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Copper bioaccumulation in blue mussels and periwinkles from marinasSjökvist, Tomas January 2019 (has links)
Copper (Cu) is a heavy metal that is essential for life but toxic at high concentrations. This toxic effect is used on boats to prevent biofouling on boat hulls by painting the hulls with antifouling paint that contain high levels of Cu. The Cu is slowly diffused out in the water and accumulated by animals higher up in the food chain. In order to test the effects of marinas on Cu bioaccumulation in invertebrates, I sampled molluscs at seven marinas and seven shore sites on the Swedish west coast. Two molluscs with different feeding behaviours, one grazer, the periwinkle (Littorina littorea) and one filter feeder, the blue mussel (Mytilus edulis) were used as study organisms. Both species were sampled at each location within 50 m from each other. Body Cu concentration of both species was measured with a Flame Atomic Absorption Spectrometer (FAAS). Cu concentration of periwinkle soft body tissue was generally higher than in mussel soft body tissue. In addition, periwinkle tissue Cu concentration reacted strongly positively to the presence of marinas, whereas mussel tissue Cu concentrations did not. This shows that contamination from marinas affects the grazing periwinkle but not the filter feeding blue mussel. Thus, for biomonitoring purposes, grazers may be more suited as indicator organisms.
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Estudo da presença, da função e das vias de produção da melatonina em invertebrados. / Melatonin in invertebrates: presence, functions and production pathways.Peres, Rafael 18 April 2013 (has links)
O tecido adiposo, através da leptina, desempenha um papel permissivo sobre a maturação sexual do indivíduo. Apesar da importância do tecido adiposo para a puberdade, pouco se sabe a respeito do processo de formação deste tecido neste período da vida. Estudos em humanos têm descrito a presença de resistência à insulina puberal. Assim, frente à importância do tecido adiposo para a puberdade, o objetivo do trabalho foi investigar a resposta do tecido adiposo à insulina no período puberal. Foram utilizados os coxins adiposos subcutâneo e periepididimal. Na puberdade, paralelamente à intolerância à glicose transitória que ocorreu nas semanas iniciais, e, portanto, prejuízo da utilização da glicose pelos tecidos insulino-dependentes, o tecido adiposo (SC e PE) teve sua responsividade normal à insulina e uma melhor capacidade de incorporação de glicose em lipídeos o que leva a crer que há um desvio da utilização deste substrato energético, a glicose, para a formação do tecido adiposo. / The adipose tissue is critical to puberty. Leptin exerts a permissive role to hypothalamic-hypophysial-gonadal maturation and, thereby, adipose tissue is necessary to pubertal development. Despite its importance to puberty, little is known about the process of adipose tissue formation during this period of life. Researches have described insulin resistance at pubertal period in humans. The adipose tissue importance to puberty added to pubertal insulin resistance described in humans lead us to investigate how the adipose tissue responds to insulin at this period since it is essential at this time. We assessed two distinct fat pads: the subcutaneous fat pad and the epididymal one. At the puberty, despite the temporary glucose intolerance and the constraint for glucose utilization by insulin-dependent tissues, the adipose tissue had normal insulin responsiveness plus an improved capacity to synthesize lipids from glucose which led us to hypothesize that glucose could have been deviated towards the adipose tissue.
