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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Bispecific monoclonal antibodies : their potential advantages in immunoassay systems

Cook, Adrian Gerald January 1992 (has links)
No description available.
2

Oxalate analysis and its applications

Fry, Ian David Robert January 1991 (has links)
No description available.
3

Rare syndromes of perturbed insulin action and production : application of exome sequencing and characterisation of their cellular phenotypes

Raffan, Eleanor January 2013 (has links)
No description available.
4

The role of nucleophosmin fusion sequences in the oncogenic activation of the (2;5) translocation protein, nucleophosmin-anaplastic lymphoma kinase (NPM-ALK)

Bischof, Daniela January 1996 (has links)
A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, South Africa, in fulfillment of the requirements for the degree of Doctor of Philosophy. Johannesburg, 1996 / The NPM-ALK fusion gene, formed by the t(2;5)(p23;q35) in non-Hodgkin's lymphoma encodes a 75kDa hybrid protein that contains the amino-terminal 118 amino acid residues of the nucleolar phosphoprotein nucleophosmin (NPM) joined to the entire cytoptasmic portion of the receptor tyrosine kinase, anaplastic lymphoma kinase (ALK). The transforming ability of NPM-ALK is demonstrated and it is shown that oncogenesis by the chimaeric protein requires the activation of its kinase function as a result of oligomerisation mediated by the NPM segment. [Abbreviated Abstract. Open document to view full version]
5

The role of biomedical knowledge in medical diagnosis by learners

Woods, Nicole Natasha. Brooks, Lee R. January 2005 (has links)
Thesis (Ph.D.)--McMaster University, 2006. / Supervisor: Lee R. Brooks and Geoffrey R. Norman. Includes bibliographical references (leaves 81-90). Mode of access: World Wide Web.
6

Prevalência dos antígenos eritrocitários caninos em cães domésticos (Canis familiaris) e investigação dos parâmetros hematológicos e da ocorrência de antígenos eritrocitários em lobos-guará (Chrysocyon brachyurus) e cachorros-do-mato (Cerdocyon thos) criados no Brasil

