Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The coconut palm (Cocos nucifera L.) is the most palmae cultivated throughout the world. The stem bleeding, caused by the fungus Ceratocystis paradoxa (De Seynes) Moreau, has caused significant losses in production in several states of Brazil, including Sergipe. Etiology and transmission mechanisms are largely unknown and epidemiological studies have not yet been realized. This study aimed to analyze the spatial arrangement of disease in the field, in order to characterize the spread of stem bleeding, besides the use of the technique for analyzing the structure of foci for characterization. The spatial pattern of disease was investigated in three planting areas, each containing 1500 plants / area, divided into quadrats of 15 plants, using the mapping analysis, ordinary runs, a slight adjustment to the distribution of frequency and spatial autocorrrelation. In all areas it was evident the presence of aggregation of diseased plants, indicating a predominant pattern of spatial arrangement of disease in the field. In analyzing the structure of foci, it was found a total of 13 foci, with an average of 27.5 plants / focus and tendency (higher number of symptomatic plants) to grow in the direction of the lines of plantation, with provision so elliptical. From the data analysis, there were indications that possibly the stem bleeding route of transmission has the natural dispersal of inoculum and cultural practices. Are required to carry out additional studies on the dispersal of inoculum for a better understanding of disease dynamics and the establishment of appropriate control measures. / O coqueiro (Cocos nucifera L.) é a palmácea mais cultivada em todo o mundo. A resinose do estipe, causada pelo fungo Ceratocystis paradoxa (De Seynes) Moreau, tem causado perdas significativas na produção em diversos estados do Brasil, entre eles Sergipe. Sua etiologia e mecanismos de transmissão são pouco conhecidos e, estudos epidemiológicos, ainda não foram realizados. Objetivou-se analisar o arranjo espacial da doença no campo, com o intuito de caracterizar a disseminação da resinose, além do emprego da técnica de análise da estrutura de focos para caracterização da doença. O arranjo espacial da doença foi investigado em 3 parcelas de plantio, cada um contendo 1.500 plantas/área utilizou-se análises de mapeamento e ordinary runs , posteriormente as parcelas foram subdivididas em quadriláteros de 15 plantas para o cálculo de ajuste à distribuição discreta de freqüência e autocorrrelação espacial. Em todos os setores ficou evidenciada a presença de agregação de plantas doentes, indicando haver um padrão predominante de arranjo espacial agregado da doença no campo. Na análise da estrutura de focos, constatou-se um total de 13 focos, com média de 27,5 plantas/focos e com tendência (maior número de plantas sintomáticas) de crescerem na direção das linhas de plantios, tendo disposição de forma elíptica. A partir da análise dos dados, houve indicações que possivelmente a resinose tem como via de transmissão a dispersão natural do inóculo e práticas culturais. São necessários a realização de estudos adicionais sobre a dispersão do inóculo para o melhor entendimento da dinâmica da doença e o estabelecimento de medidas de controle adequadas.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / One of the promising ways for the residues utilization is through the development of biotechnological processes for the production of a large number of metabolites of industrial interest, such as the production of bioaromas. The fungus Ceratocystis fimbriata has the potential to synthesis of esters producing varieties of flavor compounds such as ethyl acetate, responsible for a diversity of fruit flavors. The objective this work was utilize and evaluate the biotechnological potential of cassava rind, one of the agro-industrial waste more produced in the Sergipe state, for the production of ethyl acetate through the solid state fermentation. All procedure performed obeyed an experimental design of eleven experiments corresponding to an experimental design 22 trials plus 4 axial points and three repetitions at the central point, with the variable sample mass and moisture content. The volatile compound ethyl acetate was quantified by headspace analysis on a gas chromatograph and it was found that the best experiment for the production of ethyl acetate