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Avaliação ecotoxicológica do fármaco Triclosan para invertebrados marinhos / Ecotoxicological assessment of the pharmaceutical Triclosan for marine invertebratesCortez, Fernando Sanzi 10 February 2011 (has links)
Triclosan é um composto orgânico de baixa solubilidade que vem sendo utilizado em formulações de cremes dentais e faciais, xampu, sabonetes, embalagens de gêneros alimentícios e diversos tipos de materiais, tais como, adesivos, brinquedos, sapatos, selantes, tintas, colchão, roupas, pisos, toldos e rejuntes. O amplo uso deste composto deve-se à grande eficácia contra bactérias Gram negativas e Gram positivas. Por seu extenso uso, evidências da presença de Triclosan têm sido frequentemente relatadas em efluentes urbanos e industriais, águas superficiais e sedimentos de ambientes dulcícolas, estuarinos e marinhos, como também em organismos aquáticos como algas, peixes e mamíferos. Neste contexto, o presente estudo avaliou a toxicidade aguda e crônica de Triclosan para diferentes invertebrados marinhos de águas tropicais. Para tanto, ensaios de toxicidade aguda foram realizados com o copépodo Nitokra sp (mortalidade) e com o ouriço-do-mar Lytechinus variegatus (taxa de fertilização). Para a avaliação do efeito crônico, ensaios de toxicidade de curta duração (desenvolvimento embriolarval) foram realizados com o ouriço-do-mar L. variegatus e Perna perna. Além desses métodos, o ensaio do Tempo de Retenção do Corante Vermelho Neutro foi empregado com a finalidade de se avaliar os efeitos do Triclosan sobre a estabilidade da membrana lisossômica de hemócitos de P. perna. Na avaliação do efeito agudo, o valor médio da CL(I)50;96h encontrada para o copépodo foi de 0,20 mg.L-1 enquanto que o valor médio da CI(I)50;1h para ouriço-do-mar foi de 0,28 mg.L-1. Já na avaliação do efeito crônico, o valor médio da CI(I)50;24h para ouriço-do-mar foi de 0,14 mg.L-1 e para o molusco bivalve a média da CI(I)50;48h, foi de 0,13 mg.L-1. O efeito na estabilidade da membrana lisossômica de hemócitos de P. perna ocorreu em concentrações a partir de 12 ng.L-1. Estes resultados evidenciam o risco ecológico da introdução contínua desse composto em ambientes marinhos, e devem ser considerados para identificação de concentrações seguras e futura regulação do bactericida Triclosan na legislação ambiental nacional e internacional. / Triclosan is a low solubility organic compound that has been used in toothpastes, face cream, shampoos, soaps, food packages, and a variety of other materials such as stickers, toys, shoes, paints, clothes, tiles, awnings and grout. The reason for its intense use as biocide is its great efficacy against Gram-negative and Grampositive bacteria. Evidences of Triclosan presence in urban and industrial effluents, superficial waters and sediments from freshwater, estuarine, and marine environments, as well as aquatic organisms (algae, fishes, mammals) have been reported in the literature. In this context, the present study assessed the acute and chronic toxicity of Triclosan to different tropical marine invertebrates. Acute toxicity bioassays using the copepod Nitokra sp (mortality) and the sea-urchin Lytechinus variegatus (fertilization rate) were performed. Short-term chronic toxicity bioassays with Lytechinus variegatus and the bivalve mussel Perna perna were carried out in order to assess Triclosan chronic effects. Besides, the Neutral Red Retention Time assay was employed to evaluate the effect of Triclosan on the stability of lysosomal membrane of hemocytes of Perna perna. In the acute toxicity assays, the mean value of LC(I)50;96h obtained for the copepod was 0.20 mg L-1, whereas the mean value of IC(I)50;1h for the sea-urchin was 0.28 mg L-1. In the chronic toxicity assays, the mean value of IC(I)50;24h recorded for the seaurchin was 0.14 mg L-1, whilst for the bivalve mollusk the mean value of IC(I)50;48h was 0.13 mg L-1. The effect on the lysosomal membrane stability of Perna perna hemocytes started to occur from 12 ng L-1. The results evidence the ecological risk associated to the continuous introduction of Triclosan into marine aquatic environments and must be considered in the identification of safety concentrations and future regulation of this bactericide compound in national and international environmental legislation.
