Submitted by Helmut Patrocinio (hell.kenn@gmail.com) on 2017-11-19T18:33:02Z No. of bitstreams: 1 Tarcisio_Velho_TESE_2008.pdf: 3588366 bytes, checksum: 527ea32ab36807e796e40f24a77425a5 (MD5) / Approved for entry into archive by Ismael Pereira (ismael@neuro.ufrn.br) on 2017-11-22T12:06:30Z (GMT) No. of bitstreams: 1 Tarcisio_Velho_TESE_2008.pdf: 3588366 bytes, checksum: 527ea32ab36807e796e40f24a77425a5 (MD5) / Made available in DSpace on 2017-11-22T12:07:03Z (GMT). No. of bitstreams: 1 Tarcisio_Velho_TESE_2008.pdf: 3588366 bytes, checksum: 527ea32ab36807e796e40f24a77425a5 (MD5) Previous issue date: 2008-06 / The consolidation of long-lasting sensory memories requires the activation of gene expression programs. However, to fully understand the contribution of genomic events necessary for sensory learning, it is necessary to characterize the components involved in this response, their induction kinetics, and the possible functional interactions among individual components. Birdsong represents one of the best natural behaviors to study gene expression induced by auditory stimulation in awake, freely behaving animals. The present work addresses the properties and functional consequences of the genomic response to birdsong in zebra finches (Taeniopygia guttata), with a specific focus on the caudomedial nidopallium (NCM), a cortical-like auditory area involved in song auditory processing and memorization. We found that song induces a wellorchestrated cascade of gene expression that includes early and late genes. Early genes are co-expressed by NCM neurons and their induction is regulated by the mitogen-activated protein (MAP) kinase pathway. In addition, we also found that the induction of early genes is locally modulated by norepinephrine. We have also identified two late genes, syn2 and syn3, whose induction is repressed by early song-induced proteins genes in NCM. Functionally, we show that blockade of this response by ?-adrenergic antagonists disrupts the long-term maintenance of long-lasting neuronal changes triggered by song. Finally, we show that the pattern of early song-induce gene expression in NCM is stimulus dependent. These patterns also indicate the existence of functional subdomains of more or less selectivity towards conspecific songs. Overall, these results help us understand the functional significance of the genomic response to song and the functional organization of sound representation in a higher order auditory area of the songbird brain. / The consolidation of long-lasting sensory memories requires the activation of gene expression programs. However, to fully understand the contribution of genomic events necessary for sensory learning, it is necessary to characterize the components involved in this response, their induction kinetics, and the possible functional interactions among individual components. Birdsong represents one of the best natural behaviors to study gene expression induced by auditory stimulation in awake, freely behaving animals. The present work addresses the properties and functional consequences of the genomic response to birdsong in zebra finches (Taeniopygia guttata), with a specific focus on the caudomedial nidopallium (NCM), a cortical-like auditory area involved in song auditory processing and memorization. We found that song induces a wellorchestrated cascade of gene expression that includes early and late genes. Early genes are co-expressed by NCM neurons and their induction is regulated by the mitogen-activated protein (MAP) kinase pathway. In addition, we also found that the induction of early genes is locally modulated by norepinephrine. We have also identified two late genes, syn2 and syn3, whose induction is repressed by early song-induced proteins genes in NCM. Functionally, we show that blockade of this response by ?-adrenergic antagonists disrupts the long-term maintenance of long-lasting neuronal changes triggered by song. Finally, we show that the pattern of early song-induce gene expression in NCM is stimulus dependent. These patterns also indicate the existence of functional subdomains of more or less selectivity towards conspecific songs. Overall, these results help us understand the functional significance of the genomic response to song and the functional organization of sound representation in a higher order auditory area of the songbird brain.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:02Z No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:22Z (GMT) No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:40Z (GMT) No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-01-06T13:30:40Z (GMT). No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Atualmente, devido as limita??es de expans?o das tradicionais ?reas de plantio de eucalipto, v?rios grupos de pesquisa t?m buscado estrat?gias para ampliar as fronteiras florestais brasileiras. Entretanto, a baixa disponibilidade h?drica dessas regi?es fronteiri?as tem limitado o desenvolvimento de alguns gen?tipos de eucalipto. ? vista disso, objetivou-se: i) avaliar as respostas morfofisiol?gicas de dois gen?tipos de eucalipto contrastantes quanto ? suas capacidades de tolerar o d?ficit h?drico e ii) construir uma biblioteca g?nica diferencial do gen?tipo tolerante ? seca, de maneira a possibilitar ? compreens?o dos mecanismos subjacentes ? toler?ncia ao d?ficit h?drico do ponto de vista gen?tico-molecular. O experimento foi conduzido, em casa de vegeta??o na UFVJM, em Diamantina, MG. As plantas dos dois gen?tipos, clones 953 (Eucalyptus camaldulensis vs. Eucalyptus grandis) e 224 (Eucalyptus grandis vs. Eucalyptus urophylla), modelo de toler?ncia e sensibilidade ao d?ficit h?drico, respectivamente, foram aclimatadas em casa de sombra, com irriga??o, durante 30 dias. Ap?s esse per?odo, foram submetidos a dois diferentes regimes h?dricos (irrigado e n?o irrigado), quando, ent?o, foram