was (91,92 μmol.L-1) with 50% humidity and 14:23 g weight dried for 48 hours fermentation. As of the best result was done other fermentation for separating the aroma using NaCl at a concentration of 5%, 10%, 15%, 20%, 25%, 30% and 35%. It was observed that NaCl concentration of 30% obtained best value (3303,60 μmol.L-1). This result has been done an increased scale to verify the influence of producing the compound ethyl acetate in a larger surface area where the experiments were performed in 1000 ml Erlenmeyer flask (10%, 20%, 30 % of the quantity of inoculum) and 2000 ml Erlenmeyer flask (50% of the quantity of inoculum). Chromatographic analysis found that 30% of saline best recovered the ethyl acetate in a 1000 ml Erlenmeyer flask with 30% inoculum (19,38 μmol.L-1). / Uma das formas promissoras para o aproveitamento de resíduos é através do desenvolvimento de processos biotecnológicos para produção de um grande número de metabólitos de interesse industrial, como por exemplo, a produção de bioaromas. O fungo Ceratocystis fimbriata tem potencial para síntese de ésteres produzindo variedades de compostos de aromas, como o acetato de etila, responsável por umas diversidades de aromas de frutas. O objetivo deste trabalho foi aproveitar e avaliar o potencial biotecnológico da casca de mandioca, um dos resíduos agroindustriais mais produzidos no estado de Sergipe, para produção de acetato de etila através da fermentação em estado sólido. Todo o procedimento realizado obedeceu a um planejamento experimental de onze experimentos que corresponde um planejamento experimental 22 ensaios acrescidos de 4 pontos axiais e 3 repetições no ponto central, tendo como variáveis a massa da amostra e o teor de umidade. O composto volátil acetato de etila foi quantificado através da análise de headspace no cromatógrafo a gás e foi detectado que o melhor experimento para produção do acetato de etila foi (91,92 μmol.L-1) com 50% de umidade e 14,23 g de massa seca durante 48 horas de fermentação. A partir do melhor resultado obtido foi realizado outra fermentação para separação do aroma utilizando NaCl numa concentração de 5%, 10%, 15%, 20%, 25%, 30% e 35%. Foi observado que a concentração de NaCl de 30% obteve melhor valor (3303,60 μmol.L-1). Com este resultado, foi realizado um aumento de escala para verificar a influência da produção do composto acetato de etila numa maior área superficial onde os experimentos foram realizados em erlenmeyer de 1000 ml (10%, 20%, 30% de quantidade de inóculo) e 2000 ml (50% de quantidade de inóculo). A análise cromatográfica detectou que a solução salina de 30% recuperou melhor o acetato de etila no erlenmeyer de 1000 ml com 30% de inóculo (19,38 μmol. L-1).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 57-64).

Made available in DSpace on 2014-06-11T19:28:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-07-27Bitstream added on 2014-06-13T18:35:00Z : No. of bitstreams: 1 santos_ms_me_botfca.pdf: 562680 bytes, checksum: 6a69838524a6f17b5f28de9bffb58525 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A intensificação da atividade antrópica tem resultado em significativo aumento da concentração de CO2 atmosférico, o qual poderá provocar alterações no ciclo das doenças de plantas. Assim, foram realizados dois ensaios em ambiente controlado com o objetivo de avaliar o efeito de elevadas concentrações de CO2 sobre o desenvolvimento da murcha causada por Ceratocystis fimbriata em dois clones de eucalipto (um híbrido de Eucalyptus urophylla x E. camaldulensis e outro da espécie E. urophylla). Mudas clonais de eucalipto foram cultivadas em tubetes mantidos em caixas plásticas com injeção de ar e CO2 puro até atingir as concentrações de 550 e 900 μmol mol-1 e em caixas com e sem a injeção de ar (sem injeção de CO2), que foram utilizadas como testemunhas (380 e 390 μmol mol-1, respectivamente). Após 70 dias de crescimento nesses ambientes no primeiro ensaio e 57 no segundo, as hastes das mudas foram inoculadas com 100μL de uma suspensão de conídios de C. fimbriata (2,5x105 mL-1). Nos ambientes com elevada concentração de CO2, as mudas apresentaram menor severidade e incidência da doença, assim como um maior período de incubação. O desenvolvimento das mudas de eucalipto (altura, diâmetro da haste, área foliar, peso de raízes e parte aérea) foi maior nos tratamentos com injeção de CO2, diferindo significativamente das testemunhas. No primeiro