Le travail de thèse s’inscrit dans la problématique très actuelle des mortalités massives de naissain et de juvéniles d’huîtres creuses, C. gigas, et des questionnements autour de l’implication des pesticides dans ce phénomène. La première partie de la thèse a été consacrée à l’étude des effets de pesticides sur les capacités hémocytaires de l’huître creuse (in vitro et in vivo). L’effet immuno-modulateur des xénobiotiques sélectionnés (seul ou en mélange) a été exploré principalement au travers de la cytométrie en flux. La deuxième partie de la thèse a concerné l’étude des effets d’un mélange de pesticides sélectionnés sur le virus OsHV-1 et l’infection qu’il induit chez l’huître creuse. Les effets des pesticides sur le virus lui-même ont été évalués. Les expériences réalisées n'ont pas permis de mettre en évidence, dans les conditions testées, de dégradation des particules virales en présence des polluants. D’autre part, leurs effets sur les huîtres elles-mêmes ont été explorés après traitement des animaux avec un mélange de pesticides lors d’essais de pathologie expérimentale. Il a ainsi pu être montré que des huîtres préalablement contaminées par un mélange de pesticides à des concentrations retrouvées dans l’environnement semblaient être plus sensibles à l’infection virale. La troisième partie de la thèse a concerné l’étude de l’autophagie chez l’huître creuse, C. gigas. La publication du génome complet de cette espèce en 2012 a ouvert de nouvelles possibilités pour étudier l'immunité innée. L’étude de l’autophagie réalisée pour la première fois chez l’huître creuse a consisté lors d’une première étape en la recherche in silico de gènes impliqués dans cette voie et des protéines correspondantes par western blotting. Puis, le rôle de ce processus important dans l’immunité innée a été exploré au travers d’essais de reproduction d’infections en présence ou non de modulateurs de l’autophagie. Les résultats obtenus semblent montrer que l’autophagie soit un processus important pouvant être impliqué dans les capacités de défense de l’huître creuse vis-à-vis d’infections virales et bactériennes. / The thesis work is part of the very current issue on mass mortality outbreaks affecting Pacific oyster, C. gigas, spat and juveniles and questions about the involvement of pesticides in this phenomenon. The first part of this thesis was devoted to study the effects of pesticides on hemocyte parameters in the Pacific oyster (in vitro and in vivo). The immunomodulator effect of selected pesticides (alone or in mixture) has been explored principally through flow cytometry. The second part concerned the study of the effects of a pesticides mixture on OsHV-1 itself. No direct effects have been reported on the viral particles in presence of the pollutants. Moreover, pesticide effects on Pacific oysters were also explored through experimental pathology assays after treatment of animals with a polluant mixture. Results showed that pesticide treated oysters appeared more susceptible to the viral infection in experimental conditions. The third part concerned the study of autophagy in the Pacific oyster, C. gigas. The publication of the complete genome in 2012 has opened up new possibilities for the study of innate immunity in this species. The study of autophagy for the first time in oysters consisted in a first step in the in silico search for genes involved in this pathway and the corresponding proteins by Western blotting. Then, the role of this important process in innate immunity has been explored through reproduction infections tests with or without modulators of autophagy. Results showed that autophagy appeared as involved in defence mechanisms against viral and bacterial infection in Pacific oysters.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-14T14:32:41Z No. of bitstreams: 1 Izabel Cristina da Silva Almeida.pdf: 3784157 bytes, checksum: 9dd220764f324bfa5ddd8bbf00714adf (MD5) / Made available in DSpace on 2017-02-14T14:32:41Z (GMT). No. of bitstreams: 1 Izabel Cristina da Silva Almeida.pdf: 3784157 bytes, checksum: 9dd220764f324bfa5ddd8bbf00714adf (MD5) Previous issue date: 2006-10-09 / This work aimed to delimit collective aquaculture areas to the “native oyster” Crassostrea rhizophorae farming, as part of the Mariculture Development Local Plan in the Pernambuco’s north coast. Indicators were identified, selected and hierarquized to enable the elaboration of a conceptual model subsidizing the selection process of potential oyster farming areas. Depth measurements were realized in transversal sections of the estuarine channels using ecobathimeter linked to a GPS (Global Positioning System) and a Palmtop. In the of aquaculture area demarcation was used eight environmental indicators, seven socioeconomic and five of production/logistics to subsidize the selection process of the oyster farming potential areas. Three preferential areas were delimited, in which six collective aquaculture areas were demarcated, being three in the municipality of Goiana (estuary of the river Itapessoca), two in Igarassu (estuary of the river Timbó) and one in Itapissuma (Santa Cruz channel), where 263 individual aquaculture areas could be inserted, totalizing 77,2 ha of collective aquaculture areas. / O presente trabalho visa a delimitação parques aqüícolas para o cultivo da “ostra nativa” Crassostrea rhizophorae os quais integram o Plano Local de Desenvolvimento da Maricultura no litoral