Includes bibliographical references (l5 leaves) / vi, 126, [49] leaves, [17] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The aim of the work presented in this thesis was to develop molecular genetic markers for YD2 (the gene in barley which provides protection against barley yellow dwarf luteovirus) which could be used for the marker assisted selection of the gene in breeding programs and enable the gene to be cloned via a map-based approach. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1998

Improving our understanding of the forces driving population decline and the processes that affect the dynamics of threatened populations is central to the success of conservation management. The application of genetic tools, including our ability to examine ancient DNA, has now revolutionised our ability to investigate these processes. The recent human settlement of the Pacific, particularly in New Zealand, provides a unique, accessible system for revealing anthropogenic impacts on native biota. In this thesis I use genetic analyses from modern, historic and subfossil DNA to investigate temporal and spatial genetic structuring of the endangered yellow-eyed penguin (Megadyptes antipodes), and use these analyses to answer questions related to the conservation of this species. The yellow-eyed penguin is endemic to the New Zealand region and currently breeds on the subantarctic Auckland and Campbell Islands and the southeast coast of the South Island. The current total population size is estimated around 6000-7000 individuals, of which more than 60% inhabit the subantarctic. Despite intensive conservation measures by governmental and local community agencies, population sizes have remained highly unstable with strong fluctuations in numbers on the South Island. The species was believed to be more widespread and abundant before human colonisation of New Zealand, thus current management assumed the mainland population to be a declining remnant of a larger prehistoric population. Genetic and morphological analyses of subfossil, historic and modern penguin samples revealed an unexpected pattern of penguin extinction and expansion. Only in the last few hundred years did M. antipodes expand its range from the subantarctic to the New Zealand mainland. This range expansion was apparently facilitated by the extinction of M. antipodes' previously unrecognised sister species, M. waitaha, following Polynesian settlement in New Zealand. The demise of M. waitaha is the only known human-mediated extinction of a penguin species. Despite M. antipodes' recent range expansion, genetic analyses of microsatellite markers reveal two genetically and geographically distinct assemblages: South Island versus subantarctic populations. We detected only two first generation migrants that had dispersed from the subantarctic to the South Island, suggesting a migration rate of less than 2%. Moreover, the South Island population has low genetic variability compared to the subantarctic population. Temporal genetic analyses of historic and modern penguin specimens further revealed that the harmonic mean effective population size of the M. antipodes South Island population is low (<200). These findings suggest that the South Island population was founded by only a small number of individuals, and that subsequent levels of gene flow have remained low. Finally, we present a novel approach to detect errors in historic museum specimen data in cases where a priori suspicion is absent. Museum specimens provide an invaluable resource for biological research, but the scientific value of specimens is compromised by the presence of errors in collection data. Using individual-based genetic analysis of contemporary and historic microsatellite data we detected eight yellow-eyed penguin specimens with what appear to be fraudulently labelled collection locations. This finding suggests errors in locality data may be more common than previously suspected, and serves as a warning to all who use archive specimens to invest time in the verification of specimen data. Overall, yellow-eyed penguins have a remarkable dynamic history of recent expansion, which has resulted in two demographically independent populations. These results reveal that anthropogenic impacts may be far more complex than previously appreciated.

Some mounted ill. Bibliography: leaves 156-186.

In order to elucidate the component(s) of yellow peas responsible for their health benefits, the effects of 10 or 20 g of isolated yellow pea protein (P10 and P20) or fibre (F10 and F20) on food intake (FI) at an ad libitum pizza meal served at 30 min (experiment 1) or 120 min (experiment 2), blood glucose (BG) and appetite in young healthy males (20-30 y) were investigated. In experiment 1, P20 suppressed FI compared to all other treatments and lowered cumulative FI (pizza meal kcal + treatment kcal) compared to F10. Protein treatments suppressed pre-meal (0-30 min) BG compared to control, whereas only P20 suppressed post-meal (50-120 min) BG. In experiment 2, there was no effect of treatment on any outcome measures. Thus, protein is the component responsible for the short-term effects of yellow peas on glycaemia and FI, but its second-meal effects diminish by 2 hours post-consumption.

In order to elucidate the component(s) of yellow peas responsible for their health benefits, the effects of 10 or 20 g of isolated yellow pea protein (P10 and P20) or fibre (F10 and F20) on food intake (FI) at an ad libitum pizza meal served at 30 min (experiment 1) or 120 min (experiment 2), blood glucose (BG) and appetite in young healthy males (20-30 y) were investigated. In experiment 1, P20 suppressed FI compared to all other treatments and lowered cumulative FI (pizza meal kcal + treatment kcal) compared to F10. Protein treatments suppressed pre-meal (0-30 min) BG compared to control, whereas only P20 suppressed post-meal (50-120 min) BG. In experiment 2, there was no effect of treatment on any outcome measures. Thus, protein is the component responsible for the short-term effects of yellow peas on glycaemia and FI, but its second-meal effects diminish by 2 hours post-consumption.