Novais, Adriana Alonso [UNESP] 31 January 2003 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:32:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-01-31Bitstream added on 2014-06-13T21:05:04Z : No. of bitstreams: 1 novais_aa_dr_jabo.pdf: 165876 bytes, checksum: f8f9bf00cfe11abd0988d6ea3897a700 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O propósito desse estudo foi verificar a prevalência dos antígenos eritrocitários caninos em cães domésticos criados no Brasil e compará-la com aquela descrita na literatura consultada, para cães oriundos de outros países. Além disso, verificar os valores sangüíneos normais e a ocorrência dos antígenos eritrocitários caninos em lobos-guará e cachorros-do-mato, na expectativa de adicionar novos dados sobre valores sangüíneos de referência e investigar as relações filogenéticas entre os caninos silvestres e domésticos. Para tanto, obteve-se amostras de sangue de 200 cães domésticos, sendo 150 cães mestiços e 50 Pastores Alemães, oriundos do município de Jaboticabal - São Paulo, 32 lobos-guará e 16 cachorros-do-mato, pertencentes aos zoológicos de São Carlos - SP, Rio de Janeiro - RJ, São José do Rio Preto - SP, Brasília - DF, Belo Horizonte - MG, Sorocaba - SP, e também do criadouro conservacionista da Companhia Brasileira de Metalurgia e Mineração (CBMM), localizado em Araxá - MG. A prevalência dos grupos sangüíneos encontrada para os mestiços (SRD) foi: 85 (57%) positivos para o grupo DEA 1.1; 61 (41%) positivos para o grupo DEA 1.2; 19 (13%) positivos para o grupo DEA 3; 140 (93%) positivos para o grupo DEA 4; 11 (7%) positivos para o grupo DEA 5 e 17 (11%) positivos para o grupo DEA 7. As combinações de grupos sangüíneos mais observadas foram DEA 1.1,4 (35%) e DEA 1.2,4 (32,5%). A prevalência encontrada para os Pastores Alemães foi: 32 (64%) positivos para o grupo DEA 1.1; 18 (36%) positivos para o grupo DEA 1.2; 4 (8%) positivos para o grupo DEA 3; 50 (100%) positivos para o grupo DEA 4; 7 (14%) positivos para o grupo DEA 5 e 4 (8%) positivos para o grupo DEA 7 (Figura 1). As combinações de grupos sangüíneos mais observadas foram DEA 1.1,4 (50%) e DEA 1.2,4 (28%)... / The goal of this study was to verify the prevalence of dog erythrocyte antigen (DEA) in domestic dogs reared in Brazil, in order to compare with the described prevalence found in the literature for dogs reared in other contries. Also, to verify normal blood values and the occurrence of dog erythrocyte antigen in maned wolf and crab eating dog, to investigate phylogenetic relationship between wild and domestic dogs. For this purpose, we collected anticoagulated blood samples from 200 domestic dogs (150 mixed breed and 50 German Sheepherd - GSH) from the city of Jaboticabal, in Sao Paulo state, and 32 maned wolves and 16 crab eating dogs, from zoos located in São Carlos-SP, Rio de Janeiro-RJ, São José do Rio Preto-SP, Brasília-DF, Belo Horizonte-MG, Sorocaba-SP and from a conservationist breeder called CBMM, located in Araxá/MG. The obtained prevalence of dog erythrocyte antigens in mongrels was the following: 57% positive for DEA 1.1, 41% positive for DEA 1.2, 13% positive for DEA 3, 93% positive for DEA 4, 7% positive for DEA 5 and 11% positive for DEA 7. The obtained blood group prevalence for GSH dogs was: 64% positive for DEA 1.1, 36% positive for DEA 1.2, 8% positive for DEA 3, 100% positive for DEA 4, 14% positive for DEA 5 and 8% positive for DEA 7. The most common combinations of blood groups encountered were DEA 1.1,4 (representing 50% in mongrels and 35% in GSH) and DEA 1.2,4 (representing 28% in mongrels and 32,5% in GSH. The high prevalence of DEA 1 blood group in mongrel and GSH dogs represents a favorable factor, once it reduces the risk for a transfusion reaction. The risk for a inicial transfusion reaction against groups DEA 3, DEA 5 or DEA 7 is 7,6% for mongrel and 6% for GSH dogs... (Complete abstract, access undermentioned eletronic address)
7

Caracterização das alterações laboratoriais e histopatológicas associadas à obstrução uretral experimentalmente induzida em ratos

