país em desenvolvimento, estabelecer um sistema efetivo de acesso gratuito e universal à TARV, mesmo com dificuldades regionais para a organização de uma infra-estrutura ideal de saúde, tendo como resultado um aumento significativo na sobrevivência. / Antiretroviral therapy contributes to decreasing morbidity and mortality, and consequently increasing survival. In Brazil, there are regional differences about HIV epidemic dynamics and in confront among pregnant women and children with HIV/AIDS. This study verifies survival time after AIDS diagnosis in 914 children infected by mother-to-child transmission, between 1983 and 1998 and followed until 2002, in the five Brazilian regions. Time between birth and HIV diagnosis, along the years, decreased mainly in the states of South and South-East regions. There was a significant improvement in survival; more than 75% of cases still living four years after diagnosis in the 1997-1998 group. This Brazilian study demonstrates that even with regional inequalities for organizing an ideal structure of health care it is possible for a developing country to establish an effective system of universal and free access to HAART and having a significant increase in survival. / TEDE / BV UNIFESP: Teses e dissertações / BV UNIFESP: Teses e dissertações

The Innate immune system is the first line of defense against invading microbial pathogens. It is a fast-acting and non-antigen-specific defense system, which employs germline encoded surveillance systems capable of responding to a broad-spectrum of pathogens. The innate immune system involves a variety of immune cells, which express different profiles of surveillance or detection receptors. Upon sensing pathogens, these receptors trigger cell signalling to turn on transcription of inflammatory cytokines, chemokines, anti-microbial peptides and type I Interferons. These effectors have direct effects on the control of pathogen load and also activate the adaptive immune system, which is ultimately required to clear infections. The type I interferons (IFNs) are the principal cytokines strongly induced during infection with viruses and are required for direct control of viral replication and modulation of cells of the adaptive immune response. The signalling pathways induced in order to activate type I IFNs are dependent on the interferon regulatory factors (IRFs). Striving for survival, microbes have evolved various strategies to subvert/impair these critical defense molecules. In this thesis work, I have used Human Metapneumoviruses (HMPVs), a relatively newly described family of paramyxoviruses as model viruses to explore the role of pattern recognition receptors (PRRs) and the IRF family of transcription factors in the innate immune response. These studies revealed that the recognition of HMPV viral pathogen-associated molecular patterns (PAMPs) by immune cells is different in different cell types. Retinoic acid-inducible gene-I (RIG-I), a cytosolic RNA helicases senses HMPV-A1 virus for triggering type I IFN activation by detecting its 5’- triphosphate viral RNA in most human cells, including cell lines and primary monocytes. An exception to these findings was plasmacytoid dendritic cells (PDCs), where Toll-like receptor (TLR)-7 is the primary sensor involved in detecting HMPV viruses. By comparing the innate immune response to two HMPV strains, we found that these two closely related strains had very different immune stimulatory capabilities. HMPV-1A strain triggered type I IFNs in monocytes, PDCs and cells of epithelial origin. In contrast, a related strain, HMPV-B1 failed to trigger IFN responses in most cell types. Our studies suggested that the phosphoprotein (P) of HMPV-B1 could prevent the viral RNA from being detected by RIG-I, thus inhibiting the induction of type I IFN production in most cell type examined. This finding adds to our understanding of the mechanisms by which viruses are sensed by surveillance receptors and also unveils new means of viral evasion of host immune responses. Although IRFs are extensively studied for their role in regulating type I IFN activation, especially in TLR and RIG-I like receptor (RLR) signalling pathways upon viral infection, a clear understanding of how this family of transcription factors contributes to anti-viral immunity was lacking. Studies conducted as part of this thesis revealed that in addition to IRF3 and IRF7, which play a central role in anti-viral immunity downstream of most PRRs (e.g. TLRs, RLRs, DNA sensors), the related factor IRF5 was also an important component of innate anti-viral defenses. Using IRF5-deficient mice we studied in detail the role of IRF5 in coordinating antiviral defenses by examining its involvement in signalling downstream of TLRs. These studies led us to examine the role of IRF5 in the regulation of