La conductivité hydraulique foliaire (KF) est une composante majeure du transport d’eau dans toute la plante. Dans les feuilles de noyer, la KF est stimulée à la lumière et est étroitement liée à l’accroissement du taux des transcrits d’aquaporines JrPIP2s. Par ailleurs, la corrélation entre la stimulation de la KF et des transcrits d’aquaporines à la lumière, n’est pas générale et dépend de l’espèce. Ici, nous étudions cette corrélation chez cinq espèces forestières (Juglans regia, Fagus sylvitica, Quercus robur, Salix alba et Populus tremula) différant par leur réponse à la lumière. Nous démontrons seulement chez le noyer (Juglans regia), la contribution des deux familles d’aquaporines PIP1s et PIP2s. Afin de mieux comprendre le rôle des JrPIP1s et JrPIP2 dans la réponse à la lumière, nous avons isolé 8 nouvelles isoformes dans les feuilles de noyer et nous avons étudié leurs profils d’expression sur une cinétique lumière. Toutes les isoformes étudiées sont accumulées à la lumière et réprimées à l’obscurité. De plus, la KF est dépendante de la qualité de lumière. Elle est réduite de 65% en absence de lumière bleue. Cette diminution serait liée à l’inhibition des transcrits d’aquaporines. Afin de caractériser les mécanismes moléculaires précoces impliqués dans la modulation de KF par la lumière, l’approche globale cDNA-AFLP a été menée sur des feuilles de noyer sous différentes conditions d’éclairement. Nous obtenons 12000 transcrits différentiels dérivés (TDFs) générés par les 128 couples d’amorces. Parmi les 187 séquences obtenues, 93 d’entre elles ont une fonction putative. Leur classification fonctionnelle montre que les gènes relatifs à la régulation cellulaire représentent environ 58% des TDFs identifiés. Les feuilles exposées à la lumière, montrent des changements dans les voies de : signalisation calcique, protéolyse, trafic vésiculaire et l’expression de divers facteurs de transcription et protéines de régulation. Pour mieux comprendre le rôle potentiel de la signalisation calcique dans la modulation de la KF par la lumière, nous avons étudié l’effet d’un inhibiteur des canaux calciques [LaCl3] et d’un antagoniste de calmoduline [W7] sur la KF et les transcrits des 10 JrPIPs. Comparées aux feuilles témoins, les inhibiteurs calciques provoquent une réduction de la KF et de la majorité des JrPIPs étudiées à la lumière. Nos résultats confirment l’implication du complexe Ca2+ /calmoduline dans la transduction du signal lumineux responsable de la stimulation de la KF et des transcrits d’aquaporines chez le noyer. / Leaf hydraulic conductance (Kleaf) takes a significant part in plant water relations. In walnut leaves, Kleaf was stimulated by light and tightly related to accumulation of JrPIP2s aquaporin transcripts. However, the light effect on Kleaf value is not systematically related to aquaporin regulation. Here we investigated the relationship between light, Kleaf and transcript levels of aquaporin in five species (Juglans regia, Fagus sylvitica, Quercus robur, Salix alba and Populus tremula) differing by the response of their Kleaf to light. Only for walnut leaves, we showed that light-increased Kleaf value is closely related to higher stimulation of both PIP1s and PIP2s aquaporins. To further investigate the involvement of aquporins (JrPIP1s and JrPIP2) in the light Kleaf modulation, 8 new full length aquaporins have been identified in walnut leaves and their expression pattern was monitored. All the aquaporin tested was turned up to be upregulated under light condition and downregulated under darkness. Moreover, we showed that the Kleaf response to light is quality-dependant, since it was reduced of 65% in the absence of blue light. Interstingly, this Kleaf reduction was correlated with a high downregulation of almost all aquaporins tested. To give an insight into the early molecular events involved in the light-induced Kleaf regulation, a large-scale transcriptomic analysis consisting of the cDNA-AFLP procedure was carried out on walnut leaves, kept at different light conditions. We obtained a total of 12,000 transcript-derived fragments (TDFs) by cDNA-AFLP with 128 primer pairs. Reliable sequences were obtained for 187 of these TDFs, and functions were attributed to 93 TDFs through BLAST searches in GenBank databases. Most of the 93 TDFs corresponded to genes encoding proteins involved in cellular regulation (58%). Leaves exposed to light showed changes in the Ca2+-signaling pathway, the ubiquitin-proteasome pathway, vesicle trafficking process and the expression of multiple transcription factors and protein regulators. To progress in understanding of a potential role for calcium signalling in light-modulated Kleaf, Kleaf values and transcript accumulation of 10 JrPIPs were monitored in leaves perfused with either a blocker of calcium channels [LaCl3] or a CaM antagonist [W7]. Compared to control, these Ca2+ -effectors led to a significant reduction in Kleaf and transcripts accumulation of almost all JrPIPs under light conditions. Our results indicate that Ca2+ /calmodulin complex may transduce the light signal required for stimulation of Kleaf and its correlated aquaporin expression.

