Ce travail de thèse s'inscrit dans le contexte général des solutions envisageables pour répondre aux problèmes du réchauffement climatique global et de la diminution des ressources fossiles. Il a été en majeure partie consacré à l'étude et au développement d'électrocatalyseurs moléculaires pour la réduction du dioxyde de carbone. Un tel procédé pourrait permettre de transformer et valoriser le CO2 en l'exploitant comme une matière première carbonée disponible en quantité non limitée, bon marché et peu toxique. Dans une première partie, un métallocavitand, dont la structure est basée sur un calix[4]arène fonctionnalisé par quatre complexes phénanthroline tris-carbonyl de rhénium(I), a été étudié en solution et sur déposé sur surface transparente conductrice. Le but de cette étude était de contrôler par un stimulus électrochimique la fermeture et l'ouverture du bord large du cavitand. La complexité des processus rédox de ces métallo-macrocycles nous a conduits à étudier en détails les propriétés électrochimiques de complexes de rhénium(I) modèles. Ces propriétés avaient précédemment été rapportées dans la littérature mais étaient sujettes à désaccords entre les différents auteurs. Finalement l'ensemble de cette étude nous a conduits à préciser et à mieux comprendre le mécanisme de la catalyse de réduction du CO2 par ce type de complexes. Dans une deuxième partie, qui représente la partie la plus conséquente de cette thèse, la synthèse, la caractérisation physico-chimique et l'étude spectro-électrochimique de complexes bipyridine tris-carbonyle de manganèse, analogues des complexes de rhénium évoqués ci-dessus, ont été entreprises. Le but était de développer un catalyseur innovant pour la réduction électrochimique du dioxyde de carbone, du type complexe métallique, en substituant les métaux rares utilisés actuellement par un métal non-noble et abondant : le manganèse. Les propriétés électrochimiques des nouveaux complexes de Mn synthétisés ont été caractérisées en détail. La simulation des voltammétries cycliques, associée à des études mécanistiques par spectro-électrochimie UV-vis, a permis d'accéder aux données cinétiques et thermodynamiques des différentes réactions chimiques associées aux réactions de transferts d'électron irréversibles. Ces complexes de manganèse se sont révélés être des électrocatalyseurs de la réduction du CO2 au moins aussi efficaces que les complexes analogues de rhénium, décrits jusque-là comme étant les meilleurs systèmes pour cette application. Les complexes de Mn ainsi préparés montrent une excellente sélectivité (rendements faradiques élevés et peu ou pas de H2) et une bonne efficacité pour l'électro-réduction du CO2 en CO en milieu hydro-organique. Les catalyses à l'échelle préparatrice fonctionnent avec des surtensions modérées. Les mécanismes mis en jeu lors de la catalyse ont été étudiés par spectro-électrochimie UV-vis et RPE (en onde continue et pulsée) associées à des caculs théoriques DFT. Un intermédiaire-clé a ainsi pu être mis en évidence et caractérisé. Enfin, l'étude des réactions de décarbonylation photo-induites dans ces complexes est rapportée. / This PhD takes place in the general context of possible solutions to global warming and peak oil issues. It mostly deals with the study and development of molecular electrocatalysts for carbon dioxide reduction. This process may open the way to the use and valorization of CO2 as a raw material. In the first part, a metallocavitand, which structure is based on a calix[4]arene modified by four phenanthroline tris-carbonyl rhenium(I) complexes, was studied in solution and deposited on a conductive transparent surface. The aim of this study was to control through an electrochemical stimulus the closing and opening of the wide rim of the cavitand. Complexity of the redox properties of the metallocavitand led us to investigate in detail the electrochemical properties of simpler examples of these rhenium(I) complexes. These properties were previously described in the literature ; however, there was disagreement in the interpretation of the results. Finally this study led us to a more precise and better understanding of the mechanism of the reduction of CO2 by a complex of this kind. In the second part, which is the main part of this PhD, synthesis, physico-chemical characterization and spectro-electrochemical study of bipyridine tris-carbonyl manganese complexes, analogues of the rhenium complexes mentioned above, were undertaken. The aim was to develop an innovative catalyst for the electrochemical reduction of carbon dioxide, based on a metallic complex, by substituting rare and noble metals currently used by a non-noble and abundant metal: manganese. Electrochemical properties of newly synthesized manganese complexes were investigated in detail. Simulations of cyclic voltammetry, associated with mechanism studies by UV-vis spectro-electrochemistry, allowed us to determine kinetic and thermodynamic properties of the different chemical reactions coupled with the irreversible electron transfers. These manganese complexes proved to electrocatalyse the reduction of carbon dioxide at least as efficiently as the analog rhenium complexes which were, until now, the best system for this application. These manganese electrocatalysts exhibit a very good selectivity (high faradic yields and no or very little H2) and a good efficiency for reduction of CO2 to CO in aqueous-organic media. Preparative scale electrolysis operates at low overpotential. Mechanisms of the different catalytic pathways were studied using UV-vis and EPR (continuous wave or pulsed) spectro-electrochemistry associated with DFT calculations. A key-intermediate was evinced and investigated. Finally the study of photo-induced decarbonylation reactions of these complexes is also described.

