Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-21T19:38:27Z No. of bitstreams: 1 2016 - David Cabral Macedo.pdf: 619341 bytes, checksum: 189056ebf73c34fe51317abeb294ebc9 (MD5) / Made available in DSpace on 2018-09-21T19:38:27Z (GMT). No. of bitstreams: 1 2016 - David Cabral Macedo.pdf: 619341 bytes, checksum: 189056ebf73c34fe51317abeb294ebc9 (MD5) Previous issue date: 2016-03-01 / CAPES / The aim of this work was evaluate the effect of water deficit on two common bean (Phaseolus vulgaris L.) cultivars. For that, an experiment was carried out in greenhouse, with Ouro Negro and Diplomata cultivars, on Plant Science sector of Federal Rural University of Rio de Janeiro. The two cultivars were submitted to eight days of drought and four days of rehydration in a completely randomized design. The results show that the cultivar Diplomata has a better performance under water deficit conditions, presenting leaf base water potential values (?a) higher than the black gold in the last days of stress. Analysis of the fluorescence of chlorophyll a demonstrated statistically significant variation between cultivars, with the cultivar Diplomata presenting higher values for most of the parameters analyzed, such as Fv/Fm, ?F/F'm and lower values of the NPQ variable. About photoinhibition, the diplomat genotype showed a significant difference with lower values on the seventh day of stress and on the second day of rehydration, indicating a higher reversal of damage from photoinhibition throughout the day. Regarding the content of starch, the cultivar Ouro negro showed high values before stress (AE), but there was no significant difference among both cultivars during (DE) and in rehydration (R). In relation to the protein content, the cultivar Diplomata showed significantly higher values (AE) and in (R). Also, for the potential of biological nitrogen fixation (PFBN), the cultivar Diplomata showed a significantly higher number of nodules and nodules mass (AE) and (DE), as for the morphologic parameters, the Ouro negro showed higher root dry weight (MSR) at the (R), but Diplomata had a higher leaf dry weight (MSF) at the (R) too. For the yield components of the cultivar Diplomata had a significantly higher grain mass/plant under stress compared to the cultivar Ouro negro. Therefore, these results indicate a better adaptation to drought cultivar Diplomata. / Este trabalho teve como objetivo avaliar o efeito do d?ficit h?drico em dois cultivares de feijoeiro comum (Phaseolus vulgaris L.). Para isso, foi realizado um experimento em casa de vegeta??o, com os cultivares Ouro negro e Diplomata, no setor de Fitotecnia da Universidade Federal Rural do Rio de Janeiro. Os dois cultivares foram submetidos a oito dias de seca e quatro dias de reidrata??o em um delineamento inteiramente casualisado. Os resultados demonstram que o cultivar Diplomata tem uma melhor performance sob condi??es de d?ficit h?drico, apresentando valores de potencial de ?gua de base da folha (?a) maiores que o Ouro negro, nos ?ltimos dias de estresse. A an?lise da fluoresc?ncia da clorofila a demonstrou varia??o estatisticamente significativa entre os cultivares, com o cultivar Diplomata apresentando valores mais altos na maioria das vari?veis analisadas, como: o rendimento qu?ntico efetivo m?ximo do FS II (Fv/Fm), o rendimento qu?ntico efetivo do FS II (?F/F?m) e valores mais baixos no quenching n?o fotoqu?mico (NPQ). Sobre a fotoinibi??o, o gen?tipo Diplomata demonstrou diferen?a significativa com valores mais baixos no s?timo dia de estresse e no segundo dia de reidrata??o, indicando uma maior revers?o dos danos fotoinibit?rios no decorrer do dia. A respeito dos teores de amido, o cultivar Ouro negro apresentou valores significativamente mais altos antes do estresse (AE), mas n?o houve diferen?a significativa entre os gen?tipos, durante o estresse (DE) e na reidrata??o (R). J? em rela??o aos teores de prote?nas sol?veis em folhas, o cultivar Diplomata demonstrou manter valores significativamente maiores (AE) e (R). Em rela??o ?s vari?veis de potencial de fixa??o biol?gica de nitrog?nio (PFBN), o cultivar Diplomata tamb?m apresentou um maior n?mero de n?dulos e maior massa de n?dulos (AE) e (DE), assim como nas vari?veis morfol?gicas, apesar do cultivar Ouro negro apresentar uma maior massa seca de raiz (MSR) (DE) e na (R), o cultivar Diplomata apresentou uma maior massa seca foliar (MSF) na (R). Quanto aos componentes de produ??o, o cultivar Diplomata apresentou uma maior massa de gr?os/planta, sob estresse, quando comparado com o cultivar Ouro negro. Esses resultados indicam uma maior adapta??o ? seca do cultivar Diplomata quando comparado ao Ouro negro.

