1 |
Esporos de Bacillus subtilis como adjuvante vacinal. / Bacillus subtilis spores as a vaccine adjuvante.Souza, Renata Damasio de 09 October 2014 (has links)
Esporos de Bacillus subtilis apresentam propriedades adjuvantes, sendo capazes de aumentar a resposta humoral após a sua coadministração com antígenos misturados ou adsorvidos à sua superfície. Mas, para isso, é necessária a produção de esporos altamente purificados e com rendimentos elevados. Neste trabalho, realizamos com sucesso uma análise quantitativa das condições de esporulação e dos métodos de purificação, o que melhorou a reprodutibilidade do processo e a obtenção de amostras com elevado grau de pureza e rendimento. Avaliamos também as propriedades imunomodulatórias destes esporos, utilizando como antígeno modelo a proteína recombinante Gag-p24 do HIV-1. A coadministração, mas não a adsorção à superfície do esporo, aumentou a imunogenicidade do antígeno sem induzir efeitos deletérios após a administração parenteral em camundongos BALB/c e C57BL/6. Além de promoveram a ativação das APCs, os esporos interagem com receptores relacionados à imunidade inata, devido à ausência do efeito adjuvante em camundongos nocautes para TLR2. Esses resultados abrem perspectivas interessantes para a utilização de esporos como adjuvantes vacinais. / Bacillus subtilis spores have been shown to behave as vaccine adjuvants, promoting the increase of antibody responses after co-administration with antigens either admixed or adsorbed on the spore surface. Nonetheless, such specialized application requires highly purified spore preparations at high yields. In this work, we successfully performed a systematic quantitative analysis of sporulation conditions and spore purification methods, which improved the reproducibility of the process and the obtainment of samples with high purity and yield. Afterwards, we further evaluated the immune modulatory properties of these spores using a recombinant HIV-1 Gag-p24 protein as a model antigen. The co-administration, but not adsorption to the spore surface, enhanced the immunogenicity of that target antigen, without inducing deleterious effects, after subcutaneous administration to BALB/c and C57BL/6 mice. Besides promoting activation of antigen presenting cells, spores interact with receptors related to innate immunity, due to the absence of the adjuvant effect on TLR2 knockout mice. These results open interesting perspectives for the use of B. subtilis spores as vaccine adjuvants.
|
2 |
Esporos de Bacillus subtilis como adjuvante vacinal. / Bacillus subtilis spores as a vaccine adjuvante.Renata Damasio de Souza 09 October 2014 (has links)
Esporos de Bacillus subtilis apresentam propriedades adjuvantes, sendo capazes de aumentar a resposta humoral após a sua coadministração com antígenos misturados ou adsorvidos à sua superfície. Mas, para isso, é necessária a produção de esporos altamente purificados e com rendimentos elevados. Neste trabalho, realizamos com sucesso uma análise quantitativa das condições de esporulação e dos métodos de purificação, o que melhorou a reprodutibilidade do processo e a obtenção de amostras com elevado grau de pureza e rendimento. Avaliamos também as propriedades imunomodulatórias destes esporos, utilizando como antígeno modelo a proteína recombinante Gag-p24 do HIV-1. A coadministração, mas não a adsorção à superfície do esporo, aumentou a imunogenicidade do antígeno sem induzir efeitos deletérios após a administração parenteral em camundongos BALB/c e C57BL/6. Além de promoveram a ativação das APCs, os esporos interagem com receptores relacionados à imunidade inata, devido à ausência do efeito adjuvante em camundongos nocautes para TLR2. Esses resultados abrem perspectivas interessantes para a utilização de esporos como adjuvantes vacinais. / Bacillus subtilis spores have been shown to behave as vaccine adjuvants, promoting the increase of antibody responses after co-administration with antigens either admixed or adsorbed on the spore surface. Nonetheless, such specialized application requires highly purified spore preparations at high yields. In this work, we successfully performed a systematic quantitative analysis of sporulation conditions and spore purification methods, which improved the reproducibility of the process and the obtainment of samples with high purity and yield. Afterwards, we further evaluated the immune modulatory properties of these spores using a recombinant HIV-1 Gag-p24 protein as a model antigen. The co-administration, but not adsorption to the spore surface, enhanced the immunogenicity of that target antigen, without inducing deleterious effects, after subcutaneous administration to BALB/c and C57BL/6 mice. Besides promoting activation of antigen presenting cells, spores interact with receptors related to innate immunity, due to the absence of the adjuvant effect on TLR2 knockout mice. These results open interesting perspectives for the use of B. subtilis spores as vaccine adjuvants.
