O objetivo do presente estudo foi analisar o preenchimento de canais radiculares após a obturação com sistema RealSeal®, em relação à técnica de condensação lateral, do cone único e termoplastificada, por meio do software Leica Qwin. Para tanto, vinte e sete incisivos centrais inferiores foram preparados com limas rotatórias K 3Endo, hipoclorito de sódio 1,0%, pH 11 associado a Endo PTC e irrigação final relaizada com EDTA 17%. Para a obturação, dividiu-se os espécimes em três grupos, de acordo com a técnica: Grupo I (condensação lateral), Grupo II (cone único), Grupo III (termoplastificada). Após a obturação dos dentes, os mesmos permaneceram em 100% de umidade e 37 graus C, durante 7 dias. Depois disso, os dentes foram incluídos em resina ortoftálica e os blocos de resina contendo os espécimes passaram por micrótomo de tecidos duros, obtendo-se secções tranversais do terço apical, médio e cervical dos canais obturados. Os cortes foram analisados em estereomicroscópio em aumento de 40 vezes, e as imagens obtidas avaliadas no programa Leica Qwin, determinando a porcentagem de área de cimento, material principal e falhas. Os resultados obtidos foram submetidos a análise estatística pelo teste Kruskal-Wallis que determinou diferença estatística ao nível de 5%. Houve diferença entre as técnicas obturadoras, sendo a técnica de cone único a que apresentou maior porcentagem de cimento obturador nos três terços; a termoplastificada apresentou a maior porcentagem de Resilon, mas no terço médio apresentou a maior porcentagem de falhas. / The aim of this study was to compare the proportion of core material, endodontic sealer and voids in the lateral condensation, single cone and thermoplastic techniques, through software Leica QWin. Twenty-seven central mandibular incisors were prepared using K3 Endo with sodium hypochlorite 1,0%, pH 11, Endo-PTC and EDTA 17% for final rising. The roots were, thereafter, randomly assigned into three groups according the filling technique: Group I (lateral condensation), Group II (single cone) and Group III (thermoplastic) and RealSeal® system. After this, the roots were stored for seven days at 37 grays Celsius and 100% relative humidity. Specimens were then embedded in resin and cross-sections were obtained by cervical, middle and apical thirds using a microtome. The samples were analyzed in stereomicroscopy at a magnification of 40 times and the digital image were taken and transferred to software Leica QWin. This software was used to calculate the cross-section area of Resilon, sealer and voids. The results were compared statistically by Kruskall-Wallis test and showed 5% significant difference. The single cone was the most percentage of sealer, thermoplastic was the most of Resilon, but with the most percentage of voids in middle third.

FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior / O propÃsito deste estudo in vitro foi comparar a qualidade do selamento apical de canais radiculares reobturados com o sistema Resilon/Epiphany, quando inicialmente obturados com a guta-percha em associaÃÃo a dois cimentos endodÃnticos (Endofill e AH-Plus), bem como com prÃprio sistema Resilon/Epiphany, apÃs duas tÃcnicas de desobturaÃÃo. Para a avaliaÃÃo da infiltraÃÃo apical, cem raÃzes de dentes humanos recÃm-extraÃdos foram instrumentadas e divididas aleatoriamente em trÃs grupos experimentais de acordo com o material escolhido para a primeira obturaÃÃo (Guta-Percha/ Endofill, Guta-percha/AH-Plus e Resilon/Epiphany) e dois grupos controle, positivo e negativo. ApÃs sete dias acondicionados em estufa a 37 C e 100% de umidade, cada grupo experimental foi aleatoriamente subdividido em dois subgrupos de acordo com a tÃcnica de desobturaÃÃo (instrumentaÃÃo rotatÃria associada ao clorofÃrmio e instrumentaÃÃo rotatÃria sem clorofÃrmio), totalizando seis subgrupos experimentais. Em