Christian Louboutin é um designer da moda, que trata da estética sob a ótica do processo criativo, um produtor de artigos de luxo, que atua em um nicho de mercado com acessórios da moda de luxo, para homens e mulheres com alto poder aquisitivo. Faremos um estudo de caso único, delimitado ao assunto da estética e o design nos sapatos femininos da marca Christian Louboutin, a fim de buscarmos respostas através de alguns questionamentos para as seguintes questões de pesquisa: 1) Como a estética e o design nos sapatos da marca Christian Louboutin desempenham um papel importante no despertar da imagem, de poder, da elegância, da sensualidade e do fetiche nas clientes Louboutin? 2) Como os elementos resultantes dos sentimentos de prazeres e das experiências sensoriais subliminares estimuladas por atributos estéticos, dão origem a diversas sensações e emoções que servem de incentivo no processo de compra? O objetivo deste estudo será o de encontrar os principais atributos estéticos que expressam determinado nível de beleza nos sapatos Christian Louboutin, aqueles que resultam em experiências emocionais sensoriais subliminares que influem nos gostos e nos gastos das clientes, quando compram os sapatos de luxo da marca. A metodologia deste trabalho consistirá de um estudo de caso único que utilizará os métodos de coleta de dados: observação e entrevista. As entrevistas serão realizadas com seis clientes no ponto de venda, associada à determinada fundamentação teórica. Os resultados obtidos demonstraram que não ocorreram divergências significativas nas respostas dadas pelas entrevistadas se contrapomos aos objetivos deste trabalho. Os resultados das entrevistas se alinham ao conceito de estética e do design dado pela fundamentação teórica, e por serem considerados os elementos mais admirados pelas clientes nos sapatos de luxo Christian Louboutin. A conclusão deste estudo de caso é que os elementos estéticos encantam e fascinam as clientes, despertam sensações de poder, paixões e fetiche, além de dar a sensação de ficarem mais lindas, sensuais e atraentes. A estética age como um elemento imprescindível à inovação e a diferenciação nos sapatos, garante a autenticidade da marca, servindo de conexão como o imaginário de suas clientes. / Christian Louboutin is a fashion designer who deals with the aesthetics from the perspective of the creative process, a producer of luxury goods, which operates in a niche market with luxury fashion accessories for men and women with high purchasing power. We\'ll make a single case study, defined the subject of aesthetics and design in women\'s shoes brand Christian Louboutin, to seek answers through some questions to the following research questions: 1) How the aesthetics and design in shoes brand Christian Louboutin play an important role in the image of awakening, power, elegance, sensuality and fetish in Louboutin customers? 2) How the resulting elements of the feelings of pleasure and subthreshold sensory experiences stimulated by aesthetic attributes, give rise to different sensations and emotions that serve to encourage the purchase process? The aim of this study is to find the main aesthetic attributes that express certain level of beauty in Christian Louboutin shoes, those that result in subliminal sensory emotional experiences that influence the tastes and spending of customers when they buy luxury brand shoes. The methodology of this work will consist of a single case study that will use the data collection methods: observation and interview. The interviews will be conducted with six customers at point of sale, associated with a particular theoretical framework. The results showed that no significant differences in the answers given by the respondents to counterpose the objectives of this work. The results of the interviews are aligned to the concept of aesthetics and design given by the theoretical basis, and consider the most admired elements by customers in luxury shoes Christian Louboutin. The conclusion of this case study is that the aesthetic elements delight and fascinate customers, arouse feelings of power, passion and fetish, and give the feeling of being the most beautiful, sexy and attractive. The aesthetic acts as an essential element for innovation and differentiation in shoes, guarantees the authenticity of the brand, serving as a connection to the imagination of its customers. Google Tradutor para empresas:Google Toolkit de tradução para appsTradutor de sitesGlobal Market Finder

