La rénovation et la réhabilitation énergétique du parc de bâtiment est un des grands défis identifiés pour les décennies à venir. Devant cet impératif d'une rénovation d'ampleur du parc construit, les solutions techniques utilisant des ossatures rapportées sont employées de plus en plus fréquemment car elles permettent de combiner performance thermique, renouvellement esthétique et ajouts fonctionnels. Une des difficultés dans l'amélioration et l'automatisation des projets de rénovation est liée à la connaissance de la géométrie du bâti existant. Des plans de l'état existant ne sont pas toujours disponibles, et dans le cas échéant, ne sont pas forcément exacts en raison de modifications apportées au bâti et non documentées ou d'écarts initiaux entre les plans et la réalisation. Après une étude bibliographique des méthodes existantes détaillées dans le chapitre 1. Les travaux menés dans le cadre d'une collaboration entre le CRITT Bois et le CRAN ont permis de développer une méthode automatique de reconstruction 3D du modèle de bâtiments à partir de nuages de points obtenus par scanner LASER terrestre. La méthode proposée se décompose en trois phases principales. La première phase détaillée dans le deuxième chapitre, consiste à segmenter le nuage de points en plusieurs plans représentants les façades du bâtiment. L'exploitation de la colorimétrie durant la phase de segmentation du nuage de points permet une réduction importante de la complexité de l'algorithme de segmentation géométrique. L'approche consiste à effectuer dans un premier temps une classification préalable du nuage de points en se basant sur les informations colorimétriques de chaque point. Puis, dans un second temps, il s'agit d'effectuer une segmentation géométrique du nuage de points en utilisant un algorithme de segmentation robuste (RANSAC). Le chapitre 3 présente la deuxième phase qui consiste à modéliser le pas d'échantillonnage de la surface à partir duquel on définit le seuil d'extraction des points de contours. Le but étant d'améliorer la fiabilité d'extraction des points de contours, ainsi que l'approximation de l'erreur sur le modèle. Le chapitre 4 détaille les principales étapes de la reconstruction d'un modèle filaire. Dans un premier temps les régions définies par les points de contours sont classées en Régions d'Irrégularités (RI), Régions d'Eléments Architecturaux (REA) comme les fenêtres et Régions de Façade (RF) représentées par les contours extérieurs de la façade. La deuxième étape consiste à modéliser les différentes régions, par un maillage de Delaunay pour les RI, et des polyèdres pour les RF et REA. La dernière étape calcule une approximation de l'erreur sur le modèle. Les tests de fiabilité de la méthode ont été réalisés sur des chantiers réels conduits par des industriels de la construction et de la rénovation. Il en ressort que la qualité de la reconstruction 3D reste fortement dépendante des facteurs d'acquisition ainsi que de la surface numérisée. L'approximation de l'erreur de modélisation permet ainsi de prévoir à l'avance les erreurs sur le modèle CAO. / The renovation and the improvement of the energy efficiency of existing housing stock is one of big challenges identified for coming decades. In front of this imperative, timber based elements for building renovation are more and more used due to their substantial improvement of the building insulation, aesthetic renewal and functional additions. However, this technology faces some difficulties, one of them is the improvement of the renovation projects automation, which is bound to the knowledge of the existing built geometry. The plans representing the existing state of the building are not always available, and if so, they may be not exact, because the modifications made on the building are usually undocumented. After a literature review of existing methods which are detailed the first chapter. The work within the framework of cooperation between the CRITTBois and CRAN have allowed to develop an automatic method for 3D building CAD model reconstruction from point clouds acquired by a terrestrial LASER scanner. The proposed method is composed of three main phases. The first one detailed in the second chapter, consists in segmenting the point cloud into planar patches representing the building facades. To decrease the segmentation algorithm complexity, the colorimetric information is also considered. The approach consists in making a colorimetric classification of the point cloud in a first step, then a geometrical segmentation of the point cloud using a robust segmentation algorithm (RANSAC). The third chapter presents the second phase of our approach consists in surface sampling steps modeling and boundary point extraction. Here, we consider a local threshold defined according to the approximated surface sampling steps. The aim of considering local threshold is to improve the reliability of the boundary point extraction algorithm and approximating the CAD model error. The last chapter presents the main three steps of the boundary model reconstruction method. The first step consists in classifying the regions defined by their boundary points into three types of regions: Irregularity Region (IR), Architectural Element Region (AER) as windows, and Facades Regions (FR) which represent the building facades defined by their outer boundaries. The second step consists in modeling these regions considering a Delaunay triangulation for the IR and a polyhedral model for the AER and the FR. The third step consists in making an approximation of the error in the model. The method reliability tests were conducted on real projects; they were performed by industrial construction and renovation professionals. The tests show that the quality of the 3D reconstruction remains strongly dependent to the acquisition factors and the scanned surface properties. Also, the approximation of the modeling error can predict in advance the errors on the CAD model.