seguida, as raÃzes foram reinstrumentadas e reobturadas com o sistema Resilon/Epiphany. ApÃs mais sete dias em estufa, sob as mesmas condiÃÃes, as raÃzes foram imersas em tinta Nanquim, centrifugadas e diafanizadas. A medida linear de infiltraÃÃo apical do corante e da quantidade de material obturador residual foi obtida atravÃs do programa de mediÃÃo de imagens NIH ImageJ. Foram realizados os testes Anova e Tukey para comparaÃÃo das medidas de infiltraÃÃo e o teste nÃo-paramÃtrico de Kruskal Wallis para a comparaÃÃo das medidas de resÃduo nos trÃs terÃos radiculares e entre os materiais utilizados. O valor de p<0,05 foi considerado estatisticamente significante. O subgrupo 1(Endofill/desobturaÃÃo sem solvente) apresentou os piores resultados de infiltraÃÃo jà o subgrupo 6(Resilon/Epiphany/desobturaÃÃo com solvente) apresentou as menores medidas de infiltraÃÃo pelo corante. NÃo houve diferenÃa na comparaÃÃo do resÃduo entre os trÃs terÃos (p=0,226), mas houve diferenÃa estatisticamente significante quando se comparou os grupos obturados com Resilon/Epiphany com e sem solvente. ConclusÃo: NÃo houve diferenÃa quanto à presenÃa de resÃduo nos trÃs terÃos radiculares nas duas tÃcnicas utilizadas, mas o grupo obturado com Resilon/Epiphany apresentou a maior quantidade de resÃduo apÃs sua desobturaÃÃo sem solvente, jà os canais obturados e reobturados com Resilon/Epiphany, com a utilizaÃÃo do solvente na desobturaÃÃo, apresentaram os menores Ãndices de infiltraÃÃo apical quando comparado com os demais. / The purpose of this in vitro study was to compare the quality of apical sealing of refilled root canals using the Resilon/Epiphany system, when previously filled with Gutta-percha in association with two endodontics sealers (Endofill and AH-Plus), as well as compare the same quality sealing using the Resilon/Epiphany system itself, after use of two techniques of material removal. To evaluate the apical leakage, one hundred roots of human teeth were extracted, instrumented and randomly divided into three experimental groups, according to the material chosen for the first filling (Gutta-Percha/Endofill, Gutta-Percha/AH-Plus and Resilon/ Epiphany) and two control groups, positive and negative. After seven days in an oven (37ÂC, humidity of 100%), each experimental group was randomly subdivided into two subgroups according to the material removal technique (rotary instrumentation associated with chloroform and rotary instrumentation with no chloroform), leading six experimental subgroups. After this, the roots were instrumented again and refilled using Resilon/Epiphany system. After a week, under the same conditions in an oven, the roots were immersed in India ink, centrifuged and cleared. The India ink apical leakage linear measurement, and remaining filling debris on the root canal walls were assessed by NIH ImageJ program. The ANOVA and Tukey tests were used to make the comparison of the apical measurement leakage and the non parametric Kruskal Wallis test to compare the remaining filling debris in the three analyzed thirds and among the material used. The value p<0,05 was considered to be statistically significant. With regard of the presence of leakage, the subgroup 1(Endofill/removal filling technique without solvent) presented the worse linear apical leakage values. However, the subgroup 6(Resilon/Epiphany/removal filling technique with solvent) presented the lowest leakage measurement values. There was no difference between the remaining debris measurements in the three radicular thirds analyzed (p=0,226), but the subgroup filled with Resilon/Epiphany presented the higher amount of debris after the filling material removal without solvent, as well as, roots canals filled and refilled with Resilon/Epiphany, using chloroform, presented the lowest linear apical leakage values when compared with the others.

