A growing interest for Cannabidiol (CBD), a component of Cannabis Sativa, has occurred over the past years. The medical potential of the component is yet to be better characterized, as its effects on sleep, and in particular memory, are to date not well understood or consistently characterized. This master thesis project focuses on analysing the effect of CBD on an anaesthesia-induced sleep-like state in rats, and its effects on the hippocampal sharp-wave-ripples, which have been shown to be associated with memory replay during sleep, and hence system consolidation. The hippocampus and prefrontal cortex, the two structures involved in memory consolidation, were recorded in 19 rats, split in two groups (CBD and vehicle). From these recordings, an automated sleep scorer using principal component analysis was developed to obtain the animals’ hypnograms, which were analysed to study sleep-like structure. From the recordings of the hippocampal pyramidal layer, and an additionnal layer deeper under it, respectively ripples and sharp waves were detected in all animals, and characterized for each group. We observed and demonstrated that CBD changes the sleep-like structure by shortening both REM and NREM bouts, resulting in an increase in transitions between both states. Additionally, we observed that, although ripples are not significantly different between both groups, sharp waves tend to be smaller among CBD animals. Lastly we noticed that both sharp wave and ripple activity, after increasing upon transition to NREM, decreases as the bout last. This finding suggests that vehicle animals, who have longer bouts and less transitions, would display less sharp wave and ripple activity, although we found no significant difference in the amount of both brain events. This paradox suggests that there is still more to characterize in order to understand if CBD enhances or not memory consolidation. In sum, CBD changes anaesthesia-induced sleep by shortening the duration of both NREM and REM bouts, resulting in an increase in transitions between both state. As for sleep events, sharp waves appeared shorter among CBD animals, although the same difference was not observed for ripples. Finally, sharp wave and ripple activity appear to peak upon transition from REM to NREM sleep, and decreases as the NREM bout lasts longer, however, no effect of CBD on this observation was highlighted.

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Este trabalho apresenta um sistema de controle com o objetivo de atenuar as ondulações do torque eletromagnético considerando os harmônicos de quinta, sétima, décima primeira e décima terceira ordem da tensão do estator do Gerador de Indução Duplamente Alimentado (GIDA). Dessa maneira, propõe-se a utilização de controladores proporcional-integrativo vetoriais juntamente com um sistema de identificação dos componentes harmônicos da corrente do estator empregando a transformada discreta de Fourier. O trabalho aborda a influência dos harmônicos de quinta, sétima, décima primeira e décima terceira ordem da tensão do estator nos harmônicos da corrente do estator, nas ondulações do torque eletromagnético e nas potências ativa e reativa do estator. Resultados de simulação avaliam o controlador proposto em condições adversas: variações paramétricas e da frequência da rede, além da influência de atrasos na corrente do estator. Finalmente, resultados experimentais validam o sistema proposto. / This work presents a vector controller with the objective of reducing the electromagnetic torque and the active and reactive powers of the stator by means of control of the harmonics of the stator current in harmonic conditions of fifth, seventh, eleventh and thirteenth order of the stator voltage. The objective of the work is to propose a controller that uses two techniques already consolidated in the literature, proportional-integrative and discrete Fourier transform, besides being robust the main disadvantages of the existing harmonic controllers in the literature that are dependencies of the parameters of the machine and the Frequency of the network beyond the influence of the stator current delays on the harmonic controller. First, the influence of the fifth, seventh, eleventh, eleventh and thirteenth order harmonics of the stator voltage on the harmonics of the stator current and on the undulations of the electromagnetic torque and the active and reactive powers of the stator are studied. Next, simulation results evaluate the proposed controller under adverse conditions: parametric variations and the frequency of the network, besides the influence of stator current delays. Finally, experimental results validate the controller under conditions that the controller reference is nonzero.