Several species of North American migratory songbirds reportedly experience seasonal diet shifts involving a shift from an insect diet during the breeding season to one incorporating fruits during migration and non-breeding periods but the extent to which dietary plasticity occurs in migratory songbirds is poorly quantified. Thus, I used stable carbon (ä13C) and nitrogen (ä15N) isotope analyses to evaluate the timing and extent of frugivory throughout the annual life cycle of 16 species of migratory songbirds, representing wide ranges in body size and reported diets. Birds were sampled during spring and fall migration at the Delta Marsh Bird Observatory in 2003. To investigate dietary patterns, I sampled multiple tissues (muscle, liver, whole blood, claws, bone collagen, feathers) as these represent different periods of diet integration due to varying elemental turnover rates.<p>Assuming that relatively low ä15N values represent a fruit diet and relatively high ä15N values represent an insect diet, I expected tissues representing fall migration (liver, blood, and muscle from fall-captured birds) and winter (greater coverts and claws from spring-captured birds) to have lower ä15N values than tissues representing spring migration (liver, blood, and muscle from spring-captured birds) and summer (tail feathers and claws from fall-captured birds) when fruits are presumed to be less common in songbird diets. Based on blood and claw ä15N values, there was no isotopic segregation of species I classified a priori as insectivores or omnivores. For most species, tissue ä15N values showed either no seasonal change or a shift opposite to my prediction (e.g., ä15N values higher in fall birds compared to spring birds). Boreal fruit ä15N values were lower than those for insects; however, ä15N values of agricultural fruits overlapped both boreal fruit and insect values suggesting that food web baselines did not conform to a simple (single) linear trophic-enrichment model. In Yellow-rumped Warblers (Dendroica coronata), within-tissue seasonal comparisons for liver, muscle and blood indicated a fruit diet during fall and winter and an insect diet during spring and summer; claws and feathers of birds captured in spring (representing winter diet) had unexpectedly high ä15N values. Diet-tissue isotopic discrimination factors associated with both a fruit diet and insect diet were taken from the literature and used to correct stable isotope values of tissues to putative diet because, currently, little is known about the nature of factors influencing discrimination factors to be used in simple linear dietary mixing models. There were differences in tissue ä13C and ä15N values depending on which discrimination factor was used. Based on mixing model results for tissue ä15N values, a higher proportion of insects vs. fruits was detected in the diet of Yellow-rumped Warblers for all tissues except muscle and claws. <p>My interpretations are contingent on the fact that the available natural history information, on which guild classifications were based, was correct and that elemental turnover rates and discrimination factors used were accurate. However, much uncertainty remains about the appropriate diet-tissue isotopic discrimination factors corresponding to fruit and insect diets. Due to extensive natural variability of stable nitrogen isotope values in food sources, possible anthropogenic influences and a lack of knowledge of the metabolic processes that can potentially affect stable isotope values, I caution against using stable isotope analysis alone to track frugivory in temperate North American migratory songbirds. Future research should focus on captive studies aimed at determining and validating discrimination factors of various tissues, particularly claws and feathers, for birds feeding on varying proportions of fruits and insects. Additionally, more information on the dietary habits of these migratory songbirds is needed, as previous estimates of insectivory and frugivory in songbirds may not be accurate.

When analyzing the policies of the John F. Kennedy administration towards the People’s Republic of China, previous historians have focused on the lack of substantive change, emphasizing the continuity of action with the prior polices of the Dwight D. Eisenhower administration. At the same time, a number of historians have noted that it was during the years Kennedy was in office that a majority of the American people began viewing communist China as a greater threat to world peace than the Soviet Union. However, none have sought to explain this sizeable shift in public opinion, or analyze its potential impact on policy. This thesis incorporates archival materials with contemporary print and visual media to make a connection between the sources of public opinion shifts and a change in the assumptions upon which U.S. China policy was based. Almost from the moment the new president assumed office, Robert Komer at the National Security Council and Chester Bowles at the State Department began pushing for changes in China policy based on the assumptions that the communist regime was not a “passing phase,” would only become more powerful and over time constitute an inexorable greater threat to U.S. interests in Asia, and that rapprochement, rather than isolation, was the best means of ameliorating this threat. Together with James Thomson, Roger Hilsman, and eventually Walt Rostow, they pushed for the adoption of what A. Doak Barnett would later term “Containment Without Isolation.” While the Sino-Soviet split accentuated charges of Chinese anti-white racism and the Great Leap Forward reinforced the sense of Mao’s irrationality, the Sino-Indian War confirmed both rising Chinese power and their leadership’s capacity for rational calculation. Meanwhile, in the popular culture, particularly motion pictures, the Yellow Peril enjoyed a revival as Chinese villains stepped to the fore, beginning to free themselves of their Soviet masters. However, while foreign Chinese were feared as never before, Chinese in America gained new acceptance. Laying the groundwork for the next five decades of China policy and enemy images, Kennedy’s Thousand Days constituted a turning point.

