Submitted by KETELIN JULIANE DAL PRÁ null (k.dalpra@hotmail.com) on 2017-01-20T02:22:12Z No. of bitstreams: 1 Dissertação de Mestrado Ketelin J. Dal Prá - FOA UNESP.pdf: 1380407 bytes, checksum: aa6eb3e50dc3c4e8a803bd7b396157fa (MD5) / Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-01-24T12:22:26Z (GMT) No. of bitstreams: 1 dalpra_kj_me_araca.pdf: 1380407 bytes, checksum: aa6eb3e50dc3c4e8a803bd7b396157fa (MD5) / Made available in DSpace on 2017-01-24T12:22:26Z (GMT). No. of bitstreams: 1 dalpra_kj_me_araca.pdf: 1380407 bytes, checksum: aa6eb3e50dc3c4e8a803bd7b396157fa (MD5) Previous issue date: 2016-12-16 / O presente estudo teve como objetivo investigar em glândulas submandibulares de ratos tratados com aguardente de cana, a morfologia, atividade funcional das fosfatases e níveis de mucina. 24 ratos machos e adultos foram divididos em 4 grupos (n=6) de acordo com o tipo de bebida fornecida, aguardente de cana (39º GL) ou água, e ao tempo de tratamento de 75 ou 105 dias. Após os períodos de tratamento, os animais foram submetidos à cirurgia para remoção das glândulas submandibulares, seguido da eutanásia. As glândulas submandibulares do lado direito foram processadas para análise histomorfométrica (Image J) dos ductos estriados, ductos granulosos e ácinos. As glândulas do lado esquerdo foram pesadas e armazenadas a -80 °C, para avaliação da atividade funcional da fosfatase ácida total (FAT), fosfatase ácida resistente ao tartarato (FART), fosfatase alcalina (FAL) e determinação dos níveis de mucina. Para isso foram feitos ensaios bioquímicos por métodos espectrofotométricos. Os dados quantitativos foram submetidos à análise estatística (p<0,05). Os pesos absolutos e relativos das glândulas submandibulares apresentam-se reduzidos em relação aos controles (p<0,05). Na análise histomorfométrica, observamos que houve relevante redução da área dos ácinos (p<0,05) e redução não significativa dos ductos estriados (p>0,05). Nos ductos granulosos ocorreu aumento não significativo da área (p>0,05). As atividades de FAT e FART se apresentaram expressivamente diminuídas nos grupos experimentais (p<0,05), enquanto a atividade funcional de FAL diminuiu de forma moderada (p>0,05). Houve redução significativa dos níveis de mucina pelo efeito do alcoolismo crônico (p<0,05). A partir desses dados foi possível concluir que o alcoolismo crônico, por uso de aguardente de cana afeta a funcionalidade bioquímica e a morfologia da glândula submandibular. / The present study aimed to investigate submandibular glands of rats treated with cane brandy, morphology, functional activity of phosphatases and levels of mucin. 24 male and adult rats were divided into 4 groups (n = 6) according to the type of beverage provided, cane brandy (39° GL) or water, and treatment time of 75 or 105 days. After the treatment periods, the animals were submitted to surgery to remove the submandibular glands, followed by euthanasia. The submandibular glands on the right side were processed for histomorphometric analysis (Image J) of the striated ducts, granular ducts and acini. The left glands were weighed and stored at -80 °C for evaluation of the functional activity of total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), and determination of mucin levels. For this, biochemical tests were carried out by spectrophotometric methods. Quantitative data were submitted to statistical analysis (p <0.05). The absolute and relative weights of the submandibular glands are reduced in relation to the controls (p <0.05). In the histomorphometric analysis, we observed that there was a significant reduction of the acini area (p <0.05) and a non-significant reduction of the striated ducts (p> 0.05). In the granular ducts a not significant increase of the area occurred (p> 0.05). The TAP and TRAP activities were significantly decreased in the experimental groups (p <0.05), while the ALP functional activity decreased moderately (p> 0.05). There was a significant reduction of mucin levels by the effect of chronic alcoholism (p <0.05). From these data it was possible to conclude that chronic alcoholism due to the use of cane brandy affects the biochemical functionality and morphology of the submandibular gland.