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Revisão e análise cladística do gênero Plesiopelma Pocock, 1901 (Araneae, Theraphosidae). / Revision and cladistic analysis of the genus Plesiopelma pocock, 1901 (Araneae, Theraphosidae).Nagahama, Roberto Hiroaki 22 April 2010 (has links)
O gênero Plesiopelma Pocock, 1901 é revisado taxonomicamente e realizada análise cladística de suas espécies. A matriz com 30 táxons terminais e 49 caracteres foi analisada com dois programas para reconstrução filogenética, considerando tanto caracteres aditivos como não-aditivos. As quatro análises apresentaram diferenças na topologia, porém três delas demonstram que Plesiopelma é um gênero monofilético. A diagnose e a distribuição geográfica das espécies são apresentadas. São consideradas válidas as espécies P. flavohirtum (Simon, 1889), P. semiaurantiacum (Simon, 1897),P. myodes Pocock, 1901, P. insulare (Mello-Leitão, 1923), P. physopus (Mello-Leitão, 1926), P. minense (Mello-Leitão, 1943) e P. simoni (Soares e Camargo, 1948). É proposta a sinonímia de P. rectimanum (Mello-Leitão, 1923) e P. longisternale (Schiapelli e Gerschman, 1942) com P. myodes. São reconhecidas seis espécies novas para o Brasil. É proposta a transferência de P. imperatrix Piza, 1976 para o gênero Megaphobema Pocock, 1901 e P. gertschi (Caporiacco, 1955) é considerada Incertae sedis. / The genus Plesiopelma Pocock, 1901 is taxonomically revised and a cladistic analysis of its species is done. The matrix with 30 terminal taxa and 49 characters was analysed with two programs for phylogenetic reconstruction. The four analyses showed different topologies on the internal group, however, three of them show Plesiopelma being a monophyletic genus. The diagnoses and geographic distribution of all species are presented. It is herein considered valid the species P. flavohirtum (Simon, 1889), P. semiaurantiacum (Simon, 1897), P. myodes Pocock, 1901, P. insulare (Mello-Leitão, 1923), P. physopus (Mello-Leitão, 1926), P. minense (Mello-Leitão, 1943) and P. simoni (Soares e Camargo, 1948). The species P. rectimanum (Mello-Leitão, 1923) and P. longisternale (Schiapelli & Gerschman, 1942) are considered junior-synonyms of P. myodes. It is recognized six new species from Brazil. It is proposed the transfer of P. imperatrix Piza, 1976 to Megaphobema Pocock, 1901 and P. gertschi (Caporiacco, 1955) is considered Incertae sedis.
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Mesofauna edáfica em plantios puros e mistos de Eucalyptus grandis e Acacia mangium / Soil mesofauna in pure and intercroped plantations of Eucalyptus grandis and Acacia mangiumZagatto, Maurício Rumenos Guidetti 07 March 2018 (has links)
A mesofauna edáfica compreende pequenos invertebrados que vivem nos primeiros centímetros do solo e na serapilheira. Sabe-se que o plantio de leguminosas com espécies arbóreas não fixadoras de nitrogênio melhora a fertilidade do solo, porém não se conhece o efeito desses plantios nos invertebrados edáficos. Diante disso, objetivou-se, com este estudo, avaliar o efeito de plantios puros e mistos de Eucalyptus grandis e Acacia mangium na mesofauna edáfica e estabelecer relações da mesofauna com os atributos químicos do solo e da serapilheira e os microbiológicos do solo, a fim de se construir um novo indicador de qualidade do solo. Para tanto, em outubro de 2015 (estação seca) e março de 2016 (estação chuvosa) foram avaliados os atributos físico-químicos da serapilheira (Ca, Mg, N, P, C, C/N, C/P, Mn, Cu, Fe, Zn, umidade), microbiológicos do solo (C mic, respiração do solo e atividade da desidrogenase), a umidade do solo e a mesofauna do solo e da serapilheira (riqueza, densidade e diversidade de mesofauna). Já os atributos químicos do solo (pH, Ca, Mg, C, N, P, Al, H+Al, Na, K) foram avaliados apenas em outubro. A média dos atributos foi comparada pelo teste de Tukey a 5%, enquanto que correlações, regressões e análises multivariadas foram feitas para estabelecer relações entre a mesofauna e os atributos do solo e da serapilheira e, posteriormente, construir um indicador