IDL6 and IDL7 are postulated peptides of the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide family in the model organism Arabidopsis thaliana. The genes encoding the postulated peptides, IDL6 and IDL7, were investigated to potentially elucidate the function of the peptides and the possible relations to plant defense and stress tolerance in A. thaliana. A phenotypic characterization study of single and double knockout mutant lines, and over-expression lines of IDL6 and IDL7 was conducted to potentially find a phenotype linked to the genes by analyzing deviations in growth and development, compared to wild type (Wt) A. thaliana. Over-expression lines showed a tendency to have a higher amount of individuals reaching defined growth stages in seedling development, but this could not be concluded to be a phenotype linked to IDL6 or IDL7.The genes have been shown to be highly up-regulated in response to several different stress treatments, both abiotic and biotic in silico data. Several different transgenic lines of A. thaliana were subjected to different types of stress treatments to potentially verify the in silico data. Knockout lines, double knockout lines, over-expression lines and promoter:GUS lines of IDL6 and IDL7 were treated with abiotic stress factors (NaCl, mannitol, UV-B light, H2O2 and paraquat) and biotic stress factors (aphids, Pseudomonas syringae and flagellin22) to investigate differences in tolerance, gene expression and promoter activity in the respective transgenic lines and Wt A. thaliana.No absolute phenotype was detected in experiments related to stress tolerance, and great variations in promoter activity using promoter:GUS lines were observed. However, double knockout mutants of idl6 and idl7 showed a small trend to tolerate NaCl better than Wt A. thaliana and other transgenic lines. The great variation in GUS expression from GUS assay lead to a thorough screening and expression analyses of promoter:GUS lines. Results presented in this work, indicate that expression of the IDL6 and IDL7 genes may be subjected to extensive post transcriptional regulation through mRNA degradation, possibly governed by stress related environmental signals. A novel member of the IDA peptide family, IDL8, was also analyzed. Segregation analyses of knock out lines were conducted and they were genotyped to verify that mutant lines of idl8 were real knockouts. Results presented here show that one of the idl8 mutant lines had the T-DNA inserted to the promoter region of the gene and is postulated to be a real knockout. However, further expression analyses should be conducted to verify that the gene is not transcribed. Over-expression lines of IDL8 were successfully constructed through recombinant DNA technology and by T-DNA insertion using Agrobacterium tumefaciens.

This thesis describes the cytochrome p450 superfamily in the pennate diatom Seminavis robusta. This diatom has a benthic way of life and can adhere to surfaces, and is a new candidate model organism representing the pennate diatoms. The genome of S. robusta is currently being sequenced and is characterized. This revealed that the genome of S. robusta contains 68 genes encoding CYPs, an unusually high number compared to other related organisms. Several unique families and subfamilies were discovered, but also known families from both plants and animals. This reflects the diversity of factors that may have contributed to the evolution of these genes in diatoms, and possibly how a benthic lifestyle may drive this process.Both bioinformatical tools and transcriptional analysis have been used to characterize and explore this superfamily. An experimental study was performed by cultivating S. robusta at different conditions, including normal temperature and light, lower temperature and a day/night cycle. This confirmed some of the CYPs’ involvement in light-/dark-responses, as well as some responses that seemed coupled to temperature.As part of the study, a comparison was made between two software programs used for analyzing gene expression data. This concluded that replacing the software currently used with a new software program with more possibilities will be beneficial for ensuring a higher quality assurance when publishing RT-qPCR data, as well as one step closer publishing according to the MIQE-guidelines.

Soukupová, Z. Burnout Syndrome -comparison of employees and self-employed / en-trepreneurs. Diploma Thesis. Brno: Mendel University in Brno. Faculty of regional de-velopment and international studies, 2016. Thesis supervisor: Mgr. Bc. Jiří Čeněk. This work is focused on Burnout Syndrome among employees and self-employed / en-trepreneurs in public and private sector. There is a comparison of obtained data via online survey which was established based on Maslach Burnout Inventory. The goal is to examine the differences between employees and self-employed / entrepreneurs working in both public and private sector. The work consists of analysis, interpretation of results and discussion together with suggestions.

The combination of Cloud Computing and Business Intelligence (BI) gives many advantages, however the classic deployment model is still in many cases preferred. Both models differ in many aspects and are suitable for various organizations. The thesis looks at these aspects and analyzes the differences that cloud computing brings to the Business Intelligence. Aims of the thesis are to compare both models according the architectural, security, user and financial aspects, influencing of the cloud computing on the implementation of BI and presenting some practical examples of BI solution. To achieve the aims the author uses a literature review, electronic sources and own analysis. Based on the identified impact of the cloud factor the author extends the reference model Management of Business Informatics (MBI).