Submitted by VANESSA MORAIS BRAITE null (vbraite@fmb.unesp.br) on 2017-09-27T13:56:27Z No. of bitstreams: 1 Dissertação Final.pdf: 994807 bytes, checksum: 8a4fd4046da2b6f8872103545cdfc8c4 (MD5) / Approved for entry into archive by Monique Sasaki (sayumi_sasaki@hotmail.com) on 2017-09-28T14:19:51Z (GMT) No. of bitstreams: 1 braite_vm_me_bot.pdf: 994807 bytes, checksum: 8a4fd4046da2b6f8872103545cdfc8c4 (MD5) / Made available in DSpace on 2017-09-28T14:19:51Z (GMT). No. of bitstreams: 1 braite_vm_me_bot.pdf: 994807 bytes, checksum: 8a4fd4046da2b6f8872103545cdfc8c4 (MD5) Previous issue date: 2017-09-01 / O desenvolvimento de novos dispositivos para monitorar o metabolismo celular e o diagnóstico de doenças expandiu as pesquisas com biossensores, que aliados à nanotecnologia possibilitaram a criação de novos elementos com alta sensibilidade de detecção, especificidade e capacidade de multiplexação, mostrando grande potencial para sua aplicabilidade no diagnóstico clínico. O trabalho foi desenvolvido em duas etapas. A primeira, referiu-se no desenvolvimento de uma metodologia para acoplar o aptâmero conjugado com as nanopartículas de ouro sobre o sensor da Ressonância Plasmônica de Superfície (SPR). Foi utilizado MUA para formação das monocamadas auto-organizadas; ativação dos grupos carboxílicos utilizando solução de EDC/NHS e a imobilização do aptâmero conjugado. Após este processo, foram realizadas as injeções de Mucina Epitelial Polimórfica tipo 1 (MUC1). A segunda etapa, consistiu na mesma metodologia de acoplamento do aptâmero, porém substituindo a MUC1 por sobrenadante da linhagem celular LNCaP (células prostáticas tumorais). Desse modo, foi desenvolvida uma metodologia analítica utilizando aptâmeros e biomarcadores para diagnosticar o Câncer de Próstata (PCa) através da SPR. / The development of new devices to monitor cell metabolism and the diagnosis of diseases has expanded research with biosensors, which together with nanotechnology enable the creation of new elements with high detection sensitivity, specificity and multiplexing capacity. The work has developed in two stages. First, concerning the development of a methodology for the coupling or the conjugate with the gold nanoparticles on the Surface Plasmon Resonance (SPR) sensor. MUA has used for the formation of self-organized monolayers; activation of social media groups, EDC / NHS solution, and a conjugate fit immobilization. After this process, they have performed as injections of Polimorphic Epithelial Mucin type 1 (MUC1). The second step consisted of the same aptamer coupling methodology, but replacing a MUC1 with supernatant of the LNCaP cell line (prostatic tumor cells). Thus, an analytical methodology has developed, using aptamers and biomarkers for the diagnosis of Prostate Cancer (PCa) through the SPR.

A saliva é uma mistura de água, eletrólitos, proteínas e enzimas. A secreção diária normal é de 800-1500ml em adultos. A chamada saliva total, o fluído que realmente está presente na cavidade bucal, é produzida por diferentes glândulas salivares e contém ainda fluído crevicular e elementos transudados do plasma, e derivados da rede capilar da mucosa bucal. É importante entender o papel da saliva na proteção dos tecidos bucais, principalmente porque a co-infecção pode ser um fator importante na ativação e supressão do sistema imune, com papel importante no desenvolvimento e severidade das doenças bucais. Além disso, a cavidade bucal tem um vasto número de microrganismos e antígenos presentes, o que faz com que seja considerada em permanente estado de inflamação, em muitos casos, subclínica. Nosso estudo se propõe a observar a variação da composição salivar frente a presença de alterações locais - gengivites e periodontites. O estudo compara as citocinas salivares TNF-&alpha;, IL-1&beta; e IL-6, e os fatores de defesa, beta defensinas 1 e 2, catelicidina e mucina 2, em três diferentes grupos de pacientes: Grupo 1 (controle) - 40 Pacientes, total ou parcialmente dentados, sem inflamação/infecção bucal; Grupo 2 - 40 Pacientes total ou parcialmente dentados, com sinais clínicos de gengivite; e Grupo 3 - 40 Pacientes total ou parcialmente dentados, com sinais clínicos de periodontite. A presença das citocinas e fatores salivares foram avaliadas por testes ELISA. Foram significativas as alterações encontradas entre os grupos para os diferentes fatores: TNF-&alpha;, e IL-6, beta defensinas 1 e 2, catelicidina e mucina 2. Apenas IL-1&beta; não teve resultados significantes. Assim, indica-se que os componentes salivares possuem importante papel salivar frente à alterações locais. / Saliva is a mixture of water, electrolytes, proteins and enzymes. The daily secretion ranges between 800-1500mL in adults. The called whole saliva is composed by the production of different salivary glands, gingival crevicular fluid, and contain elements transudate from plasma derived from the capillary bed beneath the oral mucosa. It is important