Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-04-28T14:17:05Z No. of bitstreams: 1 467672.pdf: 1155191 bytes, checksum: e28fce24bd1c2748fbcead34d7bf4c08 (MD5) / Made available in DSpace on 2015-04-28T14:17:05Z (GMT). No. of bitstreams: 1 467672.pdf: 1155191 bytes, checksum: e28fce24bd1c2748fbcead34d7bf4c08 (MD5) Previous issue date: 2015-01-23 / Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul - FAPERGS / The objectives of this in vitro study were: 1) to evaluate the surface roughness of a composite resin covered by sealing surface containing inorganic filler or not, subject to brushing; 2) to analyze, with the aid of scanning electron microscopy, the presence of the sealant after 48 months of simulated toothbrushing; 3) verify that the type of toothpaste reduces the useful life of sealants; 4) verify the presence of inorganic filler prevents the increase in roughness forward to brushing. For this, 54 samples were made of composite resin and divided into nine groups: G1 - without composite sealant (control); G2 - Sealant Fortify, toothpaste Colgate Professional Whitening; G3 - Fortify, Colgate Total Clean Mint; G4 - Fortify, Edel White Whitening; G5 - Fortify, Edel White Gum Care; G6 - Fortify Plus, Colgate Whitening Professional; G7 - Fortify Plus, Colgate Total Clean Mint; G8 - Fortify Plus White Whitening Edel; G9 - Fortify Plus, Edel White Gum Care and analyzed the simulated periods of brushing (12,24, 36 and 48 months). Reading roughness was performed at baseline and after each simulated toothbrushing period, using a surface roughness (SJ 201 Mututoyo).Two specimens of each group were observed by SEM. The data were submitted to ANOVA and Tukey's test (5%). There was a significant difference between the two sealants, with the sealant Fortify Plus rougher. Among the toothpaste, Colgate Clean Mint associated with Fortify Plus sealant obtained the higher roughness values. Lower values were associated with Gum Care Fortify sealant. The protective layer formed by the sealant remained partially by the end of 48 months of brushing, maintaining the smoothness of the composite. We conclude that both sealants guaranteed protection of the composite, and the Fortify Plus rougher for all assessed toothpastes. Clinical significance: The roughness of the surface sealant is changed for different toothpastes and different times of brushing and the addition of inorganic filler in sealants promotes different performances in relation to surface roughness. / Os objetivos deste estudo in vitro foram: 1) avaliar a rugosidade de superf?cie de uma resina composta coberta por selantes de superf?cie contendo carga inorg?nica ou n?o, submetidas ? escova??o simulada; 2) analisar, com aux?lio da microscopia eletr?nica de varredura, a presen?a dos selantes ap?s 48 meses de escova??o simulada; 3) verificar se o tipo de dentifr?cio diminui o tempo de vida ?til dos selantes; 4) verificar se a presen?a de carga inorg?nica previne o aumento da rugosidade frente ? escova??o. Para isto, foram confeccionadas 54 amostras de resina composta e divididas em nove grupos: G1 ? comp?sito sem selante (controle); G2 ? Selante Fortify, dentifr?cio Colgate Professional Whitening; G3 ? Fortify, Colgate Total Clean Mint; G4 ? Fortify, Edel White Branqueador; G5 ? Fortify, Edel White Gum Care; G6 ? Fortify Plus, Colgate Professional Whitening; G7 ? Fortify Plus, Colgate Total Clean Mint; G8 ? Fortify Plus Edel White Branqueador; G9 ? Fortify Plus, Edel White Gum Care e analisados nos per?odos simulados de escova??o de 12, 24, 36 e 48 meses. A leitura da rugosidade foi realizada no baseline e ap?s cada per?odo de escova??o simulada, utilizando um rugos?metro de superf?cie (SJ 201 Mututoyo). Dois corpos de prova de cada grupo foram observados em MEV.Os dados foram submetidos ? ANOVA e Teste de Tukey (5%). Verificou-se diferen?a significativa entre os dois selantes, sendo o selante Fortify Plus mais rugoso. Entre os dentifr?cios, o Colgate Clean Mint associado ao selante Fortify Plus obteve os maiores valores de rugosidade. Os menores valores observados foram com Gum Care associado ao selante Fortify. A camada de prote??o formada pelos selantes permaneceu parcialmente at? o final de 48 meses de escova??o, mantendo a lisura do comp?sito. Conclui-se que ambos os selantes garantiram prote??o da resina composta, sendo o Fortify Plus mais rugoso para todos os dentifr?cios avaliados. Signific?ncia cl?nica: A rugosidade dos selantes de superf?cie ? alterada em fun??o de diferentes dentifr?cios e diferentes tempos de escova??o e a adi??o de carga inorg?nica nos selantes promove desempenhos diferentes em rela??o ? rugosidade superficial.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-04-28T16:58:10Z No. of bitstreams: 1 467673.pdf: 3861422 bytes, checksum: 17e17a32c5200320863899c206b0dbea (MD5) / Made available in DSpace on 2015-04-28T16:58:10Z (GMT). No. of bitstreams: 1 467673.pdf: 3861422 bytes, checksum: 17e17a32c5200320863899c206b0dbea (MD5) Previous issue date: 2015-03-27 / Head and neck cancer (HNC) has been a worldwide public health problem. Squamous cell carcinomas account for about 80% of these neoplasms and the main treatment modalities are surgery, radiotherapy (RT), chemotherapy (CT) or a combination between them. RT acts locally destroying tumor cells. However, this therapy is not selective and also affects rapid renewal cell groups such as the salivary glands, oral mucosa and skin, resulting in a wide range of deleterious effects, being oral mucositis (OM) one of these. This condition is characterized by painful ulcerations that can progress to severe conditions that compromise the course of cancer treatment. The etiology of OM is oxidative stress (OS) generated by RT, that would be able to induce the production of reactive oxygen species (ROS) responsible for cellular damage and initiation of injuries. Antioxidants (AOX) are agents produced by humans or acquired by diet and vitamin supplements that eliminate ROS and minimize OS.The objective of this study was to assess the effect of 2 types of antioxidants, vitamin E (VE) and Aloe vera (AV) in prevention and management of radioinduced OM in a murine model by clinical and histological analysis. The animals were randomly divided into 3 groups of 12 animals each (400 mg VE, 70% AV and control) and 2 time periods (5 and 7 days). They were irradiated with