ensaio, a análise do teor de carbono das folhas e hastes das mudas de eucalipto não apresentou diferenças entre os ambientes com e sem injeção de CO2 / The disturbances caused by the intensification of human activity have resulted in a significant increase in atmospheric CO2 concentration, which may cause changes in the cycle of plant diseases. Thus, two trials were performed in a controlled environment in order to evaluate the effect of elevated CO2 concentrations was evaluated on the development of wilt caused by Ceratocystis fimbriata in two clones of eucalyptus (hybrid of Eucalyptus urophylla x E. camaldulensis and another hybrid of E. urophylla). The seedlings were grown in tubettes kept in plastic boxes with injection of air and pure CO2 until it reaches the concentrations of 550 and 900 μmol mol-1 and in boxes with and without the injection of air (without CO2 injection), which were used as controls (380 and 390 μmol mol-1, respectively). After 70 days of growth in these environments in the first trial and 57 in the second the stems of the plantlets were inoculated with 100 mL of a conidial suspension of C. fimbriata (2.5 x 105 mL-1). In settings with high concentrations of CO2, the plantlets had lower severity and incidence of the disease, as well as longer incubation period. The development of eucalyptus plantlets (height, stem diameter, leaf area, weight of roots and shoots) was higher in treatments with CO2 injection, differing significantly from controls. In the first trial, carbon analysis from leaves and stems of plantlets of eucalyptus showed no differences between settings with and without CO2 injection. The carbon content of roots was significantly lower in plantlets held at 550 μmol mol-1 CO2 compared to those maintained at 390 μmol mol-1 CO2. In the second trial, from leaves and stems of plantlets in high CO2 concentrations had higher C content compared to the control 380 μmol mol-1 CO2

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T11:29:59Z No. of bitstreams: 2 ariadne_marques.pdf: 1829125 bytes, checksum: 01fca719846b682abc79cca9f78f836a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T11:30:33Z (GMT) No. of bitstreams: 2 ariadne_marques.pdf: 1829125 bytes, checksum: 01fca719846b682abc79cca9f78f836a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T11:31:10Z (GMT) No. of bitstreams: 2 ariadne_marques.pdf: 1829125 bytes, checksum: 01fca719846b682abc79cca9f78f836a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-01-06T11:31:10Z (GMT). No. of bitstreams: 2 ariadne_marques.pdf: 1829125 bytes, checksum: 01fca719846b682abc79cca9f78f836a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O Ceratocystis fimbriata Ellis & Halsted ? um fitopat?geno vascular de ampla distribui??o geogr?fica e gama de hospedeiros. A murcha de Ceratocystis em Eucalyptus ? considerada de dif?cil controle devido ao seu car?ter sist?mico e a variabilidade gen?tica dos isolados. O plantio de material resistente ? a principal forma de controle desta doen?a. No entanto, a obten??o de material gen?tico resistente pode demandar longos per?odos em um programa de melhoramento. A identifica??o de gen?tipos resistentes dentre os clones utilizados comercialmente pode ajudar a abreviar o tempo necess?rio ao melhoramento. Assim, objetivou-se avaliar a resist?ncia de clones de eucalipto ? murcha de Ceratocystis, analisar as respostas ecofisiol?gicas desses clones sob infec??o e fornecer suporte ao processo de melhoramento gen?tico para tal caracter?stica. Foram avaliados 27 clones de resist?ncia desconhecida e 2 clones caracterizados como resistente e suscet?vel ? murcha de Ceratocystis, todos inoculados artificialmente com isolados de C. fimbriata. Os 27 clones apresentaram, aos 50 dias ap?s inocula??o, varia??o quanto ? resist?ncia ? doen?a. Os dois clones de resist?ncia conhecida tiveram crescimento, ?ndice de clorofila e efici?ncia qu?ntica do fotossistema II (Fv/Fm) avaliados em quatro diferentes tempos. As vari?veis analisadas apresentaram potencial para uso na avalia??o da resist?ncia/suscetibilidade de gen?tipos de Eucalyptus spp. ? murcha de Ceratocystis. O clone resistente (R) e o suscet?vel (S) foram utilizados para a constru??o de uma biblioteca subtrativa supressiva. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT The Ceratocystis fimbriata Ellis & Halsted is a vascular phytopathogen of wide geographic distribution and host range. Ceratocystis wilt in Eucalyptus is considered difficult to control due to its systemic character and the genetic variability of the isolated. Planting resistant material is the main way to control this disease. However, the acquisition of genetic material resistant may require long periods in a breeding program. The identification of resistant genotypes among clones used commercially can help shorten the time needed to improve. The aim was to evaluate the resistance of eucalyptus clones to Ceratocystis wilt, analyze the ecophysiological responses of these clones under infection and provide support to the process of breeding for this trait. We evaluated 27 clones of unknown resistance and 2 clones characterized as resistant and susceptible to Ceratocystis wilt, all artificially inoculated with isolates of C. fimbriata. The 27 clones showed, 50 days after inoculation, variation in disease resistance. The two clones of known resistance grew, chlorophyll content and quantum efficiency of photosystem II (Fv / Fm) evaluated at four different times. The variables analyzed showed potential for use in the evaluation of the resistance/susceptibility of genotypes of Eucalyptus spp. to Ceratocystis wilt. The clone resistant (R) and susceptible (S) were used for the construction of a suppressive subtractive library.

Orientador: Raquel Ghini / Banca: Edson Luiz Furtado / Banca: Celso Garcia Auer / Resumo: A intensificação da atividade antrópica tem resultado em significativo aumento da concentração de CO2 atmosférico, o qual poderá provocar alterações no ciclo das doenças de plantas. Assim, foram realizados dois ensaios em ambiente controlado com o objetivo de avaliar o efeito de elevadas concentrações de CO2 sobre o desenvolvimento da murcha causada por Ceratocystis fimbriata em dois clones de eucalipto (um híbrido de Eucalyptus urophylla x E. camaldulensis e outro da espécie E. urophylla). Mudas clonais de eucalipto foram cultivadas em tubetes mantidos em caixas plásticas com injeção de ar e CO2 puro até atingir as concentrações de 550 e 900 μmol mol-1 e em caixas com e sem a injeção de ar (sem injeção de CO2), que foram utilizadas como testemunhas (380 e 390 μmol mol-1, respectivamente). Após 70 dias de crescimento nesses ambientes no primeiro ensaio e 57 no segundo, as hastes das mudas foram inoculadas com 100μL de uma suspensão de conídios de C. fimbriata (2,5x105 mL-1). Nos ambientes com elevada concentração de CO2, as mudas apresentaram menor severidade e incidência da doença, assim como um maior período de incubação. O desenvolvimento das mudas de eucalipto (altura, diâmetro da haste, área foliar, peso de raízes e parte aérea) foi maior nos tratamentos com injeção de CO2, diferindo significativamente das testemunhas. No primeiro ensaio, a análise do teor de carbono das folhas e hastes das mudas de eucalipto não apresentou diferenças entre os ambientes com e sem injeção de CO2 / Abstract: The disturbances caused by the intensification of human activity have resulted in a significant increase in atmospheric CO2 concentration, which may cause changes in the cycle of plant diseases. Thus, two trials were performed in a controlled environment in order to evaluate the effect of elevated CO2 concentrations was evaluated on the development of wilt caused by Ceratocystis fimbriata in two clones of eucalyptus (hybrid of Eucalyptus urophylla x E. camaldulensis and another hybrid of E. urophylla). The seedlings were grown in tubettes kept in plastic boxes with injection of air and pure CO2 until it reaches the concentrations of 550 and 900 μmol mol-1 and in boxes with and without the injection of air (without CO2 injection), which were used as controls (380 and 390 μmol mol-1, respectively). After 70 days of growth in these environments in the first trial and 57 in the second the stems of the plantlets were inoculated with 100 mL of a conidial suspension of C. fimbriata (2.5 x 105 mL-1). In settings with high concentrations of CO2, the plantlets had lower severity and incidence of the disease, as well as longer incubation period. The development of eucalyptus plantlets (height, stem diameter, leaf area, weight of roots and shoots) was higher in treatments with CO2 injection, differing significantly from controls. In the first trial, carbon analysis from leaves and stems of plantlets of eucalyptus showed no differences between settings with and without CO2 injection. The carbon content of roots was significantly lower in plantlets held at 550 μmol mol-1 CO2 compared to those maintained at 390 μmol mol-1 CO2. In the second trial, from leaves and stems of plantlets in high CO2 concentrations had higher C content compared to the control 380 μmol mol-1 CO2 / Mestre