Costa, Hugo Leonardo Riani [UNESP] 11 December 2008 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:23:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-12-11Bitstream added on 2014-06-13T19:51:02Z : No. of bitstreams: 1 costa_hlr_me_botfmvz.pdf: 1355195 bytes, checksum: 2992cd80d156a33c682f6ce67ff9724e (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A obstrução uretral é uma emergência clínica freqüente no atendimento de pequenos animais. Com a evolução do quadro, ocorre parada na filtração glomerular e, consequentemente, desenvolvem-se várias alterações nos equilíbrios hídrico, eletrolítico e ácido-básico, além do acúmulo de metabólitos nitrogenados e toxinas orgânicas. Podem ocorrer modificações histopatológicas nos rins e na bexiga. Objetivou-se, neste estudo, caracterizar prospectivamente as alterações laboratoriais e histopatológicas de ratos apresentando obstrução uretral. Para tanto, foram utilizados 21 ratos Wistar com obstrução uretral induzida. Foram realizados os seguintes exames: hemogasometria venosa e determinação dos níveis de uréia, creatinina, sódio, potássio, cloreto, cálcio e fósforo. As avaliações foram repetidas a cada 8 horas durante 24 horas. Após esse período os animais foram eutanasiados e as bexigas e os rins enviados para exame histopatológico. Entre os exames bioquímicos, foram observadas elevações estatisticamente significativas nos níveis de uréia, creatinina, fósforo, magnésio e potássio, e diminuição nos níveis de cloreto. Com relação à hemogasometria, houve diferença estatisticamente significativa entre os valores de pH, PO2, PCO2, excesso de base, saturação de oxigênio e lactato. O exame histopatológico renal revelou a presença de alterações tubulares e glomerulares, enquanto a análise histopatológica das bexigas demonstrou a presença de hemorragia, separação de fibras musculares e infiltrado inflamatório. Conclui-se que a obstrução uretral provoca alterações que podem ser detectadas nos exames laboratoriais, sendo as mesmas agravadas no decorrer do tempo. Além disso, a persistência durante 24 horas é capaz de levar a alterações morfológicas no trato urinário. / Urethral obstruction is a frequent emergency in Veterinary clinics. The persistent urethral obstruction leads to blockage of renal filtration, resulting in several alterations in fluid, electrolyte and acid-base balance, besides the accumulation of nitrogenous metabolic products and organic toxins. Histopathological changes may occur in the kidneys and urinary bladder. Thus, this study aimed to prospectively characterize renal and vesical histopathological alterations in rats due to urethral obstruction. Twenty-one male Wistar rats (Rattus norvegicus) with experimental urethral obstruction were included in the study. Venous gasometry and determination of urea, creatinine, sodium, potassium, chloride, calcium and phosporus were performed. The avaliations were repeted each 8 hours during 24 hours. After that period, the animals were euthanatized for the collection of kidneys and bladder fragments to the histopathological exam. Biochemistry exams demonstrated statiscally significant elevations for the levels of urea, creatinine, phosporus, magnesium and potassium, and a decrease for the levels of chloride. Results of gasometry also demonstrated statiscally significant changes for pH, PO2, PCO2, base excess, oxygen saturation and lactate values. Histopathology analysis revealed kidney alterations in tubular and glomerular elements. The most important alterations found in urinary bladders were transmural hemorrhage, separation of muscle fibers and neutrophilic inflammatory infiltrate. Complete urethral obstruction induces important changes that can be detected by laboratorial exams, and the alterations worsen with the course of time. Besides that, the persistent obstruction during 24 hours is able to cause morphological changes in the kidneys and urinary bladder, which can be detected using histopathological exam.
8

The effects of Tulbaghia violacea (wild garlic) leaf and bulb extracts on an oesophageal cancer cell line (SNO)

Moonsamy, Suri. 23 October 2013 (has links)
Ethnopharmacological relevance: Indigenous plants such as Tulbaghia violacea(TV) and Allium sativum (garlic) are traditionally used as natural remedies to treat a variety of ailments, including cancer. This study investigated the effects of TV leaf and bulb extracts and garlic extract on a cancerous oesophageal cell line (SNO). Materials and methods: The methylthiazoltetrazolium (MTT) assay was used to determine the IC50 of TV leaf (TVL) (250μg/ml) and TV bulb extracts (TVB) (25μg/ml) and garlic (500μg/ml). Extracts were treated individually and in combination for a period of 24 hours. Oxidative damage and intracellular glutathione levels were assessed using the Thiobarbituric Acid Reactive Substances (TBARS) Assay and GSH-Glo™ Luminometry Assay, respectively. The CellTiter-Glo® Luminescent Cell Viability Assay was used to assess ATP activity. Induction of apoptosis and mitochondrial membrane potential were determined via the Caspase-Glo® 3/7 Assay, Caspase-Glo® 8 Assay, Caspase-Glo® 9 Assay and JC-1 Mitoscreen Assay, respectively. Morphological apoptotic changes were determined using the Hoechst 33342 stain. Expressions of p53, PARP and NFKB activities were determined by western blotting. Results: Bulb and leaf extracts of TV increased lipid peroxidation compared to the control (p>0.05), whilst garlic and combination of TV leaf and bulb (TVB + TVL) extracts significantly decreased lipid peroxidation relative to the control (p< 0.05). Endogenous glutathione levels significantly decreased in all TV treatments compared to the control (p<0.05).However, garlic was accompanied by insignificantly increased intracellular glutathione levels compared to the control (p> 0.05). The percentages of depolarised mitochondria in all treated cells were significantly decreased compared to untreated cells (p< 0.05). ATP levels increased significantly in garlic and combination (TVB + TVL) treated cells as compared to the control (p< 0.05), yet no significant differences were noted in TVL and TVB treatments (p> 0.05). Caspase8 and caspase 9 activities significantly increased in garlic and combination treated cells relative to the control (p<0.05). A similar trend was noted for caspase 3/7 activity in garlic and combination treatments (p< 0.05). However, initiator and executioner activities in TVL (p> 0.05) and TVB (p> 0.05) treatments did not significantly differ from the control (p> 0.05). All treatments (including garlic) resulted in increased DNA fragmentation and condensation. All treatments decreased p53 expression (p< 0.05), PARP expression (p< 0.05) and NFK B expression (p>0.05) compared to the control. Conclusions: All TV extracts and garlic induces apoptosis in the oesophageal cancerous SNO cell line through changes in oxidative stress, antioxidant systems, and nuclear chromatin condensation, as well as through induction of nuclear genes and signalling pathways. Since inhibition of apoptosis is a principal alteration in cancer, induction of apoptosis would result in a decrease in cancer cell growth. Thus, TV could be exploited as a potential anti-cancer agent. / Thesis (M.Med.Sc.)-University of KwaZulu-Natal, Durban, 2012.
9