|
3 |
Excision margins in human immunodeficiency virus seropositive women undergoing large loop excision of the transformation zone for cervical dysplasiaNoel, Carolyn Joyce January 2015 (has links)
Department of Obstetrics and Gynaecology
University of the Witwatersrand
Johannesburg
February 2015
A research report submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in partial fulfilment of the requirements for the degree of Masters in Medicine, in the branch of Obstetrics and Gynaecology. / HIV accelerates the development of cervical cancer by up to15 years. South Africa is currently in the midst of an HIV epidemic. With limited facilities for colposcopy it is vital to identify risk factors within the HIV positive population resulting in positive margins after Large Loop Excision of the Transformation Zone (LLETZ) and persistence of cytological abnormalities on follow-up Pap smears.
Objective: The primary objective was to determine the patient risk factors, pre and during colposcopy and LLETZ biopsy, which resulted in the histological involvement of margins of the LLETZ biopsy and persistent cervical dysplasia on follow-up Pap smears. Secondary objectives included determining follow up rate of patients at the clinic as well as the correlation between the original Pap smear cytology grade and the histological grade found on histology of the LLETZ biopsy.
Methods: A retrospective review of the files of HIV seropositive patients was done at the colposcopy clinic at Charlotte Maxeke Johannesburg Academic Hospital after the roll out of antiretroviral treatment for the period 1 April 2004 to 31 October 2012. Patients with abnormal pap smears during this time were referred to the colposcopy clinic where a colposcopy and LLETZ biopsies were done. Demographic and clinical data in regards to age, gravidity, contraception, CD4 count, antiretroviral usage, and referral time was collected. Data from the clinical description of the colposcopy and histology of the LLETZ biopsy was also collected. Patients followed up again after 6 months when a repeat pap smear was done. The results of these Pap smears were also collected. Data was then analysed and variate and multivariate logistical regression was used to find statistically significant correlations.
Results: A total of 480 files were found to have complete clinical records. One hundred and sixty eight (42.71%) patients had both endo and ectocervical margins clear. Predictive factors for the involvement of endocervical margins was the doctor performing the procedure (p-value <0.01) cytology of the original Pap smear (p value <0.01) and the grade of histological abnormality found at time of LLETZ (p-value <0.01). The statistically significant predictive factors for ectocervical margin involvement was the visualization of the transformation zone at colposcopy (p-value <0.01), the size of lesion found at colposcopy (p-value <0.01), the use of combined oral contraceptive pill (OCP) (p-value 0.02) and the histological grade of abnormality found on the LLETZ biopsy. Age, parity, CD4 count, use of antiretroviral drugs, length of time from Pap smear to colposcopy and use of contraception other than OCP were not found to be statistically significant in our sample population for the involvement of either endo or ectocervical margins.
Statistically significant risk factors for the recurrence of intraepithelial lesions on follow up Pap smear was having both endo and ectocervical margin involvement on histology (p-value 0.01) The Ectocervical margin alone was found to have a p-value of <0.01. Abnormal cytology on follow up Pap smear was found in 58.69% of patients.
The follow up rate at the clinic was 46.04%.
Correlation of cytological grade and histological grade of cervical intraepithelial neoplasm in our sample population was found to be adequate (p-value <0.01).