a single dose of 30 Gy, and after 24h, a lesion was produced on the ventral tongue of each animal. The products were applied daily in their respective group until euthanasia. On clinical evaluation, it was observed the presence of inflammatory signs, presence or absence of induced ulcer and measurement of their size. Lesions were present more frequently in the control group animals in both periods of observation.The size of the ulcers was greater in the control group compared with the groups AV and VE (5 days: p = 0.006; 7 days: p = 0.002). Under microscopic analysis, the degree of inflammation differed between the study groups and experimental periods. At 5 days, the statistical difference was not significant amongst groups. However, after 7 day period, the animals in the control group displayed intense inflammation, while those in groups VE and AV exhibited mild to moderate inflammation (p = 0.002). Given the severity of OM injuries and their implications to the patient, it urges the search of alternative therapies to prevent or reduce clinical manifestations. The results of this study suggest that VE and AV may contribute to minimize inflammatory response and improve the healing of induced tongue lesions of rats submitted to radiation. / As neoplasias malignas de cabe?a e pesco?o (NMCP) representam um problema de sa?de p?blica mundial. Os carcinomas espinocelulares constituem cerca de 80% dessas neoplasias e tem como principais modalidades de tratamento a cirurgia, a radioterapia (RT) e a quimioterapia (QT) que podem ser utilizadas de forma isolada ou associadas. A RT age localmente destruindo as c?lulas tumorais, no entanto, n?o ? seletiva, atingindo tamb?m c?lulas saud?veis de r?pida renova??o como as das gl?ndulas salivares, mucosa oral e pele. Em consequ?ncia ao dano ?s c?lulas normais esta terapia gera efeitos delet?rios importantes como, por exemplo, a mucosite oral (MO). Esta condi??o caracteriza-se pela presen?a de ulcera??es dolorosas que podem evoluir para quadros t?o graves que comprometam o curso do tratamento oncol?gico. Associada a etiologia da MO est? o estresse oxidativo (EO) gerado pela RT, que seria capaz de induzir a produ??o de esp?cies reativas de oxig?nio (ROS) respons?veis pelo dano celular e inicia??o das les?es. Para limitar o EO existem subst?ncias chamadas antioxidantes (AOX), que tem como principal fun??o eliminar as ROS.Estas podem ser produzidas naturalmente pelo organismo e tamb?m adquiridas atrav?s da dieta e suplementos vitam?nicos. O objetivo deste estudo foi testar 2 tipos de antioxidantes, vitamina E(VE) e Aloe vera(AV), na preven??o e manejo da MO induzida por radia??o em modelo murino, atrav?s da an?lise cl?nica e histol?gica Os animais foram divididos randomicamente em 2 grupos com 12 animais cada (VE 400mg; AV 70%) e 1 grupo com 11 animais (controle) e em 2 tempos experimentais (5 e 7 dias). Irradiou-se cada grupo com dose ?nica de 30Gy e ap?s 24h produziu-se uma les?o ulcerada no ventre lingual de cada animal medindo 6mm de comprimento e 3mm de largura. Os produtos estudados foram aplicados diariamente em seu respectivo grupo at? a eutan?sia programada. Durante a avalia??o cl?nica, foram observados a presen?a de sinais inflamat?rios, presen?a ou aus?ncia da ?lcera induzida e mensurado o tamanho das mesmas. Nesta fase do experimento foi poss?vel constatar que as les?es foram mais frequentes nos animais dos grupos controle em ambos os tempos. O tamanho das ?lceras foi maior nos grupos controle em compara??o com os grupos VE e AV (5 dias: p=0,006; 7 dias: p=0,002).Na an?lise microsc?pica o grau de inflama??o diferiu tanto nos grupos de estudo quanto nos tempos experimentais. Em 5 dias a diferen?a entre os grupos n?o foi estatisticamente significante. J? em 7 dias os animais do grupo controle apresentaram inflama??o intensa, enquanto os que receberam VE e AV variaram entre leve e moderada (p=0,002). Diante da gravidade das les?es de MO e suas implica??es ao paciente, ? de suma import?ncia que se encontre alternativas terap?uticas para prevenir ou amenizar suas manifesta??es cl?nicas. Os resultados deste estudo sugerem que os AOX presentes na VE e no AV podem favorecer a redu??o da intensidade do processo inflamat?rio envolvido na MO, bem como a severidade das les?es.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-07T12:17:11Z No. of bitstreams: 1 468069 - Texto Completo.pdf: 1430495 bytes, checksum: 0bb6fedf792362692d6371829315b2e1 (MD5) / Made available in DSpace on 2015-05-07T12:17:11Z (GMT). No. of bitstreams: 1 468069 - Texto Completo.pdf: 1430495 bytes, checksum: 0bb6fedf792362692d6371829315b2e1 (MD5) Previous issue date: 2015-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Hyaluronic acid (HA) and polymethylmethacrylate (PMMA) are the most used dermal fillers nowadays. The indiscriminate use of such substances has brought to light unwanted adverse effects. Thus biocompatibility studies are a necessity, since the scientific literature does not clarify the etiology of these effects. The current research has evaluated cytotoxic, genotoxic and mutagenic responses from distinct in vitro tests performed in an independent manner. The cytotoxicity potential of the materials was evaluated by the induction of an inhibition halo in a solid yeast cultures. To conduce a preliminary view, the dilutions ranging from 10-2 to 10-5 were verified. For quantitative test, the colonies were counted to estimate the CFU/mL (colony-forming units per milliliter) values. Halo inhibition test showed that only silicone, used as a positive control, was capable of inducing cytotoxicity in this yeast. The preliminary experiment also indicated silicone and HA 16 mg/mL as a cellular toxicity inductor material. Quantitative test indicated that HA 20 mg/mL and 0.1mL volume inhibited cell proliferation (ANOVA, Tukey test, p? 0.05). PMMA was dosedependent to 2 and 10% concentrations (Tukey test, p? 0.05). 