Submitted by Eduarda Figueiredo (eduarda.ffigueiredo@ufpe.br) on 2015-03-10T18:15:31Z No. of bitstreams: 2 Rútilo - Dissertação.pdf: 4467264 bytes, checksum: 03a7b635f4dd003cdadf255540bf1e0c (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) / Made available in DSpace on 2015-03-10T18:15:31Z (GMT). No. of bitstreams: 2 Rútilo - Dissertação.pdf: 4467264 bytes, checksum: 03a7b635f4dd003cdadf255540bf1e0c (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2012-02-02 / CAPES / O presente trabalho trata da avaliação de material particulado em suspensão, tomando como caso de estudo a comunidade do Engenho Santana no município de Jaboatão dos Guararapes/PE, onde ocorre a extração de rochas graníticas para a produção de brita (Pedreira), bem como a produção de asfalto (Usina). De modo geral, os processos de lavra e beneficiamento mineral das pedreiras provocam a emissão de material particulado, aumentando sua concentração no ar atmosférico. Assim, o estudo visou à avaliação dos particulados, partindo do diagnóstico ambiental sobre a qualidade do ar em área residencial do entorno desses empreendimentos, localizada no Engenho Santana. A metodologia da pesquisa aplicada nas avaliações, qualitativa e quantitativa, consistiu em visitas técnicas no período de julho a setembro de 2011, através de entrevistas de campo e análise de documentos. Outrossim, também se fez uso da técnica de Avaliação de Impactos Ambientais – AIA para a identificação das fontes emissoras de particulados, assim identificando posteriormente uma usina de asfalto como outra importante fonte emissora poluente do ar atmosférico. Dos procedimentos para as medições de campo, fez-se uso de Amostradores de Grande Volume – AGVs (ou Hivol) para medições de Partículas Totais em Suspensão – PTS e de Materiais Particulados Inaláveis – MP10 no ar atmosférico, conforme postulados pela legislação vigente (Resolução CONAMA no 03/1990) e regido pelas normas técnicas da ABNT NBR no 9547/1997 e NBR no 13412/1995, respectivamente. Foram feitas medições que consistiram de um total de 12 amostras, sendo 6 para PTS com Limite de Tolerância – LT de 24 horas; em cujo padrão secundário (150μg/m3) foi ultrapassado em apenas uma vez (177.53μg/m3); e 6 para MP10, com o mesmo LT de 24 horas, em cujo padrão secundário (150μg/m3) foi inferior ao LT, atingindo o valor (73.66 μg/m3). Da análise da concentração de PTS, baseado no índice de qualidade do ar foi revelado que 66.6% das amostras foram classificadas como REGULAR, 16.7% como BOA e 16.7% como INADEQUADA; enquanto que, as concentrações de MP10, obtiveram o percentual de 16.7% como REGULAR e 83.3% como BOA. Assim, pôde-se constatar que a área de estudo apresenta concentrações de particulados (PTS e MP10) interferentes a qualidade do ar ambiente local. Portanto, os subsídios técnicos e ambientais apresentados neste trabalho servirão para o estabelecimento de medidas de monitoramento sistêmico e contínuo das emissões atmosféricas, visando à melhora da saúde e bem-estar da população local.

Title: Physical Self-perception of musical dancers Objectives: The main aim of the diploma thesis is to find out whether there are any gender differences in the physical self-concept of musical dancers. Methods: The thesis has a character of empirical and theoretical research and includes elements of quantitative research with an application of a survey. Specifically the thesis represents a cross-sectional study. A multidimensional questionnaire, the Physical self-perception profile was administered to a sample of N=40 musical dancers. The questionnaire consists of five subdomains including: sport competence, physical condition, physical attractiveness, physical strength and physical self-esteem. The t-test for two independent groups (men and women) was applied to test the group differences. Results: The results did not reveal any significant gender differences in physical self-concept between male and female musical dancers. Key words: PSPP, Physical Self-perception profile, dance, musical

