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Made available in DSpace on 2015-04-14T13:35:17Z (GMT). No. of bitstreams: 1 433931.pdf: 2326091 bytes, checksum: 440c15a6896a635a762bbff20a3d5cf0 (MD5) Previous issue date: 2011-08-29 / OBJETIVO : O objetivo deste estudo ? verificar o tempo m?nimo de ades?o do polietileno poroso no estroma corneano de porcos como alternativa para por??o h?ptica de pr?tese de c?rnea. M?TODOS : Foi realizado estudo de interven??o animal com implante de cinco discos de polietileno poroso em estroma corneano de cinco olhos de diferentes porcos com an?lise de ades?o em tempos arbitrados em 30/45/60/75 e 90 dias de exposi??o. Foram analisados as ?reas e dimens?es fractais das imagens de Microscocopia Eletr?nica de Varredura de uma das extremidades da sec??o central dos cinco discos e comparados entre si. RESULTADOS : A rela??o da ?rea da sec??o da periferia do conjunto estroma/polietileno foi de 53835/29374 na an?lise de 30 dias (P1OC); 53585/24758 em 45 dias (P2OC); 115119/117455 em 60 dias (P3OC); 94771/91579 em 75 dias (P4OC) e 85860/81742 em 90 dias (P5OC), p= 0,041.A rela??o da Dimens?o Fractal entre o conjunto estroma/polietileno foi de 1,0534/1,1397 em 30 dias (P1OC); 1,0544/1,1127 em 45 dias (P2OC); 1,0370/1,0410 em 60 dias (P3OC); 1,0471/1,1016 em 75 dias (P4OC) e 1,066/1,1154 em 90 dias (P5OC), p= 0,019. Sendo a menor diferen?a de Dimens?o Fractal 0,004 aos 60 dias e menor diferen?a percentual de ?rea de 3,36 aos 75 dias. CONCLUS?O : A an?lise das imagens demonstraram melhor ades?o entre o estroma e o polietileno a partir do 60? dia de exposi??o, apesar do n?mero pequeno da amostra. N?o foi evidenciado extrus?o em nenhum dos per?odos analisados. Pode se afirmar que o polietileno poroso se manteve biocompat?vel no tempo observado, no entanto sendo necess?rio futuro estudo para verificar seu comportamento a longo prazo

Efficient and accurate repair of DNA double strand breaks (DSBs) is required to maintain genomic stability in both eukaryotes and prokaryotes. In Escherichia coli, DSBs are repaired by homologous recombination (HR). During this process, DNA synthesis needs to be primed and templated from an intact homologous sequence to restore any information that may have been lost on the broken DNA molecule. Two critical late stages of the pathway are repair DNA synthesis and the processing of Holliday junctions (HJs). However, our knowledge of the detailed mechanisms of these steps is still limited. Our laboratory has developed a system that permits the induction of a site-specific DSB in the bacterial chromosome. This break forms in a replication dependent manner on one of the sister chromosomes, leaving the second sister chromosome intact for repair by HR. Unlike previously available systems, the repairable nature of these breaks has made it possible to physically investigate the different stages of DNA double-strand break repair (DSBR) in a chromosomal context. In this thesis, I have addressed some fundamental questions relating to repair DNA synthesis and processing of HJs by using a combination of mutants defective in specific biochemical reactions and an assay that I have developed to detect repair DNA synthesis, using a polar termination sequence (terB). First, by using terB sites located at different locations around the break point, it was shown that the DnaB-dependent repair forks are established in a coordinated manner, meaning that the collision of the repair forks occurs between two repair DNA synthesis initiation sites. Second, DSBR was shown to require the PriB protein known to transduce the DNA synthesis initiation signal from PriA protein to DnaT. Conversely, the PriC protein (known as an alternative to PriB in some reactions) was not required in this process. PriB was also shown to be required to establish DnaB-dependent repair synthesis using the terB assay. Third, the establishment and termination of repair DNA synthesis by collision of converging repair forks were shown to occur independently of HJ resolution. This conclusion results from the comparison of the viability of single and double mutants, deficient in either the establishment of DNA synthesis, HJ resolution or in both reactions, subjected to DSBs and from the study of the DNA intermediates that accumulated in these mutants as detected by two-dimensional gel electrophoresis. Fourth, the role of RecG protein during DSB repair was investigated. Solexa sequencing analyses showed that recG null mutant cells undergoing DSBs accumulate more DNA around the break point (Mawer and Leach, unpublished data). This phenomenon was further investigated by two different approaches. Using terB sites in different locations around the break point and ChIP-Seq analyses to investigate the distribution of RecA in a recG null mutant demonstrating that the establishment of repair forks depends on the presence of RecG. Further studies using PriA helicase-dead mutant showed that the interplay between RecG and PriA proteins is essential for the establishment of correctly oriented repair forks during DSBR. As a whole, this work provides evidence on the coordinated nature of the establishment and termination of DNA synthesis during DSBR and how this requires a correct interplay between PriA-PriB and RecG. A new adapted model of homologous recombination is presented.