avaliados, ao longo do per?odo experimental, o crescimento em altura e di?metro, ?rea foliar, ?ndice de clorofila total, vari?veis da fluoresc?ncia da clorofila a, vari?veis de trocas gasosas, mat?ria seca de todos os compartimentos da planta e suas rela??es derivadas (rela??o mat?ria seca raiz e parte a?rea, raz?o de massa foliar, ?rea foliar espec?fica e raz?o de ?rea foliar), al?m da sintomatologia do estresse. O experimento foi conduzido em um Delineamento Inteiramente Casualizado, em esquema fatorial 2 (gen?tipos) x 2 (regimes h?dricos), totalizando quatro tratamentos (25 repeti??es cada). As an?lises estat?sticas foram realizadas mediante an?lise de vari?ncia. Para o estudo da express?o g?nica, concomitantemente a aplica??o do estresse h?drico, folhas foram coletadas, congeladas em N2 l?quido e armazenadas a - 80 ?C. Em seguida, procedeu-se com as extra??es e purifica??es do RNA total e, posterior, constru??o da biblioteca subtrativa. A defici?ncia h?drica n?o limitou o crescimento em altura dos gen?tipos estudados, embora, tenha reduzido o di?metro e a produ??o de mat?ria seca total. As demais vari?veis morfofisiol?gicas e todas as vari?veis de trocas gasosas foram negativamente afetadas pelo d?ficit h?drico. O clone 224 mostrou-se menos eficiente em termos fotossint?ticos e mais sens?vel ? restri??o h?drica para a maioria das vari?veis analisadas. Em contrapartida, o 953 apresentou maior toler?ncia ? falta d'?gua. Possivelmente, ?rea foliar reduzida, aumentos no teor de clorofila e aparelho fotossint?tico mais eficiente podem ter sido determinantes nessa maior toler?ncia encontrada no clone 953. Essas estrat?gias, podem, portanto, serem alvos em programas de sele??o de materiais gen?ticos mais tolerantes ? seca. Nesse sentido, a efici?ncia qu?ntica do Fotossistema II mostrou-se uma caracter?stica a ser considerada num processo de sele??o para toler?ncia ? falta d'?gua. A biblioteca de cDNA constru?da permitir? identificar quais genes est?o envolvidos nesse processo de toler?ncia no gen?tipo 953. Futuramente, esses genes candidatos poder?o ser transferidos para gen?tipos de interesse econ?mico, por meio de transgenia. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Currently, due to the limitations of expanding areas traditional of eucalyptus, several research groups have pursued strategies to expand the boundaries Brazilian forest. However, the low water availability of these border regions has limited the development of some genotypes of eucalyptus. Therefore, we aimed to: i) evaluate the morphological and physiological responses of two contrasting genotypes of eucalyptus as to their ability to tolerate drought and ii) build a library differential gene genotype drought tolerant, in order to enable the understanding molecular mechanisms underlying tolerance to water deficit. The experiment was conducted in a greenhouse at UFVJM in Diamantina, MG. The plants of the two genotypes, 953 clones (Eucalyptus camaldulensis vs. Eucalyptus grandis) and 224 (Eucalyptus grandis vs Eucalyptus urophylla) model of tolerance and sensitivity to water stress, respectively, were acclimated in the shade, irrigated for 30 days. After this period, underwent two different water regimes (irrigated and non-irrigated), when then were evaluated over the experimental period, the growth in height and diameter, leaf area, total chlorophyll index, chlorophyll a fluorescence variables, gas exchange variables, dry matter of all plant parts and their relations derived (shoot/root dry weight ratios, leaf weight ratio, specific leaf area and leaf area ratio) and the symptoms of stress. The experiment was conducted in a completely randomized design in a factorial 2 (genotypes) x 2 (water regimes), totaling four treatments (25 replicates each). Statistical analyzes were performed by analysis of variance. For the study of gene expression, concomitant application of water stress, leaves were collected, frozen in liquid N2 and stored at - 80 ?C. Then proceeded with the extraction and purification of total RNA and subsequent construction of the subtractive library. Water stress did not limit the growth in height of the genotypes studied, although it reduced the diameter and total dry matter production. The other morphophysiological variables and all gas exchange variables were negatively affected by drought. Clone 224 was less efficient photosynthetic and more sensitive to water restriction for most variables. In contrast, the 953 was more tolerant to lack of water. Possibly, reduced leaf area, increases in chlorophyll content and photosynthetic apparatus more efficient may have been determinant to increase tolerance found in clone 953. These strategies can be targeted in selection programs of genetic materials more drought tolerant. In this sense, the quantum efficiency of Photosystem II showed a characteristic to be considered in the selection process for tolerance to water shortage. A cDNA library constructed will allow to identify which genes are involved in the process of tolerance on the genotype 953. In the future, these candidate genes may be transferred to genotypes of economic interest through transgenesis.