<p>Thesis submission for Caleb Kell</p>

This paper investigates chemical bleaching of wood and its ageing to make specie specific recommendations on which bleaching solutions to use when color adjusting veneer for furniture restoration.  In more detail, chemical bleaching of European walnut (Juglans regia), Rio rosewood (Dalbergia nigra), purpleheart (Peltogyne spp.), Honduras mahogany (Swietenia macrophylla) and padauk (Pterocarpus spp.) has been investigated using eleven different bleaching solutions. Both oxidative (e.g. hydrogen peroxide and potassium permanganate) and reductive (e.g. oxalic acid and sodium bisulfite) solutions have been used. Furthermore, to investigate aging of the bleached surfaces they were subjected to sunlight behind a glass window until a change in Blue Wool Scale 3 was obtained. Visual examination has been used to rank the color change after the chemical bleaching and the sun light exposure experiment. A small color change after the sun light exposure experiment indicates a high degree of lightfastness and is preferred for long term stability when restoring furniture. The results show that the wood species react in different ways to the investigated bleach solutions and to sun light exposure. It can also be noted that all investigated solutions were not suitable for all wood species. Moreover, the sun light exposure experiment identified effective bleach solutions that gave an unacceptable low lightfastness for mahogany and padauk after chemical bleaching. Based on the chemical bleaching and sun light exposure results, preferred bleaching solutions are recommended for the investigated wood species. / En vanlig restaureringsuppgift är att ställvis ersätta borttappat träfaner på en möbel. Originalfaneret har blekts med tiden, varför det nya faneret ofta är för färgstarkt och färgen behöver reduceras. När vi upplever en bit padouk som intensivt röd eller amarant som lila, beror det på att det i trät finns ämnen som tar upp energi motsvarande energin i våglängder av synligt ljus. När ämnena absorberar en del av det synliga ljuset registrerar våra näthinnor att de saknas, vilket tolkas som en färg av vår hjärna. De ämnen som absorberar synligt ljus kan brytas ner av solljus över längre tid eller på några minuter genom tillförseln av ett oxidations- eller reduktionsmedel. I mitt examensarbete undersöker jag hur olika oxidations- och reduktionsmedel bleker olika träslag. Jag har valt att undersöka hondurasmahogny, europeisk valnöt, brasiliansk jakaranda, padouk och amarant, då det är träslag som ofta behöver blekas när de används i kompletteringar. Vidare undersöker jag hur den genom blekning erhållna färgen i trät i sin tur påverkas av solljus. Detta är viktigt för att förstå hur de restaurerade delarna på möbeln kommer att åldras. De viktigaste resultaten sammanfattas nedan: Valnöt, jakaranda och amarant reagerade på liknande sätt vid kemisk blekning med de i studien använda lösningarna. De blekas mest av natriumhydroxid och salter med väteperoxid. Mahogny och padouk reagerade på liknande sätt vid kemisk blekning. De blektes mest, med ett jämnt resultat, av väteperoxid med natriumhydroxid eller ammoniak. Oxidationsmedel blekte effektivare än reduktionsmedel. Enbart väteperoxid gjorde jakaranda mörkare. En hög ammoniakkoncentration gav blekare resultat än en låg på alla träslag utom amarant. Valnöt var mest ljusäkta av de undersökta träslagen. Majoriteten av mahognyproverna mörknade av solljusexponeringen. Jakaranda och padouk blektes av solljusexponeringen. Flertalet amarantprover mörknade och ett mindre antal blektes av solljusexponeringen. Solljusexperimentet identifierade blekningslösningar som gav oacceptabelt låg ljusäkthet på mahogny och padouk.   