Orientador: Danilo Domingues Millen / Resumo: O objetivo deste estudo foi avaliar a classificação das baias com base no consumo logo nas semanas iniciais do confinamento de machos castrados e novilhas, sobre o desempenho, e desenvolver modelos de predição da ingestão de matéria seca (IMS), procurando melhorar sua acurácia por meio da inclusão da fase de confinamento em que os animais se encontram. Isso possibilitaria a identificação de “baias problemas”, ou de desempenho superior, facilitando a tomada de decisão na gestão. Para isso foram utilizados dados de 3.650 baias de confinamentos comerciais dos USA (2.256 de machos castrados e 1.394 de novilhas), os quais foram confinados entre os anos de 2009 a 2014. Os dados de IMS e de energia líquida de ganho (ELg) médios do período de terminação foram ajustados de acordo com o peso vivo inicial e dias em alimentação (DEA) pelo PROC NLIN do SAS (2009) separadamente para machos castrados e novilhas. Tanto a IMS como a ingestão de ELg médias ajustadas do período de terminação foram correlacionados com a ingestão das semanas de 5 a 12 pelo PROC CORR, e de acordo com a semana de maior correlação as baias foram classificadas em quartis: Superior ¼, Alta ¼, Média ¼ e Baixa ¼ do maior para o menor consumo e avaliado o desempenho pelo PROC MIXED. Para o desenvolvimento dos modelos foi utilizado o PROC CORR para identificar a correlação de cada variável (PVI, IMS de 8 a 28 dias, sexo, IMS da semana anterior) com a IMS e ordenar a inclusão delas no modelo, e por meio do PROC MIXED foi t... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

E. coli SecA is an essential component for protein translocaiton across membrane. SecA can be deleted from its N- and/or C-terminal ends without losing complementation activity. In this study, we determined the dispensity of both ends of SecA molecule. The minimal length at the SecA C-terminus is dependent on the length of the N-terminal region. SecA10-826 and SecA22-829 are the two minimal length SecAs. One more amino acid deleted at the C-terminal end completely abolished their complementation activity. A hydrophobic amino acid is required at the 826th amino acid in the minimal-length SecAs. Both SecA22-828 and SecA22-829 could form a dimer, and have decreased ATPase and protein translocation activities. The active truncated SecA mutants tended to have more soluble form than membrane-bound form, but were stably embedded in membrane. In contrast, the inactive truncated SecA mutants tended to have more membrane-bound form, but were not stable in membrane. Thus, the loss of complementation is not related to dimerization, ATPase and translocation activity but to certain extent related to their biased subcelluar localization and conformation in membrane. Isolated membranes of E coli strains were solubilized and fractionated by sucrose gradient fractionation. These membranes fractions were depleted of SecY and YidC, but contained SecD, SecF and GroEL. Proteoliposomes reconstituted from these fractionated membrane proteins were active in pOmpA translocation which required SecA and ATP. Membrane fractions from strain CK1801 in which the unc gene is deleted were reconstituted into liposomes and also showed translocation activities. Moreover, proteoliposomes reconstituted with Bacteriorodopsin alone were not active in translocation, while proteoliposomes reconstituted with Bacteriorodopsin and CK1801 membrane fractions showed elevated translocation efficiency. These data suggested that proton motive force is not obligatory for, but stimulatory to translocation of pOmA. Purified GroEL was reconstituted into lipsomes and the reconstituted proteoliposomes were active in pOmpA translocation although at lower efficiency. This translocation also required SecA and ATP. These results together suggested that translocation of pOmpA is active in the absence of SecY and YidC. In the absence of SecYEG, translocation of pOmpA requires SecA and ATP. GroEL, SecD and SecF may participate in the SecY-independent translocation.