Conclusion: Incomplete incision of the intraepithelial lesion was found to be a significant risk factor for the recurrence of cytological abnormality in patients undergoing LLETZ biopsy. Identifying patients at increased risk for recurrence is important to ensure close follow up in this patient population.
|
4 |
Construção e caracterização imunológica de formulação vacinal com propriedade profiláticas voltada para o controle do vírus da imunodeficiência humana (HIV) e do vírus herpes (HSV). / Construction and immunological characterization of a vaccine formulation with prophylactic properties aiming to control the human immunodeficiency virus (HIV) and herpes virus (HSV).Cariri, Francisco André Marques de Oliveira 13 August 2014 (has links)
O presente projeto propõe a avaliação de respostas imunológicas induzidas por uma vacina de DNA bivalente para o controle de infecções por HIV e HSV. A vacina genética codifica a proteína gD do vírus HSV-1 geneticamente fusionada à proteína Gag (p24) do HIV, rica em epítopos reconhecidos por células T CD8+. A vacina de DNA pRE4p24 codifica a proteína p24 inserida em região próxima à porção C-terminal da proteína gD-1, permitindo que a proteína recombinante seja expressa na superfície da célula transfectada. A localização da proteína recombinante foi confirmada na superfície das células HEK-293T transfectadas por ensaios de imunofluorescência. Camundongos imunizados com a vacina foram capazes de gerar respostas de anticorpos específicos após três doses administradas pelas vias intramuscular (i.m.), intradérmica com seringa (i.d.) ou intradérmico por biobalística (gg). Foram analisadas as respostas imunológicas mediadas por linfócitos T CD8+ p24-específicas e, em função dos resultados obtidos, sendo a via i.m. escolhida como a mais promissora para os ensaios subsequentes. Na tentativa de aumentar a imunogenicidade da vacina, particularmente, para respostas celulares, foi avaliado o efeito da co-administração da formulação vacinal com outros plasmídeos que expressam citocinas (pIL-2, pIL-12 ou pGM-CSF), o teste de um vetor vacinal baseado no plasmídeo multicópia pVAX (pgDp24) e o emprego de um gene sintético para promover o aumento da expressão da proteína gD em células de mamíferos (pgDhp24). Por fim, desenvolvemos um modelo murino para a avaliação funcional de respostas citotóxicas antígeno-específicas a partir de uma linhagem tumoral capaz de expressar a proteína p24 do HIV. Camundongos Balb/c imunizados com o pgDp24 apresentaram um retardo no crescimento tumoral em relação aos animais não imunizados além de proteção parcial a desafios letais com HSV-1. / The present thesis aims to evaluate the immunological responses induced by a bivalent DNA vaccine to the control HIV and HSV infectious. This genetic vaccine codes the gD protein from the HSV-1 virus envelope genetically fused with HIV Gag (p24) protein, which has various epitopes recognized by human and murine T CD8+ cells. The DNA vaccine, named pRE4p24, codes for p24 protein, inserted close to the C-terminal region of gD-1 protein, leading to the expression of the recombinant protein on the surface of the transfected cells. The location of the recombinant protein was confirmed with transfected HEK-293 cellsby immunofluorescence assays. Mice immunized with the vaccine generated antigen-specific antibody responses after three doses administered intramuscularly (i.m), intradermally with a syringe (i.d) or intradermally by gene gun (bioballistic) (gg). The immunological responses mediated by specific-T CD8+ p24 lymphocytes were evaluated and, according to the data obtained, the i.m administration was chosen for the next assays. Aiming the improvement of the vaccine immunogenicity, particularly for cellular responses, the effect of co-administration with other plasmids was assessed with: plasmids that express cytokines (pIL-2, pIL-12 or pGM-CSF); a vaccine vector based on pVAX (pgDp24); and a vector encoding a synthetic gene capable to increase the expression of gD protein in mammalian cells (pgDhp24). Finally, we developed a murine model for the functional evaluation of antigen-specific cytotoxic responses using a tumor cell line which expresses the p24 protein. Balb/c mice, immunized with pgDp24 had a reduced tumor growth when compared to non-immunized mice. In addition, vaccinated mice showed partial protection to a lethal challenge with HSV-1.