?rea de concentra??o: Educa??o e Tecnologias aplicadas em Institui??es Educacionais. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-07-25T17:09:12Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fabiano_kenji_aoki.pdf: 1033535 bytes, checksum: 2f0dc1bd0aca41817d8063177f6e1124 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-10-04T19:44:59Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fabiano_kenji_aoki.pdf: 1033535 bytes, checksum: 2f0dc1bd0aca41817d8063177f6e1124 (MD5) / Made available in DSpace on 2018-10-04T19:44:59Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fabiano_kenji_aoki.pdf: 1033535 bytes, checksum: 2f0dc1bd0aca41817d8063177f6e1124 (MD5) Previous issue date: 2017 / O tema da avalia??o de institui??es educacionais ? bastante complexo. Nasce do anseio natural de uma organiza??o em conhecer a si pr?pria e de exig?ncias legais e normativas de avalia??o atualmente existentes para todos os n?veis da educa??o, da b?sica ? superior. Passa pelos esfor?os de organiza??o, manuten??o e produ??o de informa??es que, ao mesmo tempo em que atendem a norma legal, transformam-se em valiosa ferramenta para a tomada de decis?es, quando sensibilizada a c?pula hier?rquica de uma institui??o sobre a import?ncia do processo de autoavalia??o institucional. O Sistema Nacional de Avalia??o da Educa??o Superior (SINAES), em vigor desde 2004, examina as institui??es de ensino superior (IES), os cursos de gradua??o e o desempenho dos estudantes. O presente trabalho teve por objetivo a elabora??o do manual de processo de autoavalia??o institucional com vistas a apoiar os trabalhos conduzidos pela Comiss?o Pr?pria de Avalia??o (CPA) das IES. A utiliza??o de manuais em ambientes corporativos contribui para a compreens?o e a consolida??o de conhecimentos inerentes ? ?rea de atua??o do profissional e para o norteamento das a??es de grupos de trabalho orientados para um mesmo objetivo. A metodologia utilizada envolveu pesquisas bibliogr?fica e documental, tendo em vista o estudo explorat?rio de enfoque qualitativo. / Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Educa??o, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The subject of evaluation of educational institutions is quite complex. It is born of the natural yearning of an organization to know itself and the legal requirements and evaluation regulations that currently exist for all levels of education, from basic to higher education. It goes through the efforts of organization, maintenance and production of information that, while complying with the legal norm, become a valuable tool for decision making, when sensitized the hierarchical leadership of an institution on the importance of the process of self-evaluation institution. The National System for the Evaluation of Higher Education (SINAES), in force since 2004, examines higher education institutions (HEIs), undergraduate courses and student performance. The objective of the present work was to elaborate a process manual of the institutional self-evaluation to support the work carried out by the Self-Evaluation Committee (CPA) of HEI. The use of manuals in corporate environments contributes to the understanding and consolidation of knowledge inherent in the professional's area of activity and to the orientation of work group actions oriented toward the same objective. The methodology used involved bibliographical and documentary research, in view of the exploratory study of a qualitative approach.