30% PMMA showed cell proliferation inhibition similar to the negative control. Silicone proved to inhibit S. cerevisiae cell proliferation (Tukey test, p? 0.05). In a second investigation, in Chinese hamster lung fibroblasts (V79 cells), the cytotoxic, genotoxic and mutagenic potentials of 20 mg/mL HA and 30% PMMA were determined. For testing these effects, clonogenic survival, comet and micronucleus assay were performed. HA and PMMA were able to decrease the colony formation when cultures were exposed to compounds by 24 h followed by 6 days in drug-free media. In addition, no genotoxic effects were detected in the 3 or 24 h of exposure to HA or PMMA. In the same manner, both dermal fillers did not induce increase in the micronucleus frequency in binucleated cells. Taken together, these results suggest that (1) 20 mg/mL HA and 10% PMMA are cytotoxicity inductors for the eukaryotic model S. cerevisiae; (2) 20 mg/mL HA and 30% PMMA have a weak cytotoxic activity in V79 cells; (3) the tested substances do not cause detectable DNA damage and chromosome alterations in V79 cells. / O ?cido hialur?nico (AH) e o polimetilmetacrilato (PMMA) s?o os materiais de preenchimento est?tico mais empregados na atualidade. Seu uso indiscriminado tem evidenciado efeitos indesej?veis nos usu?rios destes produtos, tornando necess?rio o estudo da biocompatibilidade dos mesmos, uma vez que a literatura cient?fica dispon?vel n?o esclarece, de forma conclusiva, a etiologia das rea??es adversas que podem se desenvolver a partir da sua inje??o. A presente pesquisa avaliou a resposta citot?xica, genot?xica e mutag?nica, a partir de testes in vitro distintos e efetuados de maneira independente. Utilizando a levedura Saccharomyces cerevisiae averiguou-se a citotoxicidade do AH (16 mg/mL e 20 mg/mL) e do PMMA (2%, 10% e 30%), por meio de experimentos qualitativos e quantitativos. O primeiro procedeu-se pela indu??o de halo de inibi??o. Para conduzir uma an?lise preliminar, as dilui??es 10-2 a 10-5 foram verificadas. No teste quantitativo, as col?nias formadas foram contadas em UFC/mL (unidades formadoras de col?nias por mililitro). Os dados observados em levedura demonstraram que no teste do halo de inibi??o, o silicone, utilizado como controle positivo, foi o ?nico material capaz de induzir citotoxicidade. O exame preliminar tamb?m indicou o silicone e o AH 16 mg/mL como indutores de toxicidade celular. Na an?lise quantitativa, o AH 20 mg/mL no volume de 0,1 mL inibiu a prolifera??o celular (ANOVA, teste de Tukey, p? 0,05). O PMMA apresentou resposta dose-dependente nas concentra??es de 2% e 10% (teste de Tukey, p? 0,05). Por outro lado, o PMMA 30% exibiu n?veis de crescimento celular semelhantes ao controle negativo. O silicone confirmou o impedimento de prolifera??o celular em S. cerevisiae (teste de Tukey, p? 0,05). Numa segunda investiga??o, em cultura de fibroblastos pulmonares de hamster Chin?s (linhagem V79), foram determinados os potenciais de citotoxicidade, genotoxicidade e mutagenicidade do AH 20 mg/mL e do PMMA 30%. Para esses par?metros, a abordagem envolveu os ensaios clonog?nico, cometa e micron?cleos. O AH e o PMMA foram capazes de diminuir o crescimento de col?nias quando as culturas foram expostas aos produtos por 24 h, seguidos por 6 dias em meio com aus?ncia das drogas. N?o foram detectados efeitos genot?xicos em 3 h ou 24 h de exposi??o ao AH ou PMMA. Da mesma maneira, ambas as subst?ncias n?o induziram aumento na frequ?ncia de micron?cleos em c?lulas binucleadas. Os resultados obtidos permitem sugerir que (1) o AH 20 mg/mL e o PMMA 10% s?o indutores de citotoxicidade em modelo eucarioto S. cerevisiae; (2) o AH e o PMMA possuem fraca citotoxicidade sobre a linhagem V79; (3) os materiais testados n?o provocam danos no DNA e altera??es cromoss?micas detect?veis.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-07T13:39:38Z No. of bitstreams: 1 468073 - Texto Completo.pdf: 2289187 bytes, checksum: e95cc5f55a1d9f82d97c26b1b5b34118 (MD5) / Made available in DSpace on 2015-05-07T13:39:38Z (GMT). No. of bitstreams: 1 468073 - Texto Completo.pdf: 2289187 bytes, checksum: e95cc5f55a1d9f82d97c26b1b5b34118 (MD5) Previous issue date: 2015-01-09 / Carcinogenesis is a complex multifactorial process dependent on inherent to individual or environmental factors. Environmental polycyclic aromatic hydrocarbons (PAHs) have been pointed as a risk factor for different types of cancer, especially in upper aerodigestive tract. Chimarr?o is a hot mat? beverage containing PAHs that are incorporated to the herb during drying process of the leaves. Individuals that drink chimarr?o are exposed do PAHs, which can be assessed by 1-hydroxypyrene-glucuronide levels (1-OHPG). The aim of the present study was to evaluate cytomorphometric alterations of oral mucosa epithelium in chimarr?o drinkers correlating them to salivary and urinary levels of 1-OHPG. Adult males and females without history of regular alcohol use were allocated into 4 groups: (1)=39 chimarr?o drinkers who did not smoke; (2)=25 chimarr?o drinkers who smoked; (3)=27 smokers who did not drink chimarr?o; and (4)=27 individuals who had neither of these habits. Mucosal scrapings were performed and subjected to qualitative (Papanicolaou) and quantitative (nuclear area, cytoplasmic area, nucleus/cytoplasm ratio) analysis. Urine and saliva samples were assayed for 1-OHPG by high-performance liquid chromatography (HPLC). All samples were classified into Papanicolaou class I. Nuclear and cytoplasmic areas of epithelial cells in soft palate smears did not significantly differ between the groups, whereas in buccal (cheek) mucosa they were significantly greater in the chimarr?o group than in controls. The nucleus/cytoplasm ratio as well as salivary and urinary concentrations of 1-OHPG did not significantly differ. Urinary and salivary 1-OHPG concentrations were positively correlated to each other but they did not show any correlation with the cytometric variables. Nuclear and cytoplasmic areas were positively correlated to each other in either palate or buccal mucosa smears. Conclusion : Chimarr?o was associated with neither cytomorphometric alterations in epithelial cells of palate smears nor urinary and salivary 1-OHPG levels. Buccal smears showed higher nuclear and cytoplasmic area in the chimarr?o group, but this result does not support an association with dysplasia. / A carcinog?nese ? um processo complexo e multifatorial dependente de fatores que s?o inerentes ao indiv?duo ou ambientais. A presen?a de hidrocarbonetos arom?ticos polic?clicos (HAPs) no meio ambiente tem sido apontada como um dos fatores de risco para o desenvolvimento de diferentes tipos de c?ncer, principalmente do trato aerodigestivo superior. O chimarr?o ? uma bebida quente ? base de erva-mate que concentra HAPs incorporados ? erva durante o processamento de secagem das folhas. Indiv?duos com o h?bito de tomar chimarr?o est?o expostos aos HAPs, exposi??o esta que pode ser mensurada por meio da quantifica??o de 1-hidroxipireno glucoron?deo (1-OHPG). O presente estudo teve por objetivo investigar altera??es citomorfom?tricas do epit?lio da mucosa oral em tomadores de chimarr?o e correlacion?