|
5 |
Construção e caracterização imunológica de formulação vacinal com propriedade profiláticas voltada para o controle do vírus da imunodeficiência humana (HIV) e do vírus herpes (HSV). / Construction and immunological characterization of a vaccine formulation with prophylactic properties aiming to control the human immunodeficiency virus (HIV) and herpes virus (HSV).Francisco André Marques de Oliveira Cariri 13 August 2014 (has links)
O presente projeto propõe a avaliação de respostas imunológicas induzidas por uma vacina de DNA bivalente para o controle de infecções por HIV e HSV. A vacina genética codifica a proteína gD do vírus HSV-1 geneticamente fusionada à proteína Gag (p24) do HIV, rica em epítopos reconhecidos por células T CD8+. A vacina de DNA pRE4p24 codifica a proteína p24 inserida em região próxima à porção C-terminal da proteína gD-1, permitindo que a proteína recombinante seja expressa na superfície da célula transfectada. A localização da proteína recombinante foi confirmada na superfície das células HEK-293T transfectadas por ensaios de imunofluorescência. Camundongos imunizados com a vacina foram capazes de gerar respostas de anticorpos específicos após três doses administradas pelas vias intramuscular (i.m.), intradérmica com seringa (i.d.) ou intradérmico por biobalística (gg). Foram analisadas as respostas imunológicas mediadas por linfócitos T CD8+ p24-específicas e, em função dos resultados obtidos, sendo a via i.m. escolhida como a mais promissora para os ensaios subsequentes. Na tentativa de aumentar a imunogenicidade da vacina, particularmente, para respostas celulares, foi avaliado o efeito da co-administração da formulação vacinal com outros plasmídeos que expressam citocinas (pIL-2, pIL-12 ou pGM-CSF), o teste de um vetor vacinal baseado no plasmídeo multicópia pVAX (pgDp24) e o emprego de um gene sintético para promover o aumento da expressão da proteína gD em células de mamíferos (pgDhp24). Por fim, desenvolvemos um modelo murino para a avaliação funcional de respostas citotóxicas antígeno-específicas a partir de uma linhagem tumoral capaz de expressar a proteína p24 do HIV. Camundongos Balb/c imunizados com o pgDp24 apresentaram um retardo no crescimento tumoral em relação aos animais não imunizados além de proteção parcial a desafios letais com HSV-1. / The present thesis aims to evaluate the immunological responses induced by a bivalent DNA vaccine to the control HIV and HSV infectious. This genetic vaccine codes the gD protein from the HSV-1 virus envelope genetically fused with HIV Gag (p24) protein, which has various epitopes recognized by human and murine T CD8+ cells. The DNA vaccine, named pRE4p24, codes for p24 protein, inserted close to the C-terminal region of gD-1 protein, leading to the expression of the recombinant protein on the surface of the transfected cells. The location of the recombinant protein was confirmed with transfected HEK-293 cellsby immunofluorescence assays. Mice immunized with the vaccine generated antigen-specific antibody responses after three doses administered intramuscularly (i.m), intradermally with a syringe (i.d) or intradermally by gene gun (bioballistic) (gg). The immunological responses mediated by specific-T CD8+ p24 lymphocytes were evaluated and, according to the data obtained, the i.m administration was chosen for the next assays. Aiming the improvement of the vaccine immunogenicity, particularly for cellular responses, the effect of co-administration with other plasmids was assessed with: plasmids that express cytokines (pIL-2, pIL-12 or pGM-CSF); a vaccine vector based on pVAX (pgDp24); and a vector encoding a synthetic gene capable to increase the expression of gD protein in mammalian cells (pgDhp24). Finally, we developed a murine model for the functional evaluation of antigen-specific cytotoxic responses using a tumor cell line which expresses the p24 protein. Balb/c mice, immunized with pgDp24 had a reduced tumor growth when compared to non-immunized mice. In addition, vaccinated mice showed partial protection to a lethal challenge with HSV-1.
