La nanotechnologie possède la potentialité d’améliorer la sécurité, les procédés, l’emballage alimentaire et le concept d’ingrédient fonctionnel. La nano-encapsulation d’agents actifs, est un concept innovant permettant de protéger les agents actifs d’une dégradation éventuelle pendant le procédé de fabrication de l’aliment et son stockage. Le principal objectif de ce travail est de développer et d’optimiser une méthode de marquage fluorescent afin d’effectuer des études de transfert dans différents films de bio-polymères et dans l’aliment et de nano-encapsuler la nisine. La nano-encapsulation de la nisine dans différents nano-liposomes par micro-fluidisation (CCDS) est une technique innovante pour la fabrication de nano-systèmes de re-largage. L’incorporation de nisine sous forme de nano-émulsion est une méthode efficace de contrôle des flores pathogènes sans altérer les caractéristiques des films d’HPMC. La nisine Z a été marquée par un composé fluorescent, et a une masse moléculaire de 3713,3. Des études en microscopie confocale ont permis de démontrer que l’interaction de la nisine avec les membranes bactériennes se situait au niveau de site de division de la cellule. L’HPMC, le chitosane, le caséinate et l’acide poly-lactique agissent comme des réservoirs et libèrent progressivement la nisine afin d’obtenir un effet inhibiteur durable. Le choix du biopolymère affecte la biodispinibilité du composé à la surface et à l’intérieur de l’aliment. La prochaine révolution concernant la sécurité alimentaire par l’emballage mettra en avant le dernier concept technologique « 3-BIOs » qui se réfère aux notions Bioactif - Biodégradable - Bionanocomposite / Food nanotechnology has the potential to improve food safety and bio-security, food processing, food packaging and functional ingredients. Nano-encapsulation of active agents is an innovative concept to protect them against possible denaturation during processing and storage. The overall objective of the present work was to optimize and develop fluorescent labeling and encapsulation of nisin for molecular transfer study in different packaging based on biopolymers and in the food. Nanoencapsulation of nisin in different nanoliposomes by using continuous cell disruption system (CCDS) has provided an innovative method for nano-delivery systems fabrication. Incorporation of nisin in nano-emulsion form (encapsulated and free) can possibly be an effective approach to control pathogen without compromising the basic physico-chemical attributes of composite HPMC coatings. The fluorescently labeled nisin Z prepared had a molecular weight of 3717.3 Da. Confocal microscopic studies demonstrated the interaction of nisin with the bacterial membranes at the cell-division sites as possible mechanism of action against food borne pathogen. HPMC, CTS, SC and PLA packaging bio-membranes act as a reservoir and progressively release nisin to sustain a constant inhibitory effect. Choice of biopolymer is significant in providing requisite bioavailability of antimicrobial compounds at exterior surface and inside the food system. Based on the present study results, the emerging revolution concerning food safety through packaging possibly will rely on « 3-BIOs » blend with nanotechnology, which refers to Bioactive, Biodegradable and Bio-nanocomposite

The aim of this research was to study the effect of pre-treatment and modification processes on the properties of hemp cellulose fibre for biopolymer composites application. Hemp fibres have been modified by various extraction, swelling, chemical and enzymatic treatments. The morphology and mechanical properties of the modified fibres have been measured. Biopolymer composites have been prepared using the modified fibres and matrices of cellulose acetate butyrate and cellulose solutions derived from hemp. The first fibre treatment employed was acetone extraction and mercerization. A low pressure acrylonitrile grafting initiated by azo-bis-isobutylonitrile was performed using alkali treated fibre. The AN grafted fibres had no transformation of crystalline structure as observed after mercerization. The mechanical properties performed by a single fibre test method were strongly influenced by the cellulose structure, lateral index of crystallinity, and fraction of grafting. Bioscouring of hemp using pectate lyase (EC 4.2.2.2), Scourzyme L, was performed. Greater enzyme concentration and a longer treatment improved the removal of the low methoxy pectin component. Removal of pectate caused no crystalline transformation in the fibres, except for a slight decline in the X-ray crystalline order index. Smooth surfaces and separated fibres were evidence of successful treatment. The shortening of fibre by grinding and ball-milling was introduced to achieve a desired fibre size. An increase in the milling duration gradual ly destroyed the crystalline structure of the cellulose fibres. An increase in solvent polarity, solvent-fibre ratio, agitation speed and drying rate resulted in the rearrangement of the ball-milled cellulose crystalline structure to a greater order. The thermal degradation behaviour of hemp fibres was investigated by using TGA. The greater activation energy of treated hemp fibre compared with untreated fibre represented an increase in purity and improvement of structural order. The all hemp cellulose composites were prepared by an introduction of fibres into 12% cellulose N-methyl-morpholine N-oxide (NMMO) solution and water-ethanol regeneration. A broadening of the scattering of the main crystalline plane, (002) and a depression of the maximum degradation temperature of the fibres were observed. These revealed a structural change in the fibres arising from the preparation. The mechanical properties of composites depended on size, surface area, crystallinity and the structural swelling of the fibres. Composites of cellulose acetate butyrate (CAB) and modified hemp fibres were prepared. Composites containing pectate lyase enzyme treated fibres showed better mechanical property improvement than untreated and alkali treated fibres respectively.

