OBJECTIVE: Prior studies suggested rapid weight gain in infancy as a risk factor for the development of obesity. Our aim was to determine if early-childhood treatment for malnutrition is associated with the development of obesity in later-childhood. METHODS: This was a retrospective chart review of 194 children who had been treated and discharged from the Growth and Nutrition Program (GNP) from 1/1/2000 to 7/30/2014, with at least one height and weight recorded after discharge. Subjects predisposed to obesity due to medical conditions or medications were excluded. Obesity was defined using WHO and CDC growth charts, body mass index ≥95th percentile for age and sex, and compared to published prevalence rates. Potential predictors of obesity prevalence were also examined. RESULTS: None of the 194 subjects were obese at time of discharge from GNP. Over the 20-year follow-up period, 7% became obese (well below the national obesity prevalence of 18.5%1. 3 of 11 (27.3%) patients prescribed preterm infant formula became obese in contrast to 10 of 173 (5.8%) who were not prescribed (p=0.007). 6 of 27 (22.2%) subjects identified as African American became obese in contrast to 7 of 157 (4.5%) who did not identify (p=0.001). CONCLUSION: While overall prevalence of obesity was lower than that of the general population, children requiring preterm infant formula and/or identified as African American were more likely to develop obesity in childhood. Findings support the need for more anticipatory guidance regarding preterm infant formula and aggressive weight management and planning prior to GNP discharge. / 2022-06-07T00:00:00Z

京都大学 / 新制・課程博士 / 博士(医学) / 甲第24488号 / 医博第4930号 / 新制||医||1063(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 花川 隆, 教授 渡邉 大, 教授 村井 俊哉 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

Background: Recent research is revealing the high rate of false-positive screening results for IEMs in the NICU population. No study published to date has specifically studied the possible relationship between nutrition and newborn screening in this population. Objective: It is suspected that NICU infants who receive PN are more likely to have abnormal newborn screening results than infants who receive EN. An understanding of the role of nutrition will assist in developing protocols for screening in the NICU and decrease false-positives. Design: Infants admitted to the NICU between January 1-June 30, 2009 were included in this retrospective chart review study (n=339). The type of nutrition and timing of its initiation was recorded and compared to newborn screening results to identify correlations with false-positives. Statistical analysis included means, percentages, Fisher's exact test, Chi-square test, and the Cochran-Mantel-Haenszel test. Results: Nutrition type was significantly associated with newborn screening (p<0.001); those who received parenteral nutrition were more likely to have a false-positive. For infants who also received PN, EN of breast milk exclusively increased risk of an abnormal screen more than formula exclusively or breast milk plus formula. The timing of parenteral nutrition had no effect on screening. Premature infants who received PN exclusively had a higher percentage of false-positives than those who received EN Conclusions: Although the hypothesis could not be statistically supported, PN appears to contribute to false-positive newborn screens. More research is needed to ascertain the role of EN and GA in newborn screening and to develop standardized protocols. / Master of Science