to consider the evident and important role of saliva in defense and protection of oral tissues. The effects of co-infecting pathogens have been postulated as an important factor in the activation and/or suppression of immune system, important in many situations, including the severity and rate of disease progression. The oral cavity is continually confronted with a vast number of pathogens and antigens, so, in some way, may be considered an inflammatory environment, although the level of inflammation may be sub-clinical. This study proposed to observe how the presence of local inflammation - gingivitis or periodontitis, may influence the presence of salivary cytokines or defense factors in saliva. The study compared saliva molecular components in three different groups of patients: Group 1 (as control group) - 40 Patients, total or partially dentate, without oral infectious; Group 2 - 40 Patients total or partially dentate, with clinical signs of gingivitis; Group 3 - 40 patients, total or partially dentate, with clinical signs of periodontitis. It checked the presence of TNF-&alpha;, IL-1&beta; and IL-6 cytokines, and defense factors, 1 and 2 beta defensins, cathelicidin and mucin 2. ELISA kits determined the levels of these proteins. Found alterations were significant between groups to TNF-&alpha;, IL-6, 1 and 2 beta defensins, cathelicidin and mucin 2. Only IL-1&beta; had not significant results. Therefore, it indicated that salivary components have important hole related to local situations.

Campylobacter jejuni is a major cause of bacterial gastroenteritis in the developed world; however, many aspects of its biology remain poorly understood, including its colonization of the mucus layer lining the gastrointestinal tract. In this study, we utilized microarray transposon tracking to compile a list of 195 genes essential for the growth of C. jejuni in vitro under microaerophilic conditions. Then we characterized C. jejuni growing in an extracted intestinal mucus medium. We found that C. jejuni will grow efficiently in a medium comprised of either chick and piglet intestinal mucus, and that these media have a dramatic impact on its transcriptome. Within the genes identified as differentially expressed during growth in a mucus medium, we identified a single operon, (cj0481-cj0490), which we have subsequently characterized as being responsible for both the uptake and metabolism of L-fucose. This represents the first observation of carbohydrate metabolism by the otherwise asaccharolytic C. jejuni. We further found that the inability to utilize L-fucose puts C. jejuni at a competitive disadvantage when colonizing the piglet intestine, but not the chick cecum. Finally, we examined C. jejuni’s ability to utilize mucins as a carbon source while growing within the mucus layer. We found that despite mucins being a major source of L-fucose and amino acids within the intestine, C. jejuni has a minimal ability to degrade and utilize mucins on its own. However, close proximity to mucolytic bacteria within the microbiota of the intestine, allows for increased C. jejuni growth. Together, this paints the picture of an organism that is well adapted to survival within the mucus lining of the intestine and establishing itself as part of the intestinal microbiota.

One of the primary objectives of infectious disease research is uncovering the direct link that exists between viral population dynamics and molecular evolution. For RNA viruses in particular, evolution occurs at such a rapid pace that epidemiological processes become ingrained into gene sequences. Conceptually, this link is easy to make: as RNA viruses spread throughout a population, they evolve with each new host infection. However, developing a quantitative understanding of this connection is difficult. Thus, the emerging discipline of phylodynamics is centered on reconciling epidemiology and phylogenetics using genetic analysis. Here, we present two research studies that draw on phylodynamic principles in order to characterize the progression and evolution of the Ebola virus and the human immunodefficiency virus (HIV). In the first study, the interplay between selection and epistasis in the Ebola virus genome is elucidated through the ancestral reconstruction of a critical region in the Ebola virus glycoprotein. Hence, we provide a novel mechanistic account of the structural changes that led up to the 2014 Ebola virus outbreak. The second study applies an approximate Bayesian computation (ABC) approach to the inference of epidemiological parameters. First, we demonstrate the accuracy of this approach with simulated data. Then, we infer the dynamics of the Swiss HIV-1 epidemic, illustrating the applicability of this statistical method to the public health sector. Altogether, this thesis unravels some of the complex dynamics that shape epidemic progression, and provides potential avenues for facilitating viral surveillance efforts.