Im Rahmen der Arbeit Molekularbiologische Charakterisierung des Glukose Phosphotransferase System Regulators MtfA aus Escherichia coli wurde das als Mlc titration factor A charakterisierte Protein MtfA auf genetischer, biochemischer und physiologischer Ebene näher charakterisiert. Mit Hilfe von gezielten Austauschen konservierter Aminosäuren konnte im aminoterminalen Bereich eine leuzinreiche Dimerisierungsdomäne und im carboxyterminalen Bereich eine Mlc Wechselwirkungsdomäne identifiziert werden. Letzteres konnte mit Hilfe von Di-Hybrid Studien unabhängig bestätigt werden. Die EIIBGlc Domäne des Glukosetransporters vermag Mlc zu binden. Durch die Membranassoziation des Transporters kann Mlc von seiner Operatorsequenz durch Titration entfernt werden. Lösliches EIIBGlc bindet ebenfalls an Mlc. Es löst aber keine Inaktivierung des Repressors aus. MtfA dagegen liegt cytoplasmatisch vor und inaktiviert Mlc vermutlich über die Inhibierung seiner Tetramerisierung. Eine parallele Expression von MtfA und EIIBGlc zeigte einen inhibitorischen Einfluss von EIIBGlc auf die Wechselwirkung von MtfA und Mlc. Physiologische Untersuchungen von MtfA weisen auf einen Einfluss des Proteins auf das Chemotaxisverhalten von E.coli hin. Darüber hinaus konnte ein relativ schwacher mtfA-Promotor nachgewiesen werden. Im Rahmen von Wachstumskompetitionsversuchen wurde ein Wachstumsvorteil des MtfA Wildtyps im Vergleich zur Mutante beim Wachstum auf Glukose und bei 42°C beobachtet. Außerdem stellte sich bei diesen Versuchen ein Unterschied in der Regulation von MtfA in den Stämmen K-12 und LJ110 heraus. Dieser konnte im Rahmen von RT-RT PCR Studien sowie mittels Western-Blot Analysen bestätigt werden. Die erfolgreiche Reinigung des Proteins ermöglichte den Nachweis der Dimerisierung in verschiedenen biochemischen Analysen, sowie die Herstellung spezifischer Antikörper.

Es wurde ein System zur chemischen Modifizierung von Einzelcysteinvarianten des PTS- Transporters EIICBGlc etabliert, mit dem durch unterschiedliche Zugänglichkeit von Markersubstanzen die Zuordnung der jeweiligen Cysteine in Hinblick auf die Cytoplasmamembran gelang. Für die Methode war es notwendig, eine cysteinfreies EIICBGlc zu konstruieren. Dieses trägt des Weiteren eine Mutation, die die Phosphorylierung des Substrats Glukose von dem Transport entkoppelt. Dies ist notwendig, da das Cystein 421, das für die Phosphorylierung des WT verantwortlich ist, in dem cysteinfreien Protein nicht mehr vorhanden ist. Die Transportfähigkeit der Mutanten konnte nachgewiesen werden. Die Ergebnisse des Cystein-Scannings, Daten über dieses Protein aus vorangegangenen Arbeiten, sowie ein Vergleich der Struktur des EIIChb aus B. cereus, ermöglichten die Erstellung eines neuen Modells des Proteins EIICBGlc. In diesem Modell enthält das Protein 10 transmembrane Helices. Die postulierte Substratbindetasche wird aus haarnadelartigen Strukturen gebildet. Die Lage funktioneller Mutanten in dem Modell wurde diskutiert. Die entkoppelte Mutation R203H des Proteins EIICBGlc wurde isoliert. Eine Charakterisierung in Bezug auf ihre Fähigkeit Mlc zu titrieren, zeigte, dass eine Bindung von Mlc in diesem Protein möglich ist. Eine Konformationsänderung, die die Wechselwirkung zu Mlc inhibiert, liegt daher in diesem Protein nicht vor. Es wurde außerdem gezeigt, dass keine Erweiterung der Substratspezifität in diesem Protein vorliegt.

Das E. coli EIIMtl gehört zu den PTSs (PEP-abhängiges Kohlenhydrat : Phosphotransferasesystem) und katalysiert den gekoppelten Transport von D-Mannitol in die Zelle. Es lässt sich in drei funktionelle Domänen unterteilen. Die membranüberspannende EIIC-Domäne mit der Substratbindestelle und der Transporterfunktion und die für die Übertragung der Phosphorylgruppe essentiellen hydrophilen Domänen EIIB und EIIA. Die exakte Topologie der EIIC-Domäne und deren Funktion bei Transport und Phosphorylierung sind bisher noch unklar. Zur Untersuchung der Funktion wurden Selektionssysteme konstruiert, um Mutanten aus Escherichia coli K-12 zu isolieren und charakterisieren, in denen der Transport von der Phosphorylierung entkoppelt ist. Diese Mutationen (E218A, E218V und H256P) wurden alle in der stark konservierten Schleife 5 des membrangebundenen Transporters EIIC gefunden, zwei davon in dem dort befindlichen GIH256E-Motiv. Die Suche nach Suppressormutationen ergab ebenfalls Mutationen in der Aminosäure 256 des GIHE-Motivs (P256A, P256Q und P256S). Die Kombination der verschiedenen Mutationen durch ortspezifische Mutagenese ergab unterschiedliche Effekte auf den Transport und der Phosphorylierung von D-Mannitol (Otte, S., Scholle, A., Turgut, S., and Lengeler, J.W. 2003. Mutations Which Uncouple Transport and Phosphorylation in the D-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14. J. Bacteriol. 185(7): 2267-2276). Bei der Untersuchung der Sekundärstruktur wurde die Lokalisation verschiedener Aminosäuren im EIIMtl durch die Methode des Cystein-Scanning überprüft. Mit Hilfe weiterer Sequenzuntersuchungen und unter Berücksichtigung weiterer Ergebnisse, konnte ein alternatives Sekundärstrukturmodell des EIICMtl vorgestellt werden, bei dem die Schleife 5 in die Membran gelegt wurde.