Clay minerals have historically played a consequential role in human health. While the beginnings were rooted in geophagy, a primitive act of consuming earth, the health-related uses of clay minerals have evolved and diversified over time. As excipients in pharmaceutical formulations, clay minerals can attribute novel properties onto intercalated compounds. Intercalating oxybenzone, a UV filter, within the interlamellar space of montmorillonite is desirable in order to minimize direct contact with skin. Intercalating resveratrol, a compound known for attributing beneficial effects onto human health, may be advantageous since this compound is susceptible to cis-trans isomerisation. The strategy of using alkylammonium–modified clay was undertaken and proved successful for the intercalation of oxybenzone. The field of biopolymer/layered silicate nanocomposites is heavily researched for use in a multitude of applications. Novel montmorillonite nanocomposites were prepared with neutral guar gum and cationic guar gum, using an environmentally friendly process and are fully characterized.

Clay minerals have historically played a consequential role in human health. While the beginnings were rooted in geophagy, a primitive act of consuming earth, the health-related uses of clay minerals have evolved and diversified over time. As excipients in pharmaceutical formulations, clay minerals can attribute novel properties onto intercalated compounds. Intercalating oxybenzone, a UV filter, within the interlamellar space of montmorillonite is desirable in order to minimize direct contact with skin. Intercalating resveratrol, a compound known for attributing beneficial effects onto human health, may be advantageous since this compound is susceptible to cis-trans isomerisation. The strategy of using alkylammonium–modified clay was undertaken and proved successful for the intercalation of oxybenzone. The field of biopolymer/layered silicate nanocomposites is heavily researched for use in a multitude of applications. Novel montmorillonite nanocomposites were prepared with neutral guar gum and cationic guar gum, using an environmentally friendly process and are fully characterized.

Clay minerals have historically played a consequential role in human health. While the beginnings were rooted in geophagy, a primitive act of consuming earth, the health-related uses of clay minerals have evolved and diversified over time. As excipients in pharmaceutical formulations, clay minerals can attribute novel properties onto intercalated compounds. Intercalating oxybenzone, a UV filter, within the interlamellar space of montmorillonite is desirable in order to minimize direct contact with skin. Intercalating resveratrol, a compound known for attributing beneficial effects onto human health, may be advantageous since this compound is susceptible to cis-trans isomerisation. The strategy of using alkylammonium–modified clay was undertaken and proved successful for the intercalation of oxybenzone. The field of biopolymer/layered silicate nanocomposites is heavily researched for use in a multitude of applications. Novel montmorillonite nanocomposites were prepared with neutral guar gum and cationic guar gum, using an environmentally friendly process and are fully characterized.