Orientadores: José Flávio Affonso de Almeida, Caio Cezar Randi Ferraz / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-23T00:09:35Z (GMT). No. of bitstreams: 1 Pantoja_CarlosAugustodeMoraisSouto_D.pdf: 16994021 bytes, checksum: 70afce610b0b38f5755e1abfe21c858f (MD5) Previous issue date: 2013 / Resumo: Este trabalho foi dividido em 2 capítulos, sendo que no capítulo 1 o objetivo foi de avaliar a influência do etanol na rugosidade, na energia livre de superfície da dentina radicular e na interação entre o cimento AH Plus e a superfície dentinária. Foram selecionadas 100 raízes de dentes humanos anteriores superiores e padronizadas com comprimento de 18 mm. As raízes foram cortadas longitudinalmente, obtendo-se 200 amostras dentinárias, divididas aleatoriamente em 4 grupos (n = 50), de acordo com os métodos de secagem: (1) úmido: apenas aplicação de ponta aspiradora, (2) pontas de papel absorvente: ponta aspiradora + pontas de papel absorventes, (3) solução de etanol 70%: etanol 70% (1 minuto) + ponta aspiradora + pontas de papel absorvente, (4) solução de etanol 100%: etanol 100% (1 minuto) + ponta aspiradora + pontas de papel absorvente. As amostras dentinárias foram avaliadas com o auxílio do rugosímetro e goniômetro, dentro dos seguintes parâmetros de avaliação: (1) rugosidade superficial; (2) energia livre de superfície e (3) ângulo de contato formado entre o cimento AH Plus e superfície dentinária. No capítulo 2, o objetivo foi avaliar a influência do etanol no preenchimento dos túbulos dentinários. Foram selecionadas 40 raízes de dentes humanos anteriores superiores, com comprimento radicular padronizado em 18 mm, e divididas aleatoriamente nos mesmos 4 grupos descritos anteriormente. Após a instrumentação, as raízes foram obturadas pela técnica de Schilder, com guta-percha e cimento AH Plus misturado com o marcador fluorescente Rodamina B. Para a visualização em microscopia confocal de varredura a laser foram confeccionadas 4 secções transversais (1 mm de espessura), em cada terço radicular. Foram avaliados os seguintes parâmetros: (1) porcentagem do perímetro da parede do canal coberta com cimento; (2) máxima profundidade de penetração do cimento endodôntico no interior dos túbulos dentinários; (3) porcentagem da área da dentina penetrada pelo cimento e (4) valor da intensidade do marcador fluorescente Rodamina B. Os resultados encontrados foram analisados estatisticamente pelos testes ANOVA / Tukey, com nível de significância de 5%, em ambos os capítulos. No capítulo 1 observou-se que nos grupos em que se utilizou o etanol, quando comparados aos demais, foram encontrados menor rugosidade de superfície da dentina, maior energia livre de superfície e menor ângulo de contato formado entre o cimento AH Plus e a superfície dentinária. Já no capítulo 2, não foi verificada diferença significante entre os grupos, quanto à avaliação da porcentagem do perímetro da parede do canal coberta por cimento. Entretanto, maiores valores de profundidade de penetração e da porcentagem da área da dentina penetrada pelo cimento foram encontrados no Grupo etanol 100%, seguidos pelos Grupos etanol 70%, pontas de papel e úmido. O etanol 100% atingiu resultados significativamente maiores que a concentração 70%, exceto para a intensidade da Rodamina B no terço cervical. Além disso, os resultados do Grupo pontas de papel foram melhores que o Grupo úmido, com exceção da intensidade da Rodamina B no terço apical. Diante da análise dos resultados apresentados nos 2 capítulos, verificou-se que o etanol (70% e 100%) proporcionou melhor molhabilidade do cimento AH Plus na superfície dentinária. Da mesma forma, as soluções de etanol, especialmente na concentração de 100%, favoreceram a penetração do cimento endodôntico no interior dos túbulos dentinários / Abstract: This study was divided into 2 chapters. In chapter 1, the aim was to evaluate the influence of ethanol on dentin roughness, dentin surface free energy and interaction between dentin surface. A hundred roots of human maxillary teeth were selected with length standardized in 18 mm. The roots were split longitudinally yielding 200 dentin samples, which were divided randomly into 4 groups according to the drying method used: (1) wet: vaccum only (2) paper points: vaccum + absorbent paper points, (3) 70% alcohol: 70% alcohol (1min) + vaccum + absorbent paper points and (4) 100% alcohol group: 100% alcohol (1min) + vaccum + absorbent paper points. The specimens were measured with aid of goniometer and rugosimeter within the following evaluation parameters: (1) surface roughness, (2) surface free energy and (3) contact angle formed between AH Plus sealer and dentin surface. In Chapter 2, the aim was to evaluate the influence of ethanol on dentinal tubules filling. Forty roots of human maxillary teeth with length standardized in 18 mm were selected. The roots were randomly divided among the same 4 groups described above. After instrumentation, the roots were filled by Schilder's technique with gutta-percha and AH Plus mixed with rhodamine B fluorescent marker. The roots were cross-sectioned and dentin sections (1 mm thick) were analyzed by confocal laser scanning microscopy. The evaluation parameters were: (1) Percentage of root canal wall coverage, (2) maximum depth of sealer penetration into dentinal tubules, (3) percentage of penetrated area and (4) fluorescent rhodamine B intensity. The results were statistically analyzed by ANOVA and Tukey tests (?