Este trabalho apresenta um sistema de controle com o objetivo de atenuar as ondulações do torque eletromagnético considerando os harmônicos de quinta, sétima, décima primeira e décima terceira ordem da tensão do estator do Gerador de Indução Duplamente Alimentado (GIDA). Dessa maneira, propõe-se a utilização de controladores proporcional-integrativo vetoriais juntamente com um sistema de identificação dos componentes harmônicos da corrente do estator empregando a transformada discreta de Fourier. O trabalho aborda a influência dos harmônicos de quinta, sétima, décima primeira e décima terceira ordem da tensão do estator nos harmônicos da corrente do estator, nas ondulações do torque eletromagnético e nas potências ativa e reativa do estator. Resultados de simulação avaliam o controlador proposto em condições adversas: variações paramétricas e da frequência da rede, além da influência de atrasos na corrente do estator. Finalmente, resultados experimentais validam o sistema proposto. / This work presents a vector controller with the objective of reducing the electromagnetic torque and the active and reactive powers of the stator by means of control of the harmonics of the stator current in harmonic conditions of fifth, seventh, eleventh and thirteenth order of the stator voltage. The objective of the work is to propose a controller that uses two techniques already consolidated in the literature, proportional-integrative and discrete Fourier transform, besides being robust the main disadvantages of the existing harmonic controllers in the literature that are dependencies of the parameters of the machine and the Frequency of the network beyond the influence of the stator current delays on the harmonic controller. First, the influence of the fifth, seventh, eleventh, eleventh and thirteenth order harmonics of the stator voltage on the harmonics of the stator current and on the undulations of the electromagnetic torque and the active and reactive powers of the stator are studied. Next, simulation results evaluate the proposed controller under adverse conditions: parametric variations and the frequency of the network, besides the influence of stator current delays. Finally, experimental results validate the controller under conditions that the controller reference is nonzero.

Komplexe Muster neuronaler Aktivität entstehen während der Sharp-wave Ripples (SWRs) im Hippocampus und während der Up States im Neokortex (Zuständen mit hoher Aktivität). Sequenzen von Verhalten, die in der Vergangenheit erlebt wurden, werden während des komplexen Musters abgespielt. Die zugrunde liegenden Mechanismen sind nicht gründlich erforscht: Wie können kleine synaptische Veränderungen die großflächige Netzwerkaktivität während des Gedächtnisabrufes und der Gedächtniskonsolidierung kontrollieren? Im ersten Teil dieser Abhandlung wird die Hypothese aufgestellt, dass eine schwache synaptische Konnektivität zwischen Hebbschen Assemblies von der bereits vorhandenen rekurrenten Konnektivität gefördert wird. Diese Hypothese wird auf folgende Weise geprüft: die vorwärts gekoppelten Assembly-Sequenzen werden in neuronale Netzwerke eingebettet, mit einem Gleichgewicht zwischen exzitatorischer und inhibitorischer Aktivität. Simulationen und analytische Berechnungen haben gezeigt, dass rekurrente Verbindungen innerhalb der Assemblies zu einer schnelleren Signalverstärkung führen, was eine Reduktion der notwendigen Verbindungen zwischen den Assemblies zur Folge hat. Diese Aktivität kann entweder von kleinen sensorisch ähnlichen Inputs hervorgerufen werden oder entsteht spontan infolge von Aktivitätsschwankungen. Globale -- möglicherweise neuromodulatorische -- Änderungen der neuronalen Erregbarkeit können daher die Netzwerkzustände steuern, die Gedächnisabruf und die Konsolidierung begünstigen. Der zweite Teil der Arbeit geht der Herkunft der SWRs nach, die in vitro beobachtet wurden. Neueste Studien haben gezeigt, dass SWR-ähnliche Erscheinungen durch optogenetische Stimulation der Subpopulationen von inhibitorischen Neuronen hervorgerufen werden können (Schlingloff et al., 2014). Um diese Ergebnisse zu erklären wird ein de-inhibierendes Schaltkreis-Modell diskutiert, das die beobachteten Populationsausbrüche generieren kann. Die Auswirkungen der pharmakologischen GABAergischen Modulatoren auf die SWR-Häufigkeit werden in vitro untersucht. Die gewonnenen Ergebnisse wurden in Rahmen des Schaltkreis-Modells analysiert. Insbesondere wird den folgenden Fragen nachgegangen: Wie unterdrückt Gabazine, ein GABA_A-Rezeptor-Antagonist, die Entwicklung von SWRs? Wird das Zeitintervall zwischen SWRs durch die Dynamik der GABA_B Rezeptoren moduliert? / Complex patterns of neural activity appear during up-states in the neocortex and sharp-wave ripples (SWRs) in the