Neutrophil tissue inhibitor of matrix metalloproteinases-1 (TIMP- 1) : novel localisation, mobilisation and possible role.

Price, Brendon. 15 November 2013 (has links)
At the beginning of this study, the granule localisation and regulation of release of human neutrophil (PMNL) precursor collagenases, proMMP-8 and -9 (type I and type TV/V collagenases, respectively), enzymes highly active against the extracellular matrix (ECM) and thought to be relevant in invasion and inflammation, had been established while that of their inhibitor, tissue inhibitor of matrix metalloproteinases-I (TIMP-1), had not. Electron microscopy immunogold labelling of cryoultramicrotomy sections for granule marker proteins, lysosome-associated membrane proteins (LAMPs) and endocytosed bovine serum albumin-coated gold probes, followed by stereology, established that TIMP-1 was mainly located in a distinct oval, electron translucent organelle, a little larger than azurophil granules. A lack of labelling for endocytic markers and for glycosylphosphatidylinositol-anchored proteins, established using granule fractionation and immunolabelling to be markers for the secretory vesicles, and LAMPs-1 and -2, indicated the non-endosomal, non-secretory and nonlysosomal nature of this organelle. Density gradient cofractionation with the least dense secretory vesicle population and some pleiomorphism of the organelle suggested that it is a "vesicle" rather than a "granule" population. Colocalisation with proMMP-9 in minor subpopulations suggests that TIMP-1 vesicle biogenesis occurs between metamyelocytic and termination differentiation, but before secretory vesicle synthesis. Immunolabelling of phagocytosed and pulse-chased IgG-opsonised latex beads showed that specific and azurophil granules and a small number of proMMP-8-containing granules (a specific granule subpopulation) fuse with the phagosome whereas the TIMP-1 vesicle and proMMP-9-containing granules do not, suggesting that the latter play no role in phagosomal destruction of IgG-opsonised bacteria and that their phagosomal release is not calcium regulated. However, studies using the calcium ionophore, ionomycin, and monitoring extracellular granule marker protein release upon addition of increasing levels of extracellular calcium, showed that all granules, except the TIMP-1 vesicle, appeared to be calcium regulated. This suggests that the regulation of proMMP-9 release is not exclusively via calcium and that TIMP-1 vesicle release is not calcium regulated. Whereas most granules were shown to be associated with microtubule-like structures, the TIMP-1 vesicle and proMMP-9-containing granules were shown to associate with two morphologically different cytoskeletal elements, neither resembling actin nor tubulin. These elements, and the release of the TIMP-1 vesicle and proMMP-9-containing granules, need to be studied further, but results achieved to date may explain the observed differential mode of release of TIMP-1 relative to proMMP-9. The proMMP-9-binding and inhibitory capacity of a 66 kDa high molecular mass form of TIMP-1 was demonstrated in PMNL homogenates and plasma using western ligand blots and a novel reverse zymography method. The role and relevance of this form remains unknown as does the relevance and potential role of proMMP-9ffIMP-1 complexes seen during isolation procedures. The proMMP-9ffIMP-1 complex may occur in vivo, as evidenced by immunolocalisation studies, and, together with TIMP-1 released from its own discrete vesicle population, may be responsible for the fine regulation of extracellular proteolysis. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2002.
10