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Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-05-18T17:34:10Z No. of bitstreams: 1 DIS_ANTONIO_GOYA_DE_ALMEIDA_MARTINS-COSTA_PARCIAL.pdf: 608697 bytes, checksum: 8c4b8e7aedd70bed44e62abaf5a3966f (MD5) / Made available in DSpace on 2017-05-18T17:34:10Z (GMT). No. of bitstreams: 1 DIS_ANTONIO_GOYA_DE_ALMEIDA_MARTINS-COSTA_PARCIAL.pdf: 608697 bytes, checksum: 8c4b8e7aedd70bed44e62abaf5a3966f (MD5) Previous issue date: 2016-12-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This dissertation aim to study a set of problems that derived from the equiparation of the omission to the action in improper omissive crimes (Gleichstellungsproblematik) in contemporary criminal law, all that from the theory of the position of guarantee (Theorie der Garantenstellung). In other words, this research intends to investigate the merits and demerits of some of the most important theories found in contemporary literature commonly used to explain the aforementioned phenomenon. The methodology select by us to solve the questions raised was the bibliographical review of several studies presented in order to elucidate the problem at hand. The dissertation is divided into five chapters. The first one seeks to demonstrates how this problem was posed by the criminal dogmatics and, consequently, aim to discusses the reasons why a set of notorious proposals defend that the two forms of conduct (omission of the impediment of a result and its production by an action) must receive the same treatment under criminal law. The next three chapters analyze three different theories built around the problem we seek to investigate (material-formal theories, Jakobs theory and Sch?nemann's theory). In each chapter, after characterizing the aforementioned theories, we intend to list the objections commonly made by de doctrine to them and, afterwards, present a set of critical observations. The fifth chapter summarizes the main points discussed in the course of this investigation, concluding that the Sch?nemann's approach is the theoretical perspective that - if nuanced with a formal-material theory - offers us a more plausible and coherent solution for the problems posed by the theory of the position of guarantee. This positioning surely reduces the scope of guarantee of supervision and control of sources of danger only to obligations of insurance in legal traffic (Verkehrssicherungspflichten), denying rescue duties (Rettungspflichten) in the position of guarantee by interference. One of the main conclusions of the research is that the starting point for the solution of the ?problem of equalization? must be an argument based on the idea of similarity. That similarity must be found in the analytical structure that justifies the imputation of the result to both the commission and the omission conducts. / A disserta??o tem como objeto de estudo a problem?tica da equipara??o da omiss?o ? a??o nos crimes omissivos impr?prios (Gleichstellungsproblematik) no direito penal contempor?neo a partir da teoria da posi??o de garantia (Theorie der Garantenstellung). O objetivo ? investigar o fundamento da posi??o de garantia analisando os m?ritos e dem?ritos de algumas das teorias encontradas na literatura contempor?nea para explicar esse fen?meno. A metodologia empregada foi a revis?o bibliogr?fica, com exposi??o e an?lise de diversas propostas de solu??o da problem?tica da equipara??o. A disserta??o est? dividida em cinco cap?tulos. O primeiro deles demonstra a forma como o referido problema se colocou para a dogm?tica penal e discorre sobre as diversas propostas que a doutrina apresentou para tentar justificar que duas formas diversas de conduta (omiss?o do impedimento de um resultado e a sua produ??o por uma a??o) recebessem o mesmo tratamento pelo direito penal. Os tr?s cap?tulos seguintes analisam tr?s propostas completamente diferentes de fundamenta??o te?rica da posi??o de garantia que est?o presentes no direito penal contempor?neo (teorias materiaisformais, teoria de Jakobs e teoria de Sch?nemann). Em cada um desses cap?tulos, ap?s a exposi??o da teoria, apresentam-se as obje??es feitas a ela que foram encontradas na literatura, tomando-se posi??o. O quinto cap?tulo faz uma recapitula??o e s?ntese dos principais pontos tratados ao longo da investiga??o, chegando-se ? conclus?o de que a teoria de Sch?nemann, matizada com uma teoria material-formal ? aquela que oferece uma solu??o mais plaus?vel e coerente para a teoria da posi??o de garantia. Esse posicionamento acarreta a redu??o do ?mbito da garantia de supervis?o e controle de fontes de perigo apenas a deveres de asseguramento no tr?fego jur?dico (Verkehrssicherungspflichten), negando os deveres de salvamento (Rettungspflichten) na posi??o de garantia por inger?ncia. Uma das principais conclus?es ? que o ponto de partida para a resolu??o da problem?tica da equipara??o deve ser um argumento de similitude, o qual h? de ser encontrado na estrutura que justifica a imputa??o do resultado tanto ? comiss?o quanto ? omiss?o.