type I IFNs as well as inflammatory cytokines in different cell types. While most TLRs that induced IFNβ showed normal responses in IRF5-deficient mice, CpG-B-induced IFNβ production in CD11c+CDCs isolated from mouse spleen but not those generated in vitro from bone marrow required IRF5. This was in contrast to responses with lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (polyIC), ligands for TLR4 and 3, respectively. Moreover, we found that in contrast to IRF3 and/or IRF7, IRF5 was important in coordinating the expression of inflammatory cytokines such as TNFα downstream of some TLRs. In addition to our studies to examine the requirement for IRF5 in TLR signaling, we also showed that muramyl peptide (MDP) from Mycobacterium tuberculosis (Mtb) could activate type I IFNs via IRF5. This was the first evidence linking IRF5 to a non-TLR-driven pathway. IRF5 activation in this case was downstream of a novel nucleotide-binding oligomerization domain containing (NOD)-2/receptor-interacting serine-threonine kinase (RIP)-2 signaling pathway. Collectively, the studies outlined in this thesis have assisted in providing a framework to understand the role of TLRs, RLRs and IRFs in the immune response to paramyxoviruses and have unveiled new mechanisms of activation of the IRFs as well as new mechanisms by which pathogens subvert or evade these important innate defense mechanisms.

Introdução: O Protocol Aids Clinical Trial Group (PACTG 076), publicado em 1994, demonstrou que a utilização da zidovudina (ZDV) reduzia a taxa de transmissão materno-infantil do HIV (TMI HIV) de 25% para 8,3%. Atualmente a terapia antirretroviral (TARV) combinada associada a uma série de medidas pode reduzir a taxa de TMI HIV para menos de 2%. Embora o Ministério da Saúde preconize a adoção destas medidas, a TMI HIV ainda permanece acima dos níveis desejados, principalmente em nosso meio. De acordo com levantamento da Vigilância Epidemiológica do Município de Porto Alegre, no ano de 2012 a taxa TMI HIV foi de 2,9%. Objetivo: Avaliar os fatores que interferem na TMI HIV em um Hospital Universitário do Município de Porto Alegre/RS. Método: Trata-se de um estudo de coorte histórico, tendo como amostra 292 bebês nascidos de mulheres portadoras do HIV, cujos nascimentos ocorreram no Centro Obstétrico de um Hospital Universitário do Município de Porto Alegre/RS, entre 1º de janeiro de 2010 a 31 de dezembro de 2014. Resultados: Dos 292 bebês, cujas mães eram portadoras do HIV, 3,8% (n=11) foram contaminados. Destes 90,9% (n=10) nasceram por cesariana; 90,9% (n=10) tinham d37 semanas; 54,6% (n=6) receberam ZDV xarope isoladamente e 45,4% (n=5) receberam ZDV + nevirapina (NVP). Quatro gestantes cujos bebês foram contaminados apresentaram sífilis na gestação (36,4%). A má adesão a TARV (p<0,003), a carga viral d1.000 cópias/mL ou ignorada no 3º trimestre (p<0,001) e o CD4 <500 células/mm3 (p<0,046) no terceiro trimestre foram significativamente associados a maior TMI HIV. Conclusão: Os fatores associados significativamente a TMI HIV foram à má adesão a TARV, a presença de sífilis na gestação, a carga viral d1000 cópias e o CD4 <500 células/mm³ no terceiro trimestre. / Introduction: Protocol Aids Clinical Trial Group (PACTG 076), published in 1994, demonstrated that the use of zidovudine (ZDV) had reduced the rate of mother-tochild transmission (MTCT) of HIV from 25% to 8.3%. Currently, a combined antiretroviral therapy (HAART) associated with a number of measures can reduce the rate of MTCT to less than 2%. Although the Ministry of Health recommends the adoption of these measures, the MTCT still remains above desired levels, especially in our center. According to a survey of Epidemiological Surveillance of Porto Alegre, in 2012 the rate of MTCT was 2.9%. Objective: To evaluate the factors that interfere with MTCT from HIV-positive women who gave birth in a University Hospital of Porto Alegre/RS. Method: A historical cohort study, with a sample 292 babies from HIV infected mother whose delivery occurred at Obstetric Center of the University Hospital of Porto Alegre/RS, at period of January 2010 till December 2014. Results: Of 292 babies of women HIV positive, 3.8% (n=11) were infected. Of those 90.9% (n=10) was born by cesarean section; 90.9% (n=10) had d37 weeks; 54.6% (n=6) received only ZDV syrup and 45.4% (n=5) received ZDV+nevirapine (NVP). Four pregnant women whose babies were infected, mother had syphilis during pregnancy (36.4%). Poor adherence to HAART (p<0.003), viral load d1000 copies/mL or ignored in the third trimester (p<0.001) and CD4 <500 cells/mm3 (p<0.046) in the third trimester were significantly associated with higher MTCT. Conclusion: We conclude that the presence of syphilis in pregnancy, viral load d1000 copies/mL or ignored in the third trimester, the CD4 <500 cells/mm³ in the third trimester, poor adherence to HAART were significant factors for MTCT.