Ceratocystis fimbriata is the type species for the genus Ceratocystis and was first described as the causal agent of black rot in sweet potatoes. However, evidence from DNA sequence data suggests that C. fimbriata is in fact a species complex (C. fimbriata sensu lato) consisting of many morphologically similar cryptic species. Species in this complex are pathogens of important root and fruit crops and trees in the forestry industry world-wide. Population studies on some of these species have mainly relied on microsatellite markers. However, nothing is known regarding the microsatellite structure within Ceratocystis species or any species in the order Microascales in which Ceratocystis resides. The need for a more robust identification tool is also required to differentiate between species in this complex. The first chapter of this thesis provides a review of the literature on microsatellite markers, particularly in fungi. It also discusses the history of microsatellites, mechanisms of microsatellite evolution and functional importance in selected fungal examples. In addition, isolation methodologies are compared and contrasted to newly developed techniques that include bioinformatic searches of genome sequences. Opportunities to use and develop microsatellite markers in Ceratocystis species is also discussed with an emphasis on the possibilities that more microsatellites markers would provide. Microsatellites are abundant in eukaryotic genomes, and fungi are no exception. Analyses of microsatellite content in eukaryotic and fungal genomes have shown that fungi contain fewer microsatellites and that each organism shows preference for particular motifs. In Chapter 2 of this thesis, the abundance and distribution of microsatellites in the recently sequenced C. fimbriata genome is investigated. Comparisons to other fungi and eukaryotes show that C. fimbriata follows the general pattern of microsatellite structure, however it is unique in its preference for certain motifs. The C. fimbriata sensu lato species complex contains morphologically indistinct species. Microsatellite markers previously developed for a population study could differentiate between some of the cryptic species based on their geographic location and host-specificity. In Chapter 3 a subset of microsatellite markers identified in gene regions in Chapter 2 are used to develop a diagnostic test to differentiate between species in the complex. Microsatellite markers that are polymorphic between species but monomorphic within species were selected for this purpose. However, not all species could be distinguished using this diagnostic test. This thesis is presented as a series of chapters in which Chapters 3 2 and 3 are in manuscript format. Consequently each chapter represents an independent article and repetition between these chapters has been unavoidable. / Dissertation (MSc)--University of Pretoria, 2014. / Genetics / Unrestricted

This thesis concerns the study of selected Ceratocystis species and Ophiostoma species infecting wounds on broad-leaved trees, particularly those occurring in Africa. However, two chapters also deal with these fungi from Australia, Norway, Sweden and Austria. The dissertation is comprised of a literature review, followed by four research chapters, addressing the occurrence of Ceratocystis spp. and Ophiostoma spp. in Africa, Australia and Norway. The first chapter of the dissertation is a review of Ceratocystis spp. and Ophiostoma spp. with particular reference to Africa, hardwood tree species and wound infections. The review highlights the importance of wood and trees, especially on the African continent, and discusses the threat of deforestation. This is despite efforts by many African countries to establish forests of non-native tree species to address the demand for wood and wood products on the continent. Reforestation is associated with increased risks of the introduction of pests and pathogens, including species of Ceratocystis and Ophiostoma. The taxonomic history of these two fungal genera is summarized, and the review further focuses on the economically important species in these genera, particularly