-las com os n?veis salivares e urin?rios de 1-OHPG. Indiv?duos adultos, de ambos os sexos e sem hist?ria de uso regular de ?lcool, foram distribu?dos em quatro grupos: (1)=39 indiv?duos tomadores de chimarr?o, n?o-fumantes; (2)=25 fumantes tomadores de chimarr?o, (3)=27 indiv?duos fumantes n?o tomadores de chimarr?o e (4)=27 indiv?duos sem nenhum dos h?bitos supracitados (grupo?controle). Amostras de citologia exfoliativa foram obtidas da mucosa do palato mole e mucosa jugal para avalia??o qualitativa (Papanicolaou) e quantitativa (?rea nuclear, ?rea citoplasm?tica e propor??o n?cleo/citoplasma). Amostras de saliva e urina foram coletadas para dosagem de 1-OHPG por meio de cromatografia l?quida de alta performance (HPLC). Os resultados evidenciaram todas as amostras citol?gicas classificadas como classe I de Papanicolaou. As ?reas nuclear e citoplasm?tica das c?lulas epiteliais do palato, bem como a propor??o n?cleo/citoplasma de ambos os s?tios n?o diferiram significativamente entre os grupos. No grupo chimarr?o, as c?lulas epiteliais da mucosa jugal exibiram ?rea nuclear e ?rea citoplasm?tica significativamente maiores que o grupo-controle. Foi observada correla??o positiva entre ?rea nuclear e citoplasm?tica, bem como entre concentra??es salivares e urin?rias de 1-OHPG. Conclus?o : N?o foi observada associa??o do chimarr?o com altera??es citomorfom?tricas das c?lulas epiteliais do palato, ou com os n?veis salivares e urin?rios de 1-OHPG. Embora ?rea nuclear e citoplasm?tica tenham sido significativamente maiores no grupo chimarr?o do que no controle, o presente estudo n?o permite inferir que essas altera??es tenham tend?ncia displ?sica.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-14T20:09:46Z No. of bitstreams: 1 468627 - Texto Completo.pdf: 868809 bytes, checksum: da322f4444f2d9e11e6d453c3af66303 (MD5) / Made available in DSpace on 2015-05-14T20:09:46Z (GMT). No. of bitstreams: 1 468627 - Texto Completo.pdf: 868809 bytes, checksum: da322f4444f2d9e11e6d453c3af66303 (MD5) Previous issue date: 2015-03-12 / Tooth movement in response to orthodontic forces is results from remodeling changes that affect the periodontal ligament and the bone surrounding teeth. This movement may be faster or slower depending on the physical characteristics of the force applied and the biological response induced, and is characterized by zones of compression and tension on the periodontal ligament. Knowledge about the tissue changes generated in this process is essential to plan the movement of teeth and their adjoining structures into a new position without any damage to tissues. In this study, the literature was reviewed to improve our understanding of the cellular, biochemical and molecular phenomena that affect the structures of the periodontal ligament (PL), as well as the alveolar bone surrounding the tooth and involved in the tooth movement that is induced in the process. The purpose of our systematic review was to evaluate studies about cytokines in gingival fluid (GF) during orthodontic treatment, summarize the patterns of cytokine regulation that have been more frequently studied and discuss their clinical implications. Additionally, we investigated changes in immunological and bone modulators in response to the application of orthodontic forces. For that purpose, GF of teeth that were involved in maxillary expansion was evaluated qualitatively to measure interleukin concentrations and their predictive value as a market of inflammatory response intensity and bone turnover during orthodontic movement. The purpose of this investigation was to evaluate whether the force applied on the PL during maxillary expansion affected the composition of the gingival crevicular fluid (GCF), specifically the level of IL-17 on the sides where tension and compression were applied. GCF samples were collected from the mesiobuccal and mesiolingual areas of 32 molars of 16 patients aged 7 to 14 years who had to undergo maxillary expansion. These patients started orthodontic treatment sometime between 2012 and 2013 at the School of Dentistry of the Pontif?cia Universidade Cat?lica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil. Metal bands were placed around the permanent maxillary first molars to hold a modified Hyrax expander. One of the investigators collected clinical data about the sites listed above during activation of the expander. An enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin levels. The sites were examined to measure IL-17 before the device was placed and at 1, 7 and 14 days of active use of the expander. The forces applied resulted in an increase in IL-17 expression in periodontal tissues one day after the application of tension and compression at both measurement sites. IL-17 levels tended to be higher (p ? 0.05) seven days after the Hyrax screw was activated. From that time to the fourteenth day, there was a tendency to a decrease in IL-17 levels (p ? 0.05). We concluded that there was an increase in IL-17 expression at both tension and compression sites in response to force application. / O movimento dent?rio promovido por for?as ortod?nticas ? baseado em altera??es remodeladoras que ocorrem no ligamento periodontal e no osso que circundam os dentes. Este movimento pode ocorrer de forma mais r?pida ou lenta, dependendo das caracter?sticas f?sicas da for?a aplicada e da resposta biol?gica induzida e ? caracterizado por cria??o de zonas de compress?o e tens?o no ligamento periodontal. O conhecimento das altera??es teciduais geradas ? essencial para o planejamento do movimento do dente e de suas estruturas anexas para uma nova posi??o, sem danos aos tecidos. Neste estudo, uma revis?o de literatura teve como objetivo possibilitar a compreens?o dos fen?menos celulares, bioqu?micos e moleculares que ocorrem nas estruturas do ligamento periodontal (PDL) e, tamb?m do osso alveolar ao redor do dente e que est?o envolvidos na movimenta??o dent?ria induzida. A revis?o sistem?tica teve como objetivo avaliar estudos sobre citocinas