In the first phase of this study, the diversity of methanogenic bacteria in avian ceca was found to be minimal. Based on 16S rDNA clone libraries, a common phylotype, designated CH101, ranged between 92.86 to 100 % of the total clones whereas less than 1% of the other phylotypes were found. On the basis of the sequence identity, all of the sequences, except sequence CH1270, are related from 98.97 to 99.45% to 16S rDNA Methanobrevibacter woesei GS. Sequence CH1270 is 97.62% homologous to the sequence identified to uncultured archaeon clone ConP1-11F. Clearly, the predominant methanogen found to reside in the chicken ceca was M. woesei. By using a MPN enumeration method, methanogen counts were found to be in the range of 6.38 to 8.23 log10 organisms per gram wet weight. The 16S rDNA copy number per gram wet weight in the samples was between log10 5.50 and 7.19. The second phase of the study was conducted to observe the effects of selected nitrocompounds and two different feedstuffs on in vitro methane production in chicken cecal contents and rumen fluid. Initially, one of the three nitrocompounds was added to incubations containing cecal contents from laying hens supplemented with either alfalfa or layer feed. Both feed materials influenced volatile fatty acids (VFA) production and also fostered methane production in the incubations although methane was lower (P < 0.05) in incubations with added nitrocompound, particularly nitroethane. Secondly, nitroethane was examined in incubations of bovine or ovine rumen fluid or cecal contents containing either alfalfa or layer feed. Unlike cecal contents, layer feed significantly (P < 0.05) supported in vitro methane production in incubations of both rumen fluids. The results show that nitroethane impedes methane production, especially in incubations of chicken cecal contents. The final phase of this study was carried out to determine the methanogenic establishment in the chicken ceca by the cultural method with the quantitative PCR. The results suggested that methanogens colonized in chicken ceca at a few days after birth. Litter and house flies could be potential sources for methanogenic colonization in broiler chicks.

In the first phase of this study, the diversity of methanogenic bacteria in avian ceca was found to be minimal. Based on 16S rDNA clone libraries, a common phylotype, designated CH101, ranged between 92.86 to 100 % of the total clones whereas less than 1% of the other phylotypes were found. On the basis of the sequence identity, all of the sequences, except sequence CH1270, are related from 98.97 to 99.45% to 16S rDNA Methanobrevibacter woesei GS. Sequence CH1270 is 97.62% homologous to the sequence identified to uncultured archaeon clone ConP1-11F. Clearly, the predominant methanogen found to reside in the chicken ceca was M. woesei. By using a MPN enumeration method, methanogen counts were found to be in the range of 6.38 to 8.23 log10 organisms per gram wet weight. The 16S rDNA copy number per gram wet weight in the samples was between log10 5.50 and 7.19. The second phase of the study was conducted to observe the effects of selected nitrocompounds and two different feedstuffs on in vitro methane production in chicken cecal contents and rumen fluid. Initially, one of the three nitrocompounds was added to incubations containing cecal contents from laying hens supplemented with either alfalfa or layer feed. Both feed materials influenced volatile fatty acids (VFA) production and also fostered methane production in the incubations although methane was lower (P < 0.05) in incubations with added nitrocompound, particularly nitroethane. Secondly, nitroethane was examined in incubations of bovine or ovine rumen fluid or cecal contents containing either alfalfa or layer feed. Unlike cecal contents, layer feed significantly (P < 0.05) supported in vitro methane production in incubations of both rumen fluids. The results show that nitroethane impedes methane production, especially in incubations of chicken cecal contents. The final phase of this study was carried out to determine the methanogenic establishment in the chicken ceca by the cultural method with the quantitative PCR. The results suggested that methanogens colonized in chicken ceca at a few days after birth. Litter and house flies could be potential sources for methanogenic colonization in broiler chicks.