geral de qualidade do solo. A fauna que habita a serapilheira, os atributos microbiológicos do solo e o indicador geral de qualidade do solo apresentaram maiores valores na estação chuvosa. A umidade foi muito correlacionada com os atributos biológicos do solo e da serapilheira. Foram também constatadas diferenças entre tratamentos, sendo que na estação seca há preferência da mesofauna pelo hábitat solo, possivelmente com a prevalência de relações mutualísticas entre microrganismos e mesofauna, enquanto há uma expressiva preferência da mesofauna pela serapilheira durante a estação úmida. / Soil mesofauna comprises small invertebrates that live in the first centimeters of the soil and in the litter. The consortium between leguminous trees and non-nitrogen-fixing tree species improves soil fertility, but the effect of these plantations on edaphic invertebrates is not known yet. Thus, we aimed at evaluating the effect of pure and mixed plantation of Eucalyptus grandis and Acacia mangium on the invertebrates that inhabit the soil and litter. We looked for correlations between those plantations, soil and litter chemical attributes and soil microbiological attributes to create a general indicator of soil quality in Acacia mangium (AC), Eucalyptus grandis (EU) and mixed plantations of Acacia and Eucalyptus (M). The chemical litter attributes evaluated were Ca, Mg, N, P, C, C/N, C/P, Mn, Cu, Fe, Zn, besides soil and litter moisture, soil microbiology (microbial carbon, soil respiration and dehydrogenase activity) and soil and litter mesofauna (richness, density and diversity) in two seasons: October 2015 (dry season) and March 2016 (rainy season). Soil chemical analyses (pH, Ca, Mg, C, N, P, Al, H+Al, Na, and K) were from samples collected in October. We made comparisons of the means between forest systems, and established a general indicator of soil quality based on regressions and multivariate analyses, to identify correlations between mesofaunaand chemical and microbiological attributes. Litter mesofauna, microbial activity and the general indicator of soil quality presented much higher values in the rainy season than in the dry season. Moisture correlated positively with most of the soil and litter biological attributes. There were few differences between the forest systems; although we observed a clear mesofauna preference for soil as habitat in the dry season, possibly linked to the prevailance of mutualistic interactions between soil mesofauna and microorganisms, while the mesofauna showed great preference for the litter as habitat during the moist season.
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Discovery of Loxosomella vivipara (Entoprocta: Loxosomatidae) in the Marine Sponge Hippospongia cf. gossypina (Porifera: Spongiidae) in the Florida KeysUnknown Date (has links)
Populations of a marine invertebrate symbiont were found on the outer surface and internal spaces of a keratose sponge from a shallow bay in the Florida Keys in May 2014. A total of 24 specimens of the seagrass and reef-dwelling sponge were collected between May 2014 and August 2015 to provide material to identify both host and symbiont, and elucidate information on the nature of the association. Based on a morphological analysis via light microscopy, histology, and scanning electron microscopy (SEM), and 99% similarity in aligned partial sequences from 28S and 18S nuclear ribosomal genes (rDNA), the symbiont was identified as the solitary entoproct Loxosomella vivipara Nielsen, 1966 (Entoprocta: Loxosomatidae). A partial sequence from the Internal Transcribed Spacer Region 2 (ITS2) of L. vivipara was registered to GenBank for the first time. The identity of the host sponge, based on a morphological investigation, is resolved as “velvet sponge” Hippospongia cf. gossypina Duchassing and Michelotti, 1864 (Demospongiae: Spongiidae). This is the first report of an entoproct commensal from Hippospongia cf. gossypina, a sponge that formerly had great commercial value when it was abundant throughout the Bahamas, Florida Keys and Gulf of Mexico.