norte de Pernambuco. Foram identificados, selecionados e hierarquizados descritores que possibilitaram a elaboração de um modelo conceitual subsidiando o processo de seleção de áreas potenciais para fins de ostreicultura. Realizou-se a batimetria nas secções transversais dos canais estuarinos selecionados com o auxílio de um ecobatímetro acoplado a GPS (Global Positioning System) e Palmtop. Posteriormente, com um GPS geodésico foi realizada a demarcação dos parques aqüícolas. Identificaram-se oito descritores ambientais, sete sócio-econômicos e cinco de produção/logística capazes de subsidiar no processo de seleção das áreas potenciais ao cultivo de ostra. Foram delimitadas três faixas de preferência, nas quais demarcaram-se seis parques aqüícolas, sendo três pertencentes ao município de Goiana (estuário do rio Itapessoca), dois de Igarassu (estuário do rio Timbó) e um de Itapissuma (canal de Santa Cruz.), onde poderão ser inseridas 263 áreas aqüícolas, totalizando 77,2 ha de parques aqüícolas.

<p>The pearl oyster <em>Pinctada martensii </em>or <em>Pinctada fucata </em>is the oyster for produce the South China Sea Pearl, and the production of pearl oyster <em>Pinctada martensii</em> plays a key role for the economic and social welfare of the coastal areas. To guarantee both rich and sustainability of providing pearl oyster productions, addressing the suitable areas for aquaculture is a very important consideration in any aquaculture activities. Relatively rarely, in the case of site selection research, the researchers use GIS analysis to identify suitable sites in fishery industry in China. Therefore, I decided to help the local government to search suitable sites form the view of GIS context. This study was conducted to find the optimal sites for suspended culture of pearl oyster <em>Pinctada martensii </em>using GIS-based multi-criteria analysis. The original idea came from the research of Radiarta and his colleagues in 2008 in Japan. Most of the parameters in the GIS model were extracted from remote sensing data (Moderate Resolution Imaging Spectroradiometer and Landsat 7). Eleven thematic layers were arranged into three sub-models, namely: biophysical model, social-economic model and constraint model. The biophysical model includes sea surface temperature, chlorophyll-α concentration, suspended sediment concentration and bathymetry. The criteria in the social-economic model are distance to cities and towns and distance to piers. The constraint model was used to exclude the places from the research area where the natural conditions cannot be fulfilled for the development of pearl oyster aquaculture; it contains river mouth, tourism area, harbor, salt fields / shrimp ponds, and non-related water area. Finally those GIS sub-models were used to address the optimal sites for pearl oyster <em>Pinctada martensii</em> culture by using weighted linear combination evaluation. In the final result, suitability levels were arranged from 1 (least suitable) to 8 (most suitable), and about 2.4% of the total potential area had the higher levels (level 6 and 7). These areas were considered to be the places that have the most suitable conditions for pearl oyster <em>Pinctada martensii </em>for costal water of Yingpan.</p>

The pearl oyster Pinctada martensii or Pinctada fucata is the oyster for produce the South China Sea Pearl, and the production of pearl oyster Pinctada martensii plays a key role for the economic and social welfare of the coastal areas. To guarantee both rich and sustainability of providing pearl oyster productions, addressing the suitable areas for aquaculture is a very important consideration in any aquaculture activities. Relatively rarely, in the case of site selection research, the researchers use GIS analysis to identify suitable sites in fishery industry in China. Therefore, I decided to help the local government to search suitable sites form the view of GIS context. This study was conducted to find the optimal sites for suspended culture of pearl oyster Pinctada martensii using GIS-based multi-criteria analysis. The original idea came from the research of Radiarta and his colleagues in 2008 in Japan. Most of the parameters in the GIS model were extracted from remote sensing data (Moderate Resolution Imaging Spectroradiometer and Landsat 7). Eleven thematic layers were arranged into three sub-models, namely: biophysical model, social-economic model and constraint model. The biophysical model includes sea surface temperature, chlorophyll-α concentration, suspended sediment concentration and bathymetry. The criteria in the social-economic model are distance to cities and towns and distance to piers. The constraint model was used to exclude the places from the research area where the natural conditions cannot be fulfilled for the development of pearl oyster aquaculture; it contains river mouth, tourism area, harbor, salt fields / shrimp ponds, and non-related water area. Finally those GIS sub-models were used to address the optimal sites for pearl oyster Pinctada martensii culture by using weighted linear combination evaluation. In the final result, suitability levels were arranged from 1 (least suitable) to 8 (most suitable), and about 2.4% of the total potential area had the higher levels (level 6 and 7). These areas were considered to be the places that have the most suitable conditions for pearl oyster Pinctada martensii for costal water of Yingpan.