|
6 |
Filmes de Langmuir e vesículas multilamelares de fosfolipídios e suas interações com um peptídeo oriundo da proteína p24 do HIV-1 / Langmuir films and multilamellar vesicles of phospholipids and its interactions with peptide from p24 protein from HIV-1Moraes, Marli Leite de 03 October 2003 (has links)
A investigação dos mecanismos de interação dos vírus com as células do hospedeiro trazem informações relevantes para a identificação de alvos no desenvolvimento de drogas para impedir a penetração e/ou desenvolvimento dos vírus. Peptídeos desenhados a partir de proteínas virais foram desenvolvidos e testados quanto as suas capacidades de inibir o processo de fusão do vírus com a célula do hospedeiro. Alguns se encontram em fase de avaliação clínica. Anticorpos contra a proteína p24 do HIV-1 foram detectados no soro de pacientes HIV-positivos, e estes reconhecem pequenas seqüências peptídicas desta proteína. Neste trabalho foi analisada a interação entre uma seqüência peptídica correspondente aos aminoácidos 196-224 (AAMQMLKETINEEAAEWDRVHPVHAGPIA) da proteína p24, denominado p24- 1, com sistemas biomiméticos. Os sistemas utilizados foram filmes de Langmuir (monocamadas) de dipalmitoil fosfatidil colina (DPPC) e dipalmitoil fosfatidil glicerol (DPPG) e vesículas multilamelares (MLVs) de DPPC. O p24-1 encontra-se desorganizado em solução aquosa, mas com a interação com as MLVs de DPPC teve induzido uma conformação hélice ?, de acordo com o espectro de dicroísmo circular (CD). Esta característica foi confirmada pela predição de hélice a seguida por uma estrutura não ordenada contendo 11 resíduos do p24-1. As isotermas de pressão e potencial de superfície das monocamadas de DPPC foram afetadas com a presença de 0,05% mo1 de p24-1, com uma expansão de aproximadamente 5%. Para concentrações acima de 0,5% mo1 de p24-1 a expansão foi de 20%, com saturação do efeito da concentração. O efeito de expansão foi acompanhado por uma alteração na morfologia das monocamadas, estudados com microscopia no ângulo de Brewster (BAM). A incorporação do p24-1 impede a formação de grandes domínios de DPPC. O efeito cooperativo causado na monocamada de fosfolipídios pelo p24-1 sugere que esse tem um potencial na atividade antiviral por participar da expansão da membrana da célula hospedeira. / The investigation of the interaction mechanisms between the viruses and the host cells brings relevant information for the identification of targets on the development of drugs to prevent the penetration and/or development of the viruses. Peptides designed from viral proteins have been developed and tested on its capacities of inhibiting the merging process of the virus with the host cell. Some of them are in clinical evaluation. Antibodies against the protein p24 of the HIV-1 have been detected in the serum of HIV-positive patients, and they are able to recognize short peptide sequences of this protein. In this work, it was analyzed the interaction between a peptide sequence corresponding to amino acids 196-224 (AAMQMLKETINEEAAEWDRVHPVHAGPIA) of the protein p24, called p24- 1, and biomimetic systems. The systems used were Langmuir films (monolayers) of dipalmitoyl phosphatidyl choline (DPPC) and dipalmitoyl phosphatidyl glycerol (DPPG) and multilamelar vesicles (MLVs) of DPPC. p24-1 is found disorganized in watery solution, but with the interaction with the MLVs of DPPC it had induced a conformation ?-helix, according to the circular dichoism spectra (CD). This characteristic was confirmed by the prediction of ?-helix followed by an unordered structure with 11 residues of p24-1. The isotherms of pressure and potential of surface of the DPPC monolayers were affected by the presence of 0,05% mo1 of p24-1, with an expansion of approximately 5%. For concentrations above 0,5% mol of p24-1 the expansion was 20%, with saturation of the concentration effect. The expansion effect was followed by a morphologic alteration of the monolayers, studied with microscopy of the Brewster angle (BAM). The incorporation of p24-1 prevents the formation of large domains of DPPC. The cooperative effect caused in the phospholipid monolayer by p24-1 suggests that this peptide has a potential in the antiviral activity, once its participates on the expansion of the host cell membrane.