För effektiv blekning och godtagbar ljusäkthet rekommenderas följande lösningar för respektive träslag. Välj lösning beroende på önskad blekningsgrad (se Tabell 2 sid 10). Mahogny: väteperoxid, oxalsyra eller kaliumpermanganat och natriumbisulfit. Valnöt: väteperoxid med eller utan ammoniak, väteperoxid och natriumhydroxid med eller utan salter eller natriumbisulfit och oxalsyra. Jakaranda: väteperoxid med ammoniak, oxalsyra, väteperoxid och natriumhydroxid med eller utan salter, natriumbisulfit och oxalsyra eller kaliumpermanganat och natriumbisulfit. Padouk: natriumhydroxid, natriumsilikat och kalciumhydroxid följt av väteperoxid. Amarant: väteperoxid med eller utan ammoniak, väteperoxid och natriumhydroxid med eller utan salter eller kaliumpermanganat och natriumbisulfit.

From the Proceedings of the 1974 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 19-20, 1974, Flagstaff, Arizona / Two native grass species, blue grama and sidecoats, were successfully seeded on a semiarid rangeland on the walnut gulch experimental watershed in southeastern Arizona. Optimum seeding dates selected were those within the time period most likely to receive precipitation, and grass stands were established in two successive years with average rainfall. Shrubs were killed by root-plowing at a depth of 14 inches, a procedure which was more than 95% successful in controlling sprouting shrubs. Forage production measurements taken on nm-28 sideoats and Vaughn sideoats showed a yield of 1,950 and 2,643 pounds of forage per acre, respectively, for the 2 years following the seeding, whereas untreated sites produced 23 and 25 pounds per acre of forage. Results indicate that success in establishing a stand of native grass is increased through use of existing hydrologic data.

From the Proceedings of the 1973 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - May 4-5, 1973, Tucson, Arizona / Five livestock watering ponds on the walnut gulch experimental watershed were instrumented to evaluate the use of these ponds as a method for comparing rainfall amounts with runoff sediment volumes. Pond drainage area, vegetative cover, soil type, percent slope, and years of record were tested. Instrumentation consisted of water level recorders, and a topographic survey of each stock pond to ascertain its storage capacity. The results to date have been insufficient to reach definite conclusions due to instrumentation and surveying problems, and because of the natural variability of thunderstorm rainfall. Since most of these problems have now been corrected, future data should yield valuable hydrologic data for semiarid rangelands by means of these instrumented stock ponds.

From the Proceedings of the 1974 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 19-20, 1974, Flagstaff, Arizona / An erosion prediction method that has recently received wide attention in the United States is the universal soil loss equation which is given as: a=rklscp. Where a = estimated soil loss (tons/acre/year), r = a rainfall factor, k = a soil erodibility factor, l = a slope length factor, s = a slope gradient factor, c = a cropping-management factor, and p = an erosion control practice factor. Data collected on the walnut gulch experimental watershed in southeastern Arizona were used to estimate these factors for semiarid rangeland conditions. The equation was then tested with data from watersheds of 108 and 372 acres. The predicted value of annual sediment yield was 1.29 tons/acre/year as compared with an average 1.64 tons/acre/year for 4 years of data for the 108-acre watershed, and a sediment yield of 0.39 tons/acre/year was predicted for the 372-acre watershed as compared with the measured value of 0.52 tons/acre/year. Although good agreement was noted between predicted and actual sediment yield, additional work is needed before the equation can be applied to other areas of the southwest.