Other common sponge species at the study site were collected to investigate the host specificity of L. vivipara. Evidence that L. vivipara favorably selects the sponges Hippospongia cf. gossypina and Chondrilla nucula over other potential host sponges at the study site is provided. Commensalism is the most plausible justification for this relationship: L. vivipara is dependent on sponges for protection and food particles, while the sponges are unaffected by its presence. Further evidence of host-specific inquilinism is provided for L. vivipara associated with Hippospongia cf. gossypina, but not for L. vivipara associated with C. nucula. An inquilinistic association between an entoproct and sponge is a rare discovery only mentioned in one previous study. Sponge aquiferous qualities such as aperture and canal size, canal and choanocyte chamber arrangement, seawater pumping rate, and food particle size selection are likely factors that inhibit or enable inquilinism in sponge-entoproct associations – a potential topic for future researc / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2016. / FAU Electronic Theses and Dissertations Collection
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Impacts of anthropogenic noise on behaviour, development and fitness of fishes and invertebrates / Étude des bruits anthropiques sur le comportement, le développement et le fitness des poissons et des invertébrésNedelec, Sophie 17 August 2015 (has links)
Dans la première partie de ma thèse, je fournis un programme informatique qui permet aux utilisateurs de déterminer dans quelles circonstances ils doivent mesurer le mouvement des particules. J’explique comment ces mesures peuvent être effectuées et fournissent un programme pour analyser ce type de données. La partie principale de ma thèse comprend des expériences portant sur l'impact de l'exposition répétée au bruit du trafic, la source anthropique la plus courante de bruit dans l'environnement marin, sur les poissons et les invertébrés pendant leur développement. Je démontre qu’une variété de comportements a été touchée par le bruit. J’ai aussi trouvé que le bruit prévisible peut conduire à des impacts différents sur le développement par rapport au bruit imprévisible, mais que certaines espèces de poissons peuvent être en mesure de s'habituer au bruit du trafic, tandis que d'autres non. En outre, je trouve que le développement et la survie des limaces de mer peuvent être impactés négativement par le bruit des bateaux. Les poissons et les invertébrés constituent une source de nourriture vitale pour des millions de personnes et constituent des liens essentiels dans de nombreux réseaux trophiques. L'étude de leur comportement, de développement et de remise en forme peut nous donner un aperçu des impacts de la population et le niveau de bruit de la communauté qui sont pertinents pour la survie des espèces et l'évolution. Le développement de certaines des nouvelles idées et techniques abordées dans cette thèse nous permettra de faire progresser ce domaine vital de la recherche. / As the first part of my thesis, I provide a computer program which allows users to determine under what circumstances they should measure particle motion; I explain how these measurements can be made and provide a program for analysing this type of data. The main part of my thesis comprises experiments investigating the impact of repeated exposure to traffic noise, the most common anthropogenic source of noise in the marine environment, on fishes and invertebrates during development. In all three chapters involving experiments on fish in tanks and in the field, I found that a variety of behaviours were impacted by traffic noise playback. I also found that predictable noise can lead to different impacts on development compared to unpredictable noise, but that some species of fish may be able to habituate to traffic noise, while others suffer lower survival. Further, I found that the development and survival of seahares can be negatively impacted by traffic-noise playback. Fishes and invertebrates provide a vital food source to millions of people and form crucial links in many food webs; studying their behaviour, development and fitness can give us an insight into population and community level impacts of noise that are relevant to species survival and evolution. Developing some of the novel ideas and techniques discussed in this thesis will enable us to advance this vital area of research.
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The effects of anthropogenic noise playbacks on marine invertebratesWale, Matthew A. January 2018 (has links)