SecA is a central component of the general secretion system that is essential for growth and virulence of bacteria. A series of fluorescein analogs were tested against ATPase activities of Escherichia coli SecA. Rose Bengal (RB) and Erythrosin B are potent inhibitors abolishing the activities of three forms of SecA ATPase with IC50 in µM range. Both inhibit SecA intrinsic ATPase with two mechanisms depending on ATP concentrations, indicating they influence the two non-identical nucleotide binding sites differently. RB shows different inhibitory effects against three forms of SecA ATPase activities, suggesting that the inhibition is related to the conformation of SecA. RB with IC50 at sub-µM level is the most potent inhibitor of SecA ATPases and SecA-dependent protein translocation to date. The fluorescein analogs inhibit intrinsic ATPase of Bacillus subtilis SecA similarly, and also exhibit antibacterial effects in E. coli and B. subtilis. Our findings indicate the value of fluorescein analogs as probes for mechanistic studies of SecA and the potential development of new SecA-targeted antimicrobial agents. A series of SecA derivatives with truncated C-terminus within the first long α-helix of the helix-bundle extending the ATPase catalytic domain of N68 was analyzed. These SecA variants interact with lipids, and those containing the C-terminal portion of the long α-helix starting at residues #639 form the ring-like structure in liposomes, indicating the critical domains for forming the protein-conducting channel. The presence and length of the C-domain influence the response to RB of NBDII mutants and C-terminal truncates of SecA. Thus this region may interact with the inhibitors and is involved in the structure and regulation of SecA ATPase activity. B. subtilis SecA was analyzed for interspecies comparison. Despite sharing high homology, this SecA homolog cannot complement E. coli mutants with SecA defect. Phospholipids do not stimulate ATPase activities of B. subtilis SecA, but induce its conformational changes, leading to the lipid-specific domains and ring-like structures similar to E. coli SecA. These pore-ring structures may represent part of the protein-conducting channels. Therefore, the potential structural roles of SecA in the protein translocation machinery may be universal in both Gram-negative and Gram-positive bacteria.

Protein translocation is essential for bacterial survival and the most important translocation mechanism in bacteria is the secretion (Sec) pathway. Thus targeting Sec pathway is a promising strategy for developing novel antibacterial therapeutics. We report the design, syntheses, mechanistic studies and structure-activity relationship studies using HQSAR and 3-D QSAR Topomer CoMFA analyses of 4-oxo-5-cyano thiouracil derivatives. In summary, introduction of polar group such as –N3 and linker groups such as –CH2-O- enhanced the potency as well as logP and logS several fold. We also report the discovery, optimization and structure-activity relationship study of 1,2,4-triazole containing pyrimidines as novel, highly potent antimicrobial agents. A number of inhibitors have been found to inhibit microbial growth at high nanomolar concentrations.

Protein translocation is essential for bacterial survival and the most important translocation mechanism in bacteria is the secretion (Sec) pathway. Thus targeting Sec pathway is a promising strategy for developing novel antibacterial therapeutics. We report the design, syntheses, mechanistic studies and structure-activity relationship studies using HQSAR and 3-D QSAR Topomer CoMFA analyses of 4-oxo-5-cyano thiouracil derivatives. In summary, introduction of polar group such as –N3 and linker groups such as –CH2-O- enhanced the potency as well as logP and logS several fold. We also report the discovery, optimization and structure-activity relationship study of 1,2,4-triazole containing pyrimidines as novel, highly potent antimicrobial agents. A number of inhibitors have been found to inhibit microbial growth at high nanomolar concentrations.

A Ceratoconjuntivite Seca (KCS Keratoconjunctivitis Sicca) é uma desordem imunomediada e resulta de alterações do componente aquoso do filme lacrimal e da deficiência dos componentes lipídicos e mucoso.Seu diagnóstico é baseado no Teste Lacrimal de Schirmer (TLS) e no Teste de Ruptura do Filme Lacrimal (TRFL) e tem como sinais clínicos: secreção mucopurulenta, hiperemia conjuntival, blefaroespasmos, fotofobia, incômodo, dor, vascularização, opacidade corneana e pigmentação, além de cegueira em casos avançados. O tratamento convencional consiste em aplicações diárias de Ciclosporina 0,2% ou Tacrolimus 0,03% (pomada ou colírio oftálmicos), que apesar de controlar a doença, são custosos, não curativos e exigem alto comprometimento da interação paciente-proprietário. A terapia celular usando células-tronco (CT) traz uma nova esperança para doenças sem tratamento efetivo. Neste trabalho utilizamos CT mesenquimais (CTM) obtidas a partir de membrana amniótica (CTMA) de cães obtidas a partir do descarte destes tecidos em campanhas de castrações em diferentes tempos gestacionais, sem formação tumoral quando submetidas ao teste tumorigênico durante 60 dias. Dois animais com KCS crônica foram tratados com duas injeções de CTMA com intervalo de 30 dias, sendo a primeira de 0,5x106 células e a segunda de 1x106 células em cada glândula. Na segunda semana após a terapia foi observado aumento da TLS sugerindo um benéficio da terapia que foi diminuindo com o passar das semanas. O TRFL oscilou durante os testes e não apresentou diferenças significativas. A terapia celular utilizando CTMA de cães melhorou a condição ocular nos dois casos em momentos e parâmetros variados, com repercussão na melhoria da superfície, mas não houve regressão do quadro clínico. Investigações futuras em estágios menos avançados da doença podem ajudar a elucidar os mecanismos pelos quais esse efeito foi obtido / Keratoconjunctivitis Sicca (KCS) is an immune-mediated disorder and results from changes in the aqueous component of the tear film and also from the impairment of lipid and mucous components. Diagnosis is based on Lacrimal Schirmer Test (LST) and Tear Film Break Test (TFBT) and its clinical signs: purulent discharge, conjunctival hyperemia, blefaroespasmos, photophobia, discomfort, pain, vascularization, corneal opacity and pigmentation, addition of blindness in advanced cases. The standard treatment consists of daily applications of 0.2% cyclosporin or tacrolimus 0.03% (ophthalmic ointment or drops), that in spite of controlling the disease are expensive, require the patient does not curative and and require greater involvement of the patient-owner interaction. Cell therapy using stem cells (SC) brings new hope for diseases without effective treatment. In this work we used CT mesenchymal cells (MSCs) obtained from dogs amniotic membrane (AMSC) in the capanhas of castration at different times gestacionai without tumor formation tumorigenic when subjected to the test during 60 days. Two animals with chronic KCS were treated with two injections of CTMA with 30-day intervals, the first of which 0,5x106 cells and the second of 1x106 cells in each gland. In the second week after therapy was an increase in LST that has been decreasing over the weeks. The BTTF fluctuated during testing and no significant differences. Cell therapy using AMSC dogs improved the eye condition in both cases at times and different parameters, with consequent improvement in ocular surface but there was no regression of the clinical picture. Future research in less advanced stages of the disease may help to elucidate the mechanisms by which this effect was obtained

A seca é um dos principais problemas que afetam a produção do feijoeiro. A despeito da importância de caracteres fenotípicos radiculares, muitos dos esforços de melhoramento genético da cultura tem focado na seleção de cultivares com maior produção de grãos. A simbiose estabelecida entre plantas e FMA aumentam o potencial de captação de água no solo através das extensas redes formadas pelas hifas e alteram vias metabólicas vitais para a manutenção das relações hídricas da planta. O modelo de interação feijoeiro (BAT 477) colonizado por uma mistura de FMA (Glomus clarum, Acaulospora scrobiculata e Gigaspora rosea) foi submetido a um déficit hídrico de 96 h durante o pré-florescimento. O transcritoma global de raízes inoculadas e não-inoculadas, sujeitas ou não à seca, foi comparado por RNA-Seq. Um conjunto de 71 transcritos foram induzidos por FMA durante a seca. Comparando-se os tratamentos estresse e controle, 12.086 unigenes foram regulados em plantas inoculadas e 11.938 em não-inoculadas, refletindo o alto potencial de tolerância da linhagem BAT 477 e indicando que a presença de FMA produz uma regulação fina no perfil de expressão de genes regularmente envolvidos na resposta da planta ao estresse. Foram selecionados 15 fatores de transcrição e seus perfis de expressão foram caracterizados por RT-qPCR tomando-se três períodos, 48, 72 e 96 h de déficit hídrico. Plantas inoculadas ativaram a expressão destes genes mais tardiamente (após 72 h), refletindo melhorias nas condições hídricas da planta que adiam a percepção do estresse. Adicionalmente, a expressão de 23 transcritos foi avaliada em três amostras teciduais diferentes obtidas por microscopia de microdissecção a laser. Glucan 1,3 ?