<div>Anthropogenically driven global change is disrupting ecosystems and habitats of many plant species, straining the ability of native species to survive and reproduce. The overarching goal of this research was to holistically work towards restoration of a threatened tree species by connecting research from different disciplines. In order to do so, the threatened butternut tree (<i>Juglans cinerea</i>) and its hybrids were used as a case study. Hybridization can incorporate stress tolerance in plants and could be a potential restoration tool. Evidence in some wild butternut populations indicates that naturalized hybrids of butternut with Japanese walnut (<i>Juglans ailantifolia</i>) may be more tolerant to butternut canker disease (BCD) than butternut, but this has not been formally tested. Thus, chapter 2 examined potential BCD tolerance within and between unadmixed and hybrid butternut inoculated with two BCD fungal isolates. Differences in canker growth were observed by fungal isolate, which could help to explain some differences in BCD severity found among butternut populations. Smaller and fewer cankers and greater genetic gains were detected in hybrid families, demonstrating that hybrids warrant further evaluation as a possible breeding tool for developing BCD-resistant butternut trees.</div><div>However, even with increased disease tolerance, hybrids must possess similar ecophysiological tolerances to their native progenitor to be an effective replacement. Butternut is extremely cold hardy, but Japanese walnuts are native to a warmer ecosystem, indicating potential disparities in extreme temperature tolerances between the two species and their hybrids. Thus, samples from mature trees were subjected to cold and heat treatments to compare relative extreme temperature tolerances within butternut and between butternut, Japanese walnut, and their hybrids. Within butternut, trees from colder areas exhibited less cold damage than those from warmer areas. Differences in heat damage among provenances occurred but did not follow a clear trend. Butternut exhibited greatest cold tolerance, Japanese walnut exhibited greatest heat tolerance, and hybrids were intermediate. Thus, the utility of hybrids for restoration could be limited at the extremes of the species’ distributions.</div><div>A second, but different type of freeze test was conducted for chapter 4 using seedlings to gain a more nuanced understanding of cold tolerance within butternut and between butternut and its hybrids. No survival or damage differences were detected in butternut provenances, although seedlings from the coldest provenances experienced more delayed budbreak at the two warmest treatments than those from warmer provenances. Interspecific differences were not observed in dieback but were detected in survival and budbreak. The hybrids had greater survival than butternut from warmer provenances at the lowest temperature treatment (-38 °C), but given that temperatures that low are extremely unlikely to occur in those provenances, it is not anticipated to give the hybrids an advantage if planted in those areas. However, the hybrids’ earlier budbreak could limit the success of restoration with these hybrids in the coldest extents of butternut’s range. </div><div>If hybrids, as well as genetically modified (GM) trees, are successfully developed for effective disease tolerance and to serve as an ecologically suitable replacement, success of restoration using hybrids will ultimately depend on those directly responsible for replanting efforts. A survey was administered to land managers in 46 organizations in Indiana to gauge perceptions of hybrid and GM trees, as well as current use of hybrid trees. Land managers had stronger concern for ecological, rather than economic, issues. Agreement was highest for using hybrid and GM trees for “conservation and restoration of at-risk species”, “timber production”, and “non-timber products (fruit, syrup, etc.)”. However, perceptions varied by characteristics, such as concern type, age, and the type of land they managed. Ecological concern and the type of land being managed most strongly predicted current hybrid use. Overall, results indicate the majority of land managers in Indiana would likely be agreeable to recommendations towards using hybrids. However, most nonetheless had strong ecological concerns about their suitability as a native replacement. It is important to note, though, that consistent with the results of previous studies, great variation was seen within the performance and characteristics of the butternut hybrids in chapters 2-4. Thus, it may be possible with careful selection and breeding to harness this variation to develop disease tolerant and ecologically similar hybrids acceptable to land managers.</div>