|
7 |
Filmes de Langmuir e vesículas multilamelares de fosfolipídios e suas interações com um peptídeo oriundo da proteína p24 do HIV-1 / Langmuir films and multilamellar vesicles of phospholipids and its interactions with peptide from p24 protein from HIV-1Marli Leite de Moraes 03 October 2003 (has links)
A investigação dos mecanismos de interação dos vírus com as células do hospedeiro trazem informações relevantes para a identificação de alvos no desenvolvimento de drogas para impedir a penetração e/ou desenvolvimento dos vírus. Peptídeos desenhados a partir de proteínas virais foram desenvolvidos e testados quanto as suas capacidades de inibir o processo de fusão do vírus com a célula do hospedeiro. Alguns se encontram em fase de avaliação clínica. Anticorpos contra a proteína p24 do HIV-1 foram detectados no soro de pacientes HIV-positivos, e estes reconhecem pequenas seqüências peptídicas desta proteína. Neste trabalho foi analisada a interação entre uma seqüência peptídica correspondente aos aminoácidos 196-224 (AAMQMLKETINEEAAEWDRVHPVHAGPIA) da proteína p24, denominado p24- 1, com sistemas biomiméticos. Os sistemas utilizados foram filmes de Langmuir (monocamadas) de dipalmitoil fosfatidil colina (DPPC) e dipalmitoil fosfatidil glicerol (DPPG) e vesículas multilamelares (MLVs) de DPPC. O p24-1 encontra-se desorganizado em solução aquosa, mas com a interação com as MLVs de DPPC teve induzido uma conformação hélice ?, de acordo com o espectro de dicroísmo circular (CD). Esta característica foi confirmada pela predição de hélice a seguida por uma estrutura não ordenada contendo 11 resíduos do p24-1. As isotermas de pressão e potencial de superfície das monocamadas de DPPC foram afetadas com a presença de 0,05% mo1 de p24-1, com uma expansão de aproximadamente 5%. Para concentrações acima de 0,5% mo1 de p24-1 a expansão foi de 20%, com saturação do efeito da concentração. O efeito de expansão foi acompanhado por uma alteração na morfologia das monocamadas, estudados com microscopia no ângulo de Brewster (BAM). A incorporação do p24-1 impede a formação de grandes domínios de DPPC. O efeito cooperativo causado na monocamada de fosfolipídios pelo p24-1 sugere que esse tem um potencial na atividade antiviral por participar da expansão da membrana da célula hospedeira. / The investigation of the interaction mechanisms between the viruses and the host cells brings relevant information for the identification of targets on the development of drugs to prevent the penetration and/or development of the viruses. Peptides designed from viral proteins have been developed and tested on its capacities of inhibiting the merging process of the virus with the host cell. Some of them are in clinical evaluation. Antibodies against the protein p24 of the HIV-1 have been detected in the serum of HIV-positive patients, and they are able to recognize short peptide sequences of this protein. In this work, it was analyzed the interaction between a peptide sequence corresponding to amino acids 196-224 (AAMQMLKETINEEAAEWDRVHPVHAGPIA) of the protein p24, called p24- 1, and biomimetic systems. The systems used were Langmuir films (monolayers) of dipalmitoyl phosphatidyl choline (DPPC) and dipalmitoyl phosphatidyl glycerol (DPPG) and multilamelar vesicles (MLVs) of DPPC. p24-1 is found disorganized in watery solution, but with the interaction with the MLVs of DPPC it had induced a conformation ?-helix, according to the circular dichoism spectra (CD). This characteristic was confirmed by the prediction of ?-helix followed by an unordered structure with 11 residues of p24-1. The isotherms of pressure and potential of surface of the DPPC monolayers were affected by the presence of 0,05% mo1 of p24-1, with an expansion of approximately 5%. For concentrations above 0,5% mol of p24-1 the expansion was 20%, with saturation of the concentration effect. The expansion effect was followed by a morphologic alteration of the monolayers, studied with microscopy of the Brewster angle (BAM). The incorporation of p24-1 prevents the formation of large domains of DPPC. The cooperative effect caused in the phospholipid monolayer by p24-1 suggests that this peptide has a potential in the antiviral activity, once its participates on the expansion of the host cell membrane.
