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Organic matter (OM) is a multi-purpose tool in West African smallholder mixed-farming systems, but its supply has been decreasing for several decades. To assess the viability of a mixed-farming system of south Senegal, carbon (C), nitrogen (N) and phosphorus (P; available in soil and noted POD) budgets (stocks and flows) were thus quantified.The village territory of the study showed a ring-like organisation with growing intensification of fertilization and cropping practices from the periphery (bush ring) to the compounds (compound fields).Stocks in plant and soil averaged 54.7 tC, 2.63 tN and 43.5 kgP ha 1 in old fallows. They were 97, 29 and 251 % higher than in the bush cropped fields, plant biomass accounting for nearly all of the rise. C, N and P amounts recorded in the soil of compound fields were higher than those of the bush field, but the increase was restricted mainly to the 0 10 cm layer. However, the rather weak response of local sandy soils to management can be interpreted only by reassessing the bio-thermodynamical signification of soil organic carbon cycling in the maintenance of the integrity of local agroecosystems.Manageable stocks of the whole village territory were estimated to 29.7 tC, 1.52 tN and 28.6 kgP ha 1 in 1997. Carbon was stored mainly in soil. Livestock, crop harvest and wood collecting were responsible for respectively 59, 27 and 14 % of the C uptake on the village territory. As a result, large C flows were set towards the compound ring (3.8 tC ha 1 y 1). N and P depletion of the system amounted to 4 kgN and 1 kgP ha 1 y 1, suggesting that the system was close to nutrient balance.Under current demographic growth rate, C depletion may reach 0.38 tC ha 1 y 1 and C demand may double during the next three decades. Without any intensification of farming practices, the viability of the system might soon be called into question.

The prevalence of antibiotics on farms for therapeutic and prophylactic use in animals can cause negative effects on biomethane production during anaerobic digestion. Previous literature has found decreased biomethane production rates from a variety of antibiotics, but biogas inhibition differs between studies of continuous and batch reactors and the type of antibiotic studied. Cephalosporin drugs are the most common antibiotic class used to treat mastitis in dairy cows and can retain most of their bioactivity after excretion. Ceftiofur is a commonly used cephalosporin drug but no previous study investigating the effect of Ceftiofur on biomethane during continuous anaerobic digestion has been performed. The aim of this study was to examine the effect on biomethane production when manure from cows treated with Ceftiofur was anaerobically digested. Laboratory sized anaerobic digesters (AD) were run at thermophilic (55°C) temperatures and a 10 day hydraulic retention time. Manure from cows treated with Ceftiofur were fed to the antibiotic treatment reactors for 50 days. The reactor performance was measured by i) biomethane production, ii) waste stabilization in terms of solids and chemical oxygen demand, iii) change in mass of Ceftiofur and iv) change in concentration of antibiotic resistant genes, specifically cfx(A), mef(A), and tet(Q). There was statistically significant decrease in cumulative gas production due to the addition of Ceftiofur into the reactors, but no significant difference between treatments in waste stabilization in terms of percent volatile solids (VS) and total chemical oxygen demand (TCOD) reduction. Anaerobic digestion decreased the amount of Ceftiofur in manure, and the amount of Ceftiofur in the reactors reduced over the time of the experiment. Change in antibiotic resistant genes (ARGs) were gene dependent over time. Concentrations of tet(Q) reduced significantly between feed and effluent of both treatments, and cfx(A) reduced significantly for the control treatment but not the Ceftiofur treatment. Concentrations of mef(A) increased over time in both treatments. Overall, the addition of Ceftiofur in continuously operated anaerobic digesters negatively affected biomethane production, a value-added product responsible for on-farm renewable energy. However, anaerobic digestion does decrease the mass of Ceftiofur within manure, thereby reducing the environmental loading from run-off from farms. / Master of Science / Anaerobic digestion is a biological treatment technology used on farms to treat manure. It can be used to reduce potential environmental damage from contaminants and manure, homogenize manure for fertilizer, and produce methane gas for renewable energy. An emerging challenge in manure management is the presence of antibiotics such as ceftiofur used in animal production to prevent and treat illnesses. When antibiotics are used on livestock, they are excreted from the animal in manure. When the manure is added to the digester, the antibiotic molecules within the manure can kill the bacteria responsible for manure homogenization and gas production. Ceftiofur is a type of cephalosporin antibiotic used to treat dairy cows for mastitis, a bacterial infection of the udder. When the cows are treated with Ceftiofur, it can remain in the excreted manure and enter the digester. The use of antibiotics on farms is also leading to a global phenomenon known as antibiotic resistance. The bacteria that are exposed to antibiotics can develop mutations to become immune to the antibiotic, and can spread the mutations through antibiotic resistant genes (ARGs). ARGs can spread to bacteria which have never been exposed to antibiotics, making them resistant. This causes a significant concern in regards to disease treatment across the world as the efficacy of antibiotics is threatened. Understanding how ARGs move and how they can be eliminated is crucial to preventing global antibiotic resistance. The purpose of this study was to assess the effect of anaerobic digestion on Ceftiofur and ARGs. Four continuous lab-scale anaerobic digesters, two using control manure and two using manure from cows treated with Ceftiofur, were run at 55˚C for a period of 50 days. Over time, the reactor with manure from cows treated with the Ceftiofur antibiotic produced less gas as compared to the control digesters. The amount of Ceftiofur within the digesters decreased over time, demonstrating anaerobic digestion’s ability to degrade the antibiotic molecule. The effect of anaerobic digestion on the ARG concentration was gene specific. The concentration of the tet(Q) gene, a gene responsible for resistance against the very common antibiotic tetracycline, was reduced by anaerobic digestion. These results demonstrate that anaerobic digestion is a technology which can reduce the environmental impact of manure from Ceftiofur-treated cows. This shows that manure treatment can be a first step in combating antibiotic resistance across the globe.