L'origine de champs magnétiques observés dans les plasmas de laboratoire et d'astrophysique est l'un des problèmes récurrents en physique des plasmas. À cet égard, les instabilités de type Weibel sont considérées d'une grande importance. Ces instabilités ont pour origine une anisotropie de température (instabilité de Weibel) et des moments des électrons (instabilité de filamentation de courant). L'objectif principal de cette thèse est l'étude théorique et numérique de ces instabilités dans un plasma non collisionnel en régime relativiste. Le premier aspect de ce travail est l'étude du régime non-linéaire de ces instabilités et du rôle des effets cinétiques et relativistes sur la structure des champs électromagnétiques auto-cohérents. Dans ce cadre, un problème essentiel pour les applications et la théorie, concerne l'identification et l'analyse des structures cohérentes développées spontanément dans le régime non-linéaire sur des échelles cinétiques. Un deuxième aspect du travail est le développement de techniques analytiques et numériques pour l'étude des plasmas non collisionnels. Le modèle mathématique de référence, à la base des études des plasmas chauds, est le modèle Vlasov-Maxwell, où l'équation de Vlasov (théorie des champs moyens) est couplée aux équations de Maxwell de façon auto-cohérente. Un modèle unidimensionnel, le modèle multi-faisceaux, a également été introduit durant cette thèse. Basé sur une technique de réduction en dimension, il est à la fois un modèle analytique "simple" présentant l'avantage de pouvoir résoudre une équation de Vlasov 1D pour chaque faisceau de particules, et un modèle numérique moins coûteux qu'un modèle complet / The origin of magnetic fields observed in laboratory and astrophysical plasmas is one ofthe most challenging problems in plasma physics. In this respect, the Weibel type instabilities are considered of key importance. These instabilities are caused by a temperature anisotropy (Weibel instability) and electron momentum (current filamentation instability). The main objective of this thesis is the theoretical and numerical study of these instabilities in a collisionless plasma in the relativistic regime. The first aspect of this work is to study the nonlinear regime of these instabilities and the role of kinetic and relativistic effects on the structure of self-consistent electromagnetic fields. In this context, a key problem for the theory and applications, is the identification and analysis of coherent structures developed spontaneously in the nonlinear regime of kinetic scales. A second aspect of the work is the development of analytical and numerical techniques for the study of collisionless plasmas. A mathematical model of reference is the Vlasov-Maxwell model, where the Vlasov equation (mean field theory) is coupled to the Maxwell equations in a self-consistent way. A one-dimensional model, the multi-stream model, is also introduced. Based on a dimensional reduction technique, it is both an analytical model "simple" having the advantage of being able to solve a 1D Vlasov equation for each particle beam, and a numerical model less expensive than a complete model

Cette thèse s’intéresse à l’étude d’une structure de moteur électrique à aimants permanents afin de réduire l’utilisation d’aimants à basse de terres rares et qui puisse être utilisée pour des applications industrielles. Il est montré dans la première partie de ce travail de recherche que la machine synchro-réluctante à aimants permanents est une bonne solution potentielle. Une analyse paramétrique est alors réalisée en utilisant une modélisation par éléments finis pour mettre en évidence les particularités de son comportement électromagnétique. Puis, une modélisation analytique multi-physique innovante du système convertisseur-moteur est détaillée dans le but de calculer les performances de ce dernier en un temps raisonnable. Les modèles multi-physiques présentés dans ces travaux concernent l’onduleur et le moteur. Ils intègrent les aspects électromagnétique, électrique, énergétique, thermique, mécanique et technico-économique. Le modèle multi-physique de la machine électrique est validé par comparaison à des résultats d’essais sur un prototype. Le modèle du système qui a été développé est ensuite utilisé dans une procédure de conception par optimisation de systèmes d’entrainements. Pour cela, une démarche d’optimisation originale est présentée pour le dimensionnement conjoint de deux applications en imposant la contrainte d’utiliser la même tôlerie magnétique. Il s’agit d’une part d’une application à vitesse fixe et d’autre part d’une application de type traction électrique. La méthode d’optimisation employée est à évolution différentielle. Les résultats des optimisations réalisées permettent de déterminer des conceptions optimales ou des compromis optimaux aux sens de Pareto qui répondent aux deux applications visées. Finalement, cette thèse a permis de positionner la machine synchro-réluctante à aimants permanents parmi les structures de machines à fort potentiel industriel. / This thesis focuses on the study of a structure of permanent magnet electric motor which reduces the amount used of permanent magnets composed of rare earths and which can be used in industrial applications. In the first part of the research work, it is shown that the permanent magnet assisted synchronous reluctance machine is a good alternative. A parametric analyse is realised using a finite element modelling in order to highlight the peculiarities of its electromagnetic behaviour. Then, an innovative multi-physic analytical modelling for the system inverter-motor is detailed in order to evaluate its performances in a reasonable computational time. The multi-physic models presented in this work concern the inverter and motor. They integrate the electromagnetic, electric, energetic, thermal, mechanic, and techno-economic aspects. The multi-physical model of the electric machine is validated by means of tests carried out on a prototype. The model of the system which has been developed is used in a design procedure by optimization of drive systems. For this purpose, an original optimization approach is presented for the simultaneous design of two applications by imposing the constraint of using the same magnetic lamination. On one hand it is an application of fixed speed and on the other hand an application of electric traction. The optimization method used is a type of differential evolution optimization. The results of the optimizations realised determine the optimal designs or the optimal compromise with Pareto front which deal with both applications. Finally, this thesis has placed the permanent magnet assisted synchronous reluctance machine among structures of machines with great industrial potential.

