Global ETD Search

61	Characterization and Modeling of the Remodeling Process that Occurs in Modular Tissue Engineered Constructs Assembled Within Microfluidic Perfusion Chambers Khan, Omar 31 August 2011 (has links) Using a modular approach, a vascularized tissue construct is created by embedding functional cells within submillimeter-sized collagen cylinders (modules) while the outside surfaces are seeded with endothelial cells (EC). The void spaces created by randomly packing modules into a container form EC-lined perfusion channels. Upon implantation, the tissues are remodeled by and integrated into the host and experience, to some degree, immune and inflammatory responses. This work utilized microfluidic techniques to study and model the tissue remodeling in vitro in the absence of the host response. When the construct’s tortuous perfusion channels were reproduced in poly(dimethylsiloxane) microfluidic devices and lined with EC, perfusion at higher flow rates reduced EC activation and maintained the desired quiescent EC phenotype. When applying these results to collagen constructs, higher flow rates were not achievable due to the weak mechanical properties of collagen. To increase the collagen’s mechanical strength, a semi-synthetic collagen/poloxamine-methacrylate hydrogel was examined but due to its heterogeneous surface composition, there was inadequate EC attachment and the material was deemed unsuitable for this application. Proceeding with lower flow rates, tissues assembled within microfluidic perfusion chambers from EC-seeded collagen modules showed that over the course of 24 hours, perfusion did not significantly increase activation but instead increased KLF2 expression, a transcription factor involved in the establishment of EC quiescence, and disrupted VE-cadherin bonds between adjacent EC. However, after 1 week of perfusion, the majority of EC were lost. To ameliorate this loss, mesenchymal stromal cells (MSC) were embedded within the modules in order to take advantage of their anti-apoptotic and immunomodulation effects. The MSC temporarily mitigated the loss of the EC but did not prevent it. They did, however, take on a phenotype similar to smooth muscle cells and migrated towards the EC. Perhaps this indicates that the combination of EC, MSC and perfusion drives the creation and assembly of pseudo vessels. Together, the microfluidic techniques used in this study to assemble and perfuse modular tissues revealed new insights into the remodeling process and exposed critical issues surrounding the adaptation of the EC to the combination of perfusion, remodeling and changing flow fields. tissue engineering microfluidics perfusion endothelial cells mesenchymal stromal cells activation differentiation remodeling model collagen poloxamine activation shear stress disturbed flow vascular modular 0541 0542
62	Client-Therapist Interaction and Perceived Therapeutic Outcome Fogle, Joseph Edwin 12 1900 (has links) This study sought to determine the therapeutic effectiveness of client-therapist dyads in a residential treatment center for emotionally disturbed adolescents. The theories of George Kelly's personal construct psychology were utilized in assessing the dyadic relationship. The four elements investigated were organizational similarity, understanding, organizational congruency and predominant selves. The sample consisted of 140 dyads comprised of 10 adolescent boys and girls and 14 therapeutic staff of a residential treatment center in the southwest United States. Responses to Kelly's Role Construct Repertory Test were compared to four relational factors—parental/respect, identity, problem-solving, and sexual/affection—and two rating scales of client-therapist preference and ratings of therapeutic effectiveness. Contrary to expectations, as content similarity among dyads composed of clients and staff increased, there was not an increase in functional aspects of the therapy relationship. Possible mitigating factors may have been level of client disturbance and/or methodological issues relating to how organizational similarity was determined. Dyadic understanding was not found to be related to perceptions of the therapy relationship. This may be a function of adolescent of adolescent clients' need for independence and resistance to adult understanding and control. Therapy dyads with a moderate level of lateral or vertical organizational congruence were not found to be curvilinearly related to functional aspects of the therapy relationship. However, a weak linear relationship regarding client perceptions of the therapy relationship was noted on four measures. Several methodological recommendations related to the instruments used to determine therapeutic effectiveness and the means of eliciting personal constructs on the REP test. client-therapist dyads disturbed adolescents George Kelly personal construct psychology Kelly, George Alexander, 1905-1967. Psychotherapist and patient. Psychotherapy. Mentally ill children. Personal construct theory.