no fluido gengival (GCF) durante o tratamento ortod?ntico, resumindo os padr?es de regula??o das citocinas mais estudadas e explorar suas implica??es cl?nicas. Al?m disso, foram investigadas altera??es dos moduladores imunol?gicos e ?sseos em resposta a aplica??o da for?a ortod?ntica. Para tanto o fluido gengival de dentes submetidos ao procedimento de expans?o maxilar foi avaliado qualitativamente verificando concentra??es de interleucinas e seu valor preditivo como marcador da intensidade da resposta inflamat?ria e turnover ?sseo na movimenta??o ortod?ntica. O objetivo da investiga??o foi avaliar se a for?a aplicada sobre o ligamento periodontal durante a expans?o maxilar se reflete na composi??o do Fluido do Sulco Gengival (FSG), mais especificamente no n?vel de interleucina IL-17 dos lados de tens?o ou compress?o. As amostras do FSG foram coletadas das regi?es mesiovestibulares e m?siopalatinas de 32 molares de 16 pacientes com idade entre 7 a 14 anos, que tinham necessidade de expans?o maxilar. Estes pacientes iniciaram o tratamento ortod?ntico durante o per?odo compreendido entre 2012 e 2013, na Faculdade de Odontologia da Pontif?cia Universidade Cat?lica do Rio Grande do Sul (PUCRS). Eles receberam an?is nos primeiro molares permanentes superiores para a confec??o de um aparelho disjuntor de Hyrax modificado. Os dados cl?nicos foram coletados por um investigador nas regi?es supracitadas durante o per?odo de ativa??o do aparelho. Empregando-se o ensaio imunoenzim?tico (ELISA) foram detectados os n?veis da interleucina. Os locais foram analisados para IL-17 antes da coloca??o do aparelho, 1, 7 e 14 dias de uso ativo do aparelho. As for?as empregadas resultaram em um aumento da express?o de IL-17 nos tecidos periodontais ap?s um dia de aplica??o em ambos os lados, de tens?o e compress?o. Os n?veis de IL-17 tenderam a ser maiores (p ? 0,05) ap?s sete dias de ativa??o do parafuso. Deste momento, at? o d?cimo quarto dia, observou-se uma tend?ncia para diminui??o dos n?veis de IL-17 (p ? 0,05). Concluiu-se que houve um aumento na express?o de IL-17 em ambos os locais, de tens?o e compress?o, em resposta ? aplica??o de for?a.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-14T20:32:00Z No. of bitstreams: 1 468626 - Texto Completo.pdf: 36893570 bytes, checksum: f40ac0cf663ae01a836d6017460f63fc (MD5) / Made available in DSpace on 2015-05-14T20:32:00Z (GMT). No. of bitstreams: 1 468626 - Texto Completo.pdf: 36893570 bytes, checksum: f40ac0cf663ae01a836d6017460f63fc (MD5) Previous issue date: 2015-03-27 / Objective: to evaluate the stability of PLGA 50:50 formulations incorporated with recombinant human growth hormone (rhGH) under different storage time and temperatures. Material and Methods: a total of 27 macrospheres of PLGA + rhGH were prepared using 7.5g of PLGA and 1,33mg of rhGH. Twenty-four of the 27 samples were equally divided into 4 groups (I, II, III and IV), according to the storage time, and two subgroups (A and F), according to the storage temperature prior to degradation. The remaining 3 samples were degraded immediately after its preparation (group 0). The concentration of rhGH released from the matrices was measured by UV-visible spectrophotometry absorption analysis. A calibration curve defined rhGH concentration values based on the final absorbance values. All colected data were submitted to descriptive and analytical statistical analysis with a confidence interval of 95% (p <0.05). Results: in general, the samples stored for different periods of time followed similar rhGH release rate of the samples belonged to group 0, with peaks of major and minor release rates and showing an important release until the 14th day of degradation, but no statistically significant differences between the study groups. Although higher absorbance values were constantly found for the matrices in subgroup F, the pattern of rhGH release was similar and no statistically significant difference was evident between the subgroups compared. The pH analysis revealed a lower pH for the matrices stored refrigerated, with no statistical difference. Conclusion: PLGA + rhGH formulations are stable. Until the 28th day, storage time and temperature do not affect the stability of the formulation. / Objetivo: avaliar a estabilidade das formula??es de PLGA 50:50 incorporadas com horm?nio de crescimento recombinante humano (rhGH) sob diferentes per?odos e temperaturas de armazenamento. Materiais e m?todos: um total 27 matrizes de PLGA + rhGH foram confeccionadas utilizando 7,5g de PLGA e e 1,33mg de rhGH. Vinte e quatro das 27 matrizes foram divididas igualmente em 4 grupos (I, II, III e IV), de acordo com o tempo de armazenamento, e em 2 subgrupos (A e F), de acordo com a temperatura de armazenamento previamente ? sua degrada??o. As 3 matrizes restantes foram degradas imediatamente ap?s sua confec??o (grupo 0). A concentra??o de rhGH liberado das matrizes foi mensurada em dias determinados atrav?s da an?lise de espectrofotometria de absor??o UV-vis?vel do sobrenadante removido do tubos de ensaio onde as matrizes foram degradadas. Uma curva de calibra??o definiu os valores de concentra??o de rhGH baseado nos valores finais de absorb?ncia. Os dados obtidos foram submetidos ? an?lises estat?sticas descritivas e anal?ticas com intervalo de confian?a de 95% (p<0,05). Resultados: em geral, as matrizes armazenadas por diferentes per?odos de tempo seguiram o mesmo padr?o de libera??o de rhGH das matrizes do grupo 0, com picos de maior e menor libera??o entre os dias avaliados e demonstrando uma libera??o expressiva at? o 14o dia de degrada??o, mas sem diferen?as estatisticamente significativas entre grupos do estudo. Apesar de constantemente haver maiores valores de absorb?ncia para as matrizes do subgrupo F, o padr?o de libera??o de rhGH foi similar para os dois subgrupos, e n?o houve diferen?a estatisticamente significante entre os dois subgrupos comparados. A an?lise de pH revelou menor pH para as matrizes armazenadas em ambiente refrigerado, sem diferen?a estat?stica. Conclus?o: as formula??es de PLGA incorporadas com rhGH s?o est?veis. At? 28 dias, o tempo e a temperatura de armazenamento n?o influenciam na estabilidade da formula??o.