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T14:01:55Z No. of bitstreams: 5 23.pdf: 695049 bytes, checksum: f10d086518436baec21fc294fdf00c09 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T12:48:17Z (GMT) No. of bitstreams: 5 23.pdf: 695049 bytes, checksum: f10d086518436baec21fc294fdf00c09 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T12:48:17Z (GMT). No. of bitstreams: 5 23.pdf: 695049 bytes, checksum: f10d086518436baec21fc294fdf00c09 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Considerado como um ?hotspot? mundial de biodiversidade, o Cerrado apresenta alta abund?ncia de esp?cies end?micas e possui mais de 11.627 esp?cies de plantas nativas j? catalogadas. Dentre elas, o pequizeiro (Caryocar brasiliense), esp?cie que possui grande import?ncia ambiental e social neste bioma. A expans?o da fronteira agr?cola e a intensiva explora??o do Cerrado, por?m, t?m colocado em risco a preserva??o e a variabilidade gen?tica da esp?cie. Al?m disso, o extrativismo intensivo do pequizeiro pode gerar perdas de material gen?tico, j? que quase todos os frutos de qualidade, oriundos de gen?tipos superiores, s?o coletados. Dessa forma, o objetivo do presente trabalho foi analisar o banco de matrizes de pequi, utilizando marcadores moleculares microssat?lites, com fins de melhoramento e conserva??o da esp?cie. Para a extra??o do DNA gen?mico, foram utilizadas amostras foliares de 20 matrizes de Caryocar brasiliense, das quais 16 oriundas do Parque Estadual do Rio Preto (S?o Gon?alo do Rio Preto ? MG) e as demais oriundas da Fazenda Experimental da UFVJM ? Campus Moura (Curvelo ? MG). Para a amplifica??o do DNA, foram testados dez oligonucleot?deos espec?ficos para o pequi. Ap?s a amplifica??o, os fragmentos de DNA foram separados em gel desnaturante de poliacrilamida 10% e ureia 6 M em TBE 1x.A partir da leitura dos g?is gerou-se uma matriz bin?ria em que os indiv?duos foram genotipados quanto ? presen?a (1) e aus?ncia (0) de bandas. Com essa matriz, atrav?s do programa estat?stico R, calcularam-se as dist?ncias gen?ticas de Jaccard e obteve-se o dendrograma. As dist?ncias gen?ticas variaram de 0,15 a 0,70. A an?lise de agrupamento, representada pelo dendrograma, permitiu inferir que as matrizes foram divididas em quatro grupos distintos. Dessa forma, o programa de melhoramento do pequizeiro poder? utilizar esses dados para o estabelecimento e avalia??o de testes de prog?nies, com fins de produ??o ou conserva??o. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:34Z No. of bitstreams: 5 36.pdf: 448536 bytes, checksum: a5dbf2e4dce517f8347874ee986cea66 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T18:39:06Z (GMT) No. of bitstreams: 5 36.pdf: 448536 bytes, checksum: a5dbf2e4dce517f8347874ee986cea66 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T18:39:06Z (GMT). No. of bitstreams: 5 36.pdf: 448536 bytes, checksum: a5dbf2e4dce517f8347874ee986cea66 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2011 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / O objetivo do presente trabalho foi estimar os efeitos de popula??es, matrizes e idade da planta na express?o de vari?veis f?sicas, qu?micas e f?sico-qu?micas de frutos de pequizeiro. Colheram-se frutos oriundos de 15 matrizes em Curvelo e 15 em S?o Gon?alo do Rio Preto, Minas Gerais. A amostragem foi feita de maneira a se ter, em cada popula??o, matrizes com diferentes di?metros de tronco, sendo este ?ltimo uma estimativa da idade da planta. Para todas as vari?veis foram tomados dez frutos por matriz e as vari?veis qu?micas e f?sico-qu?micas foram avaliadas em cinco matrizes de cada popula??o. Avaliaram-se, em cada fruto, as vari?veis f?sicas: peso total; peso do mesocarpo externo; peso total dos put?mens; peso m?dio dos put?mens; n? de put?mens; peso total de polpa; e peso m?dio de polpa. As vari?veis qu?micas e f?sico-qu?micas, avaliadas na polpa, foram: umidade; cinzas; lip?deos; prote?nas; carboidratos totais; pH; s?lidos sol?veis totais; e acidez total titul?vel. O efeito de matrizes foi altamente significativo para todas as vari?veis avaliadas (P<1%), enquanto o de popula??es foi apenas para duas vari?veis (5%<P<10%). As estimativas das correla??es entre as vari?veis f?sicas avaliadas foram todas positivas, algumas significativas, enquanto que para as vari?veis qu?micas e f?sico-qu?micas observaram-se estimativas positivas e negativas, a maioria n?o significativa. As estimativas das correla??es entre o di?metro do tronco e as vari?veis f?sicas foram todas positivas, significativas apenas para duas vari?veis (P<5%). Para as vari?veis qu?micas e f?sico-qu?micas, observaram-se correla??es positivas e negativas, por?m n?o significativas. A sele??o de matrizes no campo para as caracter?sticas avaliadas que apresentaram correla??es n?o significativas ou significativas positivas deve promover ganhos gen?ticos nos descendentes se estes forem obtidos por propaga??o vegetativa, o mesmo n?o se podendo afirmar para descendentes obtidos por via sexuada. A idade da matriz n?o deve alterar os ganhos gen?ticos para estas vari?veis. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT The present work aimed to estimate population, mother tree and plant age effects on the expression of physical, chemical and physic-chemical traits on pequi fruits Seeds were collected from two populations and fifteen mother trees in each population, one located at Curvelo and another at S?o Gon?alo do Rio Preto, both from Minas Gerais State. The sampling was done in a way to sample trees among a range of trunk diameters, the last trait being an estimate of the tree age. Physical traits, evaluated on each of ten fruits sampled from all harvested trees, were: fruit total weight; external mesocarp weight; total weight of put?mens; mean weight of put?mens; number of put?mens; total weight of pulp; and mean weight of pulp. Chemical and physic-chemical traits, evaluated in five trees from each population, were: humidity; ashs; lipids; proteins; total carbohydrate; pH; total soluble solids; and total acidity. The mother trees effects were significant for all traits (P<1%), while the populations were only for two traits (5%<P<10%). Estimates of correlations among physical traits were all positive and some of them significant. For chemical and physic-chemical traits there were positive and negative correlations, most not significant. Estimates of correlations between trunk diameter and each physical variable were all positive, being significant for two traits (P<5%). Positive and negative but not significant correlations were observed for chemical and physic-chemical traits. Mother tree selection for traits that showed non significant or significant and positive correlations with trunk diameter may promote genetic gain in their descendents for vegetative propagation but the same assertion can?t be said when propagation is carried out by seeds. Mother tree age is not expected to cause any effect in genetic gains for these traits.