With shifting preferences of consumers towards healthier food, organic food demand has been on the rise for the past two decades. This increased demand has created an opportunity for farmers to shift from conventional to organic production. However, there are risks and uncertainties associated with organic farming. The management of an organic farm in the absence of organic-based disease and pest suppressing strategies constrains farmers from adopting organic vegetable production. The use of cover crops to control soil-borne diseases and suppress weeds and other pests has increased because of its sustainable and environmental friendly nature. This study of the economic impact of the cover crops on organic tomato production in the three states Ohio, New York, and Maryland showed mixed results. In Maryland, mixed forage radish and hairy vetch was projected to have a net present value over 15 years that was $1.53 million higher than single species hairy vetch, assuming maximum adoption level of 50 percent. In New York, mixed rye and turnip gave the higher return with a net present value of $2.61 million. In Ohio, the highest projected return was from mixed hay compared to hairy vetch with a net present value of $3.12 million when used without adding compost amendments. In Maryland and New York when bare ground was also used as a control, only mixed forage radish and hairy vetch in Maryland produced better returns compared to bare ground. A probit regression assessing the factors affecting the decision to adopt mixed species green manure technology indicated that farmer experiences in organic production, farmer age, access to the internet access, and farmers’ perceptions about the benefits of using mixed species green manures were significant factors. Each variables and factors except age had a positive influence. Similarly, probit results for microbial inoculants indicated that education, gender, and access to the internet were significant determinants, and had a negative effect on the probability of adoption. Access to the internet was significant for both mixed species green manures and microbial inoculants but with opposite sign, positive for mixed species green manures and negative for microbial inoculants. / Master of Science

Watershed models are powerful tools for simulating different scenarios to understand the impact of management practices and are used to support and guide decision-making. However, there are often challenges and limitations to using watershed models in some areas of watershed modeling; 1) model calibration in the areas with data limitations; 2) acquiring complete weather data that accurately reflect watershed model responses; 3) accurate representation of manure operation in watershed models. This dissertation addresses each of the aforementioned challenges using new approaches and tools in three studies with the main objective of achieving a better prediction of water quality and quantity and enhancing watershed models. Chapter 2 presents a method (multi-basin calibration (MBC)) to estimate watershed model parameters that lack long-term streamflow records. In the MBC method, first, the Soil and Water Assessment Tool (SWAT) models are initialized individually for several similar neighboring watersheds with a short period of measured streamflow. Then, we aggregate the simulated and observed flows from each initialization with short histories to generate a combined observed-simulated streamflow record that is longer than the initial length of each individual member in order to increase the information content. The Nash-Sutcliffe efficiency (NSE) from this merged time series was used as the basis for calibrating using a differential evolution algorithm. To evaluate the MBC, SWAT models for three newly instrumented USGS gages in Lake Champlain Basin of Vermont, USA, were compared to the commonly used similarity-based regionalization (SBR) approach. Results demonstrate that short periods of hydrological measurement from multiple locations in a basin can represent a system similar to long-term measurements. Chapter 3 develops a method to generate a complete weather data time series with the integration of multiple Global Historical Climatology Network (GHCN) stations and to assess the benefit of much higher density, lower reliability precipitation measurements from private citizens collected by the Community Collaborative Rain, Hail, and Snow (CoCoRaHS) network data that was integrated into the GHCN. To evaluate the performance of the methodology, generated weather data is used