=5%). In Chapter 1 the groups in which ethanol was used lower dentin surface roughness, higher surface free energy and lower contact angle formed between AH Plus and dentin surface were found when compared to the control groups. In chapter 2, there was no significant difference between the groups regarding the percentage of root canal wall coverage. Higher values of maximum depth of sealer penetration into dentinal tubules and percentage of penetrated area were found in Group 100% ethanol, followed by 70% ethanol, paper points and wet groups. The results achieved by 100% ethanol were significantly higher than 70% ethanol, except for intensity of rhodamine B in cervical third. Furthermore, the paper points results were better than wet group, except for intensity of rhodamine B in apical third. After analyzing the results presented in the two chapters, it was verified that ethanol solutions (70% and 100%) provide better wettability of AH Plus sealer on dentin surface. Similarly, ethanol solutions, especially at 100% concentration, improve the penetration of the sealer into dentinal tubules / Doutorado / Endodontia / Doutor em Clínica Odontológica

Orientador: Francisco Jose de Souza Filho / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-04T11:46:55Z (GMT). No. of bitstreams: 1 SouzaFilho_AdelmoMoraesde_D.pdf: 952695 bytes, checksum: b963a86dc214923748fe9bccb4881a3f (MD5) Previous issue date: 2005 / Resumo: Este estudo retrospectivo avaliou a porcentagem de sucesso/insucesso de 76 (setenta e seis) casos de cirurgia parendodôntica com o emprego de técnicas microcirúrgicas e retrobturação com o ionômero resinoso Geristore como material retrobturador, e operados por um único cirurgião. O tempo de observação variou entre um e quatro anos. O critério de avaliação radiográfica foi o de Molven et al. (1987), complementado por avaliações clínicas. Os casos foram considerados sucesso quando classificados como reparo completo ou reparo incompleto (cicatriz fibrosa) e o exame clínico não indicava qualquer sinal ou sintoma de inflamação. Os dados obtidos foram avaliados estatisticamente pelo teste exato de Fisher e a concordância entre os avaliadores verificada pelo teste de Kappa. Os seguintes fatores relevantes em relação ao sucesso foram analisados: indicação cirúrgica, presença de retratamento endodôntico, grupo dental e tempo de avaliação. O índice geral de sucesso foi de 92,2%. Dos fatores analisados, pôde-se observar que houve estabilidade dos resultados considerados como sucesso; não houve diferença estatisticamente significativa entre os índices de sucesso obtido em raízes retratadas endodônticamente e as não retratadas; não houve diferença estatisticamente significativa entre os índices de sucesso obtidos em casos de reoperação e casos operados pela primeira vez e não houve diferença estatisticamente significativa entre os grupos dentais analisados. Tendo em vista o alto índice de sucesso e estabilidade dos resultados obtidos, pôde-se concluir que a adoção de critérios rigorosos de indicação cirúrgica, o emprego do ionômero resinoso Geristore como material retrobturador em conjunto com técnicas microcirúrgicas irão resultar em alto índice de sucesso nas cirurgias parendodônticas, sendo uma boa alternativa para esta modalidade cirúrgica / Abstract: Retrospective observations were made on the success rate of endodontic surgery with microsurgical techniques using the resin ionomer Geristore as root-end filling material. Seventy-six cases were treated by one surgeon between 1999 and 2003. For inclusion in this retrospective study, a follow-up examination at 12 months minimum was required. The radiographic findings were evaluated separately by two independent clinicians using the evaluative criteria of Molven et al. (1987). Radiographic diagnosis was complemented by clinical evaluations in which the presence or absence of signs and symptoms indicative of inflamation were observed. Cases were considered successful when the radiographic assessment showed complete healing or incomplete healing (scar tissue) and the absence of signs or symptoms. The data obtained by these evaluations were submitted to Fisher¿s exact test and the agreement between the observers verified by Kappa¿s test. The following factors were analyzed: surgical indication, status of endodontic re-treatment, tooth type, and observation period. The final success rate was 92.2%. The results of cases classified as successful were considered stable, there was no statistically significant difference between endodontically re-treated and non re-treated teeth, there was no statistically significant difference between the success rates of cases subject to re-surgery and cases surgically treated for the first time; there was no statistically significant difference among the analyzed tooth types. The high success rate and the stability of successful cases observed in this study suggest that the combination of microsurgical techniques, the observance of strict indication criteria and the use of the resin ionomer Geristore as retrofilling material will result in predictable prognosis and in a high success rate of apical surgery / Doutorado / Endodontia / Doutor em Clínica Odontológica

