No description available.

Polycystic Ovarian Syndrome (PCOS) is an endocrine disorder that affects women’s menstrual cycles, androgen (male hormones) levels, and cysts on the ovaries. This endocrine disorder has various symptoms, with insulin resistance as a hallmark symptom. Approximately 65-70% of women with PCOS have insulin resistance and hyperinsulinemia, whether or not they are overweight, obese, or lean (Marshall & Dunaif, 2012). Many women with PCOS struggle to lose weight because their excess weight is related to nutrition, lifestyle factors, and imbalanced hormones. Understanding PCOS as a metabolic disorder with nutritional implications led to investigating the potential benefit of having registered dietitian nutritionists (RDN) as part of the healthcare team of women with PCOS. We developed a survey based on the literature on PCOS, diet/nutrition interventions, and the role of RDNs in the healthcare team of PCOS women of childbearing age. Only childbearing-age women (18–44) diagnosed with PCOS were eligible to complete the survey. The survey was designed as a needs assessment to determine if women with PCOS are routinely referred to RDNs for support; whether or not such support is beneficial; and what gaps in knowledge or misconceptions about nutrition and PCOS exist among participants. Most importantly, it was designed to examine if women understand how nutrition relates to managing their PCOS symptoms and future disease risks. The data from this survey shows the need for RDNs on the healthcare team of women with PCOS and gives us an understanding of nutrition education interventions that could be developed for future studies. Understanding how RDNs play a role in symptom management could lead to a better quality of life for women with PCOS.

No description available.

No description available.

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, and late premolt) induced by multiple leg autotomy (MLA) in the blackback land crab, Gecarcinus lateralis. In all, 457 distinct protein spots were detected in the molting gland using two-dimensional gel electrophoresis, of which 230 (50%) changed significantly in abundance over the course of the molt cycle (one-way permutation ANOVA, p≤0.05). Changes in protein abundance were most notable between the intermolt and the three premolt stages, indicative of a biological ‘on-off’ switch in the Y-organ. Several hemolymph species proteins, including hemocyanin, cryptocyanin, and transglutaminase, were identified which characterized physiological changes associated with molting beyond the Y-organ. An abundance of cytoskeletal proteins were identified which correspond with glandular hypertrophy and are indicative of vesicular-mediated exocytosis, possibly of ecdysteroids. Further, several proteins involved in the immune, proteostasis, and oxidative stress response are characteristic of supporting the dynamic and demanding cellular changes associated with ecdysteroidogenesis and the transition of the Y-organ from the basal to the highly active state. Many proteins involved in energetic pathways including glycolysis, the citric acid cycle, amino acid metabolism, and one-carbon metabolism changed in abundance in response to both the higher energy demands and the requirement for precursors of macromolecular synthesis of the YO over the molt cycle. Taken together, these changes in diverse physiological pathways represent the complexity involved with regulation of the Y-organ, even with just the single proposed physiological purpose of ecdysteroidogenesis.

Humans experience ubiquitous exposures to estrogenic environmental chemicals from food, personal care products, and other industrial and consumer goods. Bisphenol A (BPA), a well-studied xenoestrogen, is known to alter development of estrogen-sensitive organs including the brain, reproductive tract, and mammary gland. Bisphenol S (BPS), which has a similar chemical structure to BPA, is also used in many consumer products, but its effects on estrogen-sensitive organs in mammals has not been thoroughly examined. In our study, pregnant CD-1 mice were orally exposed to BPS or ethinyl estradiol (EE2, a positive control for estrogenicity) from gestational day 9 through postnatal day (PND) 2, the period when many estrogen-sensitive organs are developing. After weaning, the offspring were administered either oil (vehicle) or an estrogen challenge (1 μg EE2/kg/day) for ten days starting at PND21 (prior to puberty), PND80 (early adulthood), or PND260 (later adulthood). Timing of puberty was evaluated in females by noting the date on which vaginal opening occurred. After the 10 day estrogen challenge, we evaluated the response of endocrine sensitive organs through measurements of organ weight, tissue morphology, and gene expression in both males and females. We observed dose- and sex-specific effects of BPS and EE2 treatment, as well as alterations in the responses of males and females to the estrogen challenge. This study sheds light on the effects of low dose xenoestrogen exposures on estrogen-sensitive organs including the reproductive tract and mammary gland. Furthermore, it improves our understanding of the influence of environmental chemicals on secular trends of earlier age of puberty in girls reported over the past few decades.

