Global ETD Search

71	Development Of Efficient Modeling Methodologies Of Adhesively Bonded Joints For Crash Simulations Sureshrao, Malvade Indrajit 07 1900 (has links) In this thesis, a new modeling methodology applicable to adhesively bonded joints for crash simulations is presented. Using this approach, adhesive joints can be modeled without using minute solid elements thus reducing the size of the model. Moreover, coarse mesh can be used for substrates in the overlap region of a joint. Both of these improvements together yield significant reduction in simulation run times in crash analysis when compared to solid element representation of adhesive. The modeling can also capture effects of strain rate for a given ambient temperature. In order to develop the efficient modeling procedure mentioned above, experimental, analytical and numerical studies have been carried out. Mechanical behaviors of adhesively bonded joints are studied with the help of double lap shear (DLS) coupon tests conducted at different extension rates and temperatures. The joint specimens are made from dual-phase (DP) steel coupons bonded with epoxy resin. Tests are also carried out to ascertain the behaviors of these component materials at different extension rates and temperatures. A new semi-analytical solution procedure is developed considering material nonlinearity to predict mechanical behaviors of adhesively bonded DLS joints. The joint behaviors using the semi-analytical approach are predicted separately using the Von Mises and exponent Drucker-Prager yield criteria. The predicted force versus extension curves using semi-analytical solution are compared with test results. It is also hypothesized here that, the semi-analytical solution procedure can be used as a base to develop efficient modeling procedures of adhesively bonded joints in FEA. In finite element analysis, both adhesive and substrates are modeled as elastic-plastic materials. It is shown that the shell-solid model of the DLS joint, in which substrates are modeled using shell elements and adhesive is modeled using solid elements, can accurately predict the mechanical behavior of the joint. Both exponent Drucker-Prager and Von Mises material models in ABAQUS are used to calculate force versus extension curves. Numerical and experimental forces versus extension curves are compared. A new methodology for efficient modeling of adhesively bonded joints in LS-DYNA using equivalent material properties in the joint overlap region is proposed. Various models using this methodology are assessed by comparing their results with shell-solid model and test results. Finally, it is also shown that strain rate effects can be included in the efficient modeling approach. Bonded Joints Joints - Simulation Adhesive Joints - Modeling Double Lap Shear Joints- Modeling Crash Simulation DLS Joints Manufacturing Engineering
72	Metody rozptylu světla a kalorimetrie ve studiu systémů hyaluronan-albumin / Study of hyaluronan-albumin systems using light scattering methods and calorimetry Sereda, Alena January 2016 (has links) This thesis, which is a continuation of the previous Bachelor thesis, is dedicated to the study of polyelectrolyte complexes between hyaluronan (HA) and bovine serum albumin (BSA). Interactions between HA and BSA and a formation of complexes were studied by SEC-MALLS method, where a molar mass, a root mean square (rms) radius, a hydronamic radius and an intrinsic viscosity of particles of the system were defined. Furthermore the interactions were studied by calorimetric measurement ITC, where thermodynamic character of complex formation was determined. Additionally, dynamic and electrophoretic light scattering methods (DLS and ELS) were applied, where hydrodynamic radius and a value of the zeta potential were defined. Also the effect of higher temperature on the character and complex formation was examined by SEC-MALLS and ITC measurements. The interactions were confirmed at any of the used media, but with different efficiency. It was also proved that complexes become smaller in their radii and viscosity with growing BSA concentration. Furthermore it was proved, that the high ionic strength hinders surface charges of HA and BSA molecules and minimizes their mutual interactions. At the higher ionic strength the radii of the complexes, their intrinsic viscosity and zeta potential are increased. The higher temperature has only minimal effect on the formation of the complexes.