L'objectif de ce travail était d'étudier les interactions entre une plante à phytoremédiatrice ?, Lantana camara (Verbenaceae), le ver de terre, Pontoscolex corethrurus (Glossoscolecidae) et les microorganismes telluriques d'un sol pollué au plomb. Dans un premier temps, il apparaît que dans les sols contaminés, la présence de ver conduit à un accroissement de la biomasse des parties aériennes et racinaires des plantes ainsi qu'à une augmentation de l'absorption de plomb. La caractérisation physico-chimique des agrégats racinaires a montré que l'activité des vers augmente le taux de matière organique, la capacité d'échange cationique ainsi que l'azote total, le potassium total et disponible. De plus, la présence des vers augmente certaines activités enzymatiques de la rhizosphère. La croissance accrue de L. camara pourrait résulter de ces différentes actions. L'action des vers de terre sur les plantes se ferait via les communautés microbiennes telluriques. Ainsi, la biomasse des microorganismes, bactéries et champignons, des agrégats racinaires augmente en présence de vers. La PCR-DGGE n'a pas permis de mettre en évidence de modifications de la structure taxonomique des communautés bactériennes sous l'influence du Pb et/ou du vers, par contre l'analyse des profils physiologiques par plaques Biolog montre clairement une diversification fonctionnelle bactérienne. Les communautés fongiques voient, elles, leur diversité taxonomique, augmenter sous l'action des vers. La restructuration des populations microbiennes, en présence de vers, des agrégats racinaires élaborés par les plantes en milieu pollué au plomb est l'élément déterminant pour la compréhension de l'impact de P. corethrurus sur la croissance et la phytoremédiation de L. camara. L'association de ces deux organismes aurait donc un potentiel considérable pour le traitement de sites industriels pollués au plomb / The objective of this work was to study the interactions between phytoremediating plant Lantana camara (Verbenaceae), the earthworm Pontoscolex corethrurus (Glossocolecidae) and microorganisms in soil contaminated with lead. Initially, it appears that in the contaminated soil, the presence of earthworm leads to an increase in the biomass of root and aerial parts of plants and increased absorption of lead. The physico-chemical characterization of root-aggregates showed that the activity of earthworms increases the rate of organic matter, cation exchange capacity, total nitrogen, total and available potassium. Moreover, the presence of earthworms increases certain enzymatic activities in the rhizosphere. The increased growth of L. camara could result from these different actions. The action of earthworm on plants would be through terrestrial microbial-communities. Thus, the biomass of microorganisms, bacteria and fungi, of root-aggregates increase in the presence of earthworms. By PCR-DGGE, we were unable to demonstrate differences in taxonomic diversity of the bacteria community but the analysis of physiological profiles with Biolog plates showed that the activities of earthworm enhance the functional diversity of soil bacteria. In other hand, the restructuring of fungal taxonomy has been clearly observed by the activity of earthworm. All changes observed can explain increased growth of plants and improved phytoextraction of heavy metal. Finally, the study underlines the role of the earthworms on the growth and the phytoextraction efficiency of the plants. So, the combination of earthworm P. corethrurus and plant L. camara could be considerable potential for the treatment of industrial sites polluted with lead

Cette thèse s’intéresse à l’étude d’une structure de moteur électrique à aimants permanents afin de réduire l’utilisation d’aimants à basse de terres rares et qui puisse être utilisée pour des applications industrielles. Il est montré dans la première partie de ce travail de recherche que la machine synchro-réluctante à aimants permanents est une bonne solution potentielle. Une analyse paramétrique est alors réalisée en utilisant une modélisation par éléments finis pour mettre en évidence les particularités de son comportement électromagnétique. Puis, une modélisation analytique multi-physique innovante du système convertisseur-moteur est détaillée dans le but de calculer les performances de ce dernier en un temps raisonnable. Les modèles multi-physiques présentés dans ces travaux concernent l’onduleur et le moteur. Ils intègrent les aspects électromagnétique, électrique, énergétique, thermique, mécanique et technico-économique. Le modèle multi-physique de la machine électrique est validé par comparaison à des résultats d’essais sur un prototype. Le modèle du système qui a été développé est ensuite utilisé dans une procédure de conception par optimisation de systèmes d’entrainements. Pour cela, une démarche d’optimisation originale est présentée pour le dimensionnement conjoint de deux applications en imposant la contrainte d’utiliser la même tôlerie magnétique. Il s’agit d’une part d’une application à vitesse fixe et d’autre part d’une application de type traction électrique. La méthode d’optimisation employée est à évolution différentielle. Les résultats des optimisations réalisées permettent de déterminer des conceptions optimales ou des compromis optimaux aux sens de Pareto qui répondent aux deux applications visées. Finalement, cette thèse a permis de positionner la machine synchro-réluctante à aimants permanents parmi les structures de machines à fort potentiel industriel. / This thesis focuses on the study of a structure of permanent magnet electric motor which reduces the amount used of permanent magnets composed of rare earths and which can be used in industrial applications. In the first part of the research work, it is shown that the permanent magnet assisted synchronous reluctance machine is a good alternative. A parametric analyse is realised using a finite element modelling in order to highlight the peculiarities of its electromagnetic behaviour. Then, an innovative multi-physic analytical modelling for the system inverter-motor is detailed in order to evaluate its performances in a reasonable computational time. The multi-physic models presented in this work concern the inverter and motor. They integrate the electromagnetic, electric, energetic, thermal, mechanic, and techno-economic aspects. The multi-physical model of the electric machine is validated by means of tests carried out on a prototype. The model of the system which has been developed is used in a design procedure by optimization of drive systems. For this purpose, an original optimization approach is presented for the simultaneous design of two applications by imposing the constraint of using the same magnetic lamination. On one hand it is an application of fixed speed and on the other hand an application of electric traction. The optimization method used is a type of differential evolution optimization. The results of the optimizations realised determine the optimal designs or the optimal compromise with Pareto front which deal with both applications. Finally, this thesis has placed the permanent magnet assisted synchronous reluctance machine among structures of machines with great industrial potential.