|
8 |
Evaluation and validation of in vitro assays to determine cell viability for HIV/AIDS expermentation with Pheroid TM technology / Helanie van der Merwe.Van der Merwe, Helanie January 2008 (has links)
The Southern parts of Africa have the highest prevalence of HIV-infected people and
South Africa is the country with the highest number of infections in the world. There
is still no cure for AIDS, but anti-HIV medicine can prolong and enhance the quality of
life of an HIV infected person. Patient adherence with antiretroviral therapy is
extremely low due to difficult dosing intervals, problematic dosage forms, instability of
the antiretrovirals (ARVs) and the severe side-effects caused by these drugs; this
leads to resistance of HIV to these drugs.
Pheroid™ technology is a patented delivery system. Pheroid™ vesicles were used
during this study. The entrapment of an active within the Pheroid™ would generally
provide a safer, more effective formulation than the active alone. This could mean
that the amount of drug needed for treatment of HIV can be decreased while
producing fewer adverse effects and reducing the price of treatment.
The main objectives of this study were to optimise and validate the cell viability and
viral replication assays that can be used in an in vitro viral infection model. The MTT
assay was used to asses the viability of the cells and to determine the toxicity of the
antiretroviral drugs and Pheroid™ on the cells. HIV-1 assays were evaluated and
used to determine the viral replication in the cells.
Two different continuous cell lines were chosen for this study, an anchorage
dependent GHOST cell line and suspended M7-Luc cells. Both these cell lines were
best infected with the SWl virus. SWl is a subtype C, CXCR4 utilising virus.
Subtype C is responsible for 60 % of the HIV infections worldwide and is the
prevalent subtype in SUb-Saharan Africa .. Infection enhancers were not added to the
cells to improve viral infection since it was observed that the Pheroid™ in
combination with DEAE-dextran or Polybrene caused cytotoxicity probably by
disrupting the cell's membrane. Antioxidants were added to the Pheroid ™
formulation since it was observed that the viability of the cells incubated with the
Pheroid™ decreased as the Pheroid ™ matured. The added antioxidants had no
significant effect on the cells.
Abacavir (ABC) was chosen as the test substance for this study since it showed low
cytotoxicity in cell cultures and is water soluble and would not present solubility
issues in the media. It was entrapped within the Pheroid™ and its in vitro efficacy
and toxicity was tested on HIV-infected and uninfected cell cultures.
One directlHIV-specific (p24 antigen ELISA assay) and one indirect (Luciferase)
assays were used to asses the inhibition of HIV replication caused by ABC. The p24
antigen ELISA (Enzyme-Linked ImmunoSorbent Assay) assay required a lot of
washing steps and were rather expensive to use. The Luciferase assay was only
used on the M7-Luc cells; this assay was sensitive, inexpensive and easy to use.
The MTT (3-(4,5-demethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) viability
assay was used to measure the toxicity caused by the Pheroid ™ and/or ABC on the
cells. MTT is a widely used quantitative colorimetric assay to measure the viability of
cells. The vitamin E and antioxidants contained in the Pheroid ™ reduced the MTT
and produced results that were misinterpreted as enhanced viability when the
Pheroid™ was present during MTT analysis. To prevent this problem an additional
washing step should be introduced prior to analysis to reduce the interference of the
Pheroid ™ with analytical methods.