Military stigma is a heavy burden of social stigma internalized by veterans who are diagnosed with posttraumatic stress disorder (PTSD) during post-deployment psychological screening. PTSD is classified as a mental disorder associated with widespread reluctance to seek medical assistance. Among military veterans who suffer from combat-related posttraumatic stress (PTS), military stigma is considered a widespread problem. The purpose of this phenomenological study was to explore veterans' perceptions of various aspects of military stigma, including post-deployment psychological screening, the diagnosis of PTSD, and factors associated with reluctance to seek medical assistance for PTS. In-depth interviews were conducted with a convenience sample of 10 veterans of 2 recent operations in Iraq and Afghanistan. A modified form of labeling theory was applied to address the role of cultural stereotypes in stigma (societal and self-internalized), and the discriminatory factors associated with them. Multiple themes emerged, including a commonly held view that post-deployment health screenings are superficial, inconsistent, and ineffective procedures in which veterans feel the need to lie about their experience for fear of being stigmatized with a mental disorder. The findings confirm that the stigma associated with a diagnosis of PTSD perpetuates veterans' reluctance to seek help for PTS, which results in multiple personal and professional problems. Remedies recommended by these veterans included improved post-deployment medical screening procedures, reclassification of PTSD as a war injury instead of a mental disorder, and PTS-related stigma awareness training.

The purpose of this research is to explore preservice teachers’ attitudes and beliefs towards mathematics, mental and written computations, and mental computation anxiety, to investigate their use of different mental computation strategies using different approaches (i.e., Direct Teaching (DT) and Open-Approach (OA)) among the three different groups, and to identify how the use of preservice teachers’ mental computation strategies affects their flexibility regarding mental computation. The participants were preservice teachers (PTS). Three classes were used for this study: two classes in a mathematics class (Course A) for experimental groups and one class for the control group. One class from professional education courses was selected. A mixed methods design was used, more specifically, the Mathematics Attitude Survey (MAS) was administrated before and after intervention to examine PTS’ attitudes towards mathematics, mental and written computation, and mental computation anxiety. In addition, to determine whether there is any statistically significant difference among the three groups, the one-way analysis of variance (ANOVA) was used. Then, the MAS was analyzed descriptively. Next, a pre-and post-Mental Computation Test (MCT) was given to investigate PTS’ mental computation knowledge in relation to whole numbers, integers, and rational numbers (i.e., fractions, decimals, and percentages). A one-way analysis of covariance (ANCOVA) was conducted to determine if there were significant differences in mental computation performance among the three groups (i.e., DT, OA, and Control) with different instructions. Further, before and after intervention, face-to-face interviews were given to both the experimental and control groups to identify how they arrived at their answers. During interviews, 38 interviewees in the pre-interviews and 36 in the post-interviews for all groups participated. The interview items were selected from the pre-and post-MCT problems. Three levels of problems (i.e., high, medium, and low difficulty) for each operation were selected. The results of the MAS showed that with respect to the attitudes towards mathematics, PTS were generally shown positive attitudes towards learning mathematics and were aware of the importance of learning mathematics; however, in reality, about half of them did not want to spend time on learning or studying mathematics. In terms of PTS’ attitudes towards mental and written computation, PTS were aware that learning mental computation is more useful in real life situations and provides benefits in their mathematics learning. However, they do not feel comfortable and safe when using mental computation because of their lack of confidence and teaching abilities. For the mental computation, PTS showed slightly higher anxiety levels from pre-to post-tests. The findings of Mental Computation Test (MCT) revealed that there was a statistically significant difference in post-MCT scores between the different instructional groups when adjusted for pre-MCT scores. In particular, PTS using Open-Approach (OA) performed better than the PTS in the group using Direct Teaching (DT). The PTS in the control group performed worst. Significant differences between pre-and post-MCT performance were found among the three groups in solving multiplication, fraction, and decimal operations. The results of interviews suggest that there was an association between each interviewee’s quintile level and their flexibility in the use of the mental computation strategies. Regarding the whole number operation strategies, the results revealed that the interviewees in the middle and upper quintiles in both DT and OA used more than two different strategies with higher accuracy and were more likely to use the strategies. Interviewees in the middle and upper quintiles for the DT and OA groups were more likely to use the strategies that reflect efficient number facts or number-sense (e.g., Adding by place, Decomposing, & Compensation). The mental image of the Traditional method was frequently observed in the OA group. In contrast, for the lower quintiles, alternative strategies were not provided for both groups. The interviewees in the control group offered the smallest range of strategies. For the integer and rational operations, the interviewees in the DT group showed strategies that focused more on conceptual understanding. Surprisingly, the interviewees in the OA group were more likely to apply teacher-taught methods, including the Traditional method. The control group was not able to provide any alternative strategies. Plans for future research are set forth to add to the body of knowledge that exists regarding mental computation.