Les bactéries dans la nature existent principalement en biofilm, qui est une communauté structurée et adhérente de microbes enveloppés dans des matrices polymériques. Dans le corps humain, la plupart de biofilms sont composés de microorganismes commensaux et le tractus gastro-intestinal est le site le plus fortement colonisé. L’attachement bactérien à la couche de gel de mucus couvrant l’épithélium intestinal est fondamental à l’établissement d’une microflore commensale stable. Cependant, les interactions entre les bactéries et le gel de mucus restent mal décrites. En plus, la complexité et la diversité du microbiote intestinal lui-même est un obstacle pour les analyses de son fonctionnement biologique. Les fonctions du microbiote sont le produit de communautés bactériennes complexes, et des interactions entres les différentes espèces qui les composent. De nouvelles approches sont nécessaires pour étudier la génétique de l’espèce la plus étudiée du microbiote de l’intestin humain, Escherichia coli. Cette thèse est consacrée à l’exploration de la réponse transcriptionnelle d’E. coli à différents facteurs présents dans l’intestin humain à travers la réalisation de 3 objectifs principaux. La première partie de mon travail concerne la conception et l’optimisation d’outils génétiques permettant de détecter E. coli au sein de biofilms multi-espèces tout en mesurant simultanément l’activité d’un gène d’intérêt. L’utilisation du gène codant la protéine fluorescente verte (GFP) et de ses dérivés a permis d’importantes avancées sur le marquage des cellules entières ainsi que le suivi d’activité transcriptionnelle. Par contre, l’utilisation de marqueurs fluorescents rouges s’est révélée décevante. Dans un deuxième temps, grâce aux outils mis au point dans la première partie de mon travail, l’influence de la mucine sur la capacité d’E. coli à former des biofilm a pu être étudiée. J’ai montré que la mucine augmente la formation du biofilm d’E. coli par modulation transcriptionnelle de structures d’adhérences telles que les curli et les pili de type 1. Enfin, l’influence de la culture en biofilms multi-espèces constitués d’E. coli et de bactéries commensales (K. pneumoniae and E. faecalis) sur la croissance de chacun des partenaires a été analysée, en focalisant notre attention sur l’influence possible de structures d’adhérence telles que les curli. Les résultats indiquent que la production de curli en biofilm augmente le développement d’E. coli en co-culture avec K. pneumoniae alors qu’elle favorise l’interaction synergique entre E. coli et E. faecalis. Les implications basées sur ces données ont été examinées. Ce travail contribue à l’amélioration des connaissances sur la réponse d’E. coli à l’environnement intestinal et apporte les fondations pour construire des outils plus puissants pour la poursuite des investigations sur les biofilms multi-espèces. / Bacteria in nature mostly exist in biofilms, which are structured adherent communities encased in polymeric matrices. In the human body, most biofilms are composed of commensal microorganisms with the gastrointestinal tract being the most heavily colonized site. Bacterial attachment to the overlying mucus gel layer of the intestinal epithelium is fundamental to the establishment of a stable commensal microflora. However the interaction of bacteria with the complex mucus gel is poorly described. Moreover, the complexity and diversity of the gut microbiota is itself an obstacle to studying its biology. Microbiota functions are the product of communities of bacteria and interactions between multiple species. New approaches are needed to study this aspect of even the most well-studied member of the human gut microbiota, Escherichia coli. This thesis was devoted to the exploration of the transcriptional response of E. coli facing different elements of human gut following 3 main objectives. First, the initial part of my work was related to the conception and optimization of appropriate genetic tools to both track E. coli within the multispecies context that constitute human gut commensals, and survey the expression of genes of interest. Use of the Green Fluorescent Protein (GFP) genes allowing enhanced fluorescence and shortened half-life has permitted significant progress both in whole cell tagging as well as transcriptional reporting, while the red fluorescent counterparts were disappointing. Second, using the subset of tools that has been validated to be reliable, influence of mucin on the biofilm formation ability of E. coli has subsequently been studied. I have shown that mucin promotes E. coli biofilm formation through transcriptional modulation of surface adhesion structures such as curli and type 1 pili. Third, concurrently, E. coli’s population relationship to commensal bacteria (K. pneumoniae and E. faecalis) was investigated and demonstrated, with the possible influence of surface adhesion structures such as curli as the biological focus. The results suggest that curli production in biofilm increases the fitness of E. coli when co-cultured with K. pneumoniae while promoting synergistic interaction between E. coli and E. faecalis. The implication based on the data is discussed. This work improves the understanding of E. coli response to the gut environment, and provides foundations to build more powerful tools for further investigations.