Les travaux décrits dans ce mémoire ont pour objectif d’une part le développementd’une nouvelle famille de protéines artificielles et d’autre part la création de nouveaux sitesde fixation spécifiques dans ces protéines. L’objectif général était de développer une approchegénérale permettant d’obtenir rapidement des protéines reconnaissant toute macromoléculecible choisie. On peut voir ces protéines artificielles spécifiques comme des sortes d’anticorpsartificiels pour leur spécificité et leur affinité mais dont les propriétés physiques : stabilité,solubilité, efficacité d’expression, insensibilité à l’agrégation sont nettement plus favorablesque celles des anticorps et de leur dérivés.Le premier chapitre, présente la conception et la construction d’une bibliothèque deprotéines artificielles dite de première génération où les protéines sont formées par larépétition d’un motif idéalisé à partir d’une famille de motifs naturels appelés HEAT repeats.Toutes les protéines de la bibliothèque, dénommées αRep, sont conçues pour avoir la mêmearchitecture générale mais diffèrent les unes des autres par le nombre de motifs et par laséquence dans certaines positions rendues variables au sein de chaque motif. Cette banquenous a permis de valider l’architecture αRep choisie : Les protéines s’expriment sous formesoluble, sont très stables et adoptent la structure secondaire et tertiaire attendue quel que soitla séquence des positions hypervariables. Le second chapitre présente alors les approchessuivies pour l’amélioration de la qualité et de la diversité de la bibliothèque et a conduit à laconstruction d’une bibliothèque d’αRep de deuxième génération. Cette dernière bibliothèque(2 .1) repose sur le même schéma général mais contient une diversité ayant été optimiséelors de la conception puis améliorée expérimentalement par une procédure dite deFiltration/shuffling. Cette bibliothèque très diverse (1.7*109 clones indépendants) a été alorsexploitée pour y rechercher, par des méthodes d’exposition sur phages, de nouvelles αRepreconnaissant des protéines cibles préalablement choisies. L’ensemble des résultats montretrès clairement que des αRep reconnaissant spécifiquement, avec une affinité élevée, desprotéines cibles choisies arbitrairement peuvent être effectivement obtenues. Les structurestridimensionnelles de plusieurs complexes formés entre les αRep et leur cible a été résoluepermet de comprendre la nature et l’organisation précise de ces capacités de reconnaissancemoléculaire nouvellement créées. / The main objective of this work was to design, produce and characterize a new familyof artificial proteins and to introduce new tailored specific binding sites within this structuralframework. Our general goal was to develop method allowing to rapidly generate newprotein binding specifically to any predefined target macromolecule. Binders based onartificial proteins can be viewed as antibody-like molecules but due to their different structurehave more favorable physical properties (expression, solubility, folding efficiency, stability)than antibodies and derivatives.The design and experimental assembly of a first generation artificial protein library isdescribed in part I. Proteins of this library are made by a repetition of a motif idealized from afamily of natural protein repeats (HEAT repeat). These artificial proteins, named αRep, havethe same general fold but the number of the repeated motif vary from protein to protein.Furthermore, a set of positions of each motif is highly variable within the library. Proteinisolated from this first generation library are well expressed, soluble, extremely stable andwere shown to have the designed secondary and tertiary structure.The methods used to improve the diversity and the experimental quality of the protein libraryare described in the second part of this thesis and have allowed us to create a secondgeneration αRep library. This library is based on the same general scheme but its diversitywas optimized by an improved design and experimental procedures known as filtration/shuffling.This highly diverse second generation library (1.7*109 independent clones) was usedto select variants with tailored binding specificities using phage display method. The resultsclearly show that news αReps binding tightly and specifically a range of arbitrarily definedprotein targets can be efficiently selected. The tertiary structure of complexes between αRepand their cognate target molecule were solved and allow to analyze the nature and detailedorganization of this newly engineered molecular recognition capacities.

