Resumo: A energia por microondas tem sido recomendada para desinfetar próteses totais. Entretanto, os ciclos de desinfecção podem afetar a estabilidade dimensional das bases de próteses. Assim, o objetivo deste estudo clínico foi avaliar o efeito de dois protocolos de desinfecção por microondas na estabilidade dimensional de próteses totais, analisando a estabilidade dimensional linear, o percentual de força oclusal obtido a partir dos contatos oclusais cêntricos e as ocorrências clínicas. Foram confeccionadas próteses totais para quarenta pacientes totalmente edêntulos. Todas as próteses foram prensadas com a resina Lucitone 550. Após a colocação, aguardou-se 30 dias para submeter as próteses às condições experimentais como a seguir: Grupo 1 (G1)- 20 pacientes tiveram as próteses maxilares submetidas a desinfecção por microondas (650 W / 3 minutos), uma vez por semana, durante 4 semanas. Grupo 2 (G2)- 20 pacientes tiveram as próteses maxilares submetidas a desinfecção por microondas (650 W / 3 minutos), três vezes por semana, durante 4 semanas. Para avaliar a estabilidade dimensional linear, mensurações foram realizadas entre quatro pontos de referência (primeiros pré-molares e segundos molares bilateralmente) antes da primeira desinfecção por microondas (baseline) e após cada semana de desinfecção. Um software foi utilizado para mensurar as distâncias entre os pontos de referência a partir de fotografias padronizadas. As dimensões médiolateral e anteroposterior e a área foram calculadas. Os dados foram analisados pelos testes ANOVA two-way, Tukey e t-Student (_=0,05). O sistema T-Scan III foi utilizado para avaliar o percentual de força oclusal obtido a partir dos contatos oclusais cêntricos. Os contatos oclusais cêntricos foram capturados, antes da primeira desinfecção por microondas (baseline) e após cada semana de desinfecção, por meio... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Microwave energy has been recommended for disinfecting complete dentures. However, the microwave disinfection cycles may affect the dimensional stability of denture bases. Therefore, the purpose of this clinical study was to evaluate the effect of two microwave disinfection protocols on the dimensional stability of complete dentures, analyzing the linear dimensional stability, the percent occlusal force obtained from centric occlusal contacts and the clinical events. Complete dentures were constructed for forty totally edentulous patients. All dentures were conventionally processed with heat-polymerized acrylic resin (Lucitone 550). After insertion of complete dentures, it was expected 30 days to submit the dentures to the experimental conditions as follows: Group 1 (G1)- 20 patients had the maxillary complete dentures submitted to microwave disinfection (650 W/ 3 minutes), once a week, for four weeks. Group 2 (G2)- 20 patients had the maxillary complete dentures submitted to microwave disinfection (650 W/ 3 minutes), three times a week, for four weeks. To evaluate the linear dimensional stability, measurements were taken across four reference points (first premolars and second molars bilaterally) before of first microwave disinfection (baseline) and after each week of disinfection. Software was used to measure distances between reference points from standardized photographs. Mediolateral and anteroposterior dimensions and area between reference points were calculated. The dimensional data obtained were analyzed using two-way ANOVA, Tukey test and t test (_ = .05). To evaluate the percent occlusal force obtained from centric occlusal contacts, the T-Scan III system was used. The centric occlusal contacts were measured, before of first microwave disinfection (baseline) and after each week of disinfection, using pressure-sensitive sheets and studied in T-Scan software. The occlusal data... (Complete abstract click electronic access below) / Orientador: Eunice Teresinha Giampaolo / Coorientador: Marco Antonio Compagnoni / Banca: Raphael Freitas de Souza / Banca: Claudia Helena Lovato da Silva / Banca: Ana Claudia Pavarina / Banca: João Neudenir Arioli Filho / Doutor