The word infection is a term which causes uncertainty among people. After all, it is not too long ago when people were dying in pandemics of plague, cholera or influenza. A human can be infected by viruses, bacteria, fungal organisms or parasites, many of which can infect several animal species. Diseases transmissible from animals to humans are called zoonoses. For efficient surveillance of zoonoses the cooperation of both veterinary and hygienic services is required. Its aim is timely detection and elimination of infection sources. It is based primarily on pointed veterinary and hygienic-epidemiological surveillance. In this thesis, I focused on the importance of collaboration of veterinary and public health services in the prevention of infectious diseases. One of the objectives of this work is to compare the occurrence of selected human zoonosis diseases in South Bohemia in the years 2002-2010. The second objective is to compare the extent of anti-epidemic measures adopted by veterinary services and public health authorities. In this thesis, a qualitative-quantitative research was utilized. The research aimed to determine the importance of cooperation of public health authorities and veterinary services in the prevention of infectious diseases. The quantitative part of the survey was conducted through the method of secondary data analysis. In the qualitative part of the survey, a semi-structured interview was held with the head of the epidemiology department of the District Hygienic Station of České Budějovice and with the director of the Regional Veterinary Administration for the South Bohemian Region. On the basis of the results of the research and implemented interviews I came to this conclusion: 1) The co-operation during the transmission of infectious diseases is ensured sufficiently. 2) The range of anti-epidemic measures adopted in the veterinary field and in the field of public health is sufficient. This thesis can serve as an educational material for students studying Bachelor or Master Degree of public health protection, because as public health service employees they will deal with issues related to veterinary supervision in practice.

The theoretical part of the thesis deals with the problems of imported infectious diseases. It comprises the factors influencing their incidence and the possibilities of specific and nonspecific prophylaxis and prevention. Further on, this part focuses on chosen infectious diseases, their epidemiological and clinical characteristics and trends in incidence. These infections are divided according to possibilities of specific prophylaxis. The thesis offers an overview of the possibilities of pre-travel consultancy in the South Bohemia region. The practical part of the thesis aims at the characterization of the incidence of imported infectious diseases in the districts within the region of South Bohemia in the years 2000 ? 2010. It also presents a survey of medical facilities and other subjects providing pre-travel consultancy and specific prophylaxis in the sphere of imported infectious diseases prevention. During the monitoring period, the total number of imported diseases which occurred in the region of South Bohemia and were registered in the EPIDAT system was 351. Out of this number, 205 cases occurred in the district of České Budějovice. (Český Krumlov ? 47, Jindřichův Hradec ? 25, Písek ? 2, Prachatice ? 17, Strakonice ? 31, Tábor ? 24). The most frequent imported infections were alimentary infections (240 cases, i.e.68 %); out of this, the most frequent cases were diseases caused by Salmonella (73 cases), Shigella (71 cases) and campylobacters (42 cases). The most frequent countries of origin were Egypt (43 cases), Croatia (42 cases) and India (30 cases). The number of medical facilities and other subjects providing pre-travelling consultancy and specific prophylaxis of infectious diseases rose from nine to thirteen during the period of monitoring. Four hypotheses were determined within this thesis. Hypothesis no.1 ? during the period of monitoring the total number of imported infectious diseases increased. Hypothesis no.2 ? during the period of monitoring the total number of infectious diseases imported from exotic destinations increased. Hypothesis no. 3 ? during the period of monitoring there was an increase in the number of the facilities and other subjects providing pre-travel advice, vaccination or other specific prophylaxis. Hypothesis no. 4 ? the increasing number of medical facilities or other subjects providing pre-travel advice, vaccination or other specific prophylaxis did not influence an increase in the number of imported infectious diseases during the period of monitoring. Only hypotheses no. 3 and no. 4 were proved.