Made available in DSpace on 2014-12-17T14:36:37Z (GMT). No. of bitstreams: 1 FranciscoATS_DISSERT.pdf: 2461406 bytes, checksum: 8103c11dd552d0c65bf3fb4420f4c09f (MD5) Previous issue date: 2012-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / El presente estudio tiene por objetivo, analizar las pr?cticas pol?ticas-intelectuales de Augusto Tavares de Lyra, perteneciente a una elite que gobern? el estado de R?o Grande del Norte de Brasil, durante los dos primeros decenios de la Rep?blica Vieja , de 1889 a 1918. El recorte temporal considerado tiene inicio al final del siglo XIX, en 1872, a?o de su nacimiento, hasta el a?o 1958, cuando falleci? a los casi ochenta y seis a?os de vida. Mientras tanto, analizaremos vivencias y pr?cticas de Tavares de Lyra como hombre p?blico a partir de los documentos investigados. Utilizamos como soporte principal art?culos, reportajes, discursos y libros escritos por sus contempor?neos. Observamos que las fuentes documentales, tales como mensajes, leyes y decretos gubernamentales, bibliograf?as sobre el per?odo evocado y el archivo del intelectual Tavares de Lyra. Entendemos que, aunque fuera un pol?tico de pr?cticas liberales y empe?ado en reformar el sistema educativo brasilero, ?l fue fruto de un instante de la pol?tica nacional, que privilegi? pocos n?cleos familiares en detrimento de la democracia descrita solamente en la ley, y que por eso, pose?a comprometimiento con las pr?cticas de la Primera Rep?blica. Su legado reside en una obra literaria ligada directamente al Instituto Hist?rico y Geogr?fico Brasileiro y a los estudios realizados, en cuanto jurista y economista, conocedor de los problemas que aflig?an Brasil en esa ?poca / O presente estudo tem por escopo analisar as pr?ticas pol?tico-intelectuais de Augusto Tavares de Lyra, pertencente a uma elite que governou o Rio Grande do Norte, durante os dois primeiros dec?nios da Rep?blica Velha de 1889 a 1918. O recorte temporal firmado tem in?cio no final do s?culo XIX, em 1872, ano de seu nascimento, at? o ano de 1958, quando faleceu aos oitenta e seis anos incompletos, no Rio de Janeiro. No entanto, analisaremos as viv?ncias e pr?ticas de Tavares de Lyra como homem p?blico a partir de documentos pesquisados. Utilizamos como principal suporte artigos, reportagens, discursos e livros escritos por seus contempor?neos. Observamos que as fontes documentais, tais como mensagens, leis e decretos governamentais, bibliografias acerca do per?odo evocado e o arquivo do intelectual Tavares de Lyra. Entendemos que embora pol?tico de pr?ticas liberais e empenhado em reformar o sistema educacional brasileiro, ele foi fruto de um instante da pol?tica nacional que privilegiou poucos n?cleos familiares em detrimento da democracia descrita somente na lei e que, por isso, possu?a comprometimentos com as pr?ticas da Primeira Rep?blica. Seu legado reside em uma obra liter?ria ligada diretamente ao Instituto Hist?rico e Geogr?fico Brasileiro e aos estudos realizados, enquanto jurista e economista, conhecedor dos problemas que afligiam o Brasil da ?poca

Made available in DSpace on 2014-12-17T14:36:43Z (GMT). No. of bitstreams: 1 SebastiaoAM_DISSERT.pdf: 5798459 bytes, checksum: cd3de06a83db2c6d14cf09b021a47451 (MD5) Previous issue date: 2012-08-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / El trabajo que aqu? se presenta tuvo como objetivo investigar una instituci?n escolar en la ciudad de Macau / RN. Esta instituci?n, que fue innovadora en su contexto social y pedag?gico, fue pesquisada a la luz de los par?metros de la historia cultural. Una escuela que fue creada en el siglo XX, en el a?o de 1923, cuando el pa?s a?n viv?a la efervescencia de la implantaci?n del nuevo ordenamiento pol?tico - la Rep?blica. Elegimos como objeto de estudio las fiestas escolares, que tuvo lugar en esta instituci?n durante el periodo 1949-1962, procurando aclarar c?mo ocurr?a la realizaci?n de estas fiestas y c?mo fueron fundamentales para el funcionamiento de las relaciones pol?ticas, sociales y culturales en el ?mbito escolar. El an?lisis y la interpretaci?n de las fuentes documentales escolares, los documentos de otras instituciones, las entrevistas abiertas, la informaci?n oral, la legislaci?n de la educaci?n y de las escuelas, los peri?dicos y documentos oficiales de la ?poca relacionados con la tem?tica y que formaron el corpus documental, se basaron por obras de Souza (1998), Juli? (2001), Castro (1997), Kossoy (2001) Saviani (2005), Escolano (1992) y Carvalho (1990). Por tratarse de una instituci?n de ense?anza, el an?lisis central es la cultura escolar y la historia de la educaci?n brasile?a es el campo de la investigaci?n, haciendo una relaci?n de las fiestas escolares. En el relato, se reconstituyen las festividades c?vicas, solemnes y recreativas, adem?s de los elementos de formaci?n que se evidencian en el discurso de la educaci?n moderna. Para ello, buscamos entender que, en las pr?cticas cotidianas de la escuela, hab?a un m?todo de actuaci?n que segu?a las directrices del Departamento de Educaci?n. Estas directrices eran dadas por medio de las normas y decretos editados y se adentraban en el discurso en torno de las iniciativas emprendidas para difundir las nuevas pr?cticas pedag?gicas, y dentro de ellas, en el momento apropiado, los profesores dedicaban un tiempo de sus clases para explicar acerca de los d?as festivos y sus organizaciones. Estas constataciones fueron certificadas por la documentaci?n listada y por las entrevistas abiertas, y nos se?al? para el modelo escolar propugnado por el Grupo Escolar Duque de Caxias, que era fruto del nuevo orden republicano / O trabalho aqui apresentado teve como objetivo investigar uma institui??o escolar na cidade de Macau/RN. Essa institui??o que foi inovadora, no seu contexto social e pedag?gico, foi pesquisada ? luz dos par?metros da hist?ria cultural. Uma escola que foi criada no in?cio do s?culo XX, no ano de 1923, quando o pa?s ainda vivia a efervesc?ncia da implanta??o da nova ordem pol?tica nacional a Rep?blica. Elegemos como objeto de estudo as festas escolares, que aconteciam nesta institui??o de ensino, no per?odo de 1949 a 1962, procurando esclarecer como ocorria a realiza??o dessas festas e como foram fundamentais para o funcionamento das rela??es pol?ticas, sociais e culturais no entorno do educand?rio. A an?lise e interpreta??es das fontes documentais escolar, dos documentos das outras institui??es, das entrevistas abertas, informa??es orais, legisla??o da educa??o e dos educand?rios, jornais da ?poca e documentos oficiais, relacionados ? tem?tica e que formaram o corpus documental, nortearam-se pelas obras de Souza (1998), Juli? (2001), Castro (1997), Kossoy (2001) Saviani (2005), Escolano (1992) e Carvalho (1990). Por se tratar de uma institui??o de ensino, a an?lise central ? cultura escolar e a hist?ria da educa??o brasileira ? o campo de investiga??o, elencando as festas escolares. Na narrativa, reconstituem-se os eventos festivos nas categorias c?vicas, solenes e recreativas, al?m de elementos de forma??o que se evidenciam no discurso da educa??o moderna. Para isso, buscamos compreender que, nas pr?ticas cotidianas da escola, havia um m?todo de atua??o que seguia as orienta??es do Departamento de Educa??o. Tais orienta??es eram dadas atrav?s das normas e decretos editados e adentravam-se nos discursos em torno das iniciativas empreendidas para difundir as novas pr?ticas pedag?gicas, e dentro delas, nos momentos adequados, as professoras dedicavam um tempo das suas aulas para explanar sobre as datas festivas e suas organiza??es. Essas constata??es foram certificadas pela documenta??o elencada e pelas entrevistas abertas, e nos apontou para o modelo escolar propugnado pelo Grupo Escolar Duque de Caxias, que era fruto da nova ordem republicana

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-18T13:33:35Z No. of bitstreams: 2 Dissertação - Bruna Alicia Rafael de Paiva - 2014.pdf: 5696996 bytes, checksum: 7e1928a13da15787585830bd9a4f4ae1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-18T13:34:11Z (GMT) No. of bitstreams: 2 Dissertação - Bruna Alicia Rafael de Paiva - 2014.pdf: 5696996 bytes, checksum: 7e1928a13da15787585830bd9a4f4ae1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-04-18T13:34:11Z (GMT). No. of bitstreams: 2 Dissertação - Bruna Alicia Rafael de Paiva - 2014.pdf: 5696996 bytes, checksum: 7e1928a13da15787585830bd9a4f4ae1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2014-02-25 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The Common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli (Xap) and Xanthomonas fuscans subsp. fuscans (Xff), is one of most important bacterial etiology diseases from the common bean plant. This pathogen causes a great loss in the production, mainly in the “water” harvest, when the environmental conditions are helpful to the