Depuis la fermeture de Phénix en 2010 le CEA ne possède plus de réacteur au sodium. Vus les enjeux énergétiques et le potentiel de la filière, le CEA a lancé un programme de démonstrateur industriel appelé ASTRID (Advanced Sodium Technological Reactor for Industrial Demonstration), réacteur d’une puissance de 600MW électriques (1500 MW thermiques). L’objectif du prototype est double, être une réponse aux contraintes environnementales et démontrer la viabilité industrielle :• De la filière RNR-Na, avec un niveau de sureté au moins équivalent aux réacteurs de 3ème génération, du type de l’EPR. ASTRID intégrera dès la conception le retour d’expérience de Fukushima ;• Du retraitement des déchets (transmutation d’actinide mineur) et de la filière qui lui serait liée.La sûreté de l’installation est prioritaire, aucun radioélément ne doit être rejeté dans l’environnement, et ce dans toutes les situations. Pour atteindre cet objectif, il est impératif d’anticiper l’impact des nombreuses sources d’incertitudes sur le comportement du réacteur et ce dès la phase de conception. C’est dans ce contexte que s’inscrit cette thèse dont l’ambition est le développement de nouvelles méthodes d’optimisation des cœurs des RNR-Na. L’objectif est d’améliorer la robustesse et la fiabilité des réacteurs en réponse à des incertitudes existantes. Une illustration sera proposée à partir des incertitudes associées à certains régimes transitoires dimensionnant. Nous utiliserons le modèle ASTRID comme référence pour évaluer l’intérêt des nouvelles méthodes et outils développés.L’impact des incertitudes multi-Physiques sur le calcul des performances d’un cœur de RNR-Na et l’utilisation de méthodes d’optimisation introduisent de nouvelles problématiques :• Comment optimiser des cœurs « complexes » (i.e associés à des espaces de conception de dimensions élevée avec plus de 20 paramètres variables) en prenant en compte les incertitudes ?• Comment se comportent les incertitudes sur les cœurs optimisés par rapport au cœur de référence ?• En prenant en compte les incertitudes, les réacteurs sont-Ils toujours considérés comme performants ?• Les gains des optimisations obtenus à l’issue d’optimisations complexes sont-Ils supérieurs aux marges d’incertitudes (qui elles-Mêmes dépendent de l’espace paramétrique) ?La thèse contribue au développement et à la mise en place des méthodes nécessaires à la prise en compte des incertitudes dans les outils de simulation de nouvelle génération. Des méthodes statistiques pour garantir la cohérence des schémas de calculs multi-Physiques complexes sont également détaillées.En proposant de premières images de cœur de RNR-Na innovants, cette thèse présente des méthodes et des outils permettant de réduire les incertitudes sur certaines performances des réacteurs tout en les optimisant. Ces gains sont obtenus grâce à l’utilisation d’algorithmes d’optimisation multi-Objectifs. Ces méthodes permettent d’obtenir tous les compromis possibles entre les différents critères d’optimisations comme, par exemple, les compromis entre performance économique et sûreté. / Since Phenix shutting down in 2010, CEA does not have Sodium Fast Reactor (SFR) in operating condition. According to global energetic challenge and fast reactor abilities, CEA launched a program of industrial demonstrator called ASTRID (Advanced Sodium Technological Reactor for Industrial Demonstration), a reactor with electric power capacity equal to 600MW. Objective of the prototype is, in first to be a response to environmental constraints, in second demonstrates the industrial viability of:• SFR reactor. The goal is to have a safety level at least equal to 3rd generation reactors. ASTRID design integrates Fukushima feedback;• Waste reprocessing (with minor actinide transmutation) and it linked industry.Installation safety is the priority. In all cases, no radionuclide should be released into environment. To achieve this objective, it is imperative to predict the impact of uncertainty sources on reactor behaviour. In this context, this thesis aims to develop new optimization methods for SFR cores. The goal is to improve the robustness and reliability of reactors in response to existing uncertainties. We will use ASTRID core as reference to estimate interest of new methods and tools developed.The impact of multi-Physics uncertainties in the calculation of the core performance and the use of optimization methods introduce new problems:• How to optimize “complex” cores (i.e. associated with design spaces of high dimensions with more than 20 variable parameters), taking into account the uncertainties?• What is uncertainties behaviour for optimization core compare to reference core?• Taking into account uncertainties, optimization core are they still competitive? Optimizations improvements are higher than uncertainty margins?The thesis helps to develop and implement methods necessary to take into account uncertainties in the new generation of simulation tools. Statistical methods to ensure consistency of complex multi-Physics simulation results are also detailed.By providing first images of innovative SFR core, this thesis presents methods and tools to reduce the uncertainties on some performance while optimizing them. These gains are achieved through the use of multi-Objective optimization algorithms. These methods provide all possible compromise between the different optimization criteria, such as the balance between economic performance and safety.