Thesis (MSc)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Biopolymers exhibit the required material properties to replace conventional, non-biodegradable, petroleum-based polymer products. They have a closed carbon cycle, making them carbon neutral and environmentally friendly. Biopolymers are produced from non-toxic substrates during in vivo enzymatic reactions. Biosynthesis of the most commercially important biopolymers is too complex to be reproduced in in vitro reactions. Identification of the genes responsible for their biosynthesis has been under investigation, with some pathways already elucidated. The genes involved in the biosynthesis of these polymers have been targeted for genetic manipulation to increase productivity, as well as create tailor-made polymers. Novel biopolymers and the genes responsible for their synthesis are of interest for their potential commercial applications. Bacteria produce a wide range of biopolymers and are being implemented as the bio-factories for biopolymer production. They are capable of utilising easily accessible and renewable carbon sources such as sucrose for polymer biosynthesis. Bacteria thus allow for economical production of these environmentally beneficial polymers. In this study, the gene responsible for the production of an unknown biopolymer from an unknown bacterium was identified. The biopolymer producing bacteria were grown on media enriched with sucrose as carbon source, during an expression library screening in a previous study. Expression library technology was used to search for the gene and it was identified as a 424 amino acid levansucrase which had a 100% homology to Leuconostoc mesenteroides M1FT levansucrase (AAT81165.1). Biopolymer analysis revealed that the biopolymer was a levan, a polysaccharide consisting of only fructose molecules with a molecular weight of ± 5 kDa. Analysis of a 516 bp fragment of the 16S rRNA determined that the unknown bacteria were a Pseudomonas species. / AFRIKAANSE OPSOMMING: Bio-polimere besit noodsaaklike materiële eienskappe wat toelaat dat dit konvensionele, nie bio-afbreekbare, petroleum-gebasseerde polimeer produkte kan vervang. Hulle het n geslote koolstof kringloop en is dus koolstof neutraal en omgewingsvriendelik. Bio-polimere word vervaardig van nie-toksiese substrate, gedurende ensiematiese reaksies in vivo. Die belangrikste kommersiële bio-polimere se ensiematiese produksie is te kompleks om in ʼn in vitro reaksie te herproduseer. Ondersoeke tot die identifikasie van die gene wat verantwoordelik is vir die produksie van die polimere is onderweg, en sommige produksie paaie is reeds bekend. Die bekende gene word geteiken vir genetiese manipulasie om hulle produktiwiteit te vermeerder en om unieke polimere te produseer. Unieke bio-polimere en die gene wat vir hul produksie verantwoordelik is, is van belang vir hulle potentiële implimentering in komersiële toepassings. Bakteria produseer ʼn verskeidenheid bio-polimere en word as die bio-fabrieke vir polimeerproduksie geimplimenteer. Hulle kan maklik bekombare koolstofbronne, soos sukrose, gebruik om bio-polimere te produseer. Bakteria laat dus die ekonomiese produksie van hierdie omgewingsvriendelike polimere toe. In hierdie studie word die geen wat verantwoordelik is vir die produksie van ʼn onbekende bio-polimeer van ʼn onbekende bakteria, geidentifiseer. Die bakteria was gevind op media, wat verryk was met sukrose as koolstofbron, tydens ʼn vorige studie, waartydens ʼn uitdrukkingsbiblioteek gesif was op hierdie media. Uitdrukkingsbiblioteek tegnologie was gebruik om die geen te vind. Die geen was geidentifiseer as ʼn 424 aminosuur, homo-fruktose-polimeer produseerende geen, ʼn “levansucrase”. Die geen het ʼn 100% homologie met die M1FT “levansucrase” geen (AAT81165.1) van Leuconostoc mesenteroides gehad. Analise van die bio-polimeer het bepaal dat die polimeer ʼn polisakkaried was, wat slegs uit fruktose molekules bestaan het. Die molekulêre gewig van die polimeer was ± 5 kDa. Analise van ʼn 516 bp fragment van die 16S rRNS het bepaal dat die bakteria van die Pseudomonas spesie afkomstig was.