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A partir d'un mémoire du master sur l'étude du mobilier issu du sanctuaire de Beire-le-Châtel « Le Pâtis du Letto » ainsi que de nouvelles études comme celle du matériel provenant du fanum de Lux « Le Bois Giraud », l'ambition de cette thèse est d'appréhender la population qui le long de la voie Lyon - Trèves en pays lingon à l'époque romaine et fréquentent ses lieux de culte. Le mobilier retrouvé dans les sanctuaires a été confronté à celui issu des fouilles d’établissements ruraux, permettant de distinguer fréquentation locale et voyageurs de passage. Enfin, l’étude permet de mettre en avant le rôle des agglomérations – chef-lieu de cité et agglomérations secondaires – et des axes de communication dans la genèse des lieux de culte lingons, mais aussi la place tenue par les établissements ruraux de moyen et fort statut dans mise en place du paysage religieux. / From a memory of a master on the study of the material from the sanctuary of Beire-le-Châtel "The Pâtis the Letto" as well as new studies such as material from the fanum Lux "Le Bois Giraud", the objective of this thesis is to understand the people who live along the Roman road Lyon - Trier on the civitates of the Lingoni et go on these places of worship. The material found in these sanctuaries was confronted with one of the rural settlements to distinguish local attendance and passing travelers. Finally, the study allows to emphasize the role of settlements - administrative center of the city and secondary towns - and communication routes in the genesis of Lingones’ places of worship but also the place held by the rural settlements of medium and high status in the implementation of the religious landscape.

The hunt for Dark Matter plays a truly critical role in contemporary physics. At both the largest and smallest scales, deep questions are being raised about the fundamental nature of the universe - questions that confirmation and then characterisation of particle dark matter will provide many answers to. This thesis presents some of the world's most sensitive searches to date for certain types of axion dark matter, axion-like particles, and two-neutrino double electron capture. These have been conducted using the Large Underground Xenon (LUX) experiment. Evidence for dark matter and physics beyond the Standard Model of particle physics is described in Chapter 1, while Chapter 2 gives an overview of proposed candidates for particle dark matter. The various experimental approaches being used to detect particle dark matter are presented in Chapter 3. Direct detection with time projection chambers plays a major role in this thesis, with particular interest in the LUX detector, that is described in its components and operations. Chapter 4 presents LUX direct searches for weakly interacting massive particles. Although I have contributed to these analyses, they are included for completeness only, as they are not part of my central work. The LUX collaboration's searches for axion dark matter and axion-like particle have delivered world-leading results on the axion-electron coupling constant. These results, that I personally led and which have been published in Physics Review Letters, are presented in Chapter 5, along with sensitivity studies, also led by me, made for the future LUX-ZEPLIN experiment. Finally, a search for two-neutrino double electron capture of 124Xe, that I performed using LUX data to extract a limit on the half life of the process, is presented in Chapter 6. Although being allowed by the Standard Model, two-neutrino double electron capture shares the matrix element calculation framework with the neutrinoless channel of the same process, becoming of great interest in the scope of neutrino physics. Conclusions follow and close the thesis.