This thesis is a contribution to the formalisation of the notion of an agent within the class of finite multivariate Markov chains. In accordance with the literature agents are are seen as entities that act, perceive, and are goaldirected. We present a new measure that can be used to identify entities (called i-entities). The intuition behind this is that entities are spatiotemporal patterns for which every part makes every other part more probable. The measure, complete local integration (CLI), is formally investigated within the more general setting of Bayesian networks. It is based on the specific local integration (SLI) which is measured with respect to a partition. CLI is the minimum value of SLI over all partitions. Upper bounds are constructively proven and a possible lower bound is proposed. We also prove a theorem that shows that completely locally integrated spatiotemporal patterns occur as blocks in specific partitions of the global trajectory. Conversely we can identify partitions of global trajectories for which every block is completely locally integrated. These global partitions are the finest partitions that achieve a SLI less or equal to their own SLI. We also establish the transformation behaviour of SLI under permutations of the nodes in the Bayesian network. We then go on to present three conditions on general definitions of entities. These are most prominently not fulfilled by sets of random variables i.e. the perception-action loop, which is often used to model agents, is too restrictive a setting. We instead propose that any general entity definition should in effect specify a subset of the set of all spatiotemporal patterns of a given multivariate Markov chain. Any such definition will then define what we call an entity set. The set of all completely locally integrated spatiotemporal patterns is one example of such a set. Importantly the perception-action loop also naturally induces such an entity set. We then propose formal definitions of actions and perceptions for arbitrary entity sets. We show that these are generalisations of notions defined for the perception-action loop by plugging the entity-set of the perception-action loop into our definitions. We also clearly state the properties that general entity-sets have but the perception-action loop entity set does not. This elucidates in what way we are generalising the perception-action loop. Finally we look at some very simple examples of bivariate Markov chains. We present the disintegration hierarchy, explain it via symmetries, and calculate the i-entities. Then we apply our definitions of perception and action to these i-entities.

Syftet med studien är att få en fördjupad insikt i vad lärandet av sångtekniken neutral utan luftinnebär. För att undersöka detta har jag under en fem veckors period övat neutral utan lufti 20-30 minuter, tre dagar i veckan. Under och efter övningspassen har loggbok använts för att dokumentera nya upplevelser av lärandeprocessen och utöver detta har även 5 videodokumentationer av övningspass gjorts. I denna studie utgörs den teoretiska utgångspunkten av ett designteoretiskt perspektiv med fokus på vilka semiotiska resurser som används i lärandet. Studiens frågeställningar är: Vilka teckenskapande resurser används vid inlärningen av den nya funktionen så kalladneutral utan luft? Hur används dessa resurser? Resultatet visar att de viktigaste resurserna är kroppen och de sångtekniska elementen. Dessa resurser används för att åstadkomma ett stadigt och önskvärt resultat på ett oskadligt vis. I diskussionskapitlet diskuterar jag de resurser som krävts i inlärningen av sångtekniken neutral utan luft. / The purpose of the study is to gain a deepened insight in what the learning of the singing technique neutral without aircan mean. To study this I have practiced neutral without air20-30 minutes per day, three times a week over a five weeks’ period. During and after every practice session I have written a log to document new personal experiences of the learning process. In addition to this I have documented five practice sessions with a video camera. The theoretical perspective of this study is design theory with focus on the semiotic resources used during the learning process. The study’s research questions are: Which semiotic resources are used during learning the song technique known as neutral without air? How are these resources used? The results show that the most central resources that were used were the body and the vocal elements of the technique. These resources are used to achieve a steady and desirable result in an innocuously manner. In the discussion, I describe the necessary resources for the learning process of the song techniqueneutral without air.