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A presente dissertação é fruto de uma pesquisa sobre a história, teologia e prática de um organismo ecumênico, o Centro Ecumênico de Evangelização, Capacitação e Assessoria CECA. O CECA nasceu com entidade católico-romana e foi evoluindo de uma forma que se tornou ecumênica por causa de diversos fatores, especialmente eclesiásticos. Neste trabalho analisaremos a contribuição do CECA na difusão da Teologia da Libertação no Brasil e suas práticas a partir desse referencial teológico e as implicações da história da entidade com o próprio desenvolvimento da TL na América Latina. Concomitantemente foi se construindo uma proposta de ecumenismo no CECA que foi um aporte significativo para o movimento ecumênico no Brasil. Na memória dos trinta anos de história do CECA se resgatam personagens, práticas e uma proposta de evangelização comprometida com as transformações estruturais da sociedade, como mediações históricas do reino de Deus que têm na unidade dos cristãos como principal princípio para uma nova oikoumene segundo os desígnios de Deus, anunciados pelos profetas bíblicos e encarnados em Jesus Cristo. / The present dissertation is a research about History, Theology and Practice of an ecumenical organism The Ecumenical Center of Evangelization, Formation and Consultancy CECA. It was created as a Roman-Catholic entity and has been growing to become ecumenical because of many, namely ecclesiastic reasons. In this essay, I shall analyze the contribution of CECA toward the proliferation of Liberation Theology in Brazil and its practices based on this theological reference, as well as the interrelatedness between the history of this organization with the development of Liberation Theology in Latin America. Simultaneously, a new ecumenical proposal was being elaborated by CECA which has given significant support to the Ecumenical Movement in Brazil. From the thirty years of its history, many things are to been remembered: persons, practices and a proposal of evangelization engaged with structural transformations of society, as historic mediations of the kingdom of God with Christian unity as its main principle toward a new oikoumene, according to Gods purpose, announced by biblical prophets and embodied in Jesus Christ.

Le microbiote intestinal constitue un élément important pour l’hôte, il est impliqué notamment au niveau immunologique ou physiologique. La connaissance de la composition de ce microbiote est la première étape dans la compréhension des phénomènes qui lui sont associés. Les travaux de cette thèse se sont articulés selon plusieurs objectifs. La première étape a été de faire un état des lieux de la composition du microbiote de deux types génétiques parentaux : le canard Pékin (Anas Platyrhynchos), le canard de Barbarie (Cairina Moschata) ainsi que de leur hybride ; le canard mulard. Nous avons ainsi pu observer des compositions différentes selon le type génétique avec tout de même la prédominance dans chacune des trois espèces de deux phyla : les Firmicutes et les Bacteroidetes. Différentes conditions rencontrées dans l’environnement sont à même de créer un déséquilibre dans la composition du microbiote. Une des conditions possibles est un changement dans l’alimentation ainsi la seconde étape a été de voir l’impact du gavage sur les communautés bactériennes composant le microbiote. Celui-ci induit bien des modifications au sein du microbiote en privilégiant certaines classes bactériennes notamment les Bacilli et les Clostridia selon le type génétique. Un effet du gavage a été montré au niveau du microbiote iléal tandis que l’on retrouve un effet du type génétique ainsi que du gavage mais plus faible que dans l’iléon au niveau des caeca. Deux autres travaux ont été réalisés, le premier concernait l’ajout d’un probiotique (Lactobacillus sakei) sur le microbiote des canards mulards durant la phase de gavage. Nous avons montré que lors de cet ajout, nous observions une forte augmentation des lactobacilli au niveau de l’iléon. Enfin suite aux fortes contaminations retrouvées dans le sud-ouest ainsi que ses effets toxiques montrés dans différentes études, le dernier travail effectué a été de voir l’effet du cadmium sur le microbiote des canards Pékin et Barbarie. Nous avons pu ainsi observer les modifications de la communauté microbienne lors de l’ajout de cadmium ainsi l’accumulation de celui-ci dans les reins au cours du gavage. Des effets combinés entre le cadmium et