[pt] Este estudo pretende avaliar as influências do tráfego de rotina e da construção de um novo trecho da BR-040, entre Duque de Caxias e Petrópolis, na emissão e composição do material particulado (MP) da região. As obras foram iniciadas em 2014 e interrompidas em junho de 2016. Foram realizados o monitoramento tradicional de MP e biomonitoramento com Tillandsia usneoides e Tillandsia stricta transplantadas em seis locais de amostragem ao longo da rodovia. O Laboratório de Estudos de Poluição Atmosférica (LEPA) forneceu filtros contendo partículas totais em suspensão (PTS), coletadas entre 2014 e 2016. A espectrometria de massas com plasma indutivamente acoplado (ICP-MS) e a cromatografia iônica (CI) foram utilizadas para a caracterização química das PTS. Em 2014 foram observadas as maiores concentrações médias de PTS nos pontos próximos ao pedágio. Em 2016 não houve diferença nas concentrações de PTS entre períodos com obras e em sua ausência. O elemento majoritário nas PTS foi Fe e os maiores fatores de enriquecimento (FE) foram obtidos para Cu, Cd e Ni. Os íons em maior concentração foram NO3-e SO4-2, associados a emissões veiculares. O biomonitoramento, realizado em 2018, revelou enriquecimento de Mn, Zn e Cd na maioria dos locais. A T. stricta apresentou maiores FE, que pode estar relacionado com os locais de origem ou fisiologia das plantas. Uma análise prévia de biomarcadores de estresse oxidativo revelou correlações entre metalotioneína e Fe, Mn, Co, Cu, Ni e Zn, indicando concentrações maiores que as fisiológicas para esses metais. As concentrações de outros biomarcadores como glutationa reduzida e H2O2 também foram avaliadas. / [en] This study aims to evaluate the influence of daily traffic and road construction of a new stretch of BR-040, between Duque de Caxias and Petrópolis, in the emission and composition of the particulate matter (PM) of the region. The construction works started in 2014 and were interrupted in June 2016. The traditional monitoring of PM and biomonitoring with transplanted Tillandsia usneoides and Tillandsia stricta were conducted at six sampling sites along the highway. The Laboratory of Atmospheric Pollution Studies (LEPA) provided filters containing total suspended particulates (TSP), collected between 2014 and 2016. inductively coupled plasma mass spectrometry (ICPMS) and ion chromatography (IC) were used to the chemical characterization of TSP. In 2014, the highest average concentrations of TSP were observed at points close to the toll. In 2016 there was no difference in TSP concentrations between periods with works and in their absence. The major element in the PTS was Fe and the greatest enrichment factors (FE) were obtained for Cu, Cd and Ni. The ions in higher concentrations were NO3 -and SO4-2 , associated with vehicular emissions. Biomonitoring, conducted in 2018, showed enrichment of Mn, Zn and Cd in most sites. T. stricta presented higher FE, which may be related to the sites of origin or physiology of the plants. A previous analysis of oxidative stress biomarkers revealed correlations between metallothionein and Fe, Mn, Co, Cu, Ni and Zn, indicating higher concentrations than the physiological ones for these metals. Concentrations of other biomarkers reduced glutathione and H2O2 were also evaluated.

No description available.

Previous literature has demonstrated that supplemental magnesium may have a prophylactic effect on noise induced hearing loss (NIHL). The purpose of this study was to replicate the findings of a previous investigation concerning the beneficial effects of supplemental magnesium on TTS in humans. Forty participants with normal hearing were divided into two groups, experimental and control. The experimental group received a single, 150 mg dose of supplemental magnesium and the control group received a placebo. After an hour in a quiet environment, all participants were exposed to a narrowband noise centered at 2000 Hz and presented at 105 dB SPL. Audiometric thresholds were measured for the frequency range 250 Hz - 8000 Hz prior to the noise exposure, and then immediately, 30 minutes, and 60 minutes post-noise exposure. Both groups experienced TTS at and above 2000 Hz. The largest TTS was observed immediately following the noise exposure in both groups. There was no significant difference in TTS between the experimental group and the control group. It was concluded that a single dose of 150 mg of supplemental magnesium was not effective in reducing or preventing TTS.