O peptídeo liberador da gastrina (GRP), homólogo mamífero da bombesina (BN), é um neuropeptídeo com múltiplas ações biológicas, incluindo um importante papel na regulação da resposta imunológica e inflamatória. Está presente em altas concentrações no líquido sinovial de pacientes com artrite reumatóide (AR), paralelamente com os níveis de interleucina-6 (IL-6) e fator de necrose tumoral (TNF). RC-3095 é um antagonista sintético do receptor da BN/GRP que demonstrou modular a liberação de citocinas pró-inflamatórias (TNF e IL-1 ) e diminuir o infiltrado inflamatório em modelos experimentais de sepse. Este estudo avaliou os efeitos do RC-3095 nos parâmetros clínicos, histopatológicos e de mediadores inflamatórios na artrite induzida por adjuvante completo de Freund (CFA) em ratos. A artrite foi induzida por uma injeção de CFA junto a superfície subplantar da pata esquerda de ratos machos de Wistar. Os animais foram divididos 4 grupos: grupo controle, controle injetado com veículo, grupo placebo (administrado salina subcutaneamente 50ml/kg, uma vez ao dia por 8 dias após estabelecimento do modelo), grupo tratado (0.3 mg/kg de RC-3095 subcutaneamente, uma vez ao dia por 8 dias após a indução). A avaliação clínica foi acompanhada diariamente, de acordo com um escore do edema para as patas posteriores. O peptídeo liberador da gastrina (GRP), homólogo mamífero da bombesina (BN), é um neuropeptídeo com múltiplas ações biológicas, incluindo um importante papel na regulação da resposta imunológica e inflamatória. Está presente em altas concentrações no líquido sinovial de pacientes com artrite reumatóide (AR), paralelamente com os níveis de interleucina-6 (IL-6) e fator de necrose tumoral (TNF). RC-3095 é um antagonista sintético do receptor da BN/GRP que demonstrou modular a liberação de citocinas pró-inflamatórias (TNF e IL-1 ) e diminuir o infiltrado inflamatório em modelos experimentais de sepse. Este estudo avaliou os efeitos do RC-3095 nos parâmetros clínicos, histopatológicos e de mediadores inflamatórios na artrite induzida por adjuvante completo de Freund (CFA) em ratos. A artrite foi induzida por uma injeção de CFA junto a superfície subplantar da pata esquerda de ratos machos de Wistar. Os animais foram divididos 4 grupos: grupo controle, controle injetado com veículo, grupo placebo (administrado salina subcutaneamente 50ml/kg, uma vez ao dia por 8 dias após estabelecimento do modelo), grupo tratado (0.3 mg/kg de RC-3095 subcutaneamente, uma vez ao dia por 8 dias após a indução). A avaliação clínica foi acompanhada diariamente, de acordo com um escore do edema para as patas posteriores. / Background: Recently, targeting of neuropeptides has been suggested to have potential therapeutic effects in RA. The gastrin-releasing peptide (GRP) is the homologous mammalian of the bombesin (BN), and its receptor signaling is involved in several functions, including cancer growth, immune cell regulation and inflammatory conditions, and has been found in RA synovial fluid in concentrations that correlate with IL-6 and TNF. RC-3095 is the antagonist of the GRP receptor, and has been demonstrated to modulate the release of proinflammatory cytokines (TNF and IL-1 ) by activated macrophages, leading to decreased inflammatory infiltration. Objective: To determine the effects of RC-3095 in clinical and histopathologic parameters and inflammatory mediators on complete Freund´s adjuvant-induced arthritis. Methods: The arthritis was induced by injection of complete Freund´s adjuvant (CFA) into the left hind footpad in males of Wistar rats. The animals were divided into control, vehicle injected control, placebo group (saline subcutaneously 50ml/kg, once daily for 8 days after modeling), treatment group (0.3 mg/kg of RC-3095 subcutaneously, once daily for 8 days after induction). Clinical evaluation was accomplished daily, through scoring of the paw edema. The animals were sacrificed 15 days after induction for collection of hind foot joints for histology. We used a histological scoring system which was previously described, and interferon (INF)-g, Interleukin (IL)-1 , tumor necrosis factor (TNF), interleukin (IL)-6 and interleukin (IL)-10 were measured by ELISA. Statistical analyses were compared by Student t-test to determine the difference between placebo and treatment groups for histological scores, and cytokines levels were compared using Mann-Whitney test (p<0.05). Results: There was significant inhibition of joint histological findings in the RC-3095 treated group, including synovial inflammatory infiltration(p<0,001), synovial hyperplasia (p=0.004), extension of pannus (p<0.001), synovial fibrosis (p<0.001), angiogenesis (p=0.033), cartilage (p 0.001) and bone erosion (p<0.001). IFN-g (p<0.001), IL-1 (p<0.001), TNF (p=0.003), IL-6 (p=0.048) and IL-10 (p=0.015) serum levels were significantly lower in the treated group. Paw swelling and subcutaneous inflammation, evaluated clinically, were not different between CFAinduced groups. Conclusions: RC-3095 was able to improve experimental arthritis, attenuate joint damage and decrease serum levels of IFN-g, IL-1 , TNF, IL-6 and IL-10. These data indicate that interference with GRP pathway is a potential new strategy for the treatment of RA that needs further investigational studies.