Submitted by MARCIO TULIO MATHEUS SANTOS null (marciotulio@globo.com) on 2018-04-03T12:14:14Z No. of bitstreams: 1 DISSERTAÇÃO - MARCIO TULIO - 26-03-2018 CORRIGIDA VF.pdf: 2812609 bytes, checksum: 292ece5efee66c9b2d09ec3d1956148c (MD5) / Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2018-04-03T12:55:34Z (GMT) No. of bitstreams: 1 santos_mtm_me_bot.pdf: 2812609 bytes, checksum: 292ece5efee66c9b2d09ec3d1956148c (MD5) / Made available in DSpace on 2018-04-03T12:55:34Z (GMT). No. of bitstreams: 1 santos_mtm_me_bot.pdf: 2812609 bytes, checksum: 292ece5efee66c9b2d09ec3d1956148c (MD5) Previous issue date: 2018-02-23 / Os biopolímeros podem combinar a degradabilidade “in vivo” com a boa biocompatibilidade e, desta forma, podem ser usados como agentes encapsulantes com o objetivo de libertarem compostos bioativos. Dentro do grupo dos biopolímeros, os polissacarídeos apresentam vantagens importantes, como alta estabilidade, segurança, hidrofilicidade e biodegradabilidade. Os polissacarídeos têm sido objetos de estudos, apresentando resultados promissores. Este trabalho tem como objetivo, analisar o comportamento de uma matriz biopolimérica a base de amido, quando submetida à radiação ionizante visando seu uso em moldes planares para radioterapia de lesões cutâneas. Foram confeccionadas amostras de matrizes biopoliméricas de amido, constituído de água, amido, glicerina, duas variedades de açúcares na forma de pó, totalmente solúveis em água e produzida através da conversão do amido de milho. Durante o preparo das amostras variou-se as formulações para se obter melhor resistência da película plástica formada. As formações dos filmes de amido, foram realizadas através do método Casting com baixa viscosidade. As amostras foram submetidas à radiação em um acelerador linear clínico (linac) com feixes de raios-X de 10 MV e feixe de elétrons de 4 MeV, 6 MeV, 9 MeV e 12 MeV, e em uma fonte telecobaltoterapia com 1,25 MeV de energia. A dose total de radiação aplicada variou de 20 Gy a 140 Gy, um grupo controle foi mantido sem exposição à radiação. Após o processo de irradiação, foi realizada uma dosimetria das amostras, usando câmaras de ionização e detector Geiger Muller, não houve leitura de radiação residual nas amostras. Em seguida, as amostras foram submetidas a análise espectrofotométrica para avaliação de sua estabilidade e verificar se a radiação induzida alterou os grupos orgânicos e a estrutura primária do biomaterial. A análise de FTIR, mostrou que a radiação aplicada sobre o molde biopolimérico não alterou sua estrutura ou grupos químicos, sendo preservadas as propriedades plastificantes do biomaterial. / Biopolymers can combine "in vivo" degradability with good biocompatibility and, in this way, can be used as encapsulating agents in order to release bioactive compounds. Within the group of biopolymers, polysaccharides have important advantages, such as high stability, safety, hydrophilicity and biodegradability. Polysaccharides has been object of studied, presenting promising results. This work aims to study the properties of biopolymers for use in pharmaceutical applications in the treatment of pathologies and to analyze the behavior of the biopolymer matrix based on starch when submitted to ionizing radiation for its use in planar models for radiotherapy of cutaneous lesions. An extensive bibliographic research was carried out aiming to understand the characteristics, properties and applications of the biopolymers in the health area, having as starch the base of the polymer matrices. Samples of biopolymeric were made matrices, consisting of water, starch, glycerin, two sugar varieties in the form of powder, totally soluble in water and produced through the conversion of corn starch were made. During the preparation of the samples the formulations were varied to obtain better resistance of the plastic film formed. The formations of the starch films were carried out using the casting method with low viscosity. The samples were subjected to radiation in a clinical linear accelerator (linac) with x-ray beams of 10 MV and electron beam of 4 MeV, 6 MeV, 9 MeV and 12 MeV, and in a telecobaltotherapy source with 1.25 MeV of energy. The total applied radiation dose varied from 20 Gy at 140 Gy, a control group was maintained without radiation exposure. After the irradiation process, a dosimetry of the samples was carried out, using ionization chambers and Geiger Muller detector, there was no residual radiation reading in the samples. The samples were then subjected to spectrophotometric analysis to evaluate their stability and to verify if the induced radiation altered the organic groups and the primary structure of the biomaterial. The FTIR analysis showed that the radiation applied on the biopolymer mold did not alter its structure or chemical groups, and the plasticizing properties of the biomaterial were preserved.