to force the Soil and Water Assessment Tool (SWAT) models of 21 United States Department of Agriculture (USDA)-Agricultural Research Service (ARS)-Natural Resource Conservation Service (NRCS)-Conservation Effects Assessment Project (CEAP) watersheds to simulate daily streamflow. The results demonstrated that integration of multiple GHCN stations including higher-density, but perhaps lower-quality weather data can enhance model performance. A comparison with published SWAT model results further corroborated improved model performance using newly combined GHCN data. Chapter 4 develops a hybrid SWAT model, SWAT-Dairy, to accurately represent the impact of manure operation on nutrient transport. The SWAT-Dairy model incorporates process-based livestock routines, developed in the R platform, which quantify daily manure production, stored manure, daily total nitrogen (N) and phosphorus (P), organic and mineral N and P, and dynamic manure nutrient fractions based on animal characteristics, feed characteristics, and environmental conditions. Outputs are then used in SWAT to simulate the impact of livestock manure production. The new model, with simulated manure application management, is applied to a farm in the Little Otter Creek Basin in Vermont, US. Subbasin- and farm-level N and P losses from manure management using the new model were compared for different feed management scenarios. / Doctor of Philosophy / In the past few decades, watershed management has become more challenging due to rapid population growth, climate change, and agricultural practices. In order to achieve better watershed management strategies, it is essential to understand the complex interaction between different biological, physical, and chemical processes occurring in the watershed. Watershed models are useful tools that help scientists and engineers to understand and predict how climate and land-use changes and agricultural management practices affect different components of a watershed system. While watershed models have many advantages, they are often limited by challenges and obstacles, such as model parameter estimations in the areas with limited measured streamflow data, acquiring complete and accurate weather data, and explicit representation of animal management impacts on water quality in manure applications. This dissertation addresses the challenges mentioned earlier by developing new approaches and methods that improve water quality and quantity using watershed models. A long record of measured streamflow data is necessary for watershed models to accurately represent watershed systems and estimate the parameters that cannot be directly measured. However, many watersheds worldwide are not monitored or are newly instrumented with a short period of recorded data. Chapter 2 introduces a new approach (multi basin calibration (MBC)) that integrates short periods of recorded data from several watersheds to provide a similar representation of the watershed system as long-term records. MBC was compared with a commonly used method that requires long recorded streamflow data from a neighboring watershed. The results showed that MBC improved model results and captured hydrological processes better for the watershed with a short period of recorded data than the traditionally used method. Obtaining accurate weather data for a watershed model can also be challenging since land-based weather stations often contain missing data. In recent years, hydrological modelers and researchers have access to the much higher density of weather measurements from private citizens that collect data with inexpensive equipment. However, no study has evaluated the benefits of using much higher-density data from private citizens for watershed modeling. Chapter 3 presents a new methodology to acquire complete weather data time series with the integration of all weather stations, including higher density private citizen-based measurements. Then the weather data were used to force watershed models of 21 watersheds across the United States. The results showed that the new methodology provides weather data that reflect the watershed model response with satisfactory performance ratings in 18 out of 21 watersheds. Lastly, chapter 4 develops a dairy model and integrates it into one of the most commonly used watershed models, the Soil and Water Assessment Tool (SWAT), to investigate how different farm management scenarios impact manure production and nutrient contents as well as their consequent effect on water quality during manure application on farm fields.