After cleaning and shaping of the root canal the final objective of the endodontic procedure is to obtain a three-dimensional obturation of the root canal space with a fluid-tight seal at the apical foramen. The objective of this in vitro study was to evaluate four different obturation techniques in respect of: • the radiographic quality of root canal obturation, • apical leakage and • the potential of these techniques to obdurate lateral canals One hundred and sixty canals were prepared by using RaCe nickel titanium rotary files to a size 30 with 6% taper. During preparation irrigation was done with TopClear Solution (17% EDTA and 0.2% cetremide) and ChlorXTRA (6% sodium hypochlorite). The canals were divided in four groups of forty canals each and were obturated using the Hybrid Root SEAL technique, the EndoREZ technique, the System B/Obtura technique and the Thermafil technique. The Radiographic Quality of Root Canal Obturation: Digital radiographs were taken of the four groups of obturated canals from a buccolingual and a mesiodistal direction. The quality of obturation was determined for the coronal and apical halves of each canal and scored according to radiographic appearances. The data was tabulated and statistically analyzed using the Mann- Whitney U test. The Hybrid Root SEAL technique demonstrated a statistically significant higher number of radiographic defects in the coronal aspects of the root canals when compared to the System B/Obtura and Thermafil techniques (p<0.05). There was no statistically significant difference between the radiographic defects in the coronal aspects of the root canals between Hybrid Root SEAL and EndoREZ techniques (p>0.05). The Hybrid Root SEAL technique demonstrated a statistically significantly higher number of radiographic defects in the apical aspects of the root canals compared to all the other groups (p<0.05). Apical Leakage: Twenty obturated canals of each of the four groups were processed for evaluation of apical leakage. The root surfaces were coated with nail varnish and sticky wax, leaving 4.0 mm around the apical foramen exposed. Specimens were immersed in 2% methylene blue dye for 48 hours, rinsed in distilled water and embedded in clear acrylic resin. Specimens were sectioned horizontally in 1 mm increments and the extent of dye penetration was measured to the nearest millimeter using a stereomicroscope. The data was tabulated and statistically analyzed using the Man-Whitney U test. The specimens that were obturated with the EndoREZ technique demonstrated the least apical leakage compared to all the other groups tested in this study. However, there was only a statistically significant difference when the EndoREZ technique was compared to the Hybrid Root SEAL and System B/Obtura techniques (p<0.05). The specimens that were obturated with the System B/Obtura technique demonstrated the most apical leakage compared to all the other groups tested in this study. However, there was only a statistically significant difference when the System B/Obtura technique was compared to the EndoREZ and Thermafil techniques (p<0.05). The Potential to Seal Lateral Canals: Twenty obturated canals of each of the four groups were processed for evaluation of the potential to seal lateral canals. The specimens were subjected to a clearing technique and a morphological analysis was performed using a stereomicroscope. Lateral canals were counted and graded within the coronal, middle and apical thirds of the roots. The data was tabulated and statistically analyzed using the Man-Whitney U test. The Thermafil technique demonstrated the greatest number of filled lateral canals. However, there was no statistically significant difference between the Thermafil technique and all the other techniques (p<0.05). / Dissertation (MSc)--University of Pretoria, 2009. / Odontology / unrestricted

Magister Chirurgiae Dentium (MChD) / The goal of root canal treatment is to eradicate microorganisms in the root canal system of the tooth. However; it has been found that no method of tooth preparation is efficient in eliminating all microorganisms present in root canals. Therefore, obturation materials with anti-microbial properties are advantageous, so that any residual microorganisms in the root system of the tooth can be eliminated. Therefore, the aim of the study was to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and Guttaflow bioseal™ against Enterococcus faecalis. The Direct Contact test was used to assess the antibacterial effect of the 3 sealers against E. Faecalis. Sample size was n=50 per sealer. The survival of bacteria was assessed by culturing aliquots of 100 μL onto Tryptic Soy Agar plates after 10-fold serial dilutions. After incubation for 24 hours at 37⁰C, colonies on the plates were counted, and the CFU/mL was calculated. The experiments were performed in triplicates. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7 days, 14 days, 21 days and 28 days. All 3 sealers displayed evidence of antibacterial activity against E. Faecalis with various degrees of antibacterial activity at day 0, 7, 14, 21 and 28. Antibacterial activity was displayed by all 3 sealers against E. Faecalis which will have an effect on entombed bacteria.