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-09-05T19:49:55Z No. of bitstreams: 1 SuzanaCarolinaDosSantosDutraDeMacedoCosta_DISSERT.pdf: 1466627 bytes, checksum: 3383ee54ee3ebe231c9656df019a5c91 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-09-15T23:14:17Z (GMT) No. of bitstreams: 1 SuzanaCarolinaDosSantosDutraDeMacedoCosta_DISSERT.pdf: 1466627 bytes, checksum: 3383ee54ee3ebe231c9656df019a5c91 (MD5) / Made available in DSpace on 2017-09-15T23:14:17Z (GMT). No. of bitstreams: 1 SuzanaCarolinaDosSantosDutraDeMacedoCosta_DISSERT.pdf: 1466627 bytes, checksum: 3383ee54ee3ebe231c9656df019a5c91 (MD5) Previous issue date: 2017-04-26 / A moradia ? um direito social garantido pela Constitui??o Brasileira de 1988; sua concretiza??o, de acordo com tratados internacionais ratificados pelo Estado Brasileiro, imp?e a observ?ncia de v?rios outros direitos, como, por exemplo, o direito a um n?vel de vida adequado com igualdade de acesso de todos aos bens p?blicos e servi?os de qualidade. Ao mesmo tempo em que figura como um direito social, a moradia, enquanto bem f?sico, se insere no contexto de mudan?as econ?micas, pol?ticas e territoriais que ocorreram, mundialmente, sob a ?gide da hegemonia do pensamento e das pr?ticas neoliberais, tornando-se, cada vez mais, um elemento de consumo do mercado. A invers?o do sentido da produ??o social da moradia ? de direito para mercadoria ? condiciona sua concretiza??o a um prop?sito quantitativo, uma meta, substituindo a luta pela moradia pela luta ao cr?dito. Em face dessa din?mica, a pesquisa partiu da premissa de que a converg?ncia da Moradia como mais um direito do Consumidor- Cidad?o, em vez de um Direito Social, ? provocado, tamb?m, pela ?nfase que as pol?ticas p?blicas assumiriam, mais recentemente, em corresponder aos interesses de mercado, isto ?, proporcionar uma acumula??o do capital por meio da amplia??o da mercadoria ?moradia social? como objeto de consumo. Tomando como objeto a rela??o entre Moradia e Cidadania, a pesquisa teve como objetivo compreender os efeitos da pol?tica de moradia na consolida??o da Cidadania como elemento de integra??o ao Direito ? Cidade. Os questionamentos que est?o na base da pesquisa s?o: Pode uma pol?tica habitacional ampliar ou prejudicar o sentido de Cidadania de parte da popula??o? ? a moradia condi??o b?sica para a Cidadania ocorrer de modo ampliado? Os resultados do estudo revelaram que a aquisi??o da casa pr?pria por um programa do Governo gera no indiv?duo a sensa??o de pertencimento social, concedendo-lhe, implicitamente, o status de ?cidad?o?, na medida em que ao sair de condi??es ilegais e/ou irregulares de moradia e assumir deveres advindos de uma moradia legal e regular o indiv?duo passa a se sentir portador de direitos, com voz para reivindic?-los perante o Estado, no entanto essa n?o ? a cidadania plena. A condu??o da investiga??o do objeto de pesquisa foi orientada por uma compreens?o dial?tica da discuss?o sobre Cidadania e Moradia, que permitiu, com base na revis?o bibliogr?fica e em pesquisa de campo realizada em empreendimento da faixa 1, do Programa Minha Casa Minha Vida, captar a ess?ncia dessa rela??o que pretendemos estabelecer. / Housing is a social right guaranteed by the 1988 Brazilian Constitution; its implementation imposes the observance of several other rights according to international treaties ratified by the Brazilian State, such as the right to an adequate standard of living with equal access to all public goods and quality services. Housing is at the same time a social right and a physical good. It is inserted in the context of economic, political and territorial changes that occurred worldwide under the aegis of neoliberal thought and practices, becoming yet another element of consumption in the market. The inversion of the direction in the social production of housing - from a right to goods - conditions its realization to a quantitative purpose, a goal, replacing the struggle for housing with the fight against credit. Given this new dynamic, the research started from the premise that the convergence of Housing as another Consumer-Citizen right, rather than a Social Law, is also caused by the emphasis that public policies have more recently taken in meeting the interests of the market, that is, to provide an accumulation of capital through the expansion of the commodity "social housing" as an object of consumption. The research has as its subject the relationship between Housing and Citizenship and it aims to understand the effects of housing policy on the consolidation of Citizenship as an element of integration regarding to the Right to the City. The questions which are the basis of the research are: Can a housing policy increase or impair the sense of citizenship from a part of the population? Is housing the basic condition for Citizenship to occur in an expanded way? The results of the study revealed that the acquisition of an own property through a government program generates a sense of social belonging to the individual, implicitly granting him/her the status of 'citizen'. When leaving illegal conditions and/or irregular housing and assuming duties resulting from a legal and regular housing the individual becomes a holder of rights, with a voice to claim them before the State, however this is not full citizenship. The leading of the investigation on the research subject was guided by a dialectical understanding of the discussion on Citizenship and Housing, which allowed capturing the essence of this relation that we intend to establish, based on the bibliographical review and field research carried out in a property development in phase 1 of the Housing Program Minha Casa Minha Vida.