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-22T11:29:16Z No. of bitstreams: 2 valdecy_aparecida_rocha_cruz.pdf: 267581 bytes, checksum: 1e59b25dbde1c757d85d3e2e0af0d778 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-22T12:50:57Z (GMT) No. of bitstreams: 2 valdecy_aparecida_rocha_cruz.pdf: 267581 bytes, checksum: 1e59b25dbde1c757d85d3e2e0af0d778 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2015-12-22T12:50:57Z (GMT). No. of bitstreams: 2 valdecy_aparecida_rocha_cruz.pdf: 267581 bytes, checksum: 1e59b25dbde1c757d85d3e2e0af0d778 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2010 / Estimativas precisas e acuradas de par?metros gen?ticos e fenot?picos s?o de grande import?ncia para se alcan?ar progresso gen?tico em uma popula??o e se selecionar, de forma mais eficiente, os indiv?duos das linhagens espec?ficas nos programas de melhoramento gen?tico. Com isso, objetivou-se com este trabalho estimar os par?metros da curva de postura, utilizando os modelos de polin?mios segmentados e Wood (1967); e estimar par?metros gen?ticos e fenot?picos dos par?metros do modelo de polin?mios segmentados, assim como a tend?ncia gen?tica dos mesmos. Foram utilizados dados da produ??o de ovo semanal das 25 at? 64 semanas de idade provenientes de 2.398 aves de uma linha f?mea de frangos de corte. As curvas de postura foram ajustadas utilizando os modelos de polin?mios segmentados e Wood (1967). O modelo de polin?mios segmentados ? constitu?do pelos par?metros ?xp?- idade das aves ao pico de postura, ?P?- n?vel de produ??o no pico, ?s? - taxa de decr?scimo semanal na produ??o de ovos ap?s o pico, e ?t? - tempo entre o in?cio e o pico de postura. O modelo de Wood ? constitu?do pelos par?metros ?a? - taxa de produ??o inicial, ?b? taxa de acr?scimo at? o pico de produ??o, e ?c? taxa de decr?scimo ap?s o pico de produ??o. As estimativas das m?dias dos par?metros das curvas foram realizadas utilizando o PROC MEANS do SAS. A estima??o dos componentes de covari?ncias, herdabilidades, correla??es e os valores gen?ticos para os par?metros da curva de postura de polin?mios segmentados foram obtidas utilizando o ?software? MTDFREML e as tend?ncias gen?ticas por meio do PROC REG do SAS. A linhagem avaliada apresentou um pico de postura ?s 30,43 semanas de idade, sendo o n?vel de postura de 0,746 ovo/aves/dia, com uma m?dia de 3,08 semanas do in?cio da postura at? o pico de postura e a taxa de persist?ncia foi de 0,014 ovo/ave/semana. As m?dias estimadas para os par?metros do modelo de Wood (1967) foram de 0,0003 para taxa de produ??o inicial 2,9398 para taxa de acr?scimo de produ??o at? o pico e de 0,0767 para taxa de decr?scimo de produ??o ap?s o pico postura. As curvas ajustadas para os modelos de polin?mios segmentados e Wood (1967) permitiram evidenciar um baixo n?vel de produ??o no pico de postura. O modelo de polin?mios segmentados superestimou a persist?ncia ap?s o pico de postura e o modelo de Wood (1967) subestimou o final da produ??o. As m?dias das herdabilidades foram de baixa a m?dia magnitudes, 0,25 (xp), 0,18 (P), 0,17 (s) e 0,10 (t). Estes resultados indicam que a sele??o para o par?metro ?xp? teria uma melhor resposta que para os demais par?metros. As correla??es entre o par?metro ?t? e os demais par?metros da curva foram positivas variando de 0,21 at? 1,00. Essas correla??es indicam que os animais com um maior tempo entre in?cio e pico de postura tendem a apresentar uma maior idade ao pico de postura, maior n?vel de produ??o e maior queda de produ??o p?s-pico. As correla??es foram negativas entre os par?metros ?s? e ?xp? e ?s? e ?P? apresentando-se menor entre ?s? e ?xp? (-0,17) e maior entre ?s? e ?P? (-0,91), indicando que um aumento no n?vel de produ??o e idade ao pico de produ??o acarretaria uma menor queda de produ??o de ovos p?s-pico. Podemos constatar que pode ser promovido ganho gen?tico para os par?metros da curva de produ??o de ovos, por?m a sele??o para um aumento do n?vel de produ??o poder? levar a um aumento na idade ao pico de postura. Assim como, aves mais precoces ao pico poder?o apresentar menor persist?ncia. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010. / ABSTRACT Precise and accurate estimates of genetic and phenotypic parameters are of great importance to achieve genetic progress in a population and select more efficiently individuals of specific lines in breeding programs. Thus, the aim with this study was to estimate the parameters of the curve approach, using models of segmented polynomials and Wood (1967) and to estimate genetic and phenotypic parameters of the model parameters of segmented polynomials, as well as the genetic tendency of them. It had been used data from the weekly egg production from 2,398 birds in a line of female broilers, aging from 25 to 64 weeks. The curves of posture were adjusted using the models of segmented polynomials and Wood (1967). The segmented polynomial model consists of parameters "xp" - the peak age of the birds in attitude, "P" - at peak production level, "s"- a weekly rate of decrease in egg production after peak, and ?t? - time between onset and peak position. The parameters of the Wood model consist of "a"- rate initial production," b "rate of increase until the production peak, and "c" rate of decline after peak production. Estimates of means of parameters were performed using SAS PROC MEANS. Estimation of covariance components, heritability?s, correlations and breeding values for the parameters of the curve posture segmented polynomial