Depuis plusieurs années, la Haute Autorité de Santé favorise la diffusion des démarches d'évaluation des pratiques des professionnels de santé. L'idée est de faire progresser la qualité et la sécurité des traitements endodontiques réalisés dans un Centre de Soins et de traitements dentaires en analysant le résultat des traitements réalisés par rapport à des recommandations de bonnes pratiques, puis en mettant en oeuvre des actions d'amélioration. L'évaluation du traitement endodontique a fait l'objet de nombreuses études publiées au cours de ces dernières années. Les résultats hétérogènes rapportés reflètent des différences considérables entre les études concernant notamment l'échantillonnage (type et nombre de dents composant l'échantillon, sélection des cas cliniques), le traitement (procédures, techniques, restauration), et la méthodologie utilisée (conception de l'étude, interprétation radiographique, période de suivi, analyses, mesures des résultats, et critères de succès/échec. Ce travail vise à définir les critères de qualité d'un traitement endodontique afin d'évaluer si les traitements endodontiques réalisés dans le service d'odontologie du CHU de Clermont satisfont ces critères. Une revue de la littérature permet d'établir que 3 critères relevés sur les radiographies postopératoires permettent de décrire le résultat du traitement endodontique. La longueur de l'obturation, sa densité et le fait que l'ensemble du réseau canalaire est concerné par le traitement peuvent être associés en un indicateur mixte qui reflète avant tout le résultat de la phase instrumentale de la procédure. Dans une deuxième partie, cet indicateur composite a été utilisé dans une étude rétrospective des traitements endodontiques réalisés par les étudiants de 4ème et 5ème année au CHU de Clermont-Ferrand. Les critères de qualité technique des traitements impliquaient : 1°) que la limite de l'obturation soit située dans les 2 mm apicaux (pour toutes les racines) ; 2°) que l'image de l'obturation reflète soit dense, homogène et n'incluant pas de vides ; 3°) que tous les canaux visibles et/ou attendus soient obturés. Cette étude rapporte un taux de succès de 58%, valeur relativement haute, par rapport aux études similaires qui varient de 26 % à 57%. Ce pourcentage est dépendant du type de dent, de son état pulpaire, mais ne dépend pas ni de l'expérience de l'étudiant, ni de l'état périapicale. Cet indicateur a également été utilisé dans une deuxième étude qui est la première à fournir des données qualitatives et quantitatives sur les traitements endodontiques réalisés sous anesthésie générale. La qualité technique des traitements sous anesthésie générale est équivalente à celle qui est acceptée par des critères académiques dans les services hospitalo-universitaires. Cette première analyse encourage la réalisation de soins endodontiques sous anesthésie générale et fait la promotion des indications d'anesthésie générale chez l'adulte à des fins de soins dentaires conservateurs. Le pourcentage de traitements endodontiques réalisés dans le respect des recommandations professionnelles de la HAS et satisfaisant les standards académiques des traitements endodontiques constitue un indicateur de qualité des soins. Les deux études qui supportent cette thèse révèlent que les valeurs de cet indicateur pourraient être améliorées. / No abstract available

O objetivo deste trabalho foi avaliar o selamento apical em 7 e 30 dias de, obturações de canais radiculares com a técnica de cone único utilizando 4 cimentos endodônticos, AH Plus, MTA Fillapex, cimento Portland, e cimento experimental Sealepox, por meio do sistema de infiltração de fluidos FLODEC, e a qualidade de obturação, em microscopia confocal a laser, analisando o perímetro de penetração dos cimentos nos túbulos dentinários, e a porcentagem de fendas na interface cimento/dentina, e por meio de estereomicroscopia, a porcentagem de cimento, guta-percha e espaços vazios a 2,4 e 6mm do ápice radicular, dos cimentos. Sessenta e oito dentes unirradiculados, com canais únicos tiveram seus canais instrumentados e, então, obturados com os cimentos, previamente corados com Rodamina B. As amostras foram conservadas em meio úmido a 37º de temperatura. Após 7 dias, os dentes foram adaptados para os testes de infiltração de fluidos, fazendo-se as leituras durante 12 minutos constantes, para todos os espécimes. Trinta dias depois, as leituras foram realizadas novamente com os mesmos parâmetros anteriores. Todos os espécimes foram cortados em secções de 2mm de espessura nos níveis de 2,4 e 6mm do ápice radicular. Mediante estereomicroscopia, foram calculadas as porcentagens de cimento, guta-percha e espaços vazios, e em microscopia confocal, as porcentagens de fendas e o perímetro de penetração dos cimentos no interior dos