京都大学 / 新制・課程博士 / 博士(工学) / 甲第25269号 / 工博第5228号 / 新制||工||1997(附属図書館) / 京都大学大学院工学研究科都市環境工学専攻 / (主査)教授 西村 文武, 教授 米田 稔, 教授 松田 知成 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

Thesis (MScAgric)--University of Stellenbosch, 2001. / ENGLISH ABSTRACT: During Holocene times a considerable deposit of barn owl pellet material accumulated in the Hot Pot Cave at De Hoop Nature Reserve on the southern coast of the Western Cape Province, South Africa. An excavation of this accumulation has yielded information on barn owl prey species over the past some two millennia. Four distinct layers were excavated and radiocarbon-dated to AD 381, AD 615, AD 991 and AD 1417. The micromammalian cranial contents of these layers were compared to material from two pellet collections that represent modem bam owl predation at De Hoop (AD 2000). Comparisons were made from three perspectives: (1) physical size measurements of certain cranial parameters, (2) micromammal community species composition and (3) community structure indices, such as the Shannon-Wiener diversity index, Simpson's diversity index and the species equitability index. By extrapolating from known ecological distribution information of the relevant prey species, these data were used to recreate the local climate at the time of the accumulation of the layers. The results were compared to other palaeoclimate models for the region as a test of validity. It was found that the lower two layers of the sequence represented mild conditions with possibly more grass than in recent times, while the upper layers represented cool weather with a possible increase in scrub. AD 381 was found to be somewhat dry and mild, AD 615 to be the wettest level and possibly milder than AD 381, AD 991 to be the coolest of all the levels and dryest of the ancient levels, AD 1417 to be somewhat cool and probably drier than AD 615, but wetter than AD 381, and AD 2000 to be the mildest and dryest of all levels, with the artificial influence of nearby agricultural activities evident. / AFRIKAANSE OPSOMMING: Tydens die Holoseen tydperk het 'n relatief groot hoeveelheid nonnietjie-uil bolusmateriaal versamel in Hot Pot Grot in die De Hoop Natuurreservaat aan die Wes-Kaapse suidkus, Suid- Afrika. Opgrawings van hierdie bolusversameling het waardevolle en insiggewende inligting aandie lig gebring rakende nonnetjie-uil prooi tydens ongeveer die afgelope tweeduisend jaar. Vier defnitiewe lae is opgegrawe en deur radiodatering is die lae se datums vasgestelop 381, 615, 991 en 1417 n.e. Deur gebruik te maak van kraniale kriteria. is die mikrosoogdier inhoud van die opgrawings vergelyk met dié van twee bolusversamelings wat die huidige uilprooi (2000 n.Ci) in De Hoop verteenwoordig. Die vergelykings is op drie maniere getref: (1) fisiese grootternates van sekere kraniale parameters, (2) species-samestelling van die mikrosoogdiergemeenskap en (3) gemeenskap-struktuur indekse nl. die Shannon-Wiener diversiteitsindeks, Simpson se diversiteitsindeks en die species-gelykheid indeks. Deur ekstrapolasie vanaf bekende ekologiese verspreidingsinligting rakende die betrokke species, is hierdie data gebruik om die klimaat van daardie tydperke te herskep op 'n streeksbasis en vergelyk met ander paleoklimaat-modelle om die geldigheid daarvan te beproef. Die resultate het getoon dat die onderste (oudste) twee lae warmer toestande met moontlik meer gras verteenwoordig, terwyl die boonste twee lae koeler weer met moontlik meer bosse verteenwoordig. Daar is verder gevind dat 381 n.e. redelik droog en warm was, 615 n.e. die natste laag en moontlik warmer as 381 n.e., 991 n.e. die koudste van al die lae en droogste van die grot-lae, 1417 n.e. redelik koel en moontlik droëer as 615 n.e., maar natter as 381 n.e., en 2000 n.C. die warmste en droogste van al die lae, met kunsmatige invloed van nabygeleë landbou aktiwiteite.

<p>Eutrophication was recognized in the early 1900's and results in severe algal blooms, increased biomass production and eventually leads to an altered ecosystem. In Mälardalen we find the most eutrophic lakes. Most of the applied phosphorus and nitrogen into seas and oceans comes from agriculture, and the nutrients losses are affected by local weather conditions and soil type. Guidelines for storage and distribution of manure were introduced in the 80s, and the rules were strengthened January 1, 2010. Localities and previous county administrations can whether there are special reasons, grant an exemption for manure application during winter-periods despite the current ban.</p><p>In this essay I have examined how many exemptions of manure application that has been granted at the Sagån river, and what implications it has for the eutrophication of Lake Mälaren. Sagån’s catchment area extends through Enköping, Sala, Heby, Västerås and Uppsala. Since 2000, 23 waivers of manure application have been granted, most of these deviations have occurred on snow-covered ground or when the weather change considerably. This means that much of the nutriment in the manure went down to the nearby water when the snow melted. Computations were made In order to find out how much phosphorus was spread each time. The conclusion is that the pig manure contains significantly more phosphorus, meaning greater phosphorus losses. However, these calculations are only estimates.</p><p>Several farmers were contacted to find out what they think about the regulation of the management and application of manure. They believe that the new legislation does not pose a problem for them. But farmers with small distribution areas in relation to the number of animals could have some problems.</p>

