Acute myeloid leukaemia (“AML”) is a clonal disease characterised by increased, uncontrolled abnormal white blood cells and the accumulation of leukaemia immature cells in the bone marrow and bloodstream. Chromosomal rearrangements have been detected in almost half of AML cases. It has been proven that the chromosomal rearrangements constitute a marker for the diagnosis and prognosis of AML and have therapeutic consequences. The discovery of these rearrangements has led to a new World Health Organization (“WHO”) classification system. However, small regions of cryptic chromosomal rearrangements have been identified among these cases. Such cryptic rearrangements can be explained by the identification of small regions which cannot be found by conventional chromosome banding techniques. Moreover, approximately 50% of AML cases have been found with normal karyotypes. The improvement of cytogenetic techniques, including fluorescence in situ hybridization (“FISH”) and single nucleotide polymorphism (“SNP”) platforms, have allowed the detection of small rearranged regions (such as copy number changes) both in normal and abnormal karyotype AML. This study identifies: (i) cryptic chromosomal translocations in leukaemia cells of AML patients; (ii) DNA copy number changes in patients with known chromosomal translocations; and (iii) the proliferating state of leukemic cells harbouring chromosomal abnormalities within a series of patients. In the initial study, the FISH technique was performed on 7 AML patient samples to validate a novel three colour probe for the detection of t(7; 12). The results demonstrated that the new three-colour FISH approach used in this study has enabled the detection of a cryptic t(7;12) translocation as part of a complex rearrangement in one patient previously been described as having t(7;16) and ETV6-HLXB9 fusion transcript at the molecular level. To date there are only two cases of a cryptic t(7; 12) translocation reported in the literature. Additionally, the new three-colour FISH approach also enabled identification of t(7; 12) in a new seven year-old AML patient (the first case of childhood leukaemia with an onset after infancy to be found positive for t(7; 12)). In the second study the FISH technique was used to validate three colour probe sets for the detection of 7(q22-q31) and 7(q22-q36.1) regions on several myeloid cell lines. The results indicate that the probes found chromosome 7 rearrangements in myeloid cell lines with complex rearrangements. The three colour probe sets enabled detection of a new rearrangement in the k562 cell line, described as a duplication of 7q36 region, followed by intrachromosomal insertion of long arm material into the short arm of chromosome 7. The intrachromosomal insertion identified in k562 cell line is an uncommon form of chromosomal rearrangement in myeloid leukaemia which has not been previously reported. In the third study, the Illumina BeadArray approach was used to assess copy number alterations (“CNAs”) and copy number loss of hertrozygosity (“CN-LOH”) regions in 22 AML patients samples with inv(16)(p13;q22) and t(8;21)(q22;q22) rearrangements. In order to distinguish between true CNAs and false-positive findings as well as to verify whether CNAs are present in the same clone harbouring inv (16), FISH was used on fixed chromosome and cell suspensions from the same patients. The results showed a low number of copy number losses and copy number gains in 17 (77.27%) out of 22 cases, with an average of 1.86 CNAs per case as well as copy neutral-LOH with an average of 6.7% per patient. Furthermore, interphase FISH was carried out on four cases showing a 7q36.1 deletion, 4q35.1 deletion, 16.13.11 deletion and 8q24.21-q24.3 gain identified by array. The FISH results confirmed CNAs in most cases while CNA was not confirmed in one patient. Moreover, the FISH data analysis showed that the CNAs were found in both cells without inv (16) and cells harbouring the inv (16) rearrangement. In the final study, indirect immunofluorescence (IF) was used to determine the ki67 staining patterns in 8 stimulated and unstimulated peripheral blood samples and k562 cell lines. The results showed a high percentage of ki67 positive staining in the stimulated samples in comparison with unstimulated samples, which showed a low percentage of ki67 positive staining. In addition, a high percentage of proliferating cells were detected in the k562 cell line. ImmunoFISH was performed on five different patient samples and leukaemia cell lines using specific probes in the regions of interest to detect the chromosomal abnormalities and using the ki67 antibody to assess the proliferation state of the cells. The results showed that the proliferation state of the cells carrying chromosomal abnormalities in two patients was higher than the proliferation state of the cells carrying abnormalities in three patients; in other words, most of the cells carrying abnormalities were proliferating in two cases and non-proliferating in three cases.