Bakgrund: Äldre patienter med typ 1-diabetes påträffas allt oftare i hemsjukvård då patienterna behöver hjälpinsatser. Vården för typ 1-diabetes har under flera år utvecklats vilket har resulterat i mindre komplikationer och längre överlevnad. Viktigt inom diabetesvården är patientens egenvård, men när andra sjukdomstillstånd tillkommer kan det vara svår att upprätthålla denna själv. Syfte: Syftet är att beskriva specialistsjuksköterskors erfarenheter av att möta äldre patienter med typ 1-diabetes i hemsjukvård där förmågan till egenvård har försämrats. Metod: En kvalitativ intervjustudie med induktiv ansats gjordes. Åtta specialistsjuksköterskor som arbetade inom hemsjukvård intervjuades med semistrukturerade frågor. Datamaterialet analyserades med en kvalitativ innehållsanalys. Resultat: Analysen resulterade i tre kategorier; Att se skillnaden mellan typ 1- och typ 2-diabetes hos patienten, Att kommunicera kunskap mellan sjukvårdspersonal och Att ta över ansvaret av patientens egenvård. Fynden i studien visade att det fanns skillnader att behandla patienter med typ 1- och typ 2-diabetes. Kunskap saknades om typ 1-diabetes och därmed var det svårt att förmedla information till omvårdnadspersonal. Att ta över ansvaret av patients egenvård beskrevs som en viktig, men svår del för att patienten ska känna sig trygg i relation med omvårdnadspersonal. Slutsats: Specialistsjuksköterskorna upplever en svårighet att ta över patientens egenvård när de inte längre har förmågan att själva utföra den. Utbildning inom diabetesvård är viktigt då specialistsjuksköterskan har som arbetsuppgift att utbilda, handleda och delegera omvårdnadspersonal. Typ 1-diabetes är en sjukdom som kommer att öka inom den äldre populationen, vilket innebär att patienterna kommer att ha ett behov av hemsjukvård i likhet med övrig befolkning. / Background: Elderly patients with type 1-diabetes are increasingly found in home healthcare as patients need assistance. Care for type 1-diabetes has developed over several years, which has resulted in fewer complications and longer survival. Important in diabetes care is the patient's self-care, but when other disease states arise, it can be difficult to maintain this yourself. Aim: The aim is to describe specialist nurses' experiences of meeting elderly patients with type1- diabetes in home care where the ability to self-care has deteriorated. Method: A qualitative interview study with inductive approach was performed. Eight specialist nurses who worked in home care were interviewed with semi-structured questions. The data material was analyzed using a qualitative content analysis. Results: The analysis resulted in three categories; To see the difference between type 1- and type 2-diabetes in the patient, To communicate knowledge between nursing staff and To take over the responsibility of self-care. The findings of the study showed that there were differences in treating patients with type 1- and type 2-diabetes. Knowledge of type 1 -diabetes was lacking and thus it was difficult to convey information to nursing staff. Taking over the patient's responsibility for self-care was described as an important, but hard part to make the patient feel safe in relation to nursing staff. Conclusion: The specialist nurses experience a difficulty in taking over the patient's self-care when the patients no longer are able. Diabetes care training is crucial as the specialist nurse's task is to train, supervise and delegate nursing staff. Type 1 diabetes is a disease that is estimated to increase in the elderly population, which means that patients will have a need for home care similar to the rest of the population.

For such a vexing topic as gender identity, this dissertation asks a rather straightforward question: If gender identity is—as Judith Butler has asserted—socially constructed and discursively mediated, then why does transgender hormone therapy (THT) work? This is the question concerning endocrinology that I ask Butler, and their answer is, if requiring of delicate assessment and interpretation, clear: it doesn't. Butler's work reveals an admonishing view that the efficacity of THT is due to placebo effect, in turn brought on by the bewitchment of the trans* who seeks medical transition. In a logic similar to sin and salvation, if only the trans* had not believed in gender dysphoria, then there would be no (putative) efficacity to THT whatsoever. With our answer, we begin a perilous adventure of discovering just why such a preeminent gender theorist (and trans* themselves) with no experience of gender dysphoria, and no desire to medically transition, would say this. We examine Butler's gender theory, their concept of desire, their views on the self, on transsexuality, their rarely discussed philosophies of science and nature, and their dearth of citations of transsexual voices. Due to this lack, I lend my own, relying upon my experience with gender dysphoria, THT, and medical transitioning. Unfortunately, in the times we live with attacks against *trans people and their healthcare, these questions could not be less theoretical, and more practical. In my view, Butler simply does not believe in the physical validity and medical exigency of gender dysphoria—the primary reason that we transsexuals pursue THT. Thus, in a dissertation that addresses questions of existential urgency and indisputable suffering, I show that Butler's gender theory lacks explanatory power and conceptual coherency, at a time when medically transitioning trans* could not need it more.