hippocampus, including sequences that resemble those during prior behavioral experience. The mechanisms underlying this replay are not well understood. How can small synaptic footprints engraved by experience control large-scale network activity during memory retrieval and consolidation? In the first part of this thesis, I hypothesise that sparse and weak synaptic connectivity between Hebbian assemblies are boosted by pre-existing recurrent connectivity within them. To investigate this idea, sequences of assemblies connected in a feedforward manner are embedded in random neural networks with a balance of excitation and inhibition. Simulations and analytical calculations show that recurrent connections within assemblies allow for a fast amplification of signals that indeed reduces the required number of inter-assembly connections. Replay can be evoked by small sensory-like cues or emerge spontaneously by activity fluctuations. Global--potentially neuromodulatory--alterations of neuronal excitability can switch between network states that favor retrieval and consolidation. The second part of this thesis investigates the origin of the SWRs observed in in-vitro models. Recent studies have demonstrated that SWR-like events can be evoked after optogenetic stimulation of subpopulations of inhibitory neurons (Schlingloff et al., 2014; Kohus et al., 2016). To explain these results, a 3-population model is discussed as a hypothetical disinhibitory circuit that could generate the observed population bursts. The effects of pharmacological GABAergic modulators on the SWR incidence in vitro are analysed. The results are discussed in the light of the proposed disinhibitory circuit. In particular, how does gabazine, a GABA_A receptor antagonist, suppress the generation of SWRs? Another explored question is whether the slow dynamics of GABA_B receptors is modulating the time scale of the inter-event intervals.

[Truncated abstract] With rapidly increasing population densities along coastlines and rising global sea levels, coastal protection has become a major concern for coastal communities. Predicting sediment transport in nearshore regions, however, is one of the most challenging tasks faced by coastal researchers in designing coastal structures or beach nourishment schemes. Although nearshore sediment transport mainly occurs in the longshore direction, cross-shore sediment transport is crucial in determining the shoreline evolution and beach morphology . . . This study investigated the factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain through a series of field measurements conducted at several different locations and a numerical model. Only oscillatory flow components were examined and the mean flow components were not considered. Although many different factors such as cross-shore location with respect to breaker line, significant wave height to water depth ratio (Hs/h), normalised horizontal velocity skewness (<u³>/‹u²›³/²), median grain size (d50), breaker type, and wave groupiness appeared to influence the magnitude of cross-shore suspended sediment flux, bed ripples was identified as the major contributing factor in changing the direction of suspended sediment flux due to incident swell waves. Moreover, the direction changed significantly with ripple type. High frequency measurements, obtained to examine the influence of turbulent kinetic energy (TKE) on higher sediment suspension events observed under wave groups indicated that higher TKE was generated at the seabed by approaching wave groups, which in turn resulted in higher suspension events.°1

Au cours de cette thèse expérimentale et numérique nous avons étudié le comportement d’un empilement de grains dans deux situations différentes. Dans une première partie, une piste en sable est soumise au passage répété de véhicules, sous certaines conditions un motif régulier de rides apparaît spontanément : c’est l’instabilité de tôle ondulée. Ce phénomène, très répandu sur les routes en terre ou en gravier, est bien sûr très gênant pour les automobilistes mais est aussi très dangereux à cause des pertes d’adhérence qu’il provoque. Nous avons alors reproduit ce phénomène à l’échelle du laboratoire et également dans des simulations numériques de dynamique moléculaire de sphères molles. Grâce à ces outils expérimentaux et numériques nous avons pu mettre en évidence certaines propriétés de l’instabilité. Nous avons notamment mesuré la relation de dispersion des rides et mis en évidence que ce phénomène suit une instabilité de type fourche super-critique. En mesurant les contraintes agissant sur le véhicule nous avons alors pu construire une analyse de stabilité linéaire prédisant quantitativement le seuil de l’instabilité ainsi que la longueur d’onde du motif. Enfin, nous avons