Introdução: O Transplante Alogênico de Célula Tronco Hematopoiética (TCTH alogênico) é um procedimento de alto potencial curativo para a Leucemia Mielóide Aguda (LMA), principalmente pelo efeito “graft versus leukemia” (GVL), que leva a redução do risco de recaída. Atualmente, os pacientes com LMA de risco intermediário são submetidos ao procedimento caso possuam doador aparentado. Pacientes sem doador aparentado disponível são submetidos a tratamento de consolidação com quimioterapia, com maior chance de recaída da doença. Acredita-se que os pacientes submetidos ao TCTH tenham maiores sobrevida global e livre de doença, a despeito das altas taxas de morbimortalidade. A classificação de risco é extremamente importante para escolha terapêutica pós remissão. Assim, a realização da pesquisa de marcadores moleculares, para refinar a estratificação prognóstica, tem importância especial no grupo de risco intermediário, complementando a avaliação citogenética, e auxiliando na decisão terapêutica, sendo cada vez mais necessária, apesar de não disponível em todos os centros. Material e métodos: Foram avaliados os pacientes com LMA de risco intermediário em primeira Remissão Completa (1RC) do Serviço de Hematologia e TCTH do Hospital de Clínicas de Porto Alegre do período de 01 de abril de 1999 a 01 de outubro de 2014, com pelo menos 1 ano de seguimento após o tratamento, através de revisão de prontuários. Os dados foram dispostos no programa Excel e posteriormente exportados para o programa SPSS v. 18.0 para análise estatística. Resultados: Foram avaliados 69 pacientes, sendo 45 pacientes submetidos a consolidação com quimioterapia (“QT”) e 24 submetidos a TCTH Alogênico (“TCTH Alogênico”). A média de idade do grupo “QT” foi de 47,8 anos e do grupo “TCTH Alogênico” foi de 35,5 anos, com diferença estatisticamente significativa (P<0,001). Não houve diferença na distribuição entre o sexo. A mediana de tempo de seguimento do grupo “QT” foi de 1,1 anos (intervalo interquartil de 0,4 a 2,5 ) e no grupo “TCTH Alogênico” foi de 2,7 anos (intervalo interquartil de 0,4 a 5,5), sem diferença estatisticamente significativa na distribuição dos tempos de seguimento entre os grupos (P=0,236). A sobrevida do grupo “QT” em 12 meses foi de 52,3% e no grupo “TCTH Alogênico” foi de 62,5%. Aos 24 meses, a sobrevida do grupo “QT” foi de 31,7% e no grupo “TCTH Alogênico” foi de 58,3% e em 5 anos de 21,1% e 53,8%, respectivamente. O teste do Long-Rank aponta uma diferença estatisticamente significativa nas sobrevidas entre os grupos após 5 anos, com Hazard Ratio (HR) para óbito de 2,2 (IC 95%: 1,1-4,2), P=0,027, porém ao ajustarmos a relação pela idade esta associação perde significância estatística (HR:1,6 IC95%:1 - 1,1; P=0,246) Discussão: Os dados evidenciaram melhor sobrevida no grupo submetido à TCTH alogênico, porém o grupo submetido ao procedimento apresentava média de idade menor. No entanto, apesar da perda da significância estatística, o HR corrigido para idade permanece maior para o grupo sem TCTH, o que pode dever-se ao “n” pequeno da amostra. Identificar quais pacientes terão benefício com TCTH torna-se cada vez mais um desafio. O uso de marcadores moleculares são importantes no refinamento da estratificação de risco do grupo de risco intermediário, podendo auxiliar nessa decisão. Além disso, com o advento da possibilidade de condicionamentos não mieloablativos como alternativa aos pacientes mais velhos e com escore de comorbidades pior e a melhor terapia de suporte, talvez possamos ser menos conservadores na indicação desse procedimento, identificando assim aqueles que poderão obter melhores resultados no tratamento de uma doença tão agressiva e