International Telemetering Conference Proceedings / October 13-16, 1986 / Riviera Hotel, Las Vegas, Nevada / The Airborne Platform Telemetry Relay System (AP/TM) is currently being built for the Gulf Range Instrumentation System. The AP/TM will allow air-to-air missile test and training missions to be conducted beyond the line-of-sight of land-based instrumentation. The AP/TM is comprised of the following subsystems: C a Telemetry Data Relay C a Sea Surveillance Radar and Radar Data Link C a Drone Control Relay C a UHF Radio Relay The Telemetry Data Relay Subsystem will receive telemetry signals from five independent sources and will retransmit them to land based receiving sites. This subsystem contains a 75 square foot, electronically steerable, five beam phased array antenna and uses polarization diversity to eliminate polarization mismatch loss and to improve reception in the presence of multipath propagation. The AP/TM will also have the capability of relaying four channels of voice communications and drone tracking data and to perform sea surveillance of the mission area. The coordinates of targets detected by the radar will be relayed to the range control center over a high frequency (HF) data link. In addition to the airborne equipment, the system also includes a ground support instrumentation van which is used for pre- and post-flight checkout and maintenance.

International Telemetering Conference Proceedings / October 23-26, 2000 / Town & Country Hotel and Conference Center, San Diego, California / A thinned array sensor system develops error voltages for steering dish antennas from signals arriving over a broad range of angles, thereby eliminating need for a priori knowledge of signal location.

ITC/USA 2015 Conference Proceedings / The Fifty-First Annual International Telemetering Conference and Technical Exhibition / October 26-29, 2015 / Bally's Hotel & Convention Center, Las Vegas, NV / This paper looks at the migration paradox from a holistic perspective, revisits the Smarter Antenna concept and looks for synergetic solutions. The architecture presented also builds on the momentum of the relentless migration to network technology (that has already transformed telemetry data centers) and pushes it to the vehicle. The results are reduced costs and exciting new functionality, such as better situational awareness for mission conduct and range safety. Spatial and temporal domains are harnessed as aggressively as the frequency domain to enable denser spectral utilization and other exciting benefits. Imagine a Test Range no longer reliant on tracking systems (almost)!

ITC/USA 2015 Conference Proceedings / The Fifty-First Annual International Telemetering Conference and Technical Exhibition / October 26-29, 2015 / Bally's Hotel & Convention Center, Las Vegas, NV / In this work, a 3D-printable multilayer phased array system is designed to demonstrate the applicability of additive manufacturing technique combining dielectric and conductor processes at room temperature for RF systems. Phased array systems normally include feeding networks, antennas, and active components such as switches, phase shifters and amplifiers. To make the integrated system compact, the array system here uses multilayer structure that can fully utilize the 3D space. The vertical interconnections between layers are carefully designed to reduce the loss between layers. Simulated results show good impedance matching and high-directive scanning beam. This multilayer phased array will finally be 3D printed by integrating thermal / ultrasound wire mesh embedding method (for metal) and fused-deposition-modeling technique (for dielectric).

The pole-dipole (PD) electrical resistivity array is used as part of a geophysical technique, which can be used in exploration for mineral, geothermal and hydrological resources. Furthermore, it can be used in archaeological investigations. The PD array is useful in obtaining large depths of investigation, but the array requires one pole to be planted at a greater distance from the other electrodes. Placing the infinite pole is time consuming and costly, especially in rough terrains. It can also be a liability in populated areas. Previous data inversion software assumes the infinite pole to be planted at infinity. Therefore, the field data collected has to mimic the assumptions of the inversion software. Some recent inversion programs use all 4-electrode positions for data inversion. In this case, is a good approximation of infinity for the infinite pole still necessary? Is the depth of investigation still the same or relevant with a non-infinite, infinite pole? Is the resolution of the cross-sections retained? To answer the above questions a Generalized Electrode Array (GEA) 1 dimensional (1D) inversion program is used. Different sets of field data were collected with non-infinite pole (NIP) PD arrays; these arrays are set up as PD arrays with an infinite pole planted relatively close to or on the survey lines itself. A COMSOL finite element model were modelled with varying infinite pole distances to identify the distance required for the retention of the depth of investigation and resolution of the PD array. Modelling from GEA shows the NIP distances do not affect the 1D inversion accuracy of the resistivity nor the layer thickness. The field data indicates that it is necessary to have an infinite pole, but the distance to the infinite pole can be substantially less than 10 times the array length, which is the usual rule of thumb for infinite pole placement. With 3 dimensional (3D) COMSOL modelling results, it indicates a minimum pole distance to be 2.5-array length to retain the depth of investigation and precision of the inverted sections of the PD array.

International Telemetering Conference Proceedings / October 22-25, 2001 / Riviera Hotel and Convention Center, Las Vegas, Nevada / Multiple same-frequency signals including direct/multipath signals are distinguished and individually tracked by measuring phase differences between sum and error channels of thinned array systems.