With the majority of Earth’s population living in coastal areas, estuarine ecosystems have been particularly affected by anthropogenic disturbances. My dissertation research focused on three interrelated types of human disturbance that affect estuaries: Anthropogenic alteration of freshwater inflow, the introduction of invasive species, and habitat alteration. Using the LoxahatcheeRiver(Jupiter, FL) as a model system, my goal was to understand how these disturbances affect estuarine organisms, particularly fishes. One of the most ecologically harmful disturbances affecting estuaries is anthropogenic alteration of freshwater inflow (and resulting changes in salinity patterns). To identify effects of freshwater inflow on the behavior of an ecologically and economically important fish (common snook Centropomus undecimalis), I conducted a 19-month acoustic telemetry study. Common snook were more abundant and made more frequent upstream migrations during the wet season, but freshwater inflow did not appear to be the proximate cause for these behaviors. Increased estuarine salinity resulting from anthropogenic flow alteration may have facilitated the second type of disturbance that I address in this dissertation; the invasion of non-native Indo-Pacific lionfish into estuarine habitats. During the course of my dissertation research, I documented the first ever estuarine invasion by non-native lionfish. Using mark-recapture, I identified high site fidelity in lionfish, a trait that may aid future control efforts. The extremely low minimum salinity tolerance that I identified in lionfish appears to have allowed the species to colonize far upriver in estuaries with anthropogenically modified salinity patterns. Anthropogenic salinity alteration has also led to a severe degradation of oyster reef habitats in theLoxahatcheeRiver. As a foundation species, oysters provide food, shelter, and nursery habitat for a wide variety of estuarine organisms, including many ecologically and economically important fishes. Increasingly, degraded oyster reef habitats have been the focus of restoration efforts. I identified a relatively rapid (< 2 years) convergence between restored and natural oyster reef communities, and documented the importance of vertical relief in restoration success. My dissertation research is critical for the management and conservation of coastal rivers inFlorida, while more broadly informing restoration and management decisions in many other estuarine and coastal ecosystems.

One of the countries where the basic academic education is problematic to achieve is Tanzania. Despite the multiple attempts to increase enrolments and decrease dropouts through new partnerships and organizations, high level of drop out still occur in the country. Two research questions were composed in aim to understand why this drop out phenomenon still occur. First question: “Which individual/ collective factors contribute to that girls and boys in the age group between 7 and 13 drop out of  primary schools?” and the second question: “What differences are there in the drop out causes between two more “privileged” and one more “marginalized” school?”.  The answers to these questions were found through the field work in Babati Town Council, Tanzania. There primary data was gathered in form of semi-structured interviews with three headteachers from three different schools. Two privileged schools; Maisaka Primary School and Primary School Oyster bay, and one marginalized school; Kiongozi Primary School. Also interviews with nine parents/other guardians of dropouts were conducted. Gathered data was summarized in short case stories, narratives which later on were analyzed with in a theoretical framework. This theoretical framework consisted of six different theories. These theories are; Academic Mediation theory, General Deviance theory, Deviant Affiliation theory, Poor Family Socialization theory, Structural Strains theory and lastly a theory of Institutional Departure. The conclusion of the study is that, even though the schools are located in the same area a major difference in dropout rates and its causes are existing. At the marginalized school there is a high level of drop out and at more privileged schools no dropouts occur. Factors which play a major role for this existing difference are; childrens and parents commitment to school, family structure and background, family's socio-economic status, relationships in the family and of course quality of the school.