The diploma thesis Infectious diseases of drug users is focused on monitoring the trend in the incidence of selected infectious diseases associated with the drug usage for a certain period of time. I have chosen viral hepatitis B and C for this monitoring because they belong to the most common infections typical for drug addicts at all. The issue of infectious diseases associated with intravenous drug usage is quite extensive therefore I tried to mention the most important information. Transmission of infectious diseases through intravenous drug usage is particularly insidious due to irrelevant length depending on time. The risk on non-compliance of basic hygiene measures is possible after a single application (using a sterile needle and syringe, a separate drug solution etc.). Another serious risk is that the viral hepatitis B and C can pass into the chronic stage, which significantly affects the quality of life and can also significantly reduce it. HIV infection is still incurable so it ends with the early death of the patient. If the syphilis is not treated and passes into the third stage it can ends up fatally. Infectious endocarditis is a serious life-threatening illness if not recognized and treated early. The diploma thesis has theoretical and practical part. In the theoretical part of this thesis I deal with various infectious diseases related to drug usage. I chose the most common disease according to the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA): viral hepatitis A, B, C, infections transmitted by sexual contact (syphilis, gonorrhea), HIV / AIDS, tuberculosis, infectious endocarditis and infections of the skin and soft tissues. Individual infections are divided into several chapters where the causative agent is described, mode of transmission, clinical picture, disease diagnosis, treatment and epidemiological measures. In the theoretical part I drew inspiration from the literature, internet sources and valid legal legislation. In the practical part I paid attention to the issue of occurrence of viral hepatitis B and C in the Pilsen Region for a certain period of time typical for intravenous drug users and the rest of the population. I compare these figures to the incidence of viral hepatitis in other regions of the Czech Republic for the same years. Data are analysed using quantitative research technique of secondary data analysis. Data are obtained from the information reporting system and records of transmissible diseases EPIDAT and from annual reports of the Czech Republic. The diploma thesis has one objective: to characterize and compare the trends of selected infectious diseases among drug users and the general population of the Pilsen Region in 2003-2011. Four hypotheses were defined: H1: The incidence of infectious diseases among drug users has been growing. H2: The frequency of infectious diseases among drug users in the Pilsen Region in the period 2003-2011 is comparable to the incidence of infectious diseases among drug users in the Czech Republic in the same time period. H3: The sickness rate of viral hepatitis B among drug users in the Pilsen Region in the period 2003-2011 is statistically more significant than among the general population of the Pilsen Region in the same time period. H4: The sickness rate of viral hepatitis C among drug users in the Pilsen Region in the period 2003-2011 is statistically more significant than among the general population of the Pilsen Region in the same time period. Due to lack of information in 2002, the objective of this diploma thesis and individual hypotheses had to be modified. Therefore the period relates to the year 2003-2011. Individual hypotheses were statistically tested to determine the statistical significance. The first and second hypothesis was refuted. A third hypothesis and fourth hypothesis was confirmed.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / From the HIV infection pregnant women and puerperas need specialized clinical follow-up, besides paying attention to their demands in the social dimension. The latter can be evaluated by the set of socio-environmental elements that act in a protective way, enabling individuals to deal adequately with everyday situations, such as care for the maintenance of prophylaxis of vertical HIV transmission. The objective was to evaluate if the social support perceived by pregnant women is different from that perceived by puerperal women living with HIV. This is a cross-sectional quantitative approach. The data collection site was Hospital Universitário de Santa Maria / RS, from April to November 2014, based on an instrument composed of a socio-demographic, clinical and obstetric characterization of pregnant women and puerperal women living with HIV; Social Support Scale for People Living