étudié des cas plus complexes où différents véhicules circulaient simultanément sur la piste. Nous avons également considéré des cas où la piste était humide et donc cohésive. Dans une seconde partie, nous avons étudié le comportement d’un silo rempli de grains soumis à des variations de température. On observe alors un lent fluage de la colonne que nous avons caractérisé. Deux régimes de fluage sont observés selon l’amplitude des cycles de température et nous avons obtenu une expression de la température critique séparant les deux régimes en fonction des paramètres microscopiques des grains (élasticité et rugosité de surface). Nous avons également étudié le mouvement individuel de chaque grain afin d’interpréter la dynamique macroscopique de l’empilement. / In this experimental and numerical thesis we have studied the behavior of a granular assembly in two different situations. In the first part, a sand track is subjected to repeated passages of vehicles, under certain conditions a regular pattern of ripples appears spontaneously: this is known as the washboard road instability. This phenomenon, very common on dirt roads, is of course annoying for drivers but is also very dangerous because of the lack of adhesion it causes. We reproduced this instability with a laboratory scale set-up and also thanks to soft spheres molecular dynamics simulations. With these experimental and numerical tools we have highlighted some properties of instability. In particular, we have measured the dispersion relation of the pattern and shown that this phenomenon follows pitchfork instability. By measuring the stress acting on the vehicle we were then able to build a linear stability analysis that predicts quantitatively the threshold of the instability and the wavelength of the pattern. Finally, we have studied more complex cases where several vehicles were simultaneously on the track. We have also considered the case where the track was wet and therefore cohesive. In a second part, we have studied the behavior of a silo filled with grains subjected to temperature variations. A slow creep motion of the grains in the column is observed. Two flow regimes are observed according to the amplitude of temperature cycles. We obtained an expression of the critical temperature between the two regimes as a function of the microscopic parameters of the grains (elasticity and surface roughness). We have also studied the motion of each individual grain in order to interpret the macroscopic dynamics of the pile.

La commande intermittente a pour but principal d'augmenter le rendement de l'ensemble machine-convertisseur par une réduction des pertes. Dans le but de maintenir le couple moyen de la machine requis par la charge, cette commande augmente le couple de référence et éteint quelques phases pendant chaque période électrique ou chaque période mécanique. Par ce fait, elle réduit les pertes du convertisseur et les pertes ferromagnétiques de la machine. Elle dispose également d'une liberté de sélection des phases activées qui influence des fréquences générées du courant, de la force radiale et du couple. Par conséquent, cette stratégie de la commande intermittente, qui est appelée le glissement, peut éviter l'excitation de fréquences naturelles du stator, ou bien celles de la chaine cinématique. Elle a pour but de réduire les effets négatifs de la commande intermittente vis-à-vis de comportements vibratoire du stator et pulsatoire de la chaine cinématique qui sont respectivement à l'origine du bruit acoustique et des à-coups mécaniques. En fin, la commande intermittente est validée sur des cycles de conduite et peut économiser jusqu’à 5,17 % de l’énergie électrique consommée. / The intermittent control aims to increase the efficiency of the machine-converter system by reducing the losses. In the purpose to maintain the average torque of the machine required by the load, this control increases the reference torque and turns off some phases during each electrical period or each mechanical period. By this fact, it reduces the converter losses and the ferromagnetic losses of the machine. It also has a freedom in the selection of the activated phases which influences the generated frequencies of the current, the radial force and the torque. Therefore, this strategy of the intermittent control, which is called sliding strategy, can avoid the excitation of the natural frequencies of the stator, or those of the drivetrain. It aims to reduce the negative effects of the intermittent control vis-à-vis the vibration of the stator and the pulsation of the drivetrain which are respectively at the origins of acoustic noise and mechanical jerks. Finally, the intermittent control is validated on driving cycles and can save up to 5.17 % of the consumed electrical energy.