were obtained using the software MTDFREML and genetic trends by PROC REG of SAS. The strain studied showed a peak position at 30.43 weeks of age, the level of posture of 0.746 egg / poultry / day, with an average of 3.08 weeks of posture to the peak position and the rate of persistence was 0.014 eggs / bird / week. The estimated averages for the parameters of the model of Wood (1967) was 0.0003 for rate initial production rate of 2.9398 for increased production to peak and 0.0767 for rate of decline of production after the peak position . The fitted curves for the models of segmented polynomials and Wood (1967) have highlighted a low level of production at peak production. The segmented polynomial model overestimated the persistence after the peak position and the model of Wood (1967) underestimated the final production. The heritability?s means were low to medium magnitude, 0.25 (xp), 0.18 (P), 0.17 (s) and 0.10 (t). These results indicate that selection for the parameter "xp" would have a better response than for the other parameters. The correlations between the parameter "t" and the other parameters of the curve were positive ranging from 0.21 to 1.00. These correlations indicate that animals with a longer time between onset and peak position tend to have a higher age at peak position, the highest level of production and greater decrease in post-peak production. Were negative correlations between the parameters "s" and "xp" and "s" and "P" presenting lower between "s" and "xp" (-0.17) and higher in "s" and "P" (-0.91), indicating that an increase in production level and age at peak production would result in a smaller drop in egg production after peak. It has been noted that genetic gains can be obtained for the curve parameters for egg production, but a selection focused on an increased level of production could lead to an increase in age at peak production. Just as birds that present earlier peak may have lower persistence.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T12:38:46Z No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T17:44:56Z (GMT) No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2016-01-05T17:44:56Z (GMT). No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2014 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Objetivou-se realizar a caracteriza??o morfol?gica e molecular de gen?tipos de batata-doce do banco de germoplasma da Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM). Para a caracteriza??o morfol?gica, o experimento foi conduzido no Setor de Olericultura da UFVJM sendo avaliados 60 gen?tipos de batata-doce, utilizando 24 descritores morfol?gicos, tanto da parte a?rea quanto da parte radicular. As avalia??es da parte a?rea foram realizadas 60 e 90 dias ap?s o plantio, e as avalia??es da parte radicular foram realizadas ap?s a colheita aos 150 dias ap?s o plantio. Para a caracteriza??o molecular foram utilizadas amostras foliares de 60 acessos oriundos do banco de germoplasma-UFVJM. A extra??o do DNA foi realizada no Laborat?rio de Gen?tica e Biotecnologia Florestal (UFVJM), utilizando o m?todo Fenol-Clorof?rmio. Para a amplifica??o do DNA, foram utilizados onze primers microssat?lites espec?ficos para a batata-doce. A diversidade morfol?gica e molecular foi obtida por meio da gera??o de matrizes de dissimilaridade baseando-se no coeficiente de coincid?ncia simples e ?ndices de Jaccard, respectivamente. Para o estudo da diverg?ncia recorreu-se ao dendrograma ilustrativo obtido pelo m?todo UPGMA e ao agrupamento de Tocher. Para as matrizes de dissimilaridade obtida com os dados morfol?gicos e moleculares foram estimadas dist?ncias gen?ticas variando de 0,08 a 0,92 com m?dia 0,52 e 0,14 a 1, com m?dia 0,76, respectivamente. Observou-se pelos dendrogramas de dissimilaridade e pelo agrupamento de Tocher, que os acessos de batata-doce do banco de germoplasma da UFVJM apresentaram diversidade gen?tica e fenot?pica, sendo promissores em programas de melhoramento desta cultura. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT The objective was to make the morphological and molecular characterization of sweet potato genotypes of the germplasm bank of the Federal University of the Jequitinhonha and Mucuri Vales (UFVJM). For morphological characterization, the experiment was conducted in the Horticulture Sector of UFVJM being evaluated 60 sweet potato genotypes, using 24 morphological descriptors of both the shoots as the root part. The shoot evaluations were performed 60 and 90 days after planting, and ratings of roots were done after harvest at 150 days after planting. The molecular characterization leaf samples from 60 hits coming from the germplasm bank-UFVJM were used. DNA extraction was performed in Forest Genetics and Biotechnology Laboratory (UFVJM) using the phenol-chloroform method. For amplification of DNA eleven microsatellite primers specific for the sweet potato were used. The morphological diversity and Molecular was obtained by generating dissimilarity matrices based on the simple matching coefficient of Jaccard indices, respectively. To study the divergence appealed to the illustrative dendrogram by UPGMA and Tocher grouping. For dissimilarity matrices obtained with morphological and molecular data were estimated genetic distances ranging from 0,08 to 0,92 with a mean 0,52 and 0,14 to 1, with mean 0,76, respectively. It was observed by dendrograms of dissimilarity and the Tocher group, the sweet potato accessions of germplasm bank of UFVJM showed genetic and phenotypic diversity, and promising in breeding programs of this culture.