63	Particles in a linearly stratified fluid Khushal Ashok Bhatija (8081558) 04 December 2019 (has links) The settling of spherical and cylindrical particles in a linearly stratified fluid is investigated using experiments. The double-tank method is used to generate a linear stratification with a red colored dye homogeneously mixed in the heavy water tank. As a result of feeding the stratification using dyed heavy water, the concentration of dye varies with depth in the experiment tank. A powerful back-light and a digital camera are used to record the events. Assuming the concentration of dye is directly proportional to density of fluid, Beer-Lambert's law is used to generate a calibration between intensity of the light measured by the camera and density of the fluid. Using this calibration, density is evaluated in all the images captured. In the parameter space of this study, the spheres have three different wake patterns. The area of fluid disturbed by a suspension of spheres increases with <i>Re</i> and <i>Fr</i>. As a result, the amount of energy available for the mixing and the irreversible change of total potential energy in the system increases with <i>Re</i>, <i>Fr</i> and number of particles. Cylinders drag volumes of light fluid to larger depths in their wake than spheres and shed the light fluid in the form of vortices. This results in lower volumes of fluid perturbed by the cylinders. However, as the light fluid is dragged to larger depths, the amount of energy generated for mixing and the change in total potential energy of the system is higher. Spheres are thus more efficient in disturbing volumes of fluid but cylinders are more efficient in causing irreversible changes to the state of the system. Mechanical Engineering stratification irreversible change available potential energy total potential energy background potential energy volume disturbed suspension spheres cylinder experiments flow visualization dye
64	THE EXPERIENCE OF SENIOR STUDENT AFFAIRS ADMINISTRATORS MAKING PARENTAL NOTIFICATION DECISIONS ABOUT DISTURBED AND DISTURBED/DISTURBING STUDENTS Asimou, Holly M. 19 November 2013 (has links) No description available. Higher Education Higher Education Administration Mental Health College Students SSAOs Parental Involvement Mental Illness Disturbed and Disturbing Students Student Privacy FERPA ADA
65	Kontaktszenen. Narrative gestörter Wissenskommunikation Koch, Lars, Nitzke, Solvejg 06 June 2024 (has links) Das Verhältnis von Wissenschaft und Gesellschaft ist prekärer denn je. Je mehr Menschen durch breit verfügbare Bildungsangebote Zugang zu den Hochschulen erhalten, desto weiter scheint sich das Wissen, das an Universitäten im Bereich der Forschung produziert wird, den Zugriffen von ›Gesellschaft‹ zu entziehen. Dabei sollten doch gerade die großen Bildungsreformen gegen Ende des 19. und in der Mitte des 20. Jahrhunderts größere Schnittmengen zwischen Akademie und Gesellschaft erzeugen. ›Teilhabe‹ als Schlagwort gegenwärtiger Politik scheint aber genau das Gegenteil erreicht zu haben, nämlich eine Spaltung zwischen hochspezialisierter Forschung und Entwicklung einerseits und skeptisch bis renitent agierender Öffentlichkeit andererseits. Die (wissenschafts-)politische Reaktion ist deutlich: gefordert wird eine Popularisierungoffensive mit einem Mehr an Kommunikation, einem Mehr an Anschaulichkeit, einem Mehr an Personality¹. ... [Aus: einleitende Worte] info:eu-repo/classification/ddc/800 ddc:800
66	Design of a Bioreactor to Mimic Hemodynamic Shear Stresses on Endothelial Cells in Microfluidic Systems Lightstone, Noam S. 26 June 2014 (has links) The mechanisms behind cardiovascular disease (CVD) initiation and progression are not fully elucidated. It is hypothesized that blood flow patterns regulate endothelial cell (EC) function to affect the progression of CVDs. A system that subjects ECs to physiologically-relevant shear stress waveforms within microfluidic devices has not yet been demonstrated, despite the advantages associated with the use of these devices. In this work, a bioreactor was designed to fulfill this need. Waveforms from regions commonly affected by CVDs including were derived. Pump motion and fluid flow profiles were validated by actuator motion tracking, particle image velocimetry, and flowmeters. While several relevant waveforms were successfully replicated, physiological waveforms could not be produced at physiological frequencies owing to actuator velocity and accuracy limitations, as well as dampening effects in the system. Overall, this work lays the foundation for designing a system that provides insight into the role of shear stress in CVD pathogenesis. Endothelial cell Bioreactor Flow loop Cardiovascular disease CVD Waveform Microfluidics Microfluidic devices Hemodynamics EC Shear stress Shear Pump Actuator Mechanical engineering Biomedical engineering PIV Particle image velocimetry Fluid dynamics Fluid mechanics Pressure Model Syringe Peristaltic Blood Blood flow Wall shear stress Womersley number Womersley Purday Physiological Flowmeter Sinusoid Sinusoidal Disturbed Non-disturbed Undisturbed Oscillatory Pulsatile In vivo In vitro 0548 0541
