Global ETD Search

141	A unique serpin P1′ glutamate and a conserved β-sheet C arginine are key residues for activity, protease recognition and stability of serpinA12 (vaspin) Ulbricht, David, Pippel, Jan, Schultz, Stephan, Meier, René, Sträter, Norbert, Heiker, John T. 06 March 2019 (has links) SerpinA12 (vaspin) is thought to be mainly expressed in adipose tissue and has multiple beneficial effects on metabolic, inflammatory and atherogenic processes related to obesity. KLK7 (kallikrein 7) is the only known protease target of vaspin to date and is inhibited with a moderate inhibition rate. In the crystal structure, the cleavage site (P1-P1′) of the vaspin reactive centre loop is fairly rigid compared with the flexible residues before P2, possibly supported by an ionic interaction of P1′ glutamate (Glu379) with an arginine residue (Arg302) of the β-sheet C. A P1′ glutamate seems highly unusual and unfavourable for the protease KLK7. We characterized vaspin mutants to investigate the roles of these two residues in protease inhibition and recognition by vaspin. Reactive centre loop mutations changing the P1′ residue or altering the reactive centre loop conformation significantly increased inhibition parameters, whereas removal of the positive charge within β-sheet C impeded the serpin–protease interaction. Arg302 is a crucial contact to enable vaspin recognition by KLK7 and it supports moderate inhibition of the serpin despite the presence of the detrimental P1′ Glu379, which clearly represents a major limiting factor for vaspin-inhibitory activity. We also show that the vaspin-inhibition rate for KLK7 can be modestly increased by heparin and demonstrate that vaspin is a heparin-binding serpin. Noteworthily, we observed vaspin as a remarkably thermostable serpin and found that Glu379 and Arg302 influence heat-induced polymerization. These structural and functional results reveal the mechanistic basis of how reactive centre loop sequence and exosite interaction in vaspin enable KLK7 recognition and regulate protease inhibition as well as stability of this adipose tissue-derived serpin. info:eu-repo/classification/ddc/572 ddc:572
142	IMPLEMENTING NETCONF AND YANG ON CUSTOM EMBEDDED SYSTEMS Georges, Krister, Jahnstedt, Per January 2023 (has links) Simple Network Management Protocol (SNMP) has been the traditional approach for configuring and monitoring network devices, but its limitations in security and automation have driven the exploration of alternative solutions. The Network Configuration Protocol (NETCONF) and Yet Another Next Generation (YANG) data modeling language significantly improve security and automation capabilities. This thesis aims to investigate the feasibility of implementing a NETCONF server on the Anybus CompactCom (ABCC) Industrial Internet of Things (IIoT) Security module, an embedded device with limited processing power and memory, running on a custom operating system, and using open source projects with MbedTLS as the cryptographic primitive library. The project will assess implementing a YANG model to describe the ABCC’s configurable interface, connecting with a NETCONF client to exchange capabilities, monitoring specific attributes or interfaces on the device, and invoking remote procedure call (RPC) commands to configure the ABCC settings. The goal is to provide a proof of concept and contribute to the growing trend of adopting NETCONF and YANG in the industry, particularly for the Industrial Internet of Things (IIoT) platform of Hardware Meets Software (HMS). NETCONF MbedTLS YANG Embedded SNMP XML HMS IETF IIoT ABCC Wireshark TLS Porting RTOS IoT Interoperability Networking Security Protocols C/C++ Embedded Linux Configuration Management Microcontroller Microprocessor Scalability Hardware Interfaces Hardware Open source QA Data Modeling System Integration Automation Computer Sciences Datavetenskap (datalogi) Software Engineering Programvaruteknik Embedded Systems Inbäddad systemteknik Telecommunications Telekommunikation Communication Systems Kommunikationssystem Computer Systems Datorsystem
143	Odposlech moderních šifrovaných protokolů / Interception of Modern Encrypted Protocols Marček, Ján January 2012 (has links) This thesis deals with the introduction to the security mechanism.The procedure explains the basic concepts, principles of cryptography and security of modern protocols and basic principles that are used for information transmission network. The work also describes the most common types of attacks targeting the eavesdropping of communication. The result is a design of the eavesdropping and the implementation of an attack on the secure communication of the SSL protocol..The attacker uses a false certificate and attacks based on poisoning the ARP and DNS tables for this purpose. The thesis discusses the principles of the SSL protocol and methodology of attacks on the ARP and DNS tables.

Page generated in 0.0359 seconds