<p>Studiens syfte är att undersöka hur förekomsten av utbrändhet ser ut</p><p>över olika yrkesområden. Utbrändhet mäts med hjälp av Maslach</p><p>Burnout Inventory General Survey på 1494 slumpmässigt utvalda</p><p>yrkesarbetande personer. En referensgrupp, där personer som blivit</p><p>sjukskrivna pga. stress ingått, användes för att kunna bedöma vilka i</p><p>stickprovet som var kliniskt utbrända. Respondenterna indelades i</p><p>olika yrkesområden utifrån Standard för svensk yrkesklassificering.</p><p>Ytterligare en uppdelning gjordes utifrån kvalifikationsnivån i</p><p>området, samt med hänsyn till grad av kontakt med människor och</p><p>möjlighet att ha kontroll över arbetets utformning. Resultatet visar att</p><p>det inte finns någon skillnad i hur utbrändhet fördelar sig över olika</p><p>yrkesområden. Däremot har en hög kontroll över arbetets utformning</p><p>en positiv påverkan i att sänka graden av utbrändhet.</p> / <p>The purpose of this study is to investigate the occurrence of burnout</p><p>across different occupational areas. Burnout is measured using</p><p>Maslach Burnout Inventory-General Survey on 1494 randomized</p><p>respondents who were a part of the working force. A reference group,</p><p>containing people sick listed due to stress, was used to determine</p><p>clinical burnout. The respondents were divided into different</p><p>occupational areas based on the Swedish Standard Classification of</p><p>Occupations. Further division was done in order to divide the</p><p>respondents according to the qualification level in the area, and also in</p><p>consideration of the extent of human contact and the possibility to</p><p>control the design of ones occupation. The result shows that there is</p><p>no difference in the occurrence of burnout across different</p><p>occupational areas. However it has shown that a high control over the</p><p>design of ones occupation has a positive influence in lower the grade</p><p>of burnout.</p>

Studiens syfte är att undersöka hur förekomsten av utbrändhet ser ut över olika yrkesområden. Utbrändhet mäts med hjälp av Maslach Burnout Inventory General Survey på 1494 slumpmässigt utvalda yrkesarbetande personer. En referensgrupp, där personer som blivit sjukskrivna pga. stress ingått, användes för att kunna bedöma vilka i stickprovet som var kliniskt utbrända. Respondenterna indelades i olika yrkesområden utifrån Standard för svensk yrkesklassificering. Ytterligare en uppdelning gjordes utifrån kvalifikationsnivån i området, samt med hänsyn till grad av kontakt med människor och möjlighet att ha kontroll över arbetets utformning. Resultatet visar att det inte finns någon skillnad i hur utbrändhet fördelar sig över olika yrkesområden. Däremot har en hög kontroll över arbetets utformning en positiv påverkan i att sänka graden av utbrändhet. / The purpose of this study is to investigate the occurrence of burnout across different occupational areas. Burnout is measured using Maslach Burnout Inventory-General Survey on 1494 randomized respondents who were a part of the working force. A reference group, containing people sick listed due to stress, was used to determine clinical burnout. The respondents were divided into different occupational areas based on the Swedish Standard Classification of Occupations. Further division was done in order to divide the respondents according to the qualification level in the area, and also in consideration of the extent of human contact and the possibility to control the design of ones occupation. The result shows that there is no difference in the occurrence of burnout across different occupational areas. However it has shown that a high control over the design of ones occupation has a positive influence in lower the grade of burnout.

The work deals with the implementation of CRM solutions, it's representation in the reference model MBI, which is the main goal of the work. In the first part the market of CRM products is analyzed and the current trends in the industry are introduced. The second part is focused on the aforementioned extension of the reference model MBI based on the knowledge and the experience of professional literature in the implementation of CRM solutions. The extension is realized in the form of four tasks which generalize practices for implementing customer relationship management, further work is completed by factors that describe the practical procedures and instructions for working in CRM solutions. The application used is also defined in this work.

The aim of this thesis is the implementation and integration of solutions for Intelligent Warehouse Management into the ERP system, and its representation in the reference model of MBI. The work is composed of the individual chapters, of which the first chapters provides an extensive search to acquaint the reader with the basic characteristics of logistics, and logistics information technology support, which aims to improve KPI reporting and the level of SCM. The thesis also solves the characteristics and functionality of ERP and WMS systems and systems integration. The main part of the thesis describes the various stages of implementation and integration in the form of four tasks and their related factors representing general practices and procedures for the successful implementation of the system for Intelligent Warehouse Management.

The objective of this diploma thesis is to design a proposal for managing projects in small and mid-sized companies that will be part of reference Model of Business Informatics MBI. MBI is a reference model for business IT, which is being developed by Department of Information Technologies at the University of Economics in Prague. MBI contains best practices in combination with theoretical background. The proposal includes prepared document templates ready to use in project management. This thesis also compares well-known and most widely used project management standards. The theoretical part is focused on the most widely used project management standards and methodologies. First chapter compares PMBOK, ICB and PRINCE2. This chapter is focused on the most important characteristics, used approaches and differences of these standards. Next chapter is closely focused on PRINCE2 described in terms of defined processes. In the practical part of this diploma thesis is a proposal for managing projects in small and mid-sized companies. The proposal takes the form of tables that matches the MBI model structure. This proposal is available on MBI website and is ready to use in practice. The theoretical part of this thesis is mainly based on the official publications and information from official websites of companies focused on project management. Practical part of this thesis is based on theoretical and practical experience. The usability of the results is evaluated in conclusion.

The main objective of this thesis is to design dashboards proposals for MBI metrics. In the theoretical part, the concept of a metric is set in the context of MBI methodology and the concept of dashboard is set in contexts of Business Intelligence and Corporate Performance Management in small and medium-sized enterprises. The theoretical part is resolved by the analysis of information sources. MS Excel PowerPivot tool is used for designing dashboards proposals. Designing dashboards, assessment of the importance of Business Intelligence and Corporate Performance Management in SMEs and the proposed extension of the existing MBI metric classification system are main contributions of the author.