-Glucosidase e PIP2,3, foram detectados somente em células do córtex radicular contendo arbúsculos indicando uma possível indução tecido específica dependente da presença dos fungos. Análises complementares apontaram a regulação de 171 unigenes envolvidos na resposta das FMA ao estresse. Estes resultados validam a hipótese inicial de que a inoculação com FMA altera os perfis de expressão de genes vitais para a resposta da planta ao déficit hídrico / Drought is one of the main problems that affect common bean\'s production. Despite the importance of root fenological characters, breeding efforts for the culture have focused on the selection of cultivars for grain yield. The symbiosis stablished between AMF and plants enhances the potential of water absorption from the soil through an extensive net formed by hyphae and alters vital metabolic pathways involved in the maintenance of the water relations in plants. The interaction model common bean (BAT 477) colonized by a mixture of AMF (Glomus clarum, Acaulospora scrobiculata and Gigaspora rosea) was exposed to a water deficit regime of 96 h during pre-flowering. Global transcriptome from inoculated and non-inoculated roots, exposed or not to drought, were compared through RNA-Seq. A set of 71 transcripts was induced by AMF during drought. Comparing both stress and control treatments, 12,086 unigenes were regulated in inoculated plants, and 11,938 in non-inoculated, reflecting the great tolerance potencial of the lineage BAT 477 and indicating that the presence of AMF produces a fine tune regulation on the expression of genes regularly involved on the drought response of the plant. It was selected 15 transcription factors and their expression profiles were characterized through RT-qPCR taking 3 periods, 48, 72 and 96 h of water deficit. AM plants activated earlier (after 72 h) the expression of these genes, reflecting improvements on the water conditions of the plant that delay the stress perception. Additionally, the expression of 23 transcripts was evaluated on three different tissue samples obtained through laser microdissection microscopy. Glucan 1,3 ?-Glucosidase and PIP2,3, were detected only in cortical cells containing arbuscules, pointing to a possible tissue specific induction dependent of the presence of the fungus. Additional analysis point to the regulation of 171 unigenes involved on the response of the AMF to drought. These results corroborate the initial hypothesis that the inoculation with AMF alters the gene expression profiles of genes that are vital for water deficit response in plants

Made available in DSpace on 2014-06-11T19:30:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T18:40:34Z : No. of bitstreams: 1 machado_raf_dr_botfca.pdf: 1268669 bytes, checksum: a94002e1be9b4d17ac8aabc97d374c98 (MD5) / O presente trabalho teve por objetivo avaliar parâmetros fisiológicos e acúmulo diferencial de matéria seca e nutrientes em linhagens de milho contrastantes para tolerância à seca e eficiência de utilização do nitrogênio. Foram desenvolvidos 2 experimentos em casa de vegetação da Embrapa Milho e Sorgo, Sete Lagoas – MG. No primeiro se foram avaliadas 4 linhagens de milho, 2 tolerantes (L6.1.1 e L13.1.2) e 2 sensíveis à seca (L1147 e L1170) submetidas a 2 regimes hídricos (controle e estresse) e fases da cultura (estádio V6, florescimento e ambas). O estresse no estádio V6 não influenciou nas características avaliadas, porém o défice hídrico durante o florescimento resultou em retardamento no florescimento das linhagens sensíveis com elevada resistência estomática e potencial hídrico resultando em significativa redução na transpiração. A produção de matéria seca das linhagens foi influenciada pelo défice hídrico. A produção de espigas e grãos das linhagens tolerantes foi muito superior às linhagens sensíveis. No segundo experimento as linhagens L13.1.2 e L1170 foram submetidas a 2 regimes hídricos (controle e estresse) e duas doses de nitrogênio (20 e 80 mg dm-3 solo) durante o florescimento. O défice hídrico e a menor dose de nitrogênio aumentaram o intervalo entre os florescimentos masculino e feminino. As linhagens exibiram redução na resistência estomática quando se aplicaram 80 mg N dm-3 solo. A adição de maior dose de N propiciou acréscimos significativos na matéria seca das folhas e espigas, enquanto o défice hídrico reduziu a matéria seca do colmo. A adição de N promoveu aumentos nos teores de clorofila das folhas. O défice hídrico não influenciou a L1170, enquanto que a L13.1.2 apresentou maiores teores relativos de clorofila sob défice hídrico. Na análise da fluorescência da clorofila não foram detectadas diferenças... / The study had for objectives to evaluate physiological parameters and accumulation of dry matter and nutrients in contrasting inbred lines for drought tolerance and nitrogen efficiency use. Two experiments were carried out greenhouse of Embrapa Maize and Sorghum, Sete Lagoas, MG – Brazil. In the first were evaluated two tolerant corns (L6.1.1 and L13.1.2) and two sensitive to drought (L1147 and L1170) submitted to two water regimes (control and stress) and of crop growth (V6 stage, flowering and both). The water stress at V6 stage