with HIV / AIDS. The eligible population totaled 78 participants. The normality of the variables was evaluated through the Shapiro-wilk test; Descriptive statistics were used to present the baseline characteristics and the T test of independent samples. The Fischer's exact test, the linear logistic regression, was used to obtain estimates of OR and their respective confidence intervals (95% CI) for the intervening factors using the backward method. In all analyzes, significance level was set at p <0.05 and the statistical package SPSS version 21.0 was used. The population of pregnant women was 33.3% (n = 28) and 66.7% (n = 50) were puerperal. When the distribution between the pregnant and postpartum groups was evaluated, it was possible to verify that gestation is a protective factor when compared to the puerperium, since the puerperal women present a 8.8-fold chance of having low social support (OR: 8.80 ; CI: 1.01 - 16.76) and 6.0 times (OR: 6.00; CI: 1.53 - 10.48) for instrumental social support. Participants who are in the gestational period have a greater chance of presenting higher values of total and instrumental social support. In order to verify the reasons for the difference in total and instrumental social support, the characteristics of the groups were evaluated, a significant difference was observed in relation to the ingestion of alcoholic beverages (p = 0.007), use of ART before pregnancy was discovered (p = 0.044) (P = 0.050), support received from friends in emotional social support (p = 0.009), support received from friends in instrumental social support (p = 0.004). It is concluded that it is fundamental that the health professional knows and interacts with the social support network of these women, with the intention of implementing actions that allow them to overcome obstacles in maternal and child health care, especially during this period of family reorganization. / A partir da infecção pelo HIV gestantes e puérperas necessitam de acompanhamento clínico especializado, além de atenção as suas demandas na dimensão social. Esta ultima poderá ser avaliada pelo conjunto de elementos socioambientais que atuam de forma protetora, habilitando os indivíduos a lidarem de forma adequada com situações cotidianas, como os cuidados para a manutenção da profilaxia da transmissão vertical do HIV. Objetivou avaliar se o suporte social percebido por gestantes é diferente daquele percebido por puérperas vivendo com HIV. Trata-se de uma pesquisa de abordagem quantitativa do tipo transversal. O local de coleta de dados foi Hospital Universitário de Santa Maria/RS, no período de abril à novembro de 2014, a partir de um instrumento composto por questionário de caracterização sócio demográfica, clínica e obstétrica das gestantes e puérperas vivendo com HIV; Escala de Suporte Social para Pessoas Vivendo com HIV/aids. A população elegível totalizou 78 participantes. Foi avaliada a normalidade das variáveis por meio do teste Shapiro-wilk; foi utilizada estatística descritiva para apresentação das características basais e o teste T de amostras independentes. Realizou-se o teste exato de Fischer, a regressão logística linear para obter estimativas de OR e seus respectivos intervalos de confiança (IC 95%) para os fatores intervenientes, utilizando método backward. Em todas as análises, adotou-se nível de significância p < 0,05 e utilizou-se o pacote estatístico SPSS versão 21.0. A população de gestantes foi de 33,3% (n = 28) e 66,7% (n = 50) eram puérperas. Ao ser avaliado a distribuição entre os grupos de gestante e puérperas foi possível verificar que gestação é fator de proteção quando comparado ao puerpério, visto que as puérperas apresentam uma chance de 8,8 vezes de ter baixo suporte social geral (OR: 8,80; IC: 1,01 – 16,76) e 6,0 vezes (OR: 6,00; IC: 1,53 – 10,48) para suporte social instrumental. As participantes que estão no período gestacional possuem chance maior que apresentarem valores superior de suporte social total e instrumental. Para verificar os motivos de diferença no suporte social total e instrumental foi avaliado as características dos grupos, foi verificado diferença significativa com relação de ingestão de bebidas alcoólicas (p = 0,007), utilização de TARV antes da descoberta da gestação (p = 0,044), o grau de satisfação com apoio nas atividades para o dia a dia (p = 0,050), apoio recebido de amigos no suporte social emocional (p = 0,009), apoio recebido de amigos no suporte social instrumental (p = 0,004). Conclui-se que é fundamental que o profissional de saúde conheça e interaja com a rede de suporte social dessas mulheres, com o intuito de implementar ações que as permitam superar os obstáculos nos cuidados em saúde tanto materno e infantil, principalmente nesse período de reorganização familiar.