grave. / Background: Allogeneic Hematopoietic Stem Cell Transplantation (allo-HSCT) is a high potentially curative procedure to Acute Myeloid Leukemia (AML), mainly by the “graft-versus-leukemia” (GVL) effect, which leads to reduced risk of relapse. Nowadays, intermediate risk AML patients are submitted to this procedure if a matched sibling donor is available. Patients without a sibling donor are submitted to consolidation with chemotherapy, with a greater chance of relapse. It is believed that patients submitted to allo-HSCT have a greater overall survival and disease-free survival, even though it presents high morbidity and mortality rates. Risk stratification is extremely important to post-remission treatment choice. Molecular markers research is especially important in intermediate risk group, complementing cytogenetic evaluation to a better prognostic stratification and, although it is still not available in all health centers, it is more and more necessary. Materials and Methods: We evaluated intermediate risk AML patients in first Complete Remission (CR1) at the Hematology Service and Bone Marrow Transplantation from Hospital de Clínicas de Porto Alegre from April 1st 1999 to October 1st 2014, and which had, at least, a one year follow-up after treatment, by conducting a medical record review. Data was inserted in Microsoft Excel 2010 spreadsheets and after exported to SPSS v. 18.0 to statistical analysis. Results: Among the 69 patients analyzed, 45 were submitted to consolidation with chemotherapy (Intermediate risk AML – non allo-HSCT) and 24 of then submitted to allo-HSCT (Intermediate risk AML – allo-HSCT). The average age of Intermediate risk AML – non allo-HSCT was 47.8 years old and Intermediate risk AML – allo-HSCT was 35.5 years old, with statistically significance difference (P<0,001). There was no difference regard sex of patients. The median follow-up in the Intermediate risk AML – non allo-HSCT was 1.1 years (interquartile rage of 0.4 to 2.5) and in the Intermediate risk AML – allo-HSCT was 2.7 years (interquartile rage of 0.4 to 5.5), with no statistically significance difference in follow-up time distribution between groups (P=0.236). Intermediate risk AML – non allo-HSCT survival in 12 months was 52.3% and in the Intermediate risk AML – allo-HSCT was 62.5%. In 24 months, Intermediate risk AML – non allo-HSCT survival was 31.7% and in Intermediate risk AML – allo HSCT survival was 58.3% and in 5 years it was 21.1% and 53.8% respectively. Long- Rank test indicates a statistically significant difference in survival between groups after 5 years, with hazard ratio (HR) for death of 2.2 (IC95% 1.1 – 4.2), P=0.027, but when we adjust the relation to age, this association loses statistical significance (HR:1.6 95%CI: 1 – 1.1; P=0.246). Discussion: Data showed a better survival rate to the group submitted to allo-HSCT, but the group presented a lower average age. However, despite de loss of statistical significance, Hazard Ratio (HR), adjusted to age remains higher to the non allo-HSCT group. It can be explained by the small number of the sample. Identifying which patients will benefit from allo-HSCT becomes increasingly challenging. The use of molecular markers are important in the refinement of risk stratification in intermediate risk group, assisting in the decision. Moreover, with the advent of the possibility of nonmyeloablative conditioning as an alternative to older patients and with worst rates of comorbidity, and the better supporting therapy, we may be less conservative in indicating this procedure, identifying the patients who may obtain better results during treatment of such aggressive and serious disease.