la période de gavage ainsi qu’avec le type génétique ont été mis en lumière. Une tendance à une accumulation différentielle du cadmium a été observé selon le type génétique. / The intestinal microbiota is an important element for the host; it is particularly involved in immunological or physiological level. Knowledge of the composition of the microbiota is the first step in understanding the phenomena associated with it. The work of this PhD was organized according to several objectives. The first step was to realize a molecular inventory of the microbiota composition of the two parental genetic types: the Pekin duck (Anas Platyrhynchos), the Muscovy duck (Cairina moschata) and their hybrid, the mule duck. We have observed different compositions depending on the genetic type although with a predominance of two phyla: the Firmicutes and Bacteroidetes for all ducks species. Different conditions encountered in the environment are likely to create an imbalance in the composition of the microbiota. One is a change in diet; therefore the second step was to see the impact of overfeeding on bacterial communities. Overfeeding causes many changes in the bacterial microbiota and increase two classes: Bacilli and Clostridia according to the genetic type. The effect of overfeeding has been shown principally on the ileal microbiota while genetics and overfeeding both affected weekly cecal microbiota. We then studied the impact of adding a probiotic strain (Lactobacillus sakei) on the microbiota of mule ducks during the overfeeding period. We could see here a significant effect of this addition only in the ileum with a sharp increase in lactobacilli. Finally, due to high levels of contamination found in the southwest of France and its toxic effects on metabolism shown in various studies, the last work was to see the effect of cadmium on the microbiota of Pekin and Muscovy ducks. We observed changes in the microbial community when adding cadmium and see the accumulation of it in the kidneys during overfeeding. Combined effects between cadmium and the feeding period as well as the genetic type were highlighted. Finally

Thesis--Rijksuniversiteit te Leiden, 1957. / Includes bibliographical references (p. [194]-199) and index.

Thesis--Rijksuniversiteit te Leiden, 1957. / Includes bibliographical references (p. [194]-199) and index.

grainy head (grh) genes encode a family of transcription factors conserved from fly to human. Drosophila grh is the founding member of this gene family and has multiple functions, including tracheal tube size control, epidermal barrier formation and reconstruction after wounding. To understand the underlying molecular mechanism of grh functions, we tried to isolate its direct targets and analyze their function. We identified ten grh targets by combining bioinformatics and genetics. Grh directly controls the expression of stitcher (stit), which encodes a Ret family receptor tyrosine kinase (RTK), during both development and wound healing. Stit promotes actin cable assembly and induces extracellular signal-regulated kinase (ERK) phosphorylation around the wound edges upon injury. Stit also activates barrier repair genes and its own expression at the wound sites in a Grh-dependent manner. This positive feedback loop ensures efficient epidermal wound repair. In addition, Grh regulates the expression of multiple genes involved in chitin biosynthesis or modification. Most of the genes are required for tracheal tube size control. Two of them, verm and serp, encode related putative luminal chitin deacetylases. The functional analysis of verm and serp identifies an important role of luminal chitin matrix modification in limiting tracheal tube elongation. Therefore, it is very likely that Grh controls tracheal tube size through regulating multiple targets involved in the assembly or modification of luminal chitin matrix. Grh also directly activates the epidermal expression of Peptidoglycan recognition protein LC (PGRP-LC) gene that is required for the induction of antimicrobial peptides (AMPs) upon infection. Furthermore, ectopically expressing Grh is sufficient to induce AMP Cecropin A lacZ reporter (CecA-LacZ) in the embryonic epidermis. These results suggest a new function of Grh in the local immune responses in Drosophila barrier epithelia. / At the time of the doctoral defense, the following papers was unpublished and had a status as follows: Paper 1: Manuscript.