67	Design of a Bioreactor to Mimic Hemodynamic Shear Stresses on Endothelial Cells in Microfluidic Systems Lightstone, Noam S. 26 June 2014 (has links) The mechanisms behind cardiovascular disease (CVD) initiation and progression are not fully elucidated. It is hypothesized that blood flow patterns regulate endothelial cell (EC) function to affect the progression of CVDs. A system that subjects ECs to physiologically-relevant shear stress waveforms within microfluidic devices has not yet been demonstrated, despite the advantages associated with the use of these devices. In this work, a bioreactor was designed to fulfill this need. Waveforms from regions commonly affected by CVDs including were derived. Pump motion and fluid flow profiles were validated by actuator motion tracking, particle image velocimetry, and flowmeters. While several relevant waveforms were successfully replicated, physiological waveforms could not be produced at physiological frequencies owing to actuator velocity and accuracy limitations, as well as dampening effects in the system. Overall, this work lays the foundation for designing a system that provides insight into the role of shear stress in CVD pathogenesis. Endothelial cell Bioreactor Flow loop Cardiovascular disease CVD Waveform Microfluidics Microfluidic devices Hemodynamics EC Shear stress Shear Pump Actuator Mechanical engineering Biomedical engineering PIV Particle image velocimetry Fluid dynamics Fluid mechanics Pressure Model Syringe Peristaltic Blood Blood flow Wall shear stress Womersley number Womersley Purday Physiological Flowmeter Sinusoid Sinusoidal Disturbed Non-disturbed Undisturbed Oscillatory Pulsatile In vivo In vitro 0548 0541
68	Pohybová skladba pro děti s poruchou autistického spektra / Motion Composition for children with Autistic Spectrum Disorder Menšíková, Marcela January 2014 (has links) Topic: Motion Composition for children with Autistic Spectrum Disorder Objectives: The aim of this thesis has been to find out the degree of mastery for individual performance of Motion Composition performed by children with Autistic Spectrum Disorder. Methods: Observation method was supplemented with video-recording that was conducted monthly. This was used to determine the degree of self-reliance. Also, feedback from other individuals has been used. Results: Outcomes of this survey indicate that children with Autistic Spectrum Disorder (ASD) are able to individually perform Motion Composition. Some children need more time to perform perfectly, but they are able to memorize components of Motion Composition, arrange them in the correct order and after some rehearsal they are able to perform Motion Composition correctly. The level of performance for individual components is raised proportionally when children can see visual execution of each element and also hear its description. Evaluated results of a second scientific question, which was related to the level of performance difficulty of individual components of Motion Composition for children with ASD, have brought unanticipated results. We anticipated that isometric exercises would be the most difficult components of Motion Composition. According...
69	The Development of the Parent Effectiveness Test (PET) Morrison, Sharon S. 01 December 1993 (has links) Abstract Not Available. Attention deficit hyperactivity disorder Education Educational evaluation Educational psychology Emotionally disturbed Learning disabilities School administration Special education Educational Psychology Special Education and Teaching
70	La mise en scène de l’intervention psychosociale en contexte de crise : ethnographie d’un partenariat « policier-travailleur social » Choinière-Tran, Kim 05 1900 (has links) No description available. Police État mental perturbé Santé mentale Travail social Approche dramaturgique Policing Emotionally disturbed person Social work Mental health Dramaturgic metaphor

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