Made available in DSpace on 2014-12-17T14:03:29Z (GMT). No. of bitstreams: 1 SergioRFA.pdf: 2472351 bytes, checksum: daab3b710d9abca3798e5b5314990137 (MD5) Previous issue date: 2008-08-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Background: Leprosy can cause severe disability and disfigurement and is still a major health in different parts of the world. Only a subset of those individuals exposed to the pathogen will go on to develop clinical disease and there is a broad clinical spectrum amongst leprosy patients. The outcome of infection is in part due to host genes that influence control of the initial infection and the host?s immune response to that infection. Aim: Evaluate if polymorphisms type SNP in the 17q118q21 chromosomic region contribute to development of leprosy in Rio Grande do Norte population. Material and methods: A sample composed of 215 leprosy patients and 229 controls drawn from the same population were genotyped by using a Snapshot assay for eight genes (NOS2A, CCL18, CRLF3, CCL23, TNFAIP1, STAT5B, CCR7 and CSF3) located in chromosomic region 17q118q21. The genotype and allele frequency were measured and statistical analysis was performed by chi-square in SPSS version 15 and graph prism pad version 4 software. Results: Ours results indicated that the markers NOS2A8277, NOS2A8rs16949, CCR78rs11574663 and CSF38rs2227322 presented strong association with leprosy and their risk genotype were GG, TT, AA and GG respectively. The risk genotypes for all markers associated to leprosy presented recessive inheritance standard. When we compared the interaction among the markers in different combination we find that the marker NOS2A8277 associated with CCR78rs11574663 presented highest risk probability to development of leprosy. When we evaluated the haplotype of the risk markers it was found a haplotype associated with increase of the protection (CSF38rs22273228CC, CCR78 rs115746638GA, NOS2A8rs169498CT and NOS2A82778GA). The association of the clinical forms paucibacilary and multibacilary with markers showed that to the markers NOS2A8 2778GG, CCR78rs115746638AA and CSF38rs22273228GG there were a strong influence to migration to multibacilary pole and to marker NOS2A8rs169498TT the high proportion was found to the paucibacilary form. Conclusions: Changes in the genes NOS2A, CCR7 and CSF3 can influence the immune response against Mycobacterium leprae. The combination among these polymorphisms alters the risk probability to develop leprosy. The markers type SNP associated to development of the leprosy also are linked to clinical forms and its severity being the polymorphism NOS2A8rs169498TT associated with paucibacilar form and the polymorphisms NOS2A82778GG, CCR78rs115746638AA and CSF38rs22273228GG associated to multibacilar form / Introdu??o: A hansen?ase ? uma doen?a milenar que pode causar incapacidades f?sicas e desfiguramento, sendo ainda imponente em nosso pa?s, um problema de sa?de p?blica em seis pa?ses incluindo o Brasil. Apenas uma fra??o dos indiv?duos expostos ao Mycobacterium leprae desenvolve sintomas caracter?sticos da hansen?ase. Os fatores relacionados ? evolu??o da infec??o para doen?a depende em parte de caracter?sticas gen?ticas do hospedeiro que influenciam o controle da infec??o e ? progress?o da resposta imune. Objetivo: Avaliar se polimorfismos localizados na regi?o cromoss?mica 17q118q21 est?o associados ? hansen?ase numa popula??o oriunda do Rio Grande do Norte. Material e m?todos: Foram estudados 215 pacientes de hansen?ase e 229 controles sendo genotipados por Snapshot. Foram estudados varia??es em oito genes (NOS2A, CCL18, CRLF3, CCL23, TNFAIP1, STAT5B, CCR7 e CSF3) localizados na regi?o cromoss?mica 17q118q21. As freq??ncias genot?picas e al?licas foram determinadas por contagem direta e as diferen?as na distribui??o dessas entre os casos e os controles foram avaliadas por qui-quadrado usando o pacote estat?stico SPSS vers?o 15, e Graph Prism Pad vers?o 4.0. Resultados: Nossos resultados mostraram que os marcadores NOS2A8277, NOS2A8rs16949, CCR78rs11574663 e CSF38rs2227322 apresentam forte associa??o com a hansen?ase e seus gen?tipos de risco foram GG, TT, AA e GG respectivamente, apresentando todos padr?o de heran?a recessivo. O marcador NOS2A8277 e CCR78rs11574663 indicou maior probabilidade de risco no desenvolvimento a hansen?ase em (OR = 3,92, p = 0,0001). A avalia??o e hapl?tipos mostrou que CSF38rs22273228CC, CCR78rs115746638GA, NOS2A8rs169498CT e NOS2A82778GA est?o relacionados com a prote??o para o desenvolvimento da doen?a. Quando analisamos a distribui??o genot?pica dos marcadores estudados entre as formas cl?nicas paucibacilar e multibacilar, foram encontrados que os marcadores NOS2A82778 GG, CCR78rs115746638AA e CSF38rs22273228GG apresentavam uma forte associa??o com o polo multibacilar enquanto que o marcador NOS2A8rs169498TT foi associado a forma paucibacilar. Conclus?es: Os genes NOS2A, CCR7 e CSF3 possuiram grande import?ncia na resposta imune contra o Mycobacterium leprae e que modifica??es na seq??ncia nucleot?dica desses genes alteram a forma como agem no controle e desenvolvimento da hansen?ase. Os polimorfismos nesses genes possuem uma maior probabilidade de risco quando analisados combinados. A maior susceptibilidade induzida pelos polimorfismos nesses genes est? ligada a forma como evolui a doen?a sendo alguns deles associados a uma doen?a mais severa e outros a forma mais branda. Estes dados validam que o agregado de genes presentes no cromossomo 17 que expressam mol?culas importantes para manuten??o do equil?brio imune e que contribuem de forma intensa para a prote??o contra microorganismos intracelulares como o Mycobacterium leprae podem ter suas fun??es comprometidas por altera??o de suas seq??ncias nucleot?dicas