túbulos dentinários, em todas as secções. Os resultados obtidos mostraram que, todos os cimentos permitiram a infiltração de fluidos em 7 dias, sendo que, o MTA Fillapex exibiu, significativamente, menor infiltração que os cimentos AH Plus e Portland. Em 30 dias não houve diferenças estatísticas entre os cimentos. Nas avaliações em microscopia confocal, observou-se a presença de fendas na interface dentina/cimento em todas as secções avaliadas. À estereomicroscopia observaram-se espaços vazios em todas as secções, bem como, variações nas porcentagens de cimento e guta-percha. / The aim of this study was to evaluate sealing-ability at 7 and 30 days of root canal obturations using a single cone obturation technique of 4 endodontic sealers, AH Plus, Mta Fillapex, Portland Cement and a experimental sealer Sealepox, using a fluid filtration recording device (FLODEC), to evaluate the obturation quality using confocal laser microscopy analyzing interfacial adaptation of sealer/dentin interface, perimeter of sealer penetration into dentinal tubules, and using stereomicroscopy, the percentage of sealer, guta-percha and voids at 2, 4 and 6mm from de apex. Sixtyeight single rooted teeth with one canal were instrumented and then, obturated, with the sealers, in which, Rodamine B was previously added. Samples were stored in 100% humidity and at 37º temperature. After 7 days, all the specimens were submitted to microleakage tests for a 12-minute period. Mta Fillapex showed the lowest leakage percentage when compared with AH Plus and Portland cement. 30 days after the first test, microleakage evaluations were repeated, and the results showed no statistical differences. Then, all samples were cut horizontally of 2mm of thickness at 2,4,6mm from the apex. Using estereomicrocopy, percentage of sealer, guta-percha and voids were measured, and using confocal laser microscopy percentages of interfacial adaptation and perimeter of sealer penetration into dentinal tubules were calculated. According to the results it was concluded that all sealers permitted fluid filtration at 7 and 30 days, being that, at 7 days, MTA Fillapex exhibited significantly, less fluid filtration than AH Plus and Portland Cement. At 30 days, no statistically differences were found. On confocal evaluations, it was observed the presence of gaps at the sealer/dentin interface in all evaluated sections. Stereomicroscopy assessments showed voids in all sections and as well variations on sealer and guta-percha percentages.

Das Ziel der hier durchgeführten Studie war es, die Möglichkeiten der zerstörungsfreien Untersuchungsmethode, 3-D-Röntgenmikrotomographie, bei der Bewertung von Wurzelkanalfüllungen anhand der modifizierten lateralen Kondensation, der lateralen Kondensation und bei Thermafil zu untersuchen. Es sollte gezeigt werden, dass die einzelnen Bestandteile der Wurzelkanalfüllung zu differenzieren und im Anschluss auch zu quantifizieren sind. Die ersten 21 mit dieser zerstörungsfreien Methode untersuchten Proben wurden im Anschluss nochmals mit einer klassischen Untersuchungsmethode, der Lichtmikroskopie, beurteilt. Die hier erzielten Ergebnisse wurden bei einem Methodenvergleich mit den Ergebnissen der 3-D-Röntgenmikrotomographie verglichen und auf eine mögliche Korrelation überprüft. Ziel war es zu überprüfen, ob verschiedene Methoden zur Qualitätsprüfung von Wurzelkanalfüllungen als gleichwertig angenommen werden können. Im Rahmen dieser Untersuchung wurde eine neue modifizierte laterale Kondensation eingeführt, die hinsichtlich ihrer Qualität anhand der bereits genannten Parameter mit zwei bereits eingeführten Wurzelkanalfüllmethoden verglichen werden sollte. Die 3-D-Röntgenmikrotomographie ermöglichte eine selektive Darstellung der einzelnen Komponenten in 400 bis 750 Ebenen, welche ebenfalls quantifizierbar waren. Es wurden Füllgrade zwischen 71 % und 92 % erzielt. Das Thermafil-System schien den auf lateraler Kondensation basierenden Systemen bezüglich der bestimmten Volumen vereinzelt signifikant überlegen. Bei der lichtmikroskopischen Auswertung ergaben sich diesbezüglich ähnliche Ergebnisse. Die Farbstoffpenetrationstiefen zeigten weder tendenzielle noch signifikante Unterschiede zwischen den einzelnen Fülltechniken. Beim Vergleich der Methoden zur Bewertung der Obturation bezüglich der erhobenen Volumen konnte keine Korrelation nachgewiesen werden. Auch beim Vergleich der Farbstoffpenetrationstiefen und der bestimmten Volumen bei der 3-D-Röntgenmikrotomographie konnten keine Korrelationen ermittelt werden. Unter Berücksichtigung der Grenzen dieser Untersuchung lässt sich festhalten, dass die Methode der 3-D-Röntgenmikrotomographie zur Bewertung und zum Vergleich von Wurzelkanalfüllungen geeignet ist. Die Ergebnisse korrelieren jedoch nicht mit denen der Lichtmikroskopie.