didn’t have influence in the characteristics evaluated. The water stress at flowering resulted in delayed flowering, high stomatic resistance and water potential with significant reduction in evapotranspiration in sensitive corns. The dry matter production of inbred lines was influenced by the water stress. The ears and grains production of tolerant corn were greater than sensitive. In the second experiment, inbred lines L13.1.2 and L1170 were submitted to two water regimes (control and stress) and two level of nitrogen (20 and 80 mg N dm-3 soil) during flowering. The water deficit and the low level of N increased the anthesis silking interval. The application of 80 mg N dm-3 soil resulted in reduction in the stomatic resistance of inbred lines. The addition of high level of N increased the dry matter of the leaves and ears, while the water deficit reduced the dry matter of stem. The addition of N promoted increases in chlorophyll content of leaves. The water deficit didn’t have effect in L1170, however L13.1.2 presented higher chlorophyll relative content under water stress. In chlorophyll fluorescence analyzes were not observed differences in the initial fluorescence...(Complete abstract, click electronic access below)

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-08-16T20:39:15Z No. of bitstreams: 1 SilvanaSantanaGomes_DISSERT.pdf: 1210897 bytes, checksum: 94537b2894ce19414f5eb0385809548e (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-18T23:45:27Z (GMT) No. of bitstreams: 1 SilvanaSantanaGomes_DISSERT.pdf: 1210897 bytes, checksum: 94537b2894ce19414f5eb0385809548e (MD5) / Made available in DSpace on 2016-08-18T23:45:27Z (GMT). No. of bitstreams: 1 SilvanaSantanaGomes_DISSERT.pdf: 1210897 bytes, checksum: 94537b2894ce19414f5eb0385809548e (MD5) Previous issue date: 2016-03-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O processo de eutrofiza??o tem como principal consequ?ncia o aumento da biomassa algal, principalmente flora??es de cianobact?rias. As elevadas taxas de evapora??o associadas ?s reduzidas taxas de precipita??o, caracter?sticas das regi?es semi?ridas, contribuem para o aumento da disponibilidade de nutrientes em per?odos de seca e consequente agravamento da condi??o eutr?fica nos mananciais. As mudan?as clim?ticas tendem a intensificar os sintomas de eutrofiza??o, principalmente na regi?o semi?rida. Diante disso, a hip?tese do trabalho ? que a redu??o do volume, causada pela seca prolongada, aumenta a biomassa algal devido ao aumento da disponibilidade de recursos (nutrientes). Logo, o objetivo do estudo ? avaliar o impacto da seca prolongada na biomassa algal em um manancial mesotr?fico da regi?o semi?rida tropical. O decr?scimo do volume esteve associado ? degrada??o da qualidade da ?gua, com aumento nas concentra??es de nutrientes e redu??o da transpar?ncia da ?gua. A maior disponibilidade de nutrientes na coluna d??gua, consequ?ncia da seca, forneceu os recursos necess?rios para o crescimento algal e permitiu a altera??o do estado tr?fico do reservat?rio Boqueir?o de mesotr?fico para eutr?fico. O presente estudo demonstrou que o prolongamento da seca degrada a qualidade da ?gua. O efeito da seca em Boqueir?o foi tardio devido ? baixa disponibilidade inicial de nutrientes nesse reservat?rio. A redu??o do volume, proporcionada pelo balan?o h?drico negativo caracter?stico do semi?rido e intensificada pelo evento de seca prolongada, foi capaz de aumentar a disponibilidade de nutrientes e provocar o aumento da biomassa algal e consequente altera??o do estado tr?fico do manancial. / The main consequence of eutrophication is an increase in algal biomass, mainly cyanobacterial blooms. The high evaporation and low precipitation, characteristics of semiarid regions, contribute to the nutrients availability increase in drought periods and consequent aggravation of eutrophic condition in reservoirs. Climate changes tend to intensify eutrophication symptoms, mostly in a semiarid region. Therefore, the aim of this study was to evaluate the impact of an extended drought in algal biomass in Parelhas?s Boqueir?o, a mesotrophic reservoir located in a semiarid tropical region. The low volume was associated to water quality degradation and to the high nutrients concentrations and low water transparency. The increase in nutrients availability in the water column, consequence of reduced precipitation and low reservoir?s volume, provided the necessary resources for algal growth and allowed a change in trophic state in Boqueir?o reservoir. This study showed how an extended drought decreases water quality. The effect of drought in Boqueir?o was late detected due to the reservoir?s low initial nutrients concentration. The reservoir?s volume reduction increased the nutrient availability along with the algal biomass increase and the reservoir?s trophic state change of mesotrophic to eutrophic.