Made available in DSpace on 2014-12-17T14:03:34Z (GMT). No. of bitstreams: 1 LeonardoCF_DISSERT_1_30.pdf: 1416070 bytes, checksum: 28ab6b39aaaf4f25a511c52c766f780f (MD5) Previous issue date: 2010-08-02 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Preeclampsia is a multifactorial disease of unknown etiology that features with wide clinical symptoms, ranging from mild preeclampsia to severe forms, as eclampsia and HELLP syndrome. As a complex disease, preeclampsia is also influenced by genetic and environmental factors. Aiming to identify preeclampsia susceptibility genes, we genotyped a total of 22 genetic markers (single nucleotides polymorphisms SNPs) distributed in six candidates genes (ACVR2A, FLT1, ERAP1, ERAP2, LNPEP e CRHBP). By a case-control approach, the genotypic frequencies were compared between normotensive (control group) and preeclamptic women. The case s group was classified according to the disease clinical form in: preeclampsia, eclampsia and HELLP syndrome. As results we found the following genetic association: 1) ACVR2A and preeclampsia; 2) FLT1 and severe preeclampsia; 3) ERAP1 and eclampsia; 4) FLT1 and HELLP syndrome. When stratifying preeclampsia group according to symptoms severity (mild and severe preeclampsia) or according to the time of onset (early and late preeclampsia), it was detected that early preeclampsia is strongly associated to risk preeclampsia, eclampsia and HELLP syndrome have different genetic bases, although FLT1 gene seems to be involved in preeclampsia and HELLP syndrome pathophisiology / A pr?-ecl?mpsia ? uma doen?a multifatorial de etiologia ainda desconhecida que apresenta um amplo espectro quanto ? gravidade dos sintomas, podendo variar da forma mais branda (pr?-ecl?mpsia leve) ?s formas mais severas (ecl?mpsia e s?ndrome HELLP). Atualmente sabe-se que a pr?-ecl?mpsia ? influenciada tanto por fatores ambientais quanto por fatores gen?ticos. Com o prop?sito de identificar genes de suscetibilidade ? doen?a, genotipamos um total de 22 marcadores gen?ticos distribu?dos em seis genes candidatos (ACVR2A, FLT1, ERAP1, ERAP2, LNPEP e CRHBP). Utilizando uma abordagem do tipo casocontrole, comparamos as freq??ncias genot?picas entre mulheres normotensas (controles) e mulheres com pr?-ecl?mpsia (casos). O grupo dos casos foi dividido de acordo a forma cl?nica da doen?a em: pr?-ecl?mpsia, ecl?mpsia e s?ndrome HELLP. Como resultado p?de-se constatar as seguintes associa??es gen?ticas: 1) ACVR2A e pr?-ecl?mpsia; 2) FLT1 e pr?-ecl?mpsia grave; 3) ERAP1 e ecl?mpsia; 4) FLT1 e s?ndrome HELLP. Ao estratificar o grupo da pr?-ecl?mpsia de acordo com a gravidade dos sintomas (pr?-ecl?mpsia leve ou grave) ou de acordo com o tempo de in?cio dos sintomas (pr?-ecl?mpsia precoce ou tardia), comprovamos que o grupo pr?-ecl?mpsia precoce est? fortemente associado aos gen?tipos de risco. Nosso trabalho sugere que a pr?-ecl?mpsia, ecl?mpsia e s?ndrome HELLP possuem bases gen?ticas distintas, embora o gene FLT1 pare?a estar envolvido na fisiopatologia da pr?-ecl?mpsia e s?ndrome HELLP

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-11-17T15:04:48Z No. of bitstreams: 1 CristianeGouveaFajardo_TESE.pdf: 3260869 bytes, checksum: 3186d2c1c1792abff2cba08644e7739f (MD5) / Approved for entry into archive by Elisangela Moura (lilaalves@gmail.com) on 2015-11-17T15:20:02Z (GMT) No. of bitstreams: 1 CristianeGouveaFajardo_TESE.pdf: 3260869 bytes, checksum: 3186d2c1c1792abff2cba08644e7739f (MD5) / Made available in DSpace on 2015-11-17T15:20:02Z (GMT). No. of bitstreams: 1 CristianeGouveaFajardo_TESE.pdf: 3260869 bytes, checksum: 3186d2c1c1792abff2cba08644e7739f (MD5) Previous issue date: 2014-02-28 / Cattleya granulosa Lindley ? uma orqu?dea end?mica de da Floresta Atl?ntica do litoral do Nordeste brasileiro. A facilidade de coleta, e distribui??o em ?reas costeiras de interesse econ?mico tornam suas popula??es um alvo constante da coleta predat?ria, que tamb?m sofrem degrada??o ambiental. Devido ao impacto gerado em suas popula??es, a esp?cie est? amea?ada. Este estudo teve como objetivos avaliar os n?veis de agrega??o espacial em uma popula??o preservada, analisar as rela??es filogen?ticas de C. granulosa com outras quatro esp?cies de Laeliinae (Brassavola tuberculata, C. bicolor, C. labiata e C. schofieldiana) e ainda avaliar a diversidade gen?tica remanescente de 12 popula??es de C. granulosa, por meio de marcadores ISSR. Verificou-se especificidade da ep?fita C. granulosa com um ?nico for?fito, indiv?duos arb?reos de Eugenia spp. C. granulosa possui padr?o espacial agregado, com maior densidade de vizinhos no raio de at? 5 m. Nas rela??es filogen?ticas, C. bicolor exibiu o maior ?ndice de diversidade gen?tica (HE = 0,219), enquanto C. labiata exibiu o n?vel mais baixo (HE = 0,132). A porcentagem de varia??o gen?tica entre as esp?cies (AMOVA) foi de 23,26%. A an?lise de componentes principais (PCA) mostrou diverg?ncia gen?tica entre as esp?cies unifoliadas e bifolioladas. A PCA tamb?m indicou uma rela??o estreita entre C. granulosa e C. schofieldiana, que ? considerada por muitos pesquisadores uma variedade de C. granulosa. No estudo de gen?tica de popula??es, todos os locos foram polim?rficos. A alta diferencia??o gen?tica das popula??es (?ST = 0,391; P < 0,0001) determinou a estrutura??o em nove grupos, conforme modelo Log-likelihood da an?lise Bayesiana, com padr?o similar no dendrograma (UPGMA) e PCA. Houve correla??o positiva e significativa entre as dist?ncias geogr?ficas e gen?ticas entre as popula??es (r = 0,794; P = 0,017), indicando o isolamento pela dist?ncia. Padr?es de diversidade al?lica sugerem a ocorr?ncia de gargalos populacionais na maioria das popula??es de C. granulosa (n = 8). Assim, a manuten??o da diversidade gen?tica da esp?cie est? diretamente relacionada com a conserva??o das unidades ou grupos que est?o espacialmente distantes. / Cattleya granulosa Lind is a large and endemic orchid in Atlantic Forest fragments in Northeast Brazil. The facility of collecting, uniqueness of their flowers, which have varying colors between green and reddish brown, and distribution in coastal areas of economic interest make their populations a constant target of predation, which also suffer from environmental degradation. Due to the impact on their populations, the