development of this disease and to its dissemination. Among the strategies about integrated handling of diseases, the genetic resistance is the main measure of control and, for obtaining some cultivate with lasting resistance and of notorious specter, it is important to the know the genetic pathogen diversity. The present work has the goal of studying the diversity and the genetic structure around and inside the population of Xap and Xff using the markers rep-PCR; to connect the genetic pathogen diversity with its geographical distribution inside Brazilian producing common bean plant regions. There were obtained 42 Xap and Xff isolated and four Xanthomonas isolated not pathogens of to the bean plant, originated in the States of São Paulo, Goiás, Paraná and Rio Grande do Sul. The genetic profiles obtained by primers BOX, ERIC and REP produced 12 haplotipes matched (HC), where the HC 3, specific xff, was more frequent in PR and GO, and was not found in RS. The dendrogram produced through the analysis showed that Xap, Xff and isolated not pathogens are genetically different. In the analysis of genetic structure, the total genetic diversity (Ht), among three populations was 0,2385 revealing that there was variability from a population to the other one. Higher values of the Shannon rate (0,3648) show that Xap population is more different genetically. From a total of 51 locos, 94,12 % are polymorphic, inside Xap population they are all polymorphicand inside Xff, only 18,18 % are polymorphic. The genetic differentiation coefficient (Gst = 0,5194) revealed diversity inside and among the patogenic populations, confirming AMOVA’s result, where 51,98 % of the diversity was among the populations and 48,06 % inside them. Then, the technique helped how to find differences between Xap and Xff and measure the values of genetic diversity among and inside populations. The results of this study give us much information about the pathogens diversity, which is considered useful to identify and to characterize the resistant germoplasm in a more efficient way. / O crestamento bacteriano comum, causado por Xanthomonas axonopodis pv. phaseoli (Xap) e Xanthomonas fuscans subsp. fuscans (Xff), é uma das doenças de etiologia bacteriana mais importantes do feijoeiro-comum. Este patógeno provoca grandes perdas na produção, principalmente na safra das “águas”, quando as condições ambientais são favoráveis ao desenvolvimento da doença e à disseminação do patógeno. Dentro das estratégias do manejo integrado de doenças, a resistência genética é a principal medida de controle, e para a obtenção de cultivares com resistência duradoura e de amplo espectro é necessário o conhecimento da diversidade genética do patógeno. O presente trabalho teve como objetivos, estudar a diversidade e a estrutura genética entre e dentro das populações de Xap e Xff usando marcadores rep-PCR; relacionar a diversidade genética do patógeno com sua distribuição geográfica dentro das regiões produtoras de feijoeiro-comum no Brasil. Foram obtidos 42 isolados de Xap e Xff, e quatro isolados de Xanthomonas não patogênicas ao feijoeiro, oriundos dos estados de São Paulo, Goiás, Paraná e Rio Grande do Sul. Os perfis genéticos obtidos pelos primers BOX, ERIC e REP geraram 12 haplótipos combinados (HC), em que o HC 3, específico de Xff, foi mais frequente nos estados do PR e GO , porém não foi identificado no RS. O dendrograma gerado através da análise conjunta mostrou que Xap, Xff e isolados não patogênicos foram geneticamente distintos. Na análise de estruturação genética, a diversidade genética total (Ht) entre as populações foi de 0,2385, mostrando que houve variabilidade entre as populações. Valores mais altos do índice de Shannon (0,3648) revelam que a população de Xap é mais diversa geneticamente. De um total de 51 locos em todas populações, 94,12% foram polimórficos, sendo que, dentro de Xap todos foram polimórficos, e dentro de Xff apenas 18,18% foram polimórficos. O coeficiente de diferenciação genética (GST = 0,5194) revelou diversidade tanto dentro como entre as populações patogênicas, confirmando o resultado obtido pela AMOVA, em que 51,98% da diversidade está distribuída entre as populações e 48,06% dentro destas. Portanto, o uso da técnica rep-PCR permitiu diferenciar Xap e Xff e mensurar os valores de diversidade genética entre e dentro de populações. Os resultados deste estudo fornecem informações sobre a diversidade dos patógenos, o que auxiliará para melhor identificar e caracterizar o germoplasma resistente.