Self-organization of regular surface pattern under ion beam erosion was described in detail by Navez in 1962. Several years later in 1986 Bradley and Harper (BH) published the first self-consistent theory on this phenomenon based on the competition of surface roughening described by Sigmund’s sputter theory and surface smoothing by Mullins-Herring diffusion. Many papers that followed BH theory introduced other processes responsible for the surface patterning e.g. viscous flow, redeposition, phase separation, preferential sputtering, etc. The present understanding is still not sufficient to specify the dominant driving forces responsible for self-organization. 3D atomistic simulations can improve the understanding by reproducing the pattern formation with the detailed microscopic description of the driving forces. 2D simulations published so far can contribute to this understanding only partially. A novel program package for 3D atomistic simulations called trider (TRansport of Ions in matter with DEfect Relaxation), which unifies full collision cascade simulation with atomistic relaxation processes, has been developed. The collision cascades are provided by simulations based on the Binary Collision Approximation, and the relaxation processes are simulated with the 3D lattice kinetic Monte-Carlo method. This allows, without any phenomenological model, a full 3D atomistic description on experimental spatiotemporal scales. Recently discussed new mechanisms of surface patterning like ballistic mass drift or the dependence of the local morphology on sputtering yield are inherently included in our atomistic approach. The atomistic 3D simulations do not depend so much on experimental assumptions like reported 2D simulations or continuum theories. The 3D computer experiments can even be considered as ’cleanest’ possible experiments for checking continuum theories. This work aims mainly at the methodology of a novel atomistic approach, showing that: (i) In general, sputtering is not the dominant driving force responsible for the ripple formation. Processes like bulk and surface defect kinetics dominate the surface morphology evolution. Only at grazing incidence the sputtering has been found to be a direct cause of the ripple formation. Bradley and Harper theory fails in explaining the ripple dynamics because it is based on the second-order-effect ‘sputtering’. However, taking into account the new mechanisms, a ‘Bradley-Harper equation’ with redefined parameters can be derived, which describes pattern formation satisfactorily. (ii) Kinetics of (bulk) defects has been revealed as the dominating driving force of pattern formation. Constantly created defects within the collision cascade, are responsible for local surface topography fluctuation and cause surface mass currents. The mass currents smooth the surface at normal and close to normal ion incidence angles, while ripples appear first at θ ≥ 40°. The evolution of bimetallic interfaces under ion irradiation is another application of trider described in this thesis. The collisional mixing is in competition with diffusion and phase separation. The irradiation with He+ ions is studied for two extreme cases of bimetals: (i) Irradiation of interfaces formed by immiscible elements, here Al and Pb. Ballistic interface mixing is accompanied by phase separation. Al and Pb nanoclusters show a self-ordering (banding) parallel to the interface. (ii) Irradiation of interfaces by intermetallics forming species, here Pt and Co. Well-ordered layers of phases of intermetallics appear in the sequence Pt/Pt3Co/PtCo/PtCo3/Co. The trider program package has been proven to be an appropriate technique providing a complete picture of mixing mechanisms.