A integração das espécies de adubos verdes, que imobilizam P, com a aplicação de fontes minerais fosfatadas é uma das medidas agronômicas mais promissoras para melhorar a disponibilidade de P para a cultura principal, com o objetivo de recuperar a fertilidade do solo, aumentar a matéria orgânica do solo e assim, otimizar eficiência de fertilizantes minerais. Os objetivos deste trabalho foram selecionar entre as espécies de adubos verdes mais eficientes em absorver o P na forma menos disponível e pela marcação isotópica destas plantas com ³²P, quantificar o aproveitamento deste nutriente proveniente dos adubos verdes e do fertilizante mineral pela cultura da cana-de-açúcar e do arroz, determinando suas reais contribuições como fonte de P. O estudo foi desenvolvido três fases, em amostras de Latossolo Vermelho Amarelo distrófico típico, com baixo teor de P, em casa-de-vegetação no CENA/USP. O primeiro experimento foi realizado com a finalidade de identificar e selecionar entre as 25 espécies de adubos verdes (AV) mais eficientes em absorver P através de métodos de diluição isotópica. As plantas foram cultivadas em delineamento inteiramente casualizado com três repetições. Com os resultados (13 variáveis) das 25 espécies de adubo verde realizou-se análise estatística de componentes principais (PCA) e de agrupamento hierárquico (AHA) visando grupar os mais similares. A PCA foi explicada pelas duas primeiras componentes, definidas como P acumulado na planta e o valor L, resultando em três grupos, sendo o tremoço a espécies mais eficiente em absorver o P menos disponível, portanto, há diferença entre as espécies quanto à absorção. A partir desses grupos foram selecionadas algumas espécies de adubos verdes para avaliar o aproveitamento do P (AP) do adubo verde e do superfosfato triplo (SFT) pela raiz e parte aérea da cana-de-açúcar (experimento II) e pela parte aérea do arroz (experimento III) através da técnica da diluição isotópica. Para a cana-de-açúcar, foram selecionados braquiária ruziziensis, crotalária juncea, guandu anão, estilosante cv. Campo grande e para o arroz, a crotalária juncea e guandu anão e quatro doses de SFT (0; 30; 60 e 120 mg/vaso de P). Em média, os AVs apresentaram baixo aproveitamento, sendo 0,1 % pela raiz da cana; 0,8% pela parte aérea da cana e 2,0% pelo arroz. Para SFT, o AP pela raiz da cana foi de 1,0%; 9,9% pela parte aérea e 4,0% pelo arroz. Assim, o AP do SFT é superior aos dos adubos verdes para as duas culturas, independente das espécies de adubos verdes. Obtêm-se maior aproveitamento do SFT para ambas as culturas, na dose de 30 mg/vaso de P. O P do adubo verde não é suficiente para suprir a total demanda da cultura principal, mas favorece a absorção do P pela cultura quando associada ao SFT. / Integration of green manures species, which immobilize P, with supplying mineral P sources is one of the most promising agronomic practices for improving the availability of P for the main crop in order to recover soil fertility, increasing soil organic matter and thus optimize the efficiency of mineral fertilizers. The objectives of this work was to select among the green manure species the most efficient in absorbing the less available form of P, and by isotopic labeling of this plants, quantify the use of this nutrient from the green manures and mineral fertilizer by the sugar cane and rice crops, determining their real contributions as a P source. It was developed in three phases, in dystrophic Typical Hapludox, low-P, in the greenhouse in CENA / USP. The first experiment was conducted to identify and select the green manure species the more efficient one in absorbing P by isotopic dilution techniue. The plants were grown in a completely randomized design with three replications. With 25 species of green manures and 13 variables statistical analysis of principal components (PCA) and hierarchical clustering (HCA) were carried out in order to group the most similar ones. The PCA was explained by the first two components, defined as plant accumulated P and the L value, resulting three groups, and the white lupin being the most efficient in absorbing less available P, so there is difference between species on the ability to absorb the less available form of soil P absorption. From these groups some species of green manure were selected to evaluate the P use efficiency (PUE), from green manure and triple superphosphate (TSP), both labeled with ³²P, by sugar cane (experiment II) and rice plant (experiment III) through the isotopic dilution technique. Brachiaria, sunnhemp, dwarf pigeon pea, stylosante cv. campo grande were selected for sugar cane and sunnhemp and dwarf pigeon pea for rice, and four rates of TSP (0, 30, 60 and 120 mg P/ pot). On average, the AVs had low PUE, begin 0.1% by the root of sugar cane, 0.8% by shoot of sugar cane and 2.0% by rice. For TSP, the PUE by sugar cane roots were 1.0%, 9.9% by shoots and 4.0% by rice plants. Thus PUE of TSP is greater than that of green manure for both crops, regardless of green manure species. The highest PUE of TSP for both crops was obtained at rate of 30 mg P/pot. The green manure P is not sufficient to meet total demand for the main crop, but improves the absorption of P by the crop when associated with SFT.