Anthropogenic sound has profoundly changed the acoustic environment of aquatic habitats, with growing evidence that even a short exposure to man-made sound sources can negatively affect marine organisms. Marine invertebrates have received little attention regarding their responses to anthropogenic sound, despite their pivotal role in marine ecosystems, and preliminary evidence of their sensitivity. In this thesis, I critically review the methods used in studies investigating the effects of anthropogenic noise on marine invertebrates. I identify methodological trends that have developed along the timeline of this topic, and use this information to suggest three research strategies to further the development of research in this field. From this review, current knowledge gaps are identified, and two main routes to address them are taken in this thesis. Firstly, to address the shortage of particle motion data in anthropogenic sound literature, two new low-cost and easily accessible particle motion sensors were developed and tested, one of them at 0.1% of the cost of currently commercially available models. These sensors will allow the measurement and reproduction of the sound fields experienced by marine invertebrates in bioacoustic research, even when research budgets are tight. Particle motion is the main sound component detected by invertebrates, yet neglected in many aquatic bioacoustical studies. Secondly, to expand on the comparably small quantity of research investigating the effects of anthropogenic sound on marine invertebrates, a series of controlled laboratory experiments were conducted. Two commercially and ecologically important model species were chosen, the blue mussel Mytilus edulis, representing sessile benthic invertebrates, and the European lobster Homarus gammarus, representing mobile benthic invertebrates. Experiments on M. edulis were conducted taking a mechanistic, integrative approach to investigate the effects of sound on multiple levels of biological organisation, including biochemistry, physiology, and behaviour. The ultimate aim was to understand the underlying drivers behind, and interactions between, responses. Comet assay analysis of haemocytes and gill cells demonstrated a significant six-fold higher single strand breakage in the DNA of cells of mussels exposed to ship-noise playback, compared to those kept under ambient conditions. Superoxide dismutase analysis did not identify an excess of superoxide ions, and glutathione, and glutathione peroxidase assays showed no increase in either glutathione or glutathione peroxidase. TBAR assays however revealed 68% more thiobarbituric acid reactive substances, indicating lipid peroxidation in the gill epithelia of noise exposed specimens. Algal clearance rates and oxygen-consumption rates of noise-exposed mussels were significantly lower (84% reduction and 12% reduction respectively), than those of control animals, while valve gape was significantly (60%) wider. This seemingly converse reaction indicates a shock response in mussels with the onset of noise exposure. Finally, at the genetic level, heat shock protein 70 expression was investigated, but no change was identified during noise exposure. Investigation into the noise induced behavioural responses of H. gammarus suggests seasonal differences in behaviour, using movement as a metric, in response to anthropogenic noise playbacks. During both summer and winter exposures, lobsters spent most time away from the highest noise area (98% of the observed time in summer and 78% in winter). The observed seasonal differences in the time spent in the highest noise area (2% in summer and 22% in winter) could be linked to the lobsters' requirement for shelter during winter. This requirement seems to have had a stronger influence over the animals' behaviour than any desire to avoid high noise levels. The information generated in this thesis can be used by researchers working in the field of marine sound to develop well rounded studies exploring the effects of sound on not only marine invertebrates but other faunal groups as well. The construction details provided to produce low cost particle motion sensors, will allow bioacoustic researchers to easily include particle motion measurements in all future studies investigating the effects of sound on fish and invertebrates. The results of the conducted mussel and lobster experiments evidence how multiple aspects of invertebrate biology can be affected by noise. The observed impacts on both sessile and mobile life forms highlight that the effects of noise cannot be fully understood before a wide range of species with different biological and ecological traits have been studied. The integrative approach to noise research used here can serve as a model for other species, and the results pooled to inform governments and industry of the effects of anthropogenic noise in the marine environment.
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Mesofauna edáfica em plantios puros e mistos de Eucalyptus grandis e Acacia mangium / Soil mesofauna in pure and intercroped plantations of Eucalyptus grandis and Acacia mangiumMaurício Rumenos Guidetti Zagatto 07 March 2018 (has links)