those infecting hardwood tree species on the African continent. The lack of information regarding Ceratocystis spp. and Ophiostoma spp. on hardwood trees in Africa is thus highlighted. Furthermore, the review summarised the dispersal mechanisms of these pathogens, highlighting dispersal too and infection of wounds. Ceratocystis pirilliformis was described in 2003 and it is the only species in the Ceratocystis fimbriata species complex that has pear-shaped ascomatal bases. This fungus was first described from Australia where Eucalyptus spp. are endemic. It was later reported from South Africa on Eucalyptus grandis trees. Chapter two of this dissertation attempts to address questions regarding the geographic distribution, impact and origin of C. pirilliformis in South Africa. This was in line with the fact that it has been suggested that the fungus is likely native to Australia. To address this question, surveys were conducted in many Eucalyptus planting areas in South Africa and the genetic diversity of the fungus in the country was investigated using microsatellite markers previously developed for C. fimbriata. C. pirilliformis was found in three Eucalyptus-growing areas of South Africa, which has considerably increased the known geographic range of the fungus in South Africa. The gene diversity as well as the genotypic diversity for the fungus was found to be very low in the country and the population is apparently clonal. Results thus support the view that C. pirilliformis was accidentally introduced into South Africa. In chapter three of this dissertation, O. quercus is reported for the first time from wounds on non-native Acacia mearnsii in Uganda. In addition a new Pesotum sp., P. australi prov. nom. is described from wounds on native A. mearnsii in Australia. This fungus resembles other Pesotum anamorphs of Ophiostoma in many ways, especially species of the O. piceae complex. However, it can be distinguished from these species by many morphological traits and also based on phylogenetic inference. The closest phylogenetic neighbor of P. australi prov. nom. is O. quercus. The fact that it was isolated from A. mearnsii in Australia indicates that it is probably a native fungus in that area. In chapter four, two Ceratocystis spp. and one Ophiostoma sp. are described as new to science, from wounds on native broad-leaved tree in South Africa. Three other Ophiostomaspp. are also reported in this study. Until recently, very little research has been done with regard to Ceratocystis spp. and Ophiostoma spp. occurring on native tree species in Africa. However, results presented in this chapter strongly suggest that these fungi are common on native trees in Africa and many other species, including potential pathogens await discovery. Chapter five of the dissertation reports, for the first time, Ophiostoma catonianum, O. pluriannulatum and O. quercus from native broad-leaved trees in Norway. It also reports O. catonianum for the first time from Austria and O. quercus for the first time from Sweden. In the past, very little research has been undertaken to explore the diversity of these fungi on hardwood trees in the Nordic countries or other parts of Europe, where most research has been focused on Ceratocystis spp. and Ophiostoma spp. associated with conifer-infesting bark beetles. This chapter represents a preliminary study with important discoveries. It indicates that these fungi are common on wounds on hardwood trees in Europe and emphasizes the importance of expanding these studies in the Nordic countries, to include more hosts and geographic areas. Such studies will almost certainly reveal more species and possibly new species of Ceratocystis and Ophiostoma. / Dissertation (MSc)--University of Pretoria, 2010. / Microbiology and Plant Pathology / Unrestricted