Dans nos travaux, nous avons considéré un processus de Lévy X avec une composante brownienne non nulle et dont la partie à sauts est un processus de Poisson composé. Nous avons supposé que la valeur d'une entreprise est modélisée par un processus stochastique de la forme V = Vo exp X et que cette entreprise est mise à défaut dès lors que sa valeur passe sous un certain seuil b déterminé de façon exogène et qui donc, est une donnée du problème. L'instant de défaut T est alors de la forme Tx pour x= ln(Vo) ln((b) où x> 0, Tx = inf{t 2:0: X, 2:x}. Dans un premier temps, nous supposons que des agents observant la valeur V des ac­tifs de la firme souhaitent connaître le comportement de l'instant de défaut. Dans ce modèle, au chapitre 2, nous avons étudié d'une part la régularité de la densité de la loi de l'instant de défaut. D'autre part, nous avons étudié la loi conjointe de l'instant de défaut, de l'overshoot et de l'undershoot. Au chapitre 3, nous avons obtenu une équation à valeurs mesures dont le quadriplet formé par la variable aléatoire X,, le su­ premum du processus X à l'instant t, le supremum du processus X au dernier instant de saut avant l'instant t et le dernier instant de saut à l'instant t est solution au seris faible, puis une équation dont ce quadriplet est une solution forte. Dans un second temps, au chapitre 4, nous avons supposé que des investisseurs souhaitant détenir une part de cette entreprise ne disposent pas de l'information complète. Ils n'observent pas la valeur des actifs de la firme V, mais sa valeur bruitée. Leur information est modélisée par la filtration Ç = (Ç,, t 2: 0) engendrée par cette observation. Dans ce modèle, nous avons montré que la loi conditionnelle de l'instant de défaut sachant la tribu Ç, admet une densité par rapport à la mesure de Lebesgue et obtenu une équation de Volttera dont cette densité est solution. Cette connaissance permet aux investisseurs de prévoir au vu de leur information, quand est-ce que l'instant de défaut va intervenir après l'instant t. Nous avons complété ce travail par des simulations numériques. / In this Ph.D thesis, we consider a jump-diffusion process which the diffusion part is a drifted Brownian motion and the jump part is a compound Poisson process. We assume that a firm value is modelling by a stochastic process V = V0 exp-X. This firm goes to default whenever its value is below a specified tlrreshold b which is exo­ genously determined. For x = ln(Vo) - ln(b) > 0, the default time is of the form Tx = inf{t 2:0: X, 2: x}. First, we suppose that agents observe perfectly the firm value. In this mode, we sho­ wed in chapter 2 that the density of the default time is continuons, then study the joint law of the default time, overshoot an undershoot. We obtained in chapter 3 a valued measure differentia equation which the solution is the quadruplet formed by the random variable X,, the running supremum x; of X at time t, the supremum of X at the last jump time before t and the last jump time before t. Secondly, we assume that investors wishing detain a part of the firm can not observe the firm value. They observe a noisy value of the firm and their information is madel­ ling by the filtration g = (9,,t 2: 0) generated by their observation. In this mode, we have shown that the conditional density of Tx with respect to Ç has a density which is solution of one stochastic integral-differentia equation The knowledge of this density allows investors to predict the default time after time t. This second part is the chapter 4.

Depuis plusieurs années, on assiste à la croissance exponentielle des cas de plagiat non seulement dans les arts, mais aussi dans la recherche scientifique. La réalité veut qu’en l’absence d’un spécialiste de la littérature dans les débats à la barre pour trier le bon grain de l’ivraie, le juge se trouve très souvent obligé de jouer le rôle du spécialiste à la fois du droit et de la littérature. C’est donc dans ce contexte particulier, que se situe ce travail de recherche. L’objectif est de partir de l’analyse de quatre biographies, de deux romans et de trois décisions de justice pour essayer de pallier les insuffisances d’une « approche juridique » de la littérature et proposer une grille d’analyse littéraire systématisée. Cette grille d’analyse résulte de la conjugaison de deux processus : l’analyse manuelle et celle assistée par ordinateur. En effet, avec pour cadre général l’analyse de l’œuvre intégrale, l’analyse manuelle se combine avec la textométrie. faite à l’aide de deux logiciels : TXM conçu par le laboratoire ICAR de l’ENS de Lyon et Hyperbase conçu par le Professeur Étienne Brunet de l’université de Nice. C’est à l’issue de toutes ces investigations que le spécialiste de la