The present doctoral thesis focuses mainly on the experiences of 20 women and men of becoming and being parents of a prematurely born child and on their perceptions of their contacts with health and medical care services. An additional aim was to investigate the utilisation of health care during the first year of life and its relation to high-risk diagnoses in the neonatal period. Data were obtained by interviews of the 20 sets of parents to infants born at a gestational age of &lt;34 weeks with no serious congenital defects. These parents were interviewed 1-2 weeks after the infant’s birth and at 2, 6 and 18 months of age. The interview texts were subjected to phenomenological and content analysis. Data was also obtained from the records of 36 infants born at a gestational age of &lt;31 weeks and with a birth weight of &lt;1500 g and 36 full-term infants. The records included information regarding contact with health care services including the child health centres (CHCs) and the outpatient clinics of the paediatric and ophthalmic clinics. Finally data from the other two quantitative studies were obtained from three questionnaires: Quality of Care from The Patient’s Perspective, The Swedish Parenthood Stress Questionnaire and The Toddler Behaviour Questionnaire. The result of the studies showed that the utilisation of health care by the very low birth weight infants was higher than that by the fullterm infants in paediatric and ophthalmic outpatient clinics. High-risk diagnoses in the neonatal period did not correlate with utilisation of care except for visits to the paediatric outpatient clinic, especially planned visits. The internalisation of parenthood was described by the parents as a time-dependent process, with four syntheses of experiences – alienation, responsibility, confidence and familiarity. The development and construction of the mothers’ and fathers’ parental identity followed a pattern that could be summarised into three themes: Unexpected start of parenthood, integration of parenthood into the sense of identity and recognition of parental identity. Important turning points in experiences of parenthood often occurred when the infant could be removed from the incubator, when it was discharged from the ward, and when the infant appeared normal compared to full-term infants. The quality of care was judged, from the parents’ and nurses’ perspective. In general the subjective importance of the given care was rated higher than the care actual given in both neonatal care and care at the CHCs. Higher ratings were given to neonatal care compared with the care at CHSs for medico-technical competence. It was also of importance, of both parents, to receive an optimal identity-oriented approach and socio-cultural atmosphere in the neonatal care and the care at the CHSs. High-risk diagnoses in the newborn did not affect the answers. Parental stress of very preterm children, at 18 months of age, did not differ notably from those parents of children born somewhat less premature, but they assessed their children as being rather later in the development of social behaviours. Parents of children who had had a difficult neonatal period were not more stressed when the child was 18 months old than those who had no problems in the neonatal period. In sum, this research project showed that the parents’ expectations and experiences of becoming mothers and fathers in preterm birth was a process of integrating the unexpected start of parenthood into the parents’ sense of identity and their way of being. When the parents developed a relationship with the infant, actively participated in its care on the basis of their own preferences, and received recognition as parents, this process was strengthened. It is therefore important that the professional caring of the staff should meet and involve the natural caring of the parents.

Oral feeding competency is a primary requirement for preterm infant hospital release. Currently there is no widely accepted method to objectively measure oral feeding. Feeding consists primarily of the integration of three individual feeding events: sucking, breathing, and swallowing, and the objective of feeding coordination is to minimize aspiration. The purpose of this work was to quantify the infant feeding process from signals obtained during bottle feeding and ultimately develop a measure of feeding coordination. Sucking was measured using a pressure transducer embedded within a modified silicone bottle block. Breathing was measured using a thermistor embedded within nasal cannula, and swallowing was measured through the use of several different piezoelectric sensors. In addition to feeding signals, electrocardiogram (ECG) signals were obtained as an indicator of overall infant behavioral state during feeding. Event detection algorithms for the individual feeding signals were developed and validated, then used for the development of a measurement of feeding coordination. The final suck event detection algorithm was the result of an iterative process that depended on the validity of the signal model. As the model adapted to better represent the data, the accuracy and specificity of the algorithm improved. For the breath signal, however, the primary barrier to effective event detection was significant baseline drift. The frequency components of the baseline drift overlapped significantly with the breath event frequency components, so a time domain solution was developed. Several methods were tested, and it was found that the acceleration vector of the signal provided the most robust representation of the underlying breath signal while minimizing baseline drift. Swallow signal event detection was not possible due to a lack of available data resulting from problems with the consistency of the obtained signal. A robust method was developed for the batch processing of heart rate variability analysis. Finally a method of coordination analysis was developed based on the event detection algorithm outputs. Coordination was measured by determining the percentage of feeding time that consisted of overlapping suck and breath activity.