Made available in DSpace on 2016-06-02T19:48:24Z (GMT). No. of bitstreams: 1 6041.pdf: 1115025 bytes, checksum: d41bdc7a01ec6d45f7a7254522908dde (MD5) Previous issue date: 2014-02-20 / Universidade Federal de Minas Gerais / There is a lot of care to prevent vertical transmission of human immunodeficiency virus (HIV). A parent / caregiver must feel safe and instructed to realize them and so do not increase the exposure of the child. Not only the lack of guidelines, as well as those incomplete or not understood by the mother, can jeopardize the success of treatment and weaken the care given to the child. From this, the motivation for this research that had as general objective to analyze the experience of mothers or caregivers in relation to child care to reduce the risk of vertical transmission of HIV and specific objectives to analyze the experience of families in relation to child care to reduce the risk of HIV transmission, with emphasis on the beginning of this trajectory; to know the experience of mother in relation to child care to reduce the risck of postnatal transmission of HIV; to identify the social network / support of caregivers for children exposed to HIV in the postnatal period; and analyzed the experience of women living with HIV in relation to non- breastfeeding of the son. We used the perspective of Symbolic Interactionism as a theoretical and a descriptive qualitative approach. Data collection was performed in a specialized assistance service for HIV / AIDS in Northeastern Brazil, with 24 mothers, 5 fathers and 7 caregivers (grandparents, aunts and grandmother) of infants born to HIV-infected mothers. As selection criteria for the study, the participant should be caregiver and have children aged up to 18 months, which did not have a complete definition of HIV infection as well as make up in that service. Applied to semi-structured interviews and inductive content analysis, so the themes, categories and subcategories were defined by means of which the temporal dimension of the experience of care for HIV-exposed child, revealed by a trajectory of seizure, was understood. This trajectory starts to the become a mother until the definition of the child's diagnosis, fraught with memorable moments represented by pregnancy, the first month of the child's life and expectations regarding diagnostic disclosure also with regard to the child's future. The results were organized in four scientific papers that comprise the set of related categories. The 1st article brought the category becoming a mother with HIV, related to pregnancy, where the mother imagines her child with HIV, revive the situation before past experience and want the child, in spite of everything. The 2nd article has categories have solitary experience handling of antiretroviral therapy, be attentive to the care of the child, want to omit the presence of HIV and look to the future and fear of disease. The 3rd article met the categories go to specialized service in the dark, have a hope in the specialized service and has the fragile network of support. The 4th article included the category having the dream of breastfeeding frustrated, in which the mother suffers from the inability to breastfeed and have to accept the imposition of infant formula. This study showed different patterns of implementation of the child's treatment, which deserves special attention to ensure no vertical transmission of HIV. The stigma attached to the disease, the fragility of the support network, the frustration of the mother for not breastfeeding, among other feelings such as fear, guilt, sadness, loneliness, joy, relief and faith were part of the care experience. Even with the difficulties in this trajectory, caregivers showed hope in the negative diagnosis of children and commitment to compliance with treatment. The support from some family members of people living with HIV, the physician expert service, and especially of God, did