O operon pst de Escherichia coli é formado pelos genes pstS, pstC, pstA, pstB e phoU. Os quatro primeiros genes codificam proteínas que compõem um sistema de transporte do tipo ABC denominado Pst. O sistema Pst, junto com a proteína PhoU participa da repressão dos genes do regulon PHO. A transcrição dos genes do operon pst é induzida pela carência de fosfato inorgânico (Pi). Neste trabalho, o padrão de transcrição do operon pst foi analisado. A existência de um transcrito primário instável foi comprovada através de uma nova técnica de RT-PCR. O papel da RNase E na degradação do mRNA de pst foi demonstrado. A análise das seqüências intergênicas do operon revelou a importância da seqüência REP (Repetitive Extragenic Palindrome) localizada na região intergênica entre pstS e pstC na estabilização da mensagem de pstS. As seqüências localizadas a 5\' dos genes pstC, pstB e phoU também foram analisadas e demonstraram uma atividade promotora fraca, que resulta na síntese de transcritos dos genes distais de pst. / The pst operon of Escherichia coli consists of the genes pstS, pstC, pstA, pstB and phoU. The four proximal genes of the operon encode the proteins of the ABC-type Pi phosphate (Pi) transporter Pst.The Pst system, together with the PhoU protein, also acts as a negative regulator of the PHO regulon. Transcription of the pst genes is induced by Pi starvation. The present study describes the transcription pattern of the pst operon. The existence of an unstable primary transcript was confirmed by using an improved RT-PCR protocol. The role of RNase E in pst transcript decay was demonstrated.Analysis of the operon intergenic regions revealed the role of a REP sequence (Repetitive Extragenic Palindrome) located between pstS and pstC in pst mRNA stability. The regions upstream of pstC, pstB and phoU displayed promoter activity. Transcription from these internal promoters resulted in a small amount of mRNAs corresponding to the pst distal genes.

Chronic infection with P. aeruginosa is expected in patients with cystic fibrosis (CF), but the ability to delay, prevent, or better manage infection with multiply-resistant P. aeruginosa (MRPA) can potentially increase quality of life and extend survival. The Texas Children’s Hospital CF Care Center has identified an endemic MRPA strain (dominant clone), and this study aimed to identify risk factors for acquisition of the clone as well as determine differences in patient outcome associated with subsequent infection with the clone. The study included 71 patients with CF with documented MRPA infection. Designation of patients as members of the dominant clone or a non-dominant clone group was based on molecular typing by rep-PCR of MRPA isolates from respiratory cultures. Patient data was collected from Port CF, the national patient registry of the CF Foundation. Patient demographic information and clinical parameters prior to MRPA infection were analyzed by logistic regression as potential risk factors. Differences in patient outcome including change in BMI, change in FEV1, and hospitalization rate were evaluated by MANOVA. Recent hospitalization (< 90 days) was a statistically significant (p = 0.035) risk factor for acquisition of the dominant clone. Patients hospitalized < 90 days prior to MRPA diagnosis were four times more likely to be infected with the dominant clone, and patients hospitalized 91-180 days prior were almost three times more likely. Increased hospitalization rates were seen in the dominant clone group both pre- (11 more days/year) and post-infection (14 more days/year) as compared to the non-dominant clone group. Patients infected with the endemic strain exhibited poorer outcomes in terms of nutritional status (3.73% decrease/year in BMI %ile) and lung function (3.7% decrease/year in FEV1 %ile). Significant overlap in hospitalization episodes of patients known to be infected with the dominant clone and patients subsequently infected with the dominant clone was observed. Recent hospitalization was a significant risk factor for infection with the dominant MRPA clone, and following infection, patients infected with the endemic strain exhibited declines in nutritional status and lung function and increased hospitalization rates. The results suggest potentially increased virulence and transmissibility of the endemic MRPA strain.