Orientador: Laura Sterian Ward / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-08-07T12:12:10Z (GMT). No. of bitstreams: 1 Morari_ElaineCristina_D.pdf: 1770772 bytes, checksum: f0438710dc4ea98d3ad5dcac857c770e (MD5) Previous issue date: 2006 / Resumo: Como a maior parte dos carcinomas diferenciados tem um excelente prognóstico, alguns autores consideram o tratamento convencional, isto é, a tireoidectomia total seguida de ablação actínica de remanescentes glandulares, excessivo. Combinações de fatores específicos do paciente e do tumor foram propostas para classificação prognóstica, mas na prática são pouco utilizadas. Os resultados de alguns trabalhos sugerem relação entre a expressão dos genes MUC, p53 e NIS e a evolução clínica das neoplasias tireoidianas. Com o objetivo de investigar a utilidade clínica de marcadores imonoistoquímicos de prognóstico, estudamos 67 nódulos tireoidianos, incluindo 57 carcinomas, dos quais incluiu 34 (50,74%) carcinomas papilíferos, sendo 21 (31,3%) casos do tipo histológico clássico, 7 (10,4%) da variante de células altas e 6 (9%) da variante folicular. Avaliamos também 16 (23,9%) carcinomas foliculares, 4 (6%) medulares e 3 carcinomas anaplásicos (4,5%). Dez casos (14,9%) foram obtidos de pacientes submetidos à cirurgia com o diagnóstico de doenças benignas da tiróide (3 casos de bócio nodular, 4 adenomas foliculares e 3 tecidos tireoidianos normais). Todos os pacientes foram submetidos a um mesmo protocolo de tratamento e acompanhados por 66±28 meses (mediana de 63 meses) sendo classificados como apresentando evolução favorável ou desfavorável. Consideramos de evolução desfavorável os 19 pacientes que possuíam evidência de recorrência local ou à distância ou que foram a óbito durante o seguimento pela doença. Realizamos estudos imunoistoquímicos da detecção das proteínas NIS, p53 e MUC1 comparando-os com a evolução dos pacientes. Mostramos que pacientes com carcinomas bem diferenciados, ausência de metástases, estadio II e estadiamento T2 têm uma evolução melhor em relação aos pacientes com carcinomas pouco diferenciados, presença de metástases, estadio IV e estadiamento T4. Não encontramos relação entre a expressão das proteínas NIS, p53 e MUC1 e a evolução dos pacientes estudados / Abstract: Because most differentiated thyroid carcinomas have an excellent prognosis, some authors have been claiming that these patients have been over-treated. Combinations of patient-and tumor-specific factors have been proposed for prognostic stratification, but no clinicpathologic staging was demonstrated to be useful at the present time. In order to investigate the clinical utility of immunohistochemistry markers, we studied 67 thyroid nodules including 50 thyroid carcinomas. There were 34 (50,74%) papillary carcinomas, including 21 (31,3%) cases of the classical histological type, 7 (10,4%) tall cell variants and 6 (9%) of the follicular variant type. Also, there were 16 (23,9%) follicular carcinomas, 4 (6%) medullary and 3 anaplastic carcinomas (4,5%). Ten patients (14,9%) were submitted to surgery because of benign thyroid diseases (3 nodular goiter cases, 4 follicular adenomas and 3 thyroid tissues normal). All patients were submitted to a similar management protocol and followed-up for 66±28 months (median of 63 months) and classified as presenting a good or a bad evolution. We considered as presenting a bad evolution 19 patients that presented evidence of local or distant recurrence and the patients that died during the follow-up. Immunohistochemical expression of NIS, p53 and MUC1 proteins was compared to patient¿s evolution. We showed that patient with well differentiated thyroid carcinomas, absence of distant metastases, stage II and staging T2 have a better evolution in relation to the patients with poorly differentiated thyroid carcinomas, presence of metastases, stage IV and staging T4. We were able to find relation between the expression of proteins NIS, p53 and MUC1 and prognostic of the studied patients / Doutorado / Clinica Medica / Doutor em Clínica Médica