Yellow perch, Perca flavescens (Mitchill), were collected by trawling and gillnetting in Indiana waters of Lake Michigan near Michigan City, Indiana. Sampling was conducted during the months of June, July and August from 1984 through 1986.Growth rates and length-weight relationships were found to be considerably lower than those reported previously. Males declined 38 mm at age I, 55 mm at age II, 56 mm at age III and 35 mm at age IV comparing 1986 to 1976. Females showed similar declines for the same period at 40 mm for age I, 58 mm at age II, 66 mm at age III and 53 mm at age IV. The primary reason for the decrease in growth appears to be due to the current high population density of yellow perch in Indiana waters of Lake Michigan.Maturation of males for 1986 appears to be slower than that of 1984. All male perch were mature by age III in 1984. However, only 68x of the males were mature at the same age in 1986. In 1984, 94% of the females were mature by age IV while 86% were mature at the same age in 1986.Differences in maturation rate may be a reflection of the reduced growth rates. Size at maturity was found to be similar for both sexes in 1984 and 1986. All females were mature by 230-239 mm in 1984 and 220-229 mm in 1986. All male perch were mature at 180-189 mm in 1984 and 220-229 mm in 1986.Total estimated average annual percent mortalities of 79, 58, 66 and 55 were calculated for combined sexes from age composition analyses end trawl catch data for 1976 and 1984-86. Yellow perch production for 100 fish was estimated for June through August using the Allen curve method. Biomass of substock ( <130 mm), stock ( >129 mm) and quality fish (200 mm or larger) were estimated using Proportional Stock Density to investigate population structure. The Allen curve biomass models were modified to more accurately reflect the trawl catch density data as an index of population changes in 1976 compared to 1984-86. Theoretical biomass for the substock component increased 23 fold from 1976 to 1986. Biomass of the stock sized fish was estimated as 15 times greater in 1986 compared to 1976. Quality fish biomass varied from year to year with the average for 1984-86 estimated as 14 times greater than 1976. The most apparent factor now influencing deterioration of growth and resultant quality of the population is decreased mortality/increased survival with increase in biomass. If the low mortality rates observed for 1984-86 remain unchanged, the data indicate continued population density increases will result in even lower growth rates, and a population dominated by a higher percentage of small, non-quality fish.Food habit analyses for 1984 were compared to a diet study of yellow perch in 1972 for the study area. Zooplankton increased from 0.4x in 1972 to 9% of the volume in 1984 for 100-175 mm perch. This trend may reflect an increase in zooplankton populations in response to the population decline of the planktivorous alewife. Young-of-the-year yellow perch made up 85% of the stomach volume for 176-225 mm perch in 1984 while none were found in samples for 1972. This clearly shows yellow perch are resorting to cannibalism resulting from excessive intraspecific competition.Diet analyses by month revealed alewife eggs were moat important during peak abundance in June and July. Yellow perch (YOY) and Pontocoreia affinis became important during August. Analysis by size interval showed zooplankton and insects (primarily Chironomidae) were important to the diet of perch in the 30-59 mm (YOY) size interval. Copepoda were important by percent volume until approximately 60 mm when the zooplankton component switched to Cladocera. Data for 60-119 mm (age I+) fish indicates alewife eggs were important during June but insects became increasingly more important during July and August. Diversity in food items consumed diminished with increase in size as diet of 120-159 mm (age II+) yellow perch consisted largely of fish. Increases in percent volume of rainbow smelt in June and July, and Y0Y perch in August for age II+ and older perch compared to younger/smaller fish may be related to increased capture success. Diet of fish larger than 160 mm (age III+ and older) consisted almost entirely of rainbow smelt and Y0Y yellow perch.Ball State UniversityMuncie, IN 47306