Fondée en 1685, la Compagnie de Saint-Gobain est aujourd’hui une très grande firme mondiale. Elle doit à son développement propre, mais aussi à sa fusion avec Pont-à-Mousson. Elle était déjà la première multinationale française au début du XXème siècle. Elle a cherché très tôt l’internationalisation, surtout en direction de l’Europe, s’implantant en Allemagne comme en Italie. Après la deuxième Guerre mondiale, Pont-à-Mousson a pris part à la création de la Communauté économique du charbon et de l’acier (CECA). Cette dernière est considérée comme la première institution conduisant à l’unité européenne, notamment à une Europe économique. Pour les deux entreprises définies chacune comme un centre autonome de décision, il s’agit, dans ce livre, d’étudier le processus de la prise de décision économique, à l’intérieur, d’analyser l’environnement extérieur pendant la période de 1945-1970 dans le contexte de la construction européenne. Pour quelle raison Pont-à-Mousson a-t-il participé avec un tel intérêt à la création de la CECA ? Quel rôle les groupes Saint-Gobain et Pont-à-Mousson ont-ils joué dans l’ouverture du marché commun ? Quels ont été la politique et les objectifs des dirigeants politiques ? L’un des intérêts de l’ouvrage consiste à tenter de définir l’évolution des stratégies passées d’une attitude défensive à une attitude offensive, les deux entreprises à s’adapter à l’Europe et acceptant ainsi la transformation progressive de leur modèle de gestion. C’est très largement la contrainte européenne qu’a poussé à leur rapprochement, sans que celui-ci puisse s’expliquer par la seule pression d’un état français interventionniste : les stratégies d’entreprises elle-même ont joué un rôle déterminant. / Founded in 1685, Company of Saint-Gobain is now a very large global firm, which is due to its own development, but also to its merger with Pont-à-Mousson. In the early twentieth century, the Companies were already the leading French multinational. It sought early internationalization, especially to Europe, establishing itself in Germany and Italy. After World War II, Pont-à-Mousson took part in the creation of the European Coal and Steel Community (ECSC). The latter is considered as the first institution leading to European unity, especially in an economic Europe. For both companies, each defined as an autonomous centre of decision, it is this book that aims to study the inside process of economic decision-making and to analyse the external environment during the 1945-1970 in the context of European integration. Why Pont-à-Mousson has involved with such an interest in the creation of the ECSC? Which role did the groups of Saint-Gobain and Pont-à-Mousson play in the opening of the common market? What were the policy and objectives of the political leaders? One of the interests of this book is to try to define the evolution of strategies, from a defensive attitude to an offensive attitude, the two companies adapt to Europe and thus accepting the gradual transformation in their Management model. This is mostly the European constraint that has pushed them closer, rather than under the only pressure pushed by an interventionist French state: strategies of the Company itself played a decisive role.

Cette thèse s'inscrit dans la nouvelle histoire du droit européen en proposant les résultats de recherches biographiques sur les premiers juges, avocats généraux et greffier qui ont fait fonctionner la Cour de Justice de l'Union européenne pendant les vingt premières années de son existence (1952 - 1972). Il s'agit tout d'abord de mettre en lumière les parcours professionnels et personnels des pionniers de la juridiction et des hommes qui ont prononcé certains de ses jugements les plus connus, ceux qui ont lancé le processus de « constitutionnalisation » de l'ordre juridique communautaire. Ensuite, il s'agit d'utiliser les résultats de ces recherches biographiques pour apporter, sur trois points précis, des éléments de réponse à une question qui intéresse depuis deux décennies en particulier les politistes - et de manière plus récente, les historiens: comment cette institution réussit-elle, à partir des années 1960, à autant limiter la souveraineté des Etats membres, en posant notamment le principe de primauté du droit européen, et cela sans semble-t-il être confrontée à de sérieuses résistances de la part des dirigeants des Etats membres ? Cette thèse analyse tout d'abord le processus de nomination des juges et des avocats généraux, afin de savoir si les gouvernements tentent, ou non, via le mécanisme de sélection de mettre un terme à sa jurisprudence audacieuse. Puis, elle étudie les convictions européennes des membres de