73	Stabilita systémů pro řízené uvolňování léčiv na bázi plastifikovaného škrobu / Stability of controlled drug release systems based on plasticized starch Zhukouskaya, Hanna January 2022 (has links) The thesis is focused on the research of stability of controlled drug release systems based on a blend of plasticized starch/polycaprolactone (TPS/PCL) that served as a carrier. Antibiotic vancomycin was used as a model drug, and its release from TPS/PCL pellets into aqueous environment was followed by UV-spectroscopy and the obtained time dependences were treated by a simple kinetic model. Moreover, the simultaneous release of starch particles to the surrounding liquid phase was studied by static and dynamic light scattering as well as transmission electron microscopy (TEM) in order to obtain information on the stability of biodegradable matrix and on the structure of the products of the pellet decomposition on a nanoscale level. Key words: vancomycin, starch, drug delivery system, polycaprolactone (PCL), particle release, dynamic light scattering (DLS), static light scattering (SLS)
74	Cardiotonic Steroids Down-Regulate Sodium Hydrogen Exchanger Expression in the Proximal Tubule Cells Oweis, Shadi 01 September 2010 (has links) No description available. Biomedical Research NHE3 Nephron Kidney: Natriuresis Hypertension Sodium Potassium Salt Ouabain Digoxin DLS CTS Cardiotonic Steroids Diuretic Sodium Hydrogen Exchanger Rostafuroxin Proximal Tubules Canrenone Src PI3K LLCPK1 Na/K ATPase Sodium Pump
75	Vesicle-Protein Diffusion and Interaction Study Using Time Resolved Fluorescence Correlation Spectroscopy Rouhvand, Bahar January 2017 (has links) No description available. Physical Chemistry Biochemistry Biophysics Molecular Biology Molecular Chemistry Scientific Imaging Fluorescence spectroscopy DLS Vesicle FITC Fluorescence Correlation function Cross correlation Auto correlation Lipid bilayer Binding Double-stranded DNA Lipid-polymer interactions Brownian diffusion Lipid mobility
76	Mechanistic insights into the stabilisation of biopharmaceuticals using glycine derivatives : the effect of glycine derivatives on the crystallisation, physical properties and behaviour of commonly used excipients to stabilise antigens, adjuvants and proteins in the solid state Bright, Andrew G. January 2015 (has links) This dissertation has focused on studying the effect of four glycine derivatives on the solid state properties of mannitol, glycine, and sucrose when freeze dried into blended mixtures. The primary goal was to assess their value for use in the stabilisation of vaccines in the solid state, by examining key physical and chemical characteristics, which have been documented to be beneficial to the stabilisation of biopharmaceutical formulations. The novel excipients; dimethyl glycine, and trimethyl glycine, were shown to retard the crystallisation and increase the overall glass transition temperature, of mannitol, when freeze dried as evidenced by DSC and Powder X-ray diffraction. Mannitol’s glass transition temperature increased from 100C to 12.650C and 13.610C when mixed with methyl-glycine and dimethyl glycine respectively. The glycine derivatives did not show the same effect on sucrose which remained amorphous regardless of the concentration of the other excipient. The different behaviour with the sucrose system was thought to be due to relatively high glass transition temperature of sucrose. Conversely glycine remained highly crystalline due it’s relatively low glass transition temperature. The novel excipient formulations were also assessed for their effect on the aggregation of the adjuvant aluminium hydroxide when freeze dried by Dynamic Light Scattering (DLS).The formulations containing the glycine derivatives all caused a decrease in the aggregation size of the adjuvant from ~26 μm, to 185 nm in the presence of methyl glycine. The effects of lysozyme and viral antigen on the adjuvants were also examined showing that the addition of the virus did not affect the size of the aggregates formed, however lysozyme showed significant decreases in the aggregates formed. Examination of the freezing method were also made showing that faster freezing rates produced smaller aggregates of the adjuvant. When investigating the rate at which the excipients lost water during secondary drying there was evidence of the formation of hydrates of glycine, trimethyl glycine, and mannitol has shown that the glycine derivatives have attributes which would be beneficial in stabilising vaccines in the solid state when freeze dried.
77	Mechanistic Insights into the Stabilisation of Biopharmaceuticals using Glycine Derivatives. The Effect of Glycine Derivatives on the Crystallisation, Physical Properties and Behaviour of Commonly used Excipients to Stabilise Antigens, Adjuvants and Proteins in the Solid State Bright, Andrew G. January 2015 (has links) This dissertation has focused on studying the effect of four glycine derivatives on the solid state properties of mannitol, glycine, and sucrose when freeze dried into blended mixtures. The primary goal was to assess their value for use in the stabilisation of vaccines in the solid state, by examining key physical and chemical characteristics, which have been documented to be beneficial to the stabilisation of biopharmaceutical formulations. The novel excipients; dimethyl glycine, and trimethyl glycine, were shown to retard the crystallisation and increase the overall glass transition temperature, of mannitol, when freeze dried as evidenced by DSC and Powder X-ray diffraction. Mannitol’s glass transition temperature increased from 100C to 12.650C and 13.610C when mixed with methyl-glycine and dimethyl glycine respectively. The glycine derivatives did not show the same effect on sucrose which