Orientador: Marco Antônio Rodrigues Fernandes / Resumo: Os biopolímeros podem combinar a degradabilidade “in vivo” com a boa biocompatibilidade e, desta forma, podem ser usados como agentes encapsulantes com o objetivo de libertarem compostos bioativos. Dentro do grupo dos biopolímeros, os polissacarídeos apresentam vantagens importantes, como alta estabilidade, segurança, hidrofilicidade e biodegradabilidade. Os polissacarídeos têm sido objetos de estudos, apresentando resultados promissores. Este trabalho tem como objetivo, analisar o comportamento de uma matriz biopolimérica a base de amido, quando submetida à radiação ionizante visando seu uso em moldes planares para radioterapia de lesões cutâneas. Foram confeccionadas amostras de matrizes biopoliméricas de amido, constituído de água, amido, glicerina, duas variedades de açúcares na forma de pó, totalmente solúveis em água e produzida através da conversão do amido de milho. Durante o preparo das amostras variou-se as formulações para se obter melhor resistência da película plástica formada. As formações dos filmes de amido, foram realizadas através do método Casting com baixa viscosidade. As amostras foram submetidas à radiação em um acelerador linear clínico (linac) com feixes de raios-X de 10 MV e feixe de elétrons de 4 MeV, 6 MeV, 9 MeV e 12 MeV, e em uma fonte telecobaltoterapia com 1,25 MeV de energia. A dose total de radiação aplicada variou de 20 Gy a 140 Gy, um grupo controle foi mantido sem exposição à radiação. Após o processo de irradiação, foi realizada uma dosime... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Biopolymers can combine "in vivo" degradability with good biocompatibility and, in this way, can be used as encapsulating agents in order to release bioactive compounds. Within the group of biopolymers, polysaccharides have important advantages, such as high stability, safety, hydrophilicity and biodegradability. Polysaccharides has been object of studied, presenting promising results. This work aims to study the properties of biopolymers for use in pharmaceutical applications in the treatment of pathologies and to analyze the behavior of the biopolymer matrix based on starch when submitted to ionizing radiation for its use in planar models for radiotherapy of cutaneous lesions. An extensive bibliographic research was carried out aiming to understand the characteristics, properties and applications of the biopolymers in the health area, having as starch the base of the polymer matrices. Samples of biopolymeric were made matrices, consisting of water, starch, glycerin, two sugar varieties in the form of powder, totally soluble in water and produced through the conversion of corn starch were made. During the preparation of the samples the formulations were varied to obtain better resistance of the plastic film formed. The formations of the starch films were carried out using the casting method with low viscosity. The samples were subjected to radiation in a clinical linear accelerator (linac) with x-ray beams of 10 MV and electron beam of 4 MeV, 6 MeV, 9 MeV and 12 MeV, and in ... (Complete abstract click electronic access below) / Mestre

A preocupação com o meio ambiente tem gerado discussões em torno da sustentabilidade ambiental. Neste ponto, o design pode contribuir com o desenvolvimento de produtos voltados para a redução de impactos ambientais. Visando a colaboração do design com a preservação do meio ambiente, uma possível alternativa encontra- se na aplicação de materiais biodegradáveis em soluções de design. Dessa forma, esta pesquisa tem como objetivo geral investigar a viabilidade de aplicação dos compósitos biodegradáveis à base de biopolímero poli(butileno adipato-co-tereftalato e poli(ácido lático) com fibras de curauá no design de produto. Para isso, inicialmente foi realizado um mapeamento em torno de artigos que visam o desenvolvimento e a caracterização de compósitos biodegradáveis que utilizam fibras vegetais brasileiras como reforço. Este levantamento resultou em um panorama de pesquisas da área, os tipos de materiais que compõe os compósitos biodegradáveis, os tratamentos utilizados nas fibras e os aditivos na matriz, as formas de processamento e as propriedades mecânicas. A partir destas propriedades foi possível verificar os materiais que possuem propriedades semelhantes, e assim, averiguar em que tipo de produtos são empregados estes materiais, para posteriormente, examinar o emprego de compósitos biodegradáveis nestas aplicações. Desse modo, foi possível realizar o prognóstico de aplicações para os compósitos biodegradáveis. Na sequência do trabalho, realizou-se um estudo de caso em torno dos compósitos biodegradáveis, a fim de verificar a viabilidade de aplicação destes materiais. Utilizou-se a técnica de entrevistas semi estruturadas com designers de produto, que resultou na relação de atributos tangíveis, intangíveis com possíveis aplicações para os referidos compósitos. / Concern for the environment has generated discussions on environmental sustainability. At this poin, the design can contribute to the development of products designed to reduce environmental impacts. Aiming at the design collaboration with the preservation of the environment, a possible alternative is the use of biodegradable materials in design solutions. Thus, this research aims at investigating the feasibility of application of composite biopolymer based biodegradable poly (butylene adipate -co- terephthalate and poly (lactic acid) fiber curauá in product design. Initially we conducted a mapping around articles aimed at the development and characterization of biodegradable composites using Brazilian vegetable fibers as reinforcement. This survey resulted in an overview of research in the area, the types of materials that make up the biodegradable composites, the treatments used in the fibers and additives in matrix, forms processing and mechanical properties. based on these properties was possible to verify that the materials have similar properties, and thus determine what kind of products are used these materials to further examine the use of biodegradable composites in these applications. Thus, it was possible to perform the prediction of applications for biodegradable composites, following the study, there was a case study around biodegradable composites in order to verify the feasibility of these materials. to do this, we used the technique of semi-structured interviews with product designers, which resulted in the ratio of tangible attributes, intangible assets with potential applications for these composites.