Στα πλαίσια αυτής της διδακτορικής εργασίας μελετήθηκαν οι εμπορικά σημαντικότερες περιοχές καλλιέργειας και συγκομιδής οστρακοειδών του Ελλαδικού χώρου. Κατά τη διάρκεια περιόδου 18 μηνών πραγματοποιήθηκε μηνιαία συλλογή δειγμάτων στρειδιών (Οstrea edulis) και μυδιών (Mytilus galloprovincialis), τα οποία συλλέχθηκαν από έξι (6) διαφορετικά σημεία του Ελλαδικού χώρου και αναλύθηκαν για τους εντεροϊούς (EV), τους αδενοϊούς (Adv), τον ιό της ηπατίτιδας Α (HAV), τους ιούς Noro I και II (NLVI και NLVII), για το βακτήριο Ε. coli, καθώς και για σωματικούς κολιφάγους, τους F-sperific RNA βακτηριοφάγους και τους βακτηριοφάγους του Β. fragilis. Επιπλέον αναπτύχθηκαν μέθοδοι τόσο για την ανίχνευση παθογόνων ιών ανθρώπινης προέλευσης στα οστρακοειδή, όσο και για την ανίχνευση των "πιθανών δεικτών" αυτών των ιών. Οι μέθοδοι εξετάστηκαν προκειμένου να αξιολογηθεί η απόδοση καλής ποιότητας από όλα τα εργαστήρια μέσω διεργαστηριακών αναλύσεων. Η μέθοδος που εφαρμόστηκε σε αυτή τη μελέτη για την ανίχνευση των ιών στα οστρακοειδή βασίζεται στην εξαγωγή και την ομογενοποίηση του πεπτικού αδένα με χρήση διαλύματος γλυκίνης, pH 10, απομόνωση των νουκλεϊνικών οξέων και ενίσχυση του γονιδιώματος των ιών που αναλύονται. Για την ανίχνευση του βακτηρίου E. coli χρησιμοποιήθηκε η μέθοδος των πολλαπλών σωλήνων, ενώ για την ανίχνευση των βακτηριοφάγων χρησιμοποιήθηκε η μέθοδος καλλιέργειας διπλοστιβάδας. Για το βακτήριο E. coli, σε σύνολο 138 δειγμάτων, 110 δείγματα (ποσοστό 79,7%) βρέθηκαν να ανήκουν στην κατηγορία Α (MPN/100g σάρκας = <20 έως 220), δηλαδή χαρακτηρίζονται σαν δείγματα χαμηλής μόλυνσης, 25 δείγματα (ποσοστό 18,1%) βρέθηκαν να ανήκουν στην κατηγορία Β (MPN/100g σάρκας = 220 έως 3500), οπότε χαρακτηρίζονται σαν δείγματα μεσαίας μόλυνσης, ακατάλληλα προς κατανάλωση χωρίς να προηγηθεί διαδικασία εξυγίανσης, ενώ μόνο 3 δείγματα (ποσοστό 2,2%) βρέθηκαν να ανήκουν στη κατηγορία C (MPN/100g σάρκας =3500 έως >18000), δηλαδή είναι δείγματα υψηλής μόλυνσης. Οι ιοί που εμφανίζονται με μεγαλύτερη συχνότητα στα οστρακοειδή της Ανατολικής Μεσογείου είναι οι αδενοϊοί (34% των δειγμάτων βρέθηκαν θετικά για τους αδενοϊούς) και ακολουθούν οι εντεροϊοί (16,7% των δειγμάτων βρέθηκαν θετικά για τους εντεροϊούς). Αντίθετα, ο ιός της ηπατίτιδας Α (ποσοστό θετικών δειγμάτων = 4,34%), καθώς και οι ιοί Noro I (ποσοστό θετικών δειγμάτων = 2,1%) και Noro II (ποσοστό θετικών δειγμάτων = 1,47%%) εμφανίζονται σε μικρό ποσοστό δειγμάτων. Τέλος, 80 δείγματα (58%) βρέθηκαν θετικά (παρουσία πλακών βακτηριοφάγων) για τους σωματικούς κολιφάγους, με τον αριθμό των πλακών να κυμαίνεται από 71,4 έως 584800 pfp/100g, 52 δείγματα (37,7%) βρέθηκαν θετικά για τους F-specific RNA βακτηριοφάγους (αριθμός των πλακών από 76,2 έως 17051 p100g) και 33 δείγματα (24%) βρέθηκαν θετικά για τους βακτηριοφάγους του Bacteroides fragilis (αριθμός των πλακών από 194.5 έως 5266,25 pfp/100g). Τόσο για το βακτήριο E. coli όσο και για τους βακτηριοφάγους πραγματοποιήθηκαν διεργαστηριακές αναλύσεις προτύπων, οι οποίες οδήγησαν στο συμπέρασμα ότι οι αντίστοιχες μέθοδοι χαρακτηρίζονται ως αξιόπιστες. Η στατιστική ανάλυση έδειξε ότι το βακτήριο E. coli παρουσιάζει θετική συσχέτιση με τους σωματικούς κολιφάγους, αλλά δεν δείχνει στατιστικά σημαντική συσχέτιση ούτε με τους F-specific RNA βακτηριοφάγους, ούτε με κανέναν από τους ιούς εντερικής προέλευσης. Επίσης, θετική συσχέτιση παρουσίασαν οι αδενοϊοί με τους εντεροϊούς, καθώς και οι σωματικοί κολιφάγοι με τους βακτηριοφάγους του B. fragilis. Η μοναδική συσχέτιση μεταξύ ιών εντερικής προέλευσης και βακτηριοφάγων βρέθηκε για τους αδενοϊούς και τους βακτηριοφάγους του B. fragilis. Εάν αυτό επιβεβαιωθεί σε περαιτέρω μελέτες, τότε η συγκεκριμένη κατηγορία βακτηριοφάγων θα μπορούσε να αποτελέσει έναν καλό δείκτη πρόβλεψης της παρουσίας αδενοϊών σε δείγματα οστρακοειδών. Επιπλέον μελετήθηκε η σχέση που μπορεί να υπάρχει μεταξύ των φυσικοχημικών παραμέτρων και των μικροοργανισμών που εξετάστηκαν. Η επεξεργασία αυτή οδήγησε στο συμπέρασμα ότι το βακτήριο E. coli ανιχνεύεται σε μεγαλύτερα ποσά όταν το διαλυμένο οξυγόνο και η περιεκτικότητα σε άλας του ύδατος είναι αυξημένα. Αντίθετα, αύξηση της θερμοκρασίας οδηγεί σε μείωση της ανίχνευσης του βακτηρίου. Επίσης, η περιεκτικότητα σε άλας φαίνεται να επηρεάζει θετικά και τον ιό της ηπατίτιδας Α, αν και ο μικρός αριθμός θετικών δειγμάτων γι’αυτόν τον ιό δεν μπορεί να επιτρέψει την εξαγωγή ασφαλών συμπερασμάτων. Το pH και το διαλυμένο οξυγόνο των υδάτων οδηγεί σε αύξηση της ανίχνευσης των βακτηριοφάγων του B. fragilis, χωρίς όμως να μπορούμε να ισχυριστούμε ότι κάτι τέτοιο ισχύει, λόγω του μικρού αριθμού θετικών δειγμάτων γι’αυτούς τους βακτηριοφάγους. Τέλος, η αύξηση της θερμοκρασίας των υδάτων φαίνεται να οδηγεί και σε αύξηση της παρουσίας των F-specific RNA βακτηριοφάγων, και το ίδιο παρατηρήθηκε και με την αύξηση του διαλυμένου οξυγόνου στο νερό. Η παρούσα μελέτη αποτελεί την πρώτη διεξοδική έρευνα για την ιογενή κοπρανώδη μόλυνση τον οστρακοειδών στην Ελλάδα. Επιπλέον, αντιπροσωπεύει την πρώτη μελέτη σχετικά με τη αποτελεσματικότητα των οργανισμών - δεικτών ιϊκής μόλυνσης, καθώς και για τη συσχέτιση της μικροβιολογικής επιβάρυνσης των οστρακοειδών με τις φυσικοχημικές παραμέτρους του περιβάλλοντος ύδατος. Η μελέτη κατάλληλων δεικτών που σχετίζονται με την παρουσία εντερικών ιών στα οστρακοειδή οδήγησε σε χρήσιμα συμπεράσματα για τη χρήση της ανίχνευσης των βακτηριοφάγων ως δεικτών ιϊκής μόλυνσης. Εντούτοις, απαιτείται περαιτέρω μελέτη προκειμένου να προσδιοριστεί και η χρήση των βακτηριοφάγων ως δεικτών που θα μαρτυρούν την προέλευση (ανθρώπινη ή ζωική) των εντερικών ιών που ανιχνεύονται στα οστρακοειδή. / In this doctorate