O objetivo deste trabalho foi avaliar o selamento apical em 7 e 30 dias de, obturações de canais radiculares com a técnica de cone único utilizando 4 cimentos endodônticos, AH Plus, MTA Fillapex, cimento Portland, e cimento experimental Sealepox, por meio do sistema de infiltração de fluidos FLODEC, e a qualidade de obturação, em microscopia confocal a laser, analisando o perímetro de penetração dos cimentos nos túbulos dentinários, e a porcentagem de fendas na interface cimento/dentina, e por meio de estereomicroscopia, a porcentagem de cimento, guta-percha e espaços vazios a 2,4 e 6mm do ápice radicular, dos cimentos. Sessenta e oito dentes unirradiculados, com canais únicos tiveram seus canais instrumentados e, então, obturados com os cimentos, previamente corados com Rodamina B. As amostras foram conservadas em meio úmido a 37º de temperatura. Após 7 dias, os dentes foram adaptados para os testes de infiltração de fluidos, fazendo-se as leituras durante 12 minutos constantes, para todos os espécimes. Trinta dias depois, as leituras foram realizadas novamente com os mesmos parâmetros anteriores. Todos os espécimes foram cortados em secções de 2mm de espessura nos níveis de 2,4 e 6mm do ápice radicular. Mediante estereomicroscopia, foram calculadas as porcentagens de cimento, guta-percha e espaços vazios, e em microscopia confocal, as porcentagens de fendas e o perímetro de penetração dos cimentos no interior dos túbulos dentinários, em todas as secções. Os resultados obtidos mostraram que, todos os cimentos permitiram a infiltração de fluidos em 7 dias, sendo que, o MTA Fillapex exibiu, significativamente, menor infiltração que os cimentos AH Plus e Portland. Em 30 dias não houve diferenças estatísticas entre os cimentos. Nas avaliações em microscopia confocal, observou-se a presença de fendas na interface dentina/cimento em todas as secções avaliadas. À estereomicroscopia observaram-se espaços vazios em todas as secções, bem como, variações nas porcentagens de cimento e guta-percha. / The aim of this study was to evaluate sealing-ability at 7 and 30 days of root canal obturations using a single cone obturation technique of 4 endodontic sealers, AH Plus, Mta Fillapex, Portland Cement and a experimental sealer Sealepox, using a fluid filtration recording device (FLODEC), to evaluate the obturation quality using confocal laser microscopy analyzing interfacial adaptation of sealer/dentin interface, perimeter of sealer penetration into dentinal tubules, and using stereomicroscopy, the percentage of sealer, guta-percha and voids at 2, 4 and 6mm from de apex. Sixtyeight single rooted teeth with one canal were instrumented and then, obturated, with the sealers, in which, Rodamine B was previously added. Samples were stored in 100% humidity and at 37º temperature. After 7 days, all the specimens were submitted to microleakage tests for a 12-minute period. Mta Fillapex showed the lowest leakage percentage when compared with AH Plus and Portland cement. 30 days after the first test, microleakage evaluations were repeated, and the results showed no statistical differences. Then, all samples were cut horizontally of 2mm of thickness at 2,4,6mm from the apex. Using estereomicrocopy, percentage of sealer, guta-percha and voids were measured, and using confocal laser microscopy percentages of interfacial adaptation and perimeter of sealer penetration into dentinal tubules were calculated. According to the results it was concluded that all sealers permitted fluid filtration at 7 and 30 days, being that, at 7 days, MTA Fillapex exhibited significantly, less fluid filtration than AH Plus and Portland Cement. At 30 days, no statistically differences were found. On confocal evaluations, it was observed the presence of gaps at the sealer/dentin interface in all evaluated sections. Stereomicroscopy assessments showed voids in all sections and as well variations on sealer and guta-percha percentages.