A mesofauna edáfica compreende pequenos invertebrados que vivem nos primeiros centímetros do solo e na serapilheira. Sabe-se que o plantio de leguminosas com espécies arbóreas não fixadoras de nitrogênio melhora a fertilidade do solo, porém não se conhece o efeito desses plantios nos invertebrados edáficos. Diante disso, objetivou-se, com este estudo, avaliar o efeito de plantios puros e mistos de Eucalyptus grandis e Acacia mangium na mesofauna edáfica e estabelecer relações da mesofauna com os atributos químicos do solo e da serapilheira e os microbiológicos do solo, a fim de se construir um novo indicador de qualidade do solo. Para tanto, em outubro de 2015 (estação seca) e março de 2016 (estação chuvosa) foram avaliados os atributos físico-químicos da serapilheira (Ca, Mg, N, P, C, C/N, C/P, Mn, Cu, Fe, Zn, umidade), microbiológicos do solo (C mic, respiração do solo e atividade da desidrogenase), a umidade do solo e a mesofauna do solo e da serapilheira (riqueza, densidade e diversidade de mesofauna). Já os atributos químicos do solo (pH, Ca, Mg, C, N, P, Al, H+Al, Na, K) foram avaliados apenas em outubro. A média dos atributos foi comparada pelo teste de Tukey a 5%, enquanto que correlações, regressões e análises multivariadas foram feitas para estabelecer relações entre a mesofauna e os atributos do solo e da serapilheira e, posteriormente, construir um indicador geral de qualidade do solo. A fauna que habita a serapilheira, os atributos microbiológicos do solo e o indicador geral de qualidade do solo apresentaram maiores valores na estação chuvosa. A umidade foi muito correlacionada com os atributos biológicos do solo e da serapilheira. Foram também constatadas diferenças entre tratamentos, sendo que na estação seca há preferência da mesofauna pelo hábitat solo, possivelmente com a prevalência de relações mutualísticas entre microrganismos e mesofauna, enquanto há uma expressiva preferência da mesofauna pela serapilheira durante a estação úmida. / Soil mesofauna comprises small invertebrates that live in the first centimeters of the soil and in the litter. The consortium between leguminous trees and non-nitrogen-fixing tree species improves soil fertility, but the effect of these plantations on edaphic invertebrates is not known yet. Thus, we aimed at evaluating the effect of pure and mixed plantation of Eucalyptus grandis and Acacia mangium on the invertebrates that inhabit the soil and litter. We looked for correlations between those plantations, soil and litter chemical attributes and soil microbiological attributes to create a general indicator of soil quality in Acacia mangium (AC), Eucalyptus grandis (EU) and mixed plantations of Acacia and Eucalyptus (M). The chemical litter attributes evaluated were Ca, Mg, N, P, C, C/N, C/P, Mn, Cu, Fe, Zn, besides soil and litter moisture, soil microbiology (microbial carbon, soil respiration and dehydrogenase activity) and soil and litter mesofauna (richness, density and diversity) in two seasons: October 2015 (dry season) and March 2016 (rainy season). Soil chemical analyses (pH, Ca, Mg, C, N, P, Al, H+Al, Na, and K) were from samples collected in October. We made comparisons of the means between forest systems, and established a general indicator of soil quality based on regressions and multivariate analyses, to identify correlations between mesofaunaand chemical and microbiological attributes. Litter mesofauna, microbial activity and the general indicator of soil quality presented much higher values in the rainy season than in the dry season. Moisture correlated positively with most of the soil and litter biological attributes. There were few differences between the forest systems; although we observed a clear mesofauna preference for soil as habitat in the dry season, possibly linked to the prevailance of mutualistic interactions between soil mesofauna and microorganisms, while the mesofauna showed great preference for the litter as habitat during the moist season.
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Metabólitos secundários bioativos de invertebrados marinhos: isolamento, determinação estrutural e atividades biológicas / Bioactive secondary metabolites from marine invertebrates: isolation, structure determination and biological activitiesKossuga, Miriam Harumi 21 October 2008 (has links)