Bakgrund: Varje år behöver mer än 10 000 nyfödda barn i Sverige vård på en neonatalvårdsavdelning. Vårdtiden präglas av åtskilliga blodprovstagningar som orsakar barnet smärta och ökat vårdlidande. Sjuksköterskans ansvar innefattar att agera för barnets bästa i alla situationer, men det kan saknas tydliga riktlinjer för hur detta ska ske på arbetsplatsen. Syfte: Syftet med studien är att beskriva sjuksköterskors resonemang och arbetssätt vid blodprovstagning på barn på neonatalvårdsavdelning, samt att undersöka hur barnets bästa finns i fokus vid proceduren. Metod: Studien utformades med en kvalitativ studiedesign i avsikt att generera kunskap om sjuksköterskornas erfarenheter vid blodprovstagning. Tolv intervjuer genomfördes utifrån en semistrukturerad intervjuguide med sjuksköterskor verksamma vid en neonatalvårdsavdelning på ett svenskt universitetssjukhus. Den insamlade datan analyserades genom en induktiv innehållsanalys. Resultat: Vilken stickmetod sjuksköterskorna använde var anpassat efter barnet och situationen. De såg till barnets förutsättningar och planerade provtagningen efter vad barnet klarade av, i samråd med föräldrarna. Sjuksköterskorna strävade efter föräldranärvaro vid provtagningen och åtog adekvata smärtlindringsmetoder i den mån som situationen tillät. Slutsats: Sjuksköterskorna hade genomgående barnets bästa i fokus vid provtagningsproceduren. De såg en komplexitet i sin roll att både vara den som orsakar barnet smärta, men även vara den som ska minska barnets vårdlidande. / Background: Every year, more than 10 000 newborn children require hospitalization in a neonatal intensive care unit. During this time, they are subjected to several painful blood sampling procedures that will cause them suffering to some extent. The nurses’ responsibility is to act on what is best for each child at all times but clearer guidelines for how that should be executed in practical nursing care might be needed. Aim: To describe the reasoning and the working manner of the nurses when performing blood sampling on children. The study also aims to examine how the child’s best interest is taken into account before and during this procedure. Method: A qualitative design was used for this study. Semi-structured interviews were conducted with 12 registered nurses who work in the neonatal intensive care unit at a Swedish University Hospital. Collected data were analysed using inductive content analysis. Results: Regardless of which technique the nurses chose when performing blood sampling it was assessed as the most appropriate one for each child. They took the children’s prerequisites and ability to cope into account and planned the procedure in consensus with their parents. Nurses strove to encourage parents to stay close to and support their children when blood sampling was conducted, and adequate analgesic actions were undertaken. Conclusion: Nurses had the children’s best at heart all through the process of blood sampling. Their role as both inflicting and relieving pain was considered to be complex in the aim to avoid suffering.

Preterm infants have multiple health complications due to their underdeveloped neurological systems. Bottle-feeding difficulties are one complication that leads to pulmonary illness secondary to aspiration. Preterm infants exhibit clinical markers when experiencing distress during bottle-feeding. Training caregivers to identify clinical markers reduces the risk for aspiration. Simulation training provides a safe learning environment without harming patients. Twenty-two speech-language pathology and pre-requisite students divided into two simulation groups, video-simulation (N=12) and in-vivo simulation (N=10), were trained to document clinical markers of distress exhibited by preterm infants and make clinical judgments about bottle-feeding. Students rated their levels of anxiety during simulation training. Results revealed that students trained using video-simulation performed with higher clinical judgment scores and lower anxiety levels than students who received in-vivo training. Students’ knowledge of and ability to identify distress markers in preterm infants during bottle-feeding significantly improved after training in both groups without group differences.