<p>Eastern black walnut (<i>Juglans nigra</i> L.) ranks among the most highly valued timber species in the central hardwood forest and across the world. This valuable tree fills a critical role in native ecosystems as a mast bearing pioneer on mesic sites. Along with other <i>Juglans</i> spp. (Juglandaceae), <i>J. nigra</i> is threatened by thousand cankers disease (TCD), an insect-vectored disease first described in 2009. TCD is caused by the bark beetle <i>Pityophthorus juglandis</i> Blackman (Corthylini) and the phytopathogenic fungus <i>Geosmithia morbida</i> Kol. Free. Ut. & Tiss. (Bionectriaceae). Together, the <i>P. juglandis</i>-<i>G. morbida</i> complex has expanded from its historical range in southwest North America throughout the western United States (U.S.) and Europe. This range expansion has led to widespread mortality among naïve hosts <i>J. nigra</i> and <i>J. regia</i> planted outside their native distributions.</p> <p> The severity of TCD was previously observed to be highest in urban and plantation environments and outside of the host native range. Therefore, the objective of this work was to provide information on biotic and abiotic environmental factors that influence the severity and impact of TCD across the native and non-native range of <i>J. nigra</i> and across different climatic and management regimes. This knowledge would enable a better assessment of the risk posed by TCD and a basis for developing management activities that impart resilience to natural systems. Through a series of greenhouse-, laboratory- and field-based experiments, environmental factors that affect the pathogenicity and/or survival of <i>G. morbida</i> in <i>J. nigra</i> were identified, with a focus on the microbiome, climate, and opportunistic pathogens. A number of potentially important interactions among host, vector, pathogen and the rest of the holobiont of TCD were characterized. The <i>holobiont</i> is defined as the whole multitrophic community of organisms—including <i>J. nigra</i>, microinvertebrates, fungi and bacteria—that interact with one another and with the host.</p> <p>Our findings indicate that interactions among host, vector, pathogen, secondary pathogens, novel microbial communities, and novel abiotic environments modulate the severity of TCD in native, non-native, and managed and unmanaged contexts. Prevailing climatic conditions favor reproduction and spread of <i>G. morbida</i> in the western United States due to the effect of wood moisture content on fungal competition. The microbiome of soils, roots, and stems of trees and seedlings grown outside the host native range harbor distinct, lower-diversity communities of bacteria and fungi compared to the native range, including different communities of beneficial or pathogenic functional groups of fungi. The pathogen <i>G. morbida</i> was also associated with a distinct community of microbes in stems compared to <i>G. morbida</i>-negative trees. The soil microbiome from intensively-managed plantations facilitated positive feedback between <i>G. morbida</i> and a disease-promomting endophytic <i>Fusarium solani</i> species complex sp. in roots of <i>J. nigra</i> seedlings. Finally, the nematode species <i>Bursaphelenchus juglandis</i> associated with <i>P. juglandis</i> synergizes with <i>G. morbida</i> to cause foliar symptoms in seedlings in a shadehouse; conversely, experiments and observations indicated that the nematode species <i>Panagrolaimus</i> sp. and cf. <i>Ektaphelenchus</i> sp. could suppress WTB populations and/or TCD outbreaks.</p> <p>In conclusion, the composition, function, and interactions within the <i>P. juglandis</i> and <i>J. nigra</i> holobiont play important roles in the TCD pathosystem. Managers and conservationists should be aware that novel associations outside the host native range, or in monocultures, intensive nursery production, and urban and low-humidity environments may favor progression of the disease through the effects of associated phytobiomes, nematodes, and climatic conditions on disease etiology. Trees in higher diversity, less intensively managed growing environments within their native range may be more resilient to disease. Moreover, expatriated, susceptible host species (<i>i.e.</i>, <i>J. nigra</i>) growing in environments that are favorable to novel pests or pest complexes (<i>i.e.</i>, the western U.S.) may provide connectivity between emergent forest health threats (<i>i.e.</i>, TCD) and native host populations (<i>i.e.</i>, <i>J. nigra</i> in its native range).</p>