Caracterização das alterações laboratoriais e histopatológicas associadas à obstrução uretral experimentalmente induzida em ratos /

Costa, Hugo Leonardo Riani. January 2008 (has links)
Orientador: Regina K. Takahira / Banca: Raimundo de Souza Lopes / Banca: André Marcelo Conceição Meneses / Resumo: A obstrução uretral é uma emergência clínica freqüente no atendimento de pequenos animais. Com a evolução do quadro, ocorre parada na filtração glomerular e, consequentemente, desenvolvem-se várias alterações nos equilíbrios hídrico, eletrolítico e ácido-básico, além do acúmulo de metabólitos nitrogenados e toxinas orgânicas. Podem ocorrer modificações histopatológicas nos rins e na bexiga. Objetivou-se, neste estudo, caracterizar prospectivamente as alterações laboratoriais e histopatológicas de ratos apresentando obstrução uretral. Para tanto, foram utilizados 21 ratos Wistar com obstrução uretral induzida. Foram realizados os seguintes exames: hemogasometria venosa e determinação dos níveis de uréia, creatinina, sódio, potássio, cloreto, cálcio e fósforo. As avaliações foram repetidas a cada 8 horas durante 24 horas. Após esse período os animais foram eutanasiados e as bexigas e os rins enviados para exame histopatológico. Entre os exames bioquímicos, foram observadas elevações estatisticamente significativas nos níveis de uréia, creatinina, fósforo, magnésio e potássio, e diminuição nos níveis de cloreto. Com relação à hemogasometria, houve diferença estatisticamente significativa entre os valores de pH, PO2, PCO2, excesso de base, saturação de oxigênio e lactato. O exame histopatológico renal revelou a presença de alterações tubulares e glomerulares, enquanto a análise histopatológica das bexigas demonstrou a presença de hemorragia, separação de fibras musculares e infiltrado inflamatório. Conclui-se que a obstrução uretral provoca alterações que podem ser detectadas nos exames laboratoriais, sendo as mesmas agravadas no decorrer do tempo. Além disso, a persistência durante 24 horas é capaz de levar a alterações morfológicas no trato urinário. / Abstract: Urethral obstruction is a frequent emergency in Veterinary clinics. The persistent urethral obstruction leads to blockage of renal filtration, resulting in several alterations in fluid, electrolyte and acid-base balance, besides the accumulation of nitrogenous metabolic products and organic toxins. Histopathological changes may occur in the kidneys and urinary bladder. Thus, this study aimed to prospectively characterize renal and vesical histopathological alterations in rats due to urethral obstruction. Twenty-one male Wistar rats (Rattus norvegicus) with experimental urethral obstruction were included in the study. Venous gasometry and determination of urea, creatinine, sodium, potassium, chloride, calcium and phosporus were performed. The avaliations were repeted each 8 hours during 24 hours. After that period, the animals were euthanatized for the collection of kidneys and bladder fragments to the histopathological exam. Biochemistry exams demonstrated statiscally significant elevations for the levels of urea, creatinine, phosporus, magnesium and potassium, and a decrease for the levels of chloride. Results of gasometry also demonstrated statiscally significant changes for pH, PO2, PCO2, base excess, oxygen saturation and lactate values. Histopathology analysis revealed kidney alterations in tubular and glomerular elements. The most important alterations found in urinary bladders were transmural hemorrhage, separation of muscle fibers and neutrophilic inflammatory infiltrate. Complete urethral obstruction induces important changes that can be detected by laboratorial exams, and the alterations worsen with the course of time. Besides that, the persistent obstruction during 24 hours is able to cause morphological changes in the kidneys and urinary bladder, which can be detected using histopathological exam. / Mestre

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