littérature devrait émettre un avis motivé dont le tribunal devrait se servir pour dire le droit et... rendre justice / For several years now, there has been an exponential growth in cases of plagiarism not only in the arts, but in scientific research as well. Reality warrants that in the absence of a specialist in literature in court to separate the gold from the dross, the judge is often compelled to play the rote of both a specialist in law and in literature. It is against this backdrop that this research work is inspired. The aim is to analyze four biographies, two novels and three court rulings in an attempt to address the shortcomings of “legal approach” to literature and propose a systematized literary analytical framework. This analytical framework is the result of combining two processes : the manual process and the computer-assisted one. As a matter of fact, against a general framework of analyzing the complete works, manual analysis is combined with textometry, carried out using two softwares: TXM designed by the iCAR laboratory of ENS Lyon and Hyperbase designed by Professor Etienne Brunet of Nice University. It is after all these investigations that the literature specialist may issue a reasoned opinion that the court may use to say what the law is and... mete out justice

The topic of my diploma thesis draws on my bachelor thesis, which described combustion products of selected substances in the complete combustion. The diploma thesis focused on the incomplete combustion in the same substances to make a comparison possible. In the chapter on the substances selected for burning, the substances were described theoretically first. Further, the burning was carried out, the products that came into being were collected into collection bags and sent to the laboratory of the Institute of Population Protection in Lázně Bohdaneč to be analysed. After receiving the records all possible effects on human health were carried out, and the results were compared with those of the bachelor thesis. The analyses showed that carbon monoxide and sulphur dioxide appeared in all analyses, while the remaining substances differed to a minimum extent, however, they are in much higher concentrations than in the perfect combustion. I added two more substances that had not been in my bachelor thesis to those that were burnt, but in the course of two years their application became a common practice, and they play an increasingly more significant role in our life. The task of the chapter dealing with the detection technique was to find out and describe the possibilities of the commander in detecting the combustion products directly on the spot of the actual operation in the shortest possible time, to enable him to propose the protection of the firemen, and possibly to establish the possibilities of taking samples, transport to the laboratory, conducting a laboratory analysis and establishing the approximate time when he could obtain the first sampling results. All of this was focused on the possibilities of the Fire Rescue Service of the Region of South Bohemia (HZS JčK). The next chapter concerned their colleagues of the Fire Rescue Service of the Region of South Bohemia in České Budějovice, in which a questionnaire investigation was focused on them, identical with that of my bachelor thesis. As I had succeeded in including the results of my bachelor thesis into a training course, I wondered whether the knowledge would improve or not. After a comparison with the results from my bachelor thesis with the new ones the conclusions turned out to be favourable, and suggested in which way the training courses - not only for the professional units - should proceed. The results of the new questionnaire investigation, which showed an improvement, should form a basis of a campaign focusing on the non-professional public, which should be acquainted with what they breathe in the event that they burn vast variety of waste. I believe that results would be achieved even here in the form of a deeper knowledge and a better quality of the air we breathe.

The dissertation deals with art expression of a family by pupils of a primary school. Its goal is to compare this art expression of a family by pupils from complete and incomplete families. The theoretical part is focused on family issues and its relationships in connection with their importance for young children´s age. Attention will be also paid to a mental and visual develompent of children of that age. The practical part is focused on examination and comparison of the portrayal of a family by young children´s age from complete and incomplete families. The attention will be also paid to the reflection of a family situation on pupils and their art production.