remained amorphous regardless of the concentration of the other excipient. The different behaviour with the sucrose system was thought to be due to relatively high glass transition temperature of sucrose. Conversely glycine remained highly crystalline due it’s relatively low glass transition temperature. The novel excipient formulations were also assessed for their effect on the aggregation of the adjuvant aluminium hydroxide when freeze dried by Dynamic Light Scattering (DLS).The formulations containing the glycine derivatives all caused a decrease in the aggregation size of the adjuvant from ~26 μm, to 185 nm in the presence of methyl glycine. The effects of lysozyme and viral antigen on the adjuvants were also examined showing that the addition of the virus did not affect the size of the aggregates formed, however lysozyme showed significant decreases in the aggregates formed. Examination of the freezing method were also made showing that faster freezing rates produced smaller aggregates of the adjuvant. When investigating the rate at which the excipients lost water during secondary drying there was evidence of the formation of hydrates of glycine, trimethyl glycine, and mannitol has shown that the glycine derivatives have attributes which would be beneficial in stabilising vaccines in the solid state when freeze dried. / Stabilitech Ltd. and the Engineering and Physical Sciences Research Council (EPSRC). Freeze drying Glycine derivatives Differential scanning calorimetry (DSC) Powder X-Ray diffraction (P-XRD) Raman spectroscopy Crystallisation Water loss Vaccines Adjuvant Stabilisation Amorphous Gordon-Taylor equation Solubility parameters Karl Fischer Excipients Proteins DLS (Dynamic Light Scattering) Polymorphs Vaccines Formulation Mannitol Sucrose Biopharmaceuticals
78	Direct Detection of Aggregates in Turbid Colloidal Suspensions Ducay, Rey Nann Mark Abaque 13 August 2015 (has links) No description available. Analytical Chemistry Biochemistry Biomedical Engineering Biomedical Research Biophysics Chemical Engineering Chemistry Experiments Materials Science Medical Imaging Molecular Physics Molecules Nanoscience Nanotechnology Optics Organic Chemistry Physics Polymer Chemistry Polymers Scientific Imaging Nanoparticles nanoparticle aggregation empirical model gold nanoparticles polystyrene nanoparticles microspheres turbid media TIR total internal reflection biosensors highly-scattering nanoaggregation sensing DLS UV-Vis DLVO Zeta potential
79	Etude expérimentale de polyélectrolytes hydrophobes modèles Baigl, Damien 11 September 2003 (has links) (PDF) Un polyélectrolyte hydrophobe est un polymère portant des charges électriques lorsqu'il est en solution aqueuse et dont l'eau est un mauvais solvant pour le squelette. Cette thèse a pour objectif d'établir l'influence de la nature hydrophobe du squelette sur les propriétés physiques des polyélectrolytes. Pour cela, nous avons tout d'abord synthétisé une série de poly(styrène-\emph(co)-styrènesulfonate de sodium), appelés PSS, possédant des taux de charge $f$ variant entre 30\% et 100\% et comportant entre $N=120$ et $N=2520$ monomères par chaîne. Ces PSS sont caractérisés précisément et peuvent être considérés comme des polyélectrolytes hydrophobes modèles. Nous avons alors étudié leurs propriétés volumiques puis interfaciales.\\ \emph(1. Propriétés en volume.) Le taux de charge effectif de la chaîne unique est anormalement réduit par rapport au cas du polyélectrolyte hydrophile. D'autre part, les propriétés structurales ont été caractérisées par la diffusion des rayons X et la technique de la sonde colloïdale en microscopie à force atomique (AFM). La conformation des chaînes se fait ressentir puisque la longueur de corrélation varie comme $N^0C_p^(-\alpha)$ où $C_p$ est la concentration en polymère et $\alpha$ un exposant dépendant de $f$, décroissant de 1/2 ($f=100\%$) à 1/3 au voisinage de la limite de solubilité. Ces observations sont interprétées dans le cadre d'un modèle théorique prédisant la conformation de la chaîne isolée comme un collier de perles, constitué de globules denses (les perles) reliés deux à deux par un segment de chaîne étirée. La dynamique collective des chaînes, quant à elle, est très proche de celle des polyélectrolytes hydrophiles.\\ \emph(2. Propriétés aux interfaces.) Nous avons conçu une expérience permettant, par adsorption électrostatique ou hydrophobe, de fixer les chaînes de PSS sur une surface solide plane modifiée chimiquement. La couche de PSS adsorbée, immergée dans l'eau, est caractérisée $in~situ$ par ellipsométrie, réflectivité des rayons X haute énergie et microscopie à force atomique. Nous avons ainsi trouvé que la taille de perles varie entre 1 et 5 nm en fonction de $f$. Cette variation est en parfait accord avec les prédictions du modèle dit du collier de perles. Enfin, les polyélectrolytes hydrophobes s'adsorbent également aux interfaces hydrophobes, les perles, dans certains cas, s'étalant sur la surface. polyélectrolytes polyelectrolytes polyélectrolytes hydrophobes hydrophobic polyelectrolytes polystyrènesulfonate polystyrenesulfonate PSS AMAMPS SAXS SLS DLS diffusion des rayons X réflectivité des rayons X reflectivity X-ray scattering light scattering ellipsométrie ellipsometry adsorption structure structural properties interfaces surfaces traitement de surface silanisation sulfonation pression osmotique osmotic pressure charge effective effective charge fraction AFM sonde colloidale colloidal probe technique protéines proteins polypeptides polysaccharides Rayleigh instability instabilité de Rayleigh collier de perles pearl-necklace vésicules vesicles mouillage wetting FRAP microscopie fluorescence TEM cryo-TEM transmission electron microscopy RMN NMR

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