Ao longo dos últimos 20 anos os organismos marinhos se tornaram uma das fontes mais interessantes para o isolamento de produtos naturais biologicamente ativos. Apesar disso, os organismos marinhos da costa brasileira foram muito pouco explorados como fonte de metabólitos secundários. A presente investigação se insere no âmbito de dois projetos temáticos, o primeiro desenvolvido entre 2002 e 2006 e o segundo em andamento, que têm por objetivo melhor conhecer as potencialidades de organismos marinhos da costa brasileira como fonte de metabólitos secundários biologicamente ativos. Assim, foram investigados extratos bioativos de cinco espécies de invertebrados marinhos: três ascídias, uma esponja e um octocoral, todos oriundos da costa brasileira. O fracionamento dos extratos destes animais resultou no isolamento de 15 compostos, dos quais sete inéditos na literatura. A partir do extrato bruto da ascídia Clavelina oblonga foram isolados dois compostos: a 5-[3,5-dibromo-4([2-oxo-5-oxazonidinil)]metoxifenil]2-oxazolidinona e o (2S,3R)- 2-amino-3-dodecanol. Este último apresentou uma potente atividade antifúngica contra Candida albicans. Do extrato da ascídia Didemnum ligulum foram obtidos dois compostos: a asterubina e a N,N-dimetil-O-metiletanolamina. A investigação química do extrato da ascídia Didemnum sp. resultou no isolamento de quatro dicetopiperazinas modificadas denominadas rodriguesinas A e B, e N-acetil-rodriguesinas A e B. As rodriguesinas A e B foram obtidas na forma de mistura inseparável, e puderam ser identificadas por análises espectroscópicas detalhadas, inclusive por experimentos MS/MS. A mistura contendo as rodriguesinas A e B apresentou moderada atividade antibiótica contra um isolado clínico de Streptococcus mutans, contra S. mutans UA159 e Staphylococcus aureus ATCC6538. A partir do extrato bruto da esponja Plakortis angulospiculatus foram obtidos seis policetídeos: a plakortenona, a plakortina, o plakortídeo P, o 3,6 epoxi, 4,6,8 trietil-2,4,9-dodecatrienoato de metila, a espongosoritina A e o 3,6 epoxi, 4,6,8 trietil-2,4,-dodecadienoato de metila. Os compostos puros obtidos da esponja P.angulospiculatus foram avaliados em testes de atividade antiparasitária contra Leishmania chagasi e Trypanosoma cruzi, antineuroinflamatória e citotóxica frente a quatro diferentes linhagens de células tumorais. O composto plakortídeo P apresentou potente atividade leishmanicida altamente seletiva. Finalmente, a investigação do extrato bruto do octocoral Carijoa riisei resultou no isolamento de um único constituinte, o esteróide 18-acetoxipregna-1,4,20-trien-3-ona, na qual apresentou atividades tripanomicida e leishmanicida. / Throughout the last 20 years, marine organisms have become one of the most interesting sources for the isolation of biological active natural products. However, marine organisms from the Brazilian coastline have been largely underexplored as source of secondary metabolites. The present investigation was developed in the scope of two thematic projects, the first one during 2002 and 2006 the second one, still in progress, aims the discovery of the potentialities of marine organisms of the Brazilian coast as a source of biologically active secondary metabolites. Bioactive extracts of five species of marine invertebrates have been investigated: three ascidians, one sponge and one octocoral. The fractionation of these extracts led to the isolation of 15 compounds, of which seven are unprecedented in the literature. From the crude extract of the ascidian Clavelina oblonga two compounds have been isolated: [3,5-dibromo-4-[(2-oxo-5-oxazolidinyl)]methoxyphenyl]-2- oxazolidinone and (2S,3R)-2-aminododecan-3-ol. The last one displayed potent antifungal activity against Candida albicans. Two compounds have been obtained from the crude extract of the ascidian Didemnum ligulum: asterubine and N,N-dimethyl-O-methylethanolamine. The chemical investigation of the extract of the ascidian Didemnum sp. resulted in the isolation of four modified diketopiperazines: rodriguesines A and B, was well as N-acetil-rodriguesines A and B. Rodriguesines A and B have been obtained as an unseparable mixture, and have been identified by detailed analysis of spectroscopic data including MS/MS experiments. The mixture of the rodriguesines A and B displayed moderate antibiotic activity against a clinical isolate of Streptococcus mutans, against S. aureus mutans UA159 and Staphylococcus aureus ATCC6538. The crude extract of the sponge Plakortis angulospiculatus have been investigated and six polyketides have been isolated: plakortenone, plakortin, plakortide P, methyl 3,6-epoxy-4,6,8-triethyl-2,4,9-dodecatrienoate, spongosoritin A and methyl 3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate. The polyketides isolated from the sponge P.angulospiculatus have been evaluated in tests of antiparasitic activity against Leishmania chagasi and Trypanosoma cruzi, antineuroinflammatory and cytotoxic against four different human cancer cell lines. Plakortide P exhibited potent and highly selective leishmanicidal activity. Finally, the chemical investigation of the crude extract from the octocoral Carijoa riisei resulted in the isolation of a single constituent, the known steroid 18- acetoxypregna-1,4,20-trien-3-one, which displayed cytotoxic, antitrypanosomal and antileishmanial activity.
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