L’analyse des processus de création de l’écrivain américain William Burroughs et de l’artiste brésilien Cildo Meireles construit deux figures exemplaires de l’artiste en tant que sujet politique. Les choix faits par ces deux artistes pendant le processus de réalisation de leurs œuvres déclarent en effet leur position face à la littérature, à l’art et à la réalité. Ils les construisent en tant que sujets-artistes et produisent des rencontres qui (re) configurent l’espace artistique. Ils renouvellent les pratiques de création. Nous étudions ces processus avec pour outils différentes notions théoriques. Outre celle de la politique, qui, selon Jacques Rancière, est le processus de l’égalité ; celle du sujet, définie par Alain Badiou, comme une construction qui se produit quand un événement arrive ; et celle de choix qui, selon Slavoj Zizek, ne laisse pas intacte la subjectivité du sujet. Nous essayons ainsi de définir le caractère politique des choix faits par ces deux artistes et de mettre en évidence l’idée de l’art et de la politique que leurs œuvres mobilisent.Nous étudions le processus de création de Burroughs, de son premier roman jusqu’à la découverte du cut-up et l’écriture de son texte Last Words. Nous analysons le processus de création des œuvres de Meireles : Insertions dans les circuits idéologiques, œuvre qui se constitue dans un processus en marche ; et Le sermon sur la montagne : Fiat lux. Construction d’une situation : transformation d’un espace en une bombe potentielle.Malgré leurs différences, ces œuvres ont une procédure de réalisation similaire, une sorte de travail tabulaire, de collage d’éléments hétérogènes. / The analysis of the creation process of both Americain writer Willam Burroughs and Brazilian artist Cildo Meireles construct two exemplary figures of the artist as political subject. The choices made by these artistes during the process of realization of their works declare indeed their position on literature, art and reality. Their choices construct them as artists-subjects and produce encounters that (re) configure the art space. They renew the creative practices. We study these processes with some theoretical tools: such as the notion of politics which according to Jacques Rancière, is the process of equality; the notion of subject, defined by Alain Badiou as a construction that is produced when an event happens; and the notions of choice which, according to Slavoj Zizek, does not leave intact the subjectivity of the subject. Thus we try to define the political nature of the choices made by these artists and to highlight the idea of art and politics that their works mobilize. We study the process of creation of Burroughs, from his first novel until his discovery of cut-up technique and the writing of his text Last Words. We analyze the process of creation of Meireles’s works: Insertions into Ideological Circuits, a work that is established as a running process and The Sermon on the Mount: Fiat Lux that is the construction of a situation. It is the transformation of a space into a potential bomb. Despite their differences these works have a similar implementations procedures a kind of a tabular work, a collage of heterogeneous elements.

Opportunistic bacterial pathogens are present in the intestines of all mammals. These bacteria are symbionts to a certain extent, but under certain conditions these organisms can be deadly. Intestinal opportunistic pathogens encompass many genera and include organisms such as those in the Bacteroides fragilis group (i.e. B. fragilis and B. thetaiotaomicron), Escherichia coli, and Clostridium perfringens, resulting in an array of diseases and serious health risks. Typically these diseases affect individuals in poor or weakened health (elderly, immuno-compromised, neonates, etc.) but can affect healthy individuals as well. The intestinal tract is the main area of infection for these bacteria, however some of these organisms can be involved in wound infections, septicemia, urinary tract infections, and meningitis. This study focused on three areas: 1) Analysis of differences in gene expression between Bacteroides and Escherichia coli, in order to learn more about promoter structure, 2) Establishment of a diabetic mouse model for use in examining bacterial synergy during a polymicrobial infection, and 3) Characterization of Escherichia coli 360A and evaluation of the role of several virulence factors and environmental modulators in the pathogenesis of this strain. We used a newly developed lux gene reporter to evaluate gene expression in Bacteroides. We observed that there are barriers in both transcription and translation initiation that appear to limit the expression of foreign genes in Bacteroides. We were able to establish a mouse model for studying synergy during a polymicrobial infection and observed that E. coli 360A provided synergy towards B. fragilis NCTC 9343. These experiments also showed that the longer a mouse is afflicted with the complications of diabetes the more susceptible it is to polymicrobial infections. Systemic infections were used to evaluate the contribution of several virulence factors and environmental modulators in the pathogenesis of E. coli 360A. The results showed that a strain lacking both virulence factors CNF1 and HlyA, the terminal oxidase cytochrome o, or a double cyo/cyd mutant were, deficient in survival in the spleen, but not the liver of BALB/c mice. / Ph. D.