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-14T20:53:10Z No. of bitstreams: 1 468625 - Texto Completo.pdf: 2863238 bytes, checksum: 3e057c78a6b6e5cfbd0cf6767d59dd26 (MD5) / Made available in DSpace on 2015-05-14T20:53:10Z (GMT). No. of bitstreams: 1 468625 - Texto Completo.pdf: 2863238 bytes, checksum: 3e057c78a6b6e5cfbd0cf6767d59dd26 (MD5) Previous issue date: 2014-03-26 / The clinical success of the implant treatment is strongly related with the achievement and maintenance of the stability of the fixture. As the surface topography, roughness and wettability are modifications that can affect the quality of the osseointegration, several studies have analyzed the benefits of these modifications. The histomorphometric analyses of the bone-to-implant contact (BIC) and the resonance frequency analyses (RFA) are commonly used to verify the osseointegration and implant stability. In the present study, 32 implants with two different surfaces treatment were installed in the tibia of 16 New Zealand rabbits and splitted in two groups. The Group I was formed by Neodent Drive implants, with sandblasted, acid etched and hydrophilic surface (Neodent Drive Acqua, Neodent, Curitiba, Brasil). The Group II was formed by Neodent Drive implants, with sandblasted and acid etched surface (Neodent Drive Porous, Neodent, Curitiba, Brasil). The samples were analyzed at 07, 14, 30 and 60 days after the implant placement. The results showed statistically significant differences between the mean values for the BIC at 14 (p=0.028) and 30 days (p=0.028). The mean BIC for the Acqua implants (Group I) were 43,32 ? 1,31% and 55,03 ? 5,14%, at 14 and 30 days respectively. The mean BIC in the Porous implants (Group II) were 46,27 ? 0,10% and 47,13 ? 0,67%, at 14 and 30 days, respectively. At 60 days, the mean BIC for the Acqua implants were 54,68 ? 3,51% and for the Porous implants were 50,86 ? 1,03%. There were not statistically significant differences in the implant stability quotient (ISQ) measurements between Acqua and Porous implants, except at 07 days after the implant placement. At day 07, the results of the present study showed higher ISQ values for the Porous implants (68,75 ? 4,16%) than Acqua implants (61,5 ? 8,01%). The follow-up ISQ analyses in the same Group, showed statistically significant increases of the mean values from 7 to 60 days and from 30 to 60 days, for both groups. In conclusion, the sandblasted and acid etched treatment used to create roughness on the Neodent implant surface is reliable and allows bone apposition, as the BIC and ISQ values found were similar or superior than the showed in the literature for similar animal models and similar implant surface. The increase of the BIC and ISQ values on the follow up showed the achievement of the secondary stability and the increase in stiffness between the implant fixture and the surrounding tissues. There were not identified better results with the hydrophilic surface during the early period of healing. Thus, further investigations are necessary to confirm the suggested results in this present study. / A obten??o e a manuten??o da estabilidade do implante s?o pr?-requisitos para o sucesso cl?nico das pr?teses sobre implantes. Visto que a topografia, a rugosidade e a hidrofilicidade s?o algumas das altera??es da superf?cie dos implante que podem alterar a resposta do tecido ?sseo, diversos estudos tem procurado entender os benef?cios que estas modifica??es podem proporcionar. A quantifica??o histomorfom?trica do contato osso-implante (BIC) e a an?lise de frequ?ncia de resson?ncia (AFR) s?o par?metros frequentemente utilizados na implantodontia para verificar a osseointegra??o e a estabilidade dos implantes. No presente estudo, foram instalados 32 implantes com duas diferentes superf?cies nas t?bias de 16 coelhos, divididos em dois grupos: Grupo I) constitu?do pelos implantes com superf?cie tratada por jateamento de areia, ataque ?cido, hidrof?lica e modificada quimicamente (Neodent Drive? Acqua, Neodent, Curitiba, Brasil) e Grupo II) constitu?do pelos implantes com superf?cie tratada por jateamento de areia e ataque ?cido (Neodent Drive? Porous, Neodent, Curitiba, Brasil). As amostras foram analisadas ap?s 7, 14, 30 e 60 dias da coloca??o dos implantes. Na compara??o da m?dia geral do BIC, entre Acqua e Porous, foram observadas diferen?as estatisticamente significantes no per?odo de 14 dias (p=0,028) e no per?odo de 30 dias (p=0,028). As medidas m?dias de BIC obtidas para os implantes Acqua foram 43,32 ? 1,31% e 55,03 ? 5,14%, aos 14 e 30 dias respectivamente. Para os implantes Porous, as medidas de BIC foram 46,27 ? 0,10% e 47,13 ? 0,67%, aos 14 e 30 dias respectivamente. No per?odo de 60 dias, os valores m?dios de BIC da superf?cie Acqua foram 54,68 ? 3,51% e da superf?cie Porous foram 50,86 ? 1,03%. Na compara??o da m?dia geral do quociente de estabilidade do implante (QEI), entre Acqua e Porous, n?o foram observadas diferen?as estatisticamente significantes entre os grupos, com exce??o no per?odo de 07 dias, no qual os resultados do presente estudo demonstraram maiores valores de QEI para o grupo Porous (68,75 ? 4,16%) do que para o grupo Acqua (61,5 ? 8,01%). A an?lise do QEI durante os diferentes per?odos dentro de um mesmo grupo, demonstrou diferen?as estatisticamente significativas entre os per?odos de 7 vs 60 dias e 30 vs 60 dias, para a m?dia geral do QEI no grupo Acqua e no grupo dos implantes Porous. As conclus?es do presente estudo s?o que os resultados demonstraram que a modifica??o da superf?cie por jateamento de areia e ataque ?cido utilizada para produzir as superf?cies Acqua e Porous resulta em uma adequada aposi??o ?ssea, visto que os valores do BIC e QEI encontrados s?o similares ou superiores aos resultados encontrados na literatura com modelos experimentais e superf?cies similares. Os aumentos dos valores do BIC e QEI durante os per?odos p?s-operat?rios revelaram a obten??o de estabilidade secund?ria e maior rigidez na ancoragem do implante com o passar do tempo. N?o foram identificados melhores resultados com a superf?cie hidrof?lica nos per?odos iniciais da osseointegra??o. Sendo assim, s?o necess?rios estudos pr?-cl?nicos e cl?nicos para confirmar as sugest?es que os resultados deste presente estudo revelaram.