Background: Physiological studies have shown that many preterm infants and infants with chronic lung disease may suffer chronic hypoxaemia, which possibly leads to poor growth and development. Anecdotal reports indicate that there is a drive to increase the oxygen saturation target range to a higher level in these infants due primarily to perceived benefits derived from clinical experience and from uncontrolled observational studies of babies discharged on home oxygen. Objective The BOOST (Benefits Of Oxygen Saturation Targeting) trial is the first randomised trial to assess the long-term benefits and harms of two different oxygen saturation target ranges. Methods: BOOST was a multicentre, double blinded, randomised controlled trial that enrolled 358 infants born at less than 30 weeks� gestation who remained oxygen-dependent at 32 weeks postmenstrual age. They were randomly assigned to target either a functional oxygen saturation range of 91-94% (standard or control group) or 95-98% (higher or treatment group). The primary outcomes were growth and neurodevelopmental measures at 12 months corrected age. Secondary outcomes included length of hospital stay, retinopathy of prematurity, health service utilisation, parental stress, and infant temperament. Results: Prognostic baseline characteristics did not differ between the two groups. Mean birth weight and gestational age of enrolled infants was 917g and 26.5 weeks respectively. The rate of antenatal corticosteroid use was 83%.

<p>Aim: To examine the reaction of extreme preterm infants with regarding to body temperature and transepidermal water loss during skin-to-skin care according to Kangaroo Mother Care (KMC) during the infant’s first week of life. Method: This was a descriptive quantitatively designed study, which was a pilot study within the framework of a larger project. Nine children, with a median gestational age of 24.91 weeks, were examined by measuring body temperature (axilla and skin temperature) as well as transepidermal water loss before, during and after KMC. Results: The study showed that skin temperature tended to rise during KMC, especially for those children who were nursed with KMC for more than 60 minutes. Eight out of nine children had, after completing KMC, a normal axilla temperature. As was expected, transepidermal water loss was elevated when measured both pre and post test. Despite this, the children showed normal body temperature. Conclusion: The results of the study support that KMC can create a micro-climate that, for at least one hour, makes it possible for even extreme preterm infants to maintain a body temperature within the normal range during their first week of life.</p>

<p>While constituting a well functioning interface with the aqueous environment in utero, the skin offers a poor barrier after very preterm birth. As a result, transepidermal water loss (TEWL) is high, a fact which has important clinical consequences in these infants. To investigate the transport of water through perinatal skin and the potential role of aquaporin (AQP), a water channel protein, in this process, we determined TEWL in a group of extremely preterm infants, and in an experimental rat model we analyzed the expression and distribution of AQP in perinatal skin in relation to TEWL, skin surface hydration and water content. The effects of antenatal corticosteroids (ANS) and of restricted intake of fluids and nutrients on barrier characteristics of the perinatal skin and its AQP expression were also studied.</p><p>In infants born at 24 and 25 weeks of gestation TEWL was very high in the first days after birth and decreased with increasing postnatal age. At a postnatal age of 4 weeks, TEWL was still twice as high as previously reported in infants born at a gestational age of 25-27 weeks and four times higher than in infants born at term. In the rat model, immunohistochemical analysis revealed that AQP1 and AQP3 are abundantly expressed in the skin. AQP1 was expressed exclusively in dermal capillaries and AQP3 in basal layers of the epidermis. AQP1 and AQP3 mRNA as assessed by semiquantitative RT-PCR was higher in fetal than in adult skin. As in infants, TEWL and skin surface hydration were inversely related to gestational age in the rat. In preterm rat pups exposed to ANS, TEWL and skin surface hydration were lower than in unexposed controls, and AQP3 expression was selectively induced by ANS. In term newborn rat pups, restriction of fluid and nutrient intake resulted in a higher skin water content and higher TEWL early after birth, while at an age of 7 days TEWL was lower in fasting rat pups than in controls, although skin water content was still higher.</p><p>To conclude, TEWL is very high in extremely preterm infants early after birth and then decreases at a slower rate than previously reported for a group of slightly more mature infants. </p><p>This is the first time that the distribution and gene expression of AQP1 and AQP3 have been demonstrated in perinatal skin. The localization and expression of AQP in the skin might indicate that these water channels are involved in the regulation of skin hydration and transepidermal water transport in the fetus and newborn infant.</p>