The output of the National economy of the Czech Republic is conditioned by a sum of important factors. There are sectors, which increased power during the last two decades, mainly due to expansion of modern technologies and knowledge workers. One of this is Construction. Construction is specific to its position in the economy and in particular is characterized by the greatest seasonality ever. However, this is not a problem for statistical analysis, rather a benefit. Modern approaches allow us to analyze seasonal fluctuations. From selected data we are able to construct evolutionary forecasts. The work will be performed for the most important indicators in the Czech Construction. The outcome of the paper will be conditional forecasts of these indicators. It will also make analyze of the relationship between these indicators and other variables that might affected it. The work is practical application of stochastic modeling approach by Box and Jenkins, augmented by more modern approaches, such as verification of Granger causality and co-integration and testing of seasonal unit roots by Hylleberg et al.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-07-04T18:10:09Z No. of bitstreams: 1 TES_SAUL_ESTEVAM_FERNANDES_COMPLETO.pdf: 3375408 bytes, checksum: 27f32bc3a1409a358307936c83c75258 (MD5) / Made available in DSpace on 2017-07-04T18:10:09Z (GMT). No. of bitstreams: 1 TES_SAUL_ESTEVAM_FERNANDES_COMPLETO.pdf: 3375408 bytes, checksum: 27f32bc3a1409a358307936c83c75258 (MD5) Previous issue date: 2017-03-23 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The present study discusses how the mechanisms for resolving interstate boundary issues were metamorphosed during the First Republic through debates in various institutions, such as the National Congress, the Senate, the Federal Supreme Court (STF), among others. We have noticed that, in the beginning, thought from the subordinations of the state interests to a greater interest, the national, the resolution devices of the interstates boundaries issues have been dissolved throughout the politics. However, the gaps found in the elaboration of the constitution of 1891 were of fundamental importance for the judicialization of those devices. We also have noticed that the process of judicialization started at the STF ended up metamorphosed in other ways, because of the long legal proceedings of brazilian justice, giving subterfuges on recognizing and strenghtening of the arbitral tribunals, as well as in the importance attributed to the national executive through the intermediary of the President of the Republic or emissaries linked to it. / O presente estudo discute como os dispositivos de resolu??o das quest?es de limites interestaduais foram se metamorfoseando durante a Primeira Rep?blica atrav?s dos debates em diversas institui??es, como Congresso Nacional, Senado Federal, Supremo Tribunal Federal (STF), dentre outras. Perceberemos que, inicialmente, a partir da subordina??o dos interesses estaduais pelo interesse maior, o nacional, as resolu??es das quest?es de limites foram pensadas para serem dirimidas atrav?s da pol?tica. Contudo, as brechas dispostas na produ??o da Constitui??o de 1891 foram de fundamental import?ncia para que houvesse a judicializa??o dos dispositivos. Ainda perceberemos que o processo de judicializa??o iniciado no STF acabou se metamorfoseando em outro meio, pois os demorados tr?mites da justi?a brasileira acabaram dando subterf?gios no reconhecimento e fortalecimento dos Tribunais Arbitrais, assim como na import?ncia atribu?da ao Executivo Nacional por meio da intermedia??o do Presidente da Rep?blica ou de emiss?rios vinculados ao mesmo.