Whole cell biosensors have been extensively used for monitoring toxicity and contamination of compounds in environmental biology and microbial ecology. However, their application in the pharmaceutical and cosmetics industries for preservative efficacy testing (PET) has been limited. According to several pharmacopoeias, preservatives should be tested for microbial activity using traditional viable count techniques; the use of whole cell microbial biosensors potentially provides an alternative, fast, and efficient method. The aim of the study was to construct and develop whole cell microbial biosensors with Pseudomonas aeruginosa ATCC 9027. Constitutive promoters: PlysS, Pspc, Ptat, Plpp and PldcC and the lux-cassette were inserted into plasmid pME4510 and transformed into P. aeruginosa ATCC 9027 cells to produce bioluminescent strains. Plasmids were found to be maintained stably (~50 copies per cell) throughout the growth and death cycle. The novel bioluminescent strains were validated in accordance with the pharmacopoeia using bioluminescence detection and quantification followed by comparison with the traditional plate counting method. The bioluminescent method was found to be accurate, precise and equivalent at a range of 103 – 107 CFU/mL, as compared with plate counting. Recovery of bacterial cells was quantified using bioluminescence; this method proved to be accurate with percentage recoveries between 70-130% for all bioluminescent strains. The method was also more precise (relative standard deviation less than 15%) than the traditional plate counting method or the ATP bioluminescent method. Therefore, the bioluminescent constructs passed/exceeded pharmacopoeial specified criteria for range, limit of detection, accuracy, precision and equivalence. Physiology of the validated bioluminescent strains was studied by assessing the growth and death patterns using constitutive gene expression linked with bacterial replication. Promoter strengths were evaluated at various stages of the growth and death pattern and related to promoter sequences. PlysS, Ptat and Plpp were relatively strong promoters whilst PldcC and Pspc were relatively weak promoters. Relative promoter strength decreased in the order of Plpp>Ptat>PlysS>PldcC>Pspc during the exponential phase whilst Ptat was stronger than Plpp during the stationary phase of growth. Plpp had its highest level of expression during the exponential phase, while Ptat had relatively stable lux expression during the stationary phase. Correlations between relative bioluminescence and CFU at 24 hours were greater than 0.9 indicating a strong relationship for all bioluminescent strains. Reduction in correlation coefficients to approximately 0.6 between relative bioluminescence and CFU and between relative fluorescence and CFU beyond 24 hours indicated that a certain proportion of cells were viable but non-culturable. Tat-pME-lux showed steady bioluminescence compared to CFU count (R>0.9) throughout 28 days of growth. Equivalence analysis showed no significant difference between the bioluminescence and plate count method throughout 28 days of growth for all five bioluminescent strains. Applicability of these novel bioluminescent strains was evaluated for preservative efficacy tests (PET) using bacterial replication and bioluminescence as a measure of constitutive gene expression. PET using benzalkonium chloride and benzyl alcohol showed no significant difference between the bioluminescent method and the plate count method. Good correlations between bioluminescence, CFU count and fluorescence were obtained for benzalkonium chloride (BKC) concentrations (R>0.9) between 0.0003% and 0.0025% against strains lysR25, lppR4 and tatH5. Similarly, good correlations (R>0.9) between the three parameters were obtained for benzyl alcohol (BA) concentrations between 0.125% and 2% against strains lysR25, lppR4 and tatH5. The bioluminescent method and traditional plate counting method were equivalent for concentrations of BKC (0.0003 - 0.02%) and BA (0.25 - 2%) during preservative efficacy tests. These bioluminescent constructs therefore are good candidates for selection for preservative efficacy testing. The bioluminescent method and traditional plate counting method were also found to be equivalent for construct tatH5 at a concentration of 0.125% BA. PET testing with BKC and BA showed that tatH5-pMElux (R>0.9) had consistently high correlation coefficients between CFU and relative bioluminescence. Together with the results from growth and death kinetics, where tatH5 showed the greatest constitutive expression, it can be concluded that P. aeruginosa ATCC 9027 tatH5-pMElux is the best construct for testing various antimicrobial agents. This study has shown that according to the pharmacopoeial requirements, the bioluminescent method is more accurate, precise and equivalent to the traditional plate counting method and therefore can be utilised instead of the traditional plate counting method for the purpose of preservative efficacy testing.