they cope with the situation. / Há uma série de cuidados para evitar a transmissão vertical do vírus da imunodeficiência humana (HIV). A mãe/cuidador deve se sentir segura e instruída para realizá-los e assim não aumentar a exposição da criança. Não apenas a ausência de orientações, como também aquelas incompletas ou não compreendidas pela mãe, podem comprometer o sucesso do tratamento e fragilizar o cuidado dispensado à criança. A partir disso, surgiu a motivação para a realização desta pesquisa que teve como objetivo geral analisar a experiência de mães ou cuidadores em relação ao cuidado à criança para reduzir o risco de transmissão vertical do HIV e objetivos específicos analisar a experiência de familiares em relação ao cuidado à criança filha de mãe soropositiva para o HIV para reduzir o risco de transmissão do HIV, com ênfase no início desta trajetória; conhecer a experiência da mãe em relação ao cuidado à criança para reduzir o risco de transmissão pós-natal do HIV; identificar a rede social/apoio do cuidador da criança exposta ao HIV no período pós-natal; e analisar a experiência de mães que vivem com HIV em relação a não amamentação do filho exposto ao HIV. Utilizou-se a perspectiva do Interacionismo Simbólico como referencial teórico e uma abordagem metodológica qualitativa descritiva. A coleta dos dados foi realizada em um serviço de assistência especializado em HIV/AIDS, no Nordeste do Brasil, com 24 mães, 5 pais e 7 cuidadores (avós, tias e bisavó) de crianças nascidas de mães infectadas pelo HIV. Como critério de seleção para o estudo, o participante deveria ser cuidador e ter crianças na faixa etária de até 18 meses, que não possuísse a completa definição de infecção pelo HIV, bem como fazer acompanhamento no referido serviço. Aplicou-se a entrevista semiestruturada e a análise de conteúdo indutiva; assim, foram definidos os temas, categorias e subcategorias por meio dos quais a dimensão temporal da experiência de cuidado à criança exposta ao HIV, revelada por uma trajetória de apreensão, foi compreendida. Esta trajetória se inicia ao tornarse mãe até a definição do diagnóstico da criança, permeada de momentos marcantes representados pela gestação, pelo primeiro mês de vida da criança e expectativas em relação à revelação do diagnóstico, também no que diz respeito ao futuro da criança. Os resultados foram estruturados no formato de quatro artigos científicos que englobaram o conjunto de categorias afins. O 1º artigo trouxe a categoria tornar-se mãe com HIV, relacionada ao período gestacional, em que a mãe imagina o filho com HIV, revive a situação diante de experiência pregressa e deseja a criança, apesar da tudo. O 2º artigo tem as categorias ter experiência solitária de manuseio da terapia antirretroviral, estar atenta aos cuidados da criança, querer omitir a presença do HIV e olhar o futuro e temer a doença. O 3º artigo reuniu as categorias ir ao serviço especializado na obscuridade, ter no serviço especializado uma esperança e ter a rede de apoio fragilizada. O 4º artigo englobou a categoria ter o sonho de amamentar frustrado, em que a mãe sofre pela impossibilidade de amamentar e tem que aceitar a imposição da fórmula infantil. Este estudo mostrou diferentes padrões de implementação do tratamento da criança, o que merece atenção especial para a garantia da não transmissão vertical do HIV. O estigma em relação à doença, a fragilidade da rede de apoio, a frustração da mãe pela não amamentação, dentre outros sentimentos como medo, culpa, tristeza, solidão, alegria, alívio e fé fizeram parte da experiência de cuidado. Mesmo com as dificuldades encontradas nesta trajetória, os cuidadores mostraram esperança no diagnóstico de não infecção da criança e empenho para o cumprimento do tratamento. O apoio de alguns membros familiares, de pessoas que vivem com HIV, do médico do serviço especializado e, principalmente, de Deus, fizeram com que eles enfrentassem a situação.