O peptídeo liberador da gastrina (GRP), homólogo mamífero da bombesina (BN), é um neuropeptídeo com múltiplas ações biológicas, incluindo um importante papel na regulação da resposta imunológica e inflamatória. Está presente em altas concentrações no líquido sinovial de pacientes com artrite reumatóide (AR), paralelamente com os níveis de interleucina-6 (IL-6) e fator de necrose tumoral (TNF). RC-3095 é um antagonista sintético do receptor da BN/GRP que demonstrou modular a liberação de citocinas pró-inflamatórias (TNF e IL-1 ) e diminuir o infiltrado inflamatório em modelos experimentais de sepse. Este estudo avaliou os efeitos do RC-3095 nos parâmetros clínicos, histopatológicos e de mediadores inflamatórios na artrite induzida por adjuvante completo de Freund (CFA) em ratos. A artrite foi induzida por uma injeção de CFA junto a superfície subplantar da pata esquerda de ratos machos de Wistar. Os animais foram divididos 4 grupos: grupo controle, controle injetado com veículo, grupo placebo (administrado salina subcutaneamente 50ml/kg, uma vez ao dia por 8 dias após estabelecimento do modelo), grupo tratado (0.3 mg/kg de RC-3095 subcutaneamente, uma vez ao dia por 8 dias após a indução). A avaliação clínica foi acompanhada diariamente, de acordo com um escore do edema para as patas posteriores. O peptídeo liberador da gastrina (GRP), homólogo mamífero da bombesina (BN), é um neuropeptídeo com múltiplas ações biológicas, incluindo um importante papel na regulação da resposta imunológica e inflamatória. Está presente em altas concentrações no líquido sinovial de pacientes com artrite reumatóide (AR), paralelamente com os níveis de interleucina-6 (IL-6) e fator de necrose tumoral (TNF). RC-3095 é um antagonista sintético do receptor da BN/GRP que demonstrou modular a liberação de citocinas pró-inflamatórias (TNF e IL-1 ) e diminuir o infiltrado inflamatório em modelos experimentais de sepse. Este estudo avaliou os efeitos do RC-3095 nos parâmetros clínicos, histopatológicos e de mediadores inflamatórios na artrite induzida por adjuvante completo de Freund (CFA) em ratos. A artrite foi induzida por uma injeção de CFA junto a superfície subplantar da pata esquerda de ratos machos de Wistar. Os animais foram divididos 4 grupos: grupo controle, controle injetado com veículo, grupo placebo (administrado salina subcutaneamente 50ml/kg, uma vez ao dia por 8 dias após estabelecimento do modelo), grupo tratado (0.3 mg/kg de RC-3095 subcutaneamente, uma vez ao dia por 8 dias após a indução). A avaliação clínica foi acompanhada diariamente, de acordo com um escore do edema para as patas posteriores. / Background: Recently, targeting of neuropeptides has been suggested to have potential therapeutic effects in RA. The gastrin-releasing peptide (GRP) is the homologous mammalian of the bombesin (BN), and its receptor signaling is involved in several functions, including cancer growth, immune cell regulation and inflammatory conditions, and has been found in RA synovial fluid in concentrations that correlate with IL-6 and TNF. RC-3095 is the antagonist of the GRP receptor, and has been demonstrated to modulate the release of proinflammatory cytokines (TNF and IL-1 ) by activated macrophages, leading to decreased inflammatory infiltration. Objective: To determine the effects of RC-3095 in clinical and histopathologic parameters and inflammatory mediators on complete Freund´s adjuvant-induced arthritis. Methods: The arthritis was induced by injection of complete Freund´s adjuvant (CFA) into the left hind footpad in males of Wistar rats. The animals were divided into control, vehicle injected control, placebo group (saline subcutaneously 50ml/kg, once daily for 8 days after modeling), treatment group (0.3 mg/kg of RC-3095 subcutaneously, once daily for 8 days after induction). Clinical evaluation was accomplished daily, through scoring of the paw edema. The animals were sacrificed 15 days after induction for collection of hind foot joints for histology. We used a histological scoring system which was previously described, and interferon (INF)-g, Interleukin (IL)-1 , tumor necrosis factor (TNF), interleukin (IL)-6 and interleukin (IL)-10 were measured by ELISA. Statistical analyses were compared by Student t-test to determine the difference between placebo and treatment groups for histological scores, and cytokines levels were compared using Mann-Whitney test (p<0.05). Results: There was significant inhibition of joint histological findings in the RC-3095 treated group, including synovial inflammatory infiltration(p<0,001), synovial hyperplasia (p=0.004), extension of pannus (p<0.001), synovial fibrosis (p<0.001), angiogenesis (p=0.033), cartilage (p 0.001) and bone erosion (p<0.001). IFN-g (p<0.001), IL-1 (p<0.001), TNF (p=0.003), IL-6 (p=0.048) and IL-10 (p=0.015) serum levels were significantly lower in the treated group. Paw swelling and subcutaneous inflammation, evaluated clinically, were not different between CFAinduced groups. Conclusions: RC-3095 was able to improve experimental arthritis, attenuate joint damage and decrease serum levels of IFN-g, IL-1 , TNF, IL-6 and IL-10. These data indicate that interference with GRP pathway is a potential new strategy for the treatment of RA that needs further investigational studies.