Thesis (PhD)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: The development of a tool kit for the design of superconducting programmable gate arrays (SPGAs) is discussed. A circuit optimizer using genetic algorithms is developed and evaluated. Techniques and a program are also developed for the generation of segmentized 3D models with which to calculate inductance in circuit structures through FastHenry. The ability to add random variations to the dimensions of the models is included. These tools are then used to design novel latching elements that allow the construction of reprogrammable Rapid Single Flux Quantum (RSFQ) circuits. A circular process is used, whereby layouts are converted back to circuit diagrams through element extraction, and reoptimized if necessary. Two programmable frequency dividers are then designed; one for testing the routing and switch structures and programming architecture of an SPGA, and another compact one for testing the latching elements and off-chip interface. The dissertation concludes with an overview of the circuits necessary for the implementation of a fully functional SPGA. / AFRIKAANSE OPSOMMING: Die ontwikkeling van ’n gereedskapstel vir die ontwerp van supergeleier FPGA’s (SPGA’s) word bespreek. Eerstens word ’n stroombaanoptimeerder, wat met genetiese algoritmes funksioneer, ontwikkel en geëvalueer. Daarna word tegnieke en ’n program ontwikkel om driedimensionele segmentmodelle te genereer waaruit FastHenry die induktansie van stroombaanstrukture kan bepaal. Die vermoë om toevalsveranderinge by die dimensies van die modelle te voeg, is ook ingesluit. Hierdie gereedskap word dan gebruik om nuwe grendelelemente te ontwerp waarmee herprogrammeerbare Rapid Single Flux Quantum (RSFQ) stroombane gebou kan word. ’n Sirkulêre proses word gevolg, waarvolgens uitlegte na stroombaandiagramme teruggeskakel kan word (deur elementonttrekkings) en, indien nodig, heroptimeer kan word. Twee programmeerbare frekwensiedelers word daarna ontwerp; een om die pulsvervoer- en skakelstrukture, asook programmeringsargitektuur van ’n SPGA te toets, en ’n ander, kompakter een om die grendelelemente en warmlogika koppelvlakke mee te toets. Die proefskrif sluit af met ’n oorsig oor die stroombane benodig vir die implementering van ’n volledig funksionele SPGA.

Thesis (MScEng (Electrical and Electronic Engineering))--University of Stellenbosch, 2006. / The design of an electrically small planar antenna for compact circular polarization is presented. After an in-depth study of the performance limitations on electrically small antennas and an investigation into the working of various existing electrically small antennas, the design, simulation and measurement of the proposed antenna element is presented in detail.

The prefrontal cortex (PFC) is an area of the brain that is critically important for learning, memory, organization, and integration, and PFC dysfunction has been associated with pathologies including Alzheimer’s disease, schizophrenia, and drug addiction. However, there exists a paucity of information regarding neurochemical signaling in the distinct sub-regions of the PFC, particularly the medial prefrontal cortex (mPFC). The mPFC receives glutamatergic input from a number of brain areas, and functional glutamate signaling is essential for normal cognitive processes. To further understand glutamate neurotransmission, in vivo measurements of glutamate were performed in the cingulate cortex, prelimbic cortex, and infralimbic cortex of anesthetized rats using enzyme-based microelectrode array technology. Measurements of acetylcholine were also performed to examine the relationship between glutamate and other neurotransmitters in the mPFC. The described studies revealed a homogeneity of glutamate and acetylcholine signaling in the mPFC sub-regions, indicating somewhat uniform tonic and phasic levels of these two transmitters. In the infralimbic mPFC of awake freely-moving rats, rapid, phasic glutamate signaling events, termed “transients” were observed and in vivo glutamate signaling was successfully monitored over 24 hour time periods. The effects of methylphenidate (MPH), a stimulant medication with abuse potential that is used in the treatment of attention-deficit hyperactivity disorder, were measured in mPFC sub-regions of anesthetized rats. Data revealed similar tonic and phasic glutamate levels between chronic MPH-treated rats and controls in all sub-regions. Locomotor data from the chronic treatment period supported the behavioral sensitization effects of multiple MPH treatments. Significant effects were observed in locomotor activity, resting levels of glutamate, and glutamate uptake rates in the infralimbic mPFC of awake, freely-moving animals that received chronic MPH treatment. Taken together, this body of work characterizes glutamate signaling in the rat mPFC to a degree never before reported, and serves to report for the first time the effects of MPH on glutamate signaling in the mPFC.