In conclusion, the efficacy of ABC entrapped within the Pheroid™ is still inconclusive
and further studies will have to be done. MTT should be used with care for viability
analysis of cells incubated in the presence of Pheroid TM. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2009.
|
9 |
Evaluation and validation of in vitro assays to determine cell viability for HIV/AIDS expermentation with Pheroid TM technology / Helanie van der Merwe.Van der Merwe, Helanie January 2008 (has links)
The Southern parts of Africa have the highest prevalence of HIV-infected people and
South Africa is the country with the highest number of infections in the world. There
is still no cure for AIDS, but anti-HIV medicine can prolong and enhance the quality of
life of an HIV infected person. Patient adherence with antiretroviral therapy is
extremely low due to difficult dosing intervals, problematic dosage forms, instability of
the antiretrovirals (ARVs) and the severe side-effects caused by these drugs; this
leads to resistance of HIV to these drugs.
Pheroid™ technology is a patented delivery system. Pheroid™ vesicles were used
during this study. The entrapment of an active within the Pheroid™ would generally
provide a safer, more effective formulation than the active alone. This could mean
that the amount of drug needed for treatment of HIV can be decreased while
producing fewer adverse effects and reducing the price of treatment.
The main objectives of this study were to optimise and validate the cell viability and
viral replication assays that can be used in an in vitro viral infection model. The MTT
assay was used to asses the viability of the cells and to determine the toxicity of the
antiretroviral drugs and Pheroid™ on the cells. HIV-1 assays were evaluated and
used to determine the viral replication in the cells.
Two different continuous cell lines were chosen for this study, an anchorage
dependent GHOST cell line and suspended M7-Luc cells. Both these cell lines were
best infected with the SWl virus. SWl is a subtype C, CXCR4 utilising virus.
Subtype C is responsible for 60 % of the HIV infections worldwide and is the
prevalent subtype in SUb-Saharan Africa .. Infection enhancers were not added to the
cells to improve viral infection since it was observed that the Pheroid™ in
combination with DEAE-dextran or Polybrene caused cytotoxicity probably by
disrupting the cell's membrane. Antioxidants were added to the Pheroid ™
formulation since it was observed that the viability of the cells incubated with the
Pheroid™ decreased as the Pheroid ™ matured. The added antioxidants had no
significant effect on the cells.
Abacavir (ABC) was chosen as the test substance for this study since it showed low
cytotoxicity in cell cultures and is water soluble and would not present solubility
issues in the media. It was entrapped within the Pheroid™ and its in vitro efficacy
and toxicity was tested on HIV-infected and uninfected cell cultures.
One directlHIV-specific (p24 antigen ELISA assay) and one indirect (Luciferase)
assays were used to asses the inhibition of HIV replication caused by ABC. The p24
antigen ELISA (Enzyme-Linked ImmunoSorbent Assay) assay required a lot of
washing steps and were rather expensive to use. The Luciferase assay was only
used on the M7-Luc cells; this assay was sensitive, inexpensive and easy to use.
The MTT (3-(4,5-demethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) viability
assay was used to measure the toxicity caused by the Pheroid ™ and/or ABC on the
cells. MTT is a widely used quantitative colorimetric assay to measure the viability of
cells. The vitamin E and antioxidants contained in the Pheroid ™ reduced the MTT
and produced results that were misinterpreted as enhanced viability when the
Pheroid™ was present during MTT analysis. To prevent this problem an additional
washing step should be introduced prior to analysis to reduce the interference of the
Pheroid ™ with analytical methods.
In conclusion, the efficacy of ABC entrapped within the Pheroid™ is still inconclusive
and further studies will have to be done. MTT should be used with care for viability
analysis of cells incubated in the presence of Pheroid TM. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2009.
|
10 |
Studies on HIV-1 core assembly /Abdurahman, Samir, January 2007 (has links)
Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007. / Härtill 4 uppsatser.
|
Page generated in 0.0373 seconds