I examined the effects of hydrologic gradients and upland timber harvesting with different streamside management zone widths on yellow-poplar (Liriodendron tulipifera) processing and the associated macroinvertebrate community structure in the Cumberland Plateau ecophysic region, U.S.A. Prior to upland timber harvesting, 5.0 ± 0.1 g yellow-poplar leaf packs were constructed, zip-tied to gutter nails, and placed into 7 perennial and 6 temporary stream reaches with similar physiochemical and geomorphic characteristics. From December 2007 to May 2008, 3–5 leaf packs were collected per reach monthly. I found significant differences in the functional feeding group composition. Temporary reaches contained higher shredder, gathering-collector, predator, and total macroinvertebrate abundances. Shredder and total macroinvertebrate biomass was also higher in the temporary stream reaches. Gathering-collector biomass along one measurement was higher in the temporary streams. Perennial and temporary stream reaches contained similar macroinvertebrate diversity. Logging operations occurred from May 2008–December 2008. After logging operations ended, yellow-poplar leaf packs were placed into the perennial and temporary reaches of 3 control and 3 treatment streams (2 with same SMZ width, 1 different). From December 2008–May 2009. Leaf packs were collected monthly. Within the temporary and perennial stream reaches, no significant differences were detected between control and treatment yellow-poplar processing rates. No significant differences were detected between the control and treatment functional feeding group composition in abundance and biomass. Post-harvest, taxon richness increased in both the perennial control and treatment streams, while richness declined in the temporary control and increased in the temporary treatment. My findings indicate that when water is present, organic matter processing will function similarly to downstream reaches that have continual water flow. During seasonal flow patterns, macroinvertebrate communities associated with organic matter are present in temporary streams and may exceed perennial stream reaches in their density and biomass. This indicates that temporary streams are physically suitable habitats for macroinvertebrate fauna and contribute to a stream’s form and function. Overall, no observed distinct response in yellow-poplar processing rates or the associated macroinvertebrate community structure was detected within the perennial or temporary streams. Macroinvertebrate community structure varied spatially and temporally. On the taxonomic level, increases in taxa-specific abundance and biomass remain to be explained. Future research assessing interactions on the taxonomic level might help explain increases or decreases in abundance and biomass in relation to treatment effects. This study documented the response of organic matter breakdown and associated macroinvertebrate community structure during the 1st 5 months after logging. Thus, it is only a snapshot of stream ecosystem response to disturbance. Long-term studies are needed to evaluate full ecosystem response and recovery. Due to uncontrollable factors, I was not able to evaluate the success of different SMZ widths. Results documented should be treated with hesitancy, until full ecosystem response has been documented.

Introduction of fish species to North American drainages has occurred for over 100 years. Introduced fish species have been documented to have adverse effects on both the environment and native species of the drainage into which they have been introduced. To better understand the effects that introduced species may have on a particular drainage, it is essential to understand aspects of the introduced species’ life history. The objectives of the current study is to quantify the age, reproduction, growth, condition and diet of the yellow bass, Morone mississippiensis, in Barren River Lake, Kentucky. Monthly collections from three areas on Barren River Lake were made via a boat-mounted electrofisher from March 2008 to March 2009. Fish age was estimated by examining the sagittal otoliths of each individual. Reproductive condition was assessed using the mean gonadosomatic index (GSI) of all sexually mature individuals by month. Yearly growth rates were estimated by computing the mean length at age for each age class and subsequent calculation of the von Bertalanffy growth function (VBGF). To estimate the condition of yellow bass as it changed throughout the sample period, relative weight of each individual was calculated and the mean monthly relative weight was calculated. To examine the diet of yellow bass, diet items were identified to the lowest practical taxonomic level. Then, dry weight of each diet item was estimated and pooled by season to assess the season changes in the diet of yellow bass. Individuals of age group 3 were the most frequent. Mean GSI was significantly higher in March, April and May of 2008. Calculation of the VBGF yielded 254.7 mm as the maximum attainable mean total length of yellow bass in Barren River Lake. VBGF predicted mean total lengths of age classes 0-8 were as follows: 21.7 mm, 64.4 mm, 99.2 mm, 127.7 mm, 151.0 mm, 170.0 mm, 185.5 mm, 198.2 mm, and 208.5 mm. Relative weight was highest in summer. The diet of adult and sub-adult yellow bass relied heavily on chironomid larvae and pupae throughout the year, although diet item consumption was very low in winter. Young-of-year gizzard shad (Dorosoma cepedianum), however, became the most important adult diet item in the spring and summer. To better understand the impacts that the introduced yellow bass has on the ecosystem of Barren Rive Lake, a multi-year study including an estimation of relative abundance is recommended.