Made available in DSpace on 2014-10-09T12:33:02Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:06:05Z (GMT). No. of bitstreams: 0 / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Made available in DSpace on 2014-06-11T19:29:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-02Bitstream added on 2014-06-13T20:59:58Z : No. of bitstreams: 1 figueiro_gg_me_ilha.pdf: 1622216 bytes, checksum: 6223c00cd61193ae623babff3571f19a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O presente trabalho teve por objetivo evidenciar os principais componentes químicos que influenciam o cultivo do cogumelo Pleurotus florida, bem como avaliar a composição química dos corpos de frutificação cultivados em diferentes resíduos agrícolas. Para tanto, foram utilizados seis resíduos agrícolas: palha de arroz (PA), palha de feijão (PF), palha de trigo (PT), palha de sorgo (PS), folha de bananeira (FB) e sabugo de milho (SM). Estes resíduos, coletados na Fazenda de Ensino, Pesquisa e Extensão da UNESP, Campus de Ilha Solteira, foram analisados quanto aos teores de macro (N, P, K, Ca, Mg) e micronutrientes (Fe, Mn e Zn), lignina, celulose, hemicelulose, cinzas e relação C/N. Foram avaliados: o tempo necessário para a colonização do substrato (corrida micelial), o início da formação de primórdios, o tempo total de cultivo, o número de cogumelos, a produção e a eficiência biológica utilizando a fórmula: EB = (peso fresco dos cogumelos/peso seco do substrato inicial) x 100. Além disso, os cogumelos foram avaliados quanto aos teores de macro (N, P, K, Ca e Mg) e micronutrientes (Fe, Mn e Zn) e o teor de proteínas. O substrato PF apresentou resultados semelhantes para produção (189,8 g/kg-1), EB (89,2%) e número de cogumelos (12) à PA, substrato utilizado tradicionalmente no cultivo de Pleurotus em escala comercial. Não foi possível atribuir apenas a um fator químico as altas produções e EB observadas em PA e PF e muito menos para a baixa produção em PS (77,8 g/kg-1) e SM (53,2 g/kg-1). No geral, substratos com conteúdo de N ao redor de 1,0%, relação C/N em torno de 45%, baixo teor lignina, alto conteúdo de cinzas e maiores teores de P, K e Ca foram os melhores para o cultivo de P. florida. Entre os macronutrientes analisados, P. florida apresentou maiores teores de K, seguido por P. O Ca e o Mg estiveram presentes em pequenas quantidades nos corpos... / This study aimed to show the main chemical components that influence the cultivation of the mushroom Pleurotus florida, and evaluate the chemical composition of fruiting bodies grown on different agricultural residues. For that, six agricultural residues were used: rice straw (RS), bean straw (BS), wheat straw (WS), sorghum straw (SS), banana leaf (BL) and cob of maize (CM). These wastes were collected at Teaching, Research and Extention Farm of UNESP, Campus of Ilha Solteira, analyzed for the levels of macro (N, P, K, Ca, and Mg) and micronutrients (Fe, Mn and Zn), lignin, cellulose, hemicellulose, ash and the C/N ratio. Was evaluated: spawn run time, earliness, the total time of cultivation and mushroom number, production and biological efficiency using the formula: EB = (fresh weight of mushrooms/dry weight of initial substrate) x 100. Moreover, the mushrooms were evaluated for levels of macro (N, P, K, Ca and Mg) and micronutrients (Fe, Mn and Zn) and protein content. The substrate BS showed similar results for yield (189,8 g/kg-1), EB (89,2%) and number of mushrooms (12) as RS substrate traditionally used for the cultivation of Pleurotus in commercial scale. Unable to allocate only the chemical factors and the high yields and EB observed in BS and RS, much less for the yield low in SS (77,8 g/kg-1) and CM (53,2 g/kg-1). In general, substrates with N content of around 1.0%, C/N ratio around 45%, low lignin content, high ash content, increased by higher levels of P, K, Ca were the best for the cultivation of P. florida. Among the macronutrients analyzed, P. florida with higher contents of K, followed by P. The Ca and Mg were present in small amounts in fruiting bodies. Among the micronutrients, Zn was present in high amount, followed by Fe and Mn. P. florida showed high ability to accumulate Zn. The mushrooms obtained in this work could not be considered a source of minerals... (Complete abstract click electronic access below)