investigation, important shellfish growing areas of Greece have been defined and studied. Oysters (Ostrea edulis) and mussels (Mytilus galloprovincialis) were obtained on a monthly basis over an 18 month sampling period. These samples were collected by six (6) different points of Greece and were analyzed for enteroviruses (EV), adenoviruses (Adv), virus of hepatitis A (HAV), Noro viruses I and II (NLVI and NLVII ), bacterium E. coli, as well as for somatic coliphages, F-sperific RNA bacteriophages and bacteriophages of B. fragilis. Moreover, methods were developed for the detection of pathogenic viruses of human origin in the shellfish, as well as for the detection of potential "viral indicators". The methods were examined in order to validate the good quality performance from all the laboratories via interlaboratory analyses. The method that used in this study for the detection of human enteric viruses in the shellfish is based on the export and homogenisation of digestive gland with glycine buffer at pH 10, viral nucleic acid extraction and amplification of the genomes of the analysed human viruses. The procedure applied for detection of E. coli consists on a five tube, three dilution most probable number (MPN) method, while the method for the detection of bacteriophages was the double-agar-layer method. For E. coli analysis, in a total number of 138 samples, 110 samples (79,7%) were found to belong in category A (MPN / 100 g of flesh = < 20 until 220), that it means these samples are characterized as samples of low pollution, 25 samples (18,1%) were found to belong in category B (MPN / 100 g of flesh = 220 until 3500), therefore are characterized as samples of intermediate pollution, inadequate to consumption without precedes process of cleansing, while only 3 samples (2,2%) were found to belong in category C (MPN / 100 g of flesh = 3500 until > 18000), that it means they are samples of high pollution. The viruses that are presented with higher frequency in the shellfish of Eastern Mediterranean are the adenoviruses (34% of samples were found positive for adenoviruses) and follow enteroviruses (16,7% samples they were found positive for enteroviruses). On the contrary, virus of hepatitis A (percentage of positive samples = 4,34%), as well as the Noro I viruses (percentage of positive samples = 2,1%) and Noro II viruses (percentage of positive samples = 1,47%%) are presented in small number of samples. Finally, 80 samples (58%) were found positive (presence of plaques of bacteriophages) for somatic coliphages, with the number of plaques between 71,4 and 584800 pfp / 100 g, 52 samples (37,7%) were found positive for F - specific RNA bacteriophages (number of plaques from 76,2 to 17051 pfp/ 100 g) and 33 samples (24 %) were found positive for the bacteriophages of B. fragilis (number of plaques from 194,5 to 5266,25 pfp / 100 g). Interlaboratory studies involved the testing of reference materials of E. coli and bacteriophages were used as part of the good quality performance assessment program to be applied all over the study, and led to the conclusion that the corresponding methods are characterized by good reliability. According to the statistical analysis of the results, the presence of E. coli seems to be significantly related with the presence of somatic coliphages. However, E. coli do not present significant statistical relation neither with F - specific RNA bacteriophages, nor with all of the viruses of intestinal origin. Also, adenoviruses were significantly related with enteroviruses, as well as somatic coliphages with the bacteriophages of B. fragilis. The unique significant relation between viruses of intestinal origin and bacteriophages was found for the adenoviruses and bacteriophages of B. fragilis. If this is confirmed in further studies, then this category of bacteriophages could constitute a good indicator of forecast of presence adenoviruses in samples of shellfish. Moreover, we studied the relation that can exist between the physic-chemical parameters and the micro-organisms that were examined. This analysis led to the conclusion that E. coli is detected in higher levels when the dissolved oxygen and the salinity of water are increased. On the contrary, increase of temperature leads to reduction of detection of E. coli. Also, the salinity appears to influence positively also virus of hepatitis A, even if the small number of positive samples of this virus cannot allow the export of sure conclusions. The pH and the dissolved oxygen of waters lead to increase of detection of bacteriophages of B. fragilis, but the small number of positive samples for these bacteriophages can’t give safe conclusions. Finally, the increase of temperature of waters appears to lead also to increase of presence of F - specific RNA of bacteriophages, and the same was also observed with the increase of dissolved oxygen in water. This study constitutes the first extensive research for the fecal viral pollution of shellfish in Greece. Moreover, it represents the first study with regard to the effectiveness viral indicators, as well as for the correlation of microbiological parameters of shellfish with the physical-chemical parameters of water. The study of suitable indicators that are related with the presence of enteric viruses in the shellfish led to useful conclusions on the use of detection of bacteriophages as indicators of viral pollution. Nevertheless, further study is required in order to determine also the use of bacteriophages as indicators that will testify the origin (human or animal) of the enteric viruses that are detected in the shellfish.