No description available.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Pathogens of the genus Ceratocystis are polyphagous, attacking several species of economic importance, such as black wattle, eucalyptus, mango and cocoa, these have wide variability even within a single genus or species, the result of adaptations to the environment they live in and the hosts available. Study the behavior of these pathogens such as Ceratocystis fimbriata in forest species is important to understand the ways of survival variability and fungus resistance mainly native species. The use of biocontrol as of the genera Trichoderma and Bacillus, have been widely studied as an alternative for the biological control of soil borne pathogens. Hence, the present work aims to study the behavior of different isolates of C. fimbriata obtained from kiwi (Actinidia deliciosa) in forest species as black wattle (Acacia mearnsii), guava (Psidium cattleianum), araucaria (Araucaria angustifolia), cherry (Eugenia involucrata) and inga (Inga marginata); potential biocontrol in vitro Trichoderma spp. and Bacillus subtilis about C. fimbriata and the in vivo behavior of B. subtilis in seedlings of black wattle , against C. fimbriata as an alternative control . For this purpose, we used isolates of C. fimbriata obtained and identified in kiwi plantations. In the pathogenicity test isolates were evaluated for their pathogenic variability and behavior on seedlings of black wattle, guava, araucaria, cherry and inga. Through direct confrontation testing was analyzed in vitro antagonistic potential of Trichoderma spp. and B. subtilis on the pathogen. For the evaluation of the in vivo behavior of B. subtilis black wattle seedlings were inoculated with product Rizolyptus®, seven days before and seven days after pathogen inoculation. Seedlings inoculated with native species C. fimbriata showed no sign of wilting or discoloration of the tissues. However, in seedlings of Acacia mearnsii isolates of C. fimbriata exhibited severity percentage ranging from 44.15 to 100%. In direct confrontation testing isolates of Trichoderma spp. and B. subtilis demonstrated to be effective in vitro on biocontrol isolates of C. fimbriata with percentages of inhibition ranging from 46.48 to 57.76 and from 14.12 to 32.20 % respectively. In biocontrol in vivo test, the Rizolyptus® product was not effective in controlling C. fimbriata and all black wattle seedlings inoculated with the pathogen showed symptoms of wilting and death. / Patógenos do gênero Ceratocystis são polífagos, atacando diversas espécies com importância econômica, como a acácia-negra, eucalipto, mangueira e cacau, estes possuem ampla variabilidade, mesmo dentro de um único gênero ou espécie, resultado de adaptações ao meio em que vivem e aos hospedeiros disponíveis. Estudar o comportamento destes patógenos, como Ceratocystis fimbriata, em espécies florestais é importante para compreender as formas de sobrevivência, variabilidade e resistência do fungo principalmente em espécies nativas. O uso de biocontroladores como, dos gêneros Trichoderma e Bacillus, tem sido largamente estudado como alternativa para o controle biológico de patógenos de solo. Diante disto, o presente trabalho tem como objetivo estudar o comportamento de diferentes isolados de C. fimbriata obtidos de kiwi (Actinidia deliciosa) em espécies florestais como, acácia-negra (Acacia mearnsii), araçá (Psidium cattleianum), araucária (Araucaria angustifolia), cerejeira (Eugenia involucrata) e ingá (Inga marginata); o potencial de biocontrole in vitro de Trichoderma spp. e Bacillus subtilis sobre C. fimbriata e o comportamento in vivo de B. subtilis em mudas de acácia-negra, frente a C. fimbriata, como alternativa de controle. Para tanto, foram utilizados isolados de C. fimbriata obtidos e identificados em plantios de kiwi. No teste de patogenicidade cruzada os isolados foram avaliados quanto à sua variabilidade patogênica e o comportamento sobre mudas de acácia-negra, araçá, araucária, cerejeira e ingá. Através de testes de confrontação direta foi analisado o potencial antagônico in vitro de Trichoderma spp. e B. subtilis sobre o patógeno. Para a avaliação do comportamento in vivo de B. subtilis as mudas de acácia-negra foram inoculadas com o produto Rizolyptus®, sete dias antes e após a inoculação do patógeno. As mudas de espécies nativas inoculadas com C. fimbriata não exibiram sintoma de murcha ou descoloração dos tecidos. Entretanto, em mudas de acácia-negra os isolados de C. fimbriata exibiram percentuais de severidade que variam entre 44,15 a 100%. Nos testes de confrontação direta os isolados de Trichoderma spp. e B. subtilis demostraram-se eficientes no biocontrole in vitro sobre os isolados de C. fimbriata com percentuais de inibição variando de 46,48 a 57,76 e 14,12 a 32,20% respectivamente. No teste de biocontrole in vivo, o produto Rizolyptus® não foi eficiente no controle de C. fimbriata e todas as mudas de acácia-negra inoculadas com o patógeno apresentaram sintomas de murcha e morte.