This thesis reports on the controlled formation of surface rippling structures induced by tip scanning processes on compliant and brittle materials. Periodic surface structures were generated on polymeric and vitreous materials and with different length scales. Two aspects were focused on: the controlling of orientation and periodicity of the resulting structures via proper tuning the scan conditions and the physical mechanisms ruling the early stages of plowing wear causing the rippling effect. Specifically the influence of the scanned area geometric shape on the orientation of the rippling structure was investigated on a polystyrene surface. Nanoripples were induced by scanning the surface with a silicon tip using atomic force microscopy and dedicated scripts. Inside a structured area two ripple orientations can be observed: near boundaries the ripple orientation is determined by boundary orientation and regions away from the boundaries the ripples are aligned in a steady orientation. This steady orientation can be tuned by the distance between the scan lines. In the boundary regions the orientation of the ripples is different from steady orientation. The orientation of the boundaries clearly affected the orientation of the ripples and the tendency of the ripples to align in a steady angle defined by the scan parameters could be significantly modified. Geometric shapes like squares, circles, stars, pentagons and hearts allowed to distinguish the influence of curved and straight boundaries. Straight boundaries with different orientations allowed a detailed analysis of the influence of the angle on the rippling process. Straight boundaries inclined in the direction of the steady state angle of ripple orientation previously defined generate a uniform ripple pattern covering the entire scan area. The aspect of wear originating from the rippled surface was also investigated on similar polystyrene surfaces. As a result of repetitive scan passes spherical particles with diameters up to 250 nm were nucleated and detached from the surface. The particles originate from the crests of the ripples formed in the first scan pass. As proven by the lateral force signal the detachment occurs smoothly without a static friction peak suggesting a crazing mechanism induced by the scanning tip. Once detached from the surface the particles are displaced and piled up along the edges of scanned area. The formation of periodic surface structures was also investigated on a brittle silica glass. By a combination of scratch tests performed with a diamond microtip mounted in a nanoindenter and imaging with atomic force microscopy the existence of a periodic herringbone pattern inside scratch grooves on silica glass was proven. The rippled pattern was induced in the scratch process when the indenter was pulled laterally along the surface resulting in a microscopic scratch groove. The load was varied up to 30 nN and the scan velocity up to 500 µm/s. The resulting periodicity of the structures was found to increase linearly with increasing scratch velocity. The repetition distance was in the range of sub-µm and the corrugation in the range of a few hundred nm, which was well below indentation depth. In both cases, the surface rippling on a polymeric surfaces and the formation of a periodic pattern inside microscratches on a glass surface, the results were found to be consistent with minimalistic theoretical models for stick-slip.:Contents i Abstract iii Zusammenfassung v 1. Introduction 1 1.1. Periodic surface structures – relevance and formation 1 1.2. Surface rippling created by scanning probe lithography 2 1.3. Wear and nanoparticle release 4 1.4. Aim and outline 4 2. Experimental methods and fundamental concepts 6 2.1. Nanolithography 6 2.2. Atomic force microscopy 7 2.3. Nanoindentation and -scratching 10 2.4. Wear 11 2.5. Stick-slip motion 12 2.6. Spin coating 14 3. Surface rippling on polystyrene 15 3.1. Background and motivation 15 3.2. Methods 20 3.2.1. Sample preparation 20 3.2.2. Scanning probe lithography process 20 3.2.3. Imaging of structures and nanoparticles 21 3.3. Effect of boundaries on the orientation of surface rippling 22 3.4. Particle release as a result of surface rippling 31 4. Periodic structures inside scratches on silica glass 37 4.1. Background and motivation 37 4.2. Methods 38 4.2.1. Sample preparation 39 4.2.2. Scratch tests 39 4.2.3. AFM imaging and analysis 39 4.3. Surface rippling induces by scraping with a sharp indenter 40 5. Conclusion and outlook 49 A. Appendix surface rippling on polymers I B. Appendix surface rippling on glass IV Acknowledgements VII References IX