O presente trabalho tem como objetivo investigar a compreensão dos conceitos de equação e função que serão aplicados na disciplina de Física-Química I do Curso de Química da UFPel. Para isso foram usados como fundamentação teórica o conceito de aprendizagem significativa de Ausubel, Mapas Conceituais e a Epistemologia do “Vê” de Gowin. Também foram utilizados os conceitos matemáticos de equação e função aplicados à Química, assim como a análise e o levantamento de ferramentas computacionais disponíveis na internet e as suas características em termos de aplicabilidade na área do ensino. Para a realização dessa pesquisa se fez uso de pré-testes com os alunos, visando identificar a existência dos subsunçores, assim como, a elaboração de mapas conceituais sobre os conhecimentos matemáticos exigidos na disciplina, particularmente, na Equação dos Gases Ideais. Os resultados foram analisados e permitiram o desenvolvimento de um objeto virtual de aprendizagem que privilegia o uso de equação e função matemática na Química. O objeto virtual de aprendizagem possibilita ao aluno o processo de interação, buscando desenvolver a sua aprendizagem, assim como elementos que promovam a interdisciplinaridade entre a Matemática e a Química. A interdisciplinaridade privilegiará a aplicabilidade de conceitos matemáticos na Equação dos Gases Ideais, especificamente na disciplina de Físico-Química I. / The present work has the objective of investigating the comprehension of the concepts of equation and function that will be applied in the Physics Chemistry subject I from the Chemistry Graduation Course at the UFPel. For that, Ausubel concept of meaningful learning, conceptual maps and the “V” epistemology were used as theoretical background. Besides, the mathematical concepts of equation and function applied to Chemistry were considered and also the analysis and the research of computational tools available on the internet and their characteristics related to the applicability in the teaching area. During the construction period of this research, some pre tests with the students were applied with the purpose of identifying the subsumers as well as the elaboration of the conceptual maps about the mathematical knowledge needed for the subject, mainly for the ideal gas equation. The results were analyzed and they allowed the development of a learning virtual object which privileges the use of mathematical equations and functions inside Chemistry The virtual object allows the student to get into the interaction process searching the development of his learning process as well as elements which promote the interdisciplinarity between Mathematics and Chemistry. Interdisciplinarity will privilege the applicability of mathematical concepts in the Ideal Gas Equation, mainly in the Physics Chemistry Subject I.

O presente trabalho tem como objetivo investigar a compreensão dos conceitos de equação e função que serão aplicados na disciplina de Física-Química I do Curso de Química da UFPel. Para isso foram usados como fundamentação teórica o conceito de aprendizagem significativa de Ausubel, Mapas Conceituais e a Epistemologia do “Vê” de Gowin. Também foram utilizados os conceitos matemáticos de equação e função aplicados à Química, assim como a análise e o levantamento de ferramentas computacionais disponíveis na internet e as suas características em termos de aplicabilidade na área do ensino. Para a realização dessa pesquisa se fez uso de pré-testes com os alunos, visando identificar a existência dos subsunçores, assim como, a elaboração de mapas conceituais sobre os conhecimentos matemáticos exigidos na disciplina, particularmente, na Equação dos Gases Ideais. Os resultados foram analisados e permitiram o desenvolvimento de um objeto virtual de aprendizagem que privilegia o uso de equação e função matemática na Química. O objeto virtual de aprendizagem possibilita ao aluno o processo de interação, buscando desenvolver a sua aprendizagem, assim como elementos que promovam a interdisciplinaridade entre a Matemática e a Química. A interdisciplinaridade privilegiará a aplicabilidade de conceitos matemáticos na Equação dos Gases Ideais, especificamente na disciplina de Físico-Química I. / The present work has the objective of investigating the comprehension of the concepts of equation and function that will be applied in the Physics Chemistry subject I from the Chemistry Graduation Course at the UFPel. For that, Ausubel concept of meaningful learning, conceptual maps and the “V” epistemology were used as theoretical background. Besides, the mathematical concepts of equation and function applied to Chemistry were considered and also the analysis and the research of computational tools available on the internet and their characteristics related to the applicability in the teaching area. During the construction period of this research, some pre tests with the students were applied with the purpose of identifying the subsumers as well as the elaboration of the conceptual maps about the mathematical knowledge needed for the subject, mainly for the ideal gas equation. The results were analyzed and they allowed the development of a learning virtual object which privileges the use of mathematical equations and functions inside Chemistry The virtual object allows the student to get into the interaction process searching the development of his learning process as well as elements which promote the interdisciplinarity between Mathematics and Chemistry. Interdisciplinarity will privilege the applicability of mathematical concepts in the Ideal Gas Equation, mainly in the Physics Chemistry Subject I.