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-21T20:19:16Z No. of bitstreams: 1 469176 - Texto Completo.pdf: 1075538 bytes, checksum: c372985d28935427b18fce7ee072399d (MD5) / Made available in DSpace on 2015-05-21T20:19:16Z (GMT). No. of bitstreams: 1 469176 - Texto Completo.pdf: 1075538 bytes, checksum: c372985d28935427b18fce7ee072399d (MD5) Previous issue date: 2015-03-30 / Imaging exams performed in areas with the presence of metallic materials such as dental implants can decrease the accuracy of the exams due to metallic artifacts production generated in the peri-implant region. A sample of 21 dental implants placed in 10 patients with Branemark protocol prostheses was evaluated in the periimplant area in image exams of the post-surgery control using periapical radiography (paralleling technique), panoramic radiography and cone-beam computed tomography (CBCT). The radiographic density of different areas bounded near the dental implants were calculated and compared considering their locations and the imaging exams using the software ImageJ. Regarding the different imaging exams, the CBCT showed significant differences between the measured areas (p<0.001), while in periapical (p=0.430) and panoramic (p=0.149) radiographs did not occur significant difference between the gray mean values in these areas. Comparing the areas in the three exams it was observed larger values next to the implants in all exams: CBCT (126.20 and 127.44) panoramic radiography (106.51 and 106.09) and periapical radiography (120.32). The more distant areas and the areas closest to neighboring implants had significant different values in the two reconstructed images of CBCT when compared to the panoramic and periapical radiographs. The interference of dental implants in acquiring radiographic and tomographic exams was confirmed by altered radiographic density mean values in the peri-implant area. Comparing the three exams it was noticed that the CBCT image showed the most significant interference of the dental implants. / Os exames por imagem realizados em ?reas que apresentam materiais met?licos, como implantes dent?rios, podem ter sua qualidade prejudicada devido ? produ??o de artefatos met?licos gerados na regi?o peri-implantar. Uma amostra de 21 implantes dent?rios, em 10 pacientes com pr?teses do tipo protocolo Branemark, foi avaliada com o objetivo de comparar as densidades radiogr?ficas na regi?o periimplantar em exames de imagens de controle p?s-operat?rio com radiografia periapical (t?cnica do paralelismo), radiografia panor?mica e tomografia computadorizada de feixe c?nico. Os valores m?dios de densidade em ?reas delimitadas pr?ximas aos implantes dent?rios foram calculados e comparados, de acordo com suas localiza??es, e entre os exames de imagens utilizando o programa ImageJ. Os exames de Tomografia Computadorizada de Feixe C?nico (TCFC) apresentaram diferen?as significativas entre as ?reas medidas (p<0,001), enquanto que nas radiografias periapical (p=0,430) e panor?mica (p=0,149) n?o houve diferen?a significativa entre os valores m?dios de densidade nas mesmas ?reas. Na compara??o entre as ?reas dos tr?s exames, observou-se valores maiores naquelas pr?ximas aos implantes em todos os exames: TCFC (127,88 e 120,71), panor?mica (106,51 e 106,09) e periapical (120,32). As ?reas mais distantes e pr?ximas aos implantes vizinhos mostraram valores significativamente diferentes nos dois cortes da TCFC quando comparados aos exames periapical e panor?mico. Sendo assim, foi poss?vel confirmar a interfer?ncia dos implantes dent?rios na obten??o dos exames radiogr?ficos e tomogr?ficos, pela altera??o dos valores m?dios de densidade radiogr?fica na regi?o peri-implantar. Na compara??o entre os tr?s exames de imagem investigados, verificou-se que as imagens de TCFC sofreram a maior interfer?ncia dos implantes dent?rios, quando comparadas com as imagens radiogr?ficas.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-21T20:48:49Z No. of bitstreams: 1 469173 - Texto Completo.pdf: 1264302 bytes, checksum: e68c7c79947a5c2f21eab8eb8bfce80d (MD5) / Made available in DSpace on 2015-05-21T20:48:49Z (GMT). No. of bitstreams: 1 469173 - Texto Completo.pdf: 1264302 bytes, checksum: e68c7c79947a5c2f21eab8eb8bfce80d (MD5) Previous issue date: 2015-03-25 / Head and neck radiotherapy often involves major salivary glands and causes morphologic and functional alterations, resulting in hyposalivation and xerostomia. Literature was reviewed in the first manuscript, addressing the structural changes observed in the salivary glands resulting from oxidative stress caused by radiotherapy and pathogenic mechanisms involved. Preventive and regenerative therapies for altered acinar morphology and glandular function were also discussed. Among the acute and late microscopic alterations observed in glandular tissue, there are particularly changes indicative of cell death, hypovascularization, formation of fibrous tissue and edema. Considering the evidences before mentioned, the aim of this study was to evaluate the effect of low level laser therapy (LLLT) on radiotherapy-induced morphological changes and immunodetection of caspase-3 protein in parotids of mice. Forty-one Swiss mice were divided into a control group and three experimental groups: radiotherapy, 2 J laser and 4 J laser. The experimental groups were exposed to ionizing radiation in a single session of 10 Gy. In the laser groups, a GaAlAs laser (830 nm, 100 mW, 0.028 cm2, 3.57 W/cm2) was used on the region corresponding to the parotid glands, with 2 J energy (20 sec, 71 J/cm2) or 4 J (40 sec, 135 J/cm2) per point. The animals were euthanized 48 hours or seven days after radiotherapy and parotid glands were dissected for morphological analysis and immunodetection of caspase-3. There was no significant difference between groups in the immunodetection of caspase-3, but the laser groups had a lower percentage compared to the radiotherapy group. Furthermore, the results indicated that LLLT promoted the preservation of acinar structure, reduced the occurrence of cytoplasmic vacuolation and stimulated parotid gland vascularization. Of the two LLLT protocols, the one using 4 J of energy showed better results. Given the methodological limitations of this study, further researches should be conducted in irradiated animals, using different LLLT protocols and observing glandular response, not only in the short term but also long term, when the occurrence of late changes in the salivary glands can be analyzed. / A radioterapia direcionada ? regi?o de cabe?a e pesco?o frequentemente envolve as gl?ndulas salivares maiores, as quais