Made available in DSpace on 2014-10-09T12:33:02Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:06:05Z (GMT). No. of bitstreams: 0 / O ambiente costeiro tem sido fortemente alterado em função de múltiplos impactos ambientais decorrentes das atividades antrópicas, como por exemplo, o escoamento de esgoto proveniente de áreas urbanas, a liberação de inúmeros produtos químicos das indústrias, a agricultura e o fluxo de embarcações, que podem acarretar vazamentos acidentais de petróleo e derivados, combustíveis e outros produtos transportados por via marítima. Neste contexto, um dos meios para avaliação da contaminação ambiental causada por essas substâncias potencialmente tóxicas em água do mar é a monitoração por meio de diferentes espécies de bivalves, que tem sido utilizada por diversos pesquisadores, do Brasil e do exterior. No que se refere aos moluscos bivalves, particularmente os mexilhões, o seu uso no biomonitoramento da contaminação marinha deve-se principalmente à sua ampla distribuição geográfica, hábito séssil e habilidade de concentrar metais tóxicos em até 102 - 105 vezes em relação às concentrações detectadas na água. No presente trabalho, foi empregado o biomonitoramento passivo com o uso do molusco bivalve Perna perna com relação aos elementos Br, Cl, K, Mg, Mn e V. Foram escolhidos estes elementos uma vez que eles podem ser determinados pelo método de INAA (Análise por Ativação com Nêutrons Instrumental), por meio da irradiação curta o que proporciona análises mais rápidas e também por serem importantes do ponto de vista nutricional ou ambiental. O objetivo do presente trabalho foi avaliar os teores de Br, Cl, K, Mg, Mn e V em amostras de mexilhões Perna perna (Linnaeus, 1758: Mollusca, Bivalvia) coletados em regiões costeiras de São Paulo sujeitas à contaminação antrópica (Ponta de Itaipu e Ilha das Palmas, em Santos), comparando os valores obtidos nos sítios possivelmente impactados com os valores do sítio controle situado na Praia da Cocanha, em Caraguatatuba. Os pontos de coleta no litoral de São Paulo estão localizados nas regiões geográficas 23º 37 S 45º 24 W (Caraguatatuba) e 23º 57\' S - 46º 20\' W (Santos). A coleta dos organismos foi realizada em todas as estações do ano, com início na primavera de 2008 e término no inverno de 2009. As amostras coletadas foram limpas, trituradas, homogeneizadas e secas por meio da liofilização para posterior INAA. O procedimento de INAA consistiu em irradiar alíquotas das amostras obtidas na forma de pó em invólucros de polietileno no reator nuclear IEA R1 do IPEN/CNEN - SP juntamente com padrões sintéticos de elementos. O tempo de irradiação no reator foi de 8 a 10 s e sob o fluxo de nêutrons térmicos de 6,6 x 1012 n cm-2 s-1. As concentrações dos elementos foram calculadas pelo método comparativo. O procedimento de INAA foi validado com relação à exatidão e precisão, por meio das análises dos materiais de referência certificados NIST SRM 1566b Oyster Tissue e NIST SRM 2876 Mussel Tissue. Os resultados destes materiais de referência certificados indicaram uma boa qualidade dos resultados com relação à precisão e exatidão. As faixas das concentrações (em base seca) dos elementos obtidos nos mexilhões coletados em Santos e na Praia da Cocanha para as quatro estações do ano foram de: 173,80 a 358,99 mg kg-1 para Br; 45658 ± 1811 a 109166 ± 824 mg kg-1 para Cl; 7043 ± 856 a 12506 ± 675 mg kg-1 para K; 2774 ± 211 a 5691 ± 717 mg kg-1 para Mg; 7,01 ± 0,30 a 29,74 ± 3,32 mg kg-1 para Mn e 0,77 ± 0,02 a 3,43 ± 0,28 mg kg-1 para V. Foi estudada a variação sazonal e espacial das concentrações desses elementos e também foram comparados esses resultados com valores da literatura. A partir dos resultados obtidos pode - se concluir que os mexilhões da espécie Perna perna, podem ser utilizados como biomonitores da contaminação marinha. / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