Submitted by CLAUDIA VILALVA CASSARO null (claudia.v.cassaro@gmail.com) on 2018-03-21T13:51:17Z No. of bitstreams: 1 dissertacao claudia_cassaroFINAL.pdf: 3809022 bytes, checksum: a5c8891039ce6bc569b42ee66058c846 (MD5) / Rejected by Luciana Pizzani null (luciana@btu.unesp.br), reason: Solicitamos que realize uma nova submissão seguindo as orientações abaixo: Necessário fazer as seguintes correções no arquivo submetido: problema 1: Assinalar a agência de fomento (no seu caso CAPES). problema 2: No campo número do financiamento: colocar o número do processo. Assim que tiver efetuado as correções submeta o arquivo em PDF novamente. Agradecemos a compreensão. on 2018-03-22T12:35:28Z (GMT) / Submitted by CLAUDIA VILALVA CASSARO null (claudia.v.cassaro@gmail.com) on 2018-03-22T15:52:41Z No. of bitstreams: 1 dissertacao claudia_cassaroFINAL.pdf: 3809022 bytes, checksum: a5c8891039ce6bc569b42ee66058c846 (MD5) / Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2018-03-23T12:00:14Z (GMT) No. of bitstreams: 1 cassaro_cv_me_bot.pdf: 3809022 bytes, checksum: a5c8891039ce6bc569b42ee66058c846 (MD5) / Made available in DSpace on 2018-03-23T12:00:14Z (GMT). No. of bitstreams: 1 cassaro_cv_me_bot.pdf: 3809022 bytes, checksum: a5c8891039ce6bc569b42ee66058c846 (MD5) Previous issue date: 2018-02-23 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O reparo do tecido ósseo é um desafio antigo da ciência mundial. A partir das décadas de 70-80, os biomateriais passaram a ser explorados e hoje desempenham importante papel na engenharia tecidual. Estes, quando aplicados na enxertia óssea, devem ser bioativos, biocompatíveis e reabsorvíveis. A integração entre os diversos materiais de origem sintética e os arcabouços biológicos possui amplo espectro de uso. Dentre os arcabouços destacam-se os compostos à base de fibrina por suas propriedades hemostáticas, estabilidade e morfologia favorável à proliferação celular. A presente revisão abordou a estrutura e constituição do tecido ósseo e suas limitações no processo de reparo, a relevância da engenharia tecidual aplicada à esta finalidade e os biomateriais utilizados para otimizar tal processo, com ênfase especial aos materiais compostos por fosfatos de cálcio, células tronco mesenquimais e sua aplicação junto ao arcabouço biológico selante de fibrina, com destaque para o selante heterólogo de fibrina, agora denominado biopolímero de fibrina, um produto único e em fase de testes em experimentação animal e estudos clínicos. Propõe-se, ao final desta, uma nova abordagem para a regeneração de defeitos ósseos de ratos baseada na associação entre o selante heterólogo de fibrina, o fosfato de cálcio bifásico e células tronco mesenquimais. / Bone tissue repair is an ancient challenge of world science. From the 70s and 80s, biomaterials began to be explored and nowadays play an important role in tissue engineering. These, when applied in bone grafting, have to be bioactive, biocompatible and resorbable. The integration between the various materials of synthetic origin and biological scaffolds has a wide spectrum of use. Among these scaffolds, fibrin-based compounds are distinguished by their hemostatic properties, stability and morphology favorable to cell proliferation. The present review addressed the structure and constitution of bone tissue and its limitations in the repair process, the relevance of tissue engineering approaches applied to this purpose and the biomaterials used to optimize this process, with special emphasis on materials composed of calcium phosphates, mesenchymal stem cells and its application with the biological sealant fibrin scaffold, highlighting the heterologous fibrin sealant, now named as fibrin biopolymer, a unique product testing in animal experimentation and clinical studies. At the end of this, a new approach is proposed for the regeneration of rat bone defects based on the association between heterologous fibrin sealant, biphasic calcium phosphate and mesenchymal stem cells.