sofrem altera??es morfol?gicas e funcionais, resultando em hipossaliva??o e xerostomia. No primeiro artigo desta disserta??o foi realizada uma revis?o da literatura com o objetivo de abordar as altera??es estruturais observadas nas gl?ndulas salivares e os poss?veis mecanismos patog?nicos pelos quais o estresse oxidativo, decorrente da radioterapia, causa disfun??es salivares. Al?m disso, foram revisados os m?todos de preven??o e regenera??o da morfologia acinar e da fun??o glandular. Entre as altera??es microsc?picas agudas e tardias observadas no tecido glandular irradiado, podem-se citar altera??es indicativas de morte celular como a apoptose, hipovasculariza??o, forma??o de tecido fibroso e edema. Considerando as evid?ncias anteriormente mencionadas, o objetivo deste estudo foi avaliar, em gl?ndulas par?tidas de camundongos, o efeito da terapia laser de baixa pot?ncia (TLBP) sobre altera??es morfol?gicas causadas pela radioterapia e na imunodetec??o da prote?na caspase-3. Quarenta e um camundongos Swiss foram distribu?dos em um grupo controle e tr?s grupos experimentais: radioterapia, laser 2 J e laser 4 J. Os grupos experimentais foram submetidos ? radia??o ionizante em sess?o ?nica de 10 Gy. Nos grupos laser, um laser de diodo, GaAlAs (830 nm, 100 mW, 0,028 cm2, 3,57 W/cm2) foi utilizado de forma pontual sobre a regi?o correspondente ?s gl?ndulas par?tidas, com energia de 2 J (20 seg, 71 J/cm2) ou 4 J (40 seg, 135 J/cm2) por ponto. Os animais foram eutanasiados 48 h ou sete dias ap?s a radioterapia e as gl?ndulas par?tidas dissecadas para an?lise morfol?gica e imunodetec??o da caspase-3. N?o houve diferen?a significativa entre os grupos na imunodetec??o da caspase-3, entretanto, os grupos laser apresentaram percentuais inferiores aos do grupo radioterapia. Al?m disso, os resultados indicaram que a TLBP promoveu preserva??o da estrutura acinar, reduziu a ocorr?ncia de vacuoliza??o citoplasm?tica e estimulou a vasculariza??o glandular. Entre os protocolos de TLBP, o que utilizou a energia de 4 J apresentou os melhores resultados. Tendo em vista as limita??es metodol?gicas desta pesquisa, mais estudos devem ser conduzidos em animais irradiados, utilizando diferentes protocolos de TLPB e observando a resposta glandular, n?o apenas em curto prazo, como tamb?m em longo prazo, quando a ocorr?ncia de altera??es tardias nas gl?ndulas salivares pode ser analisada.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-22T11:14:29Z No. of bitstreams: 1 469172 - Texto Completo.pdf: 823400 bytes, checksum: 095e93ad0abe67b38883346b9108692f (MD5) / Made available in DSpace on 2015-05-22T11:14:29Z (GMT). No. of bitstreams: 1 469172 - Texto Completo.pdf: 823400 bytes, checksum: 095e93ad0abe67b38883346b9108692f (MD5) Previous issue date: 2015-03-25 / Introduction: the objective of this study was to evaluate the temperature variation of the pulp chamber during brackets bonding in different teeth types, with and without primer and in healthy or filled teeth. Materials and Methods: ninety human permanent teeth were used, 30 mandibular incisors, 30 maxillary bicuspid and 30 mandibular third molars, which were divided into 9 groups according to bonding technique (with or without light-curing primer), tooth type, filled or healthy tooth and thickness. A K-type thermocouple connected in a digital thermometer was introduced into the pulp chamber. Periapical radiographs were taken to check the thermocouple positioning and the brackets were bonded using a LED. The initial and maximum temperature reached during light curing were used to calculate the temperature variation. Results: the ANCOVA analysis showed that the bonding technique and tooth type (p?0.05) influenced the temperature variation. There was a greater increase in temperature using primer. Regarding the tooth type, the incisors experienced a major temperature increase, followed by molars and bicuspids. There was no statistically significant difference for healthy or filled teeth groups and thickness factor. Conclusion: a molar showed the lowest temperature variation with 0.2?C and a lower incisor with the greatest variation of 4.3?C. The bonding technique using primer and adhesive caused a greater temperature range compared to the bonding with adhesive only. The mandibular incisor obtained a greater temperature range, then the molar and bicuspid. Therefore, the brackets bonding didn`t exceed the 5,5?C recommended for the pulp health maintenance. Key words: light curing of dental adhesives, thermometers, braces, hot temperature, orthodontics. / ntrodu??o: o objetivo desse estudo foi avaliar a varia??o de temperatura da c?mara pulpar durante a colagem de br?quetes em diferentes tipos de dentes, com e sem utiliza??o de primer e em dentes h?gidos e restaurados. Materiais e M?todos: foram utilizados 90 dentes permanentes humanos, sendo 30 incisivos inferiores, 30 primeiros pr?-molares superiores e 30 terceiros molares inferiores, divididos em 9 grupos de acordo com a t?cnica de colagem, com ou sem primer, tipo de dente e se o dente era h?gido ou restaurado. Um termopar tipo K foi introduzido na c?mara pulpar e conectado ? um term?metro digital. Radiografias periapicais foram realizadas para conferir o posicionamento do termopar no interior da c?mara pulpar e os br?quetes foram colados utilizando um LED. As temperaturas inicial e m?xima atingida durante a fotopolimeriza??o foram utilizadas para calcular a varia??o da temperatura. Resultados: a an?lise de ANCOVA mostrou que a varia??o de temperatura foi influenciada pela t?cnica de colagem e pelo tipo de dente (p?0,05). Utilizando primer houve um maior aumento de temperatura em rela??o ao grupo sem primer. Quanto ao tipo de dente, os incisivos sofreram um maior aumento de temperatura, seguido dos molares e pr?-molares. N?o houve diferen?a estatisticamente significativa para os grupos de dentes h?gidos ou restaurados e para o fator espessura. Conclus?o: o dente que obteve menor varia??o de temperatura foi um molar com 0,2?C e, um incisivo inferior, com a maior varia??o, de 4,3?C. A t?cnica de colagem utilizando primer e adesivo causou uma varia??o de temperatura maior se comparado ? colagem apenas do adesivo. O incisivo inferior obteve uma maior varia??o de temperatura, seguida do molar e pr?-molar. Portanto, a colagem de br?quetes n?o excedeu os 5,5?C preconizados para manuten??o da sa?de pulpar.