The scope of this masters thesis is the preparation of a model mucin system and its utilization as an artificial model of the native mucus system. The creation of this model system, according to several designed methods was a part of experimental part of the thesis. The preparation of mucin system comprised of physical and chemical methods of hydrogel formation, screening and characterisation of the various physical conditions of the mucin properties on its molecular level, and the preparation of sorbent with sorption surface containing mucin. Methods of light scattering, namely dynamic light scattering (DLS), used for mucin particles size change monitoring, and electroforetic light scattering (ELS), used for Zeta potential change monitoring, were used for the screening of the impact of physical factors on the properties of mucin.For the characterisation of impact of the temperature on changes in mucin sctructure was, apart from monitoring of light scattering, used also a diferential scanning calorimetry (DSC), which registered temperature value, at which mucin thermal denaturation occurs. In the next part of the thesis we subdued the created sorption surfaces to various physical-chemical analyses, which task is the characterisation and projection of surface and confirmation of mucin presence.Substancial part in monitoring and characterisation of changes in surface sctructure of sorption surface was accomplished by Fourier transform infrared spectroscopy (FTIR). Scanning electron microscophy (SEM) was used for the final, more detailed, projection of the mucin enriched, sorbent surface structure. Suggested methods of mucin hydrogel, didnt prove sufficient results for the possibility of application of hydrogel as a artificial model of real mucus system, but the sorbent application was indicated as a suitable alternative and an instrument for the further mucin behaviour research and possibly subsequent bacterial adhesion, which represents the first step in the formation of the bacterial biofilm.

CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical technology Name and surname: Marie Korpasová Title of diploma thesis: Adhesive and rheological properties of mixtures based on chitosan Supervisor: PharmDr. Eva Šnejdrová, Ph.D. Diploma thesis deals with evaluation of rheological and adhesive properties of compounds for matrix tablets formulation, based on Chitosan with addition of retardant compound. The retardant compound consists of Sodium Alginate and Hypromellose in concentrations of 30 %, 40 % and 50 %. Theoretical part describes Chitosan, Sodium Alginate and Hypromellose. As next is division of matrix tablets. Theoretically is also described evaluation of rotational, oscillational and adhesive testing which was used in practical part. From viscosity curves can be seen, that viscosity is decreasing with increasing stress. Viscosity increases with increasing concentration of Sodium Alginate and Hypromellose. Oscillational testing is better for gel characterization, because it provides lower stress on test samples. Samples of given compound were prepared by hydrating with phosphate buffer of pH 6,8. Coefficient of consistency K and index flow rate n characterize rheological behaviour of gels. Viscoelastic compounds behave like solids with plastic...

CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Name of author: Dominik Vavřich Title of diploma thesis: Rheological behaviour of mixtures for freeze-drying Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The diploma thesis deals with the evaluation of rheological properties of aqueous dispersions of fish gelatin and carrageenans intended for lyophilization. Theoretical part of this work is dedicated to the characterization of fish gelatin and carrageenans which were used for preparation of the mixtures in experimental part of this work. Characterization of an oral cavity from an application of medicaments point of view and an orally disintegrating tablets of the medicine with a focus on the lyophilized tablets are also presented. Mixtures of the fish gelatin and the carrageenans were prepared in the experimental part of the work. Their rheological behaviour employing an absolute rotational rheometer Kinexus Pro+ was measured and evaluated. Also, the testing of the rheological characteristics of these materials transformed into the freeze-dried tablets was performed. Nonlinear viscous curves were obtained, and they were mathematically evaluated by the Power law model. Pseudoplastic behaviour of the mixtures can be derived from the results of acquired...