Submitted by PPG Hist?ria (historia-pg@pucrs.br) on 2018-05-30T12:03:50Z No. of bitstreams: 1 Tese Tassiana Saccol vers?o final.pdf: 1741370 bytes, checksum: e244e9edc4e64fae33bd576748c642e7 (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-06-08T13:08:15Z (GMT) No. of bitstreams: 1 Tese Tassiana Saccol vers?o final.pdf: 1741370 bytes, checksum: e244e9edc4e64fae33bd576748c642e7 (MD5) / Made available in DSpace on 2018-06-08T13:38:23Z (GMT). No. of bitstreams: 1 Tese Tassiana Saccol vers?o final.pdf: 1741370 bytes, checksum: e244e9edc4e64fae33bd576748c642e7 (MD5) Previous issue date: 2018-03-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This thesis aims to analyze how some of the main divisions of Rio Grande ? Republican Party take place. Taking as its object the first and second great dissidents ? which occurred, respectively, in 1890/91 and 1907 ? we seek to evaluate which motivations led to the rupture of leaders with the party and, thus, what was being effectively disputed in each of these conjunctures of party and political play. The individual journeys of some of the main dissidents were the guiding thread of our investigation. From the identification of the debates and episodes in which each one of these individuals were involved, the insufficiency of the explanations based on supposed ideological rivalries could be verified, proposing, in contrast, disputes over personal interests and spaces of power as a common motive for these dissidents. The empirical material researched consists mainly of press editorials and private correspondences, works based on an approach that favors notions as faction and chain of leader-followers, and which aims to better understand the logics pertaining to Rio Grande do Sul politics of the late nineteenth and early twentieth century. / El objetivo de esta tesis es analizar c?mo se procesaron algunas de las principales escisiones del Partido Republicano Rio-Grandense. Tomando como objeto la primera y la segunda gran disidencia ? ocurridas, respectivamente en 1890/91 y 1907 ? buscamos evaluar qu? motivaciones ocasionaron la ruptura de los l?deres con el partido y, siendo as?, qu? seria lo que de hecho se estaba disputando en cada una de esas coyunturas del juego partidista y pol?tico. Los recorridos individuales de algunos de los principales disidentes fueron el hilo conductor de nuestra investigaci?n. A partir de la identificaci?n de los debates y episodios en que cada uno de esos individuos estuvieron involucrados, se pudo constatar la insuficiencia de las explicaciones fundamentadas en supuestas rivalidades ideol?gicas, proponiendo en contrapartida, disputas por intereses personales y espacios de poder como motivo com?n de esas disidencias. El material emp?rico investigado se compone principalmente de editoriales de la prensa y de correspondencias particulares, trabajados a partir de un enfoque que privilegia nociones como facci?n y cadena de l?deres-seguidores, y que pretende mejor comprender las l?gicas relativas a la pol?tica rio-grandense de fines del siglo XIX y principios del siglo XX. / Esta tese tem como objetivo analisar como se processaram algumas das principais cis?es do Partido Republicano Rio-Grandense. Tomando como objeto a primeira e a segunda grande dissid?ncias ? ocorridas, respectivamente, em 1890/91 e 1907 ? buscamos avaliar quais motiva??es ocasionaram a ruptura dos l?deres com o partido e, sendo assim, o que estava sendo disputado efetivamente, em cada uma dessas conjunturas do jogo partid?rio e pol?tico. Os percursos individuais de alguns dos principais dissidentes foram o fio condutor de nossa investiga??o. A partir da identifica??o dos debates e epis?dios, em que cada um desses indiv?duos esteve envolvido, p?de-se constatar a insufici?ncia das explica??es fundamentadas em supostas rivalidades ideol?gicas, propondo em contrapartida, disputas por interesses pessoais e espa?os de poder como motivo comum dessas dissid?ncias. O material emp?rico pesquisado comp?e-se, principalmente, de editoriais da imprensa e de correspond?ncias particulares, trabalhados a partir de uma abordagem que privilegia no??es como fac??o e cadeia de l?deres-seguidores, e que visa melhor compreender as l?gicas atinentes a pol?tica rio-grandense de fins do s?culo XIX e princ?pios do s?culo XX.