la Cour et leur vision du rôle qu'ils doivent jouer dans le processus d'intégration. Enfin, elle s'intéresse au réseau politique des hommes de la juridiction européenne et leurs interactions officieuses avec les dirigeants des Etats membres. / This Ph.D. thesis contributes to the new history of European law by proposing the results of in-depth biographical research on the first judges, advocates general and registrar who worked at the European Court of Justice during the first twenty years of its existence (1952 - 1972). Firstly, it sheds new light on the professional and personal trajectories of the Court's pioneers and of those who pronounced some of its most commented judgments, those who laid the foundation of the 'constitutionalization' of the European legal order. Secondly, it leans on the collected biographical data in order to bring to the surface new elements of knowledge on three precise points with regard to a question which political scientists have been interested in for two decades - and which now also historians address: how did the European Court of Justice, from the 1960s onwards, succeed in seriously limiting the Member States' sovereignty, especially by imposing the principle of primacy of European law, without provoking a rebellion by national decision-makers? It first of all analyzes the nomination process of the first judges and advocates general, in order to establish whether governments did, or did not, use the appointment mechanism to curb down ECJ activism. It then studies the members of the Court's views and convictions with regard to European integration and their role in the European Communities. Finally, it looks at the political affiliations of the first members of the Court of Justice and their unofficial interactions with national decision-makers.

Immédiatement après la Seconde Guerre mondiale, sous l’impulsion du Général de Gaulle et de Jean Monnet, le Gouvernement provisoire de la République française décida d’engager dans le système capitaliste français une politique économique à moyen terme désignée par le terme de « planification ». Le Plan, en tant qu’outil d’élaboration et de mise en œuvre de politiques industrielles, dirigea l’industrie française pour qu’elle puisse se développer et s’adapter aux différentes circonstances. Quatre plans furent successivement mis en œuvre dès la Libération jusqu’en 1965 par le Commissariat Général du Plan (CGP) sous la direction successive de trois commissaires généraux du Plan, à savoir Jean Monnet, Etienne Hirsch, Pierre Massé. Dans ce laps de temps furent lancés les plans suivants : Plan Pinay-Rueff, Plan intérimaire, Plan d’adaptation des charbonnages, Plan de stabilisation. Imbriquée de multiples manières à une « expansion industrielle », la planification française réussit à transformer la France rurale en une société industrielle et à faire passer l’industrie française de la situation fermée et protégée à la confrontation de la concurrence internationale. Elle joua aussi un rôle important dans le mode de rapport entre le secteur public et privé et en particulier, le fonctionnement des entreprises nationales. À plus long terme, c’était encore au Plan que revenait le mérite de tracer les lignes d’un harmonieux développement des régions françaises, surtout de l’aménagement du territoire. / Immediately after the Second World War, the Provisional Government of the French Republic, under the leadership of General de Gaulle and Jean Monnet, decided to engage in the French capitalist system a medium-term economic policy designated by the term "planning". The Plan, as a tool for the elaboration and implementation of industrial policies, directed French industry so that it could develop and adapt to different circumstances. Four Plans were successively implemented from the Liberation until 1965 by the General Planning Agency under the successive direction of three commissioners, namely Jean Monnet, Etienne Hirsch, Pierre Massé. Meanwhile, the Rueff-Pinay Stabilization Plan, Interim Plan, Plan d’adaptation des charbonnages, Giscard d’Estaing’s Stabilization Plan were launched. Interwoven in many ways with an "industrial expansion", French planning succeeded in transforming rural France into an industrial society and to shift French industry from the closed and protected situation to the confrontation of international competition. It also played an important role in the relationship between the public and private sectors and, in particular, the functioning of national enterprises. In the longer term, it was again in the Plan that the merit of drawing the lines of a harmonious development of the French regions, especially of the Spatial planning.