species is threatened. In this study, we evaluate the levels of spatial aggregation in a preserved population, analyze the phylogenetic relationships of C. granulosa Lindl. with four other Laeliinae species (Brassavola tuberculata, C. bicolor, C. labiata and C. schofieldiana) and also to evaluate the genetic diversity of 12 remaining populations of C. granulosa Lindl. through ISSR. There was specificity of epiphytic C. granula Lindl. with a single host tree, species of Eugenia sp. C. granulosa Lindl. own spatial pattern, with the highest density of neighbors within up to 5 m. Regarding the phylogenetic relationships and genetic patterns with other species of the genus, C. bicolor exhibited the greatest genetic diversity (HE = 0.219), while C. labiata exhibited the lowest level (HE = 0.132). The percentage of genetic variation among species (AMOVA) was 23.26%. The principal component analysis (PCA) of ISSR data showed that unifoliate and bifoliolate species are genetically divergent. PCA indicated a close relationship between C. granulosa Lindl. and C. schofieldiana, a species considered to be a variety of C. granulosa Lindl. by many researchers. Population genetic analysis using ISSR showed all polymorphic loci. The high genetic differentiation between populations (?ST = 0.391, P < 0.0001) determined the structure into nine groups according to log-likelihood of Bayesian analysis, with a similar pattern in the dendrogram (UPGMA) and PCA. A positive and significant correlation between geographic and genetic distances between populations was identified (r = 0.794, P = 0.017), indicating isolation by distance. Patterns of allelic diversity suggest the occurrence of population bottlenecks in most populations of C. granulosa Lindl. (n = 8). Genetic data indicate that enable the maintenance of genetic diversity of the species is complex and is directly related to the conservation of different units or groups that are spatially distant.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-04T22:02:35Z No. of bitstreams: 1 LeonardoCapistranoFerreira_TESE.pdf: 3396619 bytes, checksum: bffb880b6907328ac1ce5051faaf28fd (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-07T21:08:47Z (GMT) No. of bitstreams: 1 LeonardoCapistranoFerreira_TESE.pdf: 3396619 bytes, checksum: bffb880b6907328ac1ce5051faaf28fd (MD5) / Made available in DSpace on 2016-01-07T21:08:47Z (GMT). No. of bitstreams: 1 LeonardoCapistranoFerreira_TESE.pdf: 3396619 bytes, checksum: bffb880b6907328ac1ce5051faaf28fd (MD5) Previous issue date: 2014-10-06 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / O desenvolvimento de doen?as complexas, como a pr?-ecl?mpsia, ? determinado por fatores ambientais e gen?ticos, al?m da poss?vel intera??o entre esses fatores. As doen?as hipertensivas da gravidez (DHGs) apresentam um amplo espectro cl?nico, que pode variar desde pr?-ecl?mpsia leve (hipertens?o e proteinuria) at? formas mais graves, como a ecl?mpsia (convuls?es) e s?ndrome HELLP (hem?lise, eleva??o das enzimas hep?ticas e plaquetopenia). O espectro cl?nico parece estar ligado a diferentes mecanismos patol?gicos. Este trabalho tem como objetivo identificar fatores (gen?ticos e ambientais) envolvidos no desenvolvimento das DHGs. Usando uma abordagem caso-controle, selecionamos um total de 1498 mulheres para os estudos epidemiol?gico e gen?tico, abrangendo 755 gr?vidas normotensas (controle); 518 pr?-ecl?mpsia; 84 eclampsia e 141 HELLP. As mulheres foram genotipados para 18 marcadores distribu?dos em cinco genes candidatos (FLT1, ACVR2A, ERAP1, ERAP2 e LNPEP). Como resultado das an?lises dos fatores ambientais, encontramos idade materna, paridade e o ?ndice de massa corporal pr?-gestacional como importantes fatores de risco associados ?s DHGs. As an?lises gen?ticas mostraram que os genes est?o associados de maneira fen?tipo-espec?fica: ACVR2A com pr?-ecl?mpsia precoce (rs1424954, p=0,002); FLT1 com s?ndrome HELLP (rs9513095, p=0,003), e ERAP1 com ecl?mpsia (rs30187, p=0,03). Nossos resultados sugerem que diferentes mecanismos gen?ticos, juntamente com fatores ambientais espec?ficos, contribuem na determina??o do espectro cl?nico das DHGs. Al?m disso, o refinamento fenot?pico parece ser um passo essencial na busca por genes de doen?as complexas / The development of complex diseases such as preeclampsia are determined by both environmental and genetic factors, but there is also interaction among these factors. Preeclampsia is a pregnancy-specific disorder characterized by de-novo hypertension and proteinuria after 20th week of gestation. There is a broad spectrum of clinical presentations related to hypertensive disorders of pregnancy (HDP) that can range from mild preeclampsia to eclampsia (seizures) or HELLP syndrome (Hemolysis, Elevation of Liver enzymes, Low Platelets). Those clinical outcomes might be linked to different pathological mechanisms. Our work aims to identify factors (i.e. genes and environmental) associated with the HDP?s clinical spectrum. Using a case-control approach, we selected a total of 1498 pregnant women for epidemiological and genetic studies, encompassing 755 normotensive (control); 518 preeclampsia; 84 eclampsia; and 141 HELLP. Women were genotyped for 18 SNPs across 5 candidate genes (FLT1, ACVR2A, ERAP1, ERAP2 and LNPEP). For the environmental factors, we found maternal age, parity status and pre-gestational body mass index as important risk factors associated with disease. Genes were associated in a phenotype-specific manner: ACVR2A with early preeclampsia (rs1424954, p=0.002); FLT1 with HELLP syndrome (rs9513095, p=0.003); and ERAP1 with eclampsia (rs30187, p=0.03). Our results suggest that different genetic mechanisms along with specific environmental factors might determine the clinical spectrum of HDP. In addition, phenotype refinement seems to be an essential step in the search for complex disease genes