Made available in DSpace on 2016-06-02T19:29:27Z (GMT). No. of bitstreams: 1 3157.pdf: 1685283 bytes, checksum: 5b0ce4290ca01438b5787e5b0a470517 (MD5) Previous issue date: 2010-05-20 / This study aims to characterize the honey production and the local productive honey arrangement, through the evaluation of the land use, honey production, regional flora and product quality. It also aims to stimulate local process of development, rural protagonist and social inclusion of the agricultural workers from the southeast of Mato Grosso. We have evaluated the production of honey during the period of 1987 to 2007, of the towns engaged in APL of apiculture which is available on the SIDRA System by IBGE. The quantification of the use and the topsoil of the soil were obtained through satellite images. We have used SPRING and ArcGIS software for the date using field information and appropriate terminology. We have identified the most important vegetal species for the production of honey. We have determined ten physic-chemistry parameters and we have evaluated the characteristics of the labeling of the commercialized honey. The town of Cáceres (42%) presents the biggest honey production fallowed by the towns of Comodoro (11%), then Poconé (7%), then Reserva do Cabaçal (7%), then Conquista do Oeste (6%), and Porto Espiridião (6%). The production of honey is not the principal economic activity of the apiculture of this region. Most of them have just one apiary with a small number of beehives and they lack time of experience on this activity. The extraction and benefit of honey is done in manufactured fashion and the commercialization of their honey is done directly to the consumers, in the local retailer market. The level of technology implemented in this activity is low and the farmers have also a low level of professionalization. Natural Vegetation was the predominant class of the use of the soil on the majority of the apiaries. The kinds of vegetal covering were summarized as it follows: Savannas; Ecological Tension; Seasonal Forest; Riparian Formation. Grazing was the only kind of entropic agriculture use. Not Agriculture Entropic Areas were less representative. The classes of the use of soil and the different kinds of vegetal covering printed differences in the richness of plants and in the production of honey of the apiaries. The evaluation of the use of the soil and the vegetal covering of it around the apiaries allows the establishment of the appropriate management and the ways of sustainable uses of the natural resources. The results of physic-chemistry analysis for the samples of honey showed that the median for humidity, redactor and non redactor sugars were among the required patterns by the MAPA. The percentage of insolates solids and the fixed mineral residuals remained over. The Fiehe reaction indicated the presence HMF in others samples. All of the brands evaluated presented some parameters over the maximum limit allowed by the Brazilian legislation, pointing out production problems, as well as collecting and processing of honey. Only two kinds of honey available in the local market, from Cáceres and some other regions, have the sanitary inspection validity. The labeling of the majority of the trades are not in conformity with nowadays legislation, and it ignores the important instrument of identification of origins of honey, which is a guarantee of products with quality and security to the customer. The apiaries represent a way of sustainable use of preserved natural vegetation areas, degradation grazing areas and existing capoeiras, besides, this activity shows itself compatible with some others activities already consolidated in the region of southeast of Mato Grosso. / O estudo teve o objetivo de caracterizar a produção apícola e o arranjo produtivo local de apicultura, por meio da avaliação dos usos da terra, produção de mel, flora regional e qualidade do produto, visando estimular processos locais de desenvolvimento, protagonismo rural e inclusão social de agricultores da região Sudoeste de Mato Grosso. Foi avaliada a produção de mel no período de 1987 a 2007, dos municípios participantes do APL de Apicultura, disponíveis no Sistema SIDRA do IBGE. A quantificação do uso e cobertura da terra foi obtida por imagens de satélite, tratadas nos software SPRING e ArcGIS, utilizando informações de campo e terminologia apropriada. Foram identificadas as espécies vegetais, importantes para a produção do mel. Foram determinados dez parâmetros físico-químicos do mel e avaliado características de rotulagem do mel comercializado. O município de Cáceres (42%) é o maior produtor de mel, seguido por Comodoro (11%), Poconé (7%), Reserva do Cabaçal (7%), Conquista do Oeste (6%) e Porto Esperidião (6%). A apicultura não é a principal atividade econômica dos apicultores da região, a maioria deles tem apenas um apiário, com pequeno número de colméias e possui pouco tempo de experiência na atividade. A extração e beneficiamento do mel são realizados de forma artesanal e a comercialização é feita de forma direta ao consumidor, no mercado varejista local. O nível tecnológico empregado na atividade é baixo e o apicultor tem pouco grau de profissionalização na atividade. A Vegetação Natural foi a classe predominante de uso da terra na maioria dos apiários. Os tipos de cobertura vegetal foram reunidos em: Savanas, Tensão Ecológica, Floresta Estacional e Formações Ripárias. Pastagem foi a única forma de uso Antrópico Agrícola. Áreas Antrópicas Não Agrícola foram pouco representativas. As classes de uso da terra e os tipos de cobertura vegetal do entorno dos apiários imprimiram diferenças na riqueza de plantas e na produção de mel dos apiários. A avaliação do uso e cobertura da terra permite o planejamento e a gestão da apicultura, possibilitando estabelecer manejo apropriado e formas de utilização sustentável dos recursos naturais. Os resultados da análise físico-química das amostras de mel mostraram que as médias para umidade, açúcares redutores e não redutores ficaram dentro dos padrões estabelecidos pelo MAPA. Os percentuais de sólidos insolúveis e resíduos minerais fixos ficaram acima. A reação de Fiehe indicou a presença de HMF em outras amostras. Todas as marcas avaliadas apresentaram algum parâmetro acima do limite máximo permitido pela legislação brasileira, evidenciando problemas na produção, coleta ou processamento do mel. Somente duas marcas de mel disponíveis no mercado local, oriundas de Cáceres e outras regiões, possuem selo de inspeção sanitária. O rótulo da maioria das marcas não está em conformidade à legislação vigente e ignora importante instrumento de identificação da origem do mel, garantia de produtos com qualidade e segurança ao consumidor. A apicultura representa uma forma de uso sustentável de áreas de vegetação natural preservadas, áreas de pastagens degradadas e capoeiras existentes, além disso, a atividade mostra-se ainda compatível com outras atividades já consolidadas na região Sudoeste de Mato Grosso.