L'huître creuse C. gigas a connu des épisodes récurrents de mortalités estivales. En 2009, l'ostréiculture a connu la plus grave crise écologique et économique jamais observée depuis l'introduction de cette espèce sur les côtes françaises. Les études réalisées précédemment dans le but de comprendre les causes de ces mortalités, attribuent une origine multifactorielle faisant intervenir, de manière concomitante, des paramètres environnementaux, des conditions physiologiques particulières de l'huître, en association à la présence de microorganismes pathogènes tels que les vibrions appartenant aux espèces V. aestuarianus, V. splendidus et le virus OsHV-1. Ainsi, l'ensemble des travaux menés au cours de cette thèse représentent une contribution à l'étude d'une réponse immunitaire efficace de l'huître creuse Crassostrea gigas aux infections vibrions pathogènes.Le travail de thèse qui vous a été présenté a été réalisé dans le but de progresser encore sur la compréhension de la réponse immunitaire de l'huître creuse. Ce travail nous a permis d'établir une cartographie des modifications transcriptionnelles par la technique DGE (Digital Gene Expression) et notamment par l'identification d'effecteurs ou mécanismes entrant en jeu dans une réponse efficace vis-à-vis des agents infectieux et conduisant à son élimination. Le modèle (Crassostrea gigas – V. aestuarianus/V. splendidus) s'est avéré adapté à cette étude. Les mécanismes identifiés au cours de cette thèse, devront être étudiés plus précisément, puisque la plupart des gènes surreprésentés chez les huîtres survivantes ont des fonctions putatives. Nous avons suggéré l'implication de ces gènes dans le processus de survie des huîtres au cours d'une réponse efficace aux infections bactériennes. Il est apparu nécessaire de développer des recherches autour des défenses de l'huître creuse C. gigas avec pour objectif le développement de stratégies destinées à limiter l'impact des maladies. A long terme, ces stratégies seront applicables soit en prophylaxie afin de détecter des déficiences et prévenir le développement de maladies, soit en sélection génétique pour le criblage de géniteurs présentant des capacités de défenses optimales. / Aquatic organisms and particularly marine invertebrates, such as the oyster Crassostrea gigas, harbour an abundant and diverse microflora on their surface (epibiosis) or inside their tissues (endobiosis) where Vibrio splendidus is found as a dominant culturable Vibrio. With Evolution, oysters have developed effective systems for maintaining their homeostasis and discriminating the bacteria beneficial for their physiological fitness from the potentially harmful and pathogenic ones. However, for decades, the cultivated Pacific oyster C. gigas is suffering large scale summer mortality phenomenon that is reported in all areas of the world where this species is cultivated. Considerable effort has been invested in advanced genomic technologies to understand and characterize the major traits that govern the tolerance of oysters to stressful culture conditions and to pathogenic bacteria. In particular, immune-related genes have been characterized from C. giga. Briefly, a variety of antimicrobials have been fully characterized. Whereas most of these immune genes were shown to be modulated during infections, the molecular mechanisms by which the oyster can survive virulent Vibrio infections remained totally unknown. Here, our objective was to develop a better understanding of the genetic-level responses of oysters to pathogenic versus non pathogenic bacteria and to identify genes that are involved in physiological and immune responsiveness to circumvent the infections. In this attempt, we have performed a comprehensive analysis of the transcriptome of oyster immunity (hemocytes), using Digital Gene Expression (DGE). The study aimed to compare the expression profiles of the two libraries and, beyond gene identification and functional annotation, to explore the putative functions involved in the capability of oysters to circumvent and to survive infections. This is the first report on genome-wide transcriptional analysis of oyster survival-responsiveness.