Brain-derived neurotrophic factor (BDNF), a neurotrophin involved in the regulation of food intake and body weight, has been implicated in the development and maintenance of Anorexia nervosa (AN). The majority of previous studies reported lower BDNF levels in acutely underweight AN patients (acAN) and increasing levels after weight rehabilitation. Here, we investigated serum BDNF concentrations in the largest known AN sample to date, both before and after weight restoration therapy. Serum BDNF was measured in 259 female volunteers: 77 in-patient acAN participants of the restrictive type (47 reassessed after short-term weight rehabilitation), 62 individuals long-term recovered from AN, and 120 healthy controls. We validated our findings in a post-hoc mega-analysis in which we reanalyzed combined data from the current sample and those from our previous study on BDNF in AN (combined sample: 389 participants). All analyses carefully accounted for known determinants of BDNF (age, sex, storage time of blood samples). We further assessed relationships with relevant clinical variables (body-mass-index, physical activity, symptoms). Contrary to our hypotheses, we found zero significant differences in either cross-sectional or longitudinal comparisons and no significant relationships with clinical variables. Together, our study suggests that BDNF may not be a reliable state- or trait-marker in AN after all.

Künstliches Licht in der Nacht (ALAN) entsteht in Zentren menschlicher Aktivität und erhellt die Nacht, wodurch biologische Rhythmen von Menschen und Wildtieren gestört werden können. Skyglow ist eine diffuse Aufhellung des Nachthimmels aufgrund von Reflexion und Streuung von ALAN, welche indirekt große Bereiche (vor-)städtischer Ökosysteme beleuchtet. Da sich Zentren menschlicher Aktivität häufig in der Nähe von Flüssen und Seen befinden, kann sich Skyglow unverhältnismäßig stark auf wildlebende Tiere in Süßwassergebieten auswirken. In drei Experimenten wurden die Auswirkungen von ALAN auf die Physiologie des Europäischen Flussbarsches untersucht. Die Fische wurden verschiedenen Versuchsbedingungen ausgesetzt: 1) niedrige ALAN-Intensitäten von 0,01, 0,1 und 1 lx unter kontrollierten Bedingungen, 2) höhere ALAN-Intensitäten von 1, 10 und 100 lx unter kontrollierten Bedingungen und 3) eine niedrige ALAN-Intensität von 0,06 lx in einem Feldexperiment. In den vorgestellten Experimenten unterdrückten niedrige ALAN-Intensitäten den nächtlichen Melatoninspiegel sowie teilweise Reproduktionshormone bei Weibchen. Höhere ALAN-Intensitäten verringerten das aktivste Schilddrüsenhormon und das relative Lebergewicht der Fische. Diese Arbeit zeigt physiologische Veränderungen bereits bei schwachen ALAN-Intensitäten, wie sie in großen Bereichen (vor-)städtischer Ökosysteme in Form von Skyglow vorkommen. Die empfindlichste Reaktionsvariable auf die Belastung durch ALAN bei Fischen ist der nächtliche Melatoninspiegel. Mögliche Wirkungen von ALAN auf andere physiologische Parameter können durch direkten Lichteinfall oder indirekt über reduziertes Melatonin ausgelöst werden. Diese Arbeit trägt zum Verständnis der Schwellenwerte für verschiedene physiologische Effekte durch eine mehrwöchige ALAN-Exposition bei. Schwellenwerte für ALAN-Intensitäten könnten zukünftig notwendige Deskriptoren für die Ausarbeitung von regulierenden Maßnahmen zur Reduzierung von Lichtverschmutzung liefern. / Artificial light at night (ALAN) is emitted from centers of human activities and increasingly brightens up nights, which can disturb biological rhythms of humans and wildlife. Skyglow is a diffuse brightening of the night sky due to reflection and scattering of ALAN, which indirectly illuminates large areas of (sub-)urban ecosystems. As centers of human activities are usually located close to rivers and lakes, skyglow may disproportionally affect wildlife of freshwater. Three experiments tested for effects of ALAN on the physiology of Eurasian perch. Fish were exposed 1) to low nocturnal illuminances of 0.01, 0.1 and 1 lx under controlled conditions, 2) to higher nocturnal illuminances of 1, 10 and 100 lx under controlled conditions, and 3) to low nocturnal illuminance of 0.06 lx in a field experiment. In the presented experiments, low nocturnal illuminance suppressed nocturnal melatonin production and reduced reproductive hormones to some extent in females. Higher nocturnal illuminance reduced the most active thyroid hormone and reduced relative liver weight of the fish. This thesis shows physiological changes already at very weak intensities of ALAN, like they occur over large areas of (sub-)urban ecosystems in the form of skyglow. The most sensitive response variable to ALAN exposure is the nocturnal melatonin levels. Possible actions of ALAN on other physiological parameters can be either by direct perception of light or indirectly via reduced melatonin. This thesis contributes to an understanding of thresholds for several physiological effects caused by